Biological research for nursing最新文献

Biomarkers may complement patient-reported outcomes by providing objective insight into the multidimensional nature of chronic pain. The urine metabolite pain indicator (UMPI), a composite of four urine metabolites, has previously demonstrated cross-sectional associations with pain-related symptoms; however, it is unclear whether these associations are consistent over time. This study evaluated whether the UMPI and its individual metabolites remain associated with chronic pain outcomes following intervention, which would support its potential as a monitoring biomarker. A secondary analysis of a pragmatic clinical trial, where 148 active-duty service members with chronic pain provided urine samples and completed 11 self-reported outcome measures at baseline and 6-week follow-up after either a complementary and integrative health or a standard rehabilitative care pain intervention. Associations were examined using linear regression and mixed models. The UMPI remained significantly associated with physical function and overall pain impact post-intervention. Ethylmalonic acid showed associations with fatigue, anger, and physical function. Notably, methylmalonate, while not significant at baseline, had the most robust association with pain post-intervention, including significant correlations with both physical function and pain impact. Kynurenic acid was associated with sleep-related impairment. Pyroglutamic acid showed no significant adjusted associations. No statistically significant differences in metabolite and symptom correlations were found between time points, suggesting that these associations remained consistent over the short term. The UMPI may serve as a reliable, noninvasive index biomarker for tracking chronic pain burden, particularly physical outcomes. Findings support integrating the UMPI into biologically informed chronic pain assessment.

Background: Inflammation is essential for skeletal muscle repair following acute injury, and successful muscle regeneration requires a balance between pro- and anti-inflammatory signaling. Estrogen regulates inflammatory responses, suggesting a role in tissue repair. CD169+ macrophages are also associated with tissue repair. However, these cells and related interferon-inducible genes have not been investigated in regenerating muscle. Objective: To examine how 17β-estradiol treatment influences the expression and co-expression network of Siglec1 (encoding CD169), an interferon-inducible gene, during muscle regeneration. Methods: Ovariectomized mice received either 17β-estradiol or placebo before undergoing a standardized crush injury to hindlimb muscle groups and exposure to simulated flight. Mice were euthanized at 32-h, 96-h, or 192-h postinjury. Differential gene expression analysis was performed on injured muscles to assess treatment-related transcriptional changes, and additional muscles were evaluated for regeneration stage and CD169 protein expression. Results: At 192-h postinjury, Siglec1 was among the top upregulated genes in 17β-estradiol-treated mice relative to placebo mice, which reflected a decline in Siglec1 expression over time in the placebo group. Correlation analysis revealed that Siglec1 was strongly associated with interferon-related genes under placebo treatment, whereas the connectivity of Siglec1 under 17β-estradiol treatment was weakened. Immunohistochemistry confirmed stronger CD169 staining in regenerating muscle of 17β-estradiol-treated mice. Conclusion: Siglec1 expression is more stable in an estrogen state compared to an ovarian-hormone-deficient state. Understanding how estrogen deficiency alters inflammatory signaling after muscle injury may inform interventions to promote recovery in postmenopausal women, who may be at risk for impaired muscle repair.

Background: For immigrant mothers navigating the challenges of an unfamiliar sociopolitical landscape, the burden of discrimination extends beyond their personal experiences. Over time, cumulative exposure to discrimination may shape the environments in which young children grow and develop, with potential biological effects across the lifespan. Objectives: This cross-sectional observational study examined (1) whether maternal experiences of post-migration discrimination in the past year are associated with pro-inflammatory gene expression in their preschool-aged children, and (2) the potential contributing roles of maternal depressive symptoms and child body mass index (BMI). Methods: Immigrant Latina mothers completed sociodemographic and psychosocial measures (acculturation, discrimination, depressive symptoms). Buccal swabs were collected from children to assess mRNA expression of pro-inflammatory genes (IL-8, IL-1β), and child BMI was measured. Analyses included 70 mother-child dyads with ≥1 valid biomarker. We fit a multivariate model with correlated residuals across biomarkers to test associations between experiences of maternal discrimination and child gene expression. Results: Most mothers (69%) reported experiencing discrimination in the past year, primarily due to race or ancestry. Children whose mothers reported more frequent experiences of discrimination had higher IL-8 (B = .05, SE = .01, p < .001) and IL-1β (Β = .03, SE = .01, p = .03) mRNA expression. Associations persisted after adjusting for maternal depressive symptoms and child BMI. Conclusions: Early life experiences that devalue immigrants and their children may be critical predictors of health disparities that develop after migration. Cultivating welcoming environments for immigrant families may help support children's potential and broader community resilience.

ObjectiveTo analyze various innovative approaches in the supportive therapy of patients with thalassemia and to summarize the efficacy of different biomedical interventions from Randomized Controlled Trials (RCT).MethodsThis systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) reporting guidelines and registered in PROSPERO (CRD420251163054). A comprehensive literature search was conducted across four major databases: Scopus, PubMed, ScienceDirect, and Sage Journals. The inclusion criteria for this review involve patients with thalassemia, focus on the supportive therapy of thalassemia, conducted in healthcare settings, and employ a RCT design. The literature quality was evaluated using the Joanna Briggs Institute (JBI) tool for RCT. The data were synthesized using a narrative synthesis approach.ResultsFrom 2,394 records, 13 articles were identified. Luspatercept and Thalidomide were found to be the most effective in reducing transfusion dependence and increasing hemoglobin levels. For iron overload, Amlodipine and Spirulina significantly improved cardiac T2*, contributing to cardioprotection. Furthermore, Curcumin supplementation was shown to improve lipid metabolism and inhibit systemic inflammation. Meanwhile, therapies using Green Tea and Vitamin E were effective in reducing markers of cellular oxidative damage, with Green Tea also demonstrating dual efficacy in reducing serum ferritin (SF) and liver iron concentration (LIC).ConclusionOverall, new and adjuvant supportive therapies significantly support the biological management of patients with thalassemia. Further studies are required to integration between pharmacological therapies and nutraceutical approaches should be further explored to optimize improvements in hematological status, oxidative stress, and patients' metabolic profiles.

Background: Adolescent hypertension is an emerging global issue, with metabolic and inflammatory dysregulation potentially preceding cardiovascular disease. Increasing soft drinks and fast food consumption has been associated with obesity and metabolic imbalance. However, the biological pathways connecting these dietary behaviours to blood pressure remain weakly understood in Indonesian adolescents. Objectives: To investigate mediating effects of body mass index, uric acid, and neutrophil-to-lymphocyte ratio on relationship between soft-drink and fast-food consumption and systolic blood pressure among Indonesian adolescents and young adults. Methods: A nationwide, community-based cross-sectional study was conducted among 500 adolescents aged 10-24 years, representing Indonesia's major ethnic regions. Dietary intake was assessed using semi-quantitative food frequency questionnaire. Clinical measurements included body mass index, uric acid, neutrophil-to-lymphocyte ratio, and systolic blood pressure. Bivariate and multivariate analyses followed by mediation and multi-group analyses. Results: The mean systolic blood pressure was 110.84mmHg; soft-drink consumption correlated with uric acid, Body Mass Index, and systolic blood pressure (p < 0.001). In multiple regression, Body Mass Index (β = 0.36, p < 0.01) and soft drink-fast food consumption interaction (β = 0.27, p < 0.01) independently predicted systolic blood pressure. A significant indirect effect of soft-drink consumption on systolic blood pressure mediated by Body Mass Index, Neutrophil-to- Lymphocyte Ratio, and uric acid (β = 0.02, p < 0.05). Multi-group analysis indicated that effects of fast-food and soft-drink intake on body mass index and neutrophil-to-lymphocyte ratio did not differ significantly across gender or uric acid. Conclusions: Soft-drink consumption indirectly increases systolic blood pressure through metabolic and inflammatory mechanisms. Early nursing-led interventions that promote healthy diets, metabolic screening, and school-based health education are crucial to mitigating subclinical cardiovascular risk.

The higher risk for cancer documented among night-shift nurses compared to the day-shift nurses possibly results from lower melatonin levels associated with exposure to light at night in the work environment. Research demonstrates that eliminating spectral power <530 nm to both eyes prevents light-induced nocturnal melatonin suppression, and complete occlusion of one eye reduces melatonin suppression relative to that when both eyes are open. This study investigated whether filtering short wavelengths from one eye using a blue-blocking filter, rather than occluding one eye, would maintain high melatonin levels, alertness, and visual performance. This crossover, within-subjects study ran for six nights. Subjects experienced one of six light conditions involving combinations of binocular versus monocular and filtered versus unfiltered vision per night. Normalized melatonin concentration area under the curve (AUC_n) served as the primary outcome and the numerical verification (NVT) and go/no-go (GNG) performance tasks, pupil area, and subjective sleepiness (Karolinska Sleepiness Scale [KSS]) served as secondary outcomes. Both filtered and unfiltered monocular light conditions resulted in significantly greater melatonin AUC_n than the unfiltered binocular light condition. Performance at the NVT and at the GNG was not affected by filtering one eye compared to the unfiltered binocular light condition, nor did it affect KSS ratings. Melatonin AUC_n positively correlated with pupil area for corresponding light conditions. In conclusion, filtering one eye can help preserve melatonin levels in night workers while maintaining alertness and visual performance, which could be a simple and effective solution for improving health in night-shift nurses.

The hemoglobin-albumin-lymphocyte-platelet (HALP) index, a composite marker reflecting nutritional, inflammatory, and immune status, has been linked to various health outcomes. However, its role in predicting deep vein thrombosis (DVT) occurrence in stroke patients and its potential influence on coagulation biomarkers, particularly D-dimer, remain poorly understood. This study aims to investigate the association between the HALP index and the occurrence of DVT in stroke patients, exploring the potential moderating role of HALP in regulating D-dimer levels and DVT formation. A retrospective study was conducted with 611 stroke patients, categorized into quartiles based on HALP scores. Multivariate logistic regression, restricted cubic spline (RCS) analysis, and subgroup analyses stratified by age, gender, body mass index (BMI), and D-dimer levels were performed to assess the relationship between HALP and DVT. Moderation analysis was used to explore the regulatory effect of HALP on D-dimer levels. HALP levels were inversely associated with DVT occurrence. Patients in higher HALP quartiles (Q3: 33.40-47.93, Q4: 47.93-152.58) exhibited significantly lower odds of DVT (p < .001). RCS modeling identified a threshold effect, with a sharp increase in DVT occurrence below a HALP level of 33.5. Subgroup analyses revealed that the protective association of higher HALP scores with reduced DVT incidence was consistent across all age groups and was significant in patients with both low and high D-dimer levels. This protective association was particularly strong in males (OR = 0.148) and overweight patients (OR = 0.089). The interaction between HALP and D-dimer was significant (p < .001), with HALP moderating the association between D-dimer and DVT occurrence. HALP is a strong, independent predictor of post-stroke DVT and moderates the prothrombotic impact of D-dimer. As a low-cost, integrative marker, HALP offers clinical value for early DVT occurrence stratification and individualized prevention strategies in stroke patients.

This research intends to provide a comprehensive review of existing literature on sleep within the nursing field, employing bibliometric methods. A comprehensive analysis of the available literature concerning sleep in nursing was carried out through the Web of Science Core Collection (WoSCC) on October 20, 2025. The search terms "sleep" and "nursing" played a crucial role in identifying relevant research studies. VOSviewer was applied for bibliometric analysis and visualization to identify authors, journals, countries and display the keyword co-occurrence networks. A total of 2.456 publications focused on the topic of sleep were identified from 1991 to 2025. The dataset comprised predominantly research articles (2.296, 93.5%). These publications appeared in a total of 191 distinct academic journals and collectively received 103,025 citations over the analyzed period. The number of publications increased by an average of 6.83 units per year during the observed period (R² = 0.9622; p < 0.001). In the keyword co-occurrence network, "sleep," "sleep quality," and "nursing" keywords the highest frequency. The Journal of Clinical Nursing exhibited the highest publication output with 109 articles. The United States accounted for the largest share of publications. Development and Adaptation of Sleep Instruments in Nursing was performed on 14 measurement tools. This bibliometric study revealed the research points and trends in the field of sleep in nursing research. Future research should aim to strengthen international collaboration among nurse researchers, leveraging artificial intelligence tools and wearable technologies to effectively address sleep-related issues and enhance sleep quality.

Introduction: Psychosocial stressors are linked with declined physical and psychological health, and short telomere length (TL). While positive social interactions have been linked to improved postpartum physical and mental health, little research has explored the longitudinal effects of psychosocial stressors and telomere dynamics in the sensitive postpartum period. This study aimed to examine the effect of positive and negative social interactions on TL among first-time mothers during the first year postpartum. Methods: A cohort of 103 first-time mothers from Northern Israel completed validated measures assessing social support, marital satisfaction, and depressive symptoms, and provided blood samples for leukocyte TL analysis. Hierarchical linear and logistic regressions, adjusting for covariates, examined the association between social interactions and change in TL. Results: We observed three patterns of telomere dynamics over the year: telomere shortening, moderate telomere lengthening, and relatively high telomere lengthening in the lower, medium, and upper tertiles of change in TL, respectively, used as our categorical outcome. Hierarchical logistic regressions analyses revealed that mothers with higher marital satisfaction had a 4-fold greater likelihood of substantial telomere lengthening (OR = 3.997, 95%CI [1.345, 11.876] p = .013), remaining significant after correction for multiple comparisons. Negative social interactions showed initial associations (OR = 0.332, 95%CI [0.114, 0.971] p = .044) but did not maintain significance after multiple comparison adjustment. Discussion: Marital satisfaction showed a robust association with telomere lengthening postpartum, representing the most consistent finding. Social relationships, particularly intimate partnership quality, may be important in the postpartum period, not just for mental health but for biological aging indices.

Most infants are diagnosed with hyperbilirubinemia in the first day of life. Although phototherapy is the most common treatment used in hyperbilirubinemia, different alternative therapies such as massage are also used. This systematic review and meta-analysis was designed to evaluate the effect of massage on serum bilirubin levels, defecation frequency, and length of hospital stay in neonates with hyperbilirubinemia receiving phototherapy. A review of randomized controlled trials published in English from 2008 to November 2023 was conducted using PubMed-Medline, Academic Search Ultimate-EBSCOhost, Web of Science, and Scopus databases. From 217 identified trials, six randomized controlled studies were included, with a total sample of 394 term infants. Infant massage was effective in increasing defecation frequency (MD: 0.52, 95% CI: Min: 0.29- Max: 0.76, p < 0.0001), reducing serum bilirubin levels on the 3rd and 4th days (MD: -1.19, 95% CI: Min: -1.50- Max: -0.89, p < 0.0001) (MD:-2.33, 95% CI: Min: -2.88- Max: -1.79, p < 0.0001), and shortening hospital stay (MD:-13.66, 95% CI: Min: -16.74- Max: -10.59, p < 0.0001). This systematic review and meta-analysis suggest that infant massage is a safe and effective intervention for term neonates with hyperbilirubinemia, contributing to a shorter hospital stay by lowering serum bilirubin levels and increasing defecation frequency.