Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2020.0016
S. Yasar, R. Tosun
This study aimed to ferment wheat grain by optimised bacteria, yeast and fungal fermentations. Crude protein, tannin, phytic acid and lactic acid contents of samples taken at 24 h intervals determined by chemical methods were compared with those of infrared (IR) spectro-chemometry. Secondary protein components were further quantified with IR spectra deconvolution method. The results indicated that some fermentations increased crude protein of wheat, whilst its tannin and phytic acid degraded in all fermentations. Wheat enriched with lactic acid content in all fermentations. FT-IR spectroscopic method accurately (99.99% of recovery) and precisely (regression coefficient of prediction R2 = 0.999, P <0.0001) predicted these nutrient contents. Fermentation positively caused a re-organised secondary protein conformation; the percentages of β-sheet and α-helix increased by 68 and 140%, respectively, whereas the random coil decreased by 63%. FT-IR spectrometry combined with suitable chemometric tools provided a fast and robust monitoring of chemical and structural changes during microbial fermentation.
{"title":"A Fast and Robust FTIR-ATR Coupled Chemometric Determination of Chemical and Molecular Structure of Wheat (Triticum aestivum L.) under a Series of Microbial Fermentation Processes","authors":"S. Yasar, R. Tosun","doi":"10.15835/BUASVMCN-ASB:2020.0016","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2020.0016","url":null,"abstract":"This study aimed to ferment wheat grain by optimised bacteria, yeast and fungal fermentations. Crude protein, tannin, phytic acid and lactic acid contents of samples taken at 24 h intervals determined by chemical methods were compared with those of infrared (IR) spectro-chemometry. Secondary protein components were further quantified with IR spectra deconvolution method. The results indicated that some fermentations increased crude protein of wheat, whilst its tannin and phytic acid degraded in all fermentations. Wheat enriched with lactic acid content in all fermentations. FT-IR spectroscopic method accurately (99.99% of recovery) and precisely (regression coefficient of prediction R2 = 0.999, P <0.0001) predicted these nutrient contents. Fermentation positively caused a re-organised secondary protein conformation; the percentages of β-sheet and α-helix increased by 68 and 140%, respectively, whereas the random coil decreased by 63%. FT-IR spectrometry combined with suitable chemometric tools provided a fast and robust monitoring of chemical and structural changes during microbial fermentation.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49601533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2020.0013
Sanda Mofoyeke, Adenaike Adeyemi, F. Adeboye, A. Sanda, C. Ikeobi
IRF3 has gained recognition as a key transcriptional regulator of type1 interferon (IFN) gene expression which induces response to pathogenic infection. The advancement in technology and molecular biology has led to bioinformatics, this field is widely accepted because it is effective and saves time. This study made use of bioinformatics approach to identify and characterize IRF3 in four mammalian species namely: cattle, rabbit, pig and water buffalo. Evolutionary relationships inferred among the IRF3 of four mammalian and other species showed a high level of relatedness. Leucine was the most abundant residue while serine which plays a major role in maintenance of a healthy immune system was present in all the species. The information obtained in this study could serve as a guide when using the IRF3 gene as a marker to select animals for disease resistance because of the critical role they play in antiviral defense.
{"title":"Interferon Regulatory Factor 3 (IRF3) Characterisation in Four Mammalian Species Using Bioinformatics Method","authors":"Sanda Mofoyeke, Adenaike Adeyemi, F. Adeboye, A. Sanda, C. Ikeobi","doi":"10.15835/BUASVMCN-ASB:2020.0013","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2020.0013","url":null,"abstract":"IRF3 has gained recognition as a key transcriptional regulator of type1 interferon (IFN) gene expression which induces response to pathogenic infection. The advancement in technology and molecular biology has led to bioinformatics, this field is widely accepted because it is effective and saves time. This study made use of bioinformatics approach to identify and characterize IRF3 in four mammalian species namely: cattle, rabbit, pig and water buffalo. Evolutionary relationships inferred among the IRF3 of four mammalian and other species showed a high level of relatedness. Leucine was the most abundant residue while serine which plays a major role in maintenance of a healthy immune system was present in all the species. The information obtained in this study could serve as a guide when using the IRF3 gene as a marker to select animals for disease resistance because of the critical role they play in antiviral defense.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46704528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2021.0003
A. Vâtcă, A. Rotaru, Ș. Gâdea, S. Vâtcă, Valentin Stoian
Milk price tendency is essential for the national economy and protection of milk producers. The constant need to align with European standards motivate this study to assess the interaction of milk prices in our country compared to the European Union. The objective of the study was to statistically compare the prices from Romania and UE. For a deep overview we took into consideration the quantity from Romania and most important qualitative milk parameters namely protein and fat content. The data was from 6 years from 2013 until 2018 and from all four seasons. Analysis of variance was performed together with mean comparisons between different years and seasons. Milk medium prices were higher with at least 20% in UE compared to Romania. Prices were higher in autumn and winter rather than in spring and summer proportionally with the quantity of milk. Protein and fat content was significantly higher in autumn and winter season.
{"title":"Milk Price Comparison Between Six Years from Romania and European Union","authors":"A. Vâtcă, A. Rotaru, Ș. Gâdea, S. Vâtcă, Valentin Stoian","doi":"10.15835/BUASVMCN-ASB:2021.0003","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2021.0003","url":null,"abstract":"Milk price tendency is essential for the national economy and protection of milk producers. The constant need to align with European standards motivate this study to assess the interaction of milk prices in our country compared to the European Union. The objective of the study was to statistically compare the prices from Romania and UE. For a deep overview we took into consideration the quantity from Romania and most important qualitative milk parameters namely protein and fat content. The data was from 6 years from 2013 until 2018 and from all four seasons. Analysis of variance was performed together with mean comparisons between different years and seasons. Milk medium prices were higher with at least 20% in UE compared to Romania. Prices were higher in autumn and winter rather than in spring and summer proportionally with the quantity of milk. Protein and fat content was significantly higher in autumn and winter season.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47428273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2020.0020
I. Djaalab, H. Mansour-Djaalab, F. Riachi, A. Beghriche, S. Haffaf, Assia Allaoui, L. Ghoribi
The purpose of this study is to evaluate the effect of housing systems (free vs. tie-stall) and season (winter vs. spring) on the welfare of dairy cows through indices of cleanliness, leg health (straightness, hocks and knees), lameness score, body condition and milk production. This is to test the hypothesis that dairy cows in spring and free-stalls have a better quality of well-being than those kept in tie stalls in winter. For that, 299 dairy cows of the Holstein and Montbeliard breeds were selected from dairy farms in 6 communes of the Constantine governorate in eastern Algeria. The results showed that tie-stalled farms in winter were characterized by a lack of hygiene (P <0.001), with a stall cleanliness score of 7.62 and a frequency of 32.60% of dirty to very dirty cows. In the spring, the incidence of severe lameness does not exceed 9% in farms with tie stalls and more than 41% in mild lameness in housing with free stalls (P <0.001). Cows in tie stalls show a deviation of legs of 17° to 24° (57.97%). On the other hand, more than half of cows in free stalls show integumentary lesions (alopecia and swelling) of the carpal (60.25%) and tarsal joints (54.22%) (P <0.001). The percentage of fairly lean cows (score 2-2.5), housed in tie stalls, was significantly higher (18 to 44%) with a significantly low milk production (P <0.001). These results reflect a lack of comfort in tie-stall farms during winter which has an impact on dairy performance.
{"title":"Influence of the Housing System and the Season on Some Parameters Indicative of the Welfare of Dairy Cows in Eastern Algeria","authors":"I. Djaalab, H. Mansour-Djaalab, F. Riachi, A. Beghriche, S. Haffaf, Assia Allaoui, L. Ghoribi","doi":"10.15835/BUASVMCN-ASB:2020.0020","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2020.0020","url":null,"abstract":"The purpose of this study is to evaluate the effect of housing systems (free vs. tie-stall) and season (winter vs. spring) on the welfare of dairy cows through indices of cleanliness, leg health (straightness, hocks and knees), lameness score, body condition and milk production. This is to test the hypothesis that dairy cows in spring and free-stalls have a better quality of well-being than those kept in tie stalls in winter. For that, 299 dairy cows of the Holstein and Montbeliard breeds were selected from dairy farms in 6 communes of the Constantine governorate in eastern Algeria. The results showed that tie-stalled farms in winter were characterized by a lack of hygiene (P <0.001), with a stall cleanliness score of 7.62 and a frequency of 32.60% of dirty to very dirty cows. In the spring, the incidence of severe lameness does not exceed 9% in farms with tie stalls and more than 41% in mild lameness in housing with free stalls (P <0.001). Cows in tie stalls show a deviation of legs of 17° to 24° (57.97%). On the other hand, more than half of cows in free stalls show integumentary lesions (alopecia and swelling) of the carpal (60.25%) and tarsal joints (54.22%) (P <0.001). The percentage of fairly lean cows (score 2-2.5), housed in tie stalls, was significantly higher (18 to 44%) with a significantly low milk production (P <0.001). These results reflect a lack of comfort in tie-stall farms during winter which has an impact on dairy performance.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41520613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2020.0019
A. Sanda, M. Bemji, M. Wheto, A. Oso, M. O. Sanda, O. Olowofeso
This study was conducted to identify ghrelin (GHRL) gene polymorphism within exons 2 and 3 in three chicken breeds and to determine its association with growth and linear body measurements. Three hundred one-day-old chicks comprising 100 each of three chicken breeds (FUNAAB Alpha, Noiler and Shika Brown) were used for the research and were raised for eighteen weeks. Fifty birds per breed were sampled for blood collection and Genomic DNA was extracted using Zymo miniprep kit. DNA was amplified and PCR products digested with Eco72I restriction enzyme. Growth data were analysed using Generalized Linear Model of SAS. Noiler had the highest (P <0.05) growth traits from 10 to 18 weeks among studied breeds. GHRL gene polymorphism had no significant (P >0.05) effect on growth traits. However, interaction between GHRL gene polymorphism and chicken breeds revealed that Noiler chickens AA and AB had the best (P <0.05) productive performance from 2 to 18 weeks.
{"title":"Ghrelin (GHRL) Gene Polymorphism and its Association with Growth and Body Size Parameters in Three Nigerian Chicken Breeds","authors":"A. Sanda, M. Bemji, M. Wheto, A. Oso, M. O. Sanda, O. Olowofeso","doi":"10.15835/BUASVMCN-ASB:2020.0019","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2020.0019","url":null,"abstract":"This study was conducted to identify ghrelin (GHRL) gene polymorphism within exons 2 and 3 in three chicken breeds and to determine its association with growth and linear body measurements. Three hundred one-day-old chicks comprising 100 each of three chicken breeds (FUNAAB Alpha, Noiler and Shika Brown) were used for the research and were raised for eighteen weeks. Fifty birds per breed were sampled for blood collection and Genomic DNA was extracted using Zymo miniprep kit. DNA was amplified and PCR products digested with Eco72I restriction enzyme. Growth data were analysed using Generalized Linear Model of SAS. Noiler had the highest (P <0.05) growth traits from 10 to 18 weeks among studied breeds. GHRL gene polymorphism had no significant (P >0.05) effect on growth traits. However, interaction between GHRL gene polymorphism and chicken breeds revealed that Noiler chickens AA and AB had the best (P <0.05) productive performance from 2 to 18 weeks.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44418158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2020.0022
M. Enculescu
The aim of this study was to evaluate the effects of fresh yeast (Saccharomyces cerevisiae) supplementation in the dairy cows’ diets on productive performances and health status. The study was carried out in the experimental farm of the Research and Development Institute for Bovine Balotești on 50 multiparous Romanian Black and Spotted dairy cows, randomly divided into two groups (N = 25 heads/group), according to age, milk yield, body weight and health status. The experimental group received 80 g Saccharomyces cerevisiae/head/day for one year. The groups were fed with the same diet and had free access to water and salt. Results were expressed as a mean (±Standard Deviation). The t-test was applied to obtain the significance of difference. Supplementation of the diet with Saccharomyces cerevisiae had a significant effect (P <0.001) on milk yield (20.71±1.65 l/head/day) for the experimental group comparing with the control group (18.22±1.81 l/head/day), and on milk protein and lactose (P <0.05). The addition of Saccharomyces cerevisiae in dairy cows’ diet did not improve the milk fat, hematological and biochemical/urine indicators in the experimental group (P >0.05). However, for alkaline phosphatase, differences at the end of the study have been observed (P <0.01). The beneficial effect of the yeast and yeast products in ruminants could be attributed to microbial activity by increasing the number of beneficial bacteria in the rumen of the animals. The use of Saccharomyces cerevisiae as an alternative source of economic protein, vitamins and minerals in dairy cows’ diet represents an effective measure to optimize animal productivity.
{"title":"Effects of Saccharomyces cerevisiae Addition in Dairy Cows Diets","authors":"M. Enculescu","doi":"10.15835/BUASVMCN-ASB:2020.0022","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2020.0022","url":null,"abstract":"The aim of this study was to evaluate the effects of fresh yeast (Saccharomyces cerevisiae) supplementation in the dairy cows’ diets on productive performances and health status. The study was carried out in the experimental farm of the Research and Development Institute for Bovine Balotești on 50 multiparous Romanian Black and Spotted dairy cows, randomly divided into two groups (N = 25 heads/group), according to age, milk yield, body weight and health status. The experimental group received 80 g Saccharomyces cerevisiae/head/day for one year. The groups were fed with the same diet and had free access to water and salt. Results were expressed as a mean (±Standard Deviation). The t-test was applied to obtain the significance of difference. Supplementation of the diet with Saccharomyces cerevisiae had a significant effect (P <0.001) on milk yield (20.71±1.65 l/head/day) for the experimental group comparing with the control group (18.22±1.81 l/head/day), and on milk protein and lactose (P <0.05). The addition of Saccharomyces cerevisiae in dairy cows’ diet did not improve the milk fat, hematological and biochemical/urine indicators in the experimental group (P >0.05). However, for alkaline phosphatase, differences at the end of the study have been observed (P <0.01). The beneficial effect of the yeast and yeast products in ruminants could be attributed to microbial activity by increasing the number of beneficial bacteria in the rumen of the animals. The use of Saccharomyces cerevisiae as an alternative source of economic protein, vitamins and minerals in dairy cows’ diet represents an effective measure to optimize animal productivity.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47432423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2020.0021
H. Vervaecke, E. Krunkelsven, K. V. D. Berge
Detection dogs for wolf scat can assist in conservation-monitoring of wolves. In this pilot project, the first encounters of experienced detection dogs with wolf scat were documented. It was tested how a dog, trained with only a few scat samples (minimally four, maximally six), could generalise towards new unknown wolf scats and discriminate them from distracting odours. Four dogs all showed a behavioural expression of aversion upon first presentation with wolf scat during their initial training to fixate on wolf scat. The dog that showed the least aversion to wolf scats was trained during two weeks. His ability to discriminate wolf scat from other odours and to generalise to new wolf samples was evaluated. He was presented a line-up with new wolf scat, dog scat and other distracting odours in eight boxes, seven trials and random rotations of odours. The test was conducted blindly without the dog handler knowing if or where a positive sample was present. The dog showed partial generalisation of wolf scat odour from only a few known training samples to new wolf samples. The test was evaluated for methodological improvements.
{"title":"Training of Ecological Detection Dogs for Wolf Scat (Canis lupus)","authors":"H. Vervaecke, E. Krunkelsven, K. V. D. Berge","doi":"10.15835/BUASVMCN-ASB:2020.0021","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2020.0021","url":null,"abstract":"Detection dogs for wolf scat can assist in conservation-monitoring of wolves. In this pilot project, the first encounters of experienced detection dogs with wolf scat were documented. It was tested how a dog, trained with only a few scat samples (minimally four, maximally six), could generalise towards new unknown wolf scats and discriminate them from distracting odours. Four dogs all showed a behavioural expression of aversion upon first presentation with wolf scat during their initial training to fixate on wolf scat. The dog that showed the least aversion to wolf scats was trained during two weeks. His ability to discriminate wolf scat from other odours and to generalise to new wolf samples was evaluated. He was presented a line-up with new wolf scat, dog scat and other distracting odours in eight boxes, seven trials and random rotations of odours. The test was conducted blindly without the dog handler knowing if or where a positive sample was present. The dog showed partial generalisation of wolf scat odour from only a few known training samples to new wolf samples. The test was evaluated for methodological improvements.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47466748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-ASB:2020.0024
A. Alaboudi, D. Almashhadany, E. Abu-Basha, I. Musallam
A 500 composite egg samples (2500 eggs-each sample represented by 5 eggs) collected from layer farms and local markets from all over Jordan were studied for presence of antimicrobials using Premi®Test screening test. Positive samples indicated by inhibition of microbial growth represented 12.8% out of total screened egg samples. Positive samples were examined quantitatively using HPLC technique to detect the presence of enrofloxacin and ciprofloxacin. Ciprofloxacin and enrofloxacin residues were detected in 1% and 0.8% of the total samples, respectively, where both drugs were recovered from white but not yolk. The effect of boiling on either drugs concentration in fortified white or yolk was demonstrated by gradual increases in the mean reduction percentages within treatment time with an average of 87% reduction after 15 minutes of boiling for both drugs and egg compartments and 5 minutes of frying at 160°C. The average concentration reduction percentages by the end of four weeks of refrigeration of fortified yolk and white were around 45 and 50% for enrofloxacin and ciprofloxacin respectively. The significance and mechanism of drug deposition and reduction during processing is being highlighted.
{"title":"Enrofloxacin and Ciprofloxacin Residues in Table Eggs: Distribution and Heat Treatment Effect","authors":"A. Alaboudi, D. Almashhadany, E. Abu-Basha, I. Musallam","doi":"10.15835/BUASVMCN-ASB:2020.0024","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:2020.0024","url":null,"abstract":"A 500 composite egg samples (2500 eggs-each sample represented by 5 eggs) collected from layer farms and local markets from all over Jordan were studied for presence of antimicrobials using Premi®Test screening test. Positive samples indicated by inhibition of microbial growth represented 12.8% out of total screened egg samples. Positive samples were examined quantitatively using HPLC technique to detect the presence of enrofloxacin and ciprofloxacin. Ciprofloxacin and enrofloxacin residues were detected in 1% and 0.8% of the total samples, respectively, where both drugs were recovered from white but not yolk. The effect of boiling on either drugs concentration in fortified white or yolk was demonstrated by gradual increases in the mean reduction percentages within treatment time with an average of 87% reduction after 15 minutes of boiling for both drugs and egg compartments and 5 minutes of frying at 160°C. The average concentration reduction percentages by the end of four weeks of refrigeration of fortified yolk and white were around 45 and 50% for enrofloxacin and ciprofloxacin respectively. The significance and mechanism of drug deposition and reduction during processing is being highlighted.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45929618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-18DOI: 10.15835/buasvmcn-asb:2020.0010
R. Moruf, G. F. Okunade, O. W. Elegbeleye
Bivalve mariculture is a type of molluscan farming done in open seawater on racks, rafts or longlines where naturally occurring phytoplankton serves as a key food item, introduced into the enclosures with the normal circulation of seawater. Increasingly, the reverse trophic interaction is being recognized; dissolved inorganic and organic waste compounds released by metabolically active bivalves can supply phytoplankton with nutrient and energy requirements for their growth. This two-way interaction can be viewed as a type of community symbiosis developed over long evolutionary timescales. The extent to which this affects overall nutrient budgets and thus primary production is related to the system flushing rate and residence time. Here we reviewed the feeding mechanism and nutrient recycling activities of bivalve and also emphasized the role of phytoplankton as a key nutritional live feed in sustainable bivalve mariculture. Bivalves influence nutrient dynamics through direct excretion and indirectly through microbial mediated remineralisation of their organic deposits in the sediments. The quantitative knowledge of bivalve phytoplankton trophic interactions in coastal waters will inform bivalve mariculture development to effectively serve the needs of both seafood production and ecosystem restoration.
{"title":"Bivalve Mariculture in Two – Way Interaction with Phytoplankton: A Review of Feeding Mechanism and Nutrient Recycling","authors":"R. Moruf, G. F. Okunade, O. W. Elegbeleye","doi":"10.15835/buasvmcn-asb:2020.0010","DOIUrl":"https://doi.org/10.15835/buasvmcn-asb:2020.0010","url":null,"abstract":"Bivalve mariculture is a type of molluscan farming done in open seawater on racks, rafts or longlines where naturally occurring phytoplankton serves as a key food item, introduced into the enclosures with the normal circulation of seawater. Increasingly, the reverse trophic interaction is being recognized; dissolved inorganic and organic waste compounds released by metabolically active bivalves can supply phytoplankton with nutrient and energy requirements for their growth. This two-way interaction can be viewed as a type of community symbiosis developed over long evolutionary timescales. The extent to which this affects overall nutrient budgets and thus primary production is related to the system flushing rate and residence time. Here we reviewed the feeding mechanism and nutrient recycling activities of bivalve and also emphasized the role of phytoplankton as a key nutritional live feed in sustainable bivalve mariculture. Bivalves influence nutrient dynamics through direct excretion and indirectly through microbial mediated remineralisation of their organic deposits in the sediments. The quantitative knowledge of bivalve phytoplankton trophic interactions in coastal waters will inform bivalve mariculture development to effectively serve the needs of both seafood production and ecosystem restoration.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41505178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-18DOI: 10.15835/buasvmcn-asb:2020.0005
A. Ogundero, M. O. Sanda, A. Adenaike, M. Takeet, C. Ikeobi
Haemagglutination assay and haematological analysis of 143 poults generated as F1 individuals by artificial insemination from randomly selected turkeys of White, Black and Lavender genotypes which are classified by antibody titre was carried out so as to confirm their antibody titre levels in response to sheep red blood cells (SRBC). Results showed that mean values obtained for high and low antibody titres were 7.31 and 2.67 respectively, resulting in the classification of the turkeys into Black high and low, Lavender high and black, and White high and low antibody titres. The genotype’s titre had significant (P <0.05) effect on the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) and basophil (BAS) of the basal haematological parameters. Genotype’s titre had no significant (P >0.05) effect on the primary response haematological parameters. Meanwhile, the primary response haematological parameters to SRBC antigen varied along the genotypes with the WBC increasing drastically in all the genotypes, signifying the presence of an antigen. The study concluded that the F1 turkey poult population studied diverged along the high and low antibody titre in response to SRBC. Thus, the F1 generation of the high antibody titre genotypes (Black high, White high and Lavender high) can be used as foundation stock for selection of local turkeys for high antibody titre.
{"title":"Antibody Response and Haematological Changes of Nigerian Locally Adapted Turkeys to Sheep Red Blood Cells (SRBC)","authors":"A. Ogundero, M. O. Sanda, A. Adenaike, M. Takeet, C. Ikeobi","doi":"10.15835/buasvmcn-asb:2020.0005","DOIUrl":"https://doi.org/10.15835/buasvmcn-asb:2020.0005","url":null,"abstract":"Haemagglutination assay and haematological analysis of 143 poults generated as F1 individuals by artificial insemination from randomly selected turkeys of White, Black and Lavender genotypes which are classified by antibody titre was carried out so as to confirm their antibody titre levels in response to sheep red blood cells (SRBC). Results showed that mean values obtained for high and low antibody titres were 7.31 and 2.67 respectively, resulting in the classification of the turkeys into Black high and low, Lavender high and black, and White high and low antibody titres. The genotype’s titre had significant (P <0.05) effect on the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) and basophil (BAS) of the basal haematological parameters. Genotype’s titre had no significant (P >0.05) effect on the primary response haematological parameters. Meanwhile, the primary response haematological parameters to SRBC antigen varied along the genotypes with the WBC increasing drastically in all the genotypes, signifying the presence of an antigen. The study concluded that the F1 turkey poult population studied diverged along the high and low antibody titre in response to SRBC. Thus, the F1 generation of the high antibody titre genotypes (Black high, White high and Lavender high) can be used as foundation stock for selection of local turkeys for high antibody titre.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42815109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}