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A Fast and Robust FTIR-ATR Coupled Chemometric Determination of Chemical and Molecular Structure of Wheat (Triticum aestivum L.) under a Series of Microbial Fermentation Processes 一种快速、稳健的FTIR-ATR联用化学计量法测定小麦(Triticum aestivum L.)在一系列微生物发酵过程中的化学和分子结构
S. Yasar, R. Tosun
This study aimed to ferment wheat grain by optimised bacteria, yeast and fungal fermentations. Crude protein, tannin, phytic acid and lactic acid contents of samples taken at 24 h intervals determined by chemical methods were compared with those of infrared (IR) spectro-chemometry. Secondary protein components were further quantified with IR spectra deconvolution method. The results indicated that some fermentations increased crude protein of wheat, whilst its tannin and phytic acid degraded in all fermentations. Wheat enriched with lactic acid content in all fermentations. FT-IR spectroscopic method accurately (99.99% of recovery) and precisely (regression coefficient of prediction R2 = 0.999, P <0.0001) predicted these nutrient contents. Fermentation positively caused a re-organised secondary protein conformation; the percentages of β-sheet and α-helix increased by 68 and 140%, respectively, whereas the random coil decreased by 63%. FT-IR spectrometry combined with suitable chemometric tools provided a fast and robust monitoring of chemical and structural changes during microbial fermentation.
本研究旨在通过优化细菌、酵母和真菌发酵来发酵小麦颗粒。将化学方法测定的样品24小时内的粗蛋白质、单宁、植酸和乳酸含量与红外光谱化学法测定的样品进行了比较。用红外光谱反褶积法对次级蛋白质组分进行进一步定量。结果表明,某些发酵过程提高了小麦的粗蛋白含量,而其单宁和植酸在所有发酵过程中都发生了降解。在所有发酵过程中富含乳酸的小麦。FT-IR光谱法准确地(99.99%的回收率)和准确地(预测回归系数R2=0.999,P<0.0001)预测了这些营养成分的含量。发酵产生重组的次级蛋白质构象;β片和α螺旋的百分比分别增加了68%和140%,而无规螺旋的百分比减少了63%。FT-IR光谱法与合适的化学计量工具相结合,可以快速、可靠地监测微生物发酵过程中的化学和结构变化。
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引用次数: 0
Interferon Regulatory Factor 3 (IRF3) Characterisation in Four Mammalian Species Using Bioinformatics Method 干扰素调节因子3 (IRF3)在四种哺乳动物中的生物信息学研究
Sanda Mofoyeke, Adenaike Adeyemi, F. Adeboye, A. Sanda, C. Ikeobi
IRF3 has gained recognition as a key transcriptional regulator of type1 interferon (IFN) gene expression which induces response to pathogenic infection. The advancement in technology and molecular biology has led to bioinformatics, this field is widely accepted because it is effective and saves time. This study made use of bioinformatics approach to identify and characterize IRF3 in four mammalian species namely: cattle, rabbit, pig and water buffalo. Evolutionary relationships inferred among the IRF3 of four mammalian and other species showed a high level of relatedness. Leucine was the most abundant residue while serine which plays a major role in maintenance of a healthy immune system was present in all the species. The information obtained in this study could serve as a guide when using the IRF3 gene as a marker to select animals for disease resistance because of the critical role they play in antiviral defense.
IRF3已被公认为1型干扰素(IFN)基因表达的关键转录调节因子,其诱导对病原性感染的反应。技术和分子生物学的进步导致了生物信息学,该领域因其有效且节省时间而被广泛接受。本研究利用生物信息学方法对牛、兔、猪和水牛四种哺乳动物的IRF3进行了鉴定和表征。在四种哺乳动物和其他物种的IRF3之间推断出的进化关系显示出高度的相关性。亮氨酸是最丰富的残基,而在维持健康免疫系统中起主要作用的丝氨酸存在于所有物种中。这项研究中获得的信息可以作为使用IRF3基因作为标记物选择抗病动物的指南,因为它们在抗病毒防御中发挥着关键作用。
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引用次数: 0
Milk Price Comparison Between Six Years from Romania and European Union 罗马尼亚与欧盟6年牛奶价格比较
A. Vâtcă, A. Rotaru, Ș. Gâdea, S. Vâtcă, Valentin Stoian
Milk price tendency is essential for the national economy and protection of milk producers. The constant need to align with European standards motivate this study to assess the interaction of milk prices in our country compared to the European Union. The objective of the study was to statistically compare the prices from Romania and UE. For a deep overview we took into consideration the quantity from Romania and most important qualitative milk parameters namely protein and fat content. The data was from 6 years from 2013 until 2018 and from all four seasons. Analysis of variance was performed together with mean comparisons between different years and seasons. Milk medium prices were higher with at least 20% in UE compared to Romania. Prices were higher in autumn and winter rather than in spring and summer proportionally with the quantity of milk. Protein and fat content was significantly higher in autumn and winter season.
牛奶价格走势对国民经济和保护牛奶生产者至关重要。与欧洲标准保持一致的持续需求促使本研究评估我国与欧盟牛奶价格的相互作用。该研究的目的是统计比较罗马尼亚和欧洲的价格。为了深入概述,我们考虑了来自罗马尼亚的数量和最重要的定性牛奶参数,即蛋白质和脂肪含量。数据来自2013年至2018年的6年,涵盖了四个季节。方差分析与不同年份和季节之间的均值比较一起进行。与罗马尼亚相比,欧洲中等牛奶价格至少高出20%。与产奶量成正比,价格在秋季和冬季高于春季和夏季。蛋白质和脂肪含量在秋冬季显著升高。
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引用次数: 0
Influence of the Housing System and the Season on Some Parameters Indicative of the Welfare of Dairy Cows in Eastern Algeria 住房制度和季节对阿尔及利亚东部奶牛福利指标的影响
I. Djaalab, H. Mansour-Djaalab, F. Riachi, A. Beghriche, S. Haffaf, Assia Allaoui, L. Ghoribi
The purpose of this study is to evaluate the effect of housing systems (free vs. tie-stall) and season (winter vs. spring) on the welfare of dairy cows through indices of cleanliness, leg health (straightness, hocks and knees), lameness score, body condition and milk production. This is to test the hypothesis that dairy cows in spring and free-stalls have a better quality of well-being than those kept in tie stalls in winter. For that, 299 dairy cows of the Holstein and Montbeliard breeds were selected from dairy farms in 6 communes of the Constantine governorate in eastern Algeria. The results showed that tie-stalled farms in winter were characterized by a lack of hygiene (P <0.001), with a stall cleanliness score of 7.62 and a frequency of 32.60% of dirty to very dirty cows. In the spring, the incidence of severe lameness does not exceed 9% in farms with tie stalls and more than 41% in mild lameness in housing with free stalls (P <0.001). Cows in tie stalls show a deviation of legs of 17° to 24° (57.97%). On the other hand, more than half of cows in free stalls show integumentary lesions (alopecia and swelling) of the carpal (60.25%) and tarsal joints (54.22%) (P <0.001). The percentage of fairly lean cows (score 2-2.5), housed in tie stalls, was significantly higher (18 to 44%) with a significantly low milk production (P <0.001). These results reflect a lack of comfort in tie-stall farms during winter which has an impact on dairy performance.
本研究的目的是通过清洁度、腿部健康(平直度、飞节和膝盖)、跛足评分、身体状况和产奶量等指标,评估住房制度(免费与搭棚)和季节(冬季与春季)对奶牛福利的影响。这是为了检验这样一种假设,即春季和自由放养的奶牛比冬季放养的奶牛有更好的健康质量。为此,从阿尔及利亚东部康斯坦丁省6个公社的奶牛场中挑选了299头荷斯坦和蒙贝利亚品种的奶牛。结果表明,冬季扎堆养殖场的特点是缺乏卫生(P<0.001),牛棚清洁度得分为7.62,脏奶牛到非常脏奶牛的频率为32.60%。春季,在有扎棚的农场中,严重跛脚的发生率不超过9%,在有自由棚的住房中,轻度跛脚的发病率不超过41%(P<0.001)。扎棚中的奶牛腿的偏差为17°至24°(57.97%)。另一方面,超过一半的自由饲养的奶牛表现出腕关节(60.25%)和跗骨关节(54.22%)的表皮损伤(脱发和肿胀)(P<0.001),显著较高(18%至44%),而牛奶产量显著较低(P<0.001)。这些结果反映了冬季扎堆农场缺乏舒适感,这对奶牛的性能产生了影响。
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引用次数: 0
Ghrelin (GHRL) Gene Polymorphism and its Association with Growth and Body Size Parameters in Three Nigerian Chicken Breeds 3个尼日利亚鸡品种生长素(GHRL)基因多态性及其与生长和体型参数的关系
A. Sanda, M. Bemji, M. Wheto, A. Oso, M. O. Sanda, O. Olowofeso
This study was conducted to identify ghrelin (GHRL) gene polymorphism within exons 2 and 3 in three chicken breeds and to determine its association with growth and linear body measurements. Three hundred one-day-old chicks comprising 100 each of three chicken breeds (FUNAAB Alpha, Noiler and Shika Brown) were used for the research and were raised for eighteen weeks. Fifty birds per breed were sampled for blood collection and Genomic DNA was extracted using Zymo miniprep kit. DNA was amplified and PCR products digested with Eco72I restriction enzyme. Growth data were analysed using Generalized Linear Model of SAS. Noiler had the highest (P <0.05) growth traits from 10 to 18 weeks among studied breeds. GHRL gene polymorphism had no significant (P >0.05) effect on growth traits. However, interaction between GHRL gene polymorphism and chicken breeds revealed that Noiler chickens AA and AB had the best (P <0.05) productive performance from 2 to 18 weeks.
本研究旨在鉴定三种鸡品种的ghrelin (GHRL)基因外显子2和3的多态性,并确定其与生长和线性体测量的关系。研究使用了300只1日龄的雏鸡,其中3个品种(FUNAAB Alpha、Noiler和Shika Brown)各100只,饲养18周。每个品种50只采集血样,使用Zymo迷你试剂盒提取基因组DNA。DNA扩增,PCR产物用Eco72I酶切。生长数据采用SAS广义线性模型进行分析。黑鸡对生长性状影响最大(p0.05)。然而,GHRL基因多态性与鸡品种的互作表明,AA和AB在2 ~ 18周龄的生产性能最好(P <0.05)。
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引用次数: 0
Effects of Saccharomyces cerevisiae Addition in Dairy Cows Diets 奶牛日粮中添加酿酒酵母的效果
M. Enculescu
The aim of this study was to evaluate the effects of fresh yeast (Saccharomyces cerevisiae) supplementation in the dairy cows’ diets on productive performances and health status. The study was carried out in the experimental farm of the Research and Development Institute for Bovine Balotești on 50 multiparous Romanian Black and Spotted dairy cows, randomly divided into two groups (N = 25 heads/group), according to age, milk yield, body weight and health status. The experimental group received 80 g Saccharomyces cerevisiae/head/day for one year. The groups were fed with the same diet and had free access to water and salt. Results were expressed as a mean (±Standard Deviation). The t-test was applied to obtain the significance of difference. Supplementation of the diet with Saccharomyces cerevisiae had a significant effect (P <0.001) on milk yield (20.71±1.65 l/head/day) for the experimental group comparing with the control group (18.22±1.81 l/head/day), and on milk protein and lactose (P <0.05). The addition of Saccharomyces cerevisiae in dairy cows’ diet did not improve the milk fat, hematological and biochemical/urine indicators in the experimental group (P >0.05). However, for alkaline phosphatase, differences at the end of the study have been observed (P <0.01). The beneficial effect of the yeast and yeast products in ruminants could be attributed to microbial activity by increasing the number of beneficial bacteria in the rumen of the animals. The use of Saccharomyces cerevisiae as an alternative source of economic protein, vitamins and minerals in dairy cows’ diet represents an effective measure to optimize animal productivity.
本研究的目的是评估奶牛日粮中添加新鲜酵母(酿酒酵母)对生产性能和健康状况的影响。这项研究是在Balotești牛研究开发所的实验农场对50头多胎罗马尼亚黑斑奶牛进行的,根据年龄、产奶量、体重和健康状况随机分为两组(N=25头/组)。实验组接受80克酿酒酵母/头/天,为期一年。这两组人的饮食相同,可以免费获得水和盐。结果以平均值(±标准差)表示。应用t检验来获得差异的显著性。日粮中添加酿酒酵母有显著效果(P 0.05)。然而,对于碱性磷酸酶,在研究结束时观察到差异(P<0.01)。酵母和酵母制品对反刍动物的有益作用可以归因于微生物活性,增加了动物瘤胃中的有益细菌数量。酿酒酵母作为奶牛饮食中经济蛋白质、维生素和矿物质的替代来源,是优化动物生产力的有效措施。
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引用次数: 0
Training of Ecological Detection Dogs for Wolf Scat (Canis lupus) 狼毒(犬狼疮)生态检测犬的训练
H. Vervaecke, E. Krunkelsven, K. V. D. Berge
Detection dogs for wolf scat can assist in conservation-monitoring of wolves. In this pilot project, the first encounters of experienced detection dogs with wolf scat were documented. It was tested how a dog, trained with only a few scat samples (minimally four, maximally six), could generalise towards new unknown wolf scats and discriminate them from distracting odours. Four dogs all showed a behavioural expression of aversion upon first presentation with wolf scat during their initial training to fixate on wolf scat. The dog that showed the least aversion to wolf scats was trained during two weeks. His ability to discriminate wolf scat from other odours and to generalise to new wolf samples was evaluated. He was presented a line-up with new wolf scat, dog scat and other distracting odours in eight boxes, seven trials and random rotations of odours. The test was conducted blindly without the dog handler knowing if or where a positive sample was present. The dog showed partial generalisation of wolf scat odour from only a few known training samples to new wolf samples. The test was evaluated for methodological improvements.
狼粪探测犬可以帮助对狼进行保护监测。在这个试点项目中,记录了经验丰富的侦查犬与狼粪的首次相遇。研究人员测试了一只只接受少量粪便样本(最少4种,最多6种)训练的狗如何识别未知的狼粪便,并将它们与分散注意力的气味区分开来。在最初的狼粪注视训练中,4只狗在第一次看到狼粪时都表现出厌恶的行为表达。对狼粪表现出最少厌恶的狗接受了两周的训练。他的能力区分狼粪和其他气味,并推广到新的狼样本进行了评估。他在8个盒子里放了一组新的狼屎、狗屎和其他分散注意力的气味,进行了7次试验,并随机轮换了气味。测试是盲目进行的,训犬员不知道是否或在哪里存在阳性样本。从少数已知的训练样本到新的狼样本,狗表现出对狼粪气味的部分概括。对该试验进行了评价,以改进方法。
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引用次数: 0
Enrofloxacin and Ciprofloxacin Residues in Table Eggs: Distribution and Heat Treatment Effect 餐蛋中恩诺沙星和环丙沙星的残留分布及热处理效果
A. Alaboudi, D. Almashhadany, E. Abu-Basha, I. Musallam
A 500 composite egg samples (2500 eggs-each sample represented by 5 eggs) collected from layer farms and local markets from all over Jordan were studied for presence of antimicrobials using Premi®Test screening test. Positive samples indicated by inhibition of microbial growth represented 12.8% out of total screened egg samples. Positive samples were examined quantitatively using HPLC technique to detect the presence of enrofloxacin and ciprofloxacin. Ciprofloxacin and enrofloxacin residues were detected in 1% and 0.8% of the total samples, respectively, where both drugs were recovered from white but not yolk. The effect of boiling on either drugs concentration in fortified white or yolk was demonstrated by gradual increases in the mean reduction percentages within treatment time with an average of 87% reduction after 15 minutes of boiling for both drugs and egg compartments and 5 minutes of frying at 160°C. The average concentration reduction percentages by the end of four weeks of refrigeration of fortified yolk and white were around 45 and 50% for enrofloxacin and ciprofloxacin respectively. The significance and mechanism of drug deposition and reduction during processing is being highlighted.
采用Premi®Test筛选试验,研究了从约旦各地的蛋场和当地市场收集的500个复合鸡蛋样本(2500个鸡蛋,每个样本代表5个鸡蛋)是否存在抗菌药物。微生物生长抑制阳性样品占总筛选鸡蛋样品的12.8%。采用高效液相色谱法定量检测阳性样品中恩诺沙星和环丙沙星的存在。环丙沙星和恩诺沙星的残留量分别占总样品的1%和0.8%,其中两种药物均从蛋白中回收,而不是从蛋黄中回收。煮沸对强化蛋白或蛋黄中药物浓度的影响可以通过处理时间内平均还原百分比的逐渐增加来证明,在药物和鸡蛋区煮沸15分钟和在160°C下煎炸5分钟后,平均还原率为87%。强化蛋黄和蛋清冷藏4周后,恩诺沙星和环丙沙星的平均浓度降低率分别约为45%和50%。药物在加工过程中沉积和还原的意义和机制正在得到强调。
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引用次数: 1
Bivalve Mariculture in Two – Way Interaction with Phytoplankton: A Review of Feeding Mechanism and Nutrient Recycling 与浮游植物双向相互作用的双瓣海水养殖:摄食机制和养分循环研究综述
R. Moruf, G. F. Okunade, O. W. Elegbeleye
Bivalve mariculture is a type of molluscan farming done in open seawater on racks, rafts or longlines where naturally occurring phytoplankton serves as a key food item, introduced into the enclosures with the normal circulation of seawater. Increasingly, the reverse trophic interaction is being recognized; dissolved inorganic and organic waste compounds released by metabolically active bivalves can supply phytoplankton with nutrient and energy requirements for their growth. This two-way interaction can be viewed as a type of community symbiosis developed over long evolutionary timescales. The extent to which this affects overall nutrient budgets and thus primary production is related to the system flushing rate and residence time. Here we reviewed the feeding mechanism and nutrient recycling activities of bivalve and also emphasized the role of phytoplankton as a key nutritional live feed in sustainable bivalve mariculture. Bivalves influence nutrient dynamics through direct excretion and indirectly through microbial mediated remineralisation of their organic deposits in the sediments. The quantitative knowledge of bivalve phytoplankton trophic interactions in coastal waters will inform bivalve mariculture development to effectively serve the needs of both seafood production and ecosystem restoration.
双壳类海水养殖是一种在开放海水中进行的软体动物养殖,在架子、筏子或延绳钓上进行,其中自然产生的浮游植物作为主要食物,随着海水的正常循环被引入围场。越来越多的人认识到反向营养相互作用;代谢活跃的双壳类释放的溶解的无机和有机废物化合物可以为浮游植物提供生长所需的营养和能量。这种双向互动可以被视为一种长期进化时间尺度上发展起来的群落共生。这在多大程度上影响了总体营养预算,从而影响了初级生产,这与系统冲洗速度和停留时间有关。本文综述了双壳类的取食机理和营养循环活动,并强调了浮游植物作为双壳类可持续海水养殖中重要的营养活饲料的作用。双壳类通过直接排泄和间接通过微生物介导的沉积物中有机沉积物的再矿化影响营养动态。对沿海水域双壳类浮游植物营养相互作用的定量认识将为双壳类海水养殖的发展提供信息,以有效地满足海产品生产和生态系统恢复的需要。
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引用次数: 4
Antibody Response and Haematological Changes of Nigerian Locally Adapted Turkeys to Sheep Red Blood Cells (SRBC) 尼日利亚地方适应火鸡对绵羊红细胞的抗体反应和血液学变化
A. Ogundero, M. O. Sanda, A. Adenaike, M. Takeet, C. Ikeobi
Haemagglutination assay and haematological analysis of 143 poults generated as F1 individuals by artificial insemination from randomly selected turkeys of White, Black and Lavender genotypes which are classified by antibody titre was carried out so as to confirm their antibody titre levels in response to sheep red blood cells (SRBC). Results showed that mean values obtained for high and low antibody titres were 7.31 and 2.67 respectively, resulting in the classification of the turkeys into Black high and low, Lavender high and black, and White high and low antibody titres. The genotype’s titre had significant (P <0.05) effect on the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) and basophil (BAS) of the basal haematological parameters. Genotype’s titre had no significant (P >0.05) effect on the primary response haematological parameters. Meanwhile, the primary response haematological parameters to SRBC antigen varied along the genotypes with the WBC increasing drastically in all the genotypes, signifying the presence of an antigen. The study concluded that the F1 turkey poult population studied diverged along the high and low antibody titre in response to SRBC. Thus, the F1 generation of the high antibody titre genotypes (Black high, White high and Lavender high) can be used as foundation stock for selection of local turkeys for high antibody titre.
对随机选取按抗体滴度分类的白、黑、薰衣草基因型火鸡进行人工授精繁殖的F1雏鸡143只进行了血凝试验和血液学分析,以确定它们对绵羊红细胞(SRBC)的抗体滴度。结果表明,高抗体滴度和低抗体滴度的平均值分别为7.31和2.67,从而将火鸡分为黑色高抗体滴度和低抗体滴度,薰衣草高抗体滴度和黑色抗体滴度,白色高抗体滴度和低抗体滴度。基因型滴度对主要反应血液学参数有显著影响(p0.05)。同时,对SRBC抗原的主要反应血液学参数沿基因型变化,WBC在所有基因型中均急剧增加,表明抗原的存在。该研究得出结论,F1火鸡种群在对SRBC的反应中沿高抗体滴度和低抗体滴度分化。因此,高抗体滴度基因型(黑高、白高和薰衣草高)的F1代可作为选择高抗体滴度地方火鸡的基础原料。
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引用次数: 0
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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