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Obesity and oxidative stress: Implications for female fertility 肥胖与氧化应激:对女性生育能力的影响
Pub Date : 2024-09-13 DOI: 10.1002/aro2.82
Nuo Heng, Huabin Zhu, Anup Kumar Talukder, Shanjiang Zhao

Obesity has reached epidemic proportions in most parts of the world, and it is estimated that 1 billion people globally are trapped in an obesity pandemic, which has seriously compromised human health. Recently, there has been a flood of research into obesity as well as redox and lipid metabolism; however, our understanding of the pathways and specific molecular mechanisms by which obesity-induced oxidative stress affects female reproductive function remains limited. In this review, we discuss how the obesity pandemic has led to lower female fertility. We focus on multiple facets of obesity-mediated reproductive dysfunction, including follicular atresia, oocyte maturation, embryo implantation, reproductive aging, and discuss therapeutic interventions that have the potential to normalize reproductive function in obese females, such as targeting mitochondrial lipid metabolism and antioxidant pathways.

肥胖症在世界大部分地区已达到流行病的程度,据估计,全球有 10 亿人陷入肥胖症的流行之中,严重损害了人类的健康。最近,有关肥胖以及氧化还原和脂质代谢的研究如雨后春笋般涌现;然而,我们对肥胖诱导的氧化应激影响女性生殖功能的途径和具体分子机制的了解仍然有限。在本综述中,我们将讨论肥胖大流行是如何导致女性生育力下降的。我们关注肥胖介导的生殖功能障碍的多个方面,包括卵泡闭锁、卵母细胞成熟、胚胎植入、生殖衰老,并讨论了有可能使肥胖女性生殖功能正常化的治疗干预措施,如针对线粒体脂质代谢和抗氧化途径的干预措施。
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引用次数: 0
Review of One Welfare by Rebeca García Pinillos
Pub Date : 2024-09-05 DOI: 10.1002/aro2.78
Donald M. Broom
<p>Attitudes to the position of humans in the world, the status of nonhuman animals, and ideas about animal welfare have developed rapidly in recent years. This is partly because of a change in world economics, as consumers now have more power and dictate what they will buy, and partly because of advances in knowledge about the biology of all animals, including humans and the increasing damage to the world that is the result of human action [<span>1</span>].</p><p>The economic change is from a push economy, driven largely by producers, to a pull economy, driven largely by consumers. Consumers now wish to avoid products and actions that they consider to be morally wrong. More and more they are refusing to buy anything that is not sustainably produced. A system or procedure is sustainable if it is acceptable now and if its expected future effects are acceptable, particularly in relation to resource availability, consequences of functioning, and morality of action [<span>2</span>]. Modern consumers require transparency in commercial and government activities and take account of the ethics of production when they evaluate product quality. There are many components of sustainability and all should be taken into account but, for food products, many consumers in countries where surveys have been conducted consider the welfare of animals used in or affected by production to be a very important part of sustainability. For example, consumers are refusing to buy pig meat if the sows have been kept in stalls or tethers, eggs or egg products from hens in battery cages, or crop products if many bees, birds, or people have died as a result of herbicide or pesticide use.</p><p>The concepts of one biology, one health, and one welfare emphasize that each of these words mean the same whether the subject of the biology, health, or welfare is human or nonhuman [<span>3, 4</span>]. Moral action is not just about humans but concerns all sentient animals, and to some extent, all living beings. In the introduction to her book, Rebeca Garcia Pinillos emphasizes these points and says “One Welfare is a concept that encompasses, in a multidimensional way, a number of areas, including: the problems of animal production, workers and the impact on the environment of livestock farms; the reduction of violence across the world; and the connection between poor animal and human welfare states and how improved animal welfare can help improve human welfare.” Monique Eloit, Director General of the World Organization for Animal Health (OIE), in a foreword to this book, has emphasized that the book develops further the “now well accepted” concept of One Health and “with the adoption of the OIE Global Animal Welfare Strategy during the 85th OIE General Session in May 2017, the OIE has specifically acknowledged the importance of multi-stakeholder and interdisciplinary approaches to animal welfare, and integrated this into our work”.</p><p>The extent to which scientific studies of welfare
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引用次数: 0
Partially substituting alfalfa hay with hemp forage promotes the health and well-being of goats via altering ruminal and plasma metabolites and metabolic pathways 用大麻饲草部分替代苜蓿干草,通过改变瘤胃和血浆代谢物及代谢途径促进山羊的健康和福祉
Pub Date : 2024-08-28 DOI: 10.1002/aro2.77
Tao Ran, Guowang Luo, Yipeng Yue, Zhipeng Xu, Zunji Shi, Zhaomin Lei, Wenzhu Yang, Duanqin Wu

Hemp forage (HF) seems a suitable forage for ruminants for its high nutritional value and rich phytochemicals that exert health and growth-promoting activities. We investigated the effects of hemp-related phytochemicals on rumen and plasma metabolism using metabolome when partially substituting alfalfa hay with HF in goat diets. Numbers of differential metabolites linearly increased with increasing HF substituting rate, approximately 50% of which were phytochemicals. Metabolic pathway enrichment analysis showed that the inclusion of HF greatly promoted steroid hormone biosynthesis, one carbon pool by folate, and retinol metabolism pathways in both rumen and plasma, which are beneficial for promoting animal health and well-being and enhancing the quality of animal products. Some phytochemicals showed inhibitory activities on the growth of certain ruminal bacteria; meanwhile, the detected intermediate metabolites indicated degradation of the phytochemicals by ruminal microbes. These phytochemicals work individually and synergistically to alter ruminal and plasma metabolic pathways, thus exerting benefits in promoting the health and well-being of animals.

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引用次数: 0
CircDYRK1A regulates bovine myoblasts development by binding miR21-5p to affect KLF5 gene expression CircDYRK1A 通过结合 miR21-5p 影响 KLF5 基因的表达来调控牛肌母细胞的发育
Pub Date : 2024-08-21 DOI: 10.1002/aro2.76
Peng Yang, Xinmiao Li, Lei Du, Shijie Lyu, Zijing Zhang, Fengpeng Lin, Xinglei Qi, Xian Liu, Eryao Wang, Chuzhao Lei, Yongzhen Huang

Circular RNA (circRNA), a stable ring-shaped RNA molecule found in eukaryotic cells, plays significant roles in biological regulation, particularly by interfering with transcription factor binding or enhancing gene expression. Using transcriptomic sequencing, we identified differentially expressed circRNAs in bovine muscle at various time points. Specifically, circDYRK1A was discovered and shown to enhance differentiation while suppressing proliferation of adult myoblasts. Rescue experiments further demonstrated that circDYRK1A regulates the KLF5 gene expression by interacting with miR21-5p, thus exerting its influence at the transcriptional level. This study marks the first identification of circDYRK1A in cattle and elucidates its role in bovine myoblast development through the circDYRK1A-miR21-5p-KLF5 regulatory axis. These findings contribute novel insights into molecular breeding of cattle and advance fundamental research on beef cattle breeding and muscle development.

环状 RNA(circRNA)是真核细胞中一种稳定的环状 RNA 分子,在生物调控中发挥着重要作用,特别是通过干扰转录因子结合或增强基因表达。通过转录组测序,我们发现了不同时间点牛肌肉中表达不同的 circRNA。具体来说,我们发现了 circDYRK1A,并证明它能促进成肌细胞的分化,同时抑制其增殖。拯救实验进一步证明,circDYRK1A 通过与 miR21-5p 相互作用来调节 KLF5 基因的表达,从而在转录水平发挥影响。这项研究标志着首次在牛体内鉴定出 circDYRK1A,并阐明了它通过 circDYRK1A-miR21-5p-KLF5 调控轴在牛肌细胞发育中的作用。这些发现为牛的分子育种提供了新的见解,推动了肉牛育种和肌肉发育的基础研究。
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引用次数: 0
Temperature and humidity as drivers for the transmission of zoonotic diseases 温度和湿度是人畜共患疾病传播的驱动因素
Pub Date : 2024-08-03 DOI: 10.1002/aro2.75
Li Zhang, Chenrui Lv, Wenqiang Guo, Zhenzhuo Li

Zoonotic diseases remain a persistent threat to global public health. Many major zoonotic pathogens exhibit seasonal patterns associated with climatic variations. Quantifying the impacts of environmental variables such as temperature and humidity on disease transmission dynamics is critical for improving prediction and control measures. This review synthesizes current evidence on the relationships between temperature and humidity and major zoonotic diseases, including malaria, dengue, rabies, anisakiasis, and influenza. Overall, this review highlighted some overarching themes across the different zoonotic diseases examined. Higher temperatures within suitable ranges were generally associated with increased transmission risks, while excessively high or low temperatures had adverse effects. Humidity exhibited complex nonlinear relationships, facilitating transmission in certain temperature zones but inhibiting it in others. Heavy rainfall and high humidity were linked to vector-borne disease outbreaks such as malaria by enabling vector breeding. However, reduced incidence of some diseases like dengue fever was observed with high rainfall. To address existing knowledge gaps, future research efforts should prioritize several key areas: enhancing data quality through robust surveillance and the integration of high-resolution microclimate data; standardizing analytical frameworks and leveraging advanced methodologies such as machine learning; conducting mechanistic studies to elucidate pathogen, vector, and host responses to climatic stimuli; adopting interdisciplinary approaches to account for interacting drivers; and projecting disease impacts under various climate change scenarios to inform adaptation strategies. Investing in these research priorities can propel the development of evidence-based climate-aware disease prediction and control measures, ultimately safeguarding public health more effectively.

人畜共患病仍然是全球公共卫生的一个长期威胁。许多主要的人畜共患病原体表现出与气候变异相关的季节性模式。量化温度和湿度等环境变量对疾病传播动态的影响对于改进预测和控制措施至关重要。本综述综述了温度和湿度与疟疾、登革热、狂犬病、疟原虫病和流感等主要人畜共患病之间关系的现有证据。总体而言,本综述强调了所研究的不同人畜共患病的一些总体主题。在适宜的温度范围内,较高的温度通常会增加传播风险,而过高或过低的温度则会产生不利影响。湿度表现出复杂的非线性关系,在某些温度区域会促进传播,但在其他区域则会抑制传播。暴雨和高湿度有利于病媒繁殖,从而与疟疾等病媒传播疾病的爆发有关。然而,在降雨量大的情况下,登革热等一些疾病的发病率也有所降低。为了弥补现有的知识差距,未来的研究工作应优先考虑以下几个关键领域:通过强有力的监测和整合高分辨率微气候数据来提高数据质量;实现分析框架标准化并利用机器学习等先进方法;开展机理研究以阐明病原体、病媒和宿主对气候刺激的反应;采用跨学科方法来考虑相互作用的驱动因素;以及预测各种气候变化情景下的疾病影响,为适应战略提供信息。对这些研究重点进行投资,可以推动开发以证据为基础、具有气候意识的疾病预测和控制措施,最终更有效地保障公众健康。
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引用次数: 0
Free iron accumulation and oxidative stress burden induce ferroptotic atrophy of chicken yolk sac during the late embryogenesis 游离铁积累和氧化应激负担诱导鸡卵黄囊在胚胎后期发生铁性萎缩
Pub Date : 2024-07-08 DOI: 10.1002/aro2.74
Huichao Liu, Zehe Song, Xi He, Haihan Zhang

The aim of this study was to investigate the mechanism of iron homeostasis and the ferroptosis pathway for yolk sac atrophy during late embryogenesis. To study the mechanism of yolk sac atrophy, 100 eggs were used. Further, 500 eggs were randomly divided into five treatments and in ovo feeding with different iron sources, such as FeSO4, ferrous glycinate (Fe-Gly), or deferoxamine (DFO), to study the effects of free iron content on hatching quality and embryonic development. The results showed that total iron content of yolk decreased, but yolk sac increased from embryonic(E)13 to E19 (p < 0.05). Comparison of gene expression of iron transport systems showed that free iron accumulation and dysfunction occurred in the yolk sac. Yolk sac metabolites at E19 compared to E13 were more enriched in histidine and sulfur pathways, suppressing glutathione synthesis and resulting in oxidative stress damage in the yolk sac. Combined analysis of differential metabolites and gene expression in ferroptosis pathway at E13 and E19 revealed the activation of the yolk sac during late embryogenesis was probably through up-regulation of ACSL4 expression and down-regulation of GPX4 expression. Furthermore, in ovo feeding FeSO4 shortened the incubation time compared to CON, while Fe-Gly or DFO delayed the hatching peak and increased hatching weight with less residual yolk. Collectively, it can be concluded that yolk sac atrophy during late embryogenesis may be mediated by iron disorders and provides a novel insight to modulate yolk sac nutrition, and hatching efficiency in chickens.

本研究旨在探究胚胎发育后期卵黄囊萎缩的铁平衡机制和铁变态反应途径。为研究卵黄囊萎缩的机制,使用了 100 枚鸡蛋。然后,将500枚鸡蛋随机分为5个处理,分别用不同的铁源(如FeSO4、甘氨酸亚铁(Fe-Gly)或去铁胺(DFO))进行卵喂养,研究游离铁含量对孵化质量和胚胎发育的影响。结果表明,从胚胎(E)13到E19,卵黄中的总铁含量下降,但卵黄囊的总铁含量上升(p < 0.05)。铁运输系统基因表达的比较表明,卵黄囊中出现了游离铁的积累和功能障碍。与E13相比,E19期卵黄囊代谢物中组氨酸和硫的含量更高,抑制了谷胱甘肽的合成,导致卵黄囊氧化应激损伤。综合分析E13和E19期铁硫代谢物和基因表达的差异,发现胚胎后期卵黄囊的活化可能是通过上调ACSL4的表达和下调GPX4的表达实现的。此外,与CON相比,FeSO4缩短了孵化时间,而Fe-Gly或DFO推迟了孵化高峰,增加了孵化重量,减少了卵黄残留。综上所述,胚胎后期卵黄囊萎缩可能是由铁失调介导的,这为调节卵黄囊营养和鸡的孵化效率提供了新的视角。
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引用次数: 0
Selection of the most informative wavenumbers to improve prediction accuracy of milk fatty acid profile based on milk mid-infrared spectra data 根据牛奶中红外光谱数据选择信息量最大的波长,提高牛奶脂肪酸谱预测的准确性
Pub Date : 2024-07-08 DOI: 10.1002/aro2.72
Wenqi Lou, Luiz F. Brito, Xiuxin Zhao, Valentina Bonfatti, Jianbin Li, Yachun Wang

Milk mid-infrared (MIR) spectra have been shown to provide valuable information on a wide range of traits to be used in dairy cattle breeding programs. Selecting the most informative variables from complex data can improve the prediction accuracy and model robustness and, consequently, the interpretability of MIR spectra. Thus, we aimed to investigate the prediction performance of feature selection methods based on MIR spectra data, using the milk fatty acid (FA) profile as an example to illustrate the evaluated procedure. Data of MIR spectra, milk test-day records, and reference FA concentrations of 155 first-parity Holstein cows were used in the analyses. Four models comprising different explanatory variables and three feature selection methods were evaluated. The results indicated that competitive adaptive reweighted sampling (CARS) method can effectively select the most informative variables from the MIR spectra, resulting in higher prediction accuracies than other variable selection approaches. The model including selected MIR spectra and cow information variables yielded the best FA profile predictions based on partial least square regression. C8:0, C10:0, C14:1, C17:0 isomers, C18:1, C18:1 isomer, medium-chain FA, unsaturation FA, monounsaturated FA, and polyunsaturated FA presented accuracies based on the determination coefficient ranging from 0.66 to 0.85 in internal validation and from 0.65 to 0.84 in external validation. The most related wavenumbers to 35 FAs were found within 1003 to 1145 cm−1. Generally, using CARS and cow information improved predictions of FAs based on MIR spectra in Chinese Holstein dairy cows. Additional validation studies should be conducted as larger datasets become available.

牛奶中红外光谱(MIR)已被证明可为奶牛育种计划提供大量有价值的性状信息。从复杂数据中选择信息量最大的变量可以提高预测精度和模型稳健性,从而提高中红外光谱的可解释性。因此,我们旨在研究基于近红外光谱数据的特征选择方法的预测性能,并以牛奶脂肪酸(FA)曲线为例说明评估程序。分析中使用了 155 头头等荷斯坦奶牛的近红外光谱数据、牛奶测试日记录和参考脂肪酸浓度。评估了由不同解释变量和三种特征选择方法组成的四个模型。结果表明,竞争性自适应加权采样(CARS)方法能有效地从近红外光谱中选择信息量最大的变量,因此预测准确率高于其他变量选择方法。根据偏最小二乘法回归,包含所选近红外光谱和奶牛信息变量的模型可获得最佳的 FA 轮廓预测结果。在内部验证和外部验证中,C8:0、C10:0、C14:1、C17:0 异构体、C18:1、C18:1 异构体、中链脂肪酸、不饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸的确定系数范围分别为 0.66 至 0.85 和 0.65 至 0.84。与 35 种脂肪酸最相关的波长在 1003 至 1145 cm-1 之间。总体而言,使用 CARS 和奶牛信息可提高基于中国荷斯坦奶牛近红外光谱的 FA 预测结果。随着数据集的扩大,还应该进行更多的验证研究。
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引用次数: 0
Functional roles of folic acid in alleviating dexamethasone-induced fatty liver syndrome in laying hens 叶酸在缓解地塞米松诱导的蛋鸡脂肪肝综合征中的功能作用
Pub Date : 2024-07-02 DOI: 10.1002/aro2.73
Xi Sun, Junjie Ma, Chaohui Wang, Zhouzheng Ren, Xin Yang, Xiaojun Yang, Yanli Liu

Fatty liver syndrome (FLS) poses a threat to the poultry industry due to its high occurrence and mortality rate. Folic acid (FA) is a coenzyme crucial for one-carbon metabolism. However, the mechanism by which FA mitigates FLS in laying hens remains elusive. In this study, 60 21-week-old Hy-Line Brown layers were divided into three groups: the Control (Con) group, the dexamethasone (DXM) group, and the DXM + FA group. Results showed that liver index was significantly increased in the DXM group. H&E and oil red O staining showed the accumulation of lipid droplets in the liver was intensified, confirming the successful establishment of an early fatty liver model without inflammation. FA significantly reversed hepatic lipid deposition, and 57 differentially expressed genes affected by FA were identified in the transcriptome analysis. Their transcriptional and translational levels indicate that in the early FLS, insulin-like growth factor 2/phosphatidylinositol-3-kinase/protein kinase B pathway related to lipid metabolism was activated; folate cycling was inhibited, while endoplasmic reticulum (ER) stress and apoptosis-related protein abundance were elevated. Dietary FA enhanced the folate circulation, reduced lipogenesis and ER stress, and apoptosis-related protein expression, thereby mitigating the lipid metabolism disturbance in FLS. Metabolomics identified 151 differential metabolites involved in early FLS occurrence, 34 of which were reversed by FA. Metabolites were also enriched in pathways related to lipid metabolism and hepatic damage. Collectively, these findings can be concluded that FA can alleviate early FLS by affecting lipogenesis, ER stress and apoptosis, which may be mediated by enhanced folate metabolism.

脂肪肝综合症(FLS)发病率和死亡率都很高,对家禽业构成威胁。叶酸(FA)是一种对一碳代谢至关重要的辅酶。然而,叶酸减轻蛋鸡FLS的机制仍未确定。本研究将 60 只 21 周龄褐羽蛋鸡分为三组:对照(Con)组、地塞米松(DXM)组和 DXM + FA 组。结果显示,DXM 组的肝脏指数明显升高。H&E和油红O染色显示,肝脏中脂滴堆积加剧,证实成功建立了无炎症的早期脂肪肝模型。在转录组分析中,发现了57个受FA影响的差异表达基因。它们的转录和翻译水平表明,在早期脂肪肝中,与脂质代谢相关的胰岛素样生长因子2/磷脂酰肌醇-3-激酶/蛋白激酶B通路被激活;叶酸循环被抑制,而内质网(ER)应激和凋亡相关蛋白丰度升高。膳食叶酸增强了叶酸循环,减少了脂肪生成和ER应激以及细胞凋亡相关蛋白的表达,从而缓解了FLS的脂质代谢紊乱。代谢组学发现了 151 种与 FLS 早期发生有关的不同代谢物,其中 34 种被脂肪酸逆转。代谢物还富集在与脂质代谢和肝损伤相关的通路中。总之,这些研究结果可以得出结论:叶酸可以通过影响脂肪生成、ER应激和细胞凋亡来缓解早期FLS,而这可能是通过增强叶酸代谢来实现的。
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引用次数: 0
The gene IFIT1 is associated with dietary copper-induced yellow fat disease in sheep 基因 IFIT1 与膳食铜诱导的绵羊黄脂病有关
Pub Date : 2024-05-30 DOI: 10.1002/aro2.66
Depeng Li, Juncai Fu

In the process of rapid fattening and rearing of meat sheep, yellow fat disease of sheep occurs frequently. This study aims to investigate the preliminary pathogenesis of yellow fat disease in sheep. Eighteen healthy sheep (4–5 months old, 34 ± 1 kg) were selected and randomly divided into three groups: the 10 ppm copper group, the 50 ppm copper group, and the 100 ppm copper group. At the end of the experiment, blood, liver, kidney, and adipose tissue samples were taken from all sheep, and measurements of each index were taken. 50 and 100 ppm copper supplementation in the diets did not significantly affect average daily gain, total cholesterol (TC), triglyceride (TG) and sorbitol dehydrogenase in sheep but significantly increased the effects on gamma-glutamyltransferase, aspartate aminotransferase, and alanine aminotransferase enzyme activities in the liver and increased the accumulation of copper in the liver. 50 and 100 ppm copper supplementation to the feed caused different levels of pathological damage to the liver, the kidney, and fat and significantly affected the brightness, redness, and yellowness of the carcass fat. Sheep in the 50 ppm copper group did not show significant clinical symptoms of yellow fat disease in the later period of the experiment, but those in the 100 ppm copper group showed significant clinical symptoms of yellow fat disease. Transcriptome analysis of sheep livers showed differential genes associated with yellow fat disease, and GO and KEGG analyses associated with yellow fat disease were performed, and further correlation analysis found that the occurrence of copper-induced yellow fat disease may be closely related to gene IFIT1.

在肉羊快速育肥和饲养过程中,绵羊黄脂病频繁发生。本研究旨在探讨绵羊黄脂病的初步发病机制。选取 18 只健康绵羊(4-5 月龄,34 ± 1 千克),随机分为三组:10 ppm 铜组、50 ppm 铜组和 100 ppm 铜组。实验结束后,对所有绵羊采集血液、肝脏、肾脏和脂肪组织样本,并测量各项指标。日粮中添加 50 和 100ppm 铜对绵羊的平均日增重、总胆固醇(TC)、甘油三酯(TG)和山梨醇脱氢酶没有显著影响,但对肝脏中的γ-谷氨酰转移酶、天冬氨酸氨基转移酶和丙氨酸氨基转移酶酶活性的影响显著增加,并增加了铜在肝脏中的积累。饲料中添加 50 和 100ppm 铜会对肝、肾和脂肪造成不同程度的病理损伤,并显著影响胴体脂肪的亮度、红度和黄度。50 ppm 铜组的绵羊在实验后期没有出现明显的黄脂病临床症状,但 100 ppm 铜组的绵羊出现了明显的黄脂病临床症状。绵羊肝脏转录组分析显示了与黄脂病相关的不同基因,并进行了与黄脂病相关的GO和KEGG分析,进一步的相关分析发现铜诱导的黄脂病的发生可能与基因IFIT1密切相关。
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引用次数: 0
Metabolomic analysis reveals the molecular mechanism related to leg abnormality in broilers 代谢组分析揭示肉鸡腿部异常的分子机制
Pub Date : 2024-05-17 DOI: 10.1002/aro2.63
Jumei Zheng, Qi Zhang, Xinxin Tang, Fan Ying, Dawei Liu, Sen Li, Ranran Liu, Jie Wen, QingHe Li, Guiping Zhao
Rapid body weight gain in broilers overloads the metabolic system of the organism, resulting in leg abnormalities, which seriously affects animal welfare and industry economics. In this study, broilers with normal and deformed leg bones were examined. Serum biochemical indices showed that the serum calcium to phosphorus ratio was extremely decreased in leg deformed group. In addition, abnormal serum lipid levels suggested a disruption in lipid metabolism. Based on widely targeted metabonomic analysis of serum and cartilage tissues, a total of nine differential metabolites (DMs) significantly associated with leg abnormalities and serum calcium and phosphorus levels were screened, including carnitine C16:0, carnitine C18:1, 3‐hydroxymethyl‐L‐tyrosine, cis‐4‐hydroxy‐D‐proline, cis‐L‐3‐hydroxyproline, trans‐4‐hydroxy‐L‐proline, and so on. Pathway analysis revealed that fatty acid degradation and arachidonic acid metabolism were enriched. Analysis of DMs in these two pathways showed that prostaglandin D2, prostaglandin J2, prostaglandin A2, 15‐keto prostaglandin F2α, and Δ12‐prostaglandin J2 significantly differed between the normal and abnormal groups. It was hypothesized that these important metabolic pathways and metabolites were involved in the metabolic regulation of leg abnormalities.
肉鸡快速增重会使机体的新陈代谢系统超负荷运转,从而导致腿部畸形,严重影响动物福利和行业经济效益。本研究对腿骨正常和畸形的肉鸡进行了研究。血清生化指标显示,腿骨畸形组的血清钙磷比极度降低。此外,血清脂质水平异常表明脂质代谢紊乱。通过对血清和软骨组织进行广泛的定向代谢分析,筛选出与腿部畸形和血清钙磷水平显著相关的9种差异代谢物(DMs),包括肉碱C16:0、肉碱C18:1、3-羟甲基-L-酪氨酸、顺式-4-羟基-D-脯氨酸、顺式-L-3-羟基脯氨酸、反式-4-羟基-L-脯氨酸等。途径分析表明,脂肪酸降解和花生四烯酸代谢富集。对这两条途径中的DMs分析表明,前列腺素D2、前列腺素J2、前列腺素A2、15-酮前列腺素F2α和Δ12-前列腺素J2在正常组和异常组之间存在显著差异。假设这些重要的代谢途径和代谢物参与了腿部异常的代谢调节。
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Animal Research and One Health
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