Carbohydrate Research最新文献_第10页

Yeast mannan: A comprehensive functional analysis from structural characteristics to biological activity 酵母甘露聚糖：从结构特征到生物活性的综合功能分析。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-25 DOI: 10.1016/j.carres.2025.109715

Qiang Wei

Yeast mannan, an essential polysaccharide derived from the cell walls of yeast, has garnered significant interest in recent years owing to its diverse biological functions and potential health benefits. This review intends to thoroughly explore the structural characteristics of yeast mannan, elucidating the manner in which its unique molecular architecture affects its functional properties. Recent studies highlight its multiple health contributions, including notable antioxidant effects, immunomodulatory functions, and the enhancement of gastrointestinal health. However, challenges remain in fully elucidating the relationship between structure and function, particularly regarding the differential regulatory influences of specific types of glycosidic bonds on immunomodulatory activities, as well as improving extraction and application techniques for industrial use. By integrating the most recent research findings, this article aims to emphasize the bioactive potential of yeast mannan and examine its prospective applications in nutraceuticals and functional foods. Additionally, the review identifies existing knowledge gaps and suggests avenues for future research aimed at optimizing its therapeutic potential, thereby establishing a scientific basis for both academic research and commercial application. In particular, a comprehensive understanding of the structure-activity relationship (SAR) related to yeast mannan, especially the correlation between glycosidic bond specificity and biological efficacy, remains critical. Furthermore, assessing the toxicological risks associated with mannan derivatives is another vital yet often neglected aspect necessary for the safe industrial application of these compounds.

酵母甘露聚糖是一种从酵母细胞壁中提取的重要多糖，近年来由于其多种生物功能和潜在的健康益处而引起了人们的极大兴趣。本文旨在深入探讨酵母甘露聚糖的结构特征，阐明其独特的分子结构对其功能特性的影响。最近的研究强调了其多种健康贡献，包括显著的抗氧化作用、免疫调节功能和增强胃肠道健康。然而，在充分阐明结构与功能之间的关系方面仍然存在挑战，特别是在特定类型的糖苷键对免疫调节活性的不同调节影响方面，以及改进工业用途的提取和应用技术方面。本文结合近年来的研究成果，重点介绍了酵母甘露聚糖的生物活性潜力，并对其在营养保健品和功能食品中的应用前景进行了展望。此外，本综述还指出了现有的知识差距，并提出了旨在优化其治疗潜力的未来研究途径，从而为学术研究和商业应用奠定了科学基础。特别是，全面了解酵母甘露聚糖的结构-活性关系（SAR），特别是糖苷键特异性与生物功效之间的关系，仍然是至关重要的。此外，评估与甘露聚糖衍生物相关的毒理学风险是这些化合物安全工业应用所必需的另一个重要但经常被忽视的方面。

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引用次数: 0

Human O-GlcNAc transferase substrate recognition via MALDI-TOF MS quantification of peptide glycosylation 人O-GlcNAc转移酶底物识别通过MALDI-TOF质谱定量肽糖基化。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-24 DOI: 10.1016/j.carres.2025.109713

Jun Bian, Yaqing Wang, Li Liu, Josef Voglmeir

Human O-GlcNAc transferase (hOGT) modifies serine/threonine residues on cytosolic and nuclear proteins, but predictive rules for substrate recognition are incomplete. Here, we combine MALDI-TOF MS quantification of intact peptides, conversion-based sequence logos, and molecular docking to elucidate recognition patterns. Testing a panel of synthetic peptides (7–25 residues) yielded O-GlcNAcylation from 0 % to 100 %. Stereoselective glycosylation of EA2-R produced β-O-GlcNAc (hOGT/UDP-GlcNAc), α-O-GlcNAc, and α-O-GalNAc (ppGalNAc-T2/UDP-GlcNAc or UDP-GalNAc), enabling comparative studies. Tiered analysis (≥30 %, <30 %, 0 % conversion) highlighted trends: efficient substrates prefer Pro at −2/-3, Val/Ser at −1, Ser at +1, Ala at +2, and Ser acceptors; lower groups feature reduced Pro/Val, increased acids; non-substrates show Gly at −2/-1 and poor +1/+2 residues. Targeted variants tuned reactivity (e.g., Val near −3 achieved 100 % for CKII; distal edits affected CRYAA). Docking revealed productive contacts in reactive peptides. These findings provide design rules for O-GlcNAc assays and a framework for peptide substrate engineering.

人类O-GlcNAc转移酶（hOGT）修饰细胞质和核蛋白上的丝氨酸/苏氨酸残基，但底物识别的预测规则尚不完整。在这里，我们结合了完整肽的MALDI-TOF质谱定量、基于转换的序列标识和分子对接来阐明识别模式。测试一组合成肽（7-25个残基）的o - glcn酰化率从0%到100%。EA2-R的立体选择性糖基化产生β-O-GlcNAc （hOGT/UDP-GlcNAc）、α-O-GlcNAc和α-O-GalNAc (ppGalNAc-T2/UDP-GlcNAc或UDP-GalNAc)，可以进行比较研究。分层分析(≥30%；

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引用次数: 0

Reuterin integrated as dynamic imine crosslinks strengthens creatine modified chitosan hydrogel and boosts antibacterial efficacy 芦黄素作为动态亚胺交联体增强肌酸修饰壳聚糖水凝胶的抗菌效果。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-24 DOI: 10.1016/j.carres.2025.109716

Yongsheng Zheng , Kai You , Cuiping Guo , Zhijie Geng , Yuwei Gao , Zhaoming Liu , Dong Yang , Huilong Guo , Jun Huang

We report a hydrogel platform that integrates a natural antimicrobial as part of the network rather than as a releasable additive. Creatine was first grafted onto methacrylated hydroxybutyl chitosan to obtain an injectable, thermoresponsive, and photocurable backbone (HBC_m_Cre). Reuterin (Reu) was subsequently incorporated through dynamic imine (Schiff-base) coupling, functioning as structural crosslinks that both reinforce the network and sustain local antimicrobial activity. ¹H NMR and FTIR confirmed the successful synthesis. The resulting HBC_m_Cre/Reu hydrogel exhibited smooth injectability, rapid photocuring, and stepwise enhancement in mechanical performance (HBC: 0.45 ± 0.09 N, HBC_m_Cre: 1.27 ± 0.08 N, HBC_m_Cre/Reu: 1.96 ± 0.09 N), indicating that the integration of Reu as a network-forming unit significantly strengthens the gel architecture. The system maintained high biosafety (NIH 3T3 viability >95 %, hemolysis <2 % at 10 mg/mL) and showed potent antibacterial activity, achieving >95 % killing of E. coli and S. aureus and effective biofilm disruption. In vivo rat tail-amputation experiments further demonstrated efficient bleeding control, with creatine grafting markedly reducing bleeding time and blood loss, while Reu primarily contributed to mechanical reinforcement and antibacterial protection rather than direct hemostatic enhancement. These findings establish a distinct structural design strategy where a natural metabolite and antimicrobial are co-integrated into a single hydrogel network, providing a robust and multifunctional platform for potential application in infection-resistant wound care.

我们报告了一个水凝胶平台，将天然抗菌剂作为网络的一部分，而不是作为可释放的添加剂。首先将肌酸接枝到甲基丙烯酸羟丁基壳聚糖上，获得可注射的、热响应的、光固化的骨架（HBC_m_Cre）。Reuterin （Reu）随后通过动态亚胺（希夫碱）偶联被纳入，作为结构交联，既加强了网络，又维持了局部抗菌活性。1H NMR和FTIR证实了合成的成功。制备的HBC_m_Cre/Reu水凝胶具有可注射性好、光固化速度快、力学性能逐步增强的特点（HBC: 0.45±0.09 N, HBC_m_Cre: 1.27±0.08 N, HBC_m_Cre/Reu: 1.96±0.09 N），表明Reu作为网络形成单元的整合显著增强了凝胶结构。该系统具有较高的生物安全性（NIH 3T3存活率为95%），溶血率为95%，可杀灭大肠杆菌和金黄色葡萄球菌，并能有效破坏生物膜。体内大鼠截尾实验进一步证明了有效的止血控制，肌酸移植明显减少出血时间和出血量，而Reu主要起到机械加固和抗菌保护作用，而不是直接增强止血作用。这些发现建立了一种独特的结构设计策略，其中天然代谢物和抗菌剂共同集成到单个水凝胶网络中，为抗感染伤口护理的潜在应用提供了一个强大的多功能平台。

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引用次数: 0

Preparation and characterization of chitosan/citric acid composite film and its application in blueberry preservation 壳聚糖/柠檬酸复合膜的制备、表征及其在蓝莓保鲜中的应用

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-24 DOI: 10.1016/j.carres.2025.109714

Lan Wang, Jiaxin Ma, Xuya Zhang, Shenghui He, Yiheng Duan

Chitosan (CS) is widely utilized as a natural preservative for fruits and vegetables; however, its practical application is constrained by inferior mechanical properties and low water resistance. In this study, citric acid (CA) was incorporated into the chitosan matrix to leverage its multifunctional carboxyl groups and inherent antibacterial properties, aiming to enhance both the mechanical and antibacterial performance of the resulting composite films. The structure of the CS/CA composite films was characterized using Fourier transform infrared spectroscopy (FT-IR), ¹H nuclear magnetic resonance (¹H NMR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The physicochemical properties, antimicrobial activity, and potential for blueberry preservation of CS/CA films with varying CA content were systematically evaluated. Results indicated that the incorporation of CA induced cross-linking of CS chains via hydrogen bonding and amidation, leading to significant improvements in optical transparency, thermal stability, and hydrophilicity. The mechanical properties of the composite films were remarkably enhanced, with the maximum tensile strength increasing by up to fourfold compared to that of pure CS films. Composite films, particularly CS/CA4–CS/CA6, exhibited strong antibacterial effects against S. aureus and E. coli. Preservation tests on blueberries demonstrated the superior efficacy of the CS/CA composite films, especially CS/CA7, which showed optimal performance with only 5.6 % weight loss, maintained firmness at 228.3 g, the highest anthocyanin retention, and a 46.75 % reduction in decay rate compared to the control. These findings indicate that CS/CA composite films are promising environmentally friendly materials for active food packaging applications.

壳聚糖（CS）是一种广泛应用于果蔬的天然防腐剂；然而，其实际应用受到较差的力学性能和较低的耐水性的限制。本研究将柠檬酸（CA）加入到壳聚糖基质中，利用其多功能羧基和固有的抗菌性能，旨在提高复合膜的机械性能和抗菌性能。采用傅里叶变换红外光谱（FT-IR）、1H核磁共振（1H NMR）、x射线衍射（XRD）和扫描电镜（SEM）对CS/CA复合膜的结构进行了表征。系统评价了不同CA含量CS/CA膜的理化性质、抑菌活性和保存蓝莓的潜力。结果表明，CA的掺入诱导CS链通过氢键和酰胺化发生交联，从而显著改善了CS链的光学透明度、热稳定性和亲水性。复合膜的力学性能得到了显著提高，最大抗拉强度比纯CS膜提高了4倍。复合膜，特别是CS/ CA4-CS /CA6对金黄色葡萄球菌和大肠杆菌具有较强的抗菌作用。对蓝莓的保鲜试验表明，CS/CA复合膜的保鲜效果较好，其中CS/CA7的保鲜效果最佳，其蓝莓质量仅下降5.6%，硬度保持在228.3 g，花青素保有量最高，腐烂率比对照降低46.75%。这些研究结果表明，CS/CA复合薄膜是一种很有前途的环保材料，可用于活性食品包装。

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引用次数: 0

Synthesis of pullulan derivatives: Postharvest application in fruit preservation 普鲁兰衍生物的合成：采后水果保鲜的应用

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-22 DOI: 10.1016/j.carres.2025.109711

Fabian Hernandez-Tenorio , Catalina Giraldo-Estrada

The preservation of postharvest fruit is a major challenge in contributing to food security. Biopolymer-based coatings offer sustainable alternatives to reduce postharvest losses. This study aimed to evaluate the effect of pullulan (PU) oxidation and acetylation on its physicochemical properties and ability to preserve fruits postharvest quality. The derivatives obtained by chemical modification were pullulan dialdehyde (OxP) and pullulan acetate (PA), respectively. The degree of substitution (DS) was highest for OxP1 and PA1, with values of 36.8 % and 2.8 (no unit), respectively. Spectroscopic analysis revealed characteristic signals corresponding to aldehyde groups in the OxP derivatives and to acetyl groups in PA. Thermal analysis showed that PA increased thermal stability compared to PU (T_d of 238.8 °C). Conversely, OxPs showed lower values than PU. OxP compounds increased hydrophilicity compared to PU, whereas PA exhibited hydrophobic behavior. This effect is attributed to aldehyde groups promoting hydrogen bond formation with water, while acetyl groups reduce it. Water vapor and oxygen permeability were higher in PU, in contrast to OxP and PA. For mechanical properties, OxP3, the least oxidized compound, showed superior mechanical performance compared to PU, OxP1, OxP2, and PA. PA coatings resulted in the lowest ripening index in both blueberries and strawberries compared to the control and PU. The preservation of the sensory quality of the fruits was superior in OxP1, unlike PA1. Therefore, PU derivatives are a promising option to extend fruit postharvest quality.

采后水果的保存是促进粮食安全的一项重大挑战。基于生物聚合物的涂料提供了可持续的替代方案，以减少采收后的损失。本研究旨在探讨普鲁兰（PU）氧化和乙酰化处理对其理化性质和果实采后品质保藏能力的影响。化学改性得到的衍生物分别为普鲁兰双醛（OxP）和普鲁兰醋酸酯（PA）。OxP1和PA1的取代度（DS）最高，分别为36.8%和2.8（无单位）。光谱分析发现OxP衍生物的特征信号与醛基团和PA的乙酰基相对应。热分析表明，与PU相比，PA提高了热稳定性（Td为238.8℃）。相反，oxp值低于PU值。与PU相比，OxP化合物的亲水性增强，而PA则表现出疏水行为。这种效应是由于醛促进了与水的氢键形成，而乙酰基则减少了它。PU的水蒸气渗透性和透氧性高于OxP和PA。在力学性能方面，氧化程度最低的化合物OxP3与PU、OxP1、OxP2和PA相比，表现出更优异的力学性能。与对照和PU相比，PA处理蓝莓和草莓的成熟指数最低。与PA1不同，OxP1对果实感官品质的保存效果更好。因此，聚氨酯衍生物是延长水果采后品质的一个有希望的选择。

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引用次数: 0

Glycosylation using diethyl phosphite donor activated by edible polyphenol ellagic acid 食用聚酚鞣花酸活化亚磷酸二乙酯供体糖基化

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-22 DOI: 10.1016/j.carres.2025.109712

Hidenari Shigeta, Ayato Watanabe, Chikako Shiraishi, Daisuke Takahashi, Kazunobu Toshima

The glycosylation reactions of diethyl phosphite donor with various alcohol acceptors were examined using ellagic acid, an edible polyphenol, as an activator. These reactions proceeded smoothly under mild conditions to afford the corresponding glycosides in high yields. Notably, this method represents the first use of ellagic acid for the activation of phosphite donor and was applicable to a broad range of alcohol acceptors.

以食用多酚鞣花酸为活化剂，研究了亚磷酸二乙酯供体与不同醇受体的糖基化反应。这些反应在温和的条件下顺利进行，以高收率获得相应的糖苷。值得注意的是，该方法是首次使用鞣花酸活化亚磷酸酯供体，适用于广泛的醇受体。

引用次数: 0

First total synthesis of cyanopterin, a pterin glycoside isolated from a cyanobacterium 首次合成了从一种蓝藻菌中分离出来的蝶呤苷

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-21 DOI: 10.1016/j.carres.2025.109710

Tadashi Hanaya, Yuta Maeda, Kazumasa Ejiri

The first total synthesis and structural identification of cyanopterin, a pterin glycoside isolated from the cyanobacterium Synechocystis sp. PCC 6803, has been accomplished. The synthesis was achieved by convergent coupling of three key derivatives: d-glucuronate, d-galactose, and 6-hydroxymethylpterin. An α-selective glycosylation enabled efficient construction of the glucuronate–galactose disaccharide, while subsequent β-exclusive glycosylation with the 6-hydroxymethylpterin derivative furnished the desired pterin–disaccharide glycoside. Final deprotection provided cyanopterin in its natural form, allowing confirmation of its precise structure.

本文首次合成并鉴定了从藻胞菌中分离得到的一种蝶呤苷类化合物蓝翅素。通过三个关键衍生物：d-葡萄糖醛酸盐、d-半乳糖和6-羟甲基蝶呤的聚合偶联实现了合成。α-选择性糖基化可以有效地构建葡萄糖醛酸-半乳糖双糖，而随后与6-羟甲基蝶呤衍生物进行β-特异性糖基化可以得到所需的蝶呤-双糖糖苷。最后的去保护提供了自然形态的蓝翅虫素，从而确认了其精确的结构。

引用次数: 0

Comparison of the physical and chemical features of composites produced from textile waste and cellulose plants 纺织废料与纤维素植物合成复合材料的理化特性比较。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-21 DOI: 10.1016/j.carres.2025.109706

Norbert Lipka , Paulina Żarnowiec , Karolina Soja , Wiesław Kaca

One of the most pressing unresolved environmental challenges is the massive accumulation of unrecycled textile waste. In this study, we demonstrate that reinforcing recycled textile materials with plant-based cellulose fibers can yield functional composite materials. The following natural fibers were investigated: sisal, cotton, linen, coconut, wood, abaca, jute, and hemp. Both the textiles and plant fibers were ground into fragments ranging from 2 to 15 mm in length. Plant–textile composites were produced using an extruder at temperatures up to 195 °C, allowing for the plasticization of the polymer components in the textile waste. Plant fibers were incorporated into the recycled textile matrix at concentrations ranging from 3 % to 6 % by weight. A total of 19 different composite samples were fabricated and tested for their mechanical properties, including tensile strength, flexural strength, and compressive resistance.

FTIR spectra of all cellulose fibers exhibited characteristic cellulose absorption bands, with notable differences in intensity and distribution. The O–H stretching band (3600–3200 cm⁻¹), indicative of hydrogen bonding within cellulose, varied significantly across the different fiber types. SEM micrographs revealed that the surface of the sisal-reinforced composite was relatively smooth and uniform, while the fiberglass composite exhibited a rough and crinkled surface morphology.

In conclusion, composites made from recycled textiles and plant-based fibers represent a promising class of eco-friendly materials with potential as sustainable alternatives to conventional composites.

一个最紧迫的尚未解决的环境挑战是大量堆积的不可回收的纺织废料。在这项研究中，我们证明了用植物基纤维素纤维增强再生纺织材料可以产生功能性复合材料。研究了以下天然纤维：剑麻、棉花、亚麻、椰子、木材、abaca、黄麻和大麻。纺织品和植物纤维都被磨成2至15毫米长的碎片。植物-纺织复合材料是在195°C的温度下使用挤出机生产的，允许纺织废料中的聚合物成分塑化。植物纤维以重量3%至6%的浓度掺入回收的纺织基质中。共制备了19种不同的复合材料样品，并测试了它们的力学性能，包括拉伸强度、弯曲强度和抗压性。所有纤维素纤维的FTIR光谱均表现出特有的纤维素吸收带，其强度和分布有显著差异。O-H拉伸带（3600-3200 cm-1）在不同纤维类型中差异显著，表明纤维素内部的氢键。SEM显微形貌显示，剑麻增强复合材料表面相对光滑均匀，而玻璃纤维增强复合材料表面形貌粗糙、皱褶。总之，由回收纺织品和植物纤维制成的复合材料代表了一类有前途的环保材料，具有替代传统复合材料的可持续发展潜力。

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引用次数: 0

Resveratrol-loaded nanoparticles based on the self-assembly behavior of Sparassis latifolia polysaccharides: Preparation, characterization, release behavior under in vitro digestion and synergistic hypoglycemic effects 白藜芦醇负载纳米粒子的自组装行为：制备、表征、体外消化释放行为和协同降糖作用。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-20 DOI: 10.1016/j.carres.2025.109707

Pengya Du , Shuyu Bi , Fangming Cui , Lixia He , Jiangyan Hou , Jinling Cao , Yanfen Cheng , Feier Cheng , Wenfei Zhao , Cuiping Feng , Shaojun Yun

Resveratrol (Res) is a naturally occurring polyphenolic compound exhibiting a wide range of biological activities. However, its application is limited due to the instability, poor water solubility, and low bioavailability. Therefore, this study aimed to prepare Sparassis latifolia polysaccharides (SLPs) nanoparticles loaded with Res (SLPs-Res NPs) through the self-assembly behavior of SLPs. Our findings revealed that SLPs underwent a conformational transition from triple helix to single strand when exposed to varying concentrations of dimethyl sulfoxide (DMSO). By virtue of this self-assembly characteristic, the spherical or subspherical nanoparticles loaded with Res were successfully fabricated. The optimal preparation conditions included an 80 % volume ratio of DMSO, a mass ratio of Res to SLPs at 1:32, and magnetic stirring for 2 h. Under these conditions, the average particle size of SLPs-Res NPs was 230.4 ± 7.8 nm, and the encapsulation efficiency of Res reached (66.69 ± 0.10)%. Hydrogen bonding and hydrophobic forces were involved in the formation of nanoparticles. SLP-Res NPs demonstrated enhanced photothermal stability and desirable sustained-release properties. It also exerted significant inhibitory effects on α-glucosidase activity in vitro and glucose-lowering effects in mice during glucose tolerance tests. This study presents an approach for delivering Res using self-assembled SLPs, demonstrating their potential as effective functional carriers for the encapsulation of hydrophobic bioactive molecules.

白藜芦醇是一种天然存在的多酚类化合物，具有广泛的生物活性。然而，由于不稳定、水溶性差、生物利用度低，其应用受到限制。因此，本研究旨在通过木犀草多糖（SLPs）的自组装行为制备载Res纳米粒子（SLPs-Res NPs）。我们的研究结果表明，当暴露于不同浓度的二甲亚砜（DMSO）时，slp经历了从三螺旋到单链的构象转变。利用这种自组装特性，成功制备了负载稀土的球形或亚球形纳米颗粒。最佳制备条件为：DMSO体积比为80%，Res与SLPs质量比为1:32，磁搅拌2 h。在此条件下，SLPs-Res NPs的平均粒径为230.4±7.8 nm， Res包封率为（66.69±0.10）%。氢键和疏水力参与了纳米颗粒的形成。SLP-Res NPs表现出增强的光热稳定性和理想的缓释性能。对体外α-葡萄糖苷酶活性有明显抑制作用，对小鼠糖耐量试验有明显降糖作用。本研究提出了一种使用自组装slp递送Res的方法，证明了它们作为包封疏水生物活性分子的有效功能载体的潜力。

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引用次数: 0

Stereoselective synthesis of LacdiNAc N-glycan based on remote neighboring group participation 基于远邻基参与的LacdiNAc n -聚糖立体选择性合成。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-10-20 DOI: 10.1016/j.carres.2025.109709

Kenta Iino , Nozomi Ishii , Kanata Yoshida , Hideki Ishida , Yuji Matsuzaki , Ichiro Matsuo

LacdiNAc (GalNAcβ1-4GlcNAc) is a distinctive epitope found at the non-reducing termini of both N- and O-glycans. In recent years, the physiological functions of LacdiNAc have attracted increasing attention. Consequently, there is a significant demand for pure glycans for use in biochemical experiments. In this study, a concise and practical synthetic approach was developed for biantennary complex-type nonasaccharide 1, which contains LacdiNAc structures at the non-reducing end. Specifically, nonasaccharide 2 was initially constructed in a stereoselective manner via the condensation of trisaccharide donor 3 with trisaccharide acceptor 4, which bears hydroxy groups at the 3- and 6-positions of the β-mannose residue. Notably, this was achieved via the remote neighboring group participation of a pivaloyl group. Subsequent conversion of the N-phthalimido group into an acetamido group, followed by global deprotection, furnished the target compound, 1. The developed synthetic route represents a valuable tool for future investigations into LacdiNAc-modified N-glycans.

LacdiNAc （GalNAcβ1-4GlcNAc）是一个独特的表位，位于N-和o -聚糖的非还原末端。近年来，LacdiNAc的生理功能越来越受到人们的关注。因此，对用于生化实验的纯聚糖有很大的需求。本研究开发了一种简洁实用的双天线络合型非糖精1的合成方法，该非还原端含有LacdiNAc结构。具体来说，非糖2最初是通过三糖给体3和三糖受体4的缩合以立体选择性的方式构建的，三糖受体4在β-甘露糖残基的3位和6位上具有羟基。值得注意的是，这是通过远邻基参与的一个戊酰基实现的。随后n -邻苯二胺基转化为乙酰氨基基，然后进行全局脱保护，得到目标化合物，1。开发的合成路线为今后研究lacdinac修饰的n -聚糖提供了有价值的工具。

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引用次数: 0