Pub Date : 2022-11-25DOI: 10.22627/2072-8107-2022-21-4-62-66
V. Aksenova, N. Klevno, A. Kazakov, A. Pakhlavonova, E. A. Sokolskaya, S. M. Kavtarashvili, V. Romanenko, N. Stepanenko
Parasitic infections are widespread throughout the world and are especially common in childhood. The absence of pathognomonic symptoms in liver diseases is characteristic. We presented clinical observation with asymptomatic echinococcosis with damage to the respiratory organs and liver. During the fluorographic examination, changes in the lungs were revealed, in connection with which she was sent for a consultation with a phthisiatrician. In the TB hospital, with the help of a comprehensive examination and modern telemedicine technologies, changes were identified in lungs and in the liver. This clinical observation showed that it is necessary to conduct a comprehensive examination before prescribing specific anti-tuberculosis treatment. Increasing physicians' knowledge of parasitic diseases could increase the early detection.
{"title":"Parasitic infections in the practice of a phthisiologist (clinical case)","authors":"V. Aksenova, N. Klevno, A. Kazakov, A. Pakhlavonova, E. A. Sokolskaya, S. M. Kavtarashvili, V. Romanenko, N. Stepanenko","doi":"10.22627/2072-8107-2022-21-4-62-66","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-62-66","url":null,"abstract":"Parasitic infections are widespread throughout the world and are especially common in childhood. The absence of pathognomonic symptoms in liver diseases is characteristic. We presented clinical observation with asymptomatic echinococcosis with damage to the respiratory organs and liver. During the fluorographic examination, changes in the lungs were revealed, in connection with which she was sent for a consultation with a phthisiatrician. In the TB hospital, with the help of a comprehensive examination and modern telemedicine technologies, changes were identified in lungs and in the liver. This clinical observation showed that it is necessary to conduct a comprehensive examination before prescribing specific anti-tuberculosis treatment. Increasing physicians' knowledge of parasitic diseases could increase the early detection.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77840876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.22627/2072-8107-2022-21-4-53-56
T. A. Britkova, K. E. Panteleev, O. A. Pazinenko
Aim: to study the structure, features of the findings and treatment of mixed infections in children at the present stage.Materials and methods. 85 case histories of children aged 5 months to 17 years and 10 months were examined, the final diagnosis of which included 2 or more infections on the basis of the children's infectious diseases department City Clinical Hospital № 7 of Izhevsk. Children with acute infectious pathology (acute intestinal infections, acute respiratory infections, herpes infections) are hospitalized in this hospital. Diagnostic methods: polymerase chain reaction, ELISA, bacteriological.Results. Mixed infections are equally common in both boys and girls, while there is a predominance of mixed infections in young children (up to 1 year and from 1 year to 3 years) 62.4%. The structure of infectious morbidity is consistently dominated by acute enteric infection – 83.5% cases and acute respiratory infections – 53.0% cases. The progression of SARS-CoV-2-associated and herpes-associated mixed infections is characterized by a pronounced polymorphism of clinical manifestations. The etiological factor for each nosology was deciphered only in 17.7%; in 43.5% of cases, only one etiological factor was verified in the laboratory; in 38.8% of cases, no etiological factor was confirmed in the laboratory.
{"title":"Clinical and etiological characteristics of mixed infections in children in the Izhevsk hospital","authors":"T. A. Britkova, K. E. Panteleev, O. A. Pazinenko","doi":"10.22627/2072-8107-2022-21-4-53-56","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-53-56","url":null,"abstract":"Aim: to study the structure, features of the findings and treatment of mixed infections in children at the present stage.Materials and methods. 85 case histories of children aged 5 months to 17 years and 10 months were examined, the final diagnosis of which included 2 or more infections on the basis of the children's infectious diseases department City Clinical Hospital № 7 of Izhevsk. Children with acute infectious pathology (acute intestinal infections, acute respiratory infections, herpes infections) are hospitalized in this hospital. Diagnostic methods: polymerase chain reaction, ELISA, bacteriological.Results. Mixed infections are equally common in both boys and girls, while there is a predominance of mixed infections in young children (up to 1 year and from 1 year to 3 years) 62.4%. The structure of infectious morbidity is consistently dominated by acute enteric infection – 83.5% cases and acute respiratory infections – 53.0% cases. The progression of SARS-CoV-2-associated and herpes-associated mixed infections is characterized by a pronounced polymorphism of clinical manifestations. The etiological factor for each nosology was deciphered only in 17.7%; in 43.5% of cases, only one etiological factor was verified in the laboratory; in 38.8% of cases, no etiological factor was confirmed in the laboratory.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82261210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.22627/2072-8107-2022-21-4-43-48
T. M. Lebedeva, S. B. Chuelov, М. А. Sayfullin, A. L. Rossina, N. Zvereva, E. Pylaeva, G. Volkova, Yu. N. Ivanova, L. V. Kryukova, A. A. Litsev, L. Karan
The aim of the work is to summarize the available literature data on cases of visceral leishmaniasis in children registered in the Russian Federation, as well as to describe our own clinical observation of autochthonous visceral leishmaniasis in a 9-year-old child, in the diagnosis of which the polymerase chain reaction (PCR) method was used.Materials and methods: a review of domestic and foreign literature over the past 20 years, including Internet resources, was carried out. We observed a 9-year-old child hospitalized in the Russian Children's Clinical Hospital. Leishmania DNA was determined in blood serum by PCR using the Amplisens – Leishmania-Fl reagent kit.Results. It was established that the infection of the patient observed by us occurred on the territory of the Russian Federation in the Crimea. The diagnosis of leishmaniasis was confirmed by morphological examination of the bone marrow and the detection of pathogen DNA in the blood serum by PCR.Conclusion. On the territory of the Russian Federation there are foci of autochthonous visceral leishmaniasis in the Crimea and the North Caucasus. Polymerase chain reaction is an alternative to bone marrow aspirate microscopy for the diagnosis of visceral leishmaniasis.
{"title":"Autochthonous visceral leishmaniasis in the Russian Federation and the role of polymerase chain reaction as an alternative diagnostic method","authors":"T. M. Lebedeva, S. B. Chuelov, М. А. Sayfullin, A. L. Rossina, N. Zvereva, E. Pylaeva, G. Volkova, Yu. N. Ivanova, L. V. Kryukova, A. A. Litsev, L. Karan","doi":"10.22627/2072-8107-2022-21-4-43-48","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-43-48","url":null,"abstract":"The aim of the work is to summarize the available literature data on cases of visceral leishmaniasis in children registered in the Russian Federation, as well as to describe our own clinical observation of autochthonous visceral leishmaniasis in a 9-year-old child, in the diagnosis of which the polymerase chain reaction (PCR) method was used.Materials and methods: a review of domestic and foreign literature over the past 20 years, including Internet resources, was carried out. We observed a 9-year-old child hospitalized in the Russian Children's Clinical Hospital. Leishmania DNA was determined in blood serum by PCR using the Amplisens – Leishmania-Fl reagent kit.Results. It was established that the infection of the patient observed by us occurred on the territory of the Russian Federation in the Crimea. The diagnosis of leishmaniasis was confirmed by morphological examination of the bone marrow and the detection of pathogen DNA in the blood serum by PCR.Conclusion. On the territory of the Russian Federation there are foci of autochthonous visceral leishmaniasis in the Crimea and the North Caucasus. Polymerase chain reaction is an alternative to bone marrow aspirate microscopy for the diagnosis of visceral leishmaniasis.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85976208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.22627/2072-8107-2022-21-4-57-61
S. B. Chuelov, A. L. Rossina
The urgency of the problem of human alveolar and neotropic echinococcosis is due to polymorphism and the severity of clinical manifestations. The purpose and result of the work is to summarize the data available in the literature on the etiology, epidemiology, clinic, diagnosis, treatment, prevention of alveolar and neotropic echinococcosis in humans. Conclusion. The causative agent of human alveolar echinococcosis is E. multilocularis, neotropic – E. vogeli, E. oligarthra. The liver and lungs are most often affected, less often other organs of the abdominal cavity, bones, brain, spinal cord, eyes, etc. Diagnostics is carried out on the basis of a complex of clinical, instrumental, serological, molecular genetic studies. Treatment includes surgical removal of parasitic cysts and drug therapy.
{"title":"Alveolar and neotropical echinococcosis","authors":"S. B. Chuelov, A. L. Rossina","doi":"10.22627/2072-8107-2022-21-4-57-61","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-57-61","url":null,"abstract":"The urgency of the problem of human alveolar and neotropic echinococcosis is due to polymorphism and the severity of clinical manifestations. The purpose and result of the work is to summarize the data available in the literature on the etiology, epidemiology, clinic, diagnosis, treatment, prevention of alveolar and neotropic echinococcosis in humans. Conclusion. The causative agent of human alveolar echinococcosis is E. multilocularis, neotropic – E. vogeli, E. oligarthra. The liver and lungs are most often affected, less often other organs of the abdominal cavity, bones, brain, spinal cord, eyes, etc. Diagnostics is carried out on the basis of a complex of clinical, instrumental, serological, molecular genetic studies. Treatment includes surgical removal of parasitic cysts and drug therapy.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84512958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-24DOI: 10.22627/2072-8107-2022-21-4-49-52
L. U. Ulukhanova, N. S. Karnaeva, M. E. Dzhabrailova, S. G. Agaeva, A. G. Gadzhimirzaeva
The article shows the epidemiological situation of the coronavirus infection COVID-19 (SARS-CoV2) in the Republic of Dagestan for the period 2020-2022, according to the «Republican Center for Infectious Diseases and AIDS» of Makhachkala, Dagestan. Results. The epidemiological situation of the new coronavirus infection in the Republic of Dagestan (RD) remains unfavorable. In 2020, since the beginning of the pandemic, the «Wuhan» strain has been circulating, and since September 2020, the active circulation of the British «alpha» strain has begun. A new wave in 2021 with the highest incidence was recorded from June to September – associated with the inclusion of the Indian «delta» strain SARS-CoV-2 in the epidemic process. Strains of «omicron» that exist today are BA.1 and BA.2 may lead to hospitalization. They are still dangerous for residential people and patients with risk factors. Conclusion. Among the adult population, the largest number of cases of the disease was registered 90.7%. Three main age risk groups have been identified: the adult population from 50 to 64 years; from 65 years and older and from 40 to 49 years. The analysis of mortality by age groups also revealed the main risk groups: persons aged 50-59 years (15.12%); 60-64 years (23%) and over 65 years (55.6%). On 04.07.2022 1,557,661 people were vaccinated with the Gum-COVID-Vac, EpiVacCorona, KoviVak and Sputnik Light vaccine, including 1,377,645 people who completed vaccination.
{"title":"The epidemiological situation of coronavirus infection (COVID-19) in the Republic of Dagestan and measures to reduce it","authors":"L. U. Ulukhanova, N. S. Karnaeva, M. E. Dzhabrailova, S. G. Agaeva, A. G. Gadzhimirzaeva","doi":"10.22627/2072-8107-2022-21-4-49-52","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-49-52","url":null,"abstract":"The article shows the epidemiological situation of the coronavirus infection COVID-19 (SARS-CoV2) in the Republic of Dagestan for the period 2020-2022, according to the «Republican Center for Infectious Diseases and AIDS» of Makhachkala, Dagestan. Results. The epidemiological situation of the new coronavirus infection in the Republic of Dagestan (RD) remains unfavorable. In 2020, since the beginning of the pandemic, the «Wuhan» strain has been circulating, and since September 2020, the active circulation of the British «alpha» strain has begun. A new wave in 2021 with the highest incidence was recorded from June to September – associated with the inclusion of the Indian «delta» strain SARS-CoV-2 in the epidemic process. Strains of «omicron» that exist today are BA.1 and BA.2 may lead to hospitalization. They are still dangerous for residential people and patients with risk factors. Conclusion. Among the adult population, the largest number of cases of the disease was registered 90.7%. Three main age risk groups have been identified: the adult population from 50 to 64 years; from 65 years and older and from 40 to 49 years. The analysis of mortality by age groups also revealed the main risk groups: persons aged 50-59 years (15.12%); 60-64 years (23%) and over 65 years (55.6%). On 04.07.2022 1,557,661 people were vaccinated with the Gum-COVID-Vac, EpiVacCorona, KoviVak and Sputnik Light vaccine, including 1,377,645 people who completed vaccination.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89943236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.22627/2072-8107-2022-21-4-15-20
S. N. Eshmolov, E. G. Klimovitskaya, M. N. Kuzmina, I. Sitnikov
Diseases caused by herpesviruses are among the socially significant ones, which is determined by their wide distribution, polymorphism of clinical manifestations and severity of the course up to the development of lethal outcomes.The article presents literature data on the importance of the problem of lesions of the nervous system in herpesvirus infections at the present stage and the results of our own observations of 60 patients hospitalized in the Infectious Clinical Hospital of Yaroslavl in the period from 2015 to 2021. The analysis of the main clinical and laboratory indicators was carried out and the features of the course of lesions of the nervous system in herpesvirus infections were established.
{"title":"Lesions of the nervous system in herpesvirus infections","authors":"S. N. Eshmolov, E. G. Klimovitskaya, M. N. Kuzmina, I. Sitnikov","doi":"10.22627/2072-8107-2022-21-4-15-20","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-15-20","url":null,"abstract":"Diseases caused by herpesviruses are among the socially significant ones, which is determined by their wide distribution, polymorphism of clinical manifestations and severity of the course up to the development of lethal outcomes.The article presents literature data on the importance of the problem of lesions of the nervous system in herpesvirus infections at the present stage and the results of our own observations of 60 patients hospitalized in the Infectious Clinical Hospital of Yaroslavl in the period from 2015 to 2021. The analysis of the main clinical and laboratory indicators was carried out and the features of the course of lesions of the nervous system in herpesvirus infections were established.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79831131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.22627/2072-8107-2022-21-4-27-31
O. V. Molochkova, O. Kovalev, M. Kosyreva, N. Ilyina, O. Shamsheva, E. N. Getmanova, A. Korsunsky, E. Galeeva, A. A. Guzhavina
In Moscow, as in Russia, in 2019 there was an increase in the incidence of measles in recent years.Purpose: to analyze the course of measles in children hospitalized in 2019 during the period of rising incidence.Materials and methods: a retrospective analysis and statistical processing of 30 case histories of children hospitalized with measles in the infectious diseases department of the Children's City Clinical Hospital № 9 named after G.N. Speransky in 2019.Results: More than half of the patients were under the age of 3 years (53%). In 90% of cases, the sick were not vaccinated, in 73% – due to the refusal of their parents. Only 23% of patients had measles contact, mostly familial. Measles in hospitalized children proceeds typically, in 83.3% of cases in a moderate form, with characteristic pathognomonic symptoms: in 83.3% of patients, Belsky-Filatov-Koplik spots are detected, on average, on the 4.4 ± 0.8 day of illness, spotted – papular rash on the face with staged distribution and subsequent staged pigmentation in all patients. Complications develop in 66.7% of cases, more often pneumonia (36.7%) and obstructive bronchitis (20%), which in 16.7% of cases cause the severity of the course and lead to the development of emergency syndromes and conditions (respiratory failure, obstructive syndrome).Conclusions. Failure to vaccinate against measles contributes to morbidity with possible complications.
{"title":"Characteristics of measles in children during the rise in incidence in 2019","authors":"O. V. Molochkova, O. Kovalev, M. Kosyreva, N. Ilyina, O. Shamsheva, E. N. Getmanova, A. Korsunsky, E. Galeeva, A. A. Guzhavina","doi":"10.22627/2072-8107-2022-21-4-27-31","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-27-31","url":null,"abstract":"In Moscow, as in Russia, in 2019 there was an increase in the incidence of measles in recent years.Purpose: to analyze the course of measles in children hospitalized in 2019 during the period of rising incidence.Materials and methods: a retrospective analysis and statistical processing of 30 case histories of children hospitalized with measles in the infectious diseases department of the Children's City Clinical Hospital № 9 named after G.N. Speransky in 2019.Results: More than half of the patients were under the age of 3 years (53%). In 90% of cases, the sick were not vaccinated, in 73% – due to the refusal of their parents. Only 23% of patients had measles contact, mostly familial. Measles in hospitalized children proceeds typically, in 83.3% of cases in a moderate form, with characteristic pathognomonic symptoms: in 83.3% of patients, Belsky-Filatov-Koplik spots are detected, on average, on the 4.4 ± 0.8 day of illness, spotted – papular rash on the face with staged distribution and subsequent staged pigmentation in all patients. Complications develop in 66.7% of cases, more often pneumonia (36.7%) and obstructive bronchitis (20%), which in 16.7% of cases cause the severity of the course and lead to the development of emergency syndromes and conditions (respiratory failure, obstructive syndrome).Conclusions. Failure to vaccinate against measles contributes to morbidity with possible complications.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83586768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.22627/2072-8107-2022-21-4-32-36
R. F. Sayfullin, N. N. Zvereva, М. А. Saifullin, S. Smetanina, E. V. Kardonova, O. Shamsheva
The detection of antibodies to borrelia by enzyme immunoassay (ELISA) significantly depends on the time from the onset of the disease.Purpose: analysis of the results of antibodies determination to borrelia by ELISA in children and adults with Lyme borreliosis (LB) at various periods from the onset of the disease.Material and methods. We conducted a retrospective, non-randomized, single-center cohort study, based on the analysis of data from 178 outpatient records of patients with a confirmed diagnosis of LB and the presence of antibody detection results by ELISA. Immunological confirmation of the diagnosis of LB was carried out by using ELISA and western blot test systems registered in the territory of the Russian Federation for the separate determination of immunoglobulins to Borrelia burgdorferi antigens of classes M and G. Results. When counting from the date of the onset of the disease, IgM and/or IgG were determined in 76% of patients at 4-6 weeks, and starting from the 7th week – in 95%. When counting from the date of tick bite, IgG with or without IgM was determined in 83% of patients starting from 7th week. At the same time, a significantly large proportion of seronegative patients among children was revealed. We have clarified the duration of antibody persistence after antibacterial therapy. In the interval from 1 to 6 months, antibodies are detected in 73% of patients. For a period of 6 months or more, antibodies can be detected in 42% of patients.Conclusion. The optimal time for detecting antibodies from the disease onset is 4-6 weeks. Antibodies after antibiotic therapy can persist for a long time, in a third of patients up to 6 months or more.
{"title":"Detection of antibodies to B. burgdorferi by enzyme immunoassay in patients with Lyme borreliosis","authors":"R. F. Sayfullin, N. N. Zvereva, М. А. Saifullin, S. Smetanina, E. V. Kardonova, O. Shamsheva","doi":"10.22627/2072-8107-2022-21-4-32-36","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-32-36","url":null,"abstract":"The detection of antibodies to borrelia by enzyme immunoassay (ELISA) significantly depends on the time from the onset of the disease.Purpose: analysis of the results of antibodies determination to borrelia by ELISA in children and adults with Lyme borreliosis (LB) at various periods from the onset of the disease.Material and methods. We conducted a retrospective, non-randomized, single-center cohort study, based on the analysis of data from 178 outpatient records of patients with a confirmed diagnosis of LB and the presence of antibody detection results by ELISA. Immunological confirmation of the diagnosis of LB was carried out by using ELISA and western blot test systems registered in the territory of the Russian Federation for the separate determination of immunoglobulins to Borrelia burgdorferi antigens of classes M and G. Results. When counting from the date of the onset of the disease, IgM and/or IgG were determined in 76% of patients at 4-6 weeks, and starting from the 7th week – in 95%. When counting from the date of tick bite, IgG with or without IgM was determined in 83% of patients starting from 7th week. At the same time, a significantly large proportion of seronegative patients among children was revealed. We have clarified the duration of antibody persistence after antibacterial therapy. In the interval from 1 to 6 months, antibodies are detected in 73% of patients. For a period of 6 months or more, antibodies can be detected in 42% of patients.Conclusion. The optimal time for detecting antibodies from the disease onset is 4-6 weeks. Antibodies after antibiotic therapy can persist for a long time, in a third of patients up to 6 months or more.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80046623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.22627/2072-8107-2022-21-4-21-26
N. L. Pulnova, T. Rybalkina, N. Karazhas, R. E. Bosh’ian, M. Kornienko, O. F. Kabikova, N. Gabrielyan, I. Pashkova, O. Silina
Infections occupy one of the central places among the complications of transplants. The frequency of fatal infectious complications during the first twelve months after transplantation ranges from 2.6 to 51.7%. Identifying markers of opportunistic infections before transplantation will help reduce the likelihood of developing these infections after induced immunosuppression. The aim of the study was to study the role of herpesviruses and pneumocysts in the occurrence of infectious complications in children before and after liver transplantation based on the detection of markers of a number of herpesvirus infections and pneumocystosis. The article presents the results of a comprehensive examination for markers of herpesvirus infections and pneumocystosis of 70 children who were treated at the Shumakov Transplantation Research Center. It should be noted that 55 patients (78.6%) were diagnosed with infectious complications, of which 46 people (65.7%) had pneumonia. To detect IgM and IgG antibodies to herpesviruses and pneumocysts, peripheral blood serum samples were examined by enzyme immunoassay (ELISA). Detection of common HBV antigens was carried out by indirect immunofluorescence reaction (NRIF). Early antigens and reproduction of herpesviruses were detected by rapid culture method (BCM) on Vero and M-19 cell cultures for CMVI. In the event of infectious complications (pneumonia) in children who underwent related liver transplantation, the number of patients with active EBI markers increased almost 7 times and active HCV-6 markers increased 3.5 times. The paper shows the need for a broader laboratory screening of opportunistic infections, which will serve to achieve better clinical results, contribute to the formation of more advanced diagnostic algorithms, as well as improve epidemiological surveillance of these infections.
{"title":"The role of herpesviruses and pneumocysts in infectious complications in children during liver transplantation","authors":"N. L. Pulnova, T. Rybalkina, N. Karazhas, R. E. Bosh’ian, M. Kornienko, O. F. Kabikova, N. Gabrielyan, I. Pashkova, O. Silina","doi":"10.22627/2072-8107-2022-21-4-21-26","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-21-26","url":null,"abstract":"Infections occupy one of the central places among the complications of transplants. The frequency of fatal infectious complications during the first twelve months after transplantation ranges from 2.6 to 51.7%. Identifying markers of opportunistic infections before transplantation will help reduce the likelihood of developing these infections after induced immunosuppression. The aim of the study was to study the role of herpesviruses and pneumocysts in the occurrence of infectious complications in children before and after liver transplantation based on the detection of markers of a number of herpesvirus infections and pneumocystosis. The article presents the results of a comprehensive examination for markers of herpesvirus infections and pneumocystosis of 70 children who were treated at the Shumakov Transplantation Research Center. It should be noted that 55 patients (78.6%) were diagnosed with infectious complications, of which 46 people (65.7%) had pneumonia. To detect IgM and IgG antibodies to herpesviruses and pneumocysts, peripheral blood serum samples were examined by enzyme immunoassay (ELISA). Detection of common HBV antigens was carried out by indirect immunofluorescence reaction (NRIF). Early antigens and reproduction of herpesviruses were detected by rapid culture method (BCM) on Vero and M-19 cell cultures for CMVI. In the event of infectious complications (pneumonia) in children who underwent related liver transplantation, the number of patients with active EBI markers increased almost 7 times and active HCV-6 markers increased 3.5 times. The paper shows the need for a broader laboratory screening of opportunistic infections, which will serve to achieve better clinical results, contribute to the formation of more advanced diagnostic algorithms, as well as improve epidemiological surveillance of these infections.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74542220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.22627/2072-8107-2022-21-4-37-42
A. Medkova, E. G. Syomin, L. N. Sinyashina, I. Babachenko, G. I. Karataev
The aim of the study was to evaluate the effectiveness of the PCR-RТ method using the PCR-RТ-IS test system for the etiological diagnosis of whooping cough in the examination of patients with infectious respiratory pathology with prolonged cough and in contact persons. 113 children aged from 1 month to 17 years, 11 months and 29 days, and 146 contact family members were examined. Regardless of the initial diagnosis, all children were examined for whooping cough by bacteriological, molecular genetic and serological methods. For molecular genetic diagnostics a commercial kit and PCR-RT-IS test-system developed at the Gamaleya Research Center were used. The contact persons were examined by the bacteriological method and using the PCR-RT-IS test-system. During the initial examination the diagnoses «Acute respiratory viral infectious», «Acute rhinopharyngitis», «Acute laryngotracheitis», «Acute bronchitis», «Pneumonia» were established. The severity of cough in contact persons was different – from a typical spastic «whooping cough» to complete absence of cough. By using the PCR-RT-IS test-system we developed, the DNA of pertussis pathogen was detected in groups of patients with «Acute rhinopharyngitis» in 34.4% of cases, «Acute laryngotracheitis» – in 64.3%, «Acute bronchitis» – in 69%, with a diagnosis of «Pneumonia» – in 33.3% of cases. In a number of cases combined course of whooping cough with respiratory infections (respiratory syncytial virus, rhinovirus and others) was detected. In the examined contact persons the causative agent of pertussis was detected using the PCR-RT-IS test-system in 51,4% of cases.
{"title":"The effectiveness of PCR-RT method for etiological diagnosis of atypical forms of whooping cough","authors":"A. Medkova, E. G. Syomin, L. N. Sinyashina, I. Babachenko, G. I. Karataev","doi":"10.22627/2072-8107-2022-21-4-37-42","DOIUrl":"https://doi.org/10.22627/2072-8107-2022-21-4-37-42","url":null,"abstract":"The aim of the study was to evaluate the effectiveness of the PCR-RТ method using the PCR-RТ-IS test system for the etiological diagnosis of whooping cough in the examination of patients with infectious respiratory pathology with prolonged cough and in contact persons. 113 children aged from 1 month to 17 years, 11 months and 29 days, and 146 contact family members were examined. Regardless of the initial diagnosis, all children were examined for whooping cough by bacteriological, molecular genetic and serological methods. For molecular genetic diagnostics a commercial kit and PCR-RT-IS test-system developed at the Gamaleya Research Center were used. The contact persons were examined by the bacteriological method and using the PCR-RT-IS test-system. During the initial examination the diagnoses «Acute respiratory viral infectious», «Acute rhinopharyngitis», «Acute laryngotracheitis», «Acute bronchitis», «Pneumonia» were established. The severity of cough in contact persons was different – from a typical spastic «whooping cough» to complete absence of cough. By using the PCR-RT-IS test-system we developed, the DNA of pertussis pathogen was detected in groups of patients with «Acute rhinopharyngitis» in 34.4% of cases, «Acute laryngotracheitis» – in 64.3%, «Acute bronchitis» – in 69%, with a diagnosis of «Pneumonia» – in 33.3% of cases. In a number of cases combined course of whooping cough with respiratory infections (respiratory syncytial virus, rhinovirus and others) was detected. In the examined contact persons the causative agent of pertussis was detected using the PCR-RT-IS test-system in 51,4% of cases.","PeriodicalId":9855,"journal":{"name":"CHILDREN INFECTIONS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78644318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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