Pub Date : 2019-10-01DOI: 10.29328/journal.jpsp.1001037
MH Abd El-Aziz, H. Younes
Bruening and Agrawal, [3]. Little work has been done on Cowpea mosaic comovirus (CPMV) in Egypt. It is one of the most commonly reported virus diseases of cowpea, which causes mosaic and decreases leaf area and lower production [4]. Symptoms induced by CPMV vary from light green mottle to distinct yellow mosaic, distortion of leaf, and premature death of the plant [5]. In our research detection of CPMV infecting cowpea in northern Egypt was based mainly on serological diagnosis and RT-PCR. The aims of this work were detection and isolation of CPMV affecting cowpea plants. Viral characterization included host range and symptomology, modes of transmission, sap infectivity tests, serological tests and nucleotide sequencing. Detection of CPMV isolate1 in various plant organs such as loral parts, pods, seed and seed parts showed positive results. Comparative studies for detection of CPMV by TBIA, DBIA, indirect ELISA and biological assay in infected plants were conducted after different periods of inoculation, and in serial dilutions of infected plant sap. Possibility of using alternative solid carriers instead of nitrocellulose membranes in TBIA and DBIA tests was determined using two faces of the solid carriers in TBIA test. Partial sequences of the virus isolates was registered in gene bank.
{"title":"Serological and molecular characterization of two seed born cowpea mosaic Comovirus isolates affecting cowpea plants (Vigna unguiculata L.) in northern","authors":"MH Abd El-Aziz, H. Younes","doi":"10.29328/journal.jpsp.1001037","DOIUrl":"https://doi.org/10.29328/journal.jpsp.1001037","url":null,"abstract":"Bruening and Agrawal, [3]. Little work has been done on Cowpea mosaic comovirus (CPMV) in Egypt. It is one of the most commonly reported virus diseases of cowpea, which causes mosaic and decreases leaf area and lower production [4]. Symptoms induced by CPMV vary from light green mottle to distinct yellow mosaic, distortion of leaf, and premature death of the plant [5]. In our research detection of CPMV infecting cowpea in northern Egypt was based mainly on serological diagnosis and RT-PCR. The aims of this work were detection and isolation of CPMV affecting cowpea plants. Viral characterization included host range and symptomology, modes of transmission, sap infectivity tests, serological tests and nucleotide sequencing. Detection of CPMV isolate1 in various plant organs such as loral parts, pods, seed and seed parts showed positive results. Comparative studies for detection of CPMV by TBIA, DBIA, indirect ELISA and biological assay in infected plants were conducted after different periods of inoculation, and in serial dilutions of infected plant sap. Possibility of using alternative solid carriers instead of nitrocellulose membranes in TBIA and DBIA tests was determined using two faces of the solid carriers in TBIA test. Partial sequences of the virus isolates was registered in gene bank.","PeriodicalId":93470,"journal":{"name":"Journal of plant science and phytopathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43914547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-13DOI: 10.29328/journal.jpsp.1001036
M. Khalil, Dalia M Darwesh
Plant parasitic nematodes (PPNs) considered a threat to crops production and are responsible for about US $173 billion annual losses in agriculture world [1]. Sometimes, PPNs are co-operating with other plant pathogens such as bacteria and fungi and cause complex diseases. Nematodes can damage all parts of their host plants, although according to their life style, individual species target the roots, stems, leaves, lowers, or seeds. There are about 31% of known species of nematodes are parasites of vertebrates [2], while 16% are parasites of plants, mostly soil-borne root pathogens [3]. Approximately 2.4% species of those parasites on plants are responsible for economically important phytoparasites of crops.
{"title":"Avermectins: The promising solution to control plant parasitic nematodes","authors":"M. Khalil, Dalia M Darwesh","doi":"10.29328/journal.jpsp.1001036","DOIUrl":"https://doi.org/10.29328/journal.jpsp.1001036","url":null,"abstract":"Plant parasitic nematodes (PPNs) considered a threat to crops production and are responsible for about US $173 billion annual losses in agriculture world [1]. Sometimes, PPNs are co-operating with other plant pathogens such as bacteria and fungi and cause complex diseases. Nematodes can damage all parts of their host plants, although according to their life style, individual species target the roots, stems, leaves, lowers, or seeds. There are about 31% of known species of nematodes are parasites of vertebrates [2], while 16% are parasites of plants, mostly soil-borne root pathogens [3]. Approximately 2.4% species of those parasites on plants are responsible for economically important phytoparasites of crops.","PeriodicalId":93470,"journal":{"name":"Journal of plant science and phytopathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44532653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-30DOI: 10.29328/JOURNAL.JPSP.1001034
Y. Choong, Nor Syaidatul Akmal Mohd Yousof, J. Jamal, Mohd Isa Wasiman
Hibiscus sabdariffa, a famous herbal plant in Malaysia [1], belongs to the family Malvaceae and is locally known as “Roselle” [2]. It is an important annual crop grown successfully in tropical and sub-tropical climates [3]. Its leshy calyx (sepals) surrounding the fruit (capsules) have been used to produce a variety of beverages and commercial products. The therapeutic anti-obesity usage of H. sabdariffa has been well studied [4]. In fact, many signi icant evidences of H. sabdariffa in lowering deposition of fat in adipose tissue [5], have motivated the investigation. A study by Ojeda et Abstract
{"title":"Determination of anthocyanin content in two varieties of Hibiscus Sabdariffa from Selangor, Malaysia using a combination of chromatography and spectroscopy","authors":"Y. Choong, Nor Syaidatul Akmal Mohd Yousof, J. Jamal, Mohd Isa Wasiman","doi":"10.29328/JOURNAL.JPSP.1001034","DOIUrl":"https://doi.org/10.29328/JOURNAL.JPSP.1001034","url":null,"abstract":"Hibiscus sabdariffa, a famous herbal plant in Malaysia [1], belongs to the family Malvaceae and is locally known as “Roselle” [2]. It is an important annual crop grown successfully in tropical and sub-tropical climates [3]. Its leshy calyx (sepals) surrounding the fruit (capsules) have been used to produce a variety of beverages and commercial products. The therapeutic anti-obesity usage of H. sabdariffa has been well studied [4]. In fact, many signi icant evidences of H. sabdariffa in lowering deposition of fat in adipose tissue [5], have motivated the investigation. A study by Ojeda et Abstract","PeriodicalId":93470,"journal":{"name":"Journal of plant science and phytopathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44838143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-19DOI: 10.29328/JOURNAL.JPSP.1001033
N. Kazi, I. B. Chimbekujwo, S. Anjili
Pumpkin, one of the common names for lowering plants that belong to the Curcubitaceae family with four genera which include Curcubita maxima, Curcubita pepo, Curcubita moschata and Cucurbita mixta. They are characterized by spreading vines with showy yellow-orange lowers, large lobed leaves, long twisting tendrils and thick shell which contain the seeds and pulp [1]. They are grown all around the world for a variety of reasons ranging from agricultural purposes (such as animal feed) to commercial and ornamental purposes [2].
{"title":"Causal agents of Post-harvest Rot of Pumpkin (Cucurbita pepo L.) and their control using Indigenous Practices in Hong, Adamawa State","authors":"N. Kazi, I. B. Chimbekujwo, S. Anjili","doi":"10.29328/JOURNAL.JPSP.1001033","DOIUrl":"https://doi.org/10.29328/JOURNAL.JPSP.1001033","url":null,"abstract":"Pumpkin, one of the common names for lowering plants that belong to the Curcubitaceae family with four genera which include Curcubita maxima, Curcubita pepo, Curcubita moschata and Cucurbita mixta. They are characterized by spreading vines with showy yellow-orange lowers, large lobed leaves, long twisting tendrils and thick shell which contain the seeds and pulp [1]. They are grown all around the world for a variety of reasons ranging from agricultural purposes (such as animal feed) to commercial and ornamental purposes [2].","PeriodicalId":93470,"journal":{"name":"Journal of plant science and phytopathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47423321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-16DOI: 10.29328/JOURNAL.JPSP.1001032
Yilin Shen, Wei-Qi Nian
The plant lowering process represents the transition from vegetative to productive growth, and is controlled by complicated innergenetic regulation and the external environment. Flowering is closely related to the heading date and maturity crops, and has been studied for hundreds of years [1]. Currently, studies on Arabidopsis thaliana have revealed ive pathways, the vernalization, photoperiod, gibberellin, autonomous, and endogenous pathways [1]. Initially, it was discovered that day length, namely photoperiod, could be perceived by higher plants and impacted their lowering. Over these years, different models have been put forward to explain the relationship between lowering and photoperiod [2,3]. Recently, a growing number of genes have been characterized as effectors of the natural condition or internal regulatory network, which has enhanced our knowledge of the regulators mediating the lowering pathway and plant circadian clock.
{"title":"Cloning and Characterization of a Pseudo-Response Regulator 7 (PRR7) Gene from Medicago Sativa Involved In Regulating the Circadian Clock","authors":"Yilin Shen, Wei-Qi Nian","doi":"10.29328/JOURNAL.JPSP.1001032","DOIUrl":"https://doi.org/10.29328/JOURNAL.JPSP.1001032","url":null,"abstract":"The plant lowering process represents the transition from vegetative to productive growth, and is controlled by complicated innergenetic regulation and the external environment. Flowering is closely related to the heading date and maturity crops, and has been studied for hundreds of years [1]. Currently, studies on Arabidopsis thaliana have revealed ive pathways, the vernalization, photoperiod, gibberellin, autonomous, and endogenous pathways [1]. Initially, it was discovered that day length, namely photoperiod, could be perceived by higher plants and impacted their lowering. Over these years, different models have been put forward to explain the relationship between lowering and photoperiod [2,3]. Recently, a growing number of genes have been characterized as effectors of the natural condition or internal regulatory network, which has enhanced our knowledge of the regulators mediating the lowering pathway and plant circadian clock.","PeriodicalId":93470,"journal":{"name":"Journal of plant science and phytopathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47875848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}