Carbohydrate Research最新文献_第4页

Efficient conversion of biomass-derived xylose into furfural over boron phosphate solid acid in LiBr⋅3H2O/DCM biphasic system 在LiBr⋅3H2O/DCM双相体系中，磷酸硼固体酸催化生物质木糖高效转化为糠醛

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2026-01-07 DOI: 10.1016/j.carres.2026.109812

Wang Zhao , Jiansong Chen , Haishun Du , Zhiqiang Pang , Weiqi Wei , Xuejun Pan

Furfural is a pivotal intermediate in the biomass conversion. Therefore, the production of furfural from xylose or xylose-rich biomass has received extensive attention. Herein, a series of boron phosphate solid acid catalysts (BPs) were synthesized by self-assembly and high-temperature calcination, and used for the production of furfural from xylose or xylose-rich biomass. The results showed that the synthesized BPs was spherical nanoparticles and containing both Lewis acid and Brønsted acid sites, which was excellent for xylose conversion to furfural. The maximum furfural yield of this work was 82.8 % and achieved through 95.7 % xylose conversion by using 20 mg BP_1.25 at a reaction temperature of 130 °C for 120 min with a LiBr⋅3H₂O/dichloromethane (DCM) biphasic system. Noteworthy, the recycling experiments indicated that both the BP_1.25 catalyst and the LiBr⋅3H₂O can be reused at least four times with a furfural yield remaining ≥61.7 %. Last but not least, the prepared BP_1.25 catalyst also displayed high efficiency for xylose-rich natural biomass such as corncob and bagasse conversion into furfural. The work not only developed a new system for easy conversion of xylose into furfural, but also realized the high yield production of furfural from natural biomass without components separation.

糠醛是生物质转化的关键中间体。因此，利用木糖或富含木糖的生物质生产糠醛受到了广泛的关注。本文通过自组装和高温煅烧合成了一系列磷酸硼固体酸催化剂（BPs），用于木糖或富木糖生物质生产糠醛。结果表明，合成的bp为球形纳米颗粒，同时含有Lewis酸和Brønsted酸位点，有利于木糖转化为糠醛。在LiBr·3H2O/二氯甲烷（DCM）双相体系中，以20 mg BP1.25为原料，反应温度130℃，反应时间120 min，木糖转化率95.7%，糠醛收率最高可达82.8%。值得注意的是，回收实验表明，BP1.25催化剂和LiBr·3H2O均可重复使用至少4次，糠醛收率≥61.7%。最后，制备的BP1.25催化剂对玉米芯、甘蔗渣等富含木糖的天然生物质转化为糠醛也表现出较高的效率。本研究不仅开发了木糖易转化为糠醛的新体系，而且实现了天然生物质无需组分分离即可高产出糠醛。

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引用次数: 0

Enhanced antibacterial performance of chitosan via quaternary ammonium modification 季铵改性增强壳聚糖的抗菌性能。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-31 DOI: 10.1016/j.carres.2025.109811

Peixuan Wu , Hao Zhuang , Yang Zhou , Gaobo Yu , Zhendong Zhao , Tawfik Khattab , Xianghui Wang , Yuanyuan Liu

Chitosan (CS) exhibits pH- and concentration-dependent antimicrobial activity, underscoring the need to develop CS-based materials with more stable and robust antibacterial performance. In this work, dimethyloctadecyl-(3-trimethoxysilylpropyl) ammonium (TSA) was chemically grafted onto the hydroxyl and amino groups of CS to synthesize TSA-CS derivatives. Two forms of microspheres—designated as GA-TSA-CS-12 and STPP-TSA-CS-12—were subsequently fabricated via emulsion cross-linking and ionic cross-linking methods, respectively. The resulting TSA-CS materials were thoroughly characterized by FT-IR, TGA, GPC, SEM, and XPS, confirming successful grafting, modified thermal properties, and altered molecular weight. Antibacterial evaluation revealed that TSA-CS-12 achieved high inhibition rates of 97.7% against E. coli and 98.7% against S. aureus, while the corresponding microsphere formulations maintained up to 93% antibacterial activity. These findings demonstrate that TSA-functionalized chitosan materials exhibit enhanced and more stable antibacterial performance, showing promising potential for biomedical applications.

壳聚糖（CS）具有pH和浓度依赖性的抗菌活性，因此需要开发具有更稳定和强大抗菌性能的CS基材料。本研究将二甲基十八烷基-（3-三甲氧基硅丙基）铵（TSA）化学接枝到CS的羟基和氨基上，合成了TSA-CS衍生物。随后分别通过乳液交联和离子交联制备了两种形式的微球GA-TSA-CS-12和stpp - tsa - cs -12。通过FT-IR， TGA， GPC， SEM和XPS对TSA-CS材料进行了彻底的表征，证实了成功的接枝，改变了热性能，改变了分子量。抑菌评价表明，TSA-CS-12对大肠杆菌和金黄色葡萄球菌的抑菌率分别为97.7%和98.7%，其微球制剂的抑菌活性最高可达93%。这些研究结果表明，tsa功能化壳聚糖材料具有更强和更稳定的抗菌性能，具有良好的生物医学应用前景。

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引用次数: 0

Structural diversity of cyclodextrin metal-organic frameworks enables their diverse applications 环糊精金属-有机框架结构的多样性使其具有广泛的应用前景。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-30 DOI: 10.1016/j.carres.2025.109810

Edgar Clyde R. Lopez

Cyclodextrin-based metal-organic frameworks (CD-MOFs) constitute a distinctive family of porous solids that combine the modularity of coordination networks with the host-guest chemistry of renewable cyclic oligosaccharides. This review consolidates advances in the synthesis, structural diversity, and functional performance of CD-MOFs derived from α-, β-, and γ-cyclodextrins. Comparative structural analysis shows that framework topology and pore accessibility arise from coupled effects of cyclodextrin cavity size, metal coordination geometry, and synthetic route. Alkali-metal-linked frameworks generally form hydrophilic and biocompatible networks favorable for biomedical and environmental uses, while transition-metal incorporation improves mechanical strength and catalytic activity. Assessment of reported applications in gas storage, water purification, catalysis, and drug delivery demonstrates that performance trends correlate more strongly with accessible pore volume and chemical functionality than with crystallographic complexity. Constraints related to scale-up and hydrolytic stability continue to hinder practical adoption. This review advances transferable design principles that emphasize pore continuity, chemical resilience, and application-relevant metrics rather than structural intricacy. Progress in this field will depend on systematic benchmarking against established sorbents and the development of structure-property correlations grounded in realistic operating environments.

基于环糊精的金属有机框架（CD-MOFs）构成了一个独特的多孔固体家族，它结合了配位网络的模块化和可再生环状低聚糖的主客体化学。本文综述了由α-、β-和γ-环糊精衍生的CD-MOFs的合成、结构多样性和功能性能方面的研究进展。结构对比分析表明，环糊精空腔尺寸、金属配位几何形状和合成路线是框架拓扑结构和孔可达性的耦合效应。碱金属连接的框架通常形成有利于生物医学和环境用途的亲水性和生物相容性网络，而过渡金属的加入提高了机械强度和催化活性。在气体储存、水净化、催化和药物输送方面的应用报告表明，性能趋势与可达孔隙体积和化学功能的相关性比与晶体复杂性的相关性更强。与放大和水解稳定性相关的限制仍然阻碍着实际应用。这篇综述提出了可转移的设计原则，强调孔隙连续性、化学弹性和应用相关指标，而不是结构复杂性。该领域的进展将取决于对已建立的吸附剂的系统基准测试以及在实际操作环境中建立的结构-性能相关性的发展。

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引用次数: 0

Synthesis of Carbohydrate Derivatives in multicomponent reactions: Approaches and applications 多组分反应中碳水化合物衍生物的合成：方法和应用

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-29 DOI: 10.1016/j.carres.2025.109809

Isadora Maria Gouveia Andrade , Edson de Oliveira Lima Filho , Mariana de Oliveira Leal Desmarais, Caio Luiz Festas Valadão, Luana da Silva Magalhães Forezi, Fernando de Carvalho da Silva

This review presents a comprehensive overview of the principal strategies in the synthesis of carbohydrate derivatives through multicomponent reactions (MCRs), highlighting the significant potential of these methodologies in medicinal chemistry and the development of biocompatible materials. The inherent efficiency, atom economy, and modularity of MCRs make them particularly well-suited for assembling the structurally complex architectures characteristic of carbohydrate. These reactions facilitate the rapid generation of molecular diversity, enabling the design and synthesis of bioactive compounds with promising pharmacological profiles. In particular, the combination of carbohydrate scaffolds with diverse functional motifs through MCRs has opened new avenues for the discovery of novel therapeutic agents, including enzyme inhibitors, antimicrobial agents, and vaccine adjuvants. The review also addresses key stereochemical challenges associated with glycosylation and the formation of anomerically pure products, which remain central concerns in carbohydrate chemistry. Several representative examples from recent literature are discussed, demonstrating how MCR-based strategies have been successfully applied to the synthesis pseudo-glycopeptides, pseudo-glycolipids, and other carbohydrate derivatives of particular structures. These studies underscore the relevance of MCRs not only as a synthetic tool but also as a platform for innovation in drug development. Overall, the integration of MCRs into carbohydrate synthesis represents a powerful and versatile approach that aligns with current demands for more sustainable, efficient, and high-throughput methodologies in modern organic synthesis.

本文综述了通过多组分反应（mcr）合成碳水化合物衍生物的主要策略，并强调了这些方法在药物化学和生物相容性材料开发中的重要潜力。mcr固有的效率、原子经济性和模块化使它们特别适合于组装碳水化合物结构复杂的结构特征。这些反应促进了分子多样性的快速生成，使设计和合成具有药理前景的生物活性化合物成为可能。特别是，通过mcr将碳水化合物支架与多种功能基序结合，为发现新的治疗剂开辟了新的途径，包括酶抑制剂、抗菌剂和疫苗佐剂。综述还讨论了与糖基化和异构纯产物形成相关的关键立体化学挑战，这仍然是碳水化合物化学的核心问题。讨论了最近文献中的几个代表性例子，展示了基于mcr的策略如何成功地应用于合成伪糖肽、伪糖脂和其他特定结构的碳水化合物衍生物。这些研究强调了mcr不仅作为一种合成工具，而且作为药物开发创新平台的相关性。总的来说，将mcr整合到碳水化合物合成中代表了一种强大而通用的方法，符合现代有机合成中对更可持续、高效和高通量方法的当前需求。

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引用次数: 0

Towards recycling of ionic liquids in fiber spinning process –Carbohydrate transformation products in aqueous ionic liquid solutions studied by liquid chromatography/quadrupole time-of-flight mass spectrometry 纤维纺丝过程中离子液体的回收利用——液相色谱/四极杆飞行时间质谱法研究离子液体水溶液中碳水化合物转化产物

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-29 DOI: 10.1016/j.carres.2025.109808

Leena Pitkänen , Monika Tuominen , Behnaz Asadzadeh , Petri Uusi-Kyyny , Kalle Kaipanen , Petri Kilpeläinen , Michael Hummel , Herbert Sixta

New technologies for the production of man-made cellulose fibers are being developed to produce fibers sustainably for various textile applications. The Ioncell® process uses an ionic liquid in which cellulose is dissolved to form a spinning solution. This spinning solution is spun into an aqueous coagulation bath using dry-jet wet spinning technology to produce Ioncell fibres. In order to develop a sustainable and economically viable process, the ionic liquid must be efficiently recycled in the process. Organic compounds resulting from degradation reactions of the cellulosic materials used for fiber production might accumulate in the ionic liquid over time and reduce its dissolution power. This study aimed to tentatively identify the main carbohydrate transformation products from aqueous ionic liquid solution. In addition to the actual coagulation bath sample, carbohydrate transformation reactions were studied using model samples. The main monomeric carbohydrate constituents of a hardwood pulp, glucose and xylose, were mixed with an ionic liquid and water and heated to 90 °C for 8 h to accelerate the transformation reactions. Most of the original monosaccharides were converted into other compounds, so that after the heat treatment only 11 wt% of the glucose and 1.1 wt% of the xylose remained. The liquid chromatography/time-of-flight mass spectrometry analyses revealed that both the spin bath sample and model samples contained mainly hydroxycarboxylic acids and carboxylic acids. The superbase of ionic liquid catalyzed the alkaline transformation reactions of carbohydrates.

生产人造纤维素纤维的新技术正在开发中，以可持续地生产各种纺织用途的纤维。Ioncell®工艺使用溶解纤维素的离子液体形成纺丝溶液。使用干喷湿纺丝技术，将这种纺丝溶液纺成一个水凝浴，以生产离子细胞纤维。为了开发一种可持续且经济可行的工艺，必须在此过程中有效地回收离子液体。用于纤维生产的纤维素材料的降解反应产生的有机化合物可能随着时间的推移积聚在离子液体中并降低其溶解能力。本研究旨在初步确定离子液体水溶液中碳水化合物转化的主要产物。除了实际混凝液样品外，还使用模型样品研究了碳水化合物转化反应。将硬木纸浆的主要单体碳水化合物葡萄糖和木糖与离子液体和水混合，在90℃下加热8 h以加速转化反应。大多数原始单糖被转化为其他化合物，因此，经过热处理后，只有11%的葡萄糖和1.1 wt%的木糖保留下来。液相色谱/飞行时间质谱分析表明，旋转浴样品和模型样品主要含有羟基羧酸和羧酸。离子液体的超碱催化了碳水化合物的碱性转化反应。

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引用次数: 0

Selenocyanate-functionalized chitosan derivatives: Preparation, characterization, and evaluation of antioxidant activity 硒氰酸酯功能化壳聚糖衍生物：制备、表征和抗氧化活性评价

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-22 DOI: 10.1016/j.carres.2025.109807

Linqing Wang , Xiangru Han , Bing Ma , Yuting Yuan , Lijie Wei , Jingjing Zhang , Yingqi Mi , Zhanyong Guo , Fang Dong

This work describes the preparation of chitosan derivatives grafted with selenocyanate functional groups through a nucleophilic substitution reaction, with the objective of improving both their water solubility and antioxidant performance. Organic selenocyanate compounds were generated by reacting nicotinic acid and isonicotinic acid derivatives with 3-selenocyanatopropylamine, which were subsequently grafted onto chitosan molecules through nucleophilic substitution. The structures of the derivatives and their selenium content were characterized using Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (¹H NMR), and inductively coupled plasma mass spectrometry (ICP-MS). In vitro evaluation confirmed the superior antioxidant activity of the synthesized derivatives. Specifically, they exhibited excellent DPPH radical scavenging rates of over 70 % at 0.4 mg/mL, indicating a significant enhancement compared to native chitosan. Cytotoxicity assays (MTT method) indicated that most derivatives possessed good biocompatibility. In particular, derivatives 3a, 3b, 3f, and 4c showed cell viability rates above 80 % within the tested concentration range, suggesting their potential as novel antioxidants and selenium supplements. This study offers a novel approach to broaden the utilization of chitosan in food, nutraceutical, and pharmaceutical applications.

本文通过亲核取代反应制备了硒氰酸酯官能团接枝的壳聚糖衍生物，以提高其水溶性和抗氧化性能。由烟酸和异烟酸衍生物与3-硒氰酸异丙胺反应生成有机硒氰酸酯化合物，并通过亲核取代接枝到壳聚糖分子上。采用傅里叶变换红外光谱（FTIR）、质子核磁共振（1H NMR）和电感耦合等离子体质谱（ICP-MS）对衍生物的结构和硒含量进行了表征。体外评价证实了所合成的衍生物具有良好的抗氧化活性。具体来说，它们在0.4 mg/mL时表现出超过70%的DPPH自由基清除率，与天然壳聚糖相比显着增强。细胞毒性试验（MTT法）表明，大多数衍生物具有良好的生物相容性。特别是，衍生物3a、3b、3f和4c在测试浓度范围内的细胞存活率超过80%，表明它们具有作为新型抗氧化剂和硒补充剂的潜力。本研究为壳聚糖在食品、保健品、医药等领域的广泛应用提供了一条新的途径。

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引用次数: 0

Water-soluble conjugates with antitumor activity from starch, phenolic antioxidant and calcium salt 淀粉、酚类抗氧化剂和钙盐的水溶性抗肿瘤结合物。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-22 DOI: 10.1016/j.carres.2025.109806

V.V. Spiridonov , M.A. Torlopov , I. Yu Chukicheva , A.V. Kutchin , D.V. Pozdyshev , V.I. Muronetz , A.A. Yaroslavov

Four samples, synthesized from electroneutral and positive starches with covalently attached phenolic antioxidant Dibornol, were dissolved in water and conjugated with biogenic Ca²⁺ and Mg²⁺-cations. The positive conjugate with involved Ca²⁺-cations showed a pronounced activity towards tumor cells MCF7. The obtained results show a way for design and development of antitumor formulations.

四种样品分别由电中性和正电性淀粉与共价附着的酚类抗氧化剂二冰毒合成，溶解于水中，并与生物源性Ca2+和Mg2+阳离子偶联。与相关Ca2+阳离子的正偶联对肿瘤细胞MCF7有明显的活性。所得结果为抗肿瘤制剂的设计和开发提供了一条途径。

引用次数: 0

A short synthesis of p-nitrophenyl α-d-xylofuranoside and β-d-arabinofuranoside employing a Mitsunobu glycosylation 用Mitsunobu糖基化法合成了对硝基苯α-d-木呋喃苷和β-d-阿拉伯呋喃苷。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-19 DOI: 10.1016/j.carres.2025.109802

Zongjia Chen, Adam W.E. Stewart, Spencer J. Williams

Chromogenic p-nitrophenyl glycosides are widely used substrates for glycosidase assays, yet analogous derivatives of rare pentofuranoses remain largely unavailable. We report concise five-step syntheses of p-nitrophenyl β-d-arabinofuranoside and p-nitrophenyl α-d-xylofuranoside using a Mitsunobu glycosylation between the corresponding sugar hemiacetals and p-nitrophenol. The use of benzyl-protected donors simplifies preparation of the furanosyl donors, and mild oxidative debenzylation (KBrO₃/Na₂S₂O₄) preserves the nitro group. Both glycosides were obtained in good overall yield, and their structures, including anomeric configurations, were confirmed by single-crystal X-ray diffraction. This route furnishes practical access to these useful chromogenic furanosides, which will facilitate biochemical and mechanistic studies of α-d-xylofuranosidases, β-d-arabinofuranosidases, and related enzymes of pentofuranoside metabolism.

显色对硝基苯基糖苷是糖苷酶测定中广泛使用的底物，但罕见的戊呋喃糖的类似衍生物仍然很大程度上不可用。我们报道了用相应的糖半缩醛和对硝基苯酚之间的Mitsunobu糖基化，简明地合成了对硝基苯基β-d-阿拉伯呋喃苷和对硝基苯基α-d-木呋喃苷的五步法。使用受苄基保护的给体简化了呋喃烷基给体的制备，并且轻度氧化脱苄基（KBrO3/Na2S2O4）保留了硝基。这两种糖苷的总产率都很高，其结构（包括异构体）通过单晶x射线衍射得到了证实。这条途径提供了这些有用的显色呋喃苷的实际途径，这将有助于α-d-木糖呋喃苷酶、β-d-阿拉伯糖呋喃苷酶和戊呋喃苷代谢相关酶的生化和机制研究。

引用次数: 0

Structural analysis, pharmacological mechanism, pharmaceutics, and comparison of differences between raw and processed product of polysaccharide from Rehmannia glutinosa: A review 地黄多糖的结构分析、药理机制、药理作用及生制品与加工制品的差异比较

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-15 DOI: 10.1016/j.carres.2025.109794

Chunni Zhao , Lianyuan Tang , Xin Guo , Huan Liang , Ding Tian , Mei Zhang

Rehmannia glutinosa (RG) is a widely used medicinal plant, which was included in China's Medicine Food Homology list in 2024. Rehmannia glutinosa polysaccharide (RGP) is one of the primary and most representative pharmacologically active macromolecules of RG, which has been shown in numerous in vitro and in vivo pharmacological studies to exhibit a variety of pharmacological activities, including immunological regulation, intestinal protection, anti-osteoporosis, anti-anemia, liver and kidney protection, anti-hyperthyroidism, reproductive protection, treatment of cataract, anti-anxiety, anti-aging, and anti-mutagenic properties. To support their therapeutic potential and health functions, this review aims to thoroughly and methodically sort the information on the structural characteristics, biological activities and potential mechanisms of action, pharmaceutics research, and differences between raw and processed RGP. At the same time, it offers fresh, insightful information for further RGP research in the areas of medicinal agents and functional foods.

地黄（Rehmannia glutinosa， RG）是一种应用广泛的药用植物，于2024年被列入中国药食同源名录。地黄多糖（RGP）是地黄主要的、最具代表性的药理活性大分子之一，大量体外和体内药理研究表明，地黄多糖具有多种药理活性，包括免疫调节、肠道保护、抗骨质疏松、抗贫血、肝肾保护、抗甲状腺功能亢进、生殖保护、治疗白内障、抗焦虑、抗衰老、以及抗诱变特性。为了支持其治疗潜力和保健功能，本文对其结构特征、生物活性和潜在作用机制、药理研究以及生加工和加工的差异进行了全面、系统的梳理。同时，它为进一步在医药制剂和功能食品领域的RGP研究提供了新鲜的、有见地的信息。

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引用次数: 0

Biochemical and functional analysis of a thermostable, xylose-tolerant glycoside hydrolase 43 β-xylosidase from Thermothelomyces thermophilus 嗜热热菌耐热耐木糖糖苷水解酶43 β-木糖糖苷酶的生化和功能分析。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-15 DOI: 10.1016/j.carres.2025.109789

Ravi Kumar , Shilpa Sharma , Tulasi Satyanarayana

The catalytic degradation of hemicellulose, particularly xylan, is critical for converting lignocellulosic biomass into economically valuable monosaccharides like xylose. β-xylosidases play a key role in this process by enabling efficient breakdown of xylan. Herein, a novel thermostable, xylose tolerant β-xylosidase from Thermothelomyces thermophilus (TtGH43) was codon-optimized, cloned and heterologously expressed in Escherichia coli BL21 (DE3), for the first time. Sequence analysis classified the enzyme as glycoside hydrolase 43 (GH43), sharing homology with fungal and bacterial GH43 β-xylosidases. Structural modelling confirmed the presence of conserved catalytic residues (glutamic and aspartic acid) critical for substrate binding and hydrolysis. Biochemical characterization revealed optimal activity of recombinant enzyme at 60 °C and pH 6.0, with robust stability under alkaline conditions. TtGH43 exhibited β-xylosidase activity towards synthetic substrates (e.g., p-nitrophenyl-β-d-xylopyranoside) as well as ability to degrade natural xylo-oligosaccharides. The enzyme demonstrated xylose tolerance up to 750 mM and retained activity in the presence of various metal ions and chemical additives. Additionally, TtGH43 maintained functionality under high-salt conditions, a rare-trait among fungal β-xylosidases. Overall, this study highlights TtGH43 as a promising enzyme candidate for industrial processes that demand alkali-tolerant and thermostable enzymes for efficient lignocellulosic biomass degradation.

催化降解半纤维素，特别是木聚糖，对于将木质纤维素生物质转化为具有经济价值的单糖（如木糖）至关重要。β-木糖苷酶在这一过程中发挥关键作用，使木聚糖的有效分解。本文首次在大肠杆菌BL21 （DE3）中进行密码子优化、克隆并异源表达了一种新的耐热、耐木糖的β-木糖苷酶（TtGH43）。序列分析表明该酶为糖苷水解酶43 (GH43)，与真菌和细菌GH43 β-木糖苷酶具有同源性。结构模型证实了保守的催化残基（谷氨酸和天冬氨酸）的存在，这对底物的结合和水解至关重要。生化表征表明，重组酶在60℃、pH 6.0条件下活性最佳，在碱性条件下具有较强的稳定性。TtGH43对合成底物（如对硝基苯-β-d-木吡喃苷）具有β-木糖苷酶活性，并具有降解天然低聚木糖的能力。该酶对木糖的耐受性高达750 mM，在各种金属离子和化学添加剂的存在下仍保持活性。此外，TtGH43在高盐条件下保持功能，这是真菌β-木糖苷酶中罕见的特性。总的来说，这项研究突出了TtGH43作为一种有前途的候选酶，用于工业过程，这些过程需要耐碱和耐热酶来进行有效的木质纤维素生物质降解。

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引用次数: 0