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Photodynamic Therapy or Palliative Resection Versus Biliary Stenting for Advanced Cholangiocarcinoma: A Network Meta-Analysis. 光动力疗法或姑息性切除术与胆道支架植入术治疗晚期胆管癌:一项网络Meta分析。
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-11-26 DOI: 10.1089/photob.2024.0080
Sifan Dong, An Jiang, Qifan Liu, Shiqi An

Background: We assessed the efficacy of evaluating palliative resection (R1/R2 resection), photodynamic therapy (PDT), and biliary stenting (stent) alone in the treatment of advanced cholangiocarcinoma using a reticulated meta-analysis. Methods: A systematical retrieval in PubMed, Web of Science, and the Cochrane Library was performed for relative literature on the effects of PDT, palliative resection, and simple biliary stenting in the treatment of advanced cholangiocarcinoma. A literature search updated to January 30, 2024, was performed. Newcastle-Ottawa Scale and Risk Of Bias In Non-randomised Studies-of Interventions (ROBINS-I) tools were used to assess the methodological quality of the included studies, and Addis-1.16 0.6 software for reticulated meta-analysis. Results: The results showed that PDT and palliative resection were superior to biliary stenting alone in improving 1-, 2-, and 3-year survivals, and the incidence of cholangitis. The relative effectiveness of PDT, palliative resection, and biliary stent placement alone in improving survival was as follows: PDT, palliative resection, and biliary stent placement alone. Conclusion: There is no significant difference between PDT and palliative resection in improving patient survival time, and PDT does not increase the incidence of cholangitis.

背景:我们采用网状荟萃分析评估了姑息性切除术(R1/R2切除术)、光动力疗法(PDT)和单独胆道支架植入术治疗晚期胆管癌的疗效。研究方法在 PubMed、Web of Science 和 Cochrane Library 中系统检索了有关光动力疗法、姑息性切除术和单纯胆道支架术治疗晚期胆管癌效果的相关文献。文献检索更新至 2024 年 1 月 30 日。使用纽卡斯尔-渥太华量表(Newcastle-Ottawa Scale)和非随机干预研究中的偏倚风险(ROBINS-I)工具评估纳入研究的方法学质量,并使用Addis-1.16 0.6软件进行网状荟萃分析。结果显示结果显示,在提高1年、2年和3年生存率以及胆管炎发病率方面,PDT和姑息性切除术优于单纯胆道支架植入术。PDT、姑息性切除术和单纯胆道支架置入术在提高生存率方面的相对效果如下:PDT、姑息性切除术和单纯胆道支架置入术。结论光动力疗法和姑息性切除术在改善患者生存时间方面没有明显差异,而且光动力疗法不会增加胆管炎的发病率。
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引用次数: 0
Antimicrobial Photodynamic Therapy in the Treatment of Diabetic Foot Ulcer: A Scoping Review. 治疗糖尿病足溃疡的抗菌光动力疗法:范围界定综述。
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-11-25 DOI: 10.1089/photob.2024.0013
Maria Girlane Sousa Albuquerque Brandão, Soraia Assad Nasbine Rabeh

Objective: To map the literature about photodynamic therapy in treating diabetic foot ulcers. Background: Diabetic foot ulcers get constantly infected, thus culminating in hospitalizations and amputations. Photodynamic therapy is an antimicrobial treatment that may assist in the healing process. Materials and Methods: A search of nine electronic information sources was made as determined by the Joanna Briggs Institute. Two independent researchers accomplished a screening of studies with the support of Rayyan. The data were analyzed through Iramutec®. Results: The sample consisted of 27 studies. Photodynamic therapy was identified as safe and effective, with the ability to reduce pain, edema, exudate, extent of the injury region, microbial load, and the risks of infection, osteomyelitis, and amputations. Conclusions: The capacity of photodynamic therapy to relieve symptoms, decrease risks of complications, and accelerate the healing process highlights its potential positive impact on clinical practice.

目的:了解有关光动力疗法治疗糖尿病足溃疡的文献。背景:糖尿病足溃疡经常受到感染,最终导致住院和截肢。光动力疗法是一种抗菌治疗方法,可帮助溃疡愈合。材料与方法:根据乔安娜-布里格斯研究所(Joanna Briggs Institute)的规定,对九个电子信息来源进行了搜索。在瑞扬公司的支持下,两名独立研究人员完成了研究筛选。数据通过 Iramutec® 进行分析。结果样本包括 27 项研究。光动力疗法被认为安全有效,能够减轻疼痛、水肿、渗出、损伤区域范围、微生物负荷以及感染、骨髓炎和截肢的风险。结论光动力疗法能够缓解症状、降低并发症风险并加速愈合过程,这凸显了其对临床实践的潜在积极影响。
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引用次数: 0
Comparative Study of Photobiomodulation Effects on Alveolar Socket Hard Tissue Healing in Rats: Application of 980 nm Versus 810 nm Lasers. 光生物调节对大鼠牙槽窝硬组织愈合影响的比较研究:980 纳米与 810 纳米激光的应用。
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-10-18 DOI: 10.1089/photob.2024.0058
Boxi Fan, Bo Shao, Shouyin Lv, Xu Liu, Jiang Sui, Congjia Bai, Wenlong Zhang, Shengzhao Xiao, Xiao-Hui Jiao

Background: This study aimed to explore the differential effects of photobiomodulation (PBM) via 980 nm and 810 nm lasers on the hard tissue healing of rat alveolar sockets, with a focus on a comparative analysis of hard tissue regeneration and osteogenic gene expression. Objective: This study aimed to explore the effects of PBM using 980 nm and 810 nm lasers on hard tissue healing of rat alveolar sockets, focusing on hard tissue regeneration and osteogenic gene expression. Materials and Methods: Thirty-six male Wistar rats (5 weeks old) had both right and left maxillary first molars extracted. Post extraction, the right alveolar sockets received PBM treatment with either 980 nm (0.3 W, 18 J/cm2) or 810 nm (0.1 W, 6 J/cm2) lasers for seven days, whereas the left sockets served as controls. Rats were euthanized on days 3, 7, 14, and 28 for histopathological, immunohistochemical, micro computed tomography (micro-CT), and quantitative polymerase chain reactionanalyses. Results: On day 3, early granulation tissue, neovascularization, and inflammatory cell aggregates were observed in all groups. By day 7, active osteoclasts and osteoblasts were noted, with a significant increase in CD31-positive cells in the 980 nm group (p < 0.05). Day 14 showed new bone formation, and by day 28, increased cancellous bone and collagen content were present in all groups, with no significant differences between them (p > 0.05). Gene expression analysis revealed higher BMP-2 and Runx-2 levels in laser-treated groups on day 14 (p < 0.05), with the 980 nm group having higher BMP-2 levels than the 810 nm group (p < 0.05). Bone sialoprotein expression was higher in laser-treated groups on days 14 and 28 (p < 0.05), and osteocalcin expression was highest in the 980 nm group on both days (p < 0.05). Micro-CT analysis showed no significant differences among groups in bone mineral density, bone surface (BS)/bone volume (BV), or bone volume (BV)/TV (total volume) indices. Conclusion: PBM with 980 nm and 810 nm lasers promotes early-stage hard tissue healing in extraction sockets, with the 980 nm laser more effectively enhancing osteogenic gene expression, suggesting its potential as an adjunctive therapy in dental and oral surgery.

背景:本研究旨在探讨通过 980 纳米和 810 纳米激光进行光生物调节(PBM)对大鼠牙槽窝硬组织愈合的不同影响,重点是对硬组织再生和成骨基因表达进行比较分析。研究目的本研究旨在探讨使用 980 纳米和 810 纳米激光进行 PBM 对大鼠牙槽窝硬组织愈合的影响,重点关注硬组织再生和成骨基因表达。材料与方法:36 只雄性 Wistar 大鼠(5 周大)拔除了左右上颌第一磨牙。拔牙后,右侧牙槽窝接受 980 nm(0.3 W,18 J/cm2)或 810 nm(0.1 W,6 J/cm2)激光的 PBM 治疗,为期七天,而左侧牙槽窝作为对照。大鼠在第 3、7、14 和 28 天安乐死,进行组织病理学、免疫组织化学、微型计算机断层扫描 (micro-CT) 和定量聚合酶链反应分析。结果显示第 3 天,各组均观察到早期肉芽组织、新生血管和炎性细胞聚集。第 7 天,发现破骨细胞和成骨细胞活跃,980 纳米组 CD31 阳性细胞显著增加(p < 0.05)。第 14 天显示有新骨形成,到第 28 天,所有组的松质骨和胶原蛋白含量都有所增加,组间差异不显著(p > 0.05)。基因表达分析显示,第 14 天,激光治疗组的 BMP-2 和 Runx-2 水平更高(p < 0.05),其中 980 纳米组的 BMP-2 水平高于 810 纳米组(p < 0.05)。激光治疗组在第 14 天和第 28 天的骨硅蛋白表达量较高(p < 0.05),980 纳米组在这两天的骨钙素表达量最高(p < 0.05)。显微 CT 分析表明,各组在骨矿物质密度、骨表面 (BS)/ 骨体积 (BV) 或骨体积 (BV)/TV (总体积)指数方面无明显差异。结论使用 980 nm 和 810 nm 激光进行 PBM 可促进拔牙窝早期硬组织愈合,其中 980 nm 激光能更有效地增强成骨基因的表达,这表明它有可能成为牙科和口腔外科的一种辅助疗法。
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引用次数: 0
Efficacy of Laser Acupuncture on Neurophysiological Parameters of Median Nerve and Hand Function in Postpartum Women: A Randomized Controlled Clinical Trial. 激光针灸对产后妇女正中神经的神经电生理参数和手部功能的疗效:随机对照临床试验》。
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-10-24 DOI: 10.1089/photob.2024.0106
Afaf M Botla, Yasser R Lasheen, Shereen S Mohamed, Reem M Alwhaibi, Hoda M Zakaria, Walaa M Ragab, Manal A El-Shafei

Objective: This study was conducted to ascertain the efficacy of photo acupuncture (PA) on the neurophysiological parameters of the median nerve and hand function in postnatal women. Background: Carpal tunnel syndrome (CTS) has been documented in up to 62% of pregnant females. PA, a noninvasive treatment that utilizes photo therapy on acupoints, could aid in the management of CTS. Material and Methods: Thirty-six postpartum women diagnosed with CTS depending on their medical history, physical examination, and electrodiagnostic study were distributed into two equal-sized groups at random. The PA group (n = 18) received PA for 12 min on the following acupuncture points: PC4, PC6, PC7, PC8, HT2, HT3, HT7, LI10, LI11, LI4, LU9, and LU10. The treatment was given 3 times weekly for four weeks; in addition, night splints were worn. Women in the control group (n = 18) just wore night splints. Before and after the 12-session treatment, each woman was assessed using an electrodiagnostic test to measure the motor distal latency (MDL), sensory distal latency (SDL), motor conduction velocity (MCV), sensory conduction velocity (SCV), pinch dynamometer for assessing pinch strength, and the Brief Michigan Hand Questionnaire (BMHQ) for assessing hand function. Results: A statistically significant improvement was observed in both groups after therapy regarding SDL, SCV, MDL, MCV, pinch strength, and BMHQ (p < 0.05) in favor of the PA group. Conclusion: Adding PA to a night splint should be suggested as an effective conservative therapy for CTS in postpartum women.

研究目的本研究旨在确定光针灸(PA)对产后妇女正中神经的神经生理参数和手部功能的疗效。研究背景据记录,高达 62% 的孕妇患有腕管综合征 (CTS)。PA是一种利用光疗法对穴位进行治疗的非侵入性疗法,可帮助治疗腕管综合征。材料与方法:将根据病史、体格检查和电诊断研究确诊为 CTS 的 36 名产后妇女随机分为两组,每组人数相等。PA 组(n = 18)在以下穴位上接受 12 分钟的 PA 治疗:PC4、PC6、PC7、PC8、HT2、HT3、HT7、LI10、LI11、LI4、LU9 和 LU10。治疗每周 3 次,持续 4 周;此外,还佩戴了夜间夹板。对照组(18 人)的妇女只佩戴夜间夹板。在接受 12 个疗程的治疗前后,每位妇女都接受了电诊断测试,以测量运动远端潜伏期 (MDL)、感觉远端潜伏期 (SDL)、运动传导速度 (MCV)、感觉传导速度 (SCV)、评估捏力的捏力计以及评估手部功能的密歇根手部简明问卷 (BMHQ)。结果显示治疗后,两组患者在SDL、SCV、MDL、MCV、捏力和BMHQ方面均有明显改善(P < 0.05),PA组更明显。结论建议在夜间夹板中加入 PA,作为治疗产后女性 CTS 的有效保守疗法。
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引用次数: 0
NIR Laser Irradiation Promotes Osteogenic Differentiation of PDLSCs Through the Activation of TRPV1 Channels and Subsequent Calcium Signaling. 近红外激光照射通过激活 TRPV1 通道和随后的钙信号转导促进 PDLSCs 的成骨分化
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-09-24 DOI: 10.1089/photob.2024.0061
Jia-Hao Zeng, Bing'er Ma, Xiao-Qing Shen, Yuan-Ming Geng

Near-infrared (NIR) irradiation has shown potential to stimulate osteogenic differentiation, but the mechanisms are not fully understood. The study is to investigate the effects of NIR laser irradiation on osteoblastic differentiation. Human periodontal ligament stem cells (hPDLSCs) were cultured in osteogenic medium and exposed to 810 nm NIR laser at 0.5 J/cm2 every 48 h. The transient receptor potential vanilloid (TRPV1) channel inhibitor capsazepine (CPZ) was used to evaluate the role of calcium influx. Osteogenic differentiation was assessed by proliferation (CCK-8), alkaline phosphatase (ALP) activity, mineralization (Alizarin Red), and expression of bone markers by PCR and Western blot over 2 weeks. Intracellular calcium was measured by Fluo-4M dye and flow cytometry. Results showed that NIR irradiation enhanced hPDLSC proliferation, ALP activity, mineralization, and bone marker expression, indicating increased osteogenic differentiation. These effects were inhibited by CPZ. NIR induced a transient rise in intracellular calcium peaking at 3 min, which was blocked by CPZ. In conclusion, this study demonstrates that NIR laser irradiation promotes osteogenic differentiation of PDLSCs through the activation of TRPV1 channels and subsequent calcium signaling. Further research is warranted to optimize the treatment parameters and elucidate the detailed signaling pathways involved, paving the way for the clinical application of NIR therapy in the treatment of bone disorders and periodontal disease.

近红外(NIR)辐照具有刺激成骨细胞分化的潜力,但其机制尚不完全清楚。本研究旨在探讨近红外激光照射对成骨细胞分化的影响。将人牙周韧带干细胞(hPDLSCs)培养在成骨培养基中,每 48 小时暴露于 0.5 J/cm2 的 810 nm 近红外激光。在两周内,通过增殖(CCK-8)、碱性磷酸酶(ALP)活性、矿化(茜素红)以及通过 PCR 和 Western 印迹检测骨标志物的表达来评估成骨分化。细胞内钙通过 Fluo-4M 染料和流式细胞术进行测量。结果表明,近红外照射增强了 hPDLSC 的增殖、ALP 活性、矿化和骨标记表达,表明成骨分化增强。CPZ 可抑制这些效应。近红外诱导细胞内钙一过性升高,在 3 分钟达到峰值,CPZ 可阻断这种升高。总之,本研究表明,近红外激光照射通过激活 TRPV1 通道和随后的钙信号转导促进了 PDLSCs 的成骨分化。我们需要进一步研究,优化治疗参数,阐明其中涉及的详细信号通路,为近红外疗法在骨病和牙周病治疗中的临床应用铺平道路。
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引用次数: 0
The Biofilm Removal and Bactericidal Effect of an 810-nm High-Power Laser on an Orthodontic Bracket Surface: An In Vitro Study. 810 纳米高功率激光对正畸托槽表面生物膜的去除和杀菌作用:体外研究
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-10-23 DOI: 10.1089/photob.2024.0088
Huan Yang, Kai Yang
<p><p><b><i>Objective:</i></b> The present study aimed to analyze the biofilm removal and bactericidal effect of laser treatment alone and laser combined with ultrasonic scaling on orthodontic brackets. It also assessed whether the use of a laser can improve the efficiency of biofilm removal and bactericidal effect compared with traditional ultrasonic instrumentation. <b><i>Background Data:</i></b> <i>Streptococcus mutans</i> (<i>S. mutans</i>) can lead to white spots and dental caries. Orthodontic brackets make teeth cleaning more difficult, and biofilms or bacteria on the surface of brackets worsen the oral environment, which may cause some oral diseases. Laser can be used for biofilm removal and killing bacteria on the surface of an object through thermal, photochemical, and pressure effects, which is widely used in the treatment of oral diseases. <b><i>Methods:</i></b> A total of 600 mandibular incisor brackets were collected for this study. Among these, 320 unused brackets were used for the <i>S. mutans</i> crystal violet assay (<i>n</i> = 160) and for <i>S. mutans</i> live/dead bacterial staining (<i>n</i> = 160). Another 280 brackets, obtained from patients who had undergone therapy for over two years, were used for the mature multispecies biofilms removal assay (<i>n</i> = 120) and multispecies bacterial live/dead bacterial staining (<i>n</i> = 160). Ultrasonic scaling, laser, and laser combined with ultrasonic scaling were applied to the labial surface of brackets covered by <i>S. mutans</i> biofilm or mature multispecies biofilms. Specifically, we used the following three methods: ultrasonic scaling for 10 sec without laser; 810-nm laser (Doctor Smile, Italy, LA5D0 001.1) with 0.3-mm spot size at total 21.2 kJ/cm<sup>2</sup> for 10 sec; and 810-nm laser at total 10.6 kJ/cm<sup>2</sup> for 5 sec, followed by ultrasonic scaling for 5 sec. The 810-nm diode laser removed biofilms with a power of 1.5 W and a power density of 2.12 kW/cm<sup>2</sup>. The <i>S. mutans</i> biofilm was examined using crystal violet assay, and scanning electron microscopy (SEM) was used for mature multispecies biofilms to evaluate the effect of the three methods on biofilm removal. Live/dead bacterial staining was used to examine the bactericidal effect on remaining biofilms by confocal laser scanning microscopy (CLSM). <b><i>Results:</i></b> For <i>S. mutans</i> biofilm, the optical density (OD) value and live/dead bacterial ratio in the laser and the laser combined with ultrasonic scaling groups were significantly lower than those in the ultrasonic scaling group (<i>p</i> < 0.05); moreover, the OD value and the live/dead bacterial ratio in laser treatment combined with ultrasonic scaling and laser treatment alone showed no significant difference (<i>p</i> > 0.05). For mature multispecies biofilms, the percentage of biofilm coverage after treatment was higher in the laser group than in the ultrasonic scaling group (<i>p</i> < 0.05) and lower in the laser combined
研究目的本研究旨在分析单独使用激光治疗和激光与超声波洗牙相结合对正畸托槽的生物膜去除和杀菌效果。与传统的超声波器械相比,本研究还评估了使用激光是否能提高生物膜去除效率和杀菌效果。背景数据:变异链球菌(S. mutans)可导致白斑和龋齿。正畸托槽会增加牙齿清洁的难度,托槽表面的生物膜或细菌会使口腔环境恶化,从而引发一些口腔疾病。激光可用于清除生物膜,通过热效应、光化学效应和压力效应杀死物体表面的细菌,在口腔疾病治疗中得到广泛应用。方法:本研究共收集了 600 个下颌门牙托槽。其中,320 个未使用的托槽被用于变异杆菌水晶紫检测(160 个)和变异杆菌活/死细菌染色(160 个)。另外 280 个托架来自接受过两年以上治疗的患者,用于成熟多菌种生物膜去除试验(n = 120)和多菌种细菌活/死细菌染色(n = 160)。超声波洗牙、激光洗牙以及激光与超声波洗牙相结合的方法被应用于被变异棒状杆菌生物膜或成熟的多菌种生物膜覆盖的托槽唇面。具体来说,我们使用了以下三种方法:超声波洗牙 10 秒,不使用激光;810 纳米激光(意大利 Doctor Smile 公司,LA5D0 001.1),光斑大小为 0.3 毫米,总能量为 21.2 千焦/平方厘米,持续 10 秒;810 纳米激光,总能量为 10.6 千焦/平方厘米,持续 5 秒,然后超声波洗牙 5 秒。810 纳米二极管激光去除生物膜的功率为 1.5 W,功率密度为 2.12 kW/cm2。使用结晶紫检测法检查变异单胞菌生物膜,并使用扫描电子显微镜(SEM)观察成熟的多菌种生物膜,以评估三种方法对生物膜去除的影响。利用共焦激光扫描显微镜(CLSM)对活/死细菌染色,检查对残留生物膜的杀菌效果。结果对于变异单胞菌生物膜,激光组和激光联合超声波洗牙组的光密度(OD)值和活/死菌比均显著低于超声波洗牙组(P < 0.05);此外,激光治疗联合超声波洗牙组和单独激光治疗组的光密度(OD)值和活/死菌比无显著差异(P > 0.05)。对于成熟的多菌种生物膜,激光组治疗后生物膜覆盖率高于超声波洁治组(P < 0.05),激光联合超声波洁治组低于超声波洁治组(P < 0.05),活/死细菌染色显示,单独激光治疗杀死的细菌最多,其次是激光治疗联合超声波洁治,而单独超声波洁治很少杀死细菌。结论是单独使用激光治疗比单独使用超声波洁牙有更好的杀菌效果,也能去除更多的变异牙齿螺杆菌生物膜,但无法去除更成熟的多菌种生物膜。激光治疗结合超声波洁牙比单独使用超声波洁牙能去除更多的变异杆菌生物膜和成熟的多菌种生物膜,而且比单独使用超声波洁牙对托槽表面的杀菌效果更好。
{"title":"The Biofilm Removal and Bactericidal Effect of an 810-nm High-Power Laser on an Orthodontic Bracket Surface: An In Vitro Study.","authors":"Huan Yang, Kai Yang","doi":"10.1089/photob.2024.0088","DOIUrl":"10.1089/photob.2024.0088","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;&lt;i&gt;Objective:&lt;/i&gt;&lt;/b&gt; The present study aimed to analyze the biofilm removal and bactericidal effect of laser treatment alone and laser combined with ultrasonic scaling on orthodontic brackets. It also assessed whether the use of a laser can improve the efficiency of biofilm removal and bactericidal effect compared with traditional ultrasonic instrumentation. &lt;b&gt;&lt;i&gt;Background Data:&lt;/i&gt;&lt;/b&gt; &lt;i&gt;Streptococcus mutans&lt;/i&gt; (&lt;i&gt;S. mutans&lt;/i&gt;) can lead to white spots and dental caries. Orthodontic brackets make teeth cleaning more difficult, and biofilms or bacteria on the surface of brackets worsen the oral environment, which may cause some oral diseases. Laser can be used for biofilm removal and killing bacteria on the surface of an object through thermal, photochemical, and pressure effects, which is widely used in the treatment of oral diseases. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; A total of 600 mandibular incisor brackets were collected for this study. Among these, 320 unused brackets were used for the &lt;i&gt;S. mutans&lt;/i&gt; crystal violet assay (&lt;i&gt;n&lt;/i&gt; = 160) and for &lt;i&gt;S. mutans&lt;/i&gt; live/dead bacterial staining (&lt;i&gt;n&lt;/i&gt; = 160). Another 280 brackets, obtained from patients who had undergone therapy for over two years, were used for the mature multispecies biofilms removal assay (&lt;i&gt;n&lt;/i&gt; = 120) and multispecies bacterial live/dead bacterial staining (&lt;i&gt;n&lt;/i&gt; = 160). Ultrasonic scaling, laser, and laser combined with ultrasonic scaling were applied to the labial surface of brackets covered by &lt;i&gt;S. mutans&lt;/i&gt; biofilm or mature multispecies biofilms. Specifically, we used the following three methods: ultrasonic scaling for 10 sec without laser; 810-nm laser (Doctor Smile, Italy, LA5D0 001.1) with 0.3-mm spot size at total 21.2 kJ/cm&lt;sup&gt;2&lt;/sup&gt; for 10 sec; and 810-nm laser at total 10.6 kJ/cm&lt;sup&gt;2&lt;/sup&gt; for 5 sec, followed by ultrasonic scaling for 5 sec. The 810-nm diode laser removed biofilms with a power of 1.5 W and a power density of 2.12 kW/cm&lt;sup&gt;2&lt;/sup&gt;. The &lt;i&gt;S. mutans&lt;/i&gt; biofilm was examined using crystal violet assay, and scanning electron microscopy (SEM) was used for mature multispecies biofilms to evaluate the effect of the three methods on biofilm removal. Live/dead bacterial staining was used to examine the bactericidal effect on remaining biofilms by confocal laser scanning microscopy (CLSM). &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; For &lt;i&gt;S. mutans&lt;/i&gt; biofilm, the optical density (OD) value and live/dead bacterial ratio in the laser and the laser combined with ultrasonic scaling groups were significantly lower than those in the ultrasonic scaling group (&lt;i&gt;p&lt;/i&gt; &lt; 0.05); moreover, the OD value and the live/dead bacterial ratio in laser treatment combined with ultrasonic scaling and laser treatment alone showed no significant difference (&lt;i&gt;p&lt;/i&gt; &gt; 0.05). For mature multispecies biofilms, the percentage of biofilm coverage after treatment was higher in the laser group than in the ultrasonic scaling group (&lt;i&gt;p&lt;/i&gt; &lt; 0.05) and lower in the laser combined","PeriodicalId":94169,"journal":{"name":"Photobiomodulation, photomedicine, and laser surgery","volume":" ","pages":"779-788"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photobiomodulation and Physical Exercise Modulate of Cell Survival Proteins in the Skeletal Muscle of Rats with Heart Failure and Diabetes Mellitus. 光生物调节和体育锻炼可调节心力衰竭和糖尿病大鼠骨骼肌中的细胞存活蛋白
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-10-23 DOI: 10.1089/photob.2024.0073
Alan Christhian Bahr, Naira Bohrer Scherer, Elizama de Gregório, Lucas Kieling, Alexandre Luz de Castro, Alex Sander da Rosa Araujo, Patrick Türck, Pedro Dal Lago

Introduction: Heart failure (HF) and type 2 diabetes mellitus (DM2) are global health problems that often lead to muscle atrophy. These conditions are associated with increased autophagy and apoptosis in the muscle cells, resulting in decreased muscle mass. Physical exercise associated with photobiomodulation (PBM) seems promising to attenuate the skeletal muscle changes caused by HF and DM2, due to its direct effects on mitochondria, which may result in an increase in antioxidant capacity. Objective: To verify the influence of physical exercise and the association with PBM on autophagy, apoptosis, and cell survival signaling pathways in myocytes from rats with HF and DM2. Materials and Methods: Male rats were assigned to one of four groups: control (CT), HF+DM (disease model), exercise+HF+DM (EX+HF+DM), and EX+HF+DM+PBM (EX+HF+DM+PBM). To induce DM2, we administered streptozotocin (STZ) (0.25 mL/kg, intraperitoneally). HF was induced by coronary ligation. One week post-induction, an 8-week aerobic exercise and PBM protocol was initiated. Western blot analysis was used to measure the expression of apoptosis-related proteins and autophagy. Results: The EX+HF+DM+PBM group showed a substantial increase in Nrf2, p-AKT, and LC3-I levels compared to the HF+DM group. Conclusions: These findings suggest that physical exercise combined with PBM can upregulate proteins that promote myocyte survival in rats with HF and DM2.

简介心力衰竭(HF)和 2 型糖尿病(DM2)是经常导致肌肉萎缩的全球性健康问题。这些疾病与肌肉细胞自噬和凋亡增加有关,导致肌肉质量下降。与光生物调节(PBM)相关的体育锻炼似乎有望减轻高血脂和糖尿病引起的骨骼肌变化,这是因为光生物调节对线粒体有直接影响,可提高抗氧化能力。研究目的验证体育锻炼和 PBM 对高血脂和 DM2 大鼠肌细胞自噬、细胞凋亡和细胞存活信号通路的影响。材料和方法:雄性大鼠分为四组:对照组(CT)、HF+DM 组(疾病模型)、运动+HF+DM 组(EX+HF+DM)和 EX+HF+DM+PBM 组(EX+HF+DM+PBM)。为了诱导DM2,我们注射了链脲佐菌素(STZ)(0.25 mL/kg,腹腔注射)。通过冠状动脉结扎诱导高血压。诱导一周后,开始为期8周的有氧运动和PBM方案。采用 Western 印迹分析法检测细胞凋亡相关蛋白和自噬的表达。结果显示EX+HF+DM+PBM组与HF+DM组相比,Nrf2、p-AKT和LC3-I水平显著增加。结论这些研究结果表明,体育锻炼结合 PBM 可上调促进 HF 和 DM2 大鼠肌细胞存活的蛋白质。
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引用次数: 0
The Effects of Photobiomodulation Therapy on Tensile-Cultured Cementoblasts Cells. 光生物调节疗法对拉伸培养的水门母细胞的影响
IF 1.8 Q2 SURGERY Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI: 10.1089/photob.2024.0076
Chuan-Yi Kao, Chun-Te Ho, Tsui-Hsein Huang, Chia-Tze Kao

Background: Studies show that photobiomodulation therapy (PBMT) boosts cellular ATP production and cell growth and reduces inflammation. Additionally, mechanical tension affects gene expression, impacting cellular functions like proliferation and migration. Objective: We investigated the impact of PBMT on OCCM-30 cementoblast cells under tensile stress, focusing on cell survival, differentiation, and inflammatory responses, particularly relating to orthodontic tooth movement and root resorption. Methods: Cultured OCCM-30 cells under negative pressure received PBMT with a 10.6 μm wavelength in continuous mode at 1.0 W power for 3, 5, or 10 sec, corresponding to energy densities of 3, 5, or 10 J/cm2. We assessed cell viability with the Presto Blue assay and inflammatory markers Interleukin 6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) through western blots at 1, 12, 24 h, and 7 days post-irradiation. Results: PBMT improved cell viability over time while maintaining levels of inflammatory markers. alkaline phosphatase levels dropped initially but increased after 7 days, suggesting enhanced cementoblast differentiation. IL-6 levels rose gradually, with 3J and 5J treatments showing significantly higher levels than the control. iNOS levels spiked within the first 24 h, then declined by day 7. COX-2 levels consistently rose, with the 5J treatment showing greater increases. Conclusions: PBMT appears to support cementoblast survival and differentiation while managing inflammation, potentially aiding root repair during orthodontic treatments and reducing inflammatory root resorption.

背景:研究表明,光生物调控疗法(PBMT)可促进细胞 ATP 的产生和细胞生长,并减少炎症。此外,机械张力会影响基因表达,从而影响增殖和迁移等细胞功能。研究目的我们研究了 PBMT 对拉伸应力下 OCCM-30 骨水泥母细胞的影响,重点关注细胞存活、分化和炎症反应,尤其是与正畸牙齿移动和牙根吸收有关的方面。研究方法在负压下培养的 OCCM-30 细胞接受波长为 10.6 μm 的连续模式 PBMT,功率为 1.0 W,时间为 3、5 或 10 秒,能量密度分别为 3、5 或 10 J/cm2。我们用 Presto Blue 检测法评估细胞存活率,并在辐照后 1、12、24 和 7 天通过 Western 印迹评估炎症标志物白细胞介素 6(IL-6)、诱导型一氧化氮合酶(iNOS)和环氧化酶-2(COX-2)。结果显示碱性磷酸酶水平最初有所下降,但在 7 天后有所上升,这表明骨水泥母细胞分化得到加强。IL-6 水平逐渐升高,3J 和 5J 处理的水平明显高于对照组。COX-2 水平持续上升,5J 处理的升幅更大。结论:PBMT 似乎在控制炎症的同时支持骨水泥母细胞的存活和分化,有可能在正畸治疗期间帮助牙根修复并减少炎症性牙根吸收。
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引用次数: 0
The Effects of Photobiomodulation Therapy in Cubital Tunnel Syndrome, Clinical Trial. 光生物调制疗法对拇指管综合征的影响,临床试验。
IF 1.8 Q2 SURGERY Pub Date : 2024-11-01 Epub Date: 2024-10-14 DOI: 10.1089/photob.2024.0089
Gurbet Yağcı, Meliha Kasapoğlu Aksoy, İlknur Aykurt Karlıbel, Meral Seferoğlu

Aim: We aimed to evaluate the effects of laser therapy together with rest splint and exercise on muscle strength, function, activities of daily living, and pain in individuals suffering from cubital tunnel syndrome (CuTS) by comparing it with placebo treatment. Materials and Methods: This prospective, single-blind, and randomized controlled study was conducted with 64 patients with CuTS in total. They were divided into two groups by randomization: Group 1 (n = 32) was provided with low-energy laser therapy + splint + exercise, and Group 2 (n = 32) was provided with low-energy sham laser therapy + splint + exercise. Patients in both groups received resting splints and nerve release exercises. Pretreatment and posttreatment (3rd week, 3rd month) evaluations were made with the Visual Analog Scale (VAS), the Quick Disability of Arm, Shoulder, and Hand Questionnaire, hand grip strength, fingertip grip strength, the Patient-Rated Ulnar Nerve Evaluation Scale (PRUNES), and Short Form-12. An electrophysiological evaluation was performed at baseline and at the 3rd month. Results: We observed significant improvements in both groups regarding pain, function, muscle strength, health profile, and quality of life in the early posttreatment (3rd week) stage and at the 3rd-month follow-up. When both groups were compared, a significant difference was determined between the VAS-Rest, VAS-Movement, PRUNES-Pain, PRUNES-Function, and PRUNES-Total parameters in the posttreatment (3rd week follow-ups), as well as PRUNES-Pain scores in the post-treatment (3rd month) stage, of the groups in favor of those in Group 1 (p < 0.05). Conclusion: The findings of our study have shown that in the treatment of CuTS, laser treatment is superior to placebo in the short term, but they have equivalent effects in the medium term. Further studies with large patient populations are needed to provide more diverse information about the therapeutic effectiveness of low-intensity laser therapy.

目的:通过与安慰剂治疗进行比较,我们旨在评估激光疗法与休息夹板和运动对肘管综合征(CuTS)患者的肌力、功能、日常生活活动和疼痛的影响。材料与方法:这项前瞻性、单盲、随机对照研究共纳入了 64 名 CuTS 患者。他们被随机分为两组:第一组(32 人)接受低能量激光治疗+夹板+运动,第二组(32 人)接受低能量假激光治疗+夹板+运动。两组患者均接受休息夹板和神经松解运动。治疗前和治疗后(第3周、第3个月)的评估包括视觉模拟量表(VAS)、手臂、肩部和手部快速残疾问卷、手部握力、指尖握力、患者评定的尺神经评估量表(PRUNES)和简表-12。在基线和第 3 个月时进行了电生理评估。结果:在治疗后早期(第 3 周)和第 3 个月的随访中,我们观察到两组患者在疼痛、功能、肌力、健康状况和生活质量方面均有明显改善。两组患者在治疗后(第 3 周随访)的 VAS-休息、VAS-运动、PRUNES-疼痛、PRUNES-功能和 PRUNES-总分参数以及治疗后(第 3 个月)的 PRUNES-疼痛评分之间存在显著差异,第一组患者更优(P < 0.05)。结论我们的研究结果表明,在治疗 CuTS 时,激光治疗的短期疗效优于安慰剂,但两者的中期疗效相当。需要对大量患者进行进一步研究,以提供有关低强度激光疗法疗效的更多信息。
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引用次数: 0
Effects of Laser Acupuncture on Metabolic Functions of Sedentary People: A Double-Blind Randomized Clinical Trial. 激光针灸对久坐人群代谢功能的影响:双盲随机临床试验。
IF 1.8 Q2 SURGERY Pub Date : 2024-11-01 Epub Date: 2024-09-30 DOI: 10.1089/photob.2023.0162
Henrique Manoel de Araujo Martins Filho, Melissa de Almeida Melo Maciel Mangueira, Luciano Gonçalves da Nóbrega, Ozimo Pereira Gama Filho, Osmar Pinto Neto, Nilton Maciel Mangueira, Renato Amaro Zângaro

Background: Laser acupuncture regulates energy flow and restores body fluid metabolism. Objective: To evaluate the effects of the laser acupuncture protocol (LAP) on hepatic and renal metabolism in sedentary people. Methods: Longitudinal, double-blind, and randomized clinical trial with 29 participants, adults, both sexes, sedentary, without pre-existing metabolic diseases, subdivided into control and laser groups. Based on the STandards for Reporting Interventions in Clinical Trials of Acupuncture 2010 guidelines, 10 laser applications (660 nm ±10 nm wavelength, 100 mW power. The irradiation tip has a diameter of 5 mm, which corresponds to an area of 0.19 cm2, totaling a power density of 0.52 W/cm2 and considering the irradiation time of 90 s, the energy density applied was 47.3 J/cm2) were performed on the acupoints of metabolic functions (LR3, SP6, ST36, and LI4) and blood samples were collected for fasting glycemia, lipid profile (HDL, LDL, total cholesterol, and triglycerides), liver function (AST/GOT and ALT/GPT), and renal function (serum creatinine and urea). A repeated measures analysis of variance (ANOVA) with Bonferroni corrected post hoc comparisons was applied to compare statistical differences between groups and times, adopting p < 0.05 as the null hypothesis. Results: The laser stimulated changes in serum lipid profile values and renal and hepatic functions. There was a significant (p = 0.014) reduction in LDL ("bad" cholesterol) from 105.75 ± 32.83 pre- to 84.32 ± 18.38 mg/dL postintervention, associated with cardioprotective function. Positive significant (p = 0.035) impacts were also observed in the reduction of creatinine (0.86 ± 0.12 mg/dL to 0.75 ± 0.12 mg/dL) and the enzyme AST/GOT (33.73 ± 12.95 U/L to 20.80 ± 4.99 U/L, p = 0.002). Conclusion: LAP applied to basal metabolism acupoints promoted positive metabolic changes in the lipid profile (LDL), and in main markers of the liver (AST/GOT) and kidney (creatinine) functions, contributing to risk control of cardiovascular diseases.

背景介绍激光针灸可调节能量流动,恢复体液代谢。研究目的评估激光针灸方案(LAP)对久坐人群肝肾代谢的影响。方法:采用纵向、双盲、对照组和对照组方法:纵向、双盲、随机临床试验,29 名参与者均为成年人,男女不限,久坐不动,无代谢性疾病,分为对照组和激光组。根据《2010 年针灸临床试验干预措施报告标准》,10 次激光照射(波长为 660 nm ±10 nm,功率为 100 mW。照射尖端直径为 5 毫米,相当于 0.19 平方厘米的面积,总功率密度为 0.52 瓦/平方厘米,考虑到照射时间为 90 秒,应用的能量密度为 47.在新陈代谢功能穴位(LR3、SP6、ST36 和 LI4)上进行了 "穴位刺激",并采集了血样,以检测空腹血糖、血脂(高密度脂蛋白、低密度脂蛋白、总胆固醇和甘油三酯)、肝功能(AST/GOT 和 ALT/GPT)和肾功能(血清肌酐和尿素)。采用重复测量方差分析(ANOVA)和经 Bonferroni 校正的事后比较来比较组间和时间间的统计学差异,以 p < 0.05 为零假设。结果激光刺激了血清脂质概况值和肝肾功能的变化。低密度脂蛋白("坏 "胆固醇)从干预前的 105.75 ± 32.83 mg/dL 降至干预后的 84.32 ± 18.38 mg/dL,降幅明显(p = 0.014),这与心脏保护功能有关。在降低肌酐(0.86 ± 0.12 mg/dL 至 0.75 ± 0.12 mg/dL)和 AST/GOT 酶(33.73 ± 12.95 U/L 至 20.80 ± 4.99 U/L, p = 0.002)方面也观察到了积极的重大影响(p = 0.035)。结论将 LAP 应用于基础代谢穴位可促进血脂(低密度脂蛋白)、肝脏(AST/GOT)和肾脏(肌酐)功能的主要指标发生积极的代谢变化,有助于控制心血管疾病的风险。
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引用次数: 0
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Photobiomodulation, photomedicine, and laser surgery
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