首页 > 最新文献

Caryologia最新文献

英文 中文
Effect of Ag Nanoparticles on Morphological and Physio-biochemical Traits of the Medicinal Plant Stevia Rebaudiana 银纳米颗粒对药用植物甜菊菊形态及生理生化特性的影响
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1447
Sherzad R. Abdull, S. Rashid, B. S. Ghafoor, Barzan S. Khdhir
Nowadays, overproduction of secondary metabolites in remedial herbs through giving biotic/abiotic stresses is an interesting area of research. In the current study, the influences of various concentrations of silver nanoparticles (Ag NPs) were evaluated on several morphological and physio-biochemical traits, such as the steviol glycosides level in Stevia. The findings showed that the herbs incubated with 400-ppm Ag NPs own the highest dry and fresh weight of shoot, while those incubated with 80- up to 200- ppm Ag NPs own the highest steviol glycosides content. As a result, we successfully improve the content of stevioside glycoside up to 1.75-fold by applying the 80- up to 200-ppm Ag NPs in Stevia medicinal plant. Moreover, our findings revealed that low concentrations the Ag NPs lead to an increase of glutathione content and total antioxidant capacity, and a decrease of MDA, whereas treatments at higher concentrations induced adverse effects for the plant. As a result, the treatment with Ag NPs low concentrations had a favorable efficacy on physio-biochemical and morphological characteristics of Stevia. These achievements are very promising, because they revealed a considerable capability for the Ag NPs application in enhancing the secondary metabolites in Stevia remedial herb. The present study is the first case assessing the desirable influences of Ag NPs on the Stevia, in regard with shifting of biosynthetic pathway of steviol glycosides in a concentration-dependent manner.
目前,通过给予生物/非生物胁迫治疗草药次生代谢物的过量生产是一个有趣的研究领域。在本研究中,研究了不同浓度的银纳米颗粒(Ag NPs)对甜菊糖中甜菊醇苷含量等形态学和生理生化特性的影响。结果表明,在400-ppm Ag NPs处理下,茎干重和鲜重最高,而在80- 200 ppm Ag NPs处理下,甜菊醇苷含量最高。结果表明,在甜叶菊药用植物中施用80 ~ 200 ppm的Ag NPs,甜叶菊糖苷的含量提高了1.75倍。此外,我们的研究结果表明,低浓度的Ag NPs导致植物谷胱甘肽含量和总抗氧化能力增加,MDA降低,而高浓度处理则对植物产生不利影响。由此可见,低浓度Ag NPs处理对甜菊糖的生理生化和形态特征均有较好的影响。这些研究结果显示了Ag NPs在甜叶菊治疗草药中提高次生代谢产物的应用潜力。本研究首次评估了Ag NPs对甜菊糖的理想影响,即以浓度依赖的方式改变甜菊糖苷的生物合成途径。
{"title":"Effect of Ag Nanoparticles on Morphological and Physio-biochemical Traits of the Medicinal Plant Stevia Rebaudiana","authors":"Sherzad R. Abdull, S. Rashid, B. S. Ghafoor, Barzan S. Khdhir","doi":"10.36253/caryologia-1447","DOIUrl":"https://doi.org/10.36253/caryologia-1447","url":null,"abstract":"Nowadays, overproduction of secondary metabolites in remedial herbs through giving biotic/abiotic stresses is an interesting area of research. In the current study, the influences of various concentrations of silver nanoparticles (Ag NPs) were evaluated on several morphological and physio-biochemical traits, such as the steviol glycosides level in Stevia. The findings showed that the herbs incubated with 400-ppm Ag NPs own the highest dry and fresh weight of shoot, while those incubated with 80- up to 200- ppm Ag NPs own the highest steviol glycosides content. As a result, we successfully improve the content of stevioside glycoside up to 1.75-fold by applying the 80- up to 200-ppm Ag NPs in Stevia medicinal plant. Moreover, our findings revealed that low concentrations the Ag NPs lead to an increase of glutathione content and total antioxidant capacity, and a decrease of MDA, whereas treatments at higher concentrations induced adverse effects for the plant. As a result, the treatment with Ag NPs low concentrations had a favorable efficacy on physio-biochemical and morphological characteristics of Stevia. These achievements are very promising, because they revealed a considerable capability for the Ag NPs application in enhancing the secondary metabolites in Stevia remedial herb. The present study is the first case assessing the desirable influences of Ag NPs on the Stevia, in regard with shifting of biosynthetic pathway of steviol glycosides in a concentration-dependent manner.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"9 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75520705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
First record of nucleus migration in premeiotic antherial cells of Saccharum spontaneum L. (Poaceae) 天然糖(Saccharum spontanum L.)减数分裂前蜂房细胞细胞核迁移的首次记录
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1418
Chandra Bhanu Singh, V. Singhal, M. Kapoor
The occurrence of nucleus migration is reported for the first time in a clone (2n = 64) of ‘Thatch’ grass (Saccharum spontaneum L.) of the family Poaceae. Usually, its premeiotic antherial cells are thin walled, uninucleate and without any trace of chromosome individuality. However, the cells of those anthers that had been affected from flood water stress conditions were anucleated to hexanucleated in varying frequencies. Out of 2567 cells analyzed, two and three cells were noticed to be connected to each other through a well-defined cytoplasmic channel. The nuclei were observed at various stages of their migration in interconnected cells. The remaining cells exhibited a mosaic of anucleate to hexanucleate cells in varying frequencies with a dominance of binucleated condition (43.75%). The anucleate ‘ghost’ cells were much smaller in size than the uninucleate, binucleate and multinucleate cells showing insignificant variation among themselves. The anucleate, binucleate and multinucleate cells appeared to be resulted due to nucleus migration through cytoplasmic channels between two cells. The presence of a nucleus in donor cell united with recipient cell having four nuclei of different sizes, diminutive anucleate cell in the neighbourhood of uninucleate/trinucleate cell or connected with cytoplasmic channel/pentanucleate cell, and disorganizing cytoplasmic channel attached with binucleate/ tetranucleate cell witnessed the accomplishment of nucleus migration. This rare phenomenon of nucleus migration seemed to be triggered by flood water induced stress and facilitated by feeble cell wall. The variation in sizes of nuclei in multinucleate cells might be due to the transfer of nucleus/nuclei of different size(s).  The prominent features of nucleus migration distinguishing it from the cytomixis have been discussed in detail. The syncytes resulted due to nucleus migration might have generated the pollen grains with different genetic constitution resulting into the origin of new intraspecific aneuploids/ polyploids for better adaptability.
本文首次报道了禾科‘茅草’(Saccharum spontaneum L.)无性系(2n = 64)的细胞核迁移现象。通常,其减数分裂前的花药细胞是薄壁的,无核的,没有任何染色体个性的痕迹。然而,受洪水胁迫条件影响的花药细胞以不同的频率无核到六核。在分析的2567个细胞中,有两个和三个细胞通过明确的细胞质通道相互连接。细胞核在相互连接的细胞中迁移的不同阶段被观察到。其余细胞呈现不同频率的无核与六核嵌合体,以双核为主(43.75%)。无核“鬼”细胞的大小比单核、双核和多核细胞小得多,它们之间的差异不显著。无核、双核和多核细胞似乎是由于细胞核通过细胞质通道在两个细胞之间迁移而产生的。在供体细胞中存在一个细胞核与具有四个不同大小细胞核的受体细胞结合,在单核/三核细胞附近或与细胞质通道/五核细胞连接的小型无核细胞,以及与双核/四核细胞连接的散乱的细胞质通道见证了细胞核迁移的完成。这种罕见的细胞核迁移现象似乎是由洪水诱导的应激引起的,并由脆弱的细胞壁促进。多核细胞中细胞核大小的变化可能是由于不同大小的细胞核的转移所致。并详细讨论了细胞核迁移与细胞分裂的显著区别。由于核迁移产生的合子可能产生了不同遗传结构的花粉粒,从而产生新的种内非整倍体/多倍体,以获得更好的适应性。
{"title":"First record of nucleus migration in premeiotic antherial cells of Saccharum spontaneum L. (Poaceae)","authors":"Chandra Bhanu Singh, V. Singhal, M. Kapoor","doi":"10.36253/caryologia-1418","DOIUrl":"https://doi.org/10.36253/caryologia-1418","url":null,"abstract":"The occurrence of nucleus migration is reported for the first time in a clone (2n = 64) of ‘Thatch’ grass (Saccharum spontaneum L.) of the family Poaceae. Usually, its premeiotic antherial cells are thin walled, uninucleate and without any trace of chromosome individuality. However, the cells of those anthers that had been affected from flood water stress conditions were anucleated to hexanucleated in varying frequencies. Out of 2567 cells analyzed, two and three cells were noticed to be connected to each other through a well-defined cytoplasmic channel. The nuclei were observed at various stages of their migration in interconnected cells. The remaining cells exhibited a mosaic of anucleate to hexanucleate cells in varying frequencies with a dominance of binucleated condition (43.75%). The anucleate ‘ghost’ cells were much smaller in size than the uninucleate, binucleate and multinucleate cells showing insignificant variation among themselves. The anucleate, binucleate and multinucleate cells appeared to be resulted due to nucleus migration through cytoplasmic channels between two cells. The presence of a nucleus in donor cell united with recipient cell having four nuclei of different sizes, diminutive anucleate cell in the neighbourhood of uninucleate/trinucleate cell or connected with cytoplasmic channel/pentanucleate cell, and disorganizing cytoplasmic channel attached with binucleate/ tetranucleate cell witnessed the accomplishment of nucleus migration. This rare phenomenon of nucleus migration seemed to be triggered by flood water induced stress and facilitated by feeble cell wall. The variation in sizes of nuclei in multinucleate cells might be due to the transfer of nucleus/nuclei of different size(s).  The prominent features of nucleus migration distinguishing it from the cytomixis have been discussed in detail. The syncytes resulted due to nucleus migration might have generated the pollen grains with different genetic constitution resulting into the origin of new intraspecific aneuploids/ polyploids for better adaptability.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"14 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82375698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SCoT molecular markers are efficient in genetic fingerprinting of pomegranate (Punica granatum L.) cultivars SCoT分子标记是一种高效的石榴品种遗传指纹分析方法
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1567
Shiva Shahsavari, Z. Noormohammadi, M. Sheidai, F. Farahani, Mohammad Reza Vazifeshenas
The pomegranate is an economically important fruit plant species which has been utilized since ancient time as a source of food and medicine by mankind. This plant although is cultivated in certain geographical regions, but its fruits are imported and sold throughout the world. Iran is the center of origin for pomegranate and contains huge number of known cultivars. However, genetic studied on these cultivars are very limited and much detailed information has to be produced for better hybridization and breeding tasks in the country. A fingerprinting study was performed on 178 Punica trees in 47 known cultivars by using SCoT molecular markers. We obtained 61 SCoT bands/ loci which were used for genetic diversity analyses and grouping of the cultivars. A low genetic variability was obtained within and among Punica cultivars, but as revealed by AMOVA, this was quiet enough to produce significant genetic difference among them. DAPC analysis revealed a trace of genetic admixture among the cultivars either due to gene flow or as a result of common ancestral shared alleles. Discriminating SCoT loci may be used in germplasm evaluation of Punica. The genetic difference of these cultivars can be utilized for hybridization and breeding programs. 
石榴是一种经济上重要的水果植物,自古以来就被人类用作食物和药物的来源。这种植物虽然只在某些地理区域种植,但其果实却进口并销往世界各地。伊朗是石榴的原产地中心,拥有大量的已知品种。然而,对这些品种的遗传研究非常有限,必须提供更详细的信息,以便在该国更好地进行杂交和育种任务。利用SCoT分子标记对47个已知品种的178棵Punica树进行了指纹图谱分析。我们获得61个SCoT条带/位点,用于品种的遗传多样性分析和分类。品种间和品种内部的遗传变异性较低,但AMOVA分析表明,这一差异足以产生显著的遗传差异。DAPC分析显示,由于基因流动或共同祖先共有等位基因,品种间存在遗传混合的痕迹。鉴别SCoT位点可用于Punica种质资源评价。这些品种的遗传差异可用于杂交和育种计划。
{"title":"SCoT molecular markers are efficient in genetic fingerprinting of pomegranate (Punica granatum L.) cultivars","authors":"Shiva Shahsavari, Z. Noormohammadi, M. Sheidai, F. Farahani, Mohammad Reza Vazifeshenas","doi":"10.36253/caryologia-1567","DOIUrl":"https://doi.org/10.36253/caryologia-1567","url":null,"abstract":"The pomegranate is an economically important fruit plant species which has been utilized since ancient time as a source of food and medicine by mankind. This plant although is cultivated in certain geographical regions, but its fruits are imported and sold throughout the world. Iran is the center of origin for pomegranate and contains huge number of known cultivars. However, genetic studied on these cultivars are very limited and much detailed information has to be produced for better hybridization and breeding tasks in the country. A fingerprinting study was performed on 178 Punica trees in 47 known cultivars by using SCoT molecular markers. We obtained 61 SCoT bands/ loci which were used for genetic diversity analyses and grouping of the cultivars. A low genetic variability was obtained within and among Punica cultivars, but as revealed by AMOVA, this was quiet enough to produce significant genetic difference among them. DAPC analysis revealed a trace of genetic admixture among the cultivars either due to gene flow or as a result of common ancestral shared alleles. Discriminating SCoT loci may be used in germplasm evaluation of Punica. The genetic difference of these cultivars can be utilized for hybridization and breeding programs. ","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"33 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78628215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First Report on Classical and Molecular Cytogenetics of Doi Inthanon Bent-toed Gecko, Cyrtodactylus inthanon Kunya et al., 2015 (Squamata: Gekkonidae) in Thailand Doi Inthanon弯趾壁虎,Cyrtodactylus Inthanon Kunya et al., 2015(鳞目:弯趾壁虎科)在泰国的经典和分子细胞遗传学首次报道
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1514
S. Prasopsin, Nawarat Muanglen, Sukhonthip Ditcharoen, C. Suwannapoom, A. Tanomtong, Weera Thongnetr
This study analyzed the karyotype of Cyrtodactylus inthanon Kunya et al., 2015 from Doi Inthanon, Chiang Mai Province, northern Thailand. The metaphase and meiotic chromosome preparations were obtained by squash technique from bone marrow and testes, respectively. The chromosomes were stained by Giemsa staining, Ag-NOR banding and molecular cytogenetics with ?uorescence in situ hybridization (FISH) using microsatellites d(CA)15, d(GC)15, d(CAG)10 and d(GAA)10 as probes. The results showed the diploid chromosome number (2n) of 40. The chromosome types of metacentric, submetacentric, acrocentric and telocentric chromosomes were 12-4-2-22, respectively. The Ag-NORs banding technique provides the pair of nucleolar organizer regions (NORs) on the telomeric region of the long arm of acrocentric pair 12. There are no sex differences in karyotypes between males and females. We also found that during metaphase I on meiosis of C. inthanon, the homologous chromosomes appeared synapsis of 20 bivalents. The microsatellite d(CA)15 signals were located on the sub-centromeric region of the metacentric pair 10, whereas the d(GC)15, d(CAG)10 and d(GAA)10 repeats are highly accumulated throughout almost all entire chromosomes. The karyotype formula is as follows: C. inthanon (2n = 40), Lm2 + Lsm4 + Lt6 + Mm2 + Mt8 + Sm8 + Sa2 + St8.
本研究分析了来自泰国北部清迈省Doi inthanon的Cyrtodactylus inthanon Kunya et al., 2015的核型。用挤压法分别从骨髓和睾丸中获得中期染色体和减数分裂染色体。以微卫星d(CA)15、d(GC)15、d(CAG)10和d(GAA)10为探针,采用Giemsa染色、Ag-NOR显带和荧光原位杂交(FISH)进行分子细胞遗传学染色。结果显示,二倍体染色体数(2n)为40。常着丝、亚常着丝、近着丝和远着丝染色体类型分别为12-4-22。Ag-NORs带带技术提供了一对核仁组织区(NORs)在端粒区域的长臂对12。男性和女性的核型没有性别差异。我们还发现,在茜草减数分裂中期,同源染色体出现了20个二价的突触。微卫星d(CA)15信号位于定中心对10的亚着丝粒区,而d(GC)15、d(CAG)10和d(GAA)10重复序列则高度积累在几乎所有的整条染色体上。核型公式为:C. inthanon (2n = 40), Lm2 + Lsm4 + Lt6 + Mm2 + Mt8 + Sm8 + Sa2 + St8。
{"title":"First Report on Classical and Molecular Cytogenetics of Doi Inthanon Bent-toed Gecko, Cyrtodactylus inthanon Kunya et al., 2015 (Squamata: Gekkonidae) in Thailand","authors":"S. Prasopsin, Nawarat Muanglen, Sukhonthip Ditcharoen, C. Suwannapoom, A. Tanomtong, Weera Thongnetr","doi":"10.36253/caryologia-1514","DOIUrl":"https://doi.org/10.36253/caryologia-1514","url":null,"abstract":"This study analyzed the karyotype of Cyrtodactylus inthanon Kunya et al., 2015 from Doi Inthanon, Chiang Mai Province, northern Thailand. The metaphase and meiotic chromosome preparations were obtained by squash technique from bone marrow and testes, respectively. The chromosomes were stained by Giemsa staining, Ag-NOR banding and molecular cytogenetics with ?uorescence in situ hybridization (FISH) using microsatellites d(CA)15, d(GC)15, d(CAG)10 and d(GAA)10 as probes. The results showed the diploid chromosome number (2n) of 40. The chromosome types of metacentric, submetacentric, acrocentric and telocentric chromosomes were 12-4-2-22, respectively. The Ag-NORs banding technique provides the pair of nucleolar organizer regions (NORs) on the telomeric region of the long arm of acrocentric pair 12. There are no sex differences in karyotypes between males and females. We also found that during metaphase I on meiosis of C. inthanon, the homologous chromosomes appeared synapsis of 20 bivalents. The microsatellite d(CA)15 signals were located on the sub-centromeric region of the metacentric pair 10, whereas the d(GC)15, d(CAG)10 and d(GAA)10 repeats are highly accumulated throughout almost all entire chromosomes. The karyotype formula is as follows: C. inthanon (2n = 40), Lm2 + Lsm4 + Lt6 + Mm2 + Mt8 + Sm8 + Sa2 + St8.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"43 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84921970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chromosomal description of three Dixonius (Squamata, Gekkonidae) from Thailand 文章标题泰国三种刺蝇(鳞目,刺蝇科)染色体特征
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1432
I. Patawang, S. Prasopsin, C. Suwannapoom, A. Tanomtong, Puntivar Keawmad, Weera Thongnetr
Chromosomal characteristics and karyological analysis of three Dixonius, including D. hangseesom, D. siamensis and D. melanostictus, from Thailand were studied. Chromosome preparations were conducted by squash technique from bone marrow and testis. Conventional Giemsa’s staining and Ag-NOR banding techniques were applied to stain the chromosome. The results showed that the diploid chromosomes are 2n=40, for D. hangseesom and D. siamensis; and 2n=42, for D. melanostictus. The fundamental number (NF) is 42 in D. hangseesom and D. siamensis and 44 in D. melanostictus. The types of chromosomes were 2 metacentrics and 38 telocentrics for D. hangseesom and D. siamensis, while the karyotype of D. melanostictus comprised 2 acrocentrics and 40 telocentrics. In the D. hangseesom and D. siamensis, NORs are located to the near centromere on long arm of the telocentric chromosome pair 13. Although, the NORs of D. melanostictus are situated on the subtelomeric region of telocentric chromosome pair 8. There are no sex differences in karyotypes between males and females of these three geckos species. We found that during metaphase I and metaphase II on male meiosis of the D. hangseesom and D. siamensis, the homologous chromosomes showed synapsis of 20 bivalents and 20 haploid chromosomes (n=20). Moreover, metaphase I and metaphase II of the male D. melanostictus showed synapsis of 21 bivalents and 21 haploid chromosomes (n=21). Their karyotype formulas is as follows: D. hangseesom (2n=40): Lm2 + Lt2 + Mt4 + St32, D. siamensis (2n=40): Lm2 + Lt6 + Mt4 + St28, and D. melanostictus (2n=42): La2 + Lt12 + Mt4 + St24.
研究了来自泰国的3种Dixonius (D. hangseesom, D. siamensis和D. melanotictus)的染色体特征和核学分析。用挤压法制备骨髓和睾丸染色体。采用常规吉姆萨染色和Ag-NOR显带技术对染色体进行染色。结果表明:海南稻和暹罗稻的二倍体染色体数为2n=40;和2n=42,对于D. melanotictus。D. hangseesom和D. siamensis的基数为42,D. melanotictus的基数为44。hangseesom和siamensis的染色体类型为2个正中心和38个远中心,而melanotictus的染色体类型为2个正中心和40个远中心。在D. hangseesom和D. siamensis中,NORs位于远心染色体13对长臂上的着丝粒附近。然而,黑纹龙虎的NORs位于远心染色体8对的亚端粒区。这三种壁虎的雌雄核型没有性别差异。结果表明,在雄性减数分裂的中期I和中期II,同源染色体出现了20条二价染色体和20条单倍体染色体的突触(n=20)。此外,在雄性黑纹金鼠的中期I和中期II,有21条二价染色体和21条单倍体染色体(n=21)发生突触。其核型公式为:D. hangseesom (2n=40): Lm2 + Lt2 + Mt4 + St32, D. siamensis (2n=40): Lm2 + Lt6 + Mt4 + St28, D. melanotictus (2n=42): La2 + Lt12 + Mt4 + St24。
{"title":"Chromosomal description of three Dixonius (Squamata, Gekkonidae) from Thailand","authors":"I. Patawang, S. Prasopsin, C. Suwannapoom, A. Tanomtong, Puntivar Keawmad, Weera Thongnetr","doi":"10.36253/caryologia-1432","DOIUrl":"https://doi.org/10.36253/caryologia-1432","url":null,"abstract":"Chromosomal characteristics and karyological analysis of three Dixonius, including D. hangseesom, D. siamensis and D. melanostictus, from Thailand were studied. Chromosome preparations were conducted by squash technique from bone marrow and testis. Conventional Giemsa’s staining and Ag-NOR banding techniques were applied to stain the chromosome. The results showed that the diploid chromosomes are 2n=40, for D. hangseesom and D. siamensis; and 2n=42, for D. melanostictus. The fundamental number (NF) is 42 in D. hangseesom and D. siamensis and 44 in D. melanostictus. The types of chromosomes were 2 metacentrics and 38 telocentrics for D. hangseesom and D. siamensis, while the karyotype of D. melanostictus comprised 2 acrocentrics and 40 telocentrics. In the D. hangseesom and D. siamensis, NORs are located to the near centromere on long arm of the telocentric chromosome pair 13. Although, the NORs of D. melanostictus are situated on the subtelomeric region of telocentric chromosome pair 8. There are no sex differences in karyotypes between males and females of these three geckos species. We found that during metaphase I and metaphase II on male meiosis of the D. hangseesom and D. siamensis, the homologous chromosomes showed synapsis of 20 bivalents and 20 haploid chromosomes (n=20). Moreover, metaphase I and metaphase II of the male D. melanostictus showed synapsis of 21 bivalents and 21 haploid chromosomes (n=21). Their karyotype formulas is as follows: D. hangseesom (2n=40): Lm2 + Lt2 + Mt4 + St32, D. siamensis (2n=40): Lm2 + Lt6 + Mt4 + St28, and D. melanostictus (2n=42): La2 + Lt12 + Mt4 + St24.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"184 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88096314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative chromosome mapping of repetitive DNA in four minnow fishes (Cyprinidae, Cypriniformes) 四种鲦鱼(鲤科,鲤形目)重复DNA的比较染色体定位
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1523
S. Aiumsumang, P. Chaiyasan, K. Khoomsab, W. Supiwong, A. Tanomtong, S. Phimphan
The present study focused on the repetitive DNA of the chromosome in four minnow fishes from the genera Danio Hamilton, 1822, Devario Heckel, 1843 and Rasbora Bleeker, 1859. Chromosomes were analysed using fluorescence in situ hybridization (FISH) with microsatellite probes including (CA)15, (CAC)10, (CGG)10, (GC)15 and (TA)15 staining. All species retained the diploid chromosome number 2n = 50 in male and female. The microsatellite sequences were mapped in the chromosomes of Danio albolineatus (Blyth, 1860), Devario regina (Fowler, 1934), Rasbora aurotaenia Tirant, 1885 and R. paviana Tirant, 1885. In most cases, the microsatellite was dispersed in the chromosome with conspicuous markings in the telomeric region and the whole genome, which suggests that sequences contribute to chromosome structure and may have played a role in the relationship of this fish group. The comparative genome mapping data presented here provide novel information on the structure and organisation of the repetitive DNA region of the minnow’s genome and contribute to a better understanding of the genomes of these minnows.
本研究的重点是4种小鱼的染色体重复DNA,分别来自Danio Hamilton(1822)、Devario Heckel(1843)和Rasbora Bleeker(1859)。采用荧光原位杂交(FISH)技术对染色体进行分析,微卫星探针包括(CA)15、(CAC)10、(CGG)10、(GC)15和(TA)15染色。雌雄均保留二倍体染色体数目2n = 50。微卫星序列分别定位于Danio albolineatus (Blyth, 1860)、Devario regina (Fowler, 1934)、Rasbora aurotaenia Tirant(1885)和R. paviana Tirant(1885)的染色体上。在大多数情况下,微卫星分散在染色体上,在端粒区和整个基因组中有明显的标记,这表明序列有助于染色体结构,并可能在该鱼类群体的关系中发挥作用。本文提出的比较基因组图谱数据提供了关于鲦鱼基因组重复DNA区域的结构和组织的新信息,有助于更好地了解这些鲦鱼的基因组。
{"title":"Comparative chromosome mapping of repetitive DNA in four minnow fishes (Cyprinidae, Cypriniformes)","authors":"S. Aiumsumang, P. Chaiyasan, K. Khoomsab, W. Supiwong, A. Tanomtong, S. Phimphan","doi":"10.36253/caryologia-1523","DOIUrl":"https://doi.org/10.36253/caryologia-1523","url":null,"abstract":"The present study focused on the repetitive DNA of the chromosome in four minnow fishes from the genera Danio Hamilton, 1822, Devario Heckel, 1843 and Rasbora Bleeker, 1859. Chromosomes were analysed using fluorescence in situ hybridization (FISH) with microsatellite probes including (CA)15, (CAC)10, (CGG)10, (GC)15 and (TA)15 staining. All species retained the diploid chromosome number 2n = 50 in male and female. The microsatellite sequences were mapped in the chromosomes of Danio albolineatus (Blyth, 1860), Devario regina (Fowler, 1934), Rasbora aurotaenia Tirant, 1885 and R. paviana Tirant, 1885. In most cases, the microsatellite was dispersed in the chromosome with conspicuous markings in the telomeric region and the whole genome, which suggests that sequences contribute to chromosome structure and may have played a role in the relationship of this fish group. The comparative genome mapping data presented here provide novel information on the structure and organisation of the repetitive DNA region of the minnow’s genome and contribute to a better understanding of the genomes of these minnows.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"12 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80730649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphometric analysis and genetic diversity in Hypericum L. using sequence related amplified polymorphism 利用序列相关扩增多态性分析金丝桃的形态计量学及遗传多样性
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1515
W. Cao, X. Chen, Z. Cao
There are about 484 species of Hypericum in the Guttiferae family, which includes Hypericoideae. In Iran, species of this genus are mainly found in the north, northwest, and center of the country, and they are key contributors to the floral elements of the Hyrcanian mountains, Irano-Turanian, and Mediterranean regions (such as the Zagros). Medicinal, commercial, and horticultural values are associated with these plants.The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Hypericum species, were studied through a combination of morphological and molecular data. Eighty-five individuals related to 7 Hypericum were collected in 6 provinces. A total of 76 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Hypericum species. These bands were produced with the combinations of 5 selective primers. The total number of amplified fragments ranged from 10 to 20. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, H. perforatum and H. asperulum had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Hypericum species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran.Genus Hypericum (Guttiferae, Hypericoideae) is perennial, belonging to the Hypericaceae family, having 484 species in forms of trees, shrubs, and herbs, distributed in 36 taxonomic sections. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Hypericum species, were studied through  a combination of morphological and molecular data. Eighty-five individuals related to 7 Hypericum were collected in 6 provinces. A total of 76 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Hypericum species. These bands were produced with the combinations of 5 selective primers. The total number of amplified fragments ranged from 10 to 20. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, H. perforatum and H. asperulum had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in  Hypericum species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purpos
木耳科金丝桃属植物约有484种,其中包括金丝桃科。在伊朗,该属的物种主要分布在该国的北部,西北部和中部,它们是赫卡尼亚山脉,伊朗-图拉尼亚和地中海地区(如扎格罗斯)的花卉元素的关键贡献者。药用、商业和园艺价值与这些植物有关。通过序列相关扩增多态性评估遗传多样性。为了揭示金丝桃属植物的遗传多样性和物种特征,采用形态和分子相结合的方法对其进行了研究。在6个省共收集到7种金丝桃属植物85株。对7种金丝桃进行聚合酶链反应扩增(PCR),共扩增出76条(NTL) DNA条带。这些条带由5条选择性引物组合而成。扩增片段总数在10 ~ 20个之间。SRAP (Sequence-related amplified polymorphism,序列相关扩增多态性)标记分析结果显示,贯叶莲与asperulum的相似性最低。本研究还发现了距离隔离的显著特征(Mantel测试结果)。目前的研究结果表明,序列相关的扩增多态性具有鉴定和破译金丝桃属植物遗传亲和力的潜力。目前的结果对生物多样性和保护计划具有启示意义。此外,目前的研究结果可以为选择适合伊朗饲草和牧场用途的生态型铺平道路。金丝桃属(gutiferae,金丝桃科)是一种多年生植物,属于金丝桃科,有乔木、灌木和草本植物484种,分布在36个分类区。通过序列相关扩增多态性评估遗传多样性。为了揭示金丝桃属植物的遗传多样性和物种特征,采用形态和分子相结合的方法对其进行了研究。在6个省共收集到7种金丝桃属植物85株。对7种金丝桃进行聚合酶链反应扩增(PCR),共扩增出76条(NTL) DNA条带。这些条带由5条选择性引物组合而成。扩增片段总数在10 ~ 20个之间。SRAP (Sequence-related amplified polymorphism,序列相关扩增多态性)标记分析结果显示,贯叶莲与asperulum的相似性最低。本研究还发现了距离隔离的显著特征(Mantel测试结果)。目前的研究结果表明,序列相关的扩增多态性具有鉴定和破译金丝桃属植物遗传亲和力的潜力。目前的结果对生物多样性和保护计划具有启示意义。此外,目前的研究结果可以为选择适合伊朗饲草和牧场用途的生态型铺平道路。
{"title":"Morphometric analysis and genetic diversity in Hypericum L. using sequence related amplified polymorphism","authors":"W. Cao, X. Chen, Z. Cao","doi":"10.36253/caryologia-1515","DOIUrl":"https://doi.org/10.36253/caryologia-1515","url":null,"abstract":"There are about 484 species of Hypericum in the Guttiferae family, which includes Hypericoideae. In Iran, species of this genus are mainly found in the north, northwest, and center of the country, and they are key contributors to the floral elements of the Hyrcanian mountains, Irano-Turanian, and Mediterranean regions (such as the Zagros). Medicinal, commercial, and horticultural values are associated with these plants.The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Hypericum species, were studied through a combination of morphological and molecular data. Eighty-five individuals related to 7 Hypericum were collected in 6 provinces. A total of 76 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Hypericum species. These bands were produced with the combinations of 5 selective primers. The total number of amplified fragments ranged from 10 to 20. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, H. perforatum and H. asperulum had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Hypericum species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran.\u0000Genus Hypericum (Guttiferae, Hypericoideae) is perennial, belonging to the Hypericaceae family, having 484 species in forms of trees, shrubs, and herbs, distributed in 36 taxonomic sections. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Hypericum species, were studied through  a combination of morphological and molecular data. Eighty-five individuals related to 7 Hypericum were collected in 6 provinces. A total of 76 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Hypericum species. These bands were produced with the combinations of 5 selective primers. The total number of amplified fragments ranged from 10 to 20. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, H. perforatum and H. asperulum had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in  Hypericum species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purpos","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"41 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75646313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic Characterization of Salicornia persica Akhani (Chenopodiaceae) Assessed Using Random Amplified Polymorphic DNA 利用随机扩增多态DNA评估阿卡尼水杨的遗传特征
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1493
Zhu Lin, H. Khodayari
Salicornia is a wild and annual (biennial, in some species) halophytic plant from Chenopodiaceae family that grows near salt marshes and salted wetlands or the vicinity of coastal areas in Asia, North America, and the Middle East. It is also grown in semi-arid areas of Iran such as Isfahan, Fars, and Yazd provinces. Genetic variability and populations, structure were studied in 10 geographical populations of Salicornia persica. Genetic diversity parameters were determined in these populations. 10 of 20 random amplified polymorphic DNA (RAPD) primers produced 146 reproducible bands with average of 14.6 bands per primer and 88.67% of polymorphism. OPA10 primer showed the highest number of effective allele (Ne), Shannon index (I) and genetic diversity (H). The highest values of genetic diversity in RAPD markers were obtained in Esfahan, Nain, it is 70km to Varzaneh populations. WARD trees of RAPD data grouped the populations in two different clusters/groups, indicating their genetic difference which is discussed in details. The results of this study showed that the level of genetic variation in Salicornia persica is relatively low. WARD-based dendrogram showed a close relationship between members of Esfahan, Varzaneh and Esfahan, the river of Zayanderud at Varzaneh while the Yazd, Meybud and Yazd, Nádüshan protected population differ the most from the other populations. Principal component analysis, however, showed some minor differences with WARD-based dendrograms.
盐角草是一种野生的一年生盐生植物(某些种类为两年生),来自藜科,生长在亚洲、北美和中东的盐沼和盐渍湿地附近或沿海地区。它也生长在伊朗的半干旱地区,如伊斯法罕、法尔斯和亚兹德省。对10个地理居群的遗传变异和居群结构进行了研究。测定了这些群体的遗传多样性参数。20条随机扩增多态性DNA (RAPD)引物中,10条引物产生146条可重复条带,平均14.6条,多态性率为88.67%。OPA10引物有效等位基因数(Ne)、Shannon指数(I)和遗传多样性(H)最高,RAPD标记遗传多样性最高的是Esfahan和Nain,距离Varzaneh群体70km。RAPD数据的WARD树将群体分为两个不同的聚类/组,表明了它们的遗传差异,并对此进行了详细的讨论。本研究结果表明,海角的遗传变异水平相对较低。基于ward的树形图显示Esfahan, Varzaneh和Esfahan (Varzaneh的Zayanderud河)成员之间的关系密切,而Yazd, Meybud和Yazd, Nádüshan受保护种群与其他种群差异最大。然而,主成分分析显示了与基于ward的树突图的一些细微差异。
{"title":"Genetic Characterization of Salicornia persica Akhani (Chenopodiaceae) Assessed Using Random Amplified Polymorphic DNA","authors":"Zhu Lin, H. Khodayari","doi":"10.36253/caryologia-1493","DOIUrl":"https://doi.org/10.36253/caryologia-1493","url":null,"abstract":"Salicornia is a wild and annual (biennial, in some species) halophytic plant from Chenopodiaceae family that grows near salt marshes and salted wetlands or the vicinity of coastal areas in Asia, North America, and the Middle East. It is also grown in semi-arid areas of Iran such as Isfahan, Fars, and Yazd provinces. Genetic variability and populations, structure were studied in 10 geographical populations of Salicornia persica. Genetic diversity parameters were determined in these populations. 10 of 20 random amplified polymorphic DNA (RAPD) primers produced 146 reproducible bands with average of 14.6 bands per primer and 88.67% of polymorphism. OPA10 primer showed the highest number of effective allele (Ne), Shannon index (I) and genetic diversity (H). The highest values of genetic diversity in RAPD markers were obtained in Esfahan, Nain, it is 70km to Varzaneh populations. WARD trees of RAPD data grouped the populations in two different clusters/groups, indicating their genetic difference which is discussed in details. The results of this study showed that the level of genetic variation in Salicornia persica is relatively low. WARD-based dendrogram showed a close relationship between members of Esfahan, Varzaneh and Esfahan, the river of Zayanderud at Varzaneh while the Yazd, Meybud and Yazd, Nádüshan protected population differ the most from the other populations. Principal component analysis, however, showed some minor differences with WARD-based dendrograms.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"59 8","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72375431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Classical chromosome features and microsatellites repeat in Gekko petricolus (Reptilia, Gekkonidae) from Thailand 泰国壁虎的经典染色体特征和微卫星重复
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1544
Weera Thongnetr, S. Aiumsumang, A. Tanomtong, S. Phimphan
The objectives of this study were to examine size, shape, diploid number (2n), fundamental number (NF), NORs region, and distribution of microsatellite by using Fluorescence in situ hybridization technique (FISH) and to establish the karyotype and standard idiogram of sandstone geckos (Gekko petricolus Taylor, 1962). Sandstone gecko distributed in the sandstone mountains in Laos, Cambodia, and Thailand. Five male and five female specimens were collected  from Ubon Ratchathani and Mukdahan provinces, Thailand. The metaphase cells were directly prepared from the bone marrow cells. Chromosomes were stained by conventional staining, NORs-banding and FISH techniques. The results found that the diploid number was 38 chromosomes. The fundamental number was 54. The karyotype composed of 4 large metacentric, 4 large acrocentric, 2 large telocentric, 4 medium acrocentric, 2 medium telocentric, 2 small submetacentric, 2 small acrocentric and 18 small telocentric chromosomes. No morphological difference was identified between sex chromosomes of male and female specimens. The NORs appeared to telomere of the long arm of chromosome pair 17. The study displayed that the distribution of microsatellite using (CA)15 and (GAA)10 probes distributed throughout the genome. However, (CA)15 sequences concentrated in the telomere. The karyotype formula G. petricolus is as follow: 2n (38) = Lm4+La4+Lt2+Ma4+Mt2+Ssm2+Sa2+St18.
本研究利用荧光原位杂交技术(FISH)对砂岩壁虎的大小、形状、二倍体数(2n)、基本数(NF)、NORs区和微卫星分布进行了研究,建立了砂岩壁虎的核型和标准形态图(Gekko petricolus Taylor, 1962)。砂岩壁虎分布在老挝、柬埔寨和泰国的砂岩山区。在泰国乌汶拉差他尼省和Mukdahan省采集了5只雄性和5只雌性标本。中期细胞直接由骨髓细胞制备。染色体染色采用常规染色、nors显带和FISH技术。结果发现二倍体数目为38条染色体。基本数字是54。核型由4条大正中心、4条大正中心、2条大远中心、4条中正中心、2条中远中心、2条小亚正中心、2条小正中心和18条小远中心染色体组成。雌雄标本的性染色体形态无差异。NORs出现在17号染色体长臂端粒上。研究表明,利用(CA)15和(GAA)10探针,微卫星分布在整个基因组中。然而,(CA)15序列集中在端粒。核型公式为:2n (38) = Lm4+La4+Lt2+Ma4+Mt2+Ssm2+Sa2+St18。
{"title":"Classical chromosome features and microsatellites repeat in Gekko petricolus (Reptilia, Gekkonidae) from Thailand","authors":"Weera Thongnetr, S. Aiumsumang, A. Tanomtong, S. Phimphan","doi":"10.36253/caryologia-1544","DOIUrl":"https://doi.org/10.36253/caryologia-1544","url":null,"abstract":"The objectives of this study were to examine size, shape, diploid number (2n), fundamental number (NF), NORs region, and distribution of microsatellite by using Fluorescence in situ hybridization technique (FISH) and to establish the karyotype and standard idiogram of sandstone geckos (Gekko petricolus Taylor, 1962). Sandstone gecko distributed in the sandstone mountains in Laos, Cambodia, and Thailand. Five male and five female specimens were collected  from Ubon Ratchathani and Mukdahan provinces, Thailand. The metaphase cells were directly prepared from the bone marrow cells. Chromosomes were stained by conventional staining, NORs-banding and FISH techniques. The results found that the diploid number was 38 chromosomes. The fundamental number was 54. The karyotype composed of 4 large metacentric, 4 large acrocentric, 2 large telocentric, 4 medium acrocentric, 2 medium telocentric, 2 small submetacentric, 2 small acrocentric and 18 small telocentric chromosomes. No morphological difference was identified between sex chromosomes of male and female specimens. The NORs appeared to telomere of the long arm of chromosome pair 17. The study displayed that the distribution of microsatellite using (CA)15 and (GAA)10 probes distributed throughout the genome. However, (CA)15 sequences concentrated in the telomere. The karyotype formula G. petricolus is as follow: 2n (38) = Lm4+La4+Lt2+Ma4+Mt2+Ssm2+Sa2+St18.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"24 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75523276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Population Differentiation and Gene Flow of Salicornia persica Akhani (Chenopodiaceae) 藜科水杨的种群分化与基因流动
IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2022-09-21 DOI: 10.36253/caryologia-1541
Xiaojun Zhang, Lingyan Bai, S. Esfandani-Bozchaloyi
The genus Salicornia (Amaranthaceae) was established by Linnaeus. Commonly known as ‘glassworts’, the species of the genus are articulated succulent herbs with cortical palisade, opposite decussate scale-leaves, thyrsoid cymes, flowers packed in cauline depressions and the diaspore composed of l-seeded utricle. Therefore, due to the importance of the plant species, we performed a combination of morphological and molecular data analyses on this species. A total of 72 randomly collected plants from 8 natural populations in 2 provinces were evaluated using ISSR markers and morphological traits. Analysis of molecular variance (AMOVA) test revealed significant genetic difference among the studied populations, and also showed that 45% of total genetic variability was due to the diversity within the population, while 55% was due to the genetic differentiation among populations. A total number of 158 bands were detected by ISSR primers, of which 144 (89%) bands with an average of 14.4 bands per primer were polymorphic. The Percentage of Polymorphic Bands (PPB) ranged from 70% (ISSR-7) to 100% (ISSR-1, ISSR-4 and ISSR-5). The average Polymorphic Information Content (PIC), Shannon’s Information index (I), and Number of effective alleles (Ne) were 0.49, 0.28, and 1.09, respectively.
林奈建立了苋属(苋科)。通常被称为“玻璃草”,属的物种是关节多肉草本植物,具有皮质栅栏,对生的相互对生的鳞状叶,伞形聚伞花序,花排列在茎的凹陷处,一水硬体由l-种子胞室组成。因此,由于该植物物种的重要性,我们对该物种进行了形态学和分子数据的结合分析。利用ISSR标记和形态性状对2省8个自然居群中72株植物进行了评价。分子方差分析(AMOVA)表明,群体间遗传差异显著,群体内多样性占总遗传变异的45%,群体间遗传分化占55%。ISSR引物共检测到158条条带,其中多态性条带144条(89%),平均每个引物14.4条。多态性带百分比(PPB)从70% (ISSR-7)到100% (ISSR-1、ISSR-4和ISSR-5)不等。平均多态性信息含量(PIC)、香农信息指数(I)和有效等位基因数(Ne)分别为0.49、0.28和1.09。
{"title":"Population Differentiation and Gene Flow of Salicornia persica Akhani (Chenopodiaceae)","authors":"Xiaojun Zhang, Lingyan Bai, S. Esfandani-Bozchaloyi","doi":"10.36253/caryologia-1541","DOIUrl":"https://doi.org/10.36253/caryologia-1541","url":null,"abstract":"The genus Salicornia (Amaranthaceae) was established by Linnaeus. Commonly known as ‘glassworts’, the species of the genus are articulated succulent herbs with cortical palisade, opposite decussate scale-leaves, thyrsoid cymes, flowers packed in cauline depressions and the diaspore composed of l-seeded utricle. Therefore, due to the importance of the plant species, we performed a combination of morphological and molecular data analyses on this species. A total of 72 randomly collected plants from 8 natural populations in 2 provinces were evaluated using ISSR markers and morphological traits. Analysis of molecular variance (AMOVA) test revealed significant genetic difference among the studied populations, and also showed that 45% of total genetic variability was due to the diversity within the population, while 55% was due to the genetic differentiation among populations. A total number of 158 bands were detected by ISSR primers, of which 144 (89%) bands with an average of 14.4 bands per primer were polymorphic. The Percentage of Polymorphic Bands (PPB) ranged from 70% (ISSR-7) to 100% (ISSR-1, ISSR-4 and ISSR-5). The average Polymorphic Information Content (PIC), Shannon’s Information index (I), and Number of effective alleles (Ne) were 0.49, 0.28, and 1.09, respectively.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"91 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86970614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Caryologia
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1