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Antioxidant assays by reducing potential and 2,2-diphenyl-1-picrylhydrazyl radical scavenging techniques as affected by pH and ion concentrations pH和离子浓度对还原电位和2,2-二苯基-1-苦味酰肼自由基清除技术的影响
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061206
B. Oso, C. O. Ogidi
The aim of the study is to investigate the effect of varying pH and different metal ion concentrations on the analyses of antioxidants by reducing potential (RP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging techniques. The investigation was conducted by examining the effects of various pH values (4, 5, 6, 7, 8 and 9) and potassium chloride concentrations (50, 100, 150, 200, 250 and 300 ug/mL) on the optical densities of reducing potential and DPPH radical scavenging potential of aqueous infusions of Cassia alata (L.) Roxb. The determinations were also conducted on the extraction media with the intention of identifying the probable source of variation in the investigation. The antioxidant potentials for both the aqueous infusion and media were most efficient at the least pH 4.0. Moreover, the antioxidant potentials decrease as the ion concentrations increase. The study revealed that the colorimetric methods for the determinations of DPPH radical scavenging and RP could be liable to errors arising from slight changes in acidity and concentrations of the metal ions thus affecting the performance characteristics in terms of repeatability and reproducibility of reports and meaningful comparisons of antioxidant capacity of dietary products among different authors.
研究了不同pH和不同金属离子浓度对还原电位(RP)和2,2-二苯基-1-吡啶酰肼(DPPH)自由基清除技术分析抗氧化剂的影响。研究了不同pH值(4、5、6、7、8和9)和氯化钾浓度(50、100、150、200、250和300 ug/mL)对决明子溶液中还原电位和光密度和DPPH自由基清除电位的影响。Roxb。还对提取介质进行了测定,目的是确定调查中可能的变异来源。水溶液输注和培养基的抗氧化电位在pH≤4.0时最有效。此外,随着离子浓度的增加,抗氧化电位降低。研究表明,测定DPPH自由基清除率和RP的比色法容易因酸度和金属离子浓度的微小变化而产生误差,从而影响了报告的重复性和再现性的性能特征,以及不同作者对膳食产品抗氧化能力的有意义比较。
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引用次数: 4
Fatigue in Post-Polio Syndrome 脊髓灰质炎后综合症的疲劳
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061203
M. Motta, A. Quadros, L. Ferreira, A. Oliveira
Post-Polio Syndrome (PPS) is characterized by the appearance of new muscular symptoms or worsening of previous residual symptoms, several years after the acute polio. The most common symptoms include a trial of fatigue, deterioration in muscle strength and pain. Fatigue is one of the most frequent complaints of PPS and the majority of patients identify fatigue as their most troubling symptom. The aetiology of the symptom arouses great interest, mainly due to its multifactorial character, and can be divided into two components: peripheral (muscular) fatigue and central (general) or simultaneously-occurring fatigue. Considering the importance of the symptom and the limitations that can cause in PPS patients, it is important to consider a series of variables, so that it can be better understood, thus providing a more effective and specific treatment.
脊髓灰质炎后综合征(PPS)的特点是在急性脊髓灰质炎发生数年后出现新的肌肉症状或先前残留症状的恶化。最常见的症状包括疲劳、肌肉力量下降和疼痛。疲劳是PPS最常见的主诉之一,大多数患者认为疲劳是他们最困扰的症状。该症状的病因引起了极大的兴趣,主要是由于其多因素特征,可分为两部分:外周(肌肉)疲劳和中枢(全身)或同时发生的疲劳。考虑到PPS患者症状的重要性和可能引起的局限性,考虑一系列变量是很重要的,这样可以更好地了解它,从而提供更有效和更有针对性的治疗。
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引用次数: 3
Birds species composition and abundance of a relativelly undisturbed vegetation at Mount Pati, Lokoja, Kogi State 科吉州Lokoja Pati山相对未受干扰植被的鸟类种类组成和丰度
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061225
D. Tanko, Rita Chinweuba
Avifauna of a relatively undisturbed vegetation at Mount Patti of Lokoja was surveyed from January to June, 2018. Line transect and point count techniques were used for data collection. This was done by weekly visits to the site between 6:00 h and 10:00 h of the day. Bird identification was done with the aid of a pair of binoculars, Field Guide of birds of western Africa and recorded bird calls. Monthly variation and season variation in bird composition and abundance were compared using Shannon-Wiener diversity index and equitability index. There were 39 species in 21 families encountered during the survey. The Family Nectarinidae had the highest number of 5 species, followed by Columbidae and Estrildidae with 4 species each. A total of 1,148 individuals were encountered during the six months survey with village weaver having the highest encountered rate of 107 while yellow-throated Long-claw had the least encountered rate of 4 individuals. Fluctuation was observed in the monthly encountered rate of the birds. Diversity and equitability indices had their highest values of 5.24 and 1.51 in January. More birds (696) were encountered in the wet season than the dry season (452). Four species were seen only in the wet season. Species effort curve showed that there are more species to be discover. The number of species encountered in this study revealed that the mountain is rich composition of bird species, even though the site seems to favour species that show preference to disturbed areas. The on-going anthropogenic activities probably paved way to these species to move in. Monthly and seasonal fluctuation of bird species diversity and abundance may be due to a combination of physical and biological factors. The physical and biological factors observed at the site which we speculate could be reasons for the observed fluctuations include slash and burnt system of farming, fuel-wood harvesting, intensive sporting activities creation of foot paths etc. High encountered rate indicate abundance and high activity level of the species. The village weaver was the most encountered species probably because they are known to be lousy, move in flocks and breed in colonies. In view of the rich composition and abundance of bird species on Mount Patti and the site being a suitable breeding site for the weavers. We recommend further study of the place as there are yet more species to be discovered as indicated by the species effort curve. There is need for conservation procedures to be initiated at the place and this study should serve as a baseline to initiate such programme by the state government.
2018年1月至6月,对Lokoja Patti山相对未受干扰植被的鸟类进行了调查。数据采集采用样线法和点计数法。这是通过每周在每天6点到10点之间访问现场来完成的。鸟类鉴定是在一副双筒望远镜、西非鸟类野外指南和鸟类叫声记录的帮助下完成的。利用Shannon-Wiener多样性指数和公平度指数比较了鸟类组成和丰度的月变化和季节变化。调查共发现21科39种。以油桃科最多,有5种,其次是耧斗科和雌蛾科,各有4种。在6个月的调查中,共捕获1148只,其中村织蝽的捕获率最高,为107只,黄喉长爪蝽的捕获率最低,为4只。月遇鸟率有波动。多样性指数和公平性指数在1月份最高,分别为5.24和1.51。雨季发现的鸟类(696只)多于旱季(452只)。有四种只在雨季出现。物种努力曲线显示有更多的物种有待发现。本研究中发现的物种数量表明,尽管该地点似乎偏爱那些对受干扰地区表现出偏好的物种,但该山的鸟类物种组成丰富。正在进行的人类活动可能为这些物种的迁移铺平了道路。鸟类物种多样性和丰度的月度和季节性波动可能是物理和生物因素共同作用的结果。在现场观察到的物理和生物因素,我们推测可能是观察到的波动的原因,包括砍伐和燃烧的农业系统,燃料木材的收获,密集的体育活动,步行道的创建等。高偶遇率表明该物种的丰度和活动水平较高。村织工是最常见的物种,可能是因为它们很讨厌,成群结队,成群繁殖。鉴于佩蒂山上鸟类种类丰富,构成丰富,是织布鸟的理想繁殖地。我们建议对这个地方进行进一步的研究,因为根据物种努力曲线,还有更多的物种有待发现。有必要在该地区启动保护程序,这项研究应作为邦政府启动此类计划的基线。
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引用次数: 0
Physicochemical study of Delphinium denudatum Wall (Ranunculales: Ranunculaceae) and their antioxidant activity 毛茛科飞燕草(Delphinium denudatum)的理化性质及其抗氧化活性
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061215
A. Gupta, Mahfoozurrahman Khan, D. Khan
The drug jadwar known as Delphinium denudatum Wall (Ranunculales: Ranunculaceae), the jadwar consist of dried tuberous roots of D. denudatum. An annual glabrous or slightly downy herbs found is Western Himalayas from Kumaon to Kashmir at altitudes of 3,000 m to 4,500 m specially on grassy slopes. It is distinguished by its relatively small blue and violet color and it is one of the important drugs used as indigenous medicine in India, especially in where isolated from the petroleum ether-soluble fraction of roots of D. denudatum. Jadwar widely used in traditional unani system of medicine for the treatment of a variety of human ailments including epilepsy. In order to increase the bioavailability, the nanophytosome of the D. denudatum root aqueous fraction (DNP) was prepared, characterized and evaluation. The phospholipids complex of the obtain aqueous fraction (AF) was prepared with phospholipon 90H. The size of nanophytosome was determined by dynamic light scattering. HPTLC fingerprinting of the AF was also performed.
该药又称白玉飞燕壁(毛茛科:毛茛科),白玉飞燕壁由白玉飞燕的干块根组成。一种一年生无毛或微绒毛草本植物,产于喜马拉雅山脉西部,从Kumaon到克什米尔,海拔3000米至4500米,特别生长在草坡上。它以其相对较小的蓝色和紫色颜色而闻名,是印度土着药物的重要药物之一,特别是在从D. denudatum根的石油醚可溶性部分中分离出来的地方。Jadwar在传统的unani医学系统中广泛用于治疗包括癫痫在内的各种人类疾病。为了提高白骨精的生物利用度,对白骨精根水组分(DNP)的纳米植物体进行了制备、表征和评价。用磷酸90H制备得到的水馏分(AF)的磷脂络合物。采用动态光散射法测定纳米植物体的大小。同时对AF进行HPTLC指纹图谱分析。
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引用次数: 5
Residual effects of neem Azadirachta indica A. Juss. (Sapindales: Meliaceae) seed-based fertilizer and NPK on the performance of Basella alba L. (Caryophyllales: Basellaceae) plant 印楝的残留效应。种肥和氮磷钾对白Basella L. (Caryophyllales: Basellaceae)植物生产性能的影响
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061213
S. Adejoro, D. Arije, A. Adegaye
Organomineral formulations are low input technology fertilizers, which combine the attributes of both organic and inorganic fertilizers. A completely randomized design (CRD) pot experiment was conducted in the screen house of the Department of Crop, Soil and Pest Management, of the Federal University of Technology, Akure, to evaluate the residual effects of neem Azadirachta indica A. Juss. (Sapindales: Meliaceae) seed based fertilizer and NPK 20:10:10 on the growth, yield and nutritional quality of Basella alba L. (Caryophyllales: Basellaceae). Results showed that the plots previously treated with neem seed-based fertilizer especially at 150-300 kg/ha enhanced the growth, yield and nutritional quality of B. alba, and increased these parameter significantly (P < 0.05) compared to the NPK treated and the untreated soil samples. It was therefore concluded that the Neem seed based fertilizer can serve as a viable alternative to NPK chemical fertilizers especially in rotational cropping systems owing to its prolonged soil action.
有机配方是低投入的技术肥料,它结合了有机和无机肥料的属性。采用完全随机设计(CRD)盆栽试验,在阿库尔联邦理工大学作物、土壤与有害生物管理系的筛网室内对印楝的残留效果进行了评价。种肥和氮磷钾20:10:10对白Basella L.(石竹科:Basellaceae)生长、产量和营养品质的影响。结果表明:与未施用氮磷钾和未施用氮磷钾的土壤样品相比,施印楝籽肥特别是施150 ~ 300 kg/ hm2的土壤样品对白桦的生长、产量和营养品质均有促进作用,且显著提高(P < 0.05);因此,印楝种子肥料可作为氮磷钾化肥的可行替代品,特别是在轮作制度下,由于其长期的土壤作用。
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引用次数: 1
Assessing AquaCrop model to simulate soil water contents under semi arid climate of Central Tunisia 评估AquaCrop模式模拟突尼斯中部半干旱气候下土壤含水量
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061219
Ghazouani Hiba, Basma Latrech, M. Amel, C. Amani, B. Douh, G. Issam, A. Boujelben
The objective of the present study was to preliminary calibrate and validate AquaCrop model based on crop conservative parameters from the literature for plant growth and water stress thresholds. In addition, physical soil characteristics, root growth, duration of plant stages and atmospheric demands were introduced according to field measurements. Based on this preliminary calibration, simulated water contents were compared to a measured data set of water contents retrieved from deficit and full irrigation treatments on a potato cropped field during an experimental year of 2015. Statistical indexes were computed and finally this performance in simulating water contents were validated under independent measurements carried out during an experiments campaign on the same field on 2014. Moreover, the paper presents the experimental protocol followed for soil characterization, considered as a milestone component for this soil water contents prediction. Results showed, that under the followed preliminary calibration, the model was able to simulate water contents (0v). In general, values of Root Mean Square Error were lower than 0.03 cm3.cm-3 representing the magnitude of error of the time domain reflectometry probe. Moreover values of Nash coffecients were close to 1 confirming the goodness of fit between measured and estimated water contents. Once assessed, the model could be used to study effects of different irrigation strategies on dynamic of water contents aiming to increase water use efficiency.
本研究的目的是初步校准和验证AquaCrop模型,该模型基于文献中作物保守参数,用于植物生长和水分胁迫阈值。此外,根据田间实测资料,介绍了土壤物理特性、根系生长、各生育期持续时间和大气需氧量。基于这一初步校准,模拟水分含量与2015年试验年马铃薯种植区亏缺和完全灌溉处理的水分含量测量数据集进行了比较。计算了统计指标,最后在2014年同一地块的独立试验中验证了该模拟含水量的性能。此外,本文还提出了土壤表征所遵循的实验方案,认为这是土壤含水量预测的里程碑式组成部分。结果表明,在后续的初步标定下,该模型能够模拟0v的含水率。总体而言,均方根误差值均小于0.03 cm3。Cm-3表示时域反射探头的误差幅度。此外,纳什系数的值接近于1,证实了测量和估计含水量之间的拟合良好。该模型可用于研究不同灌溉策略对水分动态的影响,以提高水分利用效率。
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引用次数: 0
The potential of naturally occurring bacteria for the bioremediation of toxic metals pollution 天然细菌对有毒金属污染的生物修复潜力
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061205
A. Vashishth, Nimisha Tehri, Pawan Kumar
An increase in industrialization and various kind of human activities added a huge amount of toxic heavy metals in the soil. As a result, toxic heavy metals in the environment may be adversely affects human being and aquatic ecosystem. Thus, it is very essential to understand mechanism of bioremediation through eco-friendly agent i.e. bacteria. Accumulation of high metal concentrations in soil above threshold limit causes lethal to bacterial communities in the environment. Few bacteria develop resistance mechanism to tolerate these toxic heavy metals and contain various methods to respond the metal stress. The present review emphasizes to understand the mechanism of bacterial resistance against toxic metals. Moreover, mechanism of bioaugmentation, biosorption, and bioaccumulation methods also described clearly.
工业化的增加和各种人类活动增加了土壤中大量的有毒重金属。因此,环境中的有毒重金属可能会对人类和水生生态系统产生不利影响。因此,了解利用细菌等生态友好剂进行生物修复的机理十分必要。土壤中重金属浓度的积累超过阈值,会对环境中的细菌群落造成致命影响。很少有细菌产生耐药机制来耐受这些有毒重金属,并有多种方法来应对金属胁迫。本文的重点是了解细菌对有毒金属的耐药机制。并对生物增强机理、生物吸附和生物富集方法进行了阐述。
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引用次数: 2
Long-term trend analysis of climatic variables and reference evapotranspiration over different urban areas in Tunisia 突尼斯不同城市地区气候变量和参考蒸散量的长期趋势分析
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061218
Basma Latrech, H. Ghazouani, Lasram Asma, B. Douh, Mansour Mohsen, A. Boujelben
In this study, the trend analysis of annual climatic variables including Tmax, Tmin, Tmean, RHmean, WS and SR as well as FAO-56 PM ET0 were investigated in three locations in Tunisia during 1984-2007. The Mann-Kendall Test, the Sen's Slope Estimator and linear regression tests were used for the analysis. The obtained results showed a significant increasing trends (a < 0.001) and (a < 0.01) in annual Tmax, Tmin and Tmean at all the considered locations. However, Tmin increase faster than Tmax with a slope of magnitudes ranging between 0.057 to 0.1 oC year-1. For RHmean, a non-significant tendency of decrease was observed in Chott-Mariem station. However, significantly increasing trends were found for Kelibia and Tunis Carthage. Concerning the WS variable, a tendency of decrease is observed during the study period for all the stations. Nevertheless, the statistical analysis of decreasing tendency of wind speed varied from non-significant for Tunis Carthage to highly significant (a <= 0.001) at Chott-Mariem and Kelibia. Despite the highly significant upward trend of temperature, the temporal pattern of mean annual FAO56 PM-ET0, over the different stations, did not exhibit any significant trend except for Kelibia station.
本文对1984-2007年突尼斯3个地点的Tmax、Tmin、Tmean、RHmean、WS和SR以及FAO-56 PM ET0等气候变量进行了趋势分析。采用Mann-Kendall检验、Sen’s Slope Estimator和线性回归检验进行分析。结果表明,各试验点的年Tmax、Tmin和Tmean均有显著增加趋势(a < 0.001)和显著增加趋势(a < 0.01)。然而,Tmin的增加速度快于Tmax,斜率在0.057 ~ 0.1 oC -1之间。RHmean的Chott-Mariem站的下降趋势不显著。然而,在克里比亚和突尼斯迦太基,发现了显著增加的趋势。在研究期间,所有台站的WS变量都有减小的趋势。然而,风速下降趋势的统计分析从突尼斯迦太基的不显著到Chott-Mariem和Kelibia的高度显著(a <= 0.001)。除Kelibia站外,各站点年平均FAO56 PM-ET0的时间变化趋势均不显著。
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引用次数: 0
Using AquaCrop Model to simulate irrigation water use efficiency of potato crop under semi-arid conditions of Central Tunisia 利用AquaCrop模型模拟突尼斯中部半干旱条件下马铃薯作物灌溉用水效率
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061220
Ghazouani Hiba, Basma Latrech, B. Douh, C. Amani, M. Amel, G. Issam, A. Boujelben
In Tunisia, water scarcity forces producers to face stress conditions. In this study, AquaCrop was used to reproduce the dynamic of water contents, vegetative growth, yield production and water use efficiency under a non-stressed and water stressed treatments. Calibration procedure aimed to use in maximum default parameters of AquaCrop. Since, the paper presented only the parameters that have to be adjusted to obtain similar results of field measurements. Root mean squared error, RMSE, values were always lower than 0.04 cm3.cm-3 for water contents lower than 0.06 for vegetation cover estimation. Moreover, results from Nasch Coefficient, E, were almost equal to one. RMSE and E justified that the model was well assessed to predict the soil water contents and vegetation development under the study area. However, the model presented a greater performance in the case of full irrigation strategy. When evaluating different values of water productivity, it was showed that a WP of 32 g.m-2 produced the lowest estimation error. Regarding yield productions, statistical indictors, computed for a water productivity value of 32 g.m-2 show in general RMSE values lower than 0.4 t/ha. In addition, E was closer to one for the non stressed treatment, T1. For irrigation water use efficiency, it was depicted that the model underestimated field IWUE. Moreover, the discrepancy between simulated and estimated irrigation water use efficiency rose for treatment T2, implying that the model calibration should be improved, especially for stressed conditions. The model, after being calibrated, could be used for simulating the response of the crop to different irrigation management aiming to optimize water use efficiency.
在突尼斯,水资源短缺迫使生产者面临压力。本研究利用AquaCrop模拟了不同胁迫条件下水稻水分含量、营养生长、产量和水分利用效率的动态变化。校准程序旨在使用AquaCrop的最大默认参数。因此,本文只给出了需要调整的参数,以获得类似的现场测量结果。均方根误差(RMSE)值均小于0.04 cm3。Cm-3对植被覆盖度估算含水量小于0.06。此外,纳什系数E的结果几乎等于1。RMSE和E表明,该模型能够较好地预测研究区土壤含水量和植被发育。然而,该模型在完全灌溉策略下表现出更好的性能。在不同水分生产力值的估算中,以32 g m-2的WP估算误差最小。在产量方面,根据水分生产力值为32 g - m-2计算的统计指标显示,RMSE值一般低于0.4 t/ha。此外,对于非应力处理T1, E更接近于1。在灌溉用水效率方面,模型低估了农田IWUE。此外,T2处理灌溉水利用效率的模拟值与估计值之间的差异有所增大,表明模型的校准还有待改进,特别是在应力条件下。该模型经标定后,可用于模拟作物对不同灌溉管理方式的响应,以优化水分利用效率。
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引用次数: 0
Synthesis and in silico investigation of Schiff base derivatives of 1H-indole-2,3-diones and their Co(II) and Ni(II) complexes as antimicrobial agents h -吲哚-2,3-二酮席夫碱衍生物及其Co(II)和Ni(II)配合物的合成和硅研究
Pub Date : 2019-04-30 DOI: 10.21472/BJBS.061207
Helen O. Echekwube, P. Ukoha, O. T. Ujam, C. Nwuche, J. Asegbeloyin, A. Ibezim
3-[(2-aminophenyl)imino]-1,3-dihydro-2H-indol-2-one, (Lo), 1,3-phenylenediazanylylidene di (1,3-dihydro-2H-indol-2-one), (Lm) and 1,4-phenylenediazanylylidene di(1,3-dihydro-2H-indol-2-one) (Lp) were synthesized by the reaction of 1H-indole-2,3-dione with benzene-1,2-diamine, benzene-1,3-diamine and benzene-1,4-diamine respectively. The reaction of Lo, Lm and Lp with Co(II) and Ni(II) halides gave the corresponding coordination complexes which were characterized by elemental analysis, molar conductance, infra-red, GC-MS and electronic spectral studies. Docking of the 1H-indole-2,3-diones toward the binding sites of penicillin binding protein and DNA gyrase showed they interacted favourably with the test antibacterial targets at deltaGs range of -2.51 to -5.48 kcal/mol. In accordance to literature report, coordination of cobalt and nickel to the ligands yielded metal complexes which exhibited improved interaction with the protein targets (at deltaGs range of -8.70 to -10.20 kcal/mol). In vitro antimicrobial studies against some microorganisms showed that some of the compounds were active against few Gram negative and Gram positive bacteria. The Lo, Lm and Lp had no activity against any of the test microorganisms but the Co(II) and Ni(II) complexes, showed antibacterial activity. The [Co(Lo)2] and [Ni(Lo)2] complexes generated the least antibacterial response. [Co(Lo)2] was ineffective against E. coli 6 and Staphylococcus sciuri subsp sciuri while Bacillus subtilis was resistant to [Ni(Lo)2] which moderately inhibited E. coli 14 (7 mm). Both compounds indicated zero activity against Pseudomonas aeruginosa. The complex that evoked the highest bactericidal activity were [CoLm]Cl2 and [NiLp]Cl2. The antibiogram activity of [CoLm]Cl2 was found between 20 and 30 mm with E. coli 6 displaying greater sensitivity (30 mm) and S. sciuri the least (20 mm). The activity of [NiLp]Cl2 complex indicate that the activity spectrum of the organisms occurred within 29 and 45 mm range; the least sensitive were E. coli 14 (29 mm) and B. subtilis (29 mm) while the most sensitive was S. sciuri subsp sciuri (45 mm). The two compounds were further studied for minimum inhibitory concentration (MIC) and their binding modes towards the studied protein targets were analyzed. Result indicate that the MIC of 1.25 ug/mL was determined for the complex ([NiLp]Cl2) against S. sciuri subsp sciuri (12 mm) while in case of [CoLm]Cl2, the MIC was 2.5 ug/mL (13 mm) against the same organism. The binding modes predicted for [CoLm]Cl2 and [NiLp]Cl2 identified essential residues necessary for interaction with the studied proteins and which could be targeted during structural/activity optimization.
通过1h -吲哚-2,3-二酮与苯-1,2-二胺、苯-1,3-二胺和苯-1,4-二胺的反应,分别合成了3-[(2-氨基苯基)亚胺]-1,3-二氢- 2h -吲哚-2- 1、(Lo)、1,3-苯-2,3-二氢- 2h -吲哚-2- 1、(Lm)和1,4-苯-1,4-二胺。Lo、Lm和Lp与Co(II)和Ni(II)卤化物反应生成相应的配位配合物,通过元素分析、摩尔电导、红外、GC-MS和电子能谱研究对其进行了表征。1h -吲哚-2,3-二酮与青霉素结合蛋白和DNA旋切酶结合位点的对接表明,它们与抗菌靶点在-2.51 ~ -5.48 kcal/mol的deltaGs范围内具有良好的相互作用。根据文献报道,钴和镍与配体配位产生的金属配合物与蛋白质靶标的相互作用更好(在-8.70至-10.20 kcal/mol的deltaGs范围内)。体外抑菌实验表明,部分化合物对革兰氏阴性菌和革兰氏阳性菌均有抑菌活性。Lo、Lm和Lp对试验微生物均无抑菌活性,但Co(II)和Ni(II)配合物有抑菌活性。[Co(Lo)2]和[Ni(Lo)2]配合物产生的抗菌反应最小。[Co(Lo)2]对大肠杆菌6和sciuri次葡萄球菌无效,而枯草芽孢杆菌对[Ni(Lo)2]有抗性,对大肠杆菌14 (7 mm)有中等抑制作用。两种化合物对铜绿假单胞菌均无活性。抗菌活性最高的络合物是[CoLm]Cl2和[NiLp]Cl2。[CoLm]Cl2的抗生素谱活性在20 ~ 30 mm之间,大肠杆菌6表现出较高的敏感性(30 mm),而sciuri则表现出最低的敏感性(20 mm)。[NiLp]Cl2配合物的活性表明,生物的活性谱发生在29和45 mm范围内;最不敏感的是大肠杆菌14 (29 mm)和枯草芽孢杆菌(29 mm),最敏感的是sciuri subsp sciuri (45 mm)。进一步研究了这两种化合物的最低抑制浓度(MIC),并分析了它们与所研究蛋白靶点的结合模式。结果表明,复合物([NiLp]Cl2)对S. sciuri subsp sciuri (12 mm)的MIC为1.25 ug/mL,而复合物([CoLm]Cl2)对同一生物的MIC为2.5 ug/mL (13 mm)。预测的[CoLm]Cl2和[NiLp]Cl2的结合模式确定了与所研究的蛋白质相互作用所必需的基本残基,这些残基可以在结构/活性优化过程中作为目标。
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引用次数: 3
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Brazilian Journal of Biological Sciences
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