Carbohydrate Research最新文献_第2页

Towards recycling of ionic liquids in fiber spinning process –Carbohydrate transformation products in aqueous ionic liquid solutions studied by liquid chromatography/quadrupole time-of-flight mass spectrometry 纤维纺丝过程中离子液体的回收利用——液相色谱/四极杆飞行时间质谱法研究离子液体水溶液中碳水化合物转化产物

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-29 DOI: 10.1016/j.carres.2025.109808

Leena Pitkänen , Monika Tuominen , Behnaz Asadzadeh , Petri Uusi-Kyyny , Kalle Kaipanen , Petri Kilpeläinen , Michael Hummel , Herbert Sixta

New technologies for the production of man-made cellulose fibers are being developed to produce fibers sustainably for various textile applications. The Ioncell® process uses an ionic liquid in which cellulose is dissolved to form a spinning solution. This spinning solution is spun into an aqueous coagulation bath using dry-jet wet spinning technology to produce Ioncell fibres. In order to develop a sustainable and economically viable process, the ionic liquid must be efficiently recycled in the process. Organic compounds resulting from degradation reactions of the cellulosic materials used for fiber production might accumulate in the ionic liquid over time and reduce its dissolution power. This study aimed to tentatively identify the main carbohydrate transformation products from aqueous ionic liquid solution. In addition to the actual coagulation bath sample, carbohydrate transformation reactions were studied using model samples. The main monomeric carbohydrate constituents of a hardwood pulp, glucose and xylose, were mixed with an ionic liquid and water and heated to 90 °C for 8 h to accelerate the transformation reactions. Most of the original monosaccharides were converted into other compounds, so that after the heat treatment only 11 wt% of the glucose and 1.1 wt% of the xylose remained. The liquid chromatography/time-of-flight mass spectrometry analyses revealed that both the spin bath sample and model samples contained mainly hydroxycarboxylic acids and carboxylic acids. The superbase of ionic liquid catalyzed the alkaline transformation reactions of carbohydrates.

生产人造纤维素纤维的新技术正在开发中，以可持续地生产各种纺织用途的纤维。Ioncell®工艺使用溶解纤维素的离子液体形成纺丝溶液。使用干喷湿纺丝技术，将这种纺丝溶液纺成一个水凝浴，以生产离子细胞纤维。为了开发一种可持续且经济可行的工艺，必须在此过程中有效地回收离子液体。用于纤维生产的纤维素材料的降解反应产生的有机化合物可能随着时间的推移积聚在离子液体中并降低其溶解能力。本研究旨在初步确定离子液体水溶液中碳水化合物转化的主要产物。除了实际混凝液样品外，还使用模型样品研究了碳水化合物转化反应。将硬木纸浆的主要单体碳水化合物葡萄糖和木糖与离子液体和水混合，在90℃下加热8 h以加速转化反应。大多数原始单糖被转化为其他化合物，因此，经过热处理后，只有11%的葡萄糖和1.1 wt%的木糖保留下来。液相色谱/飞行时间质谱分析表明，旋转浴样品和模型样品主要含有羟基羧酸和羧酸。离子液体的超碱催化了碳水化合物的碱性转化反应。

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引用次数: 0

Selenocyanate-functionalized chitosan derivatives: Preparation, characterization, and evaluation of antioxidant activity 硒氰酸酯功能化壳聚糖衍生物：制备、表征和抗氧化活性评价

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-22 DOI: 10.1016/j.carres.2025.109807

Linqing Wang , Xiangru Han , Bing Ma , Yuting Yuan , Lijie Wei , Jingjing Zhang , Yingqi Mi , Zhanyong Guo , Fang Dong

This work describes the preparation of chitosan derivatives grafted with selenocyanate functional groups through a nucleophilic substitution reaction, with the objective of improving both their water solubility and antioxidant performance. Organic selenocyanate compounds were generated by reacting nicotinic acid and isonicotinic acid derivatives with 3-selenocyanatopropylamine, which were subsequently grafted onto chitosan molecules through nucleophilic substitution. The structures of the derivatives and their selenium content were characterized using Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (¹H NMR), and inductively coupled plasma mass spectrometry (ICP-MS). In vitro evaluation confirmed the superior antioxidant activity of the synthesized derivatives. Specifically, they exhibited excellent DPPH radical scavenging rates of over 70 % at 0.4 mg/mL, indicating a significant enhancement compared to native chitosan. Cytotoxicity assays (MTT method) indicated that most derivatives possessed good biocompatibility. In particular, derivatives 3a, 3b, 3f, and 4c showed cell viability rates above 80 % within the tested concentration range, suggesting their potential as novel antioxidants and selenium supplements. This study offers a novel approach to broaden the utilization of chitosan in food, nutraceutical, and pharmaceutical applications.

本文通过亲核取代反应制备了硒氰酸酯官能团接枝的壳聚糖衍生物，以提高其水溶性和抗氧化性能。由烟酸和异烟酸衍生物与3-硒氰酸异丙胺反应生成有机硒氰酸酯化合物，并通过亲核取代接枝到壳聚糖分子上。采用傅里叶变换红外光谱（FTIR）、质子核磁共振（1H NMR）和电感耦合等离子体质谱（ICP-MS）对衍生物的结构和硒含量进行了表征。体外评价证实了所合成的衍生物具有良好的抗氧化活性。具体来说，它们在0.4 mg/mL时表现出超过70%的DPPH自由基清除率，与天然壳聚糖相比显着增强。细胞毒性试验（MTT法）表明，大多数衍生物具有良好的生物相容性。特别是，衍生物3a、3b、3f和4c在测试浓度范围内的细胞存活率超过80%，表明它们具有作为新型抗氧化剂和硒补充剂的潜力。本研究为壳聚糖在食品、保健品、医药等领域的广泛应用提供了一条新的途径。

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引用次数: 0

Water-soluble conjugates with antitumor activity from starch, phenolic antioxidant and calcium salt 淀粉、酚类抗氧化剂和钙盐的水溶性抗肿瘤结合物。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-22 DOI: 10.1016/j.carres.2025.109806

V.V. Spiridonov , M.A. Torlopov , I. Yu Chukicheva , A.V. Kutchin , D.V. Pozdyshev , V.I. Muronetz , A.A. Yaroslavov

Four samples, synthesized from electroneutral and positive starches with covalently attached phenolic antioxidant Dibornol, were dissolved in water and conjugated with biogenic Ca²⁺ and Mg²⁺-cations. The positive conjugate with involved Ca²⁺-cations showed a pronounced activity towards tumor cells MCF7. The obtained results show a way for design and development of antitumor formulations.

四种样品分别由电中性和正电性淀粉与共价附着的酚类抗氧化剂二冰毒合成，溶解于水中，并与生物源性Ca2+和Mg2+阳离子偶联。与相关Ca2+阳离子的正偶联对肿瘤细胞MCF7有明显的活性。所得结果为抗肿瘤制剂的设计和开发提供了一条途径。

引用次数: 0

A short synthesis of p-nitrophenyl α-d-xylofuranoside and β-d-arabinofuranoside employing a Mitsunobu glycosylation 用Mitsunobu糖基化法合成了对硝基苯α-d-木呋喃苷和β-d-阿拉伯呋喃苷。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-19 DOI: 10.1016/j.carres.2025.109802

Zongjia Chen, Adam W.E. Stewart, Spencer J. Williams

Chromogenic p-nitrophenyl glycosides are widely used substrates for glycosidase assays, yet analogous derivatives of rare pentofuranoses remain largely unavailable. We report concise five-step syntheses of p-nitrophenyl β-d-arabinofuranoside and p-nitrophenyl α-d-xylofuranoside using a Mitsunobu glycosylation between the corresponding sugar hemiacetals and p-nitrophenol. The use of benzyl-protected donors simplifies preparation of the furanosyl donors, and mild oxidative debenzylation (KBrO₃/Na₂S₂O₄) preserves the nitro group. Both glycosides were obtained in good overall yield, and their structures, including anomeric configurations, were confirmed by single-crystal X-ray diffraction. This route furnishes practical access to these useful chromogenic furanosides, which will facilitate biochemical and mechanistic studies of α-d-xylofuranosidases, β-d-arabinofuranosidases, and related enzymes of pentofuranoside metabolism.

显色对硝基苯基糖苷是糖苷酶测定中广泛使用的底物，但罕见的戊呋喃糖的类似衍生物仍然很大程度上不可用。我们报道了用相应的糖半缩醛和对硝基苯酚之间的Mitsunobu糖基化，简明地合成了对硝基苯基β-d-阿拉伯呋喃苷和对硝基苯基α-d-木呋喃苷的五步法。使用受苄基保护的给体简化了呋喃烷基给体的制备，并且轻度氧化脱苄基（KBrO3/Na2S2O4）保留了硝基。这两种糖苷的总产率都很高，其结构（包括异构体）通过单晶x射线衍射得到了证实。这条途径提供了这些有用的显色呋喃苷的实际途径，这将有助于α-d-木糖呋喃苷酶、β-d-阿拉伯糖呋喃苷酶和戊呋喃苷代谢相关酶的生化和机制研究。

引用次数: 0

Structural analysis, pharmacological mechanism, pharmaceutics, and comparison of differences between raw and processed product of polysaccharide from Rehmannia glutinosa: A review 地黄多糖的结构分析、药理机制、药理作用及生制品与加工制品的差异比较

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-15 DOI: 10.1016/j.carres.2025.109794

Chunni Zhao , Lianyuan Tang , Xin Guo , Huan Liang , Ding Tian , Mei Zhang

Rehmannia glutinosa (RG) is a widely used medicinal plant, which was included in China's Medicine Food Homology list in 2024. Rehmannia glutinosa polysaccharide (RGP) is one of the primary and most representative pharmacologically active macromolecules of RG, which has been shown in numerous in vitro and in vivo pharmacological studies to exhibit a variety of pharmacological activities, including immunological regulation, intestinal protection, anti-osteoporosis, anti-anemia, liver and kidney protection, anti-hyperthyroidism, reproductive protection, treatment of cataract, anti-anxiety, anti-aging, and anti-mutagenic properties. To support their therapeutic potential and health functions, this review aims to thoroughly and methodically sort the information on the structural characteristics, biological activities and potential mechanisms of action, pharmaceutics research, and differences between raw and processed RGP. At the same time, it offers fresh, insightful information for further RGP research in the areas of medicinal agents and functional foods.

地黄（Rehmannia glutinosa， RG）是一种应用广泛的药用植物，于2024年被列入中国药食同源名录。地黄多糖（RGP）是地黄主要的、最具代表性的药理活性大分子之一，大量体外和体内药理研究表明，地黄多糖具有多种药理活性，包括免疫调节、肠道保护、抗骨质疏松、抗贫血、肝肾保护、抗甲状腺功能亢进、生殖保护、治疗白内障、抗焦虑、抗衰老、以及抗诱变特性。为了支持其治疗潜力和保健功能，本文对其结构特征、生物活性和潜在作用机制、药理研究以及生加工和加工的差异进行了全面、系统的梳理。同时，它为进一步在医药制剂和功能食品领域的RGP研究提供了新鲜的、有见地的信息。

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引用次数: 0

Biochemical and functional analysis of a thermostable, xylose-tolerant glycoside hydrolase 43 β-xylosidase from Thermothelomyces thermophilus 嗜热热菌耐热耐木糖糖苷水解酶43 β-木糖糖苷酶的生化和功能分析。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-15 DOI: 10.1016/j.carres.2025.109789

Ravi Kumar , Shilpa Sharma , Tulasi Satyanarayana

The catalytic degradation of hemicellulose, particularly xylan, is critical for converting lignocellulosic biomass into economically valuable monosaccharides like xylose. β-xylosidases play a key role in this process by enabling efficient breakdown of xylan. Herein, a novel thermostable, xylose tolerant β-xylosidase from Thermothelomyces thermophilus (TtGH43) was codon-optimized, cloned and heterologously expressed in Escherichia coli BL21 (DE3), for the first time. Sequence analysis classified the enzyme as glycoside hydrolase 43 (GH43), sharing homology with fungal and bacterial GH43 β-xylosidases. Structural modelling confirmed the presence of conserved catalytic residues (glutamic and aspartic acid) critical for substrate binding and hydrolysis. Biochemical characterization revealed optimal activity of recombinant enzyme at 60 °C and pH 6.0, with robust stability under alkaline conditions. TtGH43 exhibited β-xylosidase activity towards synthetic substrates (e.g., p-nitrophenyl-β-d-xylopyranoside) as well as ability to degrade natural xylo-oligosaccharides. The enzyme demonstrated xylose tolerance up to 750 mM and retained activity in the presence of various metal ions and chemical additives. Additionally, TtGH43 maintained functionality under high-salt conditions, a rare-trait among fungal β-xylosidases. Overall, this study highlights TtGH43 as a promising enzyme candidate for industrial processes that demand alkali-tolerant and thermostable enzymes for efficient lignocellulosic biomass degradation.

催化降解半纤维素，特别是木聚糖，对于将木质纤维素生物质转化为具有经济价值的单糖（如木糖）至关重要。β-木糖苷酶在这一过程中发挥关键作用，使木聚糖的有效分解。本文首次在大肠杆菌BL21 （DE3）中进行密码子优化、克隆并异源表达了一种新的耐热、耐木糖的β-木糖苷酶（TtGH43）。序列分析表明该酶为糖苷水解酶43 (GH43)，与真菌和细菌GH43 β-木糖苷酶具有同源性。结构模型证实了保守的催化残基（谷氨酸和天冬氨酸）的存在，这对底物的结合和水解至关重要。生化表征表明，重组酶在60℃、pH 6.0条件下活性最佳，在碱性条件下具有较强的稳定性。TtGH43对合成底物（如对硝基苯-β-d-木吡喃苷）具有β-木糖苷酶活性，并具有降解天然低聚木糖的能力。该酶对木糖的耐受性高达750 mM，在各种金属离子和化学添加剂的存在下仍保持活性。此外，TtGH43在高盐条件下保持功能，这是真菌β-木糖苷酶中罕见的特性。总的来说，这项研究突出了TtGH43作为一种有前途的候选酶，用于工业过程，这些过程需要耐碱和耐热酶来进行有效的木质纤维素生物质降解。

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引用次数: 0

Site-selective CE4 enzyme from Rhodothermus marinus and diversified LmbE-Like catalysis in Bacillus cereus marinus Rhodothermus的位点选择性CE4酶及其在蜡样芽孢杆菌中的多种lmbe样催化作用。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-13 DOI: 10.1016/j.carres.2025.109792

Jun Bian, Pai Zang, Ran Tang, Can Wei, Yaqing Wang, Li Liu, Josef Voglmeir

Chitooligosaccharides (COS) display diverse biological functions that depend strongly on their N-acetylation pattern, motivating enzymatic approaches that enable regioselective deacetylation. Here, we identified a CE4 deacetylase, RmCBDA2510 from Rhodothermus marinus that performs strict, site-selective, and chain-length-dependent mono-deacetylation of COS. Exoglycosidase probing together with MALDI-TOF/TOF MS/MS showed that RmCBDA2510 precisely generates AD (GlcNAc-GlcN), ADA (GlcNAc-GlcN-GlcNAc), and AADA (GlcNAc-GlcNAc-GlcN-GlcNAc) from chitobiose, chitotriose, and chitotetraose, respectively, revealing a distinctive internal subsite preference. The enzyme is thermophilic and alkaline-active, and site-directed mutagenesis identified Asp41 as essential for catalysis, establishing RmCBDA2510 as a highly selective biocatalyst for producing structurally uniform, partially deacetylated COS.

In contrast, the LmbE-like enzyme BCLmbE1534 from Bacillus cereus catalyzes both the deacetylation of N-acetylglucosamine glycosides and acylation/trans-acylation with short-chain fatty acids and biotin. This represents the first demonstration of trans-acylation within the LmbE family and expands its catalytic repertoire beyond simple deacetylation. Together, RmCBDA2510 and BCLmbE1534 provide complementary enzymatic platforms for tailoring COS structures and diversifying amino-sugar derivatives, thereby broadening the synthetic utility of carbohydrate-active deacetylases.

壳寡糖（COS）显示出多种生物学功能，这些功能强烈依赖于它们的n -乙酰化模式，从而激发酶的方法来实现区域选择性去乙酰化。在这里，我们从海Rhodothermus marinus中鉴定了一种CE4去乙酰化酶RmCBDA2510，该酶能对COS进行严格的、位点选择性的、链长依赖性的单去乙酰化。外糖苷酶检测结合MALDI-TOF/TOF质谱/质谱分析显示，RmCBDA2510分别从壳聚糖、壳三糖和壳四糖中精确生成AD （GlcNAc-GlcN）、ADA （GlcNAc-GlcN- glcnac）和AADA (GlcNAc-GlcNAc-GlcN-GlcNAc)，显示出独特的内部亚位偏好。该酶嗜热和碱活性，位点定向诱变鉴定出Asp41是催化的必要条件，建立了RmCBDA2510作为一种高度选择性的生物催化剂，用于生产结构均匀，部分去乙酰化的COS。相比之下，蜡样芽孢杆菌的lmbe样酶BCLmbE1534既能催化n -乙酰氨基葡萄糖苷的去乙酰化，也能催化短链脂肪酸和生物素的酰化/反酰化。这代表了LmbE家族中反式酰化的第一次演示，并扩展了其催化功能，超越了简单的去乙酰化。RmCBDA2510和BCLmbE1534共同为裁剪COS结构和多样化氨基糖衍生物提供了互补的酶促平台，从而扩大了碳水化合物活性脱乙酰酶的合成用途。

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引用次数: 0

Characterization and engineering of Drosophila melanogaster β1-3-galactosyltransferase for glycoengineering applications 黑胃果蝇β1-3-半乳糖基转移酶的表征与工程应用。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-12 DOI: 10.1016/j.carres.2025.109791

Linhan Wang , Jichao Wei , Fang Yuan , Yongheng Rong , Wanqian Du , Wenjing Shi , Xintan Wang , Mei Wang , Yankang Wang , Anran Liu , Junqiao Zhao , Yun Kong , Na Sun , Wenzhu Tang , Shengjun Wang

β1-3-Galactosyltransferase from Drosophila melanogaster (DmC1GalT1) plays a key role in O-glycan synthesis and holds considerable potential for glycoengineering applications. In this study, we achieved high-yield expression and purification of DmC1GalT1 in Escherichia coli, obtaining over 5 mg of protein per liter of culture. Similar with the enzyme expressed from Pichia pastoris, this enzyme exhibited strict donor specificity towards UDP-Gal and efficiently galactosylated human CD74-derived Tn-glycopeptides. Structural analysis identified key residues involved in substrate binding and catalysis. Site-directed mutagenesis of active-site residues yielded variants with altered activity and stability profiles. Notably, the N108G and Y325W mutants retained high activity towards UDP-Gal but lost the ability to utilize UDP-GalNH₂, while N108A mutants retained 61.77 % of its activity toward UDP-Gal but maintained similar activity levels towards UDP-GalNH₂, while gaining weak but detectable activity toward UDP-Glc. These findings highlight the critical roles of residues N108 and Y325 in donor recognition. Thermal stability predictions using ProStab indicated that certain mutations affected enzyme stability without compromising activity. This work establishes an efficient platform for the expression, characterization, and engineering of DmC1GalT1, facilitating the development of glycosyltransferase variants with tailored properties for synthetic glycobiology.

黑腹果蝇β1-3-半乳糖转移酶（DmC1GalT1）在o -聚糖合成中起着关键作用，在糖工程方面具有相当大的应用潜力。在本研究中，我们在大肠杆菌中实现了DmC1GalT1的高产表达和纯化，每升培养液中蛋白含量超过5 mg。与毕赤酵母中表达的酶类似，该酶对UDP-Gal具有严格的供体特异性，并能有效地半乳糖化人cd74衍生的n-糖肽。结构分析确定了参与底物结合和催化的关键残基。活性位点残基的定点诱变产生了具有改变的活性和稳定性的变异。值得注意的是，N108G和Y325W突变体对UDP-Gal保持了高活性，但失去了利用UDP-GalNH2的能力，而N108A突变体对UDP-Gal保持了61.77%的活性，但对UDP-GalNH2保持了相似的活性水平，而对UDP-Glc的活性较弱，但可检测到。这些发现突出了N108和Y325残基在供体识别中的关键作用。使用ProStab进行热稳定性预测表明，某些突变会影响酶的稳定性，但不会影响酶的活性。这项工作为DmC1GalT1的表达、表征和工程建立了一个有效的平台，促进了糖基转移酶变体的开发，这些变体具有适合合成糖生物学的特性。

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引用次数: 0

Uncovering the β-1,6-galactan-degrading machinery: Biochemical and functional insights into a processive exo-β-1,6-galactanase, RfGH30 from Ruminococcus flavefaciens 揭示β-1,6-半乳糖降解机制：来自黄瘤胃球菌的外源性-β-1,6-半乳糖酶RfGH30的生化和功能见解。

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-12 DOI: 10.1016/j.carres.2025.109793

Shreya Biswas, Gurleen Kaur Bagga, Parmeshwar Vitthal Gavande , Arun Goyal

The glycoside hydrolase family 30 (GH30) enzyme RfGH30, originating from the ruminal bacterium Ruminococcus flavefaciens, was biochemically characterized to elucidate its catalytic properties and substrate specificity. Although initially classified as endoxylanase, experimental analysis confirmed RfGH30 as processive exo-β-1,6-galactanase targeting galactan-rich polysaccharides. Zymogram analysis revealed a clear hydrolytic zone at ∼54 kDa on larchwood arabinogalactan (LWAG), confirming its specificity for β-1,6-glycosidic linkage containing galactose units. The enzyme exhibited optimum pH 5.5 and temperature 40 °C, retaining stability across pH 3.8–7.0 and temperature range, 4–70 °C. Among natural substrates, RfGH30 displayed highest specific activity, 8.1 U mg⁻¹ against LWAG followed by arabinan 4.6 U mg⁻¹ against sugarbeet arabinan. RfGH30 also hydrolysed synthetic substrates, pNPG and oNPG consistent with an exo-mode of catalysis. Kinetic analysis showed a V_max of 9.8 U mg⁻¹ and K_M of 1.45 mg mL⁻¹ against LWAG. Enzyme activity was enhanced by Ca²⁺ and Mg²⁺, but inhibited by Fe²⁺ and the chelating agent EGTA. RfGH30 hydrolysed LWAG product profile by TLC, HPLC and HRMS revealed the predominant release of β-1,6-galactobiose along with minor galactose, demonstrating strict cleavage of β-1,6-galactosyl linkages. RfGH30 showed strong processivity with LWAG, with the high processivity index of 21 and a low, 4.7 with sugarbeet arabinan. This study reports the first biochemical evidence of a GH30 processive exo-β-1,6-galactanase from R. flavefaciens, providing insights into its role in ruminal degradation of plant arabinogalactans and its potential applications in biomass valorization.

糖苷水解酶家族30 （GH30）酶RfGH30源自瘤胃细菌黄瘤球菌，对其进行了生化表征，以阐明其催化特性和底物特异性。虽然最初被归类为内木聚糖酶，但实验分析证实RfGH30是针对富含半乳糖多糖的显性外显子-β-1,6-半乳糖酶。酶谱分析显示，落叶松阿拉伯半乳糖（LWAG）在~ 54 kDa处有一个清晰的水解区，证实了它对含有半乳糖单元的β-1,6-糖苷连锁的特异性。该酶在pH为5.5、温度为40℃时表现出最佳稳定性，在pH为3.8 ~ 7.0、温度为4 ~ 70℃范围内均保持稳定。在天然底物中，RfGH30对LWAG的比活性最高，为8.1 U mg-1，其次是arabinan，对甜菜arabinan的比活性为4.6 U mg-1。RfGH30还水解合成底物pNPG和oNPG，符合外显催化模式。动力学分析表明，对LWAG的Vmax为9.8 U mg-1， KM为1.45 mg mL-1。Ca2+和Mg2+对酶活性有增强作用，Fe2+和EGTA对酶活性有抑制作用。通过TLC、HPLC和HRMS分析，RfGH30水解后的LWAG产物主要释放β-1,6-半乳糖糖和少量半乳糖，显示了β-1,6-半乳糖键的严格断裂。RfGH30对LWAG的加工性较强，加工性指数高，为21，对阿拉伯甜菜聚糖的加工性指数低，为4.7。本研究首次报道了黄芽霉GH30进程性外显-β-1,6-半乳糖酶的生化证据，为其在植物阿拉伯半乳糖瘤胃降解中的作用及其在生物质增值中的潜在应用提供了新的见解。

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引用次数: 0

Preparation of carboxymethyl potato starch using a new sodium carbonate pretreatment process and its microencapsulation application 碳酸钠预处理新工艺制备马铃薯羧甲基淀粉及其微胶囊化应用

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research

Pub Date : 2025-12-11 DOI: 10.1016/j.carres.2025.109790

Yong Chen, Cheng Tang, Ziqiang Zhao, Zhimin Liu, Jiajia Huang, Qijie Chen

Carboxymethyl starch (CMSt) is a modified starch with excellent performance. In this study, sodium carbonate pretreatment was used to promote the carboxymethylation reaction activity of starch, carboxymethyl starch with medium and low substitution degrees was successfully prepared, and the synthesis parameters of carboxymethyl starch were optimized using response surface methodology. The results showed that when the pretreatment concentration was 0.95 mol/L, the pretreatment time was 54 min, the molar ratio of alkali to starch was 1.3, and the molar ratio of acid to starch was 1, the carboxymethyl starch with a degree of substitution (DS) of 0.284 was obtained, which was 123.62 % higher than that without of the pretreatment process. The prepared carboxymethyl starch was used to encapsulate acai berry anthocyanins (ABA) in microcapsules, and the biomedical application of the prepared carboxymethyl starch was initially attempted. This study provided a new method for preparing carboxymethyl starch and attempted to explore the application value of this product in biomedicine.

羧甲基淀粉（CMSt）是一种性能优良的变性淀粉。本研究采用碳酸钠预处理提高淀粉的羧甲基化反应活性，成功制备了中低取代度的羧甲基淀粉，并利用响应面法对羧甲基淀粉的合成参数进行了优化。结果表明，当预处理浓度为0.95 mol/L，预处理时间为54 min，碱与淀粉的摩尔比为1.3，酸与淀粉的摩尔比为1时，可制得取代度（DS）为0.284的羧甲基淀粉，比未进行预处理的羧甲基淀粉提高了123.62%。将制备的羧甲基淀粉用于巴西莓花青素（ABA）微胶囊的包封，并对制备的羧甲基淀粉在生物医学上的应用进行了初步尝试。本研究提供了一种制备羧甲基淀粉的新方法，并试图探索该产品在生物医学上的应用价值。

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引用次数: 0