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Behaviour of short and long columns made from bamboo scrimber subjected to uniaxial compression 竹蔑制成的长短柱在单轴压缩下的行为
Pub Date : 2024-05-01 DOI: 10.1016/j.bamboo.2024.100082
Janeshka Goonewardena, Mahbube Subhani, Johannes Reiner, Bidur Kafle, Mahmud Ashraf

Engineered bamboo has been getting recognition as a forerunner in terms of desirable mechanical properties and a sustainable construction material. One of the most widespread variants is bamboo scrimber, also known as Parallel Bamboo Strand Lumber (PBSL). This paper presents a holistic investigation into the uniaxial compressive capacity of short bamboo scrimber columns in terms of failure mode, size effect, aspect ratio and failure strain. The studies in focus have been collated from published articles in literature. Furthermore, intermediately slender to long columns have been investigated with respect to the most common international timber standards and, elastic and inelastic theories. It is shown that the dominant failure mode affects the ultimate strength, especially a failure mode called kinking/local buckling. A size effect was observed and discussed with respect to the failure modes. In addition, a tangent modulus theory was proposed from a constitutive model called the Full-range Ramberg Osgood model that could better predict the critical buckling stress of intermediate-long columns over the conventional theory. Moreover, a modified AS1720 design approach was proposed to accommodate the inherent column behaviour of bamboo scrimber. Finally, a modified Newlin-Gahagan approach was proposed which produced consistent predictions for the experimental buckling stress for columns with varying slenderness across four different studies.

工程竹材作为具有理想机械性能和可持续建筑材料的先行者,已得到广泛认可。其中最常见的一种是竹节材,也被称为平行竹节材(PBSL)。本文从破坏模式、尺寸效应、长宽比和破坏应变等方面对短竹节材柱的单轴抗压能力进行了全面研究。重点研究内容来自已发表的文献文章。此外,还根据最常见的国际木材标准以及弹性和非弹性理论,对中间细长的柱子进行了研究。研究表明,主要失效模式会影响极限强度,尤其是一种称为扭结/局部屈曲的失效模式。在失效模式方面,观察到并讨论了尺寸效应。此外,根据全范围 Ramberg Osgood 模型提出了切线模量理论,与传统理论相比,该理论能更好地预测中长柱的临界屈曲应力。此外,还提出了修改后的 AS1720 设计方法,以适应竹护壁板固有的支柱行为。最后,提出了一种改进的纽林-加哈根方法,该方法在四项不同的研究中对不同细长度的柱子的实验屈曲应力做出了一致的预测。
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引用次数: 0
Efficacy of Pongamia pinnata seed oil as a fumigant in reducing surface infestation by sapstain and mould fungi in bamboo 松柏籽油作为熏蒸剂在减少竹子表面渍菌和霉菌侵染方面的功效
Pub Date : 2024-05-01 DOI: 10.1016/j.bamboo.2024.100085
Ajmal Samani , Biswajit Debnath, Akhato Sumi

Sap stain fungi causes discolouration in bamboo, which reduces the aesthetic value of bambooproducts and renders them unfit for any specific use in which appearance is a crucial factor in determining its value besides its durability. The efficacy of Pongamia pinnata seed oil (PSO) as a fumigant was tested against sapstain (Alternaria alternata) and mould (Aspergillus niger) fungi with a petri plate bioassay, observing growth inhibition by different concentrations of PSO. Growth inhibition ( %) was recorded as 65.8 % for 0.3 % PSO and 88.5 % for 0.5 % PSO.A 1.0 % PSO concentration showed almost complete (i.e. 98.8 %) growth inhibition in A. alternata. Growth inhibition of A. niger was 62.3 % for 0.3 % PSO, 92.6 % for 0.5 % PSO and 98.7 % for 1 % PSO compared to control samples. Surface coverage test by sapstain and mould fungi on 3 different bamboo species B tulda, Bambusa balcooa and Dendrocalamus strictus was also studied. Fumigation with PSO was effective in inhibiting the growth of sap stain and mould fungi in reducing surface infestation on all 3 bamboo species. Our results show that growth inhibition increased gradually with increasing PSO concentration and that complete inhibition in the growth of A. alternata and A. niger was observed in all bamboo species fumigated with a 15 % concentration of PSO.

渍菌会导致竹子褪色,从而降低竹制品的美学价值,使其不适合任何特定用途,而在这些用途中,外观是决定其价值的关键因素,此外还有耐用性。通过培养皿生物测定法测试了松柏籽油(PSO)作为熏蒸剂对吸浆虫(Alternaria alternata)和霉菌(Aspergillus niger)的功效,观察了不同浓度的 PSO 对生长的抑制作用。0.3 % PSO 和 0.5 % PSO 的生长抑制率分别为 65.8 % 和 88.5 %。与对照样品相比,0.3 % PSO 对 A. niger 的生长抑制率为 62.3 %,0.5 % PSO 为 92.6 %,1 % PSO 为 98.7 %。此外,还研究了三种不同竹子品种 B tulda、Bambusa balcooa 和 Dendrocalamus strictus 的汁液和霉菌表面覆盖试验。用 PSO 熏蒸能有效抑制渍渍菌和霉菌的生长,减少这三种竹子的表面侵染。我们的研究结果表明,随着 PSO 浓度的增加,生长抑制作用逐渐增强,在所有使用 15% 浓度 PSO 熏蒸的竹类中,均能完全抑制交替孢霉和黑曲霉的生长。
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引用次数: 0
Teaching students about sustainable construction dealing with bamboo building and trial investigations on mechanical properties of the bamboo culms 向学生传授有关竹建筑的可持续建筑知识,并对竹秆的机械性能进行试验性研究
Pub Date : 2024-05-01 DOI: 10.1016/j.bamboo.2024.100086
Pascal Franck , Oliver Bletz-Mühldorfer , Michael Weil , Leander Bathon , Andrés Bäppler , Sascha Luippold

In recent years the demand for sustainable materials in all technical fields has become increasingly urgent. This trend also applies to the building industry, which is striving for climate-friendly construction. Hence, the renewable and fast-growing material bamboo is a very promising solution. To bring bamboo closer to a potential application, a key interest is to teach the next generation of architects and civil engineers about the material, its properties and its behavior. Students from the RheinMain University of Applied Sciences in Wiesbaden, Germany, were invited to join lectures teaching them from conceptualization to the completion of a building made of bamboo. The aim was to create awareness and familiarity with this sustainable material for subsequent applications. Additionally, the students were given the chance to work with bamboo practically. For several months, trial investigations of the basic mechanical properties of bamboo culms were undertaken with the students. The experimental research included measurement of the moisture content, compression tests, and fractography. Knowledge about material properties and their determination should be brought to the students who are mostly unfamiliar with material testing. Based on the values measured during mechanical testing, the students should be able to do exemplary load-bearing calculations for the bamboo material.

近年来,各技术领域对可持续材料的需求日益迫切。这一趋势也同样适用于建筑行业,因为建筑行业正在努力实现气候友好型建筑。因此,可再生和快速生长的材料竹子是一个非常有前景的解决方案。为了使竹子更接近潜在的应用领域,关键是要向下一代建筑师和土木工程师传授有关这种材料、其特性和行为的知识。德国威斯巴登莱茵美茵应用科学大学的学生应邀参加了讲座,从竹子建筑的概念设计到竣工。这样做的目的是让学生们了解和熟悉这种可持续材料,以便日后应用。此外,学生们还有机会实际操作竹子。几个月来,学生们对竹秆的基本机械性能进行了试验性研究。实验研究包括含水量测量、压缩试验和断裂分析。由于学生大多不熟悉材料测试,因此应向他们传授有关材料特性及其测定的知识。根据机械测试中测得的数值,学生应能对竹材料进行示范性承重计算。
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引用次数: 0
Genome-wide identification, characterization, and expression pattern analysis of the JAZ gene family in Moso bamboo during rapid shoot development 毛竹快速发笋过程中 JAZ 基因家族的全基因组鉴定、特征描述和表达模式分析
Pub Date : 2024-05-01 DOI: 10.1016/j.bamboo.2024.100083
Xueyun Xuan , Shiying Su , Jiaqi Tan, Hui Guo, Yang Jiao, Zhijun Zhang

Moso bamboo (Phyllostachys heterocycla (Carriere) Matsum.), a member of the bamboo subfamily within the Gramineae family, is a globally significant economic forest resource, notable for its swift growth. JAZ proteins, crucial in the jasmonic acid signaling pathway, play essential roles in plant growth, development, and responses to various biotic and abiotic stresses. This study identifies 22 JAZ genes in moso bamboo using bioinformatics. We conducted a comprehensive analysis of their physicochemical properties, gene structure, chromosomal localization, conserved structural domains and motifs, cis-acting regulatory elements, and evolutionary relationships. Furthermore, by mining transcriptome data, we delineated the expression patterns of the JAZ gene family during the rapid shoot development phase in moso bamboo. This analysis suggests a significant role for the JAZ genes in the growth and development of moso bamboo. Additionally, we predicted transcription factors that regulate JAZ and analyzed protein interactions to construct an initial JAZ-related regulatory network. This study provides a crucial foundation for understanding the functions of the JAZ gene family and elucidating the molecular mechanisms underpinning the rapid shoot development in moso bamboo.

毛竹(Phyllostachys heterocycla (Carriere) Matsum.)是禾本科竹亚科的成员,是全球重要的经济林资源,以生长迅速而著称。JAZ 蛋白是茉莉酸信号通路中的关键蛋白,在植物的生长、发育以及对各种生物和非生物胁迫的反应中发挥着重要作用。本研究利用生物信息学方法鉴定了毛竹中的 22 个 JAZ 基因。我们对这些基因的理化性质、基因结构、染色体定位、保守结构域和基序、顺式作用调控元件以及进化关系进行了全面分析。此外,通过挖掘转录组数据,我们还描绘了毛竹快速发笋期 JAZ 基因家族的表达模式。这一分析表明,JAZ基因在毛竹的生长发育过程中发挥着重要作用。此外,我们还预测了调控JAZ的转录因子,并分析了蛋白质之间的相互作用,从而构建了一个初步的JAZ相关调控网络。这项研究为了解JAZ基因家族的功能和阐明毛竹快速发笋的分子机制奠定了重要基础。
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引用次数: 0
Comparison and assessment of nutritional composition in shoots of six bamboo species in Southeast China 中国东南六种竹笋营养成分的比较与评估
Pub Date : 2024-05-01 DOI: 10.1016/j.bamboo.2024.100087
Haibao Ji , Wanyu Ni , Ji Feng Shao

The introduction of bamboo species is one of the strategies used to enrich edible fresh bamboo shoots resources across diverse regions. However, there is a lack of investigation on the adaptability of bamboo species and the nutritional profiles of their shoots post-introduction. In this study, the nutritional components of bamboo shoots from six bamboo species that have been successfully introduced were compared and evaluated. These six bamboo species possess shoots rich in soluble sugar, soluble protein, and crude fibre. Furthermore, the shoots exhibit significant concentrations of potassium and iron, along with an abundance of amino acids. Notably, the amino acid content is highest in the apical region of the bamboo shoot, followed by the middle section, with the base having the lowest levels. When evaluated through several assessment systems, the shoots exhibited varying scores and rankings. A comprehensive evaluation approach was then utilized to assign final scores and rankings to all six species. Recommendations are provided for the selection of high-quality nutrient-dense bamboo shoots grown in the same field following successful introduction.

引进竹种是丰富不同地区可食用鲜笋资源的策略之一。然而,对竹种的适应性及其引进后笋的营养成分缺乏研究。在这项研究中,对已成功引进的六个竹种的竹笋营养成分进行了比较和评估。这六个竹种的笋含有丰富的可溶性糖、可溶性蛋白质和粗纤维。此外,笋中还含有大量的钾和铁,以及丰富的氨基酸。值得注意的是,氨基酸含量最高的是笋的顶端区域,其次是中段,基部含量最低。在通过多个评估系统进行评估时,笋的得分和排名各不相同。然后采用综合评估方法对所有六个物种进行最终评分和排名。为成功引进后在同一田地中种植的优质营养竹笋的选择提供了建议。
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引用次数: 0
Guadua guzmanii (Poaceae: Bambusoideae: Bambuseae: Guaduinae): A new woody bamboo species endemic to Jalisco, Mexico Guadua guzmanii (Poaceae: Bambusoideae: Bambuseae: Guaduinae):墨西哥哈利斯科州特有的木竹新种
Pub Date : 2024-05-01 DOI: 10.1016/j.bamboo.2024.100084
Eduardo Ruiz-Sanchez , María Del Carmen Anaya-Corona , Ximena Londoño

Guadua comprises 35 described species, seven of which are distributed in Mexico. Since 1976, a specimen corresponding to the genus Guadua was collected, which could not be correctly assigned to any described taxon. In the present study, a new species of Guadua endemic to the state of Jalisco is described based on vegetative characters. Herbarium specimens were reviewed, field collections made, and specialist literature consulted. A comparison with similar specimens was conducted. The degree of threat was determined following the criteria of the International Union for Conservation of Nature (IUCN). Guadua guzmanii, endemic to the El Tamarindo peninsula, municipality of La Huerta, Jalisco, is described. We include a morphological key for the species of the genus Guadua in Mexico, an illustration, photographs, and a distribution map. According to the IUCN criteria, the species is classified as Critically Endangered due to its restricted geographical distribution. With the description of Guadua guzmanii, the number of native Guadua species in Mexico increases to eight. Additionally, the number of woody bamboos in Mexico increases to 60, with 43 species endemic to Mexico and four endemic to Jalisco, included: Chusquea contrerasii, Chusquea guzmanii, Rhipidocladum singuliflorum, and Guadua guzmanii.

Guadua 有 35 个已描述的物种,其中 7 个分布在墨西哥。自 1976 年以来,人们收集到了一个与 Guadua 属相应的标本,但无法将其正确归入任何已描述的分类群。本研究根据植物特征描述了哈利斯科州特有的 Guadua 新种。研究人员查阅了标本馆标本,进行了野外采集,并查阅了专业文献。与类似标本进行了比较。根据世界自然保护联盟(IUCN)的标准确定了该物种的受威胁程度。本文描述了哈利斯科州拉韦尔塔市埃尔塔马林多半岛特有的 Guadua guzmanii。我们还提供了墨西哥 Guadua 属物种的形态学检索表、插图、照片和分布图。根据世界自然保护联盟(IUCN)的标准,该物种因地理分布有限而被列为极危物种。随着 Guadua guzmanii 的描述,墨西哥本地 Guadua 种类的数量增加到 8 种。此外,墨西哥的木本竹类增至 60 种,其中 43 种为墨西哥特有,4 种为哈利斯科州特有:Chusquea contrerasii、Chusquea guzmanii、Rhipidocladum singuliflorum 和 Guadua guzmanii。
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引用次数: 0
Dimensional stability and mechanical strength of thermally modified giant bamboo [Dendrocalamus asper (Schult.) Backer] using steam and oil 利用蒸汽和油对巨竹[Dendrocalamus asper (Schult.) Backer]进行热改性的尺寸稳定性和机械强度
Pub Date : 2024-04-21 DOI: 10.1016/j.bamboo.2024.100081
Juanito P. Jimenez Jr., James Edelbert C. Ramos

Various thermal modification (TM) treatments using different media have been commercialized, including the commonly practiced TM via steam and hot oil using vegetable or mineral oils. To expand the range of oil treatment mediums, this study explored the use of spent cooking oil (used oil from food frying in restaurants), which was centrally collected by a certified hauler and treater of this waste liquid. The effects on the dimensional stability and mechanical strength of giant bamboo [Dendrocalamus asper (Schult.) Backer] poles subjected to 175°C and 200°C for 30 min and 60 min in steam and oil, respectively were determined. The data were analyzed using a factorial experiment in a completely randomized design with TM treatments, temperature, duration, and presence of nodes as factors.

Results revealed significantly improved dimensional stability in modified bamboo as indicated by reduced moisture content, dimensional shrinkage/swelling, and water loss/absorption at higher temperatures. While treatment duration moderately affected dimensional stability, oil-treated samples exhibited greater enhancement.

In terms of mechanical strength, bamboo treated at 175°C exhibited enhanced flexural (modulus of rupture or MOR, and modulus of elasticity or MOE) and compressive strength (CS). However, treatments at 200°C led to marked reductions in these properties. Node presence generally weakened bamboo, except for CS. Oil-treated samples demonstrated superior mechanical strength, although there was a slight reduction in MOR with extended exposure.

In conclusion, steam and oil-based TM improve the dimensional stability and mechanical strength of giant bamboo at 175°C. For optimal properties, a 30-min treatment is recommended. This research underscores the viability of spent cooking oil in enhancing bamboo's physico-mechanical attributes, contributing to sustainable applications.

使用不同介质的各种热改性(TM)处理方法已经商业化,其中包括通过蒸汽和热油(使用植物油或矿物油)进行热改性的常用方法。为了扩大油处理介质的范围,本研究探索了使用废食用油(餐馆煎炸食物的废油)的方法,这种废油由一家经过认证的废液运输和处理商集中收集。研究测定了在蒸汽和油中分别经受 175°C 和 200°C 30 分钟和 60 分钟对巨竹(Dendrocalamus asper (Schult.) Backer)竹竿的尺寸稳定性和机械强度的影响。结果表明,在较高温度下,改性竹材的尺寸稳定性显著提高,这表现在含水率、尺寸收缩/膨胀和失水/吸水率降低。就机械强度而言,在 175°C 下处理的竹子的抗弯强度(断裂模数或 MOR 和弹性模量或 MOE)和抗压强度(CS)都有所提高。然而,在 200°C 高温下处理竹材会明显降低这些性能。除 CS 外,节点的存在通常会削弱竹材的强度。总之,蒸汽和油基热处理可提高巨竹在 175°C 下的尺寸稳定性和机械强度。为了获得最佳性能,建议进行 30 分钟的处理。这项研究强调了废食用油在提高竹子物理机械属性方面的可行性,有助于实现可持续应用。
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引用次数: 0
Bamboo structures: Innovative methods and applications for structural health monitoring and dissemination 竹结构:结构健康监测和传播的创新方法与应用
Pub Date : 2024-04-18 DOI: 10.1016/j.bamboo.2024.100079
Vincenzo Barrile, Emanuela Genovese

Bamboo is gaining more and more attention in the field of building materials thanks to its unique characteristics, such as resistance, lightness, sustainability, and flexibility. However, the widespread adoption of bamboo as a building material presents some challenges, especially in relation to the durability of the material. In fact, being a natural material, it requires protection from atmospheric agents, such as rain and sun, as well as from attacks by insects and animals. Ensuring the longevity of bamboo involves implementing tailored treatments, precise precautions, and regular structural inspections to detect any potential degradation over time. In this context, the article focused on the monitoring of a structure made of bamboo and on the testing of an innovative system to identify the structural critical issues present, in the context of a FISR project which envisaged a cultural exchange between China and Italy. Starting from the survey of the structure carried out with a drone, a 3D model was then built using a commercial software. For the monitoring phase of the structure, an innovative system was used that exploits the YOLO v5s6 algorithm to identify structural critical issues, supported by a Virtual/Augmented/Mixed reality app developed by the authors. This app is useful not only during the monitoring phase, but also in the subsequent phase of disseminating information relating to the potential of the use of bamboo in the field of structural engineering. The tested and proposed methodologies have proven to be particularly useful and high-performance, especially regarding the monitoring of bamboo structures, in relation to the resolution of problems linked to their possible fragility and limitations in certain application areas. The contribution provided in this paper by Geomatics methodologies is evident to better highlight the potential of this eco-friendly material for its desirable ever-increasing use in the construction sector by becoming aware of its potential.

竹子因其独特的特性,如耐磨、轻便、可持续性和灵活性,在建筑材料领域正受到越来越多的关注。然而,竹子作为建筑材料的广泛应用也带来了一些挑战,尤其是在材料的耐久性方面。事实上,作为一种天然材料,竹子需要免受雨水和阳光等大气介质的侵蚀,以及昆虫和动物的攻击。要确保竹子的使用寿命,就需要对竹子进行有针对性的处理,采取精确的预防措施,并定期进行结构检查,以发现竹子随着时间的推移可能出现的退化。在此背景下,文章重点介绍了对竹子结构的监测,以及对一个创新系统的测试,以确定在中意两国文化交流项目中存在的结构性关键问题。从使用无人机对结构进行勘测开始,使用商业软件建立了三维模型。在结构监测阶段,使用了一个创新系统,该系统利用 YOLO v5s6 算法识别结构关键问题,并由作者开发的虚拟/增强/混合现实应用程序提供支持。该应用程序不仅在监测阶段非常有用,而且在随后的结构工程领域竹材使用潜力信息传播阶段也非常有用。事实证明,经过测试和建议的方法是非常有用和高效的,特别是在竹结构监测方面,可以解决竹结构在某些应用领域可能存在的脆弱性和局限性问题。本文通过地理信息学方法所做的贡献是显而易见的,即通过意识到这种生态友好材料的潜力,更好地突出其在建筑领域日益增长的应用。
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引用次数: 0
Application of silvicultural treatments to improve local Gigantochloa apus (Schult. & Schult.f.) Kurz ex Munro stands for supporting handicraft industries in Gunungkidul region, Indonesia 应用造林处理方法改善印度尼西亚 Gunungkidul 地区当地的 Gigantochloa apus (Schult. & Schult.f.) Kurz ex Munro 林分,为手工艺产业提供支持
Pub Date : 2024-04-12 DOI: 10.1016/j.bamboo.2024.100076
Levina Augusta Geraldine Pieter , Aris Sudomo , Suhartono , Ary Widiyanto , Marcellinus Mandira Budi Utomo

There is a growing demand within the Gunungkidul region of Indonesia for bamboo, particularly Gigantochloa apus (Schult.f.) Kurz ex Munro, which is used in handicrafts. This study examined ways to enhance the productivity of bamboo to meet the specific requirements of the craft industry in the region. It assessed the impact of three levels of thinning intensity and three types of fertilizer application on the productivity and quality of G. apus. The type of fertilizer application significantly influenced the diameter of the sixth internode and the overall height of the bamboo while having an insignificant effect on the number of bamboo shoots, internode diameter at one meter or internode length. Thinning intensity significantly affected several aspects, including the number of shoots, the diameter of the sixth internode and the overall height of the bamboo. Clump thinning intensity increased the diameter of the remaining bamboo clump but reduced the number of shoots, as well as internode length and overall height. The interaction between thinning intensity and fertilizer application significantly impacted the diameter of bamboo, the diameter of the sixth internode, and the overall height of bamboo, without significant effects on the number of shoots or the internode length.

印度尼西亚古农基杜尔地区对竹子的需求日益增长,尤其是对用于手工艺品的竹子 Gigantochloa apus (Schult.f.) Kurz ex Munro。这项研究探讨了如何提高竹子的生产力,以满足该地区手工艺品行业的特殊要求。研究评估了三种疏伐强度和三种施肥类型对楠竹产量和质量的影响。施肥类型对第六节节间直径和竹子整体高度有明显影响,而对竹笋数量、一米节间直径或节间长度的影响不明显。疏伐强度对竹笋数量、第六节节间直径和竹子总高度等几个方面都有明显影响。竹丛疏伐强度增加了剩余竹丛的直径,但减少了竹笋数量、节间长度和总高度。疏伐强度与施肥量之间的交互作用对竹子的直径、第六节节间直径和竹子的总高度有显著影响,而对竹笋的数量和节间长度没有显著影响。
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引用次数: 0
Response surface optimization for deep eutectic solvents extraction of bamboo shoots proteins 响应面优化深共晶溶剂提取竹笋蛋白质的方法
Pub Date : 2024-04-09 DOI: 10.1016/j.bamboo.2024.100080
Siyi Wang, Qifan Liu, Chang Xu, Yuan Gao, Xuejun Yu

To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of Chimonobambusa purpurea Hsueh f. & T.P.Yi (syn. Chimonobambusa neopurpurea T.P.Yi) bamboo shoots. Choline chloride and proline were utilized as hydrogen bond acceptors in a total of eight deep eutectic solvents, whereas glucose, sucrose, glycerol, ethylene glycol, acetylpropionic acid, malic acid, and lactic acid were used as hydrogen bond donors. This work examined the protein extraction from bamboo shoots using deep eutectic solvents. Single-factor experiments were conducted to determine the optimal composition of the deep eutectic solvents, along with the ideal gradients of material-liquid ratio gradients, water content, extraction time, and water bath temperature. The impact and interplay of various variables on the protein extraction rate of bamboo shoots were examined using the Box-Behnken Design and Response Surface Methodology. Both the water bath temperature and the extraction time significantly influenced the protein extraction rate of bamboo shoots. Notably, the interaction between extraction time and water bath temperature had the most substantial effect on the protein extraction rate of bamboo shoots. The quadratic response surface regression equation for the protein extraction rate of bamboo shoots relative to the actual values of each factor was obtained as follows: Protein extraction rate = −193.3275 + 3.8395 A - 18.7075 B + 6.3285 C + 0.2125 AB - 0.0089 AC + 0.024 BC - 0.02885 A2 + 0.685 B2 - 0.06455 C2. Finally, this work predicted optimum extraction parameters for bamboo shoots with choline chloride-sucrose (molar ratio 1:2), a material-liquid ratio of 1:50, a water content of 74 %, an extraction time of 4 h, and a water bath temperature of 44 ℃. Under the optimal conditions, the protein extraction yield was 59.39±0.45 %, which aligned with the predicted value from the response surface fitting equation. This indicated that the optimised regression equation may have practical significance. Compared with conventional extraction using a sodium hydroxide (NaOH) solution, deep eutectic solvents extraction of bamboo shoots is more environmentally friendly, non-toxic and harmless. It provides a theoretical and experimental reference for industrial extraction and offers a reference for deep eutectic solvent development in bamboo shoot resource utilization.

Key message:

To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of bamboo shoots.

为了提高竹笋蛋白质的提取率,采用响应面法对竹笋蛋白质的提取工艺进行了优化。在总共八种深共晶溶剂中,氯化胆碱和脯氨酸被用作氢键受体,而葡萄糖、蔗糖、甘油、乙二醇、乙酰丙酸、苹果酸和乳酸被用作氢键供体。这项研究考察了使用深共晶溶剂从竹笋中提取蛋白质的过程。通过单因素实验确定了深共晶溶剂的最佳成分,以及理想的料液比梯度、含水量、萃取时间和水浴温度。采用箱-贝肯设计法和响应面法研究了各种变量对竹笋蛋白质提取率的影响和相互作用。水浴温度和提取时间都对竹笋蛋白质提取率有显著影响。值得注意的是,提取时间与水浴温度之间的交互作用对竹笋蛋白质提取率的影响最大。竹笋蛋白质提取率相对于各因素实际值的二次响应面回归方程为蛋白质提取率 = -193.3275 + 3.8395 A - 18.7075 B + 6.3285 C + 0.2125 AB - 0.0089 AC + 0.024 BC - 0.02885 A2 + 0.685 B2 - 0.06455 C2。最后,本研究预测了氯化胆碱-蔗糖(摩尔比为 1:2)、料液比为 1:50、含水量为 74%、提取时间为 4 h、水浴温度为 44 ℃的竹笋最佳提取参数。在最佳条件下,蛋白质提取率为 59.39±0.45%,与响应面拟合方程的预测值一致。这表明优化回归方程可能具有实际意义。与传统的氢氧化钠(NaOH)溶液萃取相比,深层共晶溶剂萃取竹笋更环保、无毒、无害。主要信息:为提高竹笋蛋白质提取率,采用响应面法对竹笋蛋白质提取工艺进行了优化。
{"title":"Response surface optimization for deep eutectic solvents extraction of bamboo shoots proteins","authors":"Siyi Wang,&nbsp;Qifan Liu,&nbsp;Chang Xu,&nbsp;Yuan Gao,&nbsp;Xuejun Yu","doi":"10.1016/j.bamboo.2024.100080","DOIUrl":"https://doi.org/10.1016/j.bamboo.2024.100080","url":null,"abstract":"<div><p>To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of <em>Chimonobambusa purpurea</em> Hsueh f. &amp; T.P.Yi (syn. <em>Chimonobambusa neopurpurea</em> T.P.Yi) bamboo shoots. Choline chloride and proline were utilized as hydrogen bond acceptors in a total of eight deep eutectic solvents, whereas glucose, sucrose, glycerol, ethylene glycol, acetylpropionic acid, malic acid, and lactic acid were used as hydrogen bond donors. This work examined the protein extraction from bamboo shoots using deep eutectic solvents. Single-factor experiments were conducted to determine the optimal composition of the deep eutectic solvents, along with the ideal gradients of material-liquid ratio gradients, water content, extraction time, and water bath temperature. The impact and interplay of various variables on the protein extraction rate of bamboo shoots were examined using the Box-Behnken Design and Response Surface Methodology. Both the water bath temperature and the extraction time significantly influenced the protein extraction rate of bamboo shoots. Notably, the interaction between extraction time and water bath temperature had the most substantial effect on the protein extraction rate of bamboo shoots. The quadratic response surface regression equation for the protein extraction rate of bamboo shoots relative to the actual values of each factor was obtained as follows: Protein extraction rate = −193.3275 + 3.8395 A - 18.7075 B + 6.3285 C + 0.2125 AB - 0.0089 AC + 0.024 BC - 0.02885 A<sup>2</sup> + 0.685 B<sup>2</sup> - 0.06455 C<sup>2</sup>. Finally, this work predicted optimum extraction parameters for bamboo shoots with choline chloride-sucrose (molar ratio 1:2), a material-liquid ratio of 1:50, a water content of 74 %, an extraction time of 4 h, and a water bath temperature of 44 ℃. Under the optimal conditions, the protein extraction yield was 59.39±0.45 %, which aligned with the predicted value from the response surface fitting equation. This indicated that the optimised regression equation may have practical significance. Compared with conventional extraction using a sodium hydroxide (NaOH) solution, deep eutectic solvents extraction of bamboo shoots is more environmentally friendly, non-toxic and harmless. It provides a theoretical and experimental reference for industrial extraction and offers a reference for deep eutectic solvent development in bamboo shoot resource utilization.</p><p>Key message:</p><p>To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of bamboo shoots.</p></div>","PeriodicalId":100040,"journal":{"name":"Advances in Bamboo Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773139124000259/pdfft?md5=c39ccc5ed0081909a7593df0bfd3f661&pid=1-s2.0-S2773139124000259-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140548609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Advances in Bamboo Science
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