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Electrode scalp impedance differences between electroencephalography machines in healthy dogs. 健康犬脑电图仪电极头皮阻抗差异。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Julia Luca, Michal Hazenfratz, Gabrielle Monteith, Andrea Sanchez, Luis Gaitero, Fiona James

Scalp electrode impedance measurements recorded by wired and wireless electroencephalography (EEG) machines in 7 healthy dogs were compared. Eight recordings resulted in 80 impedance readings from subdermal wire electrodes (locations F7/F8, F3/F4, T3/T4, C3/C4, Fz, and Cz). Impedance values were measured first from the wired and then the wireless EEG machine. Wireless impedance measurements were higher than the wired EEG machine in 79/80 readings (P ≤ 0.05), being on average 2.83 kΩ [P ≤ 0.05, 95% confidence interval (CI): 2.51 to 3.14, SD = 1.42] higher. Impedances from the wired machine ranged between < 0.5 and 9 kΩ (mean = 3.09, median = 2.00, SD = 2.15), whereas impedances from the wireless machine ranged between 2.69 and 6.07 kΩ (mean = 5.92, median = 5.05, SD = 2.59). Despite these differences in impedance measurements, both machines measured similar impedance patterns. The wireless EEG machine's impedance measurements, therefore, should be acceptable for veterinary clinical settings.

比较了7只健康犬的有线和无线脑电图(EEG)记录的头皮电极阻抗。8个记录产生了80个皮下导线电极(位置F7/F8, F3/F4, T3/T4, C3/C4, Fz和Cz)的阻抗读数。先测量有线脑电图机的阻抗值,再测量无线脑电图机的阻抗值。在79/80个读数中,无线阻抗测量值高于有线脑电图机(P≤0.05),平均为2.83 kΩ [P≤0.05,95%可信区间(CI): 2.51 ~ 3.14, SD = 1.42]。来自有线机器的阻抗范围在< 0.5到9 kΩ(平均值= 3.09,中位数= 2.00,SD = 2.15),而来自无线机器的阻抗范围在2.69到6.07 kΩ(平均值= 5.92,中位数= 5.05,SD = 2.59)。尽管在阻抗测量上存在这些差异,但两台机器测量的阻抗模式相似。无线脑电图机的阻抗测量,因此,应该是可接受的兽医临床设置。
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引用次数: 0
C-kit, flt-3, PDGFR-β, and VEGFR2 expression in canine adrenal tumors and correlation with outcome following adrenalectomy. C-kit、flt-3、PDGFR-β和VEGFR2在犬肾上腺肿瘤中的表达及其与肾上腺切除术后预后的相关性
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Kayla Harding, Carlos H De Mello Souza, Keijiro Shiomitsu, Elizabeth Maxwell, Judit Bertran

The objective of this retrospective study was to evaluate the expression of receptor tyrosine kinases (RTKs) in canine adrenal tumors and correlate this expression with features of tumor aggressiveness and survival in dogs undergoing adrenalectomy. Forty-three canine adrenal tumors were evaluated for expression of c-kit, fms-like tyrosine kinase 3 (flt-3), platelet-derived growth factor receptor-β (PDGFR-β), and vascular endothelial growth factor receptor 2 (VEGFR2) using immunohistochemistry. Tumor RTK staining characteristics were compared to normal adrenals. Medical records were reviewed for data regarding patient outcome and tumor characteristics. Expression of c-kit, flt-3, PDGFR-β, and VEGFR2 was detected in 26.9%, 92.3%, 96.2%, and 61.5% of cortical tumors and 0%, 63.2%, 47.4%, and 15.8% of pheochromocytomas, respectively. Expression of RTKs was not significantly increased when compared to normal adrenals and did not correlate with survival after adrenalectomy. Receptor tyrosine kinases are not overexpressed in canine adrenal tumors compared to normal adrenal tissue. Therapeutic inhibition of these receptors may still represent an effective approach in cases where receptor activation is present.

本回顾性研究的目的是评估受体酪氨酸激酶(RTKs)在犬肾上腺肿瘤中的表达,并将其与肾上腺切除术后犬肿瘤侵袭性和生存率的特征联系起来。采用免疫组织化学方法对43例犬肾上腺肿瘤进行c-kit、fms样酪氨酸激酶3 (flt-3)、血小板衍生生长因子受体-β (PDGFR-β)和血管内皮生长因子受体2 (VEGFR2)的表达评估。比较肿瘤与正常肾上腺的RTK染色特征。回顾了有关患者预后和肿瘤特征的医疗记录。c-kit、flt-3、PDGFR-β和VEGFR2在皮质肿瘤中的表达分别为26.9%、92.3%、96.2%和61.5%,在嗜铬细胞瘤中的表达分别为0%、63.2%、47.4%和15.8%。与正常肾上腺相比,RTKs的表达没有显著增加,并且与肾上腺切除术后的生存无关。与正常肾上腺组织相比,受体酪氨酸激酶在犬肾上腺肿瘤中不过度表达。在存在受体激活的情况下,治疗性抑制这些受体可能仍然是一种有效的方法。
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引用次数: 0
Comparison of electrocardiographic parameters in dogs with different stages of myxomatous mitral valve disease. 二尖瓣黏液瘤病不同阶段犬心电图参数的比较。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Yejin Na, Dohee Lee, Taesik Yun, Yoonhoi Koo, Yeon Chae, Hakhyun Kim, Mhan-Pyo Yang, Byeong-Teck Kang

This study evaluated changes in electrocardiographic (ECG) parameters according to the stage of myxomatous mitral valve disease (MMVD) in dogs, as well as the utility of ECG parameters as prognostic indicators for congestive heart failure (CHF). Medical records of dogs with MMVD were retrospectively searched. Dogs with MMVD (N = 101) were classified into stages B [B1 (n = 52) and B2 (n = 23)] and C (n = 26) according to the American College of Veterinary Internal Medicine guidelines. Baseline variables were collected; these included signalment, radiographic, echocardiographic, and ECG parameters. Corrected QT intervals (QTc) were calculated using the logarithmic (QTc1) and Fridericia (QTc2) formulas. The P wave duration, QTc1, and QTc2 were significantly longer in stage C than in stage B. The P wave duration cutoff of 43.5 ms had a diagnostic accuracy of 65% for differentiating CHF, with a sensitivity of 63% and a specificity of 90%. A cutoff value of 307.8 ms for QTc1 yielded a sensitivity of 62%, a specificity of 76%, and a diagnostic accuracy of 78%, and a cutoff value of 239.2 ms for QTc2 yielded a sensitivity of 62%, a specificity of 83%, and a diagnostic accuracy of 77% for diagnosing CHF. Therefore, prolonged P wave and QTc in dogs with MMVD may facilitate the prediction of CHF. Electrocardiography could provide clinicians with a readily available and cost-effective screening tool for predicting CHF, if the usefulness of ECG parameters can be verified.

本研究评估了犬黏液瘤性二尖瓣疾病(MMVD)分期的心电图(ECG)参数的变化,以及心电图参数作为充血性心力衰竭(CHF)预后指标的实用性。回顾性检索患MMVD犬的医疗记录。根据美国兽医内科学会(American College of Veterinary Internal Medicine)指南,将101只MMVD犬分为B期[B1 (N = 52)、B2期(N = 23)]和C期(N = 26)。收集基线变量;这些包括信号、x线、超声心动图和心电图参数。校正QT间期(QTc)采用对数(QTc1)和Fridericia (QTc2)公式计算。P波持续时间、QTc1和QTc2在C期明显长于b期。P波持续时间截止时间为43.5 ms,诊断CHF的准确率为65%,敏感性为63%,特异性为90%。QTc1的截止值为307.8 ms,灵敏度为62%,特异性为76%,诊断准确率为78%;QTc2的截止值为239.2 ms,诊断CHF的灵敏度为62%,特异性为83%,诊断准确率为77%。因此,MMVD犬的P波延长和QTc可能有助于预测CHF。如果心电图参数的有用性能够得到验证,心电图可以为临床医生提供一种容易获得且具有成本效益的预测CHF的筛查工具。
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引用次数: 0
Determination of the insertion position of implants for the cross-pin fixation of distal femoral physis fractures. 股骨远端骨折十字针固定植入物置入位置的确定。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Yukihiro Fujita

The cross-pin technique for the treatment of distal femoral physis fractures (specifically, Salter-Harris Type I fractures) was investigated using femurs collected from beagle cadavers. The pin was inserted from the medial surface of the femur at an inclination of approximately 30 to 45° relative to the long axis of the femur in the anteroposterior direction; the pin exit was set proximal to the origin of the long digital extensor tendon. Digital and radiographic images of the femur in the anteroposterior and lateral directions were obtained. In both types of images, the insertion angle of the pin relative to the long axis was measured. Results suggest that when inserting a pin proximal to the fracture line, the ideal position can be achieved by inclining the pin approximately 20° cranially relative to the long axis of the lateral direction of the femur, in addition to the previously described criteria.

交叉针技术用于治疗股骨远端骨折(特别是Salter-Harris I型骨折)的研究使用了从beagle尸体收集的股骨。销从股骨内侧表面插入,在前后方向上相对于股骨长轴倾斜约30至45°;钉出口位于指长伸肌腱起始点的近端。获得股骨正位和侧位的数字和x线图像。在两种类型的图像中,测量了引脚相对于长轴的插入角。结果表明,当在骨折线近端插入针时,除了前面描述的标准外,还可以通过将针相对于股骨外侧长轴向颅侧倾斜约20°来实现理想位置。
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引用次数: 0
Cardiopulmonary effects and recovery characteristics associated with 2 sedative protocols for assisted ventilation in healthy neonatal foals. 两种辅助通气镇静方案对健康新生马驹心肺效果和恢复特征的影响。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Carolyn L Kerr, Stephanie C J Keating, Luis G Arroyo, Laurent Viel

Neonatal foals may require prolonged sedation to permit ventilatory support in the first few days of life. The objective of this study was to evaluate and compare the cardiopulmonary effects and clinical recovery characteristics of 2 sedative/analgesia protocols in healthy foals receiving assisted ventilation. Foals were randomized to receive dexmedetomidine, butorphanol, and propofol (DBP) or midazolam, butorphanol, and propofol (MBP) during a 24-hour period. Infusion rates of dexmedetomidine, midazolam, and propofol were adjusted and propofol boluses administered according to set protocols to maintain optimal sedation and muscle relaxation. Ventilatory support variables were adjusted to preset targets. Physiologic variables were recorded, cardiac output (CO) measured (thermodilution), and arterial and mixed venous blood collected for gas analysis at intervals up to 24 hours. Foals in group DBP received dexmedetomidine [2.4 ± 0.5 μg/kg body weight (BW) per hour], butorphanol (13 μg/kg BW per hour), and propofol (6.97 ± 0.86 mg/kg BW per hour), whereas foals in group MBP received midazolam (0.14 ± 0.04 mg/kg BW per hour), butorphanol (13 μg/kg BW per hour), and propofol (5.98 ± 1.33 mg/kg BW per hour). Foals in the DBP group received significantly more propofol boluses (9.0 ± 3.0) than those in the MBP group (4.0 ± 2.0). Although physiologic variables remained within acceptable limits, heart rate (HR), mean arterial pressure (MAP), and cardiac index (CI) were lower in foals in the DBP group than in the MBP group. Times to sternal recumbency, standing, and nursing were significantly shorter in the DBP than MBP group. We found that MBP and DBP protocols are suitable to assist ventilatory support in neonatal foals, although MBP results in a prolonged recovery compared to DBP.

新生马驹可能需要长时间的镇静,以便在生命的最初几天进行呼吸支持。本研究的目的是评估和比较两种镇静/镇痛方案对接受辅助通气的健康马驹的心肺效果和临床恢复特征。马驹在24小时内随机接受右美托咪定、丁托啡诺和异丙酚(DBP)或咪达唑仑、丁托啡诺和异丙酚(MBP)治疗。调整右美托咪定、咪达唑仑和异丙酚的输注速率,并根据设定的方案给药异丙酚,以保持最佳的镇静和肌肉松弛。将通气支持变量调整到预设目标。记录生理变量,测量心输出量(CO)(热稀释),并每隔24小时收集动脉血和混合静脉血进行气体分析。DBP组马驹给予右美托咪定[2.4±0.5 μg/kg体重(BW) /小时]、丁托啡诺(13 μg/kg体重/小时)、异丙酚(6.97±0.86 mg/kg体重/小时),MBP组马驹给予咪达唑仑(0.14±0.04 mg/kg体重/小时)、丁托啡诺(13 μg/kg体重/小时)、异丙酚(5.98±1.33 mg/kg体重/小时)。DBP组马驹接受的异丙酚剂量(9.0±3.0)明显高于MBP组(4.0±2.0)。虽然生理变量仍在可接受范围内,但DBP组马驹的心率(HR)、平均动脉压(MAP)和心脏指数(CI)低于MBP组。DBP组到胸骨卧位、站立和护理的时间明显短于MBP组。我们发现MBP和DBP方案适合辅助新生儿马驹的呼吸支持,尽管与DBP相比,MBP会导致更长的恢复时间。
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引用次数: 0
Comparison of clinical and inflammatory parameters in dogs with pyometra before and after ovariohysterectomy. 子宫积脓犬卵巢子宫切除术前后临床及炎症参数的比较。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Soomin Ahn, Hyeona Bae, Jihu Kim, Suhee Kim, Jinho Park, Sang-Ki Kim, Dong-In Jung, DoHyeon Yu

This study aimed to identify potential biomarkers of canine pyometra and their correlations with clinical parameters. First, 90 dogs with pyometra and 26 healthy female dogs were compared. Then, paired samples (before and after ovariohysterectomy) from 22 dogs with pyometra and 9 healthy controls from the initial cohort were compared. Concentrations of acute inflammatory proteins, C-reactive protein (CRP) and serum amyloid A (SAA), and cell-free DNA (cfDNA), were significantly higher in dogs with pyometra than in clinically healthy dogs. Cell-free DNA was the most sensitive biomarker for systemic inflammation, based on the receiver operating characteristic curve analysis (area under the curve = 0.959). In addition, cfDNA and CRP were significantly associated with inflammation and organ injury-related clinical parameters. Following the surgical removal of the inflamed uterus, interleukin-6 (IL-6), high-mobility group box 1 (HMGB1), and procalcitonin (PCT) significantly decreased, whereas changes in CRP, SAA, and cfDNA were not significant. These findings indicate that cfDNA, CRP, and SAA are potential clinical biomarkers of systemic inflammation in dogs with pyometra and PCT, IL-6, and HMGB1 are potential biomarkers of clinical recovery.

本研究旨在确定犬脓脓症的潜在生物标志物及其与临床参数的相关性。首先,将90只脓膜增生犬与26只健康母犬进行比较。然后,对22只脓膜瘤犬和9只健康对照犬的配对样本(卵巢子宫切除术前后)进行比较。脓脓狗的急性炎症蛋白、c反应蛋白(CRP)、血清淀粉样蛋白A (SAA)和无细胞DNA (cfDNA)浓度显著高于临床健康狗。根据受试者工作特征曲线分析(曲线下面积= 0.959),无细胞DNA是全身性炎症最敏感的生物标志物。此外,cfDNA和CRP与炎症和器官损伤相关的临床参数显著相关。手术切除发炎子宫后,白细胞介素-6 (IL-6)、高迁移率组盒1 (HMGB1)和降钙素原(PCT)显著降低,而CRP、SAA和cfDNA的变化不显著。这些发现表明cfDNA、CRP和SAA是脓脓症犬全身炎症的潜在临床生物标志物,PCT、IL-6和HMGB1是临床恢复的潜在生物标志物。
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引用次数: 0
Conventional versus high-flow oxygen therapy in dogs with lower airway injury. 下气道损伤犬的常规氧疗与高流量氧疗比较。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Meera Ramesh, Elizabeth Thomovsky, Paula Johnson

Dogs with lower airway pathology that present in respiratory distress often receive oxygen therapy as the first line of treatment regardless of the underlying cause. Conventional "low-flow" systems deliver oxygen with a maximum flow rate of 15 L/minute. Traditionally, when an animal's respiratory status does not improve with conventional oxygen therapy and treatments for underlying disease, options might be limited to either intubation and mechanical ventilation or humane euthanasia. High-flow oxygen therapy (HFOT) has been gaining popularity in veterinary medicine as an alternative route of oxygen supplementation for animals that require support beyond conventional therapy. High-flow oxygen therapy can supply a mixture of air and oxygen via a heated and humidified circuit. It is user friendly and can be used in an environment in which mechanical ventilation is unavailable. This review article is written for emergency doctors and general practitioners who lack access to mechanical ventilation. This article briefly reviews pertinent respiratory physiology, traditional oxygen supplementation techniques, the physiology of HFOT, and the limited evidence available in veterinary medicine regarding the use of HFOT, its applications, and limitations. Guidelines for the use of HFOT are suggested and HFOT is compared to conventional therapy.

出现呼吸窘迫的下气道病理犬通常接受氧气治疗作为第一线治疗,无论其根本原因如何。传统的“低流量”系统输送氧气的最大流量为15升/分钟。传统上,当动物的呼吸状态不能通过传统的氧气治疗和潜在疾病的治疗得到改善时,选择可能仅限于插管和机械通气或人道安乐死。高流量氧疗(HFOT)在兽医学中越来越受欢迎,作为需要常规治疗之外支持的动物补充氧气的替代途径。高流量氧气疗法可以通过加热和加湿的电路提供空气和氧气的混合物。它是用户友好的,可以在没有机械通风的环境中使用。这篇综述文章是为缺乏机械通气的急诊医生和全科医生写的。本文简要综述了相关的呼吸生理学,传统的补氧技术,HFOT的生理学,以及兽医学中关于HFOT使用的有限证据,其应用和局限性。建议了HFOT的使用指南,并将HFOT与常规疗法进行了比较。
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引用次数: 0
Analytical validation of a point-of-care test and an automated immunoturbidimetric assay for the measurement of canine C-reactive protein in serum. 分析验证点护理测试和自动免疫比浊法测定血清中犬c反应蛋白。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Marshal A Covin, Robynne R Gomez, Jan S Suchodolski, Jörg M Steiner, Jonathan A Lidbury

C-reactive protein (CRP) is an acute phase protein, which is used to evaluate and monitor the response of the innate immune system to a variety of inflammatory processes in the dog. The purpose of this study was to analytically validate a point-of-care assay (IDEXX Catalyst CRP Test) and an immunoturbidimetric assay (Gentian Canine CRP Immunoassay) for the measurement of serum CRP concentrations in dogs. These 2 assays (Catalyst, Gentian) were compared to a previously validated enzyme-linked immunosorbent assay (Tridelta Development EIA Canine CRP Assay). Linearity, precision, reproducibility, and accuracy were assessed using leftover serum samples. Agreement between assays was assessed using leftover serum samples and serum from clinically healthy dogs. Observed to expected ratios (O/E) for dilutional parallelism were 83.9 to 163.1% and 108.3 to 160.6% for the Catalyst and the Gentian assays, respectively. Coefficients of variation for intra-assay variability ranged from 6.4 to 9.5% for the Catalyst assay and 1.5 to 2.6% for the Gentian assay. Coefficients of variation for inter-assay variability ranged from 3.8 to 18.2% for the Catalyst assay and 4.5 to 5.8% for the Gentian assay. The mean O/E for recovery were 97.9% and 98.5% for the Catalyst and Gentian assays, respectively. Correlations between assays were as follows: Catalyst and Tridelta (R 2 = 0.76), Gentian and Tridelta (R 2 = 0.79), and Catalyst and Gentian (R 2 = 0.98). The Catalyst and Gentian assays are both acceptable for measuring CRP in dog serum, but their results are not directly comparable with the Tridelta assay.

c反应蛋白(CRP)是一种急性期蛋白,用于评估和监测犬的先天免疫系统对各种炎症过程的反应。本研究的目的是分析验证一种即时检测法(IDEXX Catalyst CRP Test)和一种免疫比浊法(龙胆草犬CRP免疫测定法)对狗血清CRP浓度的测量。将这两种检测方法(Catalyst,龙胆草)与之前验证的酶联免疫吸附检测方法(Tridelta Development EIA犬CRP检测)进行比较。使用剩余血清样本评估线性度、精密度、重现性和准确性。使用剩余的血清样本和临床健康犬的血清来评估测定之间的一致性。观察到稀释平行度的预期比值(O/E)在Catalyst和龙胆草试验中分别为83.9 ~ 163.1%和108.3 ~ 160.6%。催化剂测定法的测定内变异系数为6.4 - 9.5%,龙胆测定法为1.5 - 2.6%。催化剂测定法的测定间变异系数为3.8 - 18.2%,龙胆草测定法为4.5 - 5.8%。催化剂和龙胆草的平均回收率分别为97.9%和98.5%。各测定指标的相关性为:Catalyst与Tridelta (r2 = 0.76)、龙胆草与Tridelta (r2 = 0.79)、Catalyst与龙胆草(r2 = 0.98)。Catalyst和龙胆草试验都可用于狗血清中CRP的测定,但其结果不能与Tridelta试验直接比较。
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引用次数: 0
Development of a biologically immortalized equine stem cell line. 生物学上永生化的马干细胞系的发展。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Rodolfo Nino-Fong, Blanca P Esparza Gonzalez, Juan Carlos Rodriguez-Lecompte, William Montelpare, Laurie McDuffee

Bone repair in horses implies invasive surgeries and increased cost. Research on musculoskeletal disorders therapy in horses includes cell-based therapy with mesenchymal stromal cells (MSCs). Mesenchymal stromal cells can be obtained from bone marrow (BMMSCs). Unfortunately, BMMSCs have limited cell replication in vitro. The objective of this study was to develop a biologically immortalized equine stem cell line derived from bone marrow, with unlimited in-vitro proliferation and the ability to differentiate into bone cells. Equine BMMSCs were transfected and immortalized with human telomerase reverse transcriptase (hTERT) gene. Cell passages from equine immortal BMMSCs were characterized by the presence of stemness CD markers and expression of multi-potent differentiation genes (OCT-4, SOX2, and NANOG). Equine immortal BMMSCs were incubated in osteogenic medium and bone cell differentiation was determined by alkaline phosphatase and von Kossa staining, and osteogenic gene expression (osteocalcin, Runx2, and osterix). Telomerase activity was determined by telomeric repeat amplification technique. Results showed that equine immortal BMMSCs were able to replicate in-vitro up to passage 50 and maintain stem cell characteristics by the presence of CD90 and expression of multi-potent genes. Equine immortal BMMSCs were able to differentiate into bone cells, which was confirmed by the positive osteogenic staining and gene expression. Equine BMMSCs were successfully immortalized and maintained characteristics of stem cells and readily differentiated into osteogenic cells. Extending the life span of equine BMMSCs by transfection of the hTERT gene will revolutionize the clinical use of MSCs by making them available to orthopedic surgeons "off the shelf."

马骨修复意味着侵入性手术和成本增加。马肌肉骨骼疾病的治疗研究包括间充质基质细胞(MSCs)的细胞治疗。间充质间质细胞可以从骨髓中获得(BMMSCs)。不幸的是,BMMSCs在体外的细胞复制有限。本研究的目的是开发一种从骨髓中提取的生物永生的马干细胞系,具有无限的体外增殖和分化成骨细胞的能力。用人端粒酶逆转录酶(hTERT)基因转染马骨髓间充质干细胞并使其永生化。来自马永生BMMSCs的细胞传代通过存在干性CD标记和多能分化基因(OCT-4, SOX2和NANOG)的表达来表征。在成骨培养基中培养马永生bmmmscs,通过碱性磷酸酶和von Kossa染色检测骨细胞分化,以及成骨基因(骨钙素、Runx2和osterix)的表达。端粒酶活性测定采用端粒重复扩增技术。结果表明,通过CD90的存在和多效基因的表达,马永生bmmmscs能够在体外复制至传代50,并保持干细胞特性。马永生骨髓间充质干细胞能够分化为骨细胞,成骨染色和基因表达阳性证实了这一点。马骨髓间充质干细胞成功永生化,保持了干细胞的特性,并容易分化为成骨细胞。通过转染hTERT基因来延长马骨髓间充质干细胞的寿命,将彻底改变骨髓间充质干细胞的临床应用,使其成为骨科医生的“现成”产品。
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引用次数: 0
Long-term in-vitro glucocorticoid treatment induces glucocorticoid resistance in canine mast cell tumors. 长期体外糖皮质激素治疗诱导犬肥大细胞肿瘤糖皮质激素耐药。
IF 1.1 4区 农林科学 Q2 Veterinary Pub Date : 2021-10-01
Akira Matsuda

Although glucocorticoid administration has produced impressive results in treating canine mast cell tumors (MCTs), in some cases, glucocorticoids fail to reduce the tumor volume, leading to tumor relapse even after treatment. To date, mechanisms involved in glucocorticoid resistance in canine MCTs remain poorly defined. The objective of this study was to establish glucocorticoid-resistant canine MCT cell lines derived from glucocorticoid-sensitive cell lines after prolonged treatment with dexamethasone (Dex). Real-time polymerase chain reaction (RT-PCR) revealed that elevation of glucocorticoid receptor (GR)-regulated gene expression was suppressed in Dex-resistant cell lines after Dex stimulation compared with parent Dex-sensitive cell lines. This indicated that GR-regulated transcription was suppressed in Dex-resistant cell lines. Insufficient expression of GRs was not detected in Dex-resistant cell lines. Possible inhibitors of GR-regulated transcription were increased in mRNA expression in Dex-resistant cell lines. In addition, it was determined that mRNA expression of drug efflux pumps and anti-apoptosis factors was higher in Dex-resistant cell lines. In conclusion, glucocorticoid-resistant canine MCT cell lines have been established that are derived from glucocorticoid-sensitive cell lines. These cell lines suggest that multiple mechanisms contribute to glucocorticoid resistance in canine MCT cells. The mechanisms of glucocorticoid resistance after long-term treatment can be further investigated using these cell lines and a novel therapeutic strategy for glucocorticoid-resistant canine MCT cells can be developed.

尽管糖皮质激素在治疗犬肥大细胞肿瘤(mct)方面取得了令人印象深刻的效果,但在某些情况下,糖皮质激素不能减少肿瘤体积,导致肿瘤在治疗后复发。迄今为止,犬mct中糖皮质激素耐药的机制仍然不明确。本研究的目的是在地塞米松(Dex)长期治疗后,从糖皮质激素敏感细胞系衍生出糖皮质激素耐药犬MCT细胞系。实时聚合酶链反应(RT-PCR)显示,与Dex敏感细胞株相比,Dex刺激后,Dex耐药细胞株糖皮质激素受体(GR)调控基因表达的升高受到抑制。这表明gr调控的转录在dex抗性细胞系中受到抑制。在dex耐药细胞系中未检测到GRs表达不足。在dex耐药细胞系中,可能的gr调控转录抑制剂的mRNA表达增加。此外,我们还发现dex耐药细胞株中药物外排泵和抗凋亡因子mRNA的表达较高。总之,糖皮质激素耐药犬MCT细胞系是由糖皮质激素敏感细胞系衍生而来。这些细胞系表明犬MCT细胞的糖皮质激素耐药有多种机制。利用这些细胞系可以进一步研究长期治疗后糖皮质激素耐药的机制,并为糖皮质激素耐药的犬MCT细胞开发新的治疗策略。
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引用次数: 0
期刊
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
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