首页 > 最新文献

ACS agricultural science & technology最新文献

英文 中文
Inventory and Review of Existing Agricultural Best Management Practices for Pesticides in the United States 美国现有农药农业最佳管理实践的盘点和审查
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-19 DOI: 10.1021/acsagscitech.3c00588
Andy Jacobson*, Nick Guth, Zechariah Stone, Dean Desmarteau and Richard Brain, 

To reduce pesticide exposure to nontarget organisms in the United States (US), mitigations are being proposed a priori for regulatory compliance. Consequently, agricultural best management practices (BMPs) for pesticide runoff, proposed in the Environmental Protection Agency’s Endangered Species Act (ESA) workplan [USEPA. ESA Workplan Update: Nontarget Species Mitigation for RegistrationReview and Other FIFRA Actions, 2022. https://www.epa.gov/system/files/documents/2022-11/esa-workplan-update.pdf (accessed Feb, 2023)], were inventoried and their feasibility was evaluated. For mitigation “menus” to be successful, they must be comprehensive and include options for a variety of environmental conditions. Based on this inventory, the most adopted practices by landowners were soil cover and erosion control practices due to their widespread applicability to most cropland. In contrast, the least adopted practices were water management and vegetative buffer practices due to increased costs and land requirements. This work can support the refinement of the pesticide risk assessment process, including future mitigation options, and assist landowners in selecting the most feasible BMPs for their individual operation in compliance with the ESA.

在美国,为了减少非目标生物接触农药的机会,正在事先提出缓解措施,以符合法规要求。因此,环境保护局的《濒危物种法》(ESA)工作计划[USEPA.https://www.epa.gov/system/files/documents/2022-11/esa-workplan-update.pdf (accessed Feb, 2023)],对其可行性进行了评估。缓解 "菜单 "要想取得成功,就必须全面,并包括各种环境条件下的备选方案。根据这份清单,土地所有者采用最多的措施是土壤覆盖和侵蚀控制措施,因为它们广泛适用于大多数耕地。相比之下,采用最少的是水管理和植被缓冲措施,原因是成本和土地需求增加。这项工作可以支持农药风险评估过程的完善,包括未来的缓解方案,并帮助土地所有者选择最可行的 BMP,以符合 ESA 的要求。
{"title":"Inventory and Review of Existing Agricultural Best Management Practices for Pesticides in the United States","authors":"Andy Jacobson*,&nbsp;Nick Guth,&nbsp;Zechariah Stone,&nbsp;Dean Desmarteau and Richard Brain,&nbsp;","doi":"10.1021/acsagscitech.3c00588","DOIUrl":"10.1021/acsagscitech.3c00588","url":null,"abstract":"<p >To reduce pesticide exposure to nontarget organisms in the United States (US), mitigations are being proposed a priori for regulatory compliance. Consequently, agricultural best management practices (BMPs) for pesticide runoff, proposed in the Environmental Protection Agency’s Endangered Species Act (ESA) workplan [<contrib-group>USEPA</contrib-group>. ESA Workplan Update: Nontarget Species Mitigation for RegistrationReview and Other FIFRA Actions, <span>2022</span>. https://www.epa.gov/system/files/documents/2022-11/esa-workplan-update.pdf (accessed Feb, 2023)], were inventoried and their feasibility was evaluated. For mitigation “menus” to be successful, they must be comprehensive and include options for a variety of environmental conditions. Based on this inventory, the most adopted practices by landowners were soil cover and erosion control practices due to their widespread applicability to most cropland. In contrast, the least adopted practices were water management and vegetative buffer practices due to increased costs and land requirements. This work can support the refinement of the pesticide risk assessment process, including future mitigation options, and assist landowners in selecting the most feasible BMPs for their individual operation in compliance with the ESA.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 5","pages":"567–579"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.3c00588","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140683656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Synthesis and Insecticidal Activity Evaluation of Potential Insecticidal Natural Product Borrelidin Analogs Toward New Pesticides” 对 "潜在杀虫天然产品 Borrelidin 类似物的合成和杀虫活性评估,以开发新杀虫剂 "的更正
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-12 DOI: 10.1021/acsagscitech.4c00152
Naozumi Kondo, Aoi Kimishima, Serino Maruyama, Sota Honma, Atsuka Nishitomi, Kazunari Sakai, Masako Honsho, Yoshihiro Watanabe, Masato Iwatsuki and Yukihiro Asami*, 
{"title":"Correction to “Synthesis and Insecticidal Activity Evaluation of Potential Insecticidal Natural Product Borrelidin Analogs Toward New Pesticides”","authors":"Naozumi Kondo,&nbsp;Aoi Kimishima,&nbsp;Serino Maruyama,&nbsp;Sota Honma,&nbsp;Atsuka Nishitomi,&nbsp;Kazunari Sakai,&nbsp;Masako Honsho,&nbsp;Yoshihiro Watanabe,&nbsp;Masato Iwatsuki and Yukihiro Asami*,&nbsp;","doi":"10.1021/acsagscitech.4c00152","DOIUrl":"10.1021/acsagscitech.4c00152","url":null,"abstract":"","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 5","pages":"626–627"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140709772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insight into Mechanical Extraction Mechanisms of Natural Rubber Extraction from Taraxacum kok-saghyz 洞察从蒲公英中提取天然橡胶的机械提取机制
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-11 DOI: 10.1021/acsagscitech.3c00513
Clemens Fichtner*, Hazem Fathelbab and Philipp Weißgraeber, 

The only commercially relevant source for natural rubber (NR) is the Para rubber tree, Hevea brasiliensis. The need to find new sources of NR is increasing rapidly due to challenges from climate change and environmental impacts. NR from Taraxacum kok-saghyz (TKS) is a promising alternative. To compete with the pricing of NR from the rubber tree, an economically viable extraction system with a high output of NR is needed. The mechanisms involved in the separation of rubber from other plant constituents and potential rubber agglomeration during mechanical extraction processes are described through experimental results but not as extraction models. To analyze these mechanisms, two different wet-mill extraction system setups are investigated in this study: single-stage extraction and two-stage extraction with premilling. Two extraction models for mechanical rubber extraction mechanisms are identified and described: (i) the Model of Rolling Shear Mechanism with mild extraction behavior and rubber agglomeration, and (ii) the Model of Abrasion Shear Mechanism, which limits the rubber yield and promotes fast rubber agglomeration. The insights into the mechanisms are described and discussed through sieve analysis, rubber yield, dirt content, microscopic analyses, and viscoelastic analyses of the extracted rubber.

天然橡胶(NR)的唯一商业相关来源是顺丁橡胶树(Hevea brasiliensis)。由于气候变化和环境影响带来的挑战,寻找天然橡胶新来源的需求正在迅速增加。从蒲公英(Taraxacum kok-saghyz,TKS)中提取的天然橡胶是一种很有前景的替代品。为了在价格上与从橡胶树中提取的 NR 竞争,需要一种经济可行且 NR 产量高的提取系统。在机械萃取过程中,橡胶与其他植物成分的分离机制以及潜在的橡胶结块都是通过实验结果而非萃取模型来描述的。为了分析这些机制,本研究调查了两种不同的湿法碾磨萃取系统设置:单级萃取和带预磨的两级萃取。研究确定并描述了两种机械橡胶萃取机制的萃取模型:(i) 轧辊剪切机制模型,该模型具有温和的萃取行为和橡胶结块;(ii) 磨擦剪切机制模型,该模型限制橡胶产量并促进橡胶快速结块。通过筛分分析、橡胶产量、污垢含量、微观分析和提取橡胶的粘弹性分析,对这些机制进行了描述和讨论。
{"title":"Insight into Mechanical Extraction Mechanisms of Natural Rubber Extraction from Taraxacum kok-saghyz","authors":"Clemens Fichtner*,&nbsp;Hazem Fathelbab and Philipp Weißgraeber,&nbsp;","doi":"10.1021/acsagscitech.3c00513","DOIUrl":"10.1021/acsagscitech.3c00513","url":null,"abstract":"<p >The only commercially relevant source for natural rubber (NR) is the Para rubber tree, <i>Hevea brasiliensis</i>. The need to find new sources of NR is increasing rapidly due to challenges from climate change and environmental impacts. NR from <i>Taraxacum kok-saghyz</i> (TKS) is a promising alternative. To compete with the pricing of NR from the rubber tree, an economically viable extraction system with a high output of NR is needed. The mechanisms involved in the separation of rubber from other plant constituents and potential rubber agglomeration during mechanical extraction processes are described through experimental results but not as extraction models. To analyze these mechanisms, two different wet-mill extraction system setups are investigated in this study: single-stage extraction and two-stage extraction with premilling. Two extraction models for mechanical rubber extraction mechanisms are identified and described: (i) the Model of Rolling Shear Mechanism with mild extraction behavior and rubber agglomeration, and (ii) the Model of Abrasion Shear Mechanism, which limits the rubber yield and promotes fast rubber agglomeration. The insights into the mechanisms are described and discussed through sieve analysis, rubber yield, dirt content, microscopic analyses, and viscoelastic analyses of the extracted rubber.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 5","pages":"524–534"},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140716506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extraction and Analysis of Microplastics in the Soil of Diamond City, Surat (Gujarat, India): Ecological Risk, Pollution Indices, and Greenness Evaluation 苏拉特钻石城(印度古吉拉特邦)土壤中微塑料的提取与分析:生态风险、污染指数和绿色评价
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-09 DOI: 10.1021/acsagscitech.4c00140
Pinal S. Bhavsar, Bhautik V. Chovatiya, Sumit B. Kamble and Anil H. Gore*, 

This study examines microplastics (MPs) for the first time in the soil of Diamond City, Surat (Gujarat, India) in terms of their abundance, distribution, chemical composition, and ecological risk assessment. A stereomicroscope and Fourier transform infrared spectroscopy were used to measure the physical and chemical properties of the MPs. The outcome showed the omnipresence of MPs in Surat city’s soil, with an average abundance of 103.2 ± 57.28 particles 100 g–1. Over 90% of all MPs were made of polyethylene (70.19%), polypropylene (17.53%), and poly(ethylene terephthalate) (6.92%) polymers. The white/transparent-colored MPs having a size of about 2–5 mm are predominantly available. The principal component analysis (PCA) results indicate that the discovered MPs in the soil samples are mostly represented by fragments and fibers, which account for 52.90% of the total variance in the data set. Additionally, in the current research, the ecological risk assessment of MPs in soil, the pollution load index (PLI), the polymer hazard index (PHI), and the ecological risk index (ERI) of MPs were studied. The PLI values greater than 1 were observed, indicating that all sampling sites were polluted with MPs. Based on the abundance, type of polymer, and toxicity of MPs found in the soil sample, an evaluation concluded that there is a moderate to medium ecological risk associated with MPs polluting the city soil. The greatest PHI value in this study is 239.69 (risk category: medium), while the lowest PHI value is 8.69 (risk category: low). The greenness profile of the method was assessed using the software-based analytical greenness metric (AGREE) tool. The developed approach is partially green, as shown by an AGREE metric score of 0.53. The present investigations emphasize the critical importance of taking action in metropolitan areas such as Surat to mitigate MP pollution and improve waste management practices for a greener and cleaner ecosystem in the future. These findings will inspire the creation of ecofriendly alternatives crucial for reducing plastic consumption, safeguarding ecosystems, and mitigating MPs’ impact on the soil quality.

本研究首次对苏拉特钻石城(印度古吉拉特邦)土壤中的微塑料(MPs)的丰度、分布、化学成分和生态风险评估进行了研究。研究使用了体视显微镜和傅立叶变换红外光谱来测量微塑料的物理和化学特性。结果表明,苏拉特市土壤中的多孔塑料无处不在,平均丰度为 103.2 ± 57.28 粒子 100 g-1。超过 90% 的 MP 由聚乙烯(70.19%)、聚丙烯(17.53%)和聚对苯二甲酸乙二酯(6.92%)聚合物制成。其中主要是白色/透明色的 MP,尺寸约为 2-5 毫米。主成分分析(PCA)结果表明,土壤样本中已发现的多孔塑料主要以碎片和纤维为代表,占数据集总方差的 52.90%。此外,本研究还对土壤中 MPs 的生态风险评估、MPs 的污染负荷指数(PLI)、聚合物危害指数(PHI)和生态风险指数(ERI)进行了研究。观察到 PLI 值大于 1,表明所有采样点都受到了 MPs 的污染。根据土壤样本中发现的多溴联苯醚的丰度、聚合物类型和毒性,评估得出结论,城市土壤中的多溴联苯醚污染具有中度到中度的生态风险。本研究中最大的 PHI 值为 239.69(风险类别:中),最小的 PHI 值为 8.69(风险类别:低)。使用基于软件的绿色度量分析工具(AGREE)对该方法的绿色程度进行了评估。AGREE 指标得分为 0.53,表明所开发的方法部分绿色。本研究强调了在苏拉特等大都市地区采取行动减轻 MP 污染和改进废物管理方法的极端重要性,以便在未来建立一个更绿色、更清洁的生态系统。这些发现将激励人们创造生态友好型替代品,这对减少塑料消耗、保护生态系统和减轻 MP 对土壤质量的影响至关重要。
{"title":"Extraction and Analysis of Microplastics in the Soil of Diamond City, Surat (Gujarat, India): Ecological Risk, Pollution Indices, and Greenness Evaluation","authors":"Pinal S. Bhavsar,&nbsp;Bhautik V. Chovatiya,&nbsp;Sumit B. Kamble and Anil H. Gore*,&nbsp;","doi":"10.1021/acsagscitech.4c00140","DOIUrl":"10.1021/acsagscitech.4c00140","url":null,"abstract":"<p >This study examines microplastics (MPs) for the first time in the soil of Diamond City, Surat (Gujarat, India) in terms of their abundance, distribution, chemical composition, and ecological risk assessment. A stereomicroscope and Fourier transform infrared spectroscopy were used to measure the physical and chemical properties of the MPs. The outcome showed the omnipresence of MPs in Surat city’s soil, with an average abundance of 103.2 ± 57.28 particles 100 g<sup>–1</sup>. Over 90% of all MPs were made of polyethylene (70.19%), polypropylene (17.53%), and poly(ethylene terephthalate) (6.92%) polymers. The white/transparent-colored MPs having a size of about 2–5 mm are predominantly available. The principal component analysis (PCA) results indicate that the discovered MPs in the soil samples are mostly represented by fragments and fibers, which account for 52.90% of the total variance in the data set. Additionally, in the current research, the ecological risk assessment of MPs in soil, the pollution load index (PLI), the polymer hazard index (PHI), and the ecological risk index (ERI) of MPs were studied. The PLI values greater than 1 were observed, indicating that all sampling sites were polluted with MPs. Based on the abundance, type of polymer, and toxicity of MPs found in the soil sample, an evaluation concluded that there is a moderate to medium ecological risk associated with MPs polluting the city soil. The greatest PHI value in this study is 239.69 (risk category: medium), while the lowest PHI value is 8.69 (risk category: low). The greenness profile of the method was assessed using the software-based analytical greenness metric (AGREE) tool. The developed approach is partially green, as shown by an AGREE metric score of 0.53. The present investigations emphasize the critical importance of taking action in metropolitan areas such as Surat to mitigate MP pollution and improve waste management practices for a greener and cleaner ecosystem in the future. These findings will inspire the creation of ecofriendly alternatives crucial for reducing plastic consumption, safeguarding ecosystems, and mitigating MPs’ impact on the soil quality.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 5","pages":"614–625"},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140720911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding Pesticide Exposure Mitigation Effectiveness in Achieving Endangered Species Protection Goals 了解农药暴露缓解措施在实现濒危物种保护目标方面的效果
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-09 DOI: 10.1021/acsagscitech.3c00573
R. Scott Teed*, Michael Winchell, Dwayne R. J. Moore, Bettina Miguez, Jody Stryker and Richard A. Brain, 

To protect the environment, pesticides often require mitigation measures to reduce losses via runoff, erosion, spray drift, and other routes of transport. Although many mitigation measures are generally effective at reducing pesticide losses from treated areas, it is often unknown whether they provide the desired protection level for the environment including threatened and endangered (“listed”) species. In this paper, we use the Agricultural Policy/Environmental eXtender model (APEX) to quantify reductions in pesticide losses via runoff and erosion for common mitigation measures. These measures are currently being considered for the protection of listed species in the United States (i.e., vegetative buffers, contour cropping, planting of cover crops, and no-till practices). Analyses were conducted for two pesticides, atrazine and pendimethalin, for each of the two crop groups, corn, and vegetables/ground fruits. Separate analyses were conducted for different hydrologic regions, in which these crops are grown in the United States. Our results indicate that the effectiveness of the runoff mitigation measures, whether singly or in combination, varies with pesticide, crop, and region. We also found that there are diminishing returns as more mitigations are added to the analysis. Such factors need to be considered when developing suites of potential mandatory pesticide mitigations (i.e., pick lists) for the protection of threatened and endangered species in the United States.

为了保护环境,杀虫剂通常需要采取减缓措施,以减少通过径流、侵蚀、喷洒漂移和其他 运输途径造成的损失。尽管许多减缓措施通常都能有效减少农药从施药区域的流失,但这些措施是否能为环境(包括受威胁和濒危("上市")物种)提供理想的保护水平,却往往不得而知。在本文中,我们使用农业政策/环境 eXtender 模型(APEX)来量化常见减缓措施通过径流和侵蚀减少的农药损失。这些措施目前正在美国被考虑用于保护列入清单的物种(即植被缓冲区、等高耕作、种植覆盖作物和免耕实践)。针对玉米和蔬菜/地面水果这两类作物,分别对阿特拉津和戊唑醇这两种农药进行了分析。针对美国种植这些作物的不同水文地区分别进行了分析。我们的研究结果表明,无论是单独使用还是组合使用,径流减缓措施的效果都因农药、作物和地区的不同而不同。我们还发现,随着分析中加入更多的减缓措施,收益也会递减。在为保护美国受威胁和濒危物种而制定一套潜在的强制性农药减缓措施(即选择清单)时,需要考虑这些因素。
{"title":"Understanding Pesticide Exposure Mitigation Effectiveness in Achieving Endangered Species Protection Goals","authors":"R. Scott Teed*,&nbsp;Michael Winchell,&nbsp;Dwayne R. J. Moore,&nbsp;Bettina Miguez,&nbsp;Jody Stryker and Richard A. Brain,&nbsp;","doi":"10.1021/acsagscitech.3c00573","DOIUrl":"10.1021/acsagscitech.3c00573","url":null,"abstract":"<p >To protect the environment, pesticides often require mitigation measures to reduce losses via runoff, erosion, spray drift, and other routes of transport. Although many mitigation measures are generally effective at reducing pesticide losses from treated areas, it is often unknown whether they provide the desired protection level for the environment including threatened and endangered (“listed”) species. In this paper, we use the Agricultural Policy/Environmental eXtender model (APEX) to quantify reductions in pesticide losses via runoff and erosion for common mitigation measures. These measures are currently being considered for the protection of listed species in the United States (i.e., vegetative buffers, contour cropping, planting of cover crops, and no-till practices). Analyses were conducted for two pesticides, atrazine and pendimethalin, for each of the two crop groups, corn, and vegetables/ground fruits. Separate analyses were conducted for different hydrologic regions, in which these crops are grown in the United States. Our results indicate that the effectiveness of the runoff mitigation measures, whether singly or in combination, varies with pesticide, crop, and region. We also found that there are diminishing returns as more mitigations are added to the analysis. Such factors need to be considered when developing suites of potential mandatory pesticide mitigations (i.e., pick lists) for the protection of threatened and endangered species in the United States.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 5","pages":"554–566"},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.3c00573","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140721251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultivation of Serapias orientalis Plant Using Symbiotic Methods and Investigation of Bioactive Compounds 利用共生方法栽培东方蛇莓并研究其生物活性化合物
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-04 DOI: 10.1021/acsagscitech.3c00458
Erdi Can Aytar*,  and , Yasemin Özdener Kömpe, 

Conservation biology aims to prevent the decline in biodiversity by reducing the factors that cause it. One way to achieve this is by producing economically valuable orchids in high quantities, allowing humans to rely less on natural populations. In the study, Serapias orientalis seeds were successfully germinated using symbiotic methods and transplanted into a natural field. After 18 months, the plants began to flower, marking the first successful production of S. orientalis in a natural environment. The researchers then analyzed the fatty acid and chemical contents of the leaves and flowers using gas chromatography–mass spectrometry, identifying 28 fatty acids and 19 other substances. The most abundant fatty acids were linoleic acid (21.72%), palmitic acid (20.22%), allylinolenic acid (16.87%), oleic acid (8.67%), and stearic acid (6.13%). Major compounds identified in the methanol extract included hydroxyacetic acid hydrazide (7.38%), semicarbazide hydrochloride (5.06%), dl-glyceraldehyde (5.42%), pentacosane (39.85%), and cholesterol (13.05%). These findings offer a new method for conserving S. orientalis and highlight the potential use of these plants in food, medical, and industrial applications due to their high concentration of bioactive compounds.

保护生物学旨在通过减少导致生物多样性减少的因素来防止生物多样性的减少。实现这一目标的方法之一是大量生产具有经济价值的兰花,使人类减少对自然种群的依赖。在这项研究中,东方蛇尾兰的种子通过共生方法成功发芽,并移植到一片自然田地中。18 个月后,植物开始开花,这标志着东方蛇莓首次在自然环境中成功生产。研究人员随后使用气相色谱-质谱法分析了叶片和花朵中的脂肪酸和化学成分,确定了 28 种脂肪酸和 19 种其他物质。含量最多的脂肪酸是亚油酸(21.72%)、棕榈酸(20.22%)、烯丙亚麻酸(16.87%)、油酸(8.67%)和硬脂酸(6.13%)。甲醇提取物中鉴定出的主要化合物包括羟基乙酸酰肼(7.38%)、盐酸氨基脲(5.06%)、二甘醛(5.42%)、五氯烷(39.85%)和胆固醇(13.05%)。这些发现为保护东方茜草提供了一种新方法,并强调了这些植物因其高浓度的生物活性化合物而在食品、医疗和工业应用中的潜在用途。
{"title":"Cultivation of Serapias orientalis Plant Using Symbiotic Methods and Investigation of Bioactive Compounds","authors":"Erdi Can Aytar*,&nbsp; and ,&nbsp;Yasemin Özdener Kömpe,&nbsp;","doi":"10.1021/acsagscitech.3c00458","DOIUrl":"https://doi.org/10.1021/acsagscitech.3c00458","url":null,"abstract":"<p >Conservation biology aims to prevent the decline in biodiversity by reducing the factors that cause it. One way to achieve this is by producing economically valuable orchids in high quantities, allowing humans to rely less on natural populations. In the study, <i>Serapias orientalis</i> seeds were successfully germinated using symbiotic methods and transplanted into a natural field. After 18 months, the plants began to flower, marking the first successful production of <i>S. orientalis</i> in a natural environment. The researchers then analyzed the fatty acid and chemical contents of the leaves and flowers using gas chromatography–mass spectrometry, identifying 28 fatty acids and 19 other substances. The most abundant fatty acids were linoleic acid (21.72%), palmitic acid (20.22%), allylinolenic acid (16.87%), oleic acid (8.67%), and stearic acid (6.13%). Major compounds identified in the methanol extract included hydroxyacetic acid hydrazide (7.38%), semicarbazide hydrochloride (5.06%), <span>dl</span>-glyceraldehyde (5.42%), pentacosane (39.85%), and cholesterol (13.05%). These findings offer a new method for conserving <i>S. orientalis</i> and highlight the potential use of these plants in food, medical, and industrial applications due to their high concentration of bioactive compounds.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 4","pages":"424–431"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.3c00458","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140552159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Agronomic, Nutritional Traits, and Alkaloids of Lupinus albus, Lupinus angustifolius and Lupinus luteus Genotypes: Effect of Sowing Dates and Locations 白羽扇豆、角羽扇豆和黄羽扇豆基因型的农艺性状、营养性状和生物碱:播种日期和地点的影响
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-02 DOI: 10.1021/acsagscitech.3c00581
Inês M. Valente*, André Monteiro, Carla Sousa, Carla Miranda, Margarida R. G. Maia, Carlos Castro, Ana R. J. Cabrita, Henrique Trindade and António J. M. Fonseca, 

Lupins (Lupinus spp.) are legumes with high relevance for the sustainability of agricultural systems as they improve the soil quality, namely, through the fixation of atmospheric nitrogen, and have good adaptability to different climates and soil conditions. Besides, they possess high nutritive value, especially due to the high protein content of the seeds. Nevertheless, the plants’ productivity and metabolism can be influenced by the genotype, the edaphoclimatic conditions, and the sowing practices. In this work, the effect of edaphoclimatic conditions and sowing dates on the productivity, nutritional factors, and alkaloids of the seeds of L. albus cv. Estoril, L. angustifolius cv. Tango, and L. luteus cv. Cardiga was evaluated. High variability in the seeds and protein productions, nutritional traits, and alkaloid content related to the species was observed, along with a significant effect of the location. Lupinus albus cv. Estoril showed a good compromise between productivity and low alkaloid content, being an interesting genotype for food and feed use in the conditions of this trial.

羽扇豆(羽扇豆属)是对农业系统的可持续性具有重要意义的豆科植物,因为它们可以通过固定大气中的氮来改善土壤质量,而且对不同气候和土壤条件具有良好的适应性。此外,它们还具有很高的营养价值,特别是由于种子的蛋白质含量很高。然而,植物的生产力和新陈代谢会受到基因型、气候条件和播种方法的影响。在这项研究中,我们评估了气候条件和播种日期对 L. albus cv. Estoril、L. angustifolius cv. Tango 和 L. luteus cv. Cardiga 种子产量、营养成分和生物碱的影响。结果表明,种子和蛋白质产量、营养特征和生物碱含量与品种之间存在很大差异,而且地点也有显著影响。Lupinus albus cv. Estoril在产量和低生物碱含量之间表现出很好的折衷,在本试验条件下是一种可用于食品和饲料的有趣基因型。
{"title":"Agronomic, Nutritional Traits, and Alkaloids of Lupinus albus, Lupinus angustifolius and Lupinus luteus Genotypes: Effect of Sowing Dates and Locations","authors":"Inês M. Valente*,&nbsp;André Monteiro,&nbsp;Carla Sousa,&nbsp;Carla Miranda,&nbsp;Margarida R. G. Maia,&nbsp;Carlos Castro,&nbsp;Ana R. J. Cabrita,&nbsp;Henrique Trindade and António J. M. Fonseca,&nbsp;","doi":"10.1021/acsagscitech.3c00581","DOIUrl":"https://doi.org/10.1021/acsagscitech.3c00581","url":null,"abstract":"<p >Lupins (<i>Lupinus</i> spp.) are legumes with high relevance for the sustainability of agricultural systems as they improve the soil quality, namely, through the fixation of atmospheric nitrogen, and have good adaptability to different climates and soil conditions. Besides, they possess high nutritive value, especially due to the high protein content of the seeds. Nevertheless, the plants’ productivity and metabolism can be influenced by the genotype, the edaphoclimatic conditions, and the sowing practices. In this work, the effect of edaphoclimatic conditions and sowing dates on the productivity, nutritional factors, and alkaloids of the seeds of <i>L. albus</i> cv. Estoril, <i>L. angustifolius</i> cv. Tango, and <i>L. luteus</i> cv. Cardiga was evaluated. High variability in the seeds and protein productions, nutritional traits, and alkaloid content related to the species was observed, along with a significant effect of the location. <i>Lupinus albus</i> cv. Estoril showed a good compromise between productivity and low alkaloid content, being an interesting genotype for food and feed use in the conditions of this trial.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 4","pages":"450–462"},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.3c00581","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140552152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrating Targeted Metabolomics and Targeted Proteomics to Study the Responses of Wheat Plants to Engineered Nanomaterials 整合靶向代谢组学和靶向蛋白质组学研究小麦植物对工程纳米材料的反应
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-02 DOI: 10.1021/acsagscitech.4c00046
Weiwei Li,  and , Arturo A. Keller*, 

This manuscript presents a multiomics investigation into the metabolic and proteomic responses of wheat to molybdenum (Mo)- and copper (Cu)-based engineered nanomaterials (ENMs) exposure via root and leaf application methods. Wheat plants underwent a four-week growth period with a 16 h photoperiod (light intensity set at 150 μmol·m–2·s–1), at 22 °C and 60% humidity. Six distinct treatments were applied, including control conditions alongside exposure to Mo- and Cu-based ENMs through both root and leaf routes. The exposure dosage amounted to 6.25 mg of the respective element per plant. An additional treatment with a lower dose (0.6 mg Mo/plant) of Mo ENM exclusively through the root system was introduced upon the detection of phytotoxicity. Utilizing LC–MS/MS analysis, 82 metabolites across various classes and 24 proteins were assessed in different plant tissues (roots, stems, leaves) under diverse treatments. The investigation identified 58 responsive metabolites and 19 responsive proteins for Cu treatments, 71 responsive metabolites, and 24 responsive proteins for Mo treatments, mostly through leaf exposure for Cu and root exposure for Mo. Distinct tissue-specific preferences for metabolite accumulation were revealed, highlighting the prevalence of organic acids and fatty acids in stem or root tissues, while sugars and amino acids were abundant in leaves, mirroring their roles in energy storage and photosynthesis. Joint-pathway analysis was conducted and unveiled 23 perturbed pathways across treatments. Among these, Mo exposure via roots impacted all identified pathways, whereas exposure via leaf affected 15 pathways, underscoring the reliance on exposure route of metabolic and proteomic responses. The coordinated response observed in protein and metabolite concentrations, particularly in amino acids, highlighted a dynamic and interconnected proteomic-to-metabolic-to-proteomic relationship. Furthermore, the contrasting expression patterns observed in glutamate dehydrogenase (upregulation at 1.38 ≤ FC ≤ 1.63 with high Mo dose, and downregulation at 0.13 ≤ FC ≤ 0.54 with low Mo dose) and its consequential impact on glutamine expression (7.67 ≤ FC ≤ 39.60 with high Mo dose and 1.50 ≤ FC ≤ 1.95 with low Mo dose) following Mo root exposure highlighted dose-dependent regulatory trends influencing proteins and metabolites. These findings offer a multidimensional understanding of plant responses to ENMs exposure, guiding agricultural practices and environmental safety protocols while advancing knowledge on nanomaterial impacts on plant biology.

本手稿介绍了一项多组学研究,通过根部和叶片施用方法研究了小麦对基于钼(Mo)和铜(Cu)的工程纳米材料(ENMs)暴露的代谢和蛋白质组响应。小麦植株生长期为四周,光周期为 16 小时(光照强度设定为 150 μmol-m-2-s-1),温度为 22 °C,湿度为 60%。共采用了六种不同的处理方法,包括对照条件以及通过根部和叶片接触钼和铜基 ENMs。每株植物的接触剂量为 6.25 毫克相应元素。在检测到植物毒性后,又增加了一种完全通过根系接触较低剂量(0.6 毫克钼/株)钼 ENM 的处理方法。利用 LC-MS/MS 分析,对不同处理下不同植物组织(根、茎、叶)中的 82 种不同类别的代谢物和 24 种蛋白质进行了评估。调查发现,在铜处理中,有 58 个响应代谢物和 19 个响应蛋白质;在钼处理中,有 71 个响应代谢物和 24 个响应蛋白质。研究揭示了代谢物积累的不同组织特异性偏好,突出表明有机酸和脂肪酸在茎或根组织中普遍存在,而糖和氨基酸则在叶片中大量存在,这反映了它们在能量储存和光合作用中的作用。联合途径分析揭示了 23 种不同处理的干扰途径。其中,通过根部接触钼影响了所有已确定的途径,而通过叶片接触钼则影响了 15 条途径,这突出表明了代谢和蛋白质组反应对接触途径的依赖性。在蛋白质和代谢物浓度(尤其是氨基酸浓度)中观察到的协调反应突显了蛋白质组到代谢组再到蛋白质组之间的动态和相互关联的关系。此外,在谷氨酸脱氢酶中观察到的不同表达模式(高钼剂量时上调 1.38 ≤ FC ≤ 1.63,低钼剂量时下调 0.13 ≤ FC ≤ 0.54)及其对谷氨酰胺表达的影响(高钼剂量时 7.67 ≤ FC ≤ 39.60,低钼剂量时 1.50 ≤ FC ≤ 1.95)突出了影响蛋白质和代谢物的剂量依赖性调控趋势。这些发现提供了植物对暴露于 ENMs 的反应的多维理解,可指导农业实践和环境安全协议,同时推进有关纳米材料对植物生物学影响的知识。
{"title":"Integrating Targeted Metabolomics and Targeted Proteomics to Study the Responses of Wheat Plants to Engineered Nanomaterials","authors":"Weiwei Li,&nbsp; and ,&nbsp;Arturo A. Keller*,&nbsp;","doi":"10.1021/acsagscitech.4c00046","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00046","url":null,"abstract":"<p >This manuscript presents a multiomics investigation into the metabolic and proteomic responses of wheat to molybdenum (Mo)- and copper (Cu)-based engineered nanomaterials (ENMs) exposure via root and leaf application methods. Wheat plants underwent a four-week growth period with a 16 h photoperiod (light intensity set at 150 μmol·m<sup>–2</sup>·s<sup>–1</sup>), at 22 °C and 60% humidity. Six distinct treatments were applied, including control conditions alongside exposure to Mo- and Cu-based ENMs through both root and leaf routes. The exposure dosage amounted to 6.25 mg of the respective element per plant. An additional treatment with a lower dose (0.6 mg Mo/plant) of Mo ENM exclusively through the root system was introduced upon the detection of phytotoxicity. Utilizing LC–MS/MS analysis, 82 metabolites across various classes and 24 proteins were assessed in different plant tissues (roots, stems, leaves) under diverse treatments. The investigation identified 58 responsive metabolites and 19 responsive proteins for Cu treatments, 71 responsive metabolites, and 24 responsive proteins for Mo treatments, mostly through leaf exposure for Cu and root exposure for Mo. Distinct tissue-specific preferences for metabolite accumulation were revealed, highlighting the prevalence of organic acids and fatty acids in stem or root tissues, while sugars and amino acids were abundant in leaves, mirroring their roles in energy storage and photosynthesis. Joint-pathway analysis was conducted and unveiled 23 perturbed pathways across treatments. Among these, Mo exposure via roots impacted all identified pathways, whereas exposure via leaf affected 15 pathways, underscoring the reliance on exposure route of metabolic and proteomic responses. The coordinated response observed in protein and metabolite concentrations, particularly in amino acids, highlighted a dynamic and interconnected proteomic-to-metabolic-to-proteomic relationship. Furthermore, the contrasting expression patterns observed in glutamate dehydrogenase (upregulation at 1.38 ≤ FC ≤ 1.63 with high Mo dose, and downregulation at 0.13 ≤ FC ≤ 0.54 with low Mo dose) and its consequential impact on glutamine expression (7.67 ≤ FC ≤ 39.60 with high Mo dose and 1.50 ≤ FC ≤ 1.95 with low Mo dose) following Mo root exposure highlighted dose-dependent regulatory trends influencing proteins and metabolites. These findings offer a multidimensional understanding of plant responses to ENMs exposure, guiding agricultural practices and environmental safety protocols while advancing knowledge on nanomaterial impacts on plant biology.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 4","pages":"507–520"},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140552153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An In-House X-ray Fluorescence Spectrometer Development for In Vivo Analysis of Plants 开发用于植物体内分析的内部 X 射线荧光光谱仪
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-04-01 DOI: 10.1021/acsagscitech.4c00003
Eduardo Santos, Jonatha Demetrio Gozetto, Eduardo de Almeida, Marcos Augusto Stolf Brasil, Nicolas Gustavo da Cruz da Silva, Vinicius Pires Rezende, Higor José Freitas Alves da Silva, Julia Rosatto Brandão, Gabriel Sgarbiero Montanha, José Lavres and Hudson Wallace Pereira de Carvalho*, 

X-ray fluorescence spectroscopy (XRF) is an analytical technique employed to determine the elemental composition of diverse materials. Due to its nondestructive nature and direct analysis that requires little or no sample preparation, it has been particularly useful for investigating the mineral composition of plants and soil. However, commercially available XRF benchtop equipment often restricts this type of experiment in plant science due to the volume of the sample chamber and the source–detector geometry. To overcome this problem, we developed an XRF setup that prioritizes in vivo-based experiments. The equipment is equipped with a 4 W Ag X-ray tube and a silicon drift detector. The detection limits are comparable to those of commercial instruments and suitable for evaluating plant tissues. Finally, a case study using tomato plants as a model species and rubidium (Rb+) and strontium (Sr2+) as tracers for potassium (K+) and calcium (Ca2+), respectively, demonstrated their feasibility for long-term in vivo analysis. Therefore, the present XRF system stands out as a viable and cost-effective tool for assessing the absorption and transport of minerals in plant tissues probed by time-resolved in vivo X-ray spectroscopy.

X 射线荧光光谱法(XRF)是一种用于测定各种材料元素组成的分析技术。由于 X 射线荧光光谱具有无损性质,而且直接分析几乎不需要样品制备,因此在研究植物和土壤的矿物成分方面特别有用。然而,由于样品室的体积和源-探测器的几何形状,市面上的 XRF 台式设备往往限制了植物科学中的这类实验。为了克服这一问题,我们开发了一种 XRF 设备,优先考虑基于活体的实验。该设备配备了一个 4 W 银 X 射线管和一个硅漂移探测器。其检测限与商用仪器相当,适合评估植物组织。最后,以番茄植物为模型物种,用铷(Rb+)和锶(Sr2+)分别作为钾(K+)和钙(Ca2+)的示踪剂进行的案例研究证明了它们在长期体内分析中的可行性。因此,本 XRF 系统是通过时间分辨活体 X 射线光谱法评估植物组织中矿物质的吸收和迁移的一种可行且具有成本效益的工具。
{"title":"An In-House X-ray Fluorescence Spectrometer Development for In Vivo Analysis of Plants","authors":"Eduardo Santos,&nbsp;Jonatha Demetrio Gozetto,&nbsp;Eduardo de Almeida,&nbsp;Marcos Augusto Stolf Brasil,&nbsp;Nicolas Gustavo da Cruz da Silva,&nbsp;Vinicius Pires Rezende,&nbsp;Higor José Freitas Alves da Silva,&nbsp;Julia Rosatto Brandão,&nbsp;Gabriel Sgarbiero Montanha,&nbsp;José Lavres and Hudson Wallace Pereira de Carvalho*,&nbsp;","doi":"10.1021/acsagscitech.4c00003","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00003","url":null,"abstract":"<p >X-ray fluorescence spectroscopy (XRF) is an analytical technique employed to determine the elemental composition of diverse materials. Due to its nondestructive nature and direct analysis that requires little or no sample preparation, it has been particularly useful for investigating the mineral composition of plants and soil. However, commercially available XRF benchtop equipment often restricts this type of experiment in plant science due to the volume of the sample chamber and the source–detector geometry. To overcome this problem, we developed an XRF setup that prioritizes <i>in vivo</i>-based experiments. The equipment is equipped with a 4 W Ag X-ray tube and a silicon drift detector. The detection limits are comparable to those of commercial instruments and suitable for evaluating plant tissues. Finally, a case study using tomato plants as a model species and rubidium (Rb<sup>+</sup>) and strontium (Sr<sup>2+</sup>) as tracers for potassium (K<sup>+</sup>) and calcium (Ca<sup>2+</sup>), respectively, demonstrated their feasibility for long-term <i>in vivo</i> analysis. Therefore, the present XRF system stands out as a viable and cost-effective tool for assessing the absorption and transport of minerals in plant tissues probed by time-resolved <i>in vivo</i> X-ray spectroscopy.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 4","pages":"471–477"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140552146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of DNA Reference Materials of Citrus Huanglongbing Candidatus Liberibacter asiaticus 开发柑橘黄龙病菌的 DNA 参考材料
Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-03-29 DOI: 10.1021/acsagscitech.4c00001
Xiaoyun Chen, Kai Li, Yi Ji, Ziyue Zhang, Xin Qi, Lianming Lu, Xiaofu Wang, Cheng Peng, Min Wang, Junfeng Xu* and Liang Li*, 

Citrus Huanglongbing (HLB) is a devastating disease within the Citrus industry. Candidatus Liberibacter asiaticus (CLas) is one of the most prevalent HLB-associated strains that has not been cultured in vitro. To ensure the accuracy and comparability of the molecular diagnostic method for HLB detection, certified reference materials urgently need to be developed for CLas detection. Here, we developed a series of DNA reference materials of CLas using 16S rDNA as the target gene and the SAND gene as the Citrus reference gene. The 16S rDNA gene fragment cloned by the NCBI sequence and Citrus DNA extracted by healthy Citrus leaves are thoroughly mixed for preparation. Droplet digital PCR (ddPCR) was used as an accurate quantification method for 16S rDNA, and the SAND was established and optimized through this study. Nine laboratories collaborated in determining these two parameters, and the homogeneity and stability were adequate. The quantification results demonstrated that the copy number certified values and expanded uncertainty of 16S rDNA and SAND in the high-concentration reference material were (3.86 ± 0.34) × 103 and (4.43 ± 0.39) × 103 cp/μL, respectively. The copy number certified values and expanded uncertainty of 16S rDNA and SAND in the low-concentration reference material were (3.98 ± 0.36) × 102 and (4.34 ± 0.37) × 103 cp/μL, respectively. In addition, this certified reference material will provide reliable quality control for detecting CLas.

柑橘黄龙病(HLB)是柑橘产业中的一种毁灭性病害。亚洲自由杆菌(CLas)是最普遍的 HLB 相关菌株之一,但尚未在体外培养。为确保 HLB 检测分子诊断方法的准确性和可比性,迫切需要开发出检测 CLas 的认证参考材料。在此,我们以 16S rDNA 为目标基因,以 SAND 基因为柑橘参考基因,开发了一系列 CLas DNA 参考材料。将通过 NCBI 序列克隆的 16S rDNA 基因片段与从柑橘健康叶片中提取的柑橘 DNA 充分混合后进行制备。本研究采用液滴数字 PCR(ddPCR)作为 16S rDNA 的精确定量方法,并建立和优化了 SAND 基因。九个实验室合作确定了这两个参数,其均一性和稳定性都很好。定量结果表明,高浓度参考物质中 16S rDNA 和 SAND 的拷贝数认证值和扩展不确定度分别为 (3.86 ± 0.34) × 103 cp/μL 和 (4.43 ± 0.39) × 103 cp/μL。低浓度参考物质中 16S rDNA 和 SAND 的拷贝数认证值和扩展不确定度分别为 (3.98 ± 0.36) × 102 和 (4.34 ± 0.37) × 103 cp/μL。此外,这种经认证的标准物质将为检测 CLas 提供可靠的质量控制。
{"title":"Development of DNA Reference Materials of Citrus Huanglongbing Candidatus Liberibacter asiaticus","authors":"Xiaoyun Chen,&nbsp;Kai Li,&nbsp;Yi Ji,&nbsp;Ziyue Zhang,&nbsp;Xin Qi,&nbsp;Lianming Lu,&nbsp;Xiaofu Wang,&nbsp;Cheng Peng,&nbsp;Min Wang,&nbsp;Junfeng Xu* and Liang Li*,&nbsp;","doi":"10.1021/acsagscitech.4c00001","DOIUrl":"10.1021/acsagscitech.4c00001","url":null,"abstract":"<p ><i>Citrus</i> Huanglongbing (HLB) is a devastating disease within the <i>Citrus</i> industry. <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las) is one of the most prevalent HLB-associated strains that has not been cultured in vitro. To ensure the accuracy and comparability of the molecular diagnostic method for HLB detection, certified reference materials urgently need to be developed for <i>C</i>Las detection. Here, we developed a series of DNA reference materials of <i>C</i>Las using 16S rDNA as the target gene and the SAND gene as the <i>Citrus</i> reference gene. The 16S rDNA gene fragment cloned by the NCBI sequence and <i>Citrus</i> DNA extracted by healthy <i>Citrus</i> leaves are thoroughly mixed for preparation. Droplet digital PCR (ddPCR) was used as an accurate quantification method for 16S rDNA, and the SAND was established and optimized through this study. Nine laboratories collaborated in determining these two parameters, and the homogeneity and stability were adequate. The quantification results demonstrated that the copy number certified values and expanded uncertainty of 16S rDNA and SAND in the high-concentration reference material were (3.86 ± 0.34) × 10<sup>3</sup> and (4.43 ± 0.39) × 10<sup>3</sup> cp/μL, respectively. The copy number certified values and expanded uncertainty of 16S rDNA and SAND in the low-concentration reference material were (3.98 ± 0.36) × 10<sup>2</sup> and (4.34 ± 0.37) × 10<sup>3</sup> cp/μL, respectively. In addition, this certified reference material will provide reliable quality control for detecting <i>C</i>Las.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 4","pages":"500–506"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140368699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
ACS agricultural science & technology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1