首页 > 最新文献

Journal of hazardous materials最新文献

英文 中文
Neurotoxicity and intestinal microbiota dysbiosis induced by per- and polyfluoroalkyl substances in crucian carp (Carassius auratus). 全氟烷基和多氟烷基物质对鲫鱼的神经毒性和肠道微生物菌群失调。
Pub Date : 2024-10-05 Epub Date: 2024-08-22 DOI: 10.1016/j.jhazmat.2024.135611
Yang Zhao, Minyu Wang, Weihua Chu

Per- and polyfluoroalkyl substances (PFAS) have been called "forever chemicals" due to their inherent chemical stability. Their potential toxic effects on aquatic animals and health risk assessments have not been fully elucidated. In this study, we investigated the toxic effects of PFASs at environmentally relevant concentrations (200 ng/L) on crucian carp (Carassius auratus). The results showed that PFAS reduced the comfort behaviour of crucian carp and was associated with reduced levels of acetylcholinesterase and dopamine in the brain. PFAS exposure also decreased the activities of total superoxide dismutase, catalase and glutathione peroxidase, while increasing the levels of malondialdehyde. PFAS caused over-expression of the pro-inflammatory cytokines TNF-α, IFN-γ and stress-related genes Caspase-3, HSP-70 in the fish brain. Pathological staining showed that PFAS caused multifocal demyelination and perineural vacuolization in brain, intestinal tissue also showed reduced villus length and focal damage. PFASs altered the composition of the gut microbiota of crucian carp, significantly increasing the abundance of potentially pathogenic bacteria and the potential pathogenicity of the microbiota. It is suggested that PFASs may cause varying degrees of tissue damage by destabilising the gut microbiota. These results provide insights for assessing the toxicity of PFAS contaminants at aquatic environmental concentrations.

全氟和多氟烷基物质(PFAS)因其固有的化学稳定性而被称为 "永远的化学品"。它们对水生动物的潜在毒性影响和健康风险评估尚未完全阐明。本研究调查了 PFAS 在环境相关浓度(200 纳克/升)下对鲫鱼的毒性影响。结果表明,PFAS 会降低鲫鱼的舒适行为,并与大脑中乙酰胆碱酯酶和多巴胺水平的降低有关。接触 PFAS 还会降低总超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性,同时增加丙二醛的含量。PFAS 导致鱼类大脑中促炎细胞因子 TNF-α、IFN-γ 和应激相关基因 Caspase-3、HSP-70 过度表达。病理染色显示,PFAS 导致脑部多灶性脱髓鞘和神经周围空泡化,肠道组织也出现绒毛长度缩短和灶性损伤。PFASs 改变了鲫鱼肠道微生物群的组成,显著增加了潜在致病菌的数量和微生物群的潜在致病性。研究表明,PFASs 可能会通过破坏肠道微生物群的稳定性而造成不同程度的组织损伤。这些结果为评估 PFAS 污染物在水生环境浓度下的毒性提供了启示。
{"title":"Neurotoxicity and intestinal microbiota dysbiosis induced by per- and polyfluoroalkyl substances in crucian carp (Carassius auratus).","authors":"Yang Zhao, Minyu Wang, Weihua Chu","doi":"10.1016/j.jhazmat.2024.135611","DOIUrl":"10.1016/j.jhazmat.2024.135611","url":null,"abstract":"<p><p>Per- and polyfluoroalkyl substances (PFAS) have been called \"forever chemicals\" due to their inherent chemical stability. Their potential toxic effects on aquatic animals and health risk assessments have not been fully elucidated. In this study, we investigated the toxic effects of PFASs at environmentally relevant concentrations (200 ng/L) on crucian carp (Carassius auratus). The results showed that PFAS reduced the comfort behaviour of crucian carp and was associated with reduced levels of acetylcholinesterase and dopamine in the brain. PFAS exposure also decreased the activities of total superoxide dismutase, catalase and glutathione peroxidase, while increasing the levels of malondialdehyde. PFAS caused over-expression of the pro-inflammatory cytokines TNF-α, IFN-γ and stress-related genes Caspase-3, HSP-70 in the fish brain. Pathological staining showed that PFAS caused multifocal demyelination and perineural vacuolization in brain, intestinal tissue also showed reduced villus length and focal damage. PFASs altered the composition of the gut microbiota of crucian carp, significantly increasing the abundance of potentially pathogenic bacteria and the potential pathogenicity of the microbiota. It is suggested that PFASs may cause varying degrees of tissue damage by destabilising the gut microbiota. These results provide insights for assessing the toxicity of PFAS contaminants at aquatic environmental concentrations.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135611"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142038060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effectivity and applicability of a novel sugar-based anionic and nonionic Gemini surfactant synthetized for the perchloroethylene-contaminated groundwater remediation. 合成的一种新型糖基阴离子和非离子 Gemini 表面活性剂对全氯乙烯污染地下水修复的有效性和适用性。
Pub Date : 2024-10-05 Epub Date: 2024-08-08 DOI: 10.1016/j.jhazmat.2024.135458
Yu Yao, Yufeng Fu, Chengwu Zhang, Hui Zhang, Chuanyu Qin

Surfactant-enhanced aquifer remediation (SEAR) has effectively removed dense nonaqueous phase liquids (DNAPLs) from the contaminated aquifers. However, restricted by structural defects, typical monomeric surfactants undergo precipitation, high adsorption loss, and poor solubilization in aquifers, resulting in low remediation efficiency. In this study, a novel sugar-based anionic and non-ionic Gemini surfactant (SANG) was designed and synthesized for SEAR. Glucose was introduced into SANG as a non-ionic group to overcome the interference of low temperature and ions in groundwater. Sodium sulfonate was introduced as an anionic group to overcome aquifer adsorption loss. Two long-straight carbon chains were introduced as hydrophobic groups to provide high surface activity and solubilizing capacity. Even with low temperature or high salt content, its solution did not precipitate in aquifer conditions. The adsorption loss was as low as 0.54 and 0.90 mg/g in medium and fine sand, respectively. Compared with typical surfactants used for SEAR, SANG had the highest solubilization and desorption abilities for perchloroethylene (PCE) without emulsification, a crucial negative that Tween80 and other non-ionic surfactants exhibit. After flushing the contaminated aquifer using SANG, > 99 % of PCE was removed. Thus, with low potential environmental risk, SANG is effectively applicable in subsurface remediation, making it a better surfactant choice for SEAR.

表面活性剂强化含水层修复(SEAR)可有效去除受污染含水层中的高密度非水相液体(DNAPLs)。然而,受结构缺陷的限制,典型的单体表面活性剂在含水层中会发生沉淀、吸附损失大、溶解度低,从而导致修复效率低下。本研究为 SEAR 设计并合成了一种新型糖基阴离子和非离子 Gemini 表面活性剂(SANG)。SANG 中引入了葡萄糖作为非离子基团,以克服低温和地下水中离子的干扰。引入磺酸钠作为阴离子基团,以克服含水层的吸附损失。引入两条长直碳链作为疏水基团,以提供高表面活性和增溶能力。即使在低温或高含盐量的条件下,其溶液在含水层中也不会沉淀。在中砂和细砂中的吸附损耗分别低至 0.54 和 0.90 毫克/克。与 SEAR 使用的典型表面活性剂相比,SANG 对全氯乙烯(PCE)的增溶和解吸能力最强,且不会发生乳化现象,而 Tween80 和其他非离子表面活性剂都会出现乳化现象。使用 SANG 冲洗受污染的含水层后,99% 以上的 PCE 被去除。因此,SANG 具有较低的潜在环境风险,可有效用于地下修复,是 SEAR 的最佳表面活性剂选择。
{"title":"The effectivity and applicability of a novel sugar-based anionic and nonionic Gemini surfactant synthetized for the perchloroethylene-contaminated groundwater remediation.","authors":"Yu Yao, Yufeng Fu, Chengwu Zhang, Hui Zhang, Chuanyu Qin","doi":"10.1016/j.jhazmat.2024.135458","DOIUrl":"10.1016/j.jhazmat.2024.135458","url":null,"abstract":"<p><p>Surfactant-enhanced aquifer remediation (SEAR) has effectively removed dense nonaqueous phase liquids (DNAPLs) from the contaminated aquifers. However, restricted by structural defects, typical monomeric surfactants undergo precipitation, high adsorption loss, and poor solubilization in aquifers, resulting in low remediation efficiency. In this study, a novel sugar-based anionic and non-ionic Gemini surfactant (SANG) was designed and synthesized for SEAR. Glucose was introduced into SANG as a non-ionic group to overcome the interference of low temperature and ions in groundwater. Sodium sulfonate was introduced as an anionic group to overcome aquifer adsorption loss. Two long-straight carbon chains were introduced as hydrophobic groups to provide high surface activity and solubilizing capacity. Even with low temperature or high salt content, its solution did not precipitate in aquifer conditions. The adsorption loss was as low as 0.54 and 0.90 mg/g in medium and fine sand, respectively. Compared with typical surfactants used for SEAR, SANG had the highest solubilization and desorption abilities for perchloroethylene (PCE) without emulsification, a crucial negative that Tween80 and other non-ionic surfactants exhibit. After flushing the contaminated aquifer using SANG, > 99 % of PCE was removed. Thus, with low potential environmental risk, SANG is effectively applicable in subsurface remediation, making it a better surfactant choice for SEAR.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135458"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142038064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impacts of chloride ions on the electrochemical decomplexation and degradation of Cr(III)-EDTA: Reaction mechanisms of HO and RCS. 氯离子对 Cr(III)-EDTA 电化学解络合和降解的影响:HO- 和 RCS 的反应机制。
Pub Date : 2024-10-05 Epub Date: 2024-08-25 DOI: 10.1016/j.jhazmat.2024.135636
Shujie Zheng, Jiani Yao, Ying Huang, Jiaqi Ren, Yang Hou, Bin Yang, Lecheng Lei, Jianjie Fu, Abdulaziz Al-Anazi, Guibin Jiang, Zhongjian Li

The removal of Cr(III)-organic complexes, encompassing both decomplexation and ligand degradation, presents significant challenges in industrial wastewater treatment. As one of the most common anions in wastewater, Cl- significantly improves the efficiency of electrochemically removing Cr(III)-organic complexes through generated reactive chlorine species (RCS). In the electrochemical chlorine (EC/Cl2) process, extensive experimentation revealed that ClO plays a dominant role in the degradation of Cr(III)-EDTA, surpassing the effects of free chlorine, direct electrooxidation, HO, and other RCS. Density functional theory calculations indicated that RCS, primarily Cl and ClO, preferentially oxidize the ligand in Cr(III)-EDTA via H-abstraction, whereas HO trends to attack the Cr atom through electron transfer. The influential factors on the degradation efficiency of Cr(III)-EDTA, Cr(VI) yield, and total organic carbon removal in EC/Cl2 were also assessed, including Cl- concentration, current density, and pH. Real industrial wastewater was employed as a reaction matrix to evaluate the application of the EC/Cl2 process for treating Cr(III)-EDTA, accompanied by energy efficiency calculations. Additionally, a two-chamber reactor was established to simultaneously oxidize Cr(III)-EDTA at the anode and reduce Cr(VI) at the cathode. This study provided insight into developing RCS-dominated AOPs to effectively decomplex and decompose organic Cr(III)-complexes in Cl--containing industrial wastewater.

铬(III)有机络合物的去除包括解络合和配体降解,这给工业废水处理带来了巨大挑战。作为废水中最常见的阴离子之一,Cl- 可通过生成活性氯(RCS)显著提高电化学去除铬(III)有机络合物的效率。在电化学氯(EC/Cl2)过程中,大量实验表明,ClO- 在降解 Cr(III)-EDTA 的过程中起着主导作用,超过了游离氯、直接电氧化、HO- 和其他 RCS 的作用。密度泛函理论计算表明,RCS(主要是 Cl- 和 ClO-)优先通过 H-萃取氧化 Cr(III)-EDTA 中的配体,而 HO- 则倾向于通过电子转移攻击 Cr 原子。此外,还评估了 EC/Cl2 中对 Cr(III)-EDTA 降解效率、Cr(VI)产量和总有机碳去除率的影响因素,包括 Cl- 浓度、电流密度和 pH 值。实际工业废水被用作反应矩阵,以评估 EC/Cl2 工艺在处理 Cr(III)-EDTA 方面的应用,同时进行能效计算。此外,还建立了一个双室反应器,以同时在阳极氧化 Cr(III)-EDTA 和在阴极还原 Cr(VI)。这项研究为开发以 RCS 为主导的 AOPs 提供了深入的见解,从而有效地解聚和分解含氯工业废水中的有机铬(III)络合物。
{"title":"Impacts of chloride ions on the electrochemical decomplexation and degradation of Cr(III)-EDTA: Reaction mechanisms of HO<sup>•</sup> and RCS.","authors":"Shujie Zheng, Jiani Yao, Ying Huang, Jiaqi Ren, Yang Hou, Bin Yang, Lecheng Lei, Jianjie Fu, Abdulaziz Al-Anazi, Guibin Jiang, Zhongjian Li","doi":"10.1016/j.jhazmat.2024.135636","DOIUrl":"10.1016/j.jhazmat.2024.135636","url":null,"abstract":"<p><p>The removal of Cr(III)-organic complexes, encompassing both decomplexation and ligand degradation, presents significant challenges in industrial wastewater treatment. As one of the most common anions in wastewater, Cl<sup>-</sup> significantly improves the efficiency of electrochemically removing Cr(III)-organic complexes through generated reactive chlorine species (RCS). In the electrochemical chlorine (EC/Cl<sub>2</sub>) process, extensive experimentation revealed that ClO<sup>•</sup> plays a dominant role in the degradation of Cr(III)-EDTA, surpassing the effects of free chlorine, direct electrooxidation, HO<sup>•</sup>, and other RCS. Density functional theory calculations indicated that RCS, primarily Cl<sup>•</sup> and ClO<sup>•</sup>, preferentially oxidize the ligand in Cr(III)-EDTA via H-abstraction, whereas HO<sup>•</sup> trends to attack the Cr atom through electron transfer. The influential factors on the degradation efficiency of Cr(III)-EDTA, Cr(VI) yield, and total organic carbon removal in EC/Cl<sub>2</sub> were also assessed, including Cl<sup>-</sup> concentration, current density, and pH. Real industrial wastewater was employed as a reaction matrix to evaluate the application of the EC/Cl<sub>2</sub> process for treating Cr(III)-EDTA, accompanied by energy efficiency calculations. Additionally, a two-chamber reactor was established to simultaneously oxidize Cr(III)-EDTA at the anode and reduce Cr(VI) at the cathode. This study provided insight into developing RCS-dominated AOPs to effectively decomplex and decompose organic Cr(III)-complexes in Cl<sup>-</sup>-containing industrial wastewater.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135636"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polystyrene nanoplastics alter intestinal toxicity of 2,4-DTBP in a sex-dependent manner in zebrafish (Danio rerio). 聚苯乙烯纳米塑料以性别依赖的方式改变斑马鱼(Danio rerio)肠道中 2,4-DTBP 的毒性。
Pub Date : 2024-10-05 Epub Date: 2024-08-22 DOI: 10.1016/j.jhazmat.2024.135585
Ruimin Liu, Huina Gao, Xuefang Liang, Jiye Zhang, Qingjian Meng, Yuchen Wang, Wei Guo, Christopher J Martyniuk, Jinmiao Zha

Nanoplastics (NPs) and 2,4-di-tert-butylphenol (2,4-DTBP) are ubiquitous emerging environmental contaminants detected in aquatic environment. While the intestinal toxicity of 2,4-DTBP alone has been studied, its combined effects with NPs remain unclear. Herein, adult zebrafish were exposed to 80 nm polystyrene nanoplastics (PS-NPs) or/ and 2,4-DTBP for 28 days. With co-exposure of PS-NPs, impact of 2,4-DTBP on feeding capacity and intestinal histopathology was enhanced in males while attenuated in females. Addition of PS-NPs significantly decreased the uptake of 2,4-DTBP in females, while the intestinal concentrations of 2,4-DTBP were not different between the sexes in co-exposure groups. Furthermore, lower intestinal pH and higher contents of digestive enzymes were detected in male fish, while bile acid was significantly increased in co-exposed females. In addition, co-exposure of PS-NPs stimulated female fish to remodel microbial composition to potentially enhance xenobiotics degradation, while negative Aeromonas aggravated inflammation in males. These results indicated that in the presence of PS-NPs, the gut microenvironment in females can facilitate the detoxification of 2,4-DTBP, while exaggerating toxiciy in males. Overall, this study demonstrates that toxicological outcomes of NPs-chemical mixtures may be modified by sex-specific physiology and microbiota composition, furthering understanding for environmental risk assessment and management of aquatic environments.

纳米塑料(NPs)和 2,4-二叔丁基苯酚(2,4-DTBP)是在水生环境中检测到的无处不在的新兴环境污染物。虽然 2,4-DTBP 本身的肠道毒性已被研究过,但其与 NPs 的联合效应仍不清楚。在这里,成年斑马鱼暴露于 80 纳米聚苯乙烯纳米塑料(PS-NPs)或/和 2,4-DTBP 28 天。在同时暴露 PS-NPs 的情况下,2,4-DTBP 对雄性斑马鱼摄食能力和肠道组织病理学的影响增强,而对雌性斑马鱼的影响减弱。添加 PS-NPs 显著降低了雌性动物对 2,4-DTBP 的吸收,而在共同暴露组中,2,4-DTBP 的肠道浓度在性别上没有差异。此外,雄鱼的肠道 pH 值较低,消化酶含量较高,而雌鱼的胆汁酸含量则显著增加。此外,共同暴露于 PS-NPs 会刺激雌鱼重塑微生物组成,从而有可能提高异种生物的降解,而阴性气单胞菌则会加重雄鱼的炎症。这些结果表明,在存在 PS-NPs 的情况下,雌鱼的肠道微环境可促进 2,4-DTBP 的解毒,同时加剧雄鱼的毒性。总之,这项研究表明,NPs-化学混合物的毒理学结果可能会因性别特异性生理和微生物群组成而改变,从而进一步加深了对环境风险评估和水生环境管理的理解。
{"title":"Polystyrene nanoplastics alter intestinal toxicity of 2,4-DTBP in a sex-dependent manner in zebrafish (Danio rerio).","authors":"Ruimin Liu, Huina Gao, Xuefang Liang, Jiye Zhang, Qingjian Meng, Yuchen Wang, Wei Guo, Christopher J Martyniuk, Jinmiao Zha","doi":"10.1016/j.jhazmat.2024.135585","DOIUrl":"10.1016/j.jhazmat.2024.135585","url":null,"abstract":"<p><p>Nanoplastics (NPs) and 2,4-di-tert-butylphenol (2,4-DTBP) are ubiquitous emerging environmental contaminants detected in aquatic environment. While the intestinal toxicity of 2,4-DTBP alone has been studied, its combined effects with NPs remain unclear. Herein, adult zebrafish were exposed to 80 nm polystyrene nanoplastics (PS-NPs) or/ and 2,4-DTBP for 28 days. With co-exposure of PS-NPs, impact of 2,4-DTBP on feeding capacity and intestinal histopathology was enhanced in males while attenuated in females. Addition of PS-NPs significantly decreased the uptake of 2,4-DTBP in females, while the intestinal concentrations of 2,4-DTBP were not different between the sexes in co-exposure groups. Furthermore, lower intestinal pH and higher contents of digestive enzymes were detected in male fish, while bile acid was significantly increased in co-exposed females. In addition, co-exposure of PS-NPs stimulated female fish to remodel microbial composition to potentially enhance xenobiotics degradation, while negative Aeromonas aggravated inflammation in males. These results indicated that in the presence of PS-NPs, the gut microenvironment in females can facilitate the detoxification of 2,4-DTBP, while exaggerating toxiciy in males. Overall, this study demonstrates that toxicological outcomes of NPs-chemical mixtures may be modified by sex-specific physiology and microbiota composition, furthering understanding for environmental risk assessment and management of aquatic environments.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135585"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142047722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Highly selective localized surface plasmon resonance sensor for selenium diagnosis in selenium-rich soybeans. 用于富硒大豆硒诊断的高选择性局部表面等离子体共振传感器。
Pub Date : 2024-10-05 Epub Date: 2024-08-22 DOI: 10.1016/j.jhazmat.2024.135632
Suyan Qiu, Yifan Dong, Xiren Yu, Qiushuang Ai, Lijuan Yuan, Li Zhang, Dawen Zhang

It is a challenge to determine selenium in acid aqueous for environmental monitoring and selenium-rich agricultural diagnosis. Herein, we developed a novel localized surface plasmon resonance (LSPR) sensor to detect Se(IV) ions based on the extraordinary laterals etching of gold nanorods (AuNRs). The etching started from the laterals in the low amount of Se(IV) ions, and accompanied by an apparent red shift of the longitudinal plasmon band (LPB), and then transformed to the tips etching with the upward of Se(IV) ions, the LPB band immediately shifted to the shorter wavelength. The red shift change (Δλ) of LPB band was utilized to quantitative analysis instead of blue shift or absorbance intensity, which gave a high selectivity for the proposed sensor. More importantly, this sensor could be performed in 0.1 mol/L of HCl solution, which achieved the seamlessly jointing with the pretreatment of complex samples, without time-consuming pH adjustment.Successful selenium detection was demonstrated in complex soybean samples that collected from the maturity after spraying organic chelated selenium at full flower period. The sensor provided a promising way to monitor and diagnose selenium in complex environmental samples and selenium-rich crops.

如何测定酸性水体中的硒以进行环境监测和富硒农业诊断是一项挑战。在此,我们开发了一种新型局部表面等离子体共振(LSPR)传感器,利用金纳米棒(AuNRs)的非凡侧向刻蚀来检测硒(IV)离子。在Se(IV)离子含量较低时,刻蚀从侧面开始,伴随着纵向等离子体波段(LPB)的明显红移,然后随着Se(IV)离子含量的上升转变为尖端刻蚀,LPB波段立即向较短波长移动。利用 LPB 波段的红移变化(Δλ)进行定量分析,而不是蓝移或吸光度,这就为拟议的传感器提供了高选择性。更重要的是,该传感器可在 0.1 mol/L 的盐酸溶液中使用,实现了与复杂样品预处理的无缝对接,无需费时调节 pH 值。该传感器为监测和诊断复杂环境样品和富硒作物中的硒含量提供了一种可行的方法。
{"title":"Highly selective localized surface plasmon resonance sensor for selenium diagnosis in selenium-rich soybeans.","authors":"Suyan Qiu, Yifan Dong, Xiren Yu, Qiushuang Ai, Lijuan Yuan, Li Zhang, Dawen Zhang","doi":"10.1016/j.jhazmat.2024.135632","DOIUrl":"10.1016/j.jhazmat.2024.135632","url":null,"abstract":"<p><p>It is a challenge to determine selenium in acid aqueous for environmental monitoring and selenium-rich agricultural diagnosis. Herein, we developed a novel localized surface plasmon resonance (LSPR) sensor to detect Se(IV) ions based on the extraordinary laterals etching of gold nanorods (AuNRs). The etching started from the laterals in the low amount of Se(IV) ions, and accompanied by an apparent red shift of the longitudinal plasmon band (LPB), and then transformed to the tips etching with the upward of Se(IV) ions, the LPB band immediately shifted to the shorter wavelength. The red shift change (Δλ) of LPB band was utilized to quantitative analysis instead of blue shift or absorbance intensity, which gave a high selectivity for the proposed sensor. More importantly, this sensor could be performed in 0.1 mol/L of HCl solution, which achieved the seamlessly jointing with the pretreatment of complex samples, without time-consuming pH adjustment.Successful selenium detection was demonstrated in complex soybean samples that collected from the maturity after spraying organic chelated selenium at full flower period. The sensor provided a promising way to monitor and diagnose selenium in complex environmental samples and selenium-rich crops.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135632"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved microbial-plant soil bioremediation of PAHs and heavy metal through in silico methods. 通过硅学方法改进多环芳烃和重金属的微生物-植物土壤生物修复。
Pub Date : 2024-10-05 Epub Date: 2024-08-14 DOI: 10.1016/j.jhazmat.2024.135524
Lei Zhao, Peixuan Sun, Jiaxuan Gao, Yunxiang Li, Qikun Pu, Cong Lyu, Wenjin Zhao

The combined pollution of polycyclic aromatic hydrocarbons (PAHs) and organic cadmium (Cd) in farmland soils, and the field controlling strategy need to be studied urgently. In this study, 5 PAHs, 5 Cd and 11 soil conditioners were selected to explore the co-exposure risk and remediation efficiency. Firstly, a significant combination Fl-alkylalkoxy cadmium was obtained using forward and reverse methods coupling variation coefficient methods (the combined pollution value was 0.173). Secondly, the interaction energy of microbial degradation / plant absorption of Fl under Cd stress, and microbial mineralization / plant absorption of alkylalkoxy cadmium under PAHs stress were characterized using factorial experimental design, molecular docking and molecular dynamics simulation. The combined pollution of alkylalkoxy cadmium and dialkyl cadmium, phenanthrene and Benzo [a] pyrene was significant (synergistic contribution rates were 17.58 % and 19.22 %, respectively). In addition, 6 soil conditioners with significant efficiency were selected to design Taguchi orthogonal experimental schemes, indicating the microbial degradation / mineralization and plant absorption were significantly effective (the maximum increase of remediation efficiency was 93.81 %) under the combinations (i.e., trratone, coumarol, fulvamic acid, potassium fertilizer and others, etc.). Finally, it was found that the soil conditioners affected the hydrophobic groups and forces, and the efficiency was proportional to the highest peak value and minimum distance in the RDF curve. This study identifies the risk characteristics of co-exposure of PAHs and Cd and screens effective soil conditioners, providing theoretical guidance for risk controlling.

农田土壤中多环芳烃(PAHs)和有机镉(Cd)的复合污染及田间控制策略亟待研究。本研究选取了 5 种多环芳烃、5 种镉和 11 种土壤调理剂,探讨其共同暴露风险和修复效率。首先,利用正向和反向耦合变异系数法得到了一个重要的Fl-烷基烷氧基镉组合(综合污染值为0.173)。其次,利用因子实验设计、分子对接和分子动力学模拟表征了镉胁迫下微生物降解/植物吸收 Fl 和多环芳烃胁迫下微生物矿化/植物吸收烷基烷氧基镉的相互作用能。烷基烷氧基镉与二烷基镉、菲和苯并[a]芘的联合污染显著(协同贡献率分别为 17.58 % 和 19.22 %)。此外,通过田口正交试验设计方案,选择了 6 种效果显著的土壤调理剂,结果表明,在三唑酮、香豆素、富乐菌素、钾肥等组合下,微生物降解/矿化和植物吸收效果显著(修复效率最大提高 93.81%)。最后,研究发现土壤改良剂会影响疏水基团和疏水作用力,其效率与 RDF 曲线的最高峰值和最小距离成正比。该研究明确了多环芳烃和镉共同暴露的风险特征,筛选出有效的土壤改良剂,为风险控制提供了理论指导。
{"title":"Improved microbial-plant soil bioremediation of PAHs and heavy metal through in silico methods.","authors":"Lei Zhao, Peixuan Sun, Jiaxuan Gao, Yunxiang Li, Qikun Pu, Cong Lyu, Wenjin Zhao","doi":"10.1016/j.jhazmat.2024.135524","DOIUrl":"10.1016/j.jhazmat.2024.135524","url":null,"abstract":"<p><p>The combined pollution of polycyclic aromatic hydrocarbons (PAHs) and organic cadmium (Cd) in farmland soils, and the field controlling strategy need to be studied urgently. In this study, 5 PAHs, 5 Cd and 11 soil conditioners were selected to explore the co-exposure risk and remediation efficiency. Firstly, a significant combination Fl-alkylalkoxy cadmium was obtained using forward and reverse methods coupling variation coefficient methods (the combined pollution value was 0.173). Secondly, the interaction energy of microbial degradation / plant absorption of Fl under Cd stress, and microbial mineralization / plant absorption of alkylalkoxy cadmium under PAHs stress were characterized using factorial experimental design, molecular docking and molecular dynamics simulation. The combined pollution of alkylalkoxy cadmium and dialkyl cadmium, phenanthrene and Benzo [a] pyrene was significant (synergistic contribution rates were 17.58 % and 19.22 %, respectively). In addition, 6 soil conditioners with significant efficiency were selected to design Taguchi orthogonal experimental schemes, indicating the microbial degradation / mineralization and plant absorption were significantly effective (the maximum increase of remediation efficiency was 93.81 %) under the combinations (i.e., trratone, coumarol, fulvamic acid, potassium fertilizer and others, etc.). Finally, it was found that the soil conditioners affected the hydrophobic groups and forces, and the efficiency was proportional to the highest peak value and minimum distance in the RDF curve. This study identifies the risk characteristics of co-exposure of PAHs and Cd and screens effective soil conditioners, providing theoretical guidance for risk controlling.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135524"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mapping and monitoring dense non-aqueous phase liquid source zone by fused surface and cross-borehole electrical resistivity tomography. 利用熔融表面和跨钻孔电阻率层析成像技术绘制和监测高密度非水相液源区。
Pub Date : 2024-10-05 Epub Date: 2024-08-22 DOI: 10.1016/j.jhazmat.2024.135618
Fansong Meng, Jinguo Wang, Yongsheng Zhao

Effective characterization of dense non-aqueous phase liquid (DNAPL) source zones is crucial for remediating polluted sites. DNAPL often reside as residuals or pools within high-permeability lenses and above impermeable layers due to soil heterogeneity, gravity, and capillary barriers. Given the high cost of drilling, electrical resistivity tomography (ERT) techniques-including surface ERT and cross-borehole ERT, are commonly used for DNAPL source zone mapping and monitoring. However, the low spatial resolution of ERT increases uncertainty in source zone investigations. This study proposes a method for improving DNAPL mapping and monitoring by fusing surface and cross-borehole ERT data. Sandbox experiments were conducted to simulate a heterogeneous DNAPL source zone, employing both ERT methods for static mapping and dynamic monitoring. Reflective light imaging (RLM) was used to visualize DNAPL migration and provide saturation data, allowing for the quantification of ERT's effectiveness in characterizing DNAPL distribution. The results indicate that individual ERT methods face significant challenges in DNAPL source zone mapping due to background interference. Surface ERT alone tends to underestimate the extent of deeper DNAPL source zones. However, fusing surface and cross-borehole ERT results in a complementary enhancement of vertical spatial resolution, thereby improving the characterization of DNAPL source zones. The fusion of static and time-lapse ERT data substantially enhances DNAPL source zone mapping and monitoring capabilities. By calculating the ratio of the ERT-monitored area to the actual area using resistivity change contours (5 %, 10 %, 15 %), it was found that fusing surface and cross-borehole ERT data improved monitoring resolution by 50.48 % compared to surface ERT alone and by 22.95 % compared to cross-borehole ERT. Principal component analysis (PCA) was effective in fusing time-lapse data, while the weighted average method (WAM) outperformed PCA for static resistivity data fusion.

有效确定致密非水相液体(DNAPL)源区的特征对于污染场地的修复至关重要。由于土壤的异质性、重力和毛细管障碍,DNAPL 通常以残留物或油池的形式存在于高渗透透镜内部和不透水层之上。由于钻探成本高昂,电阻率层析成像 (ERT) 技术(包括地表 ERT 和交叉钻孔 ERT)通常用于 DNAPL 源区绘图和监测。然而,ERT 的空间分辨率较低,增加了源区调查的不确定性。本研究提出了一种通过融合地面和跨钻孔 ERT 数据来改进 DNAPL 测绘和监测的方法。通过沙箱实验模拟了一个异质 DNAPL 源区,采用两种 ERT 方法进行静态绘图和动态监测。利用反射光成像(RLM)可视化 DNAPL 迁移并提供饱和度数据,从而量化 ERT 在描述 DNAPL 分布特征方面的有效性。结果表明,由于背景干扰,单个 ERT 方法在绘制 DNAPL 源区图时面临巨大挑战。单靠地表ERT往往会低估深层DNAPL源区的范围。然而,将地表和跨钻孔 ERT 融合在一起,可以互补地提高垂直空间分辨率,从而改善 DNAPL 源区的特征描述。静态和延时 ERT 数据的融合大大提高了 DNAPL 源区的绘图和监测能力。通过使用电阻率变化等值线(5%、10%、15%)计算 ERT 监测区域与实际区域的比率,发现与单独使用地面 ERT 相比,融合地面和跨钻孔 ERT 数据可提高监测分辨率 50.48%,与跨钻孔 ERT 相比,可提高 22.95%。主成分分析法(PCA)在融合延时数据方面很有效,而加权平均法(WAM)在融合静态电阻率数据方面则优于 PCA。
{"title":"Mapping and monitoring dense non-aqueous phase liquid source zone by fused surface and cross-borehole electrical resistivity tomography.","authors":"Fansong Meng, Jinguo Wang, Yongsheng Zhao","doi":"10.1016/j.jhazmat.2024.135618","DOIUrl":"10.1016/j.jhazmat.2024.135618","url":null,"abstract":"<p><p>Effective characterization of dense non-aqueous phase liquid (DNAPL) source zones is crucial for remediating polluted sites. DNAPL often reside as residuals or pools within high-permeability lenses and above impermeable layers due to soil heterogeneity, gravity, and capillary barriers. Given the high cost of drilling, electrical resistivity tomography (ERT) techniques-including surface ERT and cross-borehole ERT, are commonly used for DNAPL source zone mapping and monitoring. However, the low spatial resolution of ERT increases uncertainty in source zone investigations. This study proposes a method for improving DNAPL mapping and monitoring by fusing surface and cross-borehole ERT data. Sandbox experiments were conducted to simulate a heterogeneous DNAPL source zone, employing both ERT methods for static mapping and dynamic monitoring. Reflective light imaging (RLM) was used to visualize DNAPL migration and provide saturation data, allowing for the quantification of ERT's effectiveness in characterizing DNAPL distribution. The results indicate that individual ERT methods face significant challenges in DNAPL source zone mapping due to background interference. Surface ERT alone tends to underestimate the extent of deeper DNAPL source zones. However, fusing surface and cross-borehole ERT results in a complementary enhancement of vertical spatial resolution, thereby improving the characterization of DNAPL source zones. The fusion of static and time-lapse ERT data substantially enhances DNAPL source zone mapping and monitoring capabilities. By calculating the ratio of the ERT-monitored area to the actual area using resistivity change contours (5 %, 10 %, 15 %), it was found that fusing surface and cross-borehole ERT data improved monitoring resolution by 50.48 % compared to surface ERT alone and by 22.95 % compared to cross-borehole ERT. Principal component analysis (PCA) was effective in fusing time-lapse data, while the weighted average method (WAM) outperformed PCA for static resistivity data fusion.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135618"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reply to 'The causal relationship between long-term exposure to ambient fine particulate matter and cognitive performance: Insights from Mendelian randomization'. 回复 "长期暴露于环境微粒物质与认知能力之间的因果关系:孟德尔随机化的启示"。
Pub Date : 2024-10-05 Epub Date: 2024-08-22 DOI: 10.1016/j.jhazmat.2024.135622
Kejia Hu, Jin Qi, Yao Yao

Li et al. [1] have commented on our recent paper investigating the association between exposure to fine particulate matter (PM2.5) constituents and the risk of cognitive impairment [2]. They provided a Mendelian randomization (MR) analysis using large-scale genome-wide association study (GWAS) datasets from the European population, confirming a causal relationship between PM2.5 exposure and cognitive performance. In our reply, we employed three causal inference models, including a generalized propensity score (GPS) adjusted Cox model, an inverse-probability weights (IPW) weighted Cox model, and a trimmed IPW-weighted Cox model, to confirm the relationship of PM2.5 and cognitive impairment in our study cohort.

Li等人[1]对我们最近发表的研究细颗粒物(PM2.5)成分暴露与认知障碍风险之间关系的论文[2]发表了评论。他们利用来自欧洲人群的大规模全基因组关联研究(GWAS)数据集进行了孟德尔随机化(MR)分析,证实了 PM2.5 暴露与认知能力之间的因果关系。在我们的回答中,我们采用了三种因果推理模型,包括广义倾向得分(GPS)调整的 Cox 模型、反概率权重(IPW)加权的 Cox 模型和经过修剪的 IPW 加权的 Cox 模型,来证实我们的研究队列中 PM2.5 与认知障碍之间的关系。
{"title":"Reply to 'The causal relationship between long-term exposure to ambient fine particulate matter and cognitive performance: Insights from Mendelian randomization'.","authors":"Kejia Hu, Jin Qi, Yao Yao","doi":"10.1016/j.jhazmat.2024.135622","DOIUrl":"10.1016/j.jhazmat.2024.135622","url":null,"abstract":"<p><p>Li et al. [1] have commented on our recent paper investigating the association between exposure to fine particulate matter (PM<sub>2.5</sub>) constituents and the risk of cognitive impairment [2]. They provided a Mendelian randomization (MR) analysis using large-scale genome-wide association study (GWAS) datasets from the European population, confirming a causal relationship between PM<sub>2.5</sub> exposure and cognitive performance. In our reply, we employed three causal inference models, including a generalized propensity score (GPS) adjusted Cox model, an inverse-probability weights (IPW) weighted Cox model, and a trimmed IPW-weighted Cox model, to confirm the relationship of PM<sub>2.5</sub> and cognitive impairment in our study cohort.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135622"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Waste control by waste: A new approach for antibiotic removal and metal reuse from livestock wastewater using ascorbic acid-enhanced CaO2/Cu(II) system. 以废治废:利用抗坏血酸强化 CaO2/Cu(II)系统从畜牧废水中去除抗生素和金属再利用的新方法。
Pub Date : 2024-10-05 Epub Date: 2024-08-23 DOI: 10.1016/j.jhazmat.2024.135496
Rongfa Zhang, Song Xia, Wenyue Yu, Guanglan Di, Jifei Hou, Xuede Li, Mingbao Feng

Aiming at the coexistence of antibiotics and Cu(II) in livestock wastewater, a novelty strategy for the simultaneous removal of antibiotics and Cu ions by in-situ utilization of Cu(II) (i.e., CP/Cu(II) and CP/Cu(II)/ascorbic acid (AA) systems) was proposed. The removal rate of florfenicol (FF) in the CP/Cu(II)/AA system was 6.9 times higher than that of the CP/Cu(II) system. CP/Cu(II)/AA system was also effective in removing antibiotics from real livestock tailwater. Simultaneously, the removal of Cu ions in CP/Cu(II) and CP/Cu(II)/AA systems could reach 54.5 % and 15.7 %, respectively. The added AA could significantly enhance the antibiotics degradation but inhibit the Cu ions removal. HO, O2•-, Cu(III), and C-R were detected in the CP/Cu(II)/AA system, in which HO was confirmed as the predominant contributor for FF degradation, and Cu(III) and C-R also participated in FF elimination. The role of AA could accelerate HO production and Cu(I)/Cu(II)/Cu(III) cycle, and form C-R. The degradation products and pathways of FF in the CP/Cu(II)/AA system were proposed and the toxicity of the degradation products was evaluated by the toxicity analysis software (T.E.S.T). The results of this work suggest that without introducing complex catalysts, the feasibility of in-situ utilization of Cu(II) inherently or artificially introduced in livestock wastewater activating CP for antibiotic degradation and Cu ions removal was verified.

针对畜牧业废水中抗生素和 Cu(II)共存的问题,提出了一种通过原位利用 Cu(II)(即 CP/Cu(II) 和 CP/Cu(II)/ascorbic acid (AA) 系统)同时去除抗生素和 Cu 离子的新策略。CP/Cu(II)/AA 系统对氟苯尼考(FF)的去除率是 CP/Cu(II) 系统的 6.9 倍。CP/Cu(II)/AA 系统也能有效去除实际牲畜尾水中的抗生素。同时,CP/Cu(II) 和 CP/Cu(II)/AA 系统对铜离子的去除率分别达到 54.5% 和 15.7%。添加的 AA 能明显促进抗生素的降解,但抑制了铜离子的去除。CP/Cu(II)/AA体系中检测到了HO-、O2-、Cu(III)和-C-R,其中HO-被证实是FF降解的主要因素,Cu(III)和-C-R也参与了FF的消除。AA 的作用可加速 HO- 的产生和 Cu(I)/Cu(II)/Cu(III) 循环,并形成 -C-R。提出了 FF 在 CP/Cu(II)/AA 系统中的降解产物和途径,并通过毒性分析软件(T.E.S.T)对降解产物的毒性进行了评估。研究结果表明,在不引入复合催化剂的情况下,验证了原位利用畜禽废水中固有或人工引入的 Cu(II) 活化 CP 进行抗生素降解和 Cu 离子去除的可行性。
{"title":"Waste control by waste: A new approach for antibiotic removal and metal reuse from livestock wastewater using ascorbic acid-enhanced CaO<sub>2</sub>/Cu(II) system.","authors":"Rongfa Zhang, Song Xia, Wenyue Yu, Guanglan Di, Jifei Hou, Xuede Li, Mingbao Feng","doi":"10.1016/j.jhazmat.2024.135496","DOIUrl":"10.1016/j.jhazmat.2024.135496","url":null,"abstract":"<p><p>Aiming at the coexistence of antibiotics and Cu(II) in livestock wastewater, a novelty strategy for the simultaneous removal of antibiotics and Cu ions by in-situ utilization of Cu(II) (i.e., CP/Cu(II) and CP/Cu(II)/ascorbic acid (AA) systems) was proposed. The removal rate of florfenicol (FF) in the CP/Cu(II)/AA system was 6.9 times higher than that of the CP/Cu(II) system. CP/Cu(II)/AA system was also effective in removing antibiotics from real livestock tailwater. Simultaneously, the removal of Cu ions in CP/Cu(II) and CP/Cu(II)/AA systems could reach 54.5 % and 15.7 %, respectively. The added AA could significantly enhance the antibiotics degradation but inhibit the Cu ions removal. HO<sup>•</sup>, O<sub>2</sub><sup>•-</sup>, Cu(III), and <sup>•</sup>C-R were detected in the CP/Cu(II)/AA system, in which HO<sup>•</sup> was confirmed as the predominant contributor for FF degradation, and Cu(III) and <sup>•</sup>C-R also participated in FF elimination. The role of AA could accelerate HO<sup>•</sup> production and Cu(I)/Cu(II)/Cu(III) cycle, and form <sup>•</sup>C-R. The degradation products and pathways of FF in the CP/Cu(II)/AA system were proposed and the toxicity of the degradation products was evaluated by the toxicity analysis software (T.E.S.T). The results of this work suggest that without introducing complex catalysts, the feasibility of in-situ utilization of Cu(II) inherently or artificially introduced in livestock wastewater activating CP for antibiotic degradation and Cu ions removal was verified.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135496"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interference-resistant gold nanoparticle assay for detecting Enterococcus in fresh and marine waters. 用于检测淡水和海水中肠球菌的抗干扰金纳米粒子测定法。
Pub Date : 2024-10-05 Epub Date: 2024-08-09 DOI: 10.1016/j.jhazmat.2024.135463
Yunsoo Chang, Ji-Hyeon Sung, Seung-Woo Lee, Eun-Hee Lee

Enterococci are common indicators of fecal contamination and are used to assess the quality of fresh and marine water, sand, soil, and sediment. However, samples collected from these environments contain various cells and other factors that can interfere with the assays used to detect enterococci. We developed a novel assay for the sensitive and specific detection of enterococci that is resistant to interference from other cells and environmental factors. Our interference-resistant assay used 30-nm gold nanoparticles (AuNPs), streptavidin, and a biotinylated Enterococcus antibody. Enterococci inhibited the interaction between streptavidin and biotin and led to the disaggregation of AuNPs. The absence of enterococci led to the aggregation of AuNPs, and this difference was easily detected by spectrophotometry. This interference-resistant AuNP assay was able to detect whole cells of Enterococcus in the range of 10 to 107 CFU/mL within 3 h, had high specificity for enterococci, and was unaffected by the presence of other intestinal bacteria, such as Escherichia coli. Our examination of fresh and marine water samples demonstrated no interference from other cells or environmental factors. The interference-resistant AuNP assay described here has the potential to be used as a rapid, simple, and effective method for monitoring enterococci in diverse environmental samples.

肠球菌是粪便污染的常见指标,用于评估淡水和海水、沙子、土壤和沉积物的质量。然而,从这些环境中采集的样本含有各种细胞和其他因素,会干扰用于检测肠球菌的检测方法。我们开发了一种新型检测方法,用于灵敏、特异地检测肠球菌,该方法可抵抗其他细胞和环境因素的干扰。我们的抗干扰测定使用了 30 纳米金颗粒 (AuNPs)、链霉亲和素和生物素化的肠球菌抗体。肠球菌抑制了链霉亲和素和生物素之间的相互作用,导致 AuNPs 分解。没有肠球菌则会导致 AuNPs 聚合,这种差异很容易通过分光光度法检测出来。这种抗干扰 AuNP 检测法能够在 3 小时内检测到 10 至 107 CFU/mL 的肠球菌全细胞,对肠球菌具有高度特异性,并且不受大肠杆菌等其他肠道细菌存在的影响。我们对淡水和海水样本的检测结果表明,没有受到其他细胞或环境因素的干扰。本文介绍的抗干扰 AuNP 检测法有望成为一种快速、简单、有效的方法,用于监测各种环境样本中的肠球菌。
{"title":"Interference-resistant gold nanoparticle assay for detecting Enterococcus in fresh and marine waters.","authors":"Yunsoo Chang, Ji-Hyeon Sung, Seung-Woo Lee, Eun-Hee Lee","doi":"10.1016/j.jhazmat.2024.135463","DOIUrl":"10.1016/j.jhazmat.2024.135463","url":null,"abstract":"<p><p>Enterococci are common indicators of fecal contamination and are used to assess the quality of fresh and marine water, sand, soil, and sediment. However, samples collected from these environments contain various cells and other factors that can interfere with the assays used to detect enterococci. We developed a novel assay for the sensitive and specific detection of enterococci that is resistant to interference from other cells and environmental factors. Our interference-resistant assay used 30-nm gold nanoparticles (AuNPs), streptavidin, and a biotinylated Enterococcus antibody. Enterococci inhibited the interaction between streptavidin and biotin and led to the disaggregation of AuNPs. The absence of enterococci led to the aggregation of AuNPs, and this difference was easily detected by spectrophotometry. This interference-resistant AuNP assay was able to detect whole cells of Enterococcus in the range of 10 to 10<sup>7</sup> CFU/mL within 3 h, had high specificity for enterococci, and was unaffected by the presence of other intestinal bacteria, such as Escherichia coli. Our examination of fresh and marine water samples demonstrated no interference from other cells or environmental factors. The interference-resistant AuNP assay described here has the potential to be used as a rapid, simple, and effective method for monitoring enterococci in diverse environmental samples.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"478 ","pages":"135463"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142038105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of hazardous materials
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1