Gizem Dilara Ozdemir, Mehmet Akif Ozdemir, Mustafa Sen, Utku Kürşat Ercan
In this transformative study, machine learning (ML) and t-distributed stochastic neighbor embedding (t-SNE) are employed to interpret intricate patterns in colorimetric images of cold atmospheric plasma (CAP)-treated water. The focus is on CAP's therapeutic potential, particularly its ability to generate reactive oxygen and nitrogen species (RONS) that play a crucial role in antimicrobial activity. RGB, HSV, LAB, YCrCb, and grayscale color spaces are extracted from the colorimetric expression of oxidative stress induced by RONS, and these features are used for unsupervised ML, employing density-based spatial clustering of applications with noise (DBSCAN). The DBSCAN model's performance is evaluated using homogeneity, completeness, and adjusted rand index with a predictive data distribution graph. The best results are achieved with 3,3′,5,5′-tetramethylbenzidine–potassium iodide colorimetric assay solution immediately after plasma treatment, with values of 0.894, 0.996, and 0.826. t-SNE is further conducted for the best-case scenario to evaluate the clustering efficacy and find the best combination of features to better present the results. Correspondingly, t-SNE enhances clustering efficacy and adeptly handles challenging points. The approach pioneers dynamic and comprehensive solutions, showcasing ML's precision and t-SNE's transformative visualization. Through this innovative fusion, complex relationships are unraveled, marking a paradigm shift in biomedical analytical methodologies.
在这项变革性研究中,采用了机器学习(ML)和 t 分布随机邻域嵌入(t-SNE)来解释冷大气等离子体(CAP)处理过的水的比色图像中的复杂模式。研究重点是 CAP 的治疗潜力,尤其是其生成活性氧和氮物种 (RONS) 的能力,这种能力在抗菌活性中发挥着至关重要的作用。从 RONS 诱导的氧化应激的色度表达中提取了 RGB、HSV、LAB、YCrCb 和灰度色彩空间,并将这些特征用于无监督 ML,采用基于密度的带噪声应用空间聚类(DBSCAN)。DBSCAN 模型的性能使用同质性、完整性和调整后的兰德指数以及预测数据分布图进行评估。在血浆处理后立即使用 3,3′,5,5′-四甲基联苯胺-碘化钾比色测定溶液时,结果最佳,其值分别为 0.894、0.996 和 0.826。相应地,t-SNE 增强了聚类效果,并能巧妙地处理挑战点。这种方法开创了动态综合解决方案,展示了 ML 的精确性和 t-SNE 的变革性可视化。通过这种创新的融合,复杂的关系得以解开,标志着生物医学分析方法的范式转变。
{"title":"Unveiling the Potential: Can Machine Learning Cluster Colorimetric Images of Cold Atmospheric Plasma Treatment?","authors":"Gizem Dilara Ozdemir, Mehmet Akif Ozdemir, Mustafa Sen, Utku Kürşat Ercan","doi":"10.1002/aisy.202400029","DOIUrl":"https://doi.org/10.1002/aisy.202400029","url":null,"abstract":"<p>In this transformative study, machine learning (ML) and t-distributed stochastic neighbor embedding (t-SNE) are employed to interpret intricate patterns in colorimetric images of cold atmospheric plasma (CAP)-treated water. The focus is on CAP's therapeutic potential, particularly its ability to generate reactive oxygen and nitrogen species (RONS) that play a crucial role in antimicrobial activity. RGB, HSV, LAB, YCrCb, and grayscale color spaces are extracted from the colorimetric expression of oxidative stress induced by RONS, and these features are used for unsupervised ML, employing density-based spatial clustering of applications with noise (DBSCAN). The DBSCAN model's performance is evaluated using homogeneity, completeness, and adjusted rand index with a predictive data distribution graph. The best results are achieved with 3,3′,5,5′-tetramethylbenzidine–potassium iodide colorimetric assay solution immediately after plasma treatment, with values of 0.894, 0.996, and 0.826. t-SNE is further conducted for the best-case scenario to evaluate the clustering efficacy and find the best combination of features to better present the results. Correspondingly, t-SNE enhances clustering efficacy and adeptly handles challenging points. The approach pioneers dynamic and comprehensive solutions, showcasing ML's precision and t-SNE's transformative visualization. Through this innovative fusion, complex relationships are unraveled, marking a paradigm shift in biomedical analytical methodologies.</p>","PeriodicalId":93858,"journal":{"name":"Advanced intelligent systems (Weinheim an der Bergstrasse, Germany)","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aisy.202400029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carlo Massaroni, Loy Vitali, Daniela Lo Presti, Sergio Silvestri, Emiliano Schena
Additive manufacturing technologies increasingly revolutionize current production techniques for object manufacturing. Particularly, fused deposition modeling (FDM) strongly impacts production processes by enabling the cost-effective and efficient creation of structures with complex designs and innovative geometries. The use of conductive filaments in FDM printing is paving the way for the advancement of entirely printed sensors and circuits, although this domain is still in its early stages. In this article, the design and production of bilayer deformable pressure sensors fabricated using conductive thermoplastic polyurethane are investigated. The potential to vary the mechanical and electrical characteristics of FDM-printed components by adjusting printing parameters is explored. The influence of different levels of material infill (20%, 50%, and 100%) and different contact geometries between layers (domes, pyramids, and cylinders) is studied. Electromechanical tests are carried out to characterize the sensor, applying pressures up to 22 kPa. The 3D-printed pressure sensors demonstrate tunable mechanical and electrical sensitivities at different infill values, with the highest value of −6.3 kPa−1 achieved by using a pyramid layer at 100% infill. Sensor outputs registered during cyclic tests show reproducible responses with a wide range of sensitivity, paving the way for applicability in recording both static and periodic pressure changes.
{"title":"Fully Additively 3D Manufactured Conductive Deformable Sensors for Pressure Sensing","authors":"Carlo Massaroni, Loy Vitali, Daniela Lo Presti, Sergio Silvestri, Emiliano Schena","doi":"10.1002/aisy.202300901","DOIUrl":"https://doi.org/10.1002/aisy.202300901","url":null,"abstract":"<p>Additive manufacturing technologies increasingly revolutionize current production techniques for object manufacturing. Particularly, fused deposition modeling (FDM) strongly impacts production processes by enabling the cost-effective and efficient creation of structures with complex designs and innovative geometries. The use of conductive filaments in FDM printing is paving the way for the advancement of entirely printed sensors and circuits, although this domain is still in its early stages. In this article, the design and production of bilayer deformable pressure sensors fabricated using conductive thermoplastic polyurethane are investigated. The potential to vary the mechanical and electrical characteristics of FDM-printed components by adjusting printing parameters is explored. The influence of different levels of material infill (20%, 50%, and 100%) and different contact geometries between layers (domes, pyramids, and cylinders) is studied. Electromechanical tests are carried out to characterize the sensor, applying pressures up to 22 kPa. The 3D-printed pressure sensors demonstrate tunable mechanical and electrical sensitivities at different infill values, with the highest value of −6.3 kPa<sup>−1</sup> achieved by using a pyramid layer at 100% infill. Sensor outputs registered during cyclic tests show reproducible responses with a wide range of sensitivity, paving the way for applicability in recording both static and periodic pressure changes.</p>","PeriodicalId":93858,"journal":{"name":"Advanced intelligent systems (Weinheim an der Bergstrasse, Germany)","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aisy.202300901","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dynamic and agile locomotion in legged robots enables them to overcome obstacles and navigate complex and unstructured terrain. However, the leg mechanisms and actuators needed for versatile locomotion are much more challenging to manufacture and integrate in sub-gram scale robots. Herein, Picotaur, a 15.4 mg hexapedal robot with legs that enable various locomotion tasks such as turning, climbing 3D-printed stairs, and pushing loads for the first time at these size scales, is presented. 3D printing with two-photon polymerization enables the manufacture of electrostatically driven 2 degrees of freedom legs on a robot body made from a flexible printed circuit board. Based on simple control inputs, Picotaur can achieve alternating tripod gaits, reaching speeds up to 57 mm (7.2 body lengths) per second, as well as pronking gaits to tackle a wider variety of terrain. This approach to manufacturing and controlling legged robots at smaller scales provides a path forward toward robots that can be used for practical applications ranging from inspection to exploration and rival the performance of insects at similar size scales.
{"title":"Picotaur: A 15 mg Hexapedal Robot with Electrostatically Driven, 3D-Printed Legs","authors":"Sukjun Kim, Aaron M. Johnson, Sarah Bergbreiter","doi":"10.1002/aisy.202400196","DOIUrl":"https://doi.org/10.1002/aisy.202400196","url":null,"abstract":"<p>Dynamic and agile locomotion in legged robots enables them to overcome obstacles and navigate complex and unstructured terrain. However, the leg mechanisms and actuators needed for versatile locomotion are much more challenging to manufacture and integrate in sub-gram scale robots. Herein, Picotaur, a 15.4 mg hexapedal robot with legs that enable various locomotion tasks such as turning, climbing 3D-printed stairs, and pushing loads for the first time at these size scales, is presented. 3D printing with two-photon polymerization enables the manufacture of electrostatically driven 2 degrees of freedom legs on a robot body made from a flexible printed circuit board. Based on simple control inputs, Picotaur can achieve alternating tripod gaits, reaching speeds up to 57 mm (7.2 body lengths) per second, as well as pronking gaits to tackle a wider variety of terrain. This approach to manufacturing and controlling legged robots at smaller scales provides a path forward toward robots that can be used for practical applications ranging from inspection to exploration and rival the performance of insects at similar size scales.</p>","PeriodicalId":93858,"journal":{"name":"Advanced intelligent systems (Weinheim an der Bergstrasse, Germany)","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aisy.202400196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142443604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}