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Determinates and governance of bamboo production and marketing in Ethiopia: A critical review 埃塞俄比亚竹子生产和营销的决定因素和治理:一个批判性的回顾
Pub Date : 2023-10-16 DOI: 10.1016/j.bamboo.2023.100047
Asabeneh Alemayehu , Alemayehu Hido

Oldeania alpina (highland) and Oxytenanthera abyssinica (lowland) bamboo are two indigenous bamboo species and are vigorous in highland and lowland agro-ecologies, respectively. They have enormous socioeconomic, ecological, and traditional roles in the community. We address bamboo plantations and their distribution, identify factors influencing bamboo planting and marketing, and assess the role of bamboo for smallholder farmers and customers in Ethiopia. We also examine planting and management practices, governance, and challenges and opportunities for bamboo production and marketing. The decisions to plant bamboo are influenced by many demographic and socioeconomic factors, such as age, educational status, family size, income, farm size, land scarcity, bamboo culm price, and market information. They are also influenced by institutional factors, including market distance, marketing linkages, access to markets, tenure security, and psychological factors such as the perception of risk and attitude towards bamboo production. Bamboo plays a variety of socio-economic, ecological, and traditional roles in the community. It is one of the essential components of agricultural tasks and practices for most households and provides considerable cash income. For instance, it provides material for house and fence construction, furniture, wood fuel, and musical instruments. It is used as a source of food, medicine, and fodder and also for soil and water conservation, biodiversity functioning, and mitigating global climate change, as well as an alternative source of timber and non-timber products for rural and urban people, forest industries, construction, and energy sectors. Despite this, its significance has been affected by deforestation and degradation, overexploitation, increased agricultural investment, and social issues. Although there is a positive policy environment, there is still a lack of coordination, overlap of roles and responsibilities, and limited dedicated manpower and resources in the bamboo sector, as well as limited government support for the value chain development of the sector. Creating an enabling market environment and constructing a value chain are critical for the development of the sector. Furthermore, the selection of higher-quality bamboo landraces, extension support, and the local application of the established regulations are all critical.

高山Oldeania alpina(高地)和低地Oxytenthera abyssinica(低地)竹是两个本土竹种,分别在高地和低地农业生态中生长旺盛。他们在社区中扮演着巨大的社会经济、生态和传统角色。我们关注竹子种植园及其分布,确定影响竹子种植和营销的因素,并评估竹子对埃塞俄比亚小农户和客户的作用。我们还研究了竹子生产和营销的种植和管理实践、治理以及挑战和机遇。种植竹子的决定受到许多人口和社会经济因素的影响,如年龄、教育状况、家庭规模、收入、农场规模、土地稀缺性、竹竿价格和市场信息。它们还受到体制因素的影响,包括市场距离、营销联系、市场准入、保有权保障,以及心理因素,如对风险的感知和对竹子生产的态度。竹子在社区中扮演着各种社会经济、生态和传统角色。它是大多数家庭农业任务和做法的重要组成部分之一,并提供可观的现金收入。例如,它为房屋和围栏的建造、家具、木材燃料和乐器提供材料。它被用作食物、药品和饲料的来源,也用于水土保持、生物多样性运作和缓解全球气候变化,以及农村和城市人民、林业、建筑业和能源部门的木材和非木材产品的替代来源。尽管如此,其重要性仍受到森林砍伐和退化、过度开发、农业投资增加和社会问题的影响。尽管存在积极的政策环境,但竹子行业仍然缺乏协调,角色和责任重叠,专门的人力和资源有限,政府对该行业价值链发展的支持有限。创造有利的市场环境和构建价值链对该行业的发展至关重要。此外,选择更高质量的竹子品种、推广支持以及在当地实施既定法规都至关重要。
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引用次数: 0
Durability and strength sustainability assessment of rockwool-bamboo reinforced ceiling boards 岩棉竹增强天花板的耐久性和强度可持续性评估
Pub Date : 2023-10-08 DOI: 10.1016/j.bamboo.2023.100046
Olumoyewa D. Atoyebi , Oluwafemi O. Kolajo , Oluwasegun J. Aladegboye , Olayinka O. Alabi , Salome O. Aina

It is increasingly important to explore means of reducing deforestation in our environment by sourcing alternatives for timber in the forest. Bamboo (Bambusa vulgaris) is known for its strength and flexibility, which makes it a popular material for construction, furniture, and other uses that are still being explored. This paper evaluated the use of bamboo for the design of ceiling boards based on the physical and mechanical properties of bamboo. We explored the use of bamboo powder, bamboo fibre, and bamboo sticks that are usually discarded during construction activities, using urea-formaldehyde as a binding agent. The bamboo ceiling boards were tested for their physical (water absorption, thickness swelling, and density) and mechanical (modulus of rupture and modulus of elasticity) properties. Water absorption ranged from 41.9% to 86.4% after 2 h immersion in water and from 41.9% to 97.6% after 24hrs immersion. The modulus of rupture was 78.915 N/mm2 and the highest density was 0.424 g/cm3. Given these results, bamboo is a suitable material for the production of ceiling boards.

通过寻找森林中木材的替代品来探索减少环境中森林砍伐的方法越来越重要。竹子(Bambusa vulgaris)以其强度和灵活性而闻名,这使其成为建筑、家具和其他仍在探索中的用途的流行材料。本文根据竹子的物理力学性能,对竹子在天花板设计中的应用进行了评价。我们探索了使用建筑活动中通常丢弃的竹粉、竹纤维和竹签,使用脲醛作为粘合剂。测试了竹天花板的物理性能(吸水性、厚度膨胀性和密度)和机械性能(断裂模量和弹性模量)。在水中浸泡2小时后,吸水率为41.9%至86.4%,在浸泡24小时后吸水率为4.19%至97.6%。断裂模量为78.915N/mm2,最高密度为0.424g/cm3。鉴于这些结果,竹子是生产天花板的合适材料。
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引用次数: 0
Advances in engineered bamboo processing: Material conversion and structure 工程竹加工的进展:材料转化与结构
Pub Date : 2023-09-26 DOI: 10.1016/j.bamboo.2023.100045
Mohammad Khajouei-Nezhad , Kate Semple , Vahid Nasir , Yu’an Hu , Greg Marggraf , Jonas Hauptman , Chunping Dai

With bamboo’s rapid renewability, short rotation period, versatility, and good mechanical properties, engineered bamboo composites have undergone significant evolution. This paper examines the recent changes in bamboo product manufacturing, starting with a wholistic classification of culm breakdown to the plethora of different constituent elements: full culm, flattened bamboo, bamboo bundles, splits/strips, mats/curtains, and strands. Highly cracked flattened strips used in bamboo scrimber are 4–5 times greater in specific surface area than crack-free strips, requiring an estimated 6–10 times higher resin consumption than laminated bamboo. Based on their structure, bamboo composites can be classified into three categories: 1) lamination of minimally modified culms, 2) laminated mat structures with controlled or semi-random placement of constituent elements and 3) random mat structures composed of thin bamboo strands. Composite formation is transitioning from random placement of large, thick strips to more controlled layup with secondary elements of stitched strip curtains, helping minimize edge-to-edge strip overlaps and improve bonding efficiency with less mat compaction. Density-structure-strength property relationships are also compared between various engineered bamboo and common engineered wood products. Other technical advancements include crack-free flattened bamboo and hybrid bamboo-wood products.

由于竹子可再生性快、旋转周期短、多功能性强、力学性能好,工程竹复合材料发生了重大变化。本文考察了竹制品制造业的最新变化,从竹秆的整体分类开始,将其分解为过多的不同组成元素:全秆、扁竹、竹束、劈叉/条、席/帘和股。竹纤维中使用的高度开裂的扁平条的比表面积是无裂纹条的4-5倍,所需的树脂消耗量估计是层压竹的6-10倍。根据其结构,竹复合材料可分为三类:1)最小改性秆的层压,2)具有可控或半随机放置组成元素的层压垫结构,以及3)由细竹股组成的随机垫结构。复合材料的形成正从大而厚的板条的随机放置过渡到与缝合板条帘的次要元素的更可控的叠层,有助于最大限度地减少边到边的板条重叠,并通过较少的垫压实提高粘合效率。比较了各种工程竹与普通工程木制品的密度结构强度性能关系。其他技术进步包括无裂纹的扁平竹子和竹木混合产品。
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引用次数: 0
Assessment of the drying behaviour of young and mature Bambusa vulgaris from Ghana 加纳青壮年斑竹干燥行为的评价
Pub Date : 2023-09-12 DOI: 10.1016/j.bamboo.2023.100044
Michael Awotwe-Mensah , Stephen Jobson Mitchual , Emmanuel Appiah-Kubi , Peter Kessels Dadzie , Prosper Mensah , Mark Bright Donkor

This study assessed the drying behaviour of young and mature Bambusa vulgaris culms and evaluated their utilisation potential. Two and four-year old Bambusa vulgaris culms were harvested and prepared to the required length of 21 internodes. The first seven internodes were taken as the bottom, the second seven were taken as middle and the last seven internodes were taken as the top. The study used samples from the bottom and top. Physical properties, including basic density and moisture content, were determined in accordance with ISO 13061–2 (ISO 2014) and EN 13183–1 (EN 2002), respectively, while shrinkage properties (radial, tangential, longitudinal and volumetric) were determined following established procedures. For the bottom and top portions of the mature bamboo after 18 days of drying, radial shrinkage values were 3.31% and 5.01%, tangential values were 4.95% and 5.78%, and longitudinal values were 0.28% and 0.35%, respectively. For the bottom and top portion of two-year-old culms, the values were 15.0% and 18.6% for radial shrinkage, 20.5% and 25.8% for tangential shrinkage and 0.46% and 0.47% for longitudinal shrinkage, respectively. The tangential shrinkage values were higher than the radial and longitudinal shrinkage values. The moisture content of the bottom part of the mature bamboo culm after 3 and 18 days of drying were 31.4% and 12.2%, while the young culms had values of 53.5% and 27.8%, respectively. Mature bamboo culms had better drying behaviour than young culms. Consequently, mature bamboo culms are more suitable for use in engineered composite materials.

本研究评估了幼年和成熟斑竹秆的干燥行为,并评估了其利用潜力。收获2年和4年生的斑竹秆,并将其制备至21个节间的所需长度。前7个节间为底部,后7个节间居中,最后7个节间顶部。这项研究使用了底部和顶部的样本。物理性能,包括基本密度和含水量,分别根据ISO 13061-2(ISO 2014)和EN 13183-1(EN 2002)进行测定,而收缩性能(径向、切向、纵向和体积)则根据既定程序进行测定。干燥18天后,成熟竹子的底部和顶部的径向收缩值分别为3.31%和5.01%,切向收缩值为4.95%和5.78%,纵向收缩值为0.28%和0.35%。对于两年生秆的底部和顶部,径向收缩的值分别为15.0%和18.6%,切向收缩的值为20.5%和25.8%,纵向收缩的值则分别为0.46%和0.47%。切向收缩值高于径向和纵向收缩值。干燥3天和18天后,成熟竹秆底部的水分含量分别为31.4%和12.2%,而年轻竹秆的水分含量值分别为53.5%和27.8%。成熟的竹秆比年轻的竹秆具有更好的干燥性能。因此,成熟的竹竿更适合用于工程复合材料。
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引用次数: 1
Determinants of bamboo processors' utilization level: The case of Bahir Dar city and Injibara town, Ethiopia 竹加工企业利用水平的决定因素——以埃塞俄比亚Bahir Dar市和Injibara镇为例
Pub Date : 2023-09-11 DOI: 10.1016/j.bamboo.2023.100043
Tsadiku Setegne Dessie, Ahmed Mohammed Yimer, Mohammed Yimam Ali

Bamboo is an important non-timber forest resource with multiple uses across many different sectors. It has many economic, social, and ecological roles and is seen as a key to reducing poverty in some regions because of its value to local communities and industry. Bamboo is a plentiful resource in Ethiopia, although it is underutilized. The primary goal of this study was to investigate the factors that influence the utilization of bamboo by processors. A questionnaire survey was used to investigate the effects of the awareness levels of processors, market demand, financial capacity, technical accessibility, supporting markets, and market linkages on the levels of utilization by processors, as measured by the variety of bamboo products. The data were collected from bamboo processors using a questionnaire administered by trained data collectors in Bahir Dar and Injibara and were analysed using ordinal logistic regression with the SPSS 20 software package. Access to technology, supporting markets, processors' awareness, and financial capacity were statistically significant determinants that positively affected the utilization of bamboo, but market demand and market linkages were not. We recommend that government at all levels provide policy-based support and attention, either financially or in other ways, and that responsible agencies such as Technique, Vocational and Enterprise Development Centres, Micro and Small Enterprise Offices, Trading and Agricultural Bureaus cooperating with the federal government, and the International Network of Bamboo and Rattan (INBAR) should prepare manuals, videos, leaflets, skill-oriented training, and experience-sharing programmes. Future research should expand on the current study by including more variables on expanded targets and using other models that consider the interaction effects of independent variables. They should also conduct comparative studies of bamboo utilization levels among different bamboo processors in different regions of Ethiopia.

竹子是一种重要的非木材森林资源,在许多不同部门具有多种用途。它具有许多经济、社会和生态作用,由于其对当地社区和工业的价值,被视为一些地区减少贫困的关键。竹子在埃塞俄比亚是一种丰富的资源,尽管它没有得到充分利用。本研究的主要目的是调查影响加工商利用竹子的因素。通过问卷调查,调查了加工商的意识水平、市场需求、财务能力、技术可及性、支持市场和市场联系对加工商利用水平的影响,以竹产品的种类为衡量标准。数据由Bahir Dar和Injibara训练有素的数据采集员使用问卷从竹子加工厂收集,并使用SPSS 20软件包使用有序逻辑回归进行分析。获得技术、支持市场、加工商的意识和财政能力是对竹子利用产生积极影响的统计上重要的决定因素,但市场需求和市场联系不是。我们建议各级政府以财政或其他方式提供基于政策的支持和关注,并建议技术、职业和企业发展中心、微型和小型企业办公室、贸易和农业局等负责机构与联邦政府合作,国际竹藤网应编写手册、视频、传单、技能培训和经验交流方案。未来的研究应该在当前研究的基础上进行扩展,在扩展的目标上加入更多的变量,并使用其他考虑自变量相互作用影响的模型。他们还应对埃塞俄比亚不同地区不同竹子加工商的竹子利用水平进行比较研究。
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引用次数: 0
Physical, mechanical, chemical, and durability assessment of water leaching treatment of bamboo 竹子水浸处理的物理、机械、化学和耐久性评估
Pub Date : 2023-08-09 DOI: 10.1016/j.bamboo.2023.100040
Leo Maia do Amaral , Marzieh Kadivar , Juarez Benigno Paes , Djeison Cesar Batista , Miquéias de Souza Reis , Abasalt Tarverdi , André Luiz Pereira de Godoy Jr , Holmer Savastano Jr

Water leaching is a traditional bamboo treatment method in which fresh bamboo poles are immersed in running water for at least 30 days to leach out water-soluble substances such as starch and carbohydrates. This method is easy to apply and does not require additional chemicals. Although water leaching is widely applied, its influence on the physical, mechanical, and chemical properties of bamboo and its effect on the durability of bamboo against termites has not been fully explored through experimental methods. This paper assesses the effects of water leaching treatment on bamboo and identifies the potential and limitations of this treatment. A 23% reduction in the density of bamboo after water treatment was found, which can be related to the removal of water-soluble extractives from the material. A significant (15%) increase in the modulus of rupture and a 20% increase in the modulus of elasticity were identified using a three-point bending test after water treatment while keeping a similar fibre volume fraction. Water leached bamboo had less dimensional stability and higher water absorption than untreated bamboo. As the density of the bamboo decreased, more space became available in its bundles for water. A darkened surface was observed after treatment, but no chemical modifications were found through FT-IR spectroscopy. Termites can use cellulose as a source of food, and the removal of starch from bamboo did not improve its resistance against this insect. On the contrary, the free space created after treatment favoured the action of the termites, leading to a higher mass loss percentage for treated bamboo. The water leaching system should not be used as the only treatment method in bamboo being utilized for structural purposes, and the reduction of dimensional stability and the darkened surface must be considered when using the bamboo for furniture and handicraft applications.

水浸是一种传统的竹子处理方法,将新鲜的竹竿浸泡在自来水中至少30天,以浸出淀粉和碳水化合物等水溶性物质。这种方法易于应用,不需要额外的化学物质。尽管水浸法应用广泛,但其对竹子物理、机械和化学性能的影响以及对竹子抗白蚁耐久性的影响尚未通过实验方法得到充分探讨。本文评估了水浸处理对竹子的影响,并确定了这种处理的潜力和局限性。发现水处理后竹子的密度降低了23%,这可能与从材料中去除水溶性提取物有关。水处理后,在保持类似纤维体积分数的同时,使用三点弯曲试验确定断裂模量显著增加(15%),弹性模量增加20%。与未经处理的竹子相比,水浸竹子的尺寸稳定性较差,吸水率较高。随着竹子密度的降低,竹子捆中有更多的空间可以用来蓄水。处理后观察到变暗的表面,但通过FT-IR光谱没有发现化学修饰。白蚁可以使用纤维素作为食物来源,从竹子中去除淀粉并不能提高其对这种昆虫的抵抗力。相反,处理后产生的自由空间有利于白蚁的活动,导致处理后的竹子质量损失率更高。水浸出系统不应被用作用于结构目的的竹子的唯一处理方法,并且在将竹子用于家具和工艺品应用时,必须考虑尺寸稳定性的降低和表面变暗。
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引用次数: 0
Influence of the outer skin on the flexural properties and thermal conductivity of densified Dendrocalamus asper bamboo 表皮对致密化麻竹弯曲性能和导热性能的影响
Pub Date : 2023-08-09 DOI: 10.1016/j.bamboo.2023.100041
A.D. de Sá , M. Kadivar , G.H.A. Barbirato , A. Tarverdi , S. Kadivar , L.M. do Amaral , H. Savastano Júnior

In this work, the effect of densification and the maintenance of the outer skin on the flexural properties and thermal conductivity of Dendrocalamus asper Backer ex K.Heyne bamboo was investigated. The results showed improvements in bending resistance for the densified samples compared to the un-densified bamboo. Densified samples with the outer skin showed the highest bending strength. Samples with the outer culm wall in compression offered the best results, with an average modulus of rupture (MOR) of around 348.9 MPa and modulus of elasticity (MOE) of 28.4 GPa. These values are around 40% and 86% (MOR), and 17% and 30% (MOE) higher compared to densified samples without the skin and un-densified samples, respectively. The fracture sections of specimens after bending tests were analyzed using Scanning Electron Microscopy (SEM) to visualize crack propagation within the outer skin and bamboo tissues. Maintaining the skin increases the thermal conductivity, and the densified bamboo with the skin has a thermal conductivity of around 0.23 W/m.K, 12.8% higher than the densified bamboo without skin and 22.5% higher than un-densified bamboo. Although a reduction in thermal performance was achieved, it was concluded that is reasonable to keep the bamboo skin, as it improves the overall mechanical resistance and reduces material loss during bamboo processing.

本工作研究了致密化和外皮维护对松竹弯曲性能和热导率的影响。结果表明,与未致密的竹子相比,致密样品的抗弯性有所提高。具有外皮的致密样品显示出最高的弯曲强度。具有压缩外秆壁的样品提供了最好的结果,平均断裂模量(MOR)约为348.9MPa,弹性模量(MOE)为28.4GPa。与没有表皮和未致密样品的致密样品相比,这些值分别高出约40%和86%(MOR,17%和30%)。使用扫描电子显微镜(SEM)分析弯曲试验后试样的断裂截面,以观察裂纹在外皮和竹组织内的扩展。保持表皮增加了热导率,有表皮的致密竹子的热导率约为0.23W/m.K,比没有表皮的致密竹高12.8%,比未致密竹高22.5%。尽管热性能有所降低,但得出的结论是,保留竹皮是合理的,因为它提高了整体机械阻力,并减少了竹子加工过程中的材料损失。
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引用次数: 0
Lowland bamboo (Oxytenanthera abyssinica) deforestation and subsequent cultivation effects on soil physico-chemical properties in northwestern Ethiopia 埃塞俄比亚西北部低地竹的砍伐及其栽培对土壤理化性质的影响
Pub Date : 2023-08-01 DOI: 10.1016/j.bamboo.2023.100038
Zebene Tadesse , Melkamu Abere , Belayneh Azene , Pan Kaiwen , Yigardu Mulatu , Meta Francis

In Ethiopia, bamboo thickets and woodlands play an important role in soil-water conservation and climate change mitigation in arid and semi-arid regions. However, bamboo mass flowering, rapid demographic changes and expansion of agricultural investments into bamboo dominated areas have led to deforestation and land degradation. This study assessed the effects of deforestation and subsequent cultivation on soil physical and chemical properties along a chronosequence of closely located agricultural lands with different times (1, 3, 5 and 7 years) since conversion from natural lowland bamboo forest. Soil samples (n = 90) were taken from both natural bamboo forests and adjacent agricultural lands at two soil depths (0–20 cm and 20–40 cm). Cation exchange capacity (CEC), K+, Ca2+, Mg2+ and available P varied significantly among cultivation periods and soil depth, while soil pH and Na+ varied with soil depth (P < 0.001). Soil C and total N contents (g/kg) in the 0–20 cm soil layer declined significantly and exponentially with increasing years under cultivation. Conversion of natural bamboo forest to cropland during the past seven-year period significantly increased soil pH with soil depth, while CEC declined throughout the cultivation period and with soil depth. In general, the results revealed that conversion of natural lowland bamboo forest and subsequent cultivation of the soil had negative effects on the measured soil physico-chemical properties.

在埃塞俄比亚,竹林和林地在干旱和半干旱地区的水土保持和减缓气候变化方面发挥着重要作用。然而,竹子大量开花、人口结构的快速变化以及农业投资向以竹子为主的地区的扩张,导致了森林砍伐和土地退化。本研究评估了从天然低地竹林转换以来不同时间(1年、3年、5年和7年)的森林砍伐和随后的耕作对土壤物理和化学性质的影响。土壤样本(n=90)取自两个土壤深度(0–20 cm和20–40 cm)的天然竹林和邻近农田。阳离子交换容量(CEC)、K+、Ca2+、Mg2+和有效磷随种植期和土壤深度的不同而显著变化,而土壤pH和Na+随土壤深度的变化而变化(P<;0.001)。0–20cm土层中的土壤C和总N含量(g/kg)随种植年限的增加呈指数级显著下降。在过去的七年里,天然竹林退耕还林显著增加了土壤pH值,而CEC在整个种植期和土壤深度内都有所下降。总的来说,研究结果表明,天然低地竹林的改造和随后的土壤耕作对测量的土壤理化性质有负面影响。
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引用次数: 0
Fimbribambusa jokowii Widjaja (Poaceae: Bambusoideae), a new scrambling bamboo from Flores, Indonesia 印度尼西亚弗洛雷斯的一种新的扰序竹Fimbribambusa jokowii Widjaja
Pub Date : 2023-08-01 DOI: 10.1016/j.bamboo.2023.100033
Elizabeth A. Widjaja

A new bamboo species, quite widely distributed along the border between Sikka and East Flores Districts on Flores, Indonesia, is described and illustrated. It is named in honour of Ir Joko Widodo, the President of Indonesia. The main distinction between this and other Fimbribambusa Widjaja species is the culm sheath auricle, which is long, glabrous and black, the linear sheath blade on the culms, the yellowish base to the leaves, the small leaf sheath auricle and long bristle, and the entire ligule.

描述并举例说明了一种新的竹子物种,该物种广泛分布在印度尼西亚弗洛雷斯的锡卡区和东弗洛雷斯区之间的边界上。它是为了纪念印度尼西亚总统伊尔·佐科·维多多而命名的。该品种与其他Fimbribambusa Widjaja物种的主要区别是秆鞘耳廓,其长、无毛且黑色,秆上的线形鞘叶片,叶片的黄色基部,小叶鞘耳廓和长刚毛,以及整个叶舌。
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引用次数: 0
Effect of light spectra on in vitro multiplication, elongation and adventitious rooting stages of Bambusa vulgaris Schrad. ex J. C. Wendl. 光谱对斑竹离体增殖、伸长和不定根阶段的影响。前J.C.温德尔。
Pub Date : 2023-08-01 DOI: 10.1016/j.bamboo.2023.100035
Douglas Santos Gonçalves , Denys Matheus Santana Costa Souza , Sérgio Bruno Fernandes , Letícia Vaz Molinari , Adriano Francis Dorigan , Enéas Ricardo Konzen , Gustavo Leal Teixeira , Gilvano Ebling Brondani

Bamboos occur throughout much of the temperate and tropical world, have rapid growth, and have various commercial and environmental applications. Clonal production of selected plants on a industrial scale is an important strategy for the bamboo sector. This study aimed to evaluate the effects of the light spectrum on in vitro multiplication, elongation, adventitious rooting, and anatomical features of the leaf surface of Bambusa vulgaris. In the multiplication and elongation stages, in vitro–established explants were transferred to a culture medium supplemented with 8.88 µmol of 6-benzylaminopurine (BAP) and 2.69 µmol of α-naphthalene acetic acid (NAA), and subjected to four light spectra (i.e., white, blue, green, and red). At the adventitious rooting stage, the culture medium was supplemented with 9.84 µmol of indole-3-butyric acid (IBA), 5.37 µmol NAA, and 2.22 µmol BAP under identical light spectra. Explant survival was not influenced by light spectra in the multiplication and elongation stages. White (2.2 shoots) and blue (1.8 shoots) light spectra were the most suitable for the number of shoots per explant. The white spectrum was associated with the highest average length of shoots (7.4 cm) and number of leaves per explant (3.0 leaves). The white light spectrum resulted in the highest average chlorophyll a contents (12.60 µg mg−1), total chlorophyll (16.60 µg mg−1), and carotenoids (10.10 µg mg−1). White and blue light spectra resulted in the best responses for vigor, and least senescence and tissue oxidation. White and blue light spectra favored the chlorophyll b content, resulting in 4.60 and 3.60 µg mg−1, respectively. Survival (80.0 %), adventitious rooting (50.0 %), vigor, senescence, and tissue oxidation were favored in the white light spectrum in the adventitious rooting stage. Scanning electron microscopy of leaves exposed to the white light spectrum revealed microtrichomes and spines on the adaxial surface of the leaf blade, papillae and stomata; on the abaxial surface, there were many unicellular trichomes arranged in rows, denoting normal growth and development. These results may help the production of micropropagated plants of Bambusa vulgaris on an industrial scale.

竹子分布在温带和热带世界的大部分地区,生长迅速,具有各种商业和环境应用。在工业规模上克隆生产选定的植物是竹子行业的一项重要战略。本研究旨在评价光谱对斑竹离体增殖、伸长、不定根和叶片表面解剖特征的影响。在增殖和伸长阶段,将体外建立的外植体转移到补充有8.88µmol 6-苄基氨基嘌呤(BAP)和2.69µmolα-萘乙酸(NAA)的培养基中,并进行四种光谱(即白色、蓝色、绿色和红色)。在不定根阶段,在相同的光谱下,培养基中添加9.84µmol吲哚-3-丁酸(IBA)、5.37µmol NAA和2.22µmol BAP。在增殖和伸长阶段,外植体的存活不受光谱的影响。白色(2.2芽)和蓝色(1.8芽)的光谱最适合每个外植体的芽数。白色光谱与最高的平均芽长(7.4厘米)和每个外植体的叶片数(3.0片)有关。白光光谱的平均叶绿素a含量最高(12.60µg mg−1),总叶绿素含量最高(16.60µg g mg−2),类胡萝卜素含量最高(10.10µg mg–1)。白光和蓝光光谱的活力反应最好,衰老和组织氧化最少。白色和蓝色光谱有利于叶绿素b含量,分别为4.60和3.60µg mg−1。在不定根阶段的白光光谱中,存活率(80.0%)、不定根率(50.0%)、活力、衰老和组织氧化是有利的。对暴露在白光光谱下的叶片进行扫描电子显微镜检查,发现叶片、乳头和气孔的正轴表面有微毛体和棘;背面有许多排列成行的单细胞毛状体,表明生长发育正常。这些结果可能有助于在工业规模上生产小斑竹的微繁殖植物。
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Advances in Bamboo Science
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