Agus Kresnadi, M. Edward, Gilson Khang, Heri Suroto
ABSTRACT Tendons that are crucial for movement are struggling with self-healing. Regenerative medicine has been using mesenchymal stem cells (MSCs) and their secretomes, and it offers promise. These secretomes, rich in growth factors, aid tissue healing. Tenogenic secretomes target tendon-specific healing, while crude ones provide a broader view of MSC signaling. The study aims to enhance targeted interventions in tendon regeneration and improve the quality of life for those with tendon-related issues. This laboratory study compares freeze-dried tenogenic and crude secretomes of MSCs under hypoxic conditions for regenerative potential. Stem cells from adipose tissue undergo isolation, culture, and multiplication then treated in hypoxic conditions. Characterization, Interleukin-6 (IL-6) and Interleukin-10 (IL-10) analysis with enzyme-linked immunosorbent assay (ELISA) follow. The results, including organoleptic, pH, and scanning electron microscope assessments, were presented descriptively. Statistical analysis will enhance understanding. Physical characteristics (odor, color, and pH) are similar in both groups. Tenogenic secretome has a more concave surface, potentially aiding cell migration compared to the crude secretome. There were significant differences in cytokine levels (P = 0.001). IL-6 levels expressed from tenogenic secretome were 10.2 ± 2.432 pg/mL compared to the crude one which 18.3 ± 3.175 pg/mL. Levels of IL-10 expressed from tenogenic and crude secretomes were 309.2 ± 50.973 pg/mL and 200.1 ± 32.030, respectively. The tenogenic secretome and crude secretome exhibit no differences in physical characteristics but differ in surface morphology. The concave surface of the tenogenic secretome facilitates cell migration more than the crude secretome. Under hypoxic conditions, the tenogenic secretome shows lower pro-inflammatory (IL-6) and higher anti-inflammatory (IL-10) effects than the crude secretome.
{"title":"Characteristic Differences between Freeze-dried Tenogenic Secretomes and Freeze-dried Crude Secretomes of Mesenchymal Stem Cells under Hypoxic Conditions","authors":"Agus Kresnadi, M. Edward, Gilson Khang, Heri Suroto","doi":"10.4103/bhsj.bhsj_52_23","DOIUrl":"https://doi.org/10.4103/bhsj.bhsj_52_23","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Tendons that are crucial for movement are struggling with self-healing. Regenerative medicine has been using mesenchymal stem cells (MSCs) and their secretomes, and it offers promise. These secretomes, rich in growth factors, aid tissue healing. Tenogenic secretomes target tendon-specific healing, while crude ones provide a broader view of MSC signaling. The study aims to enhance targeted interventions in tendon regeneration and improve the quality of life for those with tendon-related issues.\u0000 \u0000 \u0000 \u0000 This laboratory study compares freeze-dried tenogenic and crude secretomes of MSCs under hypoxic conditions for regenerative potential. Stem cells from adipose tissue undergo isolation, culture, and multiplication then treated in hypoxic conditions. Characterization, Interleukin-6 (IL-6) and Interleukin-10 (IL-10) analysis with enzyme-linked immunosorbent assay (ELISA) follow. The results, including organoleptic, pH, and scanning electron microscope assessments, were presented descriptively. Statistical analysis will enhance understanding.\u0000 \u0000 \u0000 \u0000 Physical characteristics (odor, color, and pH) are similar in both groups. Tenogenic secretome has a more concave surface, potentially aiding cell migration compared to the crude secretome. There were significant differences in cytokine levels (P = 0.001). IL-6 levels expressed from tenogenic secretome were 10.2 ± 2.432 pg/mL compared to the crude one which 18.3 ± 3.175 pg/mL. Levels of IL-10 expressed from tenogenic and crude secretomes were 309.2 ± 50.973 pg/mL and 200.1 ± 32.030, respectively.\u0000 \u0000 \u0000 \u0000 The tenogenic secretome and crude secretome exhibit no differences in physical characteristics but differ in surface morphology. The concave surface of the tenogenic secretome facilitates cell migration more than the crude secretome. Under hypoxic conditions, the tenogenic secretome shows lower pro-inflammatory (IL-6) and higher anti-inflammatory (IL-10) effects than the crude secretome.\u0000","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141103318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Primasari, I. D. Saputro, L. Hariani, Glorian Paul Bosco Velusamy
ABSTRACT An alternative for supporting wound closure is acellular dermal matrix (ADM), which serves as a scaffold. Humans and porcine possess a similar biochemical makeup. Using sodium dodecyl sulfate (SDS), a decellularization technique was developed and its biomechanical properties were assessed. This work uses a pig dermis layer for an in vitro experimental investigation with a posttest-only control group. Using SDS 0.5% for 14 days, the decellularization procedure compares the biomechanical properties and cellular components of the ADM with control. The Mann–Whitney U-test for data with a nonnormal distribution or the t-test for continuous variables with a normal distribution was used for the study. Histological analysis revealed that none of the cells were detected in four fields of analysis in the treatment group; however, 48.00 ± 4.86 cells were observed in the control group (P < 0.001); the collagen organization in the control group appeared to be identical. The variables elastic modulus (MPa) (136.78 vs. 129.19; P = 0.556), thickness (mm) (3.27 vs. 3.15; P = 0.397), and width (mm) (8.50 vs. 8.56; P = 0.40) did not differ statistically. The following data showed significant differences between the treatment group and the control group: break strain (%) (108.46 vs. 67.48; P < 0.001) and tensile strength stress (MPa) (19.916 vs. 22.1; P = 0.030). SDS decellularization is an efficient method for creating an ADM. Although the break strain was considerably lower, the treatment group’s tensile strength was higher. Elastic modulus changes were not observed.
摘要 支持伤口闭合的另一种方法是用作支架的非细胞真皮基质（ADM）。人和猪具有相似的生化结构。利用十二烷基硫酸钠（SDS）开发了一种脱细胞技术，并对其生物力学特性进行了评估。 这项工作使用猪的真皮层进行体外实验研究，并设置了一个只进行试验后对照组。脱细胞程序使用 0.5% 的 SDS，持续 14 天，将 ADM 的生物力学特性和细胞成分与对照组进行比较。对于非正态分布的数据采用曼-惠特尼 U 检验，对于正态分布的连续变量采用 t 检验。 组织学分析表明，治疗组在四个分析视野中均未检测到细胞；但对照组观察到 48.00 ± 4.86 个细胞（P < 0.001）；对照组的胶原组织似乎相同。弹性模量 (MPa) (136.78 vs. 129.19; P = 0.556)、厚度 (mm) (3.27 vs. 3.15; P = 0.397) 和宽度 (mm) (8.50 vs. 8.56; P = 0.40) 这些变量在统计学上没有差异。以下数据显示处理组与对照组之间存在显著差异：断裂应变（%）（108.46 对 67.48；P < 0.001）和拉伸强度应力（兆帕）（19.916 对 22.1；P = 0.030）。 SDS 脱细胞是创建 ADM 的有效方法。虽然断裂应变大大降低，但处理组的拉伸强度更高。未观察到弹性模量的变化。
{"title":"Developing Porcine Acellular Dermal Matrix by Using Sodium Dodecyl Sulfate and Biomechanical Property Testing","authors":"M. Primasari, I. D. Saputro, L. Hariani, Glorian Paul Bosco Velusamy","doi":"10.4103/bhsj.bhsj_2_24","DOIUrl":"https://doi.org/10.4103/bhsj.bhsj_2_24","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 An alternative for supporting wound closure is acellular dermal matrix (ADM), which serves as a scaffold. Humans and porcine possess a similar biochemical makeup. Using sodium dodecyl sulfate (SDS), a decellularization technique was developed and its biomechanical properties were assessed.\u0000 \u0000 \u0000 \u0000 This work uses a pig dermis layer for an in vitro experimental investigation with a posttest-only control group. Using SDS 0.5% for 14 days, the decellularization procedure compares the biomechanical properties and cellular components of the ADM with control. The Mann–Whitney U-test for data with a nonnormal distribution or the t-test for continuous variables with a normal distribution was used for the study.\u0000 \u0000 \u0000 \u0000 Histological analysis revealed that none of the cells were detected in four fields of analysis in the treatment group; however, 48.00 ± 4.86 cells were observed in the control group (P < 0.001); the collagen organization in the control group appeared to be identical. The variables elastic modulus (MPa) (136.78 vs. 129.19; P = 0.556), thickness (mm) (3.27 vs. 3.15; P = 0.397), and width (mm) (8.50 vs. 8.56; P = 0.40) did not differ statistically. The following data showed significant differences between the treatment group and the control group: break strain (%) (108.46 vs. 67.48; P < 0.001) and tensile strength stress (MPa) (19.916 vs. 22.1; P = 0.030).\u0000 \u0000 \u0000 \u0000 SDS decellularization is an efficient method for creating an ADM. Although the break strain was considerably lower, the treatment group’s tensile strength was higher. Elastic modulus changes were not observed.\u0000","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141102730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Serological testing for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is crucial for detecting antibodies generated in response to infection. Neutralizing antibodies (NAbs) target the receptor-binding domain (RBD) of the spike protein (S-RBD) and can exist in the forms of immunoglobulin G (IgG), IgM, and IgA. This study aimed to assess specific antibody responses to the nucleocapsid protein (N), S-RBD, and NAbs and correlate them with the clinical spectrum of coronavirus disease 2019 (COVID-19). A cross-sectional design was conducted at the Dr. Soetomo General Academic Hospital, Indonesia. We examined 48 patients with confirmed COVID-19 of varying severity (mild, moderate, severe, and critical) on treatment days 0, 3, and 6. We measured nucleocapsid antibodies, S-RBD, and neutralization using the chemiluminescence immunoassay method. The comparative analysis of IgM, IgG, NAB, and S-RBD levels is adjudicated using the independent t-test. Specific antibody levels on treatment days 0, 3, and 6 showed significant differences (P < 0.05). Notably, significant differences were observed in comparisons such as severe recovery versus severe death groups on day 0 in IgG, mild versus severe recovery on day 3 in IgM, moderate versus severe recovery, severe recovery versus severe death, and mild versus severe recovery on day 3, as well as on day 6 in anti-S-RBD, and mild versus severe recovery on day 3 and day 6 in neutralization of SARS-CoV-2 (P < 0.05). These findings highlight the dynamic antibody responses during COVID-19 treatment and their relevance for clinical outcomes.
{"title":"Comparative Analysis of Nucleocapsid Antibody Levels, Neutralizing Antibodies, and Spike receptor-binding Domain in Severe Acute Respiratory Syndrome Coronavirus-2 Patients","authors":"Rinta Prasetiyanti, Munawaroh Fitriah, Yufi Aulia Azmi, Kevin Muliawan Soetanto, Marvin Castillo","doi":"10.4103/bhsj.bhsj_49_23","DOIUrl":"https://doi.org/10.4103/bhsj.bhsj_49_23","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 Serological testing for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is crucial for detecting antibodies generated in response to infection. Neutralizing antibodies (NAbs) target the receptor-binding domain (RBD) of the spike protein (S-RBD) and can exist in the forms of immunoglobulin G (IgG), IgM, and IgA. This study aimed to assess specific antibody responses to the nucleocapsid protein (N), S-RBD, and NAbs and correlate them with the clinical spectrum of coronavirus disease 2019 (COVID-19).\u0000 \u0000 \u0000 \u0000 A cross-sectional design was conducted at the Dr. Soetomo General Academic Hospital, Indonesia. We examined 48 patients with confirmed COVID-19 of varying severity (mild, moderate, severe, and critical) on treatment days 0, 3, and 6. We measured nucleocapsid antibodies, S-RBD, and neutralization using the chemiluminescence immunoassay method. The comparative analysis of IgM, IgG, NAB, and S-RBD levels is adjudicated using the independent t-test.\u0000 \u0000 \u0000 \u0000 Specific antibody levels on treatment days 0, 3, and 6 showed significant differences (P < 0.05). Notably, significant differences were observed in comparisons such as severe recovery versus severe death groups on day 0 in IgG, mild versus severe recovery on day 3 in IgM, moderate versus severe recovery, severe recovery versus severe death, and mild versus severe recovery on day 3, as well as on day 6 in anti-S-RBD, and mild versus severe recovery on day 3 and day 6 in neutralization of SARS-CoV-2 (P < 0.05).\u0000 \u0000 \u0000 \u0000 These findings highlight the dynamic antibody responses during COVID-19 treatment and their relevance for clinical outcomes.\u0000","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141010829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noi Maya Anggrita Sari, Lynda Hariani, Magda Rosalina Hutagalung, Al Farabi Lee Jaafar
ABSTRACT The midface is a complex anatomical area consisting of several areas. Although various factors protect the eye from trauma, such as orbital rim, blink reflex, elasticity of ocular structures, and protective eye movements by hands and head, ocular injuries remain common in patients with midface fractures, with an incidence rate of up to 90.6%. This study, the first of its kind conducted in Indonesia, aims to evaluate the characteristics and incidence of ocular injuries in midface fractures and to examine the relationship between the treatment. This observational analytic study employed a retrospective cohort design, utilizing medical record data of midface fracture patients. The independent variables included age, sex, cause, incidence, segment and severity of ocular injury, number of orbital wall involvements, and treatment options. The dependent variable was midface fracture patients. The Mann–Whitney and Fisher’s exact tests were employed to analyze the multivariate data. Individuals aged 32–37 were most commonly affected due to accidents, with the anterior ocular segment and maxilla bone frequently affected. There are no special factors that differentiate a person from experiencing an ocular injury in a midface fracture (P > 0.05). Ocular injuries correlated with orbital wall injury (P = 0.008) at least one discontinuity. Impaired functions included movement and visual disturbances, diplopia, and dystopia (P = 0.134). No significant relationship was found between age, sex, and cause of trauma with ocular injuries in patients with midface fractures. However, a significant relationship was observed between the amount of orbital rim involvement and at least one wall discontinuity occurring in midface fracture patients.
{"title":"Orbital Structure Involvement in Midface Fractures Impacting Ocular Injury at a Tertiary Hospital in Eastern Indonesia","authors":"Noi Maya Anggrita Sari, Lynda Hariani, Magda Rosalina Hutagalung, Al Farabi Lee Jaafar","doi":"10.4103/bhsj.bhsj_42_23","DOIUrl":"https://doi.org/10.4103/bhsj.bhsj_42_23","url":null,"abstract":"ABSTRACT\u0000 \u0000 \u0000 \u0000 The midface is a complex anatomical area consisting of several areas. Although various factors protect the eye from trauma, such as orbital rim, blink reflex, elasticity of ocular structures, and protective eye movements by hands and head, ocular injuries remain common in patients with midface fractures, with an incidence rate of up to 90.6%. This study, the first of its kind conducted in Indonesia, aims to evaluate the characteristics and incidence of ocular injuries in midface fractures and to examine the relationship between the treatment.\u0000 \u0000 \u0000 \u0000 This observational analytic study employed a retrospective cohort design, utilizing medical record data of midface fracture patients. The independent variables included age, sex, cause, incidence, segment and severity of ocular injury, number of orbital wall involvements, and treatment options. The dependent variable was midface fracture patients. The Mann–Whitney and Fisher’s exact tests were employed to analyze the multivariate data.\u0000 \u0000 \u0000 \u0000 Individuals aged 32–37 were most commonly affected due to accidents, with the anterior ocular segment and maxilla bone frequently affected. There are no special factors that differentiate a person from experiencing an ocular injury in a midface fracture (P > 0.05). Ocular injuries correlated with orbital wall injury (P = 0.008) at least one discontinuity. Impaired functions included movement and visual disturbances, diplopia, and dystopia (P = 0.134).\u0000 \u0000 \u0000 \u0000 No significant relationship was found between age, sex, and cause of trauma with ocular injuries in patients with midface fractures. However, a significant relationship was observed between the amount of orbital rim involvement and at least one wall discontinuity occurring in midface fracture patients.\u0000","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141010373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.20473/bhsj.v5i1.35280
Rizky Amalia Putri, Muhammad Noor Diansyah, Ami Ashariati, Siprianus Ugroseno Yudho Bintoro, Putu Niken Ayu Amrita, Merlyna Savitri, Pradana Zaky Romadhon
Bleeding is one of the most common complaints when coming to the hospital which can be mild to life-threatening. The balance of the impaired hemostasis system allows for abnormal bleeding such as hemorrhagic diathesis. Balance between blood clotting and bleeding is always maintained in the body under normal physiology. The coagulation system stops existing bleeding with vasoconstricts of blood vessels and the formation of early platelet plugs, this blockage is strengthened by the presence of a cascade of coagulations to form stable and sturdy blockages. Once bleeding has stopped, the fibrinolytic pathway is initiated to dissolve the blood clot to restore normal blood flow. balance the coagulants, fibrinolytic and inhibitor systems, creating a perfect physiological balance. Hemostatic imbalance is a global problem that can lead to thrombosis or hemorrhage.
{"title":"Diathesis Hemorrhagic, Coagulation and Fibrinolytic System","authors":"Rizky Amalia Putri, Muhammad Noor Diansyah, Ami Ashariati, Siprianus Ugroseno Yudho Bintoro, Putu Niken Ayu Amrita, Merlyna Savitri, Pradana Zaky Romadhon","doi":"10.20473/bhsj.v5i1.35280","DOIUrl":"https://doi.org/10.20473/bhsj.v5i1.35280","url":null,"abstract":"Bleeding is one of the most common complaints when coming to the hospital which can be mild to life-threatening. The balance of the impaired hemostasis system allows for abnormal bleeding such as hemorrhagic diathesis. Balance between blood clotting and bleeding is always maintained in the body under normal physiology. The coagulation system stops existing bleeding with vasoconstricts of blood vessels and the formation of early platelet plugs, this blockage is strengthened by the presence of a cascade of coagulations to form stable and sturdy blockages. Once bleeding has stopped, the fibrinolytic pathway is initiated to dissolve the blood clot to restore normal blood flow. balance the coagulants, fibrinolytic and inhibitor systems, creating a perfect physiological balance. Hemostatic imbalance is a global problem that can lead to thrombosis or hemorrhage.","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74354126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.20473/bhsj.v5i1.34442
R. Wibowo, B. Prasetyo, Widati Fatmaningrum, Ahmad Hadi Modi
Introduction: Birth weight is often used to evaluate a newborn baby’s health. Low birth weight (LBW) is one of the leading causes of neonatal mortality. Mothers under the age of 20 (early pregnancy) are at a greater risk of affecting the weight and nutritional status of the unborn child. This research aimed to determine the relationship between early pregnancy and LBW. Methods: This was a cross-sectional study of women and newborns at Airlangga University academic hospital from January through December of 2017. Data on characteristics such as maternal age and LBW were collected. The correlation between early pregnancy and infant weight was determined using the Chi-square test, with a significance level of p<0.05. Results: We conducted research on 210 pairs of mothers and their infants. 64 (30.48%) of the pregnancies occurred in women under the age of 20; 61 (29.55%) were born LBW (weighing less than 2,500 grams). Early age pregnancy was linked to a higher proportion of LBW infants (p=0.001). Research indicated that the risk of LBW was more significant in the early groups of pregnancy.Conclusion: This research establishes a relationship between early age pregnancy and LBW. The findings may aid in identifying vulnerable mothers in need of further assistance and personalized treatments.
{"title":"Correlation Between Early Age Pregnancy with Low Body Weight (LBW) Newborn at Universitas Airlangga Academic Hospital, Surabaya, Indonesia","authors":"R. Wibowo, B. Prasetyo, Widati Fatmaningrum, Ahmad Hadi Modi","doi":"10.20473/bhsj.v5i1.34442","DOIUrl":"https://doi.org/10.20473/bhsj.v5i1.34442","url":null,"abstract":"Introduction: Birth weight is often used to evaluate a newborn baby’s health. Low birth weight (LBW) is one of the leading causes of neonatal mortality. Mothers under the age of 20 (early pregnancy) are at a greater risk of affecting the weight and nutritional status of the unborn child. This research aimed to determine the relationship between early pregnancy and LBW. Methods: This was a cross-sectional study of women and newborns at Airlangga University academic hospital from January through December of 2017. Data on characteristics such as maternal age and LBW were collected. The correlation between early pregnancy and infant weight was determined using the Chi-square test, with a significance level of p<0.05. Results: We conducted research on 210 pairs of mothers and their infants. 64 (30.48%) of the pregnancies occurred in women under the age of 20; 61 (29.55%) were born LBW (weighing less than 2,500 grams). Early age pregnancy was linked to a higher proportion of LBW infants (p=0.001). Research indicated that the risk of LBW was more significant in the early groups of pregnancy.Conclusion: This research establishes a relationship between early age pregnancy and LBW. The findings may aid in identifying vulnerable mothers in need of further assistance and personalized treatments.","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90324920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.20473/bhsj.v5i1.35279
Erlyta Dian Puspitarini, Prijambodo Prijambodo
Introduction: Colorectal cancer is the third most malignant and the fourth-largest cause of death in the world, one of which is caused by cachexia cancer. Sarcopenia is the main diagnostic criterion for cachexia. The inflammatory response, one of the markers of which is C-Reactive Protein (CRP), is also involved in the occurrence of sarcopenia associated with cachexia cancer. This study aimed to determine the occurrence of sarcopenia and its relationship with CRP preoperative colorectal cancer patients. Methods: This type of observational retrospective analytic study assessed sarcopenia based on the Psoas muscle index (IOP) on preoperative CT scan images and CRP levels measured by an integrated automatic tool Dimension RxL Max with Flex liquid reagent preoperative CRP range (RCRP). Results: Sarcopenia occurs in male colorectal cancer patients with a p-value of 0.001<0.05 (5%) with an IOP mean value of 26.75 m2, while in female patients the IOP mean value was 21.33 m2, it does not occur sarcopenia with p-value 0.583>0.05 (5%). There was an increase in CRP in 62.5% of colorectal cancer patients, but the relationship between IOP values and CRP values of colorectal cancer patients was still categorized as weak with p value of -0.387 in men and -0.046 in women with α=0.05. Conclusion: There was a nonsignificant relationship between sarcopenia and CRP levels in colorectal cancer patients. Further prospective studies are required to consider the indicators of sarcopenia besides the decrease in IOP and identification of confounding factors known to be associated with CRP, muscle strength, and muscle mass.
{"title":"Relationship between Sarcopenia in Abdomen CT Scan Results with C-Reactive Protein Level in Colorectal Cancer Patients at Dr. Soetomo General Academic Hospital Surabaya","authors":"Erlyta Dian Puspitarini, Prijambodo Prijambodo","doi":"10.20473/bhsj.v5i1.35279","DOIUrl":"https://doi.org/10.20473/bhsj.v5i1.35279","url":null,"abstract":"Introduction: Colorectal cancer is the third most malignant and the fourth-largest cause of death in the world, one of which is caused by cachexia cancer. Sarcopenia is the main diagnostic criterion for cachexia. The inflammatory response, one of the markers of which is C-Reactive Protein (CRP), is also involved in the occurrence of sarcopenia associated with cachexia cancer. This study aimed to determine the occurrence of sarcopenia and its relationship with CRP preoperative colorectal cancer patients. Methods: This type of observational retrospective analytic study assessed sarcopenia based on the Psoas muscle index (IOP) on preoperative CT scan images and CRP levels measured by an integrated automatic tool Dimension RxL Max with Flex liquid reagent preoperative CRP range (RCRP). Results: Sarcopenia occurs in male colorectal cancer patients with a p-value of 0.001<0.05 (5%) with an IOP mean value of 26.75 m2, while in female patients the IOP mean value was 21.33 m2, it does not occur sarcopenia with p-value 0.583>0.05 (5%). There was an increase in CRP in 62.5% of colorectal cancer patients, but the relationship between IOP values and CRP values of colorectal cancer patients was still categorized as weak with p value of -0.387 in men and -0.046 in women with α=0.05. Conclusion: There was a nonsignificant relationship between sarcopenia and CRP levels in colorectal cancer patients. Further prospective studies are required to consider the indicators of sarcopenia besides the decrease in IOP and identification of confounding factors known to be associated with CRP, muscle strength, and muscle mass.","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81368721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.20473/bhsj.v5i1.32147
Mega Memory Rahasa Putra, Kurnia Penta Seputra
Introduction: Prostate cancer is a leading global cause of increased mortality and morbidity in men which can be complicated by castrate-resistant prostate cancer (CRPC). Pharmacological therapy by inhibiting the androgen receptor (AR) can inhibit prostate cancer progression. Tea leaves (Camellia sinensis) are believed to inhibit the prostate cancer progression but the mechanism is still unknown. Therefore, research on the mechanism by in silico study is needed with the AR as target protein.Methods: The effectivity of tea leaves’ active compound to inhibit androgen receptor was evaluated by docking server with abiraterone acetate as a control. The tea leaves' active compounds consist of catechin, epicatechin, epigallocatechin gallate, epigallocatechin, gallate epicatechin, gallocatechin gallate, and gallocatechinResults: The result showed that epicatechin, epigallocatechin, and gallocatechin have lower free binding energy (ΔG) and high amino acid residue similarity on AR compared with abiraterone acetate. But, it has lower surface interaction compared with abiraterone acetate. Conclusion: Epicatechin, epigallocatechin, and gallocatechin are predicted to have potential as alternative therapy in CRPC with AR Inhibition.
{"title":"The Potential of Cammelia sinensis (Tea Leaves) Active Compound as Alternative Therapy on castrate-resistant prostate cancer (CRPC) with Androgen Receptor Inhibition: In Silico Study","authors":"Mega Memory Rahasa Putra, Kurnia Penta Seputra","doi":"10.20473/bhsj.v5i1.32147","DOIUrl":"https://doi.org/10.20473/bhsj.v5i1.32147","url":null,"abstract":"Introduction: Prostate cancer is a leading global cause of increased mortality and morbidity in men which can be complicated by castrate-resistant prostate cancer (CRPC). Pharmacological therapy by inhibiting the androgen receptor (AR) can inhibit prostate cancer progression. Tea leaves (Camellia sinensis) are believed to inhibit the prostate cancer progression but the mechanism is still unknown. Therefore, research on the mechanism by in silico study is needed with the AR as target protein.Methods: The effectivity of tea leaves’ active compound to inhibit androgen receptor was evaluated by docking server with abiraterone acetate as a control. The tea leaves' active compounds consist of catechin, epicatechin, epigallocatechin gallate, epigallocatechin, gallate epicatechin, gallocatechin gallate, and gallocatechinResults: The result showed that epicatechin, epigallocatechin, and gallocatechin have lower free binding energy (ΔG) and high amino acid residue similarity on AR compared with abiraterone acetate. But, it has lower surface interaction compared with abiraterone acetate. Conclusion: Epicatechin, epigallocatechin, and gallocatechin are predicted to have potential as alternative therapy in CRPC with AR Inhibition.","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89541716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.20473/bhsj.v5i1.34827
Khen Mikhael, D. Husada, Pudji Lestari
Introduction: Dengue virus infection is caused by the dengue virus and transmitted through the bites of infected Aedes aegypti or Aedes albopictus mosquitoes. The spectrum of clinical manifestations is varied from asymptomatic, undifferentiated fever, dengue fever, dengue hemorrhagic fever, dengue shock syndrome, to expanded dengue syndrome. Data from 2016 in Indonesia revealed that dengue virus infection is common in the population, with a total of 333.821 cases of dengue hemorrhagic fever. Methods: This was a descriptive study with a cross-sectional design. The subjects in this study were children under 1-year-old, diagnosed with dengue virus infection, and had a complication. The number of subjects used is 60 infants. Results: Infection of dengue virus in infants is often found in the 7-9 month age group (36,6%). Clinical manifestations found were fever (100%), vomiting (62%), diarrhea (43%), petechiae (22%), bleeding (12%), and irritable (5%). Thrombocytopenia and leukopenia were found in 93,3% and 26,6% of infants respectively. Complications were found in the form of pleural effusion (66%), hypoalbuminemia (62%), hyponatremia (51%), liver involvement (49%), hypocalcemia (43%), hypokalemia (23%), bleeding (21%), brain involvement (21%), kidney involvement (13%), and ascites (11%).Conclusion: The most common clinical manifestations are fever, diarrhea, and vomiting, while the most common complications are pleural effusion, hypoalbuminemia, hyponatremia, liver involvement, and hypocalcemia.
{"title":"Profile of Dengue Fever Complication in Infant at Tertiary Referral Hospital in East Java, Indonesia","authors":"Khen Mikhael, D. Husada, Pudji Lestari","doi":"10.20473/bhsj.v5i1.34827","DOIUrl":"https://doi.org/10.20473/bhsj.v5i1.34827","url":null,"abstract":"Introduction: Dengue virus infection is caused by the dengue virus and transmitted through the bites of infected Aedes aegypti or Aedes albopictus mosquitoes. The spectrum of clinical manifestations is varied from asymptomatic, undifferentiated fever, dengue fever, dengue hemorrhagic fever, dengue shock syndrome, to expanded dengue syndrome. Data from 2016 in Indonesia revealed that dengue virus infection is common in the population, with a total of 333.821 cases of dengue hemorrhagic fever. Methods: This was a descriptive study with a cross-sectional design. The subjects in this study were children under 1-year-old, diagnosed with dengue virus infection, and had a complication. The number of subjects used is 60 infants. Results: Infection of dengue virus in infants is often found in the 7-9 month age group (36,6%). Clinical manifestations found were fever (100%), vomiting (62%), diarrhea (43%), petechiae (22%), bleeding (12%), and irritable (5%). Thrombocytopenia and leukopenia were found in 93,3% and 26,6% of infants respectively. Complications were found in the form of pleural effusion (66%), hypoalbuminemia (62%), hyponatremia (51%), liver involvement (49%), hypocalcemia (43%), hypokalemia (23%), bleeding (21%), brain involvement (21%), kidney involvement (13%), and ascites (11%).Conclusion: The most common clinical manifestations are fever, diarrhea, and vomiting, while the most common complications are pleural effusion, hypoalbuminemia, hyponatremia, liver involvement, and hypocalcemia.","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90726941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.20473/bhsj.v5i1.31880
Archangela Olivia Eugenia, A. F. Sani, H. Susanto, J. H. Prajitno
Introduction: The prevalence of type 2 Diabetes Mellitus (T2DM) in Indonesia is increasing and is known to cause several complications related to the patient’s glycemic control. A chronic hyperglycemic state will lead to microvascular injury of the brain resulting in cognitive impairment as one of the complications of T2DM. Therefore, our present study observed the correlation between glycemic control and cognitive impairment in patients with T2DM.Methods: This was an observational study with a cross-sectional design of T2DM patients in endocrine outpatient clinics of Dr. Soetomo General Academic Hospital from October 2020 to March 2021. The subjects were recruited consecutively then categorized into groups with controlled (HbA1c < 7%) and uncontrolled (HbA1c ≥ 7%) blood glucose. The cognitive function was evaluated using the AD8 informant-based questionnaire.Results: A total of 43 adult T2DM patients aged < 65 years were recruited. The incidence of cognitive impairment was not significantly different (p=0.127) between controlled and uncontrolled blood glucose groups. However, HbA1c levels were positively and significantly correlated with AD8 scores (p=0.031, R=0.330). Moreover, the duration of T2DM was found to significantly affect cognitive abnormalities in these patients. (p=0.021).Conclusion: Poor glycemic control in T2DM patients increased the risk of developing reduced cognitive function.
{"title":"Poor Glycemic Control is Correlated with Reduced Cognitive Function in Type 2 Diabetes Mellitus Patients","authors":"Archangela Olivia Eugenia, A. F. Sani, H. Susanto, J. H. Prajitno","doi":"10.20473/bhsj.v5i1.31880","DOIUrl":"https://doi.org/10.20473/bhsj.v5i1.31880","url":null,"abstract":"Introduction: The prevalence of type 2 Diabetes Mellitus (T2DM) in Indonesia is increasing and is known to cause several complications related to the patient’s glycemic control. A chronic hyperglycemic state will lead to microvascular injury of the brain resulting in cognitive impairment as one of the complications of T2DM. Therefore, our present study observed the correlation between glycemic control and cognitive impairment in patients with T2DM.Methods: This was an observational study with a cross-sectional design of T2DM patients in endocrine outpatient clinics of Dr. Soetomo General Academic Hospital from October 2020 to March 2021. The subjects were recruited consecutively then categorized into groups with controlled (HbA1c < 7%) and uncontrolled (HbA1c ≥ 7%) blood glucose. The cognitive function was evaluated using the AD8 informant-based questionnaire.Results: A total of 43 adult T2DM patients aged < 65 years were recruited. The incidence of cognitive impairment was not significantly different (p=0.127) between controlled and uncontrolled blood glucose groups. However, HbA1c levels were positively and significantly correlated with AD8 scores (p=0.031, R=0.330). Moreover, the duration of T2DM was found to significantly affect cognitive abnormalities in these patients. (p=0.021).Conclusion: Poor glycemic control in T2DM patients increased the risk of developing reduced cognitive function.","PeriodicalId":9324,"journal":{"name":"Biomolecular and Health Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77075921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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