Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.23
Yao Li, Shen Jian, Z. Qi, W. Bing, Liu Qibin, Dai Xiyong
To investigate the early diagnosis and treatment of secondary tuberculosis complicated with COVID-19. The clinical manifestation, auxiliary examination, diagnosis and treatment of one case of secondary tuberculosis complicated with COVID-19 admitted to Wuhan Pulmonary Hospital were analyzed retrospectively. The patient had a clear history of COVID-19 exposure. The clinical manifestations were cough, expectoration, fever and gasp. Chest CT showed multiple patch, nodule, spot, strip and cavity shadow in two lungs. COVID-19 was diagnosed as early as possible by detection of SARS-CoV-2 nucleic acid in respiratory specimens. After reasonable anti-tuberculosis combined with antiviral treatment, the detection of SARS-CoV-2 nucleic acid in respiratory specimens and acid-fast bacilli smear of sputum specimens turned negative, the symptoms were relieved and discharged. In the epidemic period of COVID-19, we should pay attention to the identification of secondary pulmonary tuberculosis with COVID-19, the formulation of drug programs for the treatment of two diseases, and avoid the use of rifampicin, a liver drug enzyme inducer, so as to avoid missed treatment due to the weakened efficacy of lopinavir / ritonavir and abidol, etc. 摘要： 探讨继发性肺结核合并新型冠状病毒肺炎(coronavirus disease 2019, COVID-19)的早期诊断和治疗。回顾 性分析武汉市肺科医院收治的1例继发性肺结核合并COVID-19患者的临床表现、辅助检查及诊治经过。患者有明确 的COVID-19的接触史,临床表现为晐嗽、晐痰,发热及气喘,胸部CT可见双肺散在多发的斑片、结节、斑点、条索及空 洞影。通过呼吸道标本新型冠状病毒核酸检测尽早确诊了 COVID-19,通过合理的抗结核联合抗病毒治疗后,呼吸道 标本新型冠状病毒核酸及痰标本结核细菌涂片转阴,症状缓解出院。在COVID-19流行时期,需注意鉴别继发性肺结 核合并COVID-19情况,以及治疗两种疾病药物方案的制定,避免使用肝药酶诱导剂利福平,其会使克力芝(洛匹那韦/利托那韦）、阿比多尔等药效减弱贻误治疗。
{"title":"A case report of secondary pulmonary tuberculosis complicated with COVID-19","authors":"Yao Li, Shen Jian, Z. Qi, W. Bing, Liu Qibin, Dai Xiyong","doi":"10.13604/J.CNKI.46-1064/R.2020.12.23","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.23","url":null,"abstract":"To investigate the early diagnosis and treatment of secondary tuberculosis complicated with COVID-19. The clinical manifestation, auxiliary examination, diagnosis and treatment of one case of secondary tuberculosis complicated with COVID-19 admitted to Wuhan Pulmonary Hospital were analyzed retrospectively. The patient had a clear history of COVID-19 exposure. The clinical manifestations were cough, expectoration, fever and gasp. Chest CT showed multiple patch, nodule, spot, strip and cavity shadow in two lungs. COVID-19 was diagnosed as early as possible by detection of SARS-CoV-2 nucleic acid in respiratory specimens. After reasonable anti-tuberculosis combined with antiviral treatment, the detection of SARS-CoV-2 nucleic acid in respiratory specimens and acid-fast bacilli smear of sputum specimens turned negative, the symptoms were relieved and discharged. In the epidemic period of COVID-19, we should pay attention to the identification of secondary pulmonary tuberculosis with COVID-19, the formulation of drug programs for the treatment of two diseases, and avoid the use of rifampicin, a liver drug enzyme inducer, so as to avoid missed treatment due to the weakened efficacy of lopinavir / ritonavir and abidol, etc. 摘要： 探讨继发性肺结核合并新型冠状病毒肺炎(coronavirus disease 2019, COVID-19)的早期诊断和治疗。回顾 性分析武汉市肺科医院收治的1例继发性肺结核合并COVID-19患者的临床表现、辅助检查及诊治经过。患者有明确 的COVID-19的接触史,临床表现为晐嗽、晐痰,发热及气喘,胸部CT可见双肺散在多发的斑片、结节、斑点、条索及空 洞影。通过呼吸道标本新型冠状病毒核酸检测尽早确诊了 COVID-19,通过合理的抗结核联合抗病毒治疗后,呼吸道 标本新型冠状病毒核酸及痰标本结核细菌涂片转阴,症状缓解出院。在COVID-19流行时期,需注意鉴别继发性肺结 核合并COVID-19情况,以及治疗两种疾病药物方案的制定,避免使用肝药酶诱导剂利福平,其会使克力芝(洛匹那韦/利托那韦）、阿比多尔等药效减弱贻误治疗。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77858392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.17
Wang Yu, Yang Pei-cai, Y. Qi, Xie Chao-yong
Objective To evaluate the control effect of effective control for intestinal nematode disease, and to analyze the dynamic change of epidemic trend, we provide basis for adjusting control strategy of intestinal nematode disease. Methods The data of control strategies in Nanjing from 2001 to 2019 were analyzed, including monitoring data of intestinal nematode disease, drug deworming, health education and rural toilet improvement. Results From 2001 to 2019, 191 172 person-times of residents were surveyed fecal examination in Nanjing City, and 2 596 person-times of residents were detected with intestinal nematode infections, with annual average infection rate 1.36%. The positive rate of human intestinal nematode infections showed a significantly declining trend in total ( P <0.01). In Nanjing, intestinal nematode infections were mainly caused by ascaris (0.90%, 1 724 cases), hookworm (0.30%, 564 cases), pinworm (0.09%, 179 cases) and trichuris (0.07%, 129 cases). Among them, 2 345 cases (96.98%) had mild infection. In 2001, the infection rate was the highest (7.00%), while in 2018 and 2019, the infection rate was the lowest (both 0.01%). The difference of infection rate in each year was statistically significant ( P <0.01). 114 940 person-times of children under 12 years old were surveyed for the special surveillance of pinworm in Nanjing, and 500 cases were detected with positive infection. Moreover, the positive rate showed a significantly decreasing trend in total ( P <0.01). At the end of 2019, 9 237.5 million person-times have been treated with medicine, the awareness rate of intestinal nematode control among the population in Nanjing reached more than 90%, the penetration rate of harmless toilets was 99.99%. Conclusion The effective control of intestinal nematode disease in Nanjing has achieved remarkable results, however, the surveillance network of intestinal nematode disease still should be improved, and health education in key population groups should be strengthened, in order to adjust control strategies in time, and effectively consolidate the effectiveness of the control of intestinal nematode disease. 摘要：目的 评价有效控制肠道线虫病项目的防治效果,分析流行趋势的动态变化,为调整肠道线虫病防控策略 提供依据。 方法 对2001—2019年南京市肠道线虫病监测数据、以及实施驱虫服药、健康教育、农村改厕等防治策略 资料进行统计分析。 结果 2001—2019年,南京市共粪检191 172人次,检出肠道线虫阳性感染2 596人次,年平均感 染率为1.36%,人群肠道线虫感染率逐年下降趋势( P <0.01)。南京市肠道线虫感染以蛔虫、钩虫、蛲虫、鞭虫为主,且以 轻度感染为主,共计2 345 例（占96.98%),感染人数分别为1 724例、564例、179例和129例,感染率分别为0.90%、0.30%,0.09%和0.07%。以2001年的人群感染率最髙,为7.00%,2018年和2019年的感染率最低,均为0.01%,各年份人 群感染率差异有统计学意义( P <0.01)。南京市儿童蛲虫病专项监测累计检査儿童114 940人次,阳性感染者500人次。儿童蛲虫感染率亦呈现逐年下降趋势( P <0.01)。截至2019年12月,南京市已累计服药驱虫923 750万人次,人群肠道 线虫病卫生知识知晓率大于90%,无害化卫生户厕普及率为99.99%。 结论 南京市有效控制肠道线虫病防治项目取 得显著效果,但仍需不断完善肠道线虫病的监测网络,加强重点人群健康教育,适时调整防控策略,有效巩固肠道线虫 病的防治成效。
{"title":"Evaluation of control effect of implement effective control for intestinal nematode disease in Nanjing","authors":"Wang Yu, Yang Pei-cai, Y. Qi, Xie Chao-yong","doi":"10.13604/J.CNKI.46-1064/R.2020.12.17","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.17","url":null,"abstract":"Objective To evaluate the control effect of effective control for intestinal nematode disease, and to analyze the dynamic change of epidemic trend, we provide basis for adjusting control strategy of intestinal nematode disease. Methods The data of control strategies in Nanjing from 2001 to 2019 were analyzed, including monitoring data of intestinal nematode disease, drug deworming, health education and rural toilet improvement. Results From 2001 to 2019, 191 172 person-times of residents were surveyed fecal examination in Nanjing City, and 2 596 person-times of residents were detected with intestinal nematode infections, with annual average infection rate 1.36%. The positive rate of human intestinal nematode infections showed a significantly declining trend in total ( P <0.01). In Nanjing, intestinal nematode infections were mainly caused by ascaris (0.90%, 1 724 cases), hookworm (0.30%, 564 cases), pinworm (0.09%, 179 cases) and trichuris (0.07%, 129 cases). Among them, 2 345 cases (96.98%) had mild infection. In 2001, the infection rate was the highest (7.00%), while in 2018 and 2019, the infection rate was the lowest (both 0.01%). The difference of infection rate in each year was statistically significant ( P <0.01). 114 940 person-times of children under 12 years old were surveyed for the special surveillance of pinworm in Nanjing, and 500 cases were detected with positive infection. Moreover, the positive rate showed a significantly decreasing trend in total ( P <0.01). At the end of 2019, 9 237.5 million person-times have been treated with medicine, the awareness rate of intestinal nematode control among the population in Nanjing reached more than 90%, the penetration rate of harmless toilets was 99.99%. Conclusion The effective control of intestinal nematode disease in Nanjing has achieved remarkable results, however, the surveillance network of intestinal nematode disease still should be improved, and health education in key population groups should be strengthened, in order to adjust control strategies in time, and effectively consolidate the effectiveness of the control of intestinal nematode disease. 摘要：目的 评价有效控制肠道线虫病项目的防治效果,分析流行趋势的动态变化,为调整肠道线虫病防控策略 提供依据。 方法 对2001—2019年南京市肠道线虫病监测数据、以及实施驱虫服药、健康教育、农村改厕等防治策略 资料进行统计分析。 结果 2001—2019年,南京市共粪检191 172人次,检出肠道线虫阳性感染2 596人次,年平均感 染率为1.36%,人群肠道线虫感染率逐年下降趋势( P <0.01)。南京市肠道线虫感染以蛔虫、钩虫、蛲虫、鞭虫为主,且以 轻度感染为主,共计2 345 例（占96.98%),感染人数分别为1 724例、564例、179例和129例,感染率分别为0.90%、0.30%,0.09%和0.07%。以2001年的人群感染率最髙,为7.00%,2018年和2019年的感染率最低,均为0.01%,各年份人 群感染率差异有统计学意义( P <0.01)。南京市儿童蛲虫病专项监测累计检査儿童114 940人次,阳性感染者500人次。儿童蛲虫感染率亦呈现逐年下降趋势( P <0.01)。截至2019年12月,南京市已累计服药驱虫923 750万人次,人群肠道 线虫病卫生知识知晓率大于90%,无害化卫生户厕普及率为99.99%。 结论 南京市有效控制肠道线虫病防治项目取 得显著效果,但仍需不断完善肠道线虫病的监测网络,加强重点人群健康教育,适时调整防控策略,有效巩固肠道线虫 病的防治成效。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77448800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.04
Y. Jie, Liao Zhengfa, W. Xiaoqiang, L. Qiao
Objective Through the relationship between climatic factors such as average temperature, relative humidity, sunshine duration and the number of outpatient visits of psoriasis patients, the influence of climatic factors on the pathogenesis of psoriasis was discussed. Methods We collected daily local outpatient visits' data for psoriasis from The Fifth People's Hospital of Hainan Province in Haikou, Hainan, from January 1 2016 to December 31 2018. Climatic factors, including average temperature, relative humidity, sunshine duration, during the same time were collected from Hainan Meteorological Bureau. Firstly, sequence diagrams were used to observe the average temperature, relative humidity and sunshine duration to determine the stationarity of sequence diagrams. Secondly, through the autocorrelation figure, partial autocorrelation, periodic correlation diagram was used to determine whether there is the correlation, and delay order number. The simple linear regression was carried out to determine the relationship between the various factors. Finally, the summation of time series analysis of the independent variable ARIMAX (Autoregressive Integrated Moving Average with an Independent variable) was used to fit the model and ultimately determine which climatic factors contribute to psoriasis. ARIMAX model parameters are calculated by auto-arima function in R language software. Results Weekly outpatient visits for psoriasis was correlated with the weekly average sunshine duration and the weekly average temperature, but not with humidity. There was a significant correlation between the weekly average temperature and the sunshine duration. The regression equation between them was as follows: Average temperature =183.427 + 0.159 23x duration of sunshine duration ( R 2 =0.512 4, P <0.001). Conclusions Changes in average temperature or sunshine duration can cause changes in the number of outpatient visits for psoriasis patients in Hainan province. These two climatic factors may be the influencing factors for the incidence of psoriasis patients in Hainan Province. 摘要：目的 通过平均气温、相对湿度和日照时长等气候因素与银屑病患者门诊就诊人数之间的联系, 探讨气候 因素对银屑病发病的影响。 方法 收集自2016年1月1日到2018年12月31日海南省第五人民医院银屑病患者门诊 每周的就诊人数。同时收集同一时间的气象资料 , 包括平均气温、相对湿度 、 日照时长等, 所有气候数据均来自海南省 气象局。首先通过时序图观察获知平均气温、相对湿度、日照时长的变化趋势, 初步确定时序图的平稳性, 再通过自相 关图、偏自相关相关图确定是否存在自相关性、周期性、以及延迟阶数, 并通过简单线性回归确定各因素之间的关系, 最后通过时间序列分析中带自变量的求和自回归移动平均模型（Autoregressive Integrated Moving Average with an Independent variable, ARIMAX)来拟合模型, 最终确定哪些气候因素是银屑病发病的影响因素。ARIMAX模型参数通过R 语言软件中的自动定阶auto.arima函数来计算获得。 结果 每周银屑病就诊人数与周平均日照时长和周平均气温之间 存在相关性, 与相对湿度不存相关性;而周平均气温与周日照时长之间存在明显相关性, 两者间回归方程:平均气温= 183.427+0.159 23X周日照时长( R 2 =0.512 4, P <0.001)。 结论 平均气温或日照时长的变化可以引起海南省银屑病患者 门诊就诊人数的变化, 此两项气候因素可能是海南省银屑病患者发病的影响因素。
目的通过平均气温、相对湿度、日照时数等气候因素与银屑病患者门诊次数的关系，探讨气候因素对银屑病发病的影响。方法收集海南省海口市海南省第五人民医院2016年1月1日至2018年12月31日当地每日银屑病门诊人次数据。同期气候因子包括平均气温、相对湿度、日照时数等数据均由海南省气象局采集。首先，利用序列图观测平均温度、相对湿度和日照时数，确定序列图的平稳性;其次，通过自相关图、偏自相关图、周期相关图确定是否存在相关性，以及时延阶数。通过简单的线性回归来确定各因素之间的关系。最后，利用自变量ARIMAX (Autoregressive Integrated Moving Average with a自变量)时间序列分析的总和对模型进行拟合，最终确定哪些气候因素对牛皮癣有影响。利用R语言软件中的auto-arima函数计算ARIMAX模型参数。结果银屑病患者每周门诊次数与周平均日照时数和周平均气温相关，与湿度无关。周平均气温与日照时数呈显著相关。回归方程为平均气温=183.427 + 0.159 23 ×日照时数(r2 =0.512 4, P <0.001)。结论平均气温和日照时数的变化可引起海南省银屑病患者门诊次数的变化。这两个气候因素可能是海南省银屑病发病的影响因素。摘要：目的 通过平均气温、相对湿度和日照时长等气候因素与银屑病患者门诊就诊人数之间的联系, 探讨气候 因素对银屑病发病的影响。 方法 收集自2016年1月1日到2018年12月31日海南省第五人民医院银屑病患者门诊 每周的就诊人数。同时收集同一时间的气象资料 , 包括平均气温、相对湿度 、 日照时长等, 所有气候数据均来自海南省 气象局。首先通过时序图观察获知平均气,温相对湿度,日照时长的变化趋势,初步确定时序图的平稳性,再通过自相关图,偏自相关相关图确定是否存在自相关性,周期性,以及延迟阶数,并通过简单线性回归确定各因素之间的关系,最后通过时间序列分析中带自变量的求和自回归移动平均模型(自回归综合移动平均线与一个独立的变量,ARIMAX)来拟合模型,最终确定哪些气候因素是银屑病发病的影响因素。arima。结果每周银屑病就诊人数与周平均日照时长和周平均气温之间存在相关性,与相对湿度不存相关性,而周平均气温与周日照时长之间存在明显相关性,两者间回归方程:平均气温= 183.427 + 0.159 x周23日照时长(4 R 2 = 0.512, P < 0.001)。结论 平均气温或日照时长的变化可以引起海南省银屑病患者 门诊就诊人数的变化, 此两项气候因素可能是海南省银屑病患者发病的影响因素。
{"title":"A time-series analysis on the effects of climate on outpatient visits for psoriasis","authors":"Y. Jie, Liao Zhengfa, W. Xiaoqiang, L. Qiao","doi":"10.13604/J.CNKI.46-1064/R.2020.12.04","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.04","url":null,"abstract":"Objective Through the relationship between climatic factors such as average temperature, relative humidity, sunshine duration and the number of outpatient visits of psoriasis patients, the influence of climatic factors on the pathogenesis of psoriasis was discussed. Methods We collected daily local outpatient visits' data for psoriasis from The Fifth People's Hospital of Hainan Province in Haikou, Hainan, from January 1 2016 to December 31 2018. Climatic factors, including average temperature, relative humidity, sunshine duration, during the same time were collected from Hainan Meteorological Bureau. Firstly, sequence diagrams were used to observe the average temperature, relative humidity and sunshine duration to determine the stationarity of sequence diagrams. Secondly, through the autocorrelation figure, partial autocorrelation, periodic correlation diagram was used to determine whether there is the correlation, and delay order number. The simple linear regression was carried out to determine the relationship between the various factors. Finally, the summation of time series analysis of the independent variable ARIMAX (Autoregressive Integrated Moving Average with an Independent variable) was used to fit the model and ultimately determine which climatic factors contribute to psoriasis. ARIMAX model parameters are calculated by auto-arima function in R language software. Results Weekly outpatient visits for psoriasis was correlated with the weekly average sunshine duration and the weekly average temperature, but not with humidity. There was a significant correlation between the weekly average temperature and the sunshine duration. The regression equation between them was as follows: Average temperature =183.427 + 0.159 23x duration of sunshine duration ( R 2 =0.512 4, P <0.001). Conclusions Changes in average temperature or sunshine duration can cause changes in the number of outpatient visits for psoriasis patients in Hainan province. These two climatic factors may be the influencing factors for the incidence of psoriasis patients in Hainan Province. 摘要：目的 通过平均气温、相对湿度和日照时长等气候因素与银屑病患者门诊就诊人数之间的联系, 探讨气候 因素对银屑病发病的影响。 方法 收集自2016年1月1日到2018年12月31日海南省第五人民医院银屑病患者门诊 每周的就诊人数。同时收集同一时间的气象资料 , 包括平均气温、相对湿度 、 日照时长等, 所有气候数据均来自海南省 气象局。首先通过时序图观察获知平均气温、相对湿度、日照时长的变化趋势, 初步确定时序图的平稳性, 再通过自相 关图、偏自相关相关图确定是否存在自相关性、周期性、以及延迟阶数, 并通过简单线性回归确定各因素之间的关系, 最后通过时间序列分析中带自变量的求和自回归移动平均模型（Autoregressive Integrated Moving Average with an Independent variable, ARIMAX)来拟合模型, 最终确定哪些气候因素是银屑病发病的影响因素。ARIMAX模型参数通过R 语言软件中的自动定阶auto.arima函数来计算获得。 结果 每周银屑病就诊人数与周平均日照时长和周平均气温之间 存在相关性, 与相对湿度不存相关性;而周平均气温与周日照时长之间存在明显相关性, 两者间回归方程:平均气温= 183.427+0.159 23X周日照时长( R 2 =0.512 4, P <0.001)。 结论 平均气温或日照时长的变化可以引起海南省银屑病患者 门诊就诊人数的变化, 此两项气候因素可能是海南省银屑病患者发病的影响因素。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"105 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79739051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.11
Gan Yongxia, L. Hao, Yang Zheng-rong, Zhao Jin, Shi Xiangdong, Gao Shilan
Objective To compare the results of HIV rapid detection reagent in saliva and blood in MSM in Shenzhen, and we clarify the role of saliva rapid detection in HIV screening in this population. Methods The exudate in saliva and blood samples of the same subjects in 393 MSM in Shenzhen were collected, and the HIV immunoblotting kit was used as the reference reagent to confirm the consistency of salivary antibody detection kit (colloidal gold method) and human immunodeficiency virus (HIV) type 1+2 antibody detection kit (colloidal selenium method). Results Totally 393 MSM were recruited. The true positive, false positive, true negative, false negative cases for saliva test reagents were 120, 0, 272, 1, respectively. Those for blood test reagents were 121, 3, 269, 0, respectively. The detection results of the HIV western blot kit were taken as the gold standard to compare and analyze the detection results of saliva and blood detection reagent. The sensitivity, specificity, false-positive rate, false-negative rate and accuracy of exudate in saliva test reagents were 99.2%, 100.0%, 0.0%, 0.8% and 99.8%, respectively. Those of blood test reagents were 100.0%, 98.9%, 1.10%, 0.0% and 99.2%, respectively. Kappa test analysis showed that the results of rapid saliva and blood test were consistent with HIV diagnosis, and the Kappa values were 0.994 and 0.982, respectively. McNemar paired chi-square test was used to analyze the results of saliva and blood rapid test, and the difference was not statistically significant ( P =0.125). Conclusion Saliva and blood screening results have a good consistency, exudate in saliva detection is noninvasive and fast, this method is worth promoting for HIV self-screening in high risk population. 摘要：目的 分析深圳市男男性行为者(MSM)中唾液和血液HIV快速检测试剂结果, 探讨唾液快速检测法在MSM 人群HIV筛査的作用。 方法 同时采集深圳市393名MSM人群的唾液和血液样本 , 使用HIV免疫印迹试剂盒作为参 考试剂, 比较唾液抗体检测试剂盒(胶体金法)和人类免疫缺陷病毒(HIV) 1+2型抗体检测试剂盒(胶体砸法)两种快速 检测试剂结果的符合性。 结果 在393份MSM有效样本中, 唾液检测试剂的真阳性为120例, 假阳性为0例, 真阴性为 272例, 假阴性为1例。血液快速法真阳性为121例, 假阳性为3例, 真阴性为269例, 假阴性为0例。以HIV免疫印迹试 剂盒检测结果为金标准 , 唾液检测试剂的灵敏度为99.2%, 特异度为100.0%, 假阳性率为0.0%, 假阴性率为0.8%, 准确 度为99.8%。血液检测试剂的灵敏度为100.0%,特异度为98.9%, 假阳性率为1.1%, 假阴性率为0.0%, 准确度为 99.2%。Kappa检验分析显示唾液、血液快速检测法与HIV诊断结果存在一致性, Kappa值分别为0.994和0.982; McNemar配对卡方检验分析唾液和血液快速法的检测结果发现二者的差异无统计学意义( P =0.125)。 结论 唾液与血液的 筛査结果具有较好一致性 , 基于唾液检测安全无创、快速, 该方法值得在髙危人群HIV抗体自我筛査中推广使用
{"title":"Comparative study on HIV rapid detection of saliva and blood in MSM of Shenzhen","authors":"Gan Yongxia, L. Hao, Yang Zheng-rong, Zhao Jin, Shi Xiangdong, Gao Shilan","doi":"10.13604/J.CNKI.46-1064/R.2020.12.11","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.11","url":null,"abstract":"Objective To compare the results of HIV rapid detection reagent in saliva and blood in MSM in Shenzhen, and we clarify the role of saliva rapid detection in HIV screening in this population. Methods The exudate in saliva and blood samples of the same subjects in 393 MSM in Shenzhen were collected, and the HIV immunoblotting kit was used as the reference reagent to confirm the consistency of salivary antibody detection kit (colloidal gold method) and human immunodeficiency virus (HIV) type 1+2 antibody detection kit (colloidal selenium method). Results Totally 393 MSM were recruited. The true positive, false positive, true negative, false negative cases for saliva test reagents were 120, 0, 272, 1, respectively. Those for blood test reagents were 121, 3, 269, 0, respectively. The detection results of the HIV western blot kit were taken as the gold standard to compare and analyze the detection results of saliva and blood detection reagent. The sensitivity, specificity, false-positive rate, false-negative rate and accuracy of exudate in saliva test reagents were 99.2%, 100.0%, 0.0%, 0.8% and 99.8%, respectively. Those of blood test reagents were 100.0%, 98.9%, 1.10%, 0.0% and 99.2%, respectively. Kappa test analysis showed that the results of rapid saliva and blood test were consistent with HIV diagnosis, and the Kappa values were 0.994 and 0.982, respectively. McNemar paired chi-square test was used to analyze the results of saliva and blood rapid test, and the difference was not statistically significant ( P =0.125). Conclusion Saliva and blood screening results have a good consistency, exudate in saliva detection is noninvasive and fast, this method is worth promoting for HIV self-screening in high risk population. 摘要：目的 分析深圳市男男性行为者(MSM)中唾液和血液HIV快速检测试剂结果, 探讨唾液快速检测法在MSM 人群HIV筛査的作用。 方法 同时采集深圳市393名MSM人群的唾液和血液样本 , 使用HIV免疫印迹试剂盒作为参 考试剂, 比较唾液抗体检测试剂盒(胶体金法)和人类免疫缺陷病毒(HIV) 1+2型抗体检测试剂盒(胶体砸法)两种快速 检测试剂结果的符合性。 结果 在393份MSM有效样本中, 唾液检测试剂的真阳性为120例, 假阳性为0例, 真阴性为 272例, 假阴性为1例。血液快速法真阳性为121例, 假阳性为3例, 真阴性为269例, 假阴性为0例。以HIV免疫印迹试 剂盒检测结果为金标准 , 唾液检测试剂的灵敏度为99.2%, 特异度为100.0%, 假阳性率为0.0%, 假阴性率为0.8%, 准确 度为99.8%。血液检测试剂的灵敏度为100.0%,特异度为98.9%, 假阳性率为1.1%, 假阴性率为0.0%, 准确度为 99.2%。Kappa检验分析显示唾液、血液快速检测法与HIV诊断结果存在一致性, Kappa值分别为0.994和0.982; McNemar配对卡方检验分析唾液和血液快速法的检测结果发现二者的差异无统计学意义( P =0.125)。 结论 唾液与血液的 筛査结果具有较好一致性 , 基于唾液检测安全无创、快速, 该方法值得在髙危人群HIV抗体自我筛査中推广使用","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73633223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.03
Q. Yi, Zeng Jun, L. Bao, Guo Guimin, Huang Jun, Wang Ming-liu
Objective To describe and compare the profiles and evolution of antimicrobial resistance in Salmonella paratyphi A isolates collected from different years in Fuchuan County of Guangxi, through in vitro drug sensitivity tests, and we provide scientific evidence for formulating surveillance programs and control policies. Methods A panel of 122 isolates of Salmonella paratyphi A were obtained from 3 outbreaks in Fuchuan County of Guangxi, 2000-2018, and tested for minimum inhibitory concentrations (MIC) of antibiotics by the broth dilution method. Results The 122 strains were all susceptible (100.00%) to ampicillin, ampicilli / sulbactam, cotrimoxazole, cefotaxime, ceftazidime, gentamicin, azithromycin, imipenem and sulfamethoxazole. Over 90.00% of them were susceptible to tetracycline, chloramphenicol and cefoxitin. Only 71.31% of them were susceptible to cefazolin. They manifests as drug-resistance and intermediate to quinolones: The intermediate rate of ciprofloxacin is 97.54%; nalidixic acid is entirely 100.00% resistance. Cefotaxim, ceftazidime and gentamicin show the best antibacterial effects, and the phenomenon of multidrug resistance is not severity. No strains resistant to third-generation cephalosporin were detected. Conclusion Third-generation cephalosporins should be accepted as a drug of first choice for Salmonella paratyphi A. And quinolones should be used with caution. Enhanced surveillance of antimicrobial resistance in Salmonella paratyphi A is essential to provide an evidence base for empirical treatment protocols. 摘要：目的 通过体外药物敏感性试验, 了解广西富川县不同年度甲型副伤寒沙门菌对15种抗生素的敏感性及耐 药变迁, 为甲型副伤寒的疾病监测及防治策略提供实验数据。 方法 采用微量肉汤稀释法对广西富川县2000—2018 年三起甲型副伤寒疫情分离的122株甲型副伤寒沙门菌进行药敏试验, 分析不同最低抑菌浓度（MIC)检测结果。 结果 122株甲型副伤寒沙门菌对氨苄西林(AMP)、氨苄西林/舒巴坦(AMS)、复方新诺明(SXT)、头孢噻肟(CTX)、头孢 他啶(CAZ)、庆大霉素（GEN)、阿奇霉素(AZI)、亚胺培南(IMI)和磺胺异恶唑(SUL)等9种药物敏感率达100.00%, 四环 素(TET)、氯霉素(CHL)和头孢西丁(CFX)敏感率也达90.00%以上, 头孢唑啉(CFZ)敏感率仅为71.31%;对喹诺酮类药 物主要表现为耐药和中介:环丙沙星(CIP)中介率为97.54%, 萘啶酸(NAL)100.00%耐药。CTX、CAZ和GEN三种药物 抑菌效果最佳, 多重耐药现象不严重, 未出现耐三代头孢药物菌株。 结论 第三代头孢药物可作为治疗甲型副伤寒 的首选药物, 慎用喹诺酮类药物；同时应加强常用抗生素耐药监测, 结合本地实际情况, 合理选用治疗甲型副伤寒的首 选和常规药物。
{"title":"Antimicrobial resistance profiles of Salmonella paratyphi A isolates collected from 3 outbreaks in Fuchuan County of Guangxi, 2000-2018","authors":"Q. Yi, Zeng Jun, L. Bao, Guo Guimin, Huang Jun, Wang Ming-liu","doi":"10.13604/J.CNKI.46-1064/R.2020.12.03","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.03","url":null,"abstract":"Objective To describe and compare the profiles and evolution of antimicrobial resistance in Salmonella paratyphi A isolates collected from different years in Fuchuan County of Guangxi, through in vitro drug sensitivity tests, and we provide scientific evidence for formulating surveillance programs and control policies. Methods A panel of 122 isolates of Salmonella paratyphi A were obtained from 3 outbreaks in Fuchuan County of Guangxi, 2000-2018, and tested for minimum inhibitory concentrations (MIC) of antibiotics by the broth dilution method. Results The 122 strains were all susceptible (100.00%) to ampicillin, ampicilli / sulbactam, cotrimoxazole, cefotaxime, ceftazidime, gentamicin, azithromycin, imipenem and sulfamethoxazole. Over 90.00% of them were susceptible to tetracycline, chloramphenicol and cefoxitin. Only 71.31% of them were susceptible to cefazolin. They manifests as drug-resistance and intermediate to quinolones: The intermediate rate of ciprofloxacin is 97.54%; nalidixic acid is entirely 100.00% resistance. Cefotaxim, ceftazidime and gentamicin show the best antibacterial effects, and the phenomenon of multidrug resistance is not severity. No strains resistant to third-generation cephalosporin were detected. Conclusion Third-generation cephalosporins should be accepted as a drug of first choice for Salmonella paratyphi A. And quinolones should be used with caution. Enhanced surveillance of antimicrobial resistance in Salmonella paratyphi A is essential to provide an evidence base for empirical treatment protocols. 摘要：目的 通过体外药物敏感性试验, 了解广西富川县不同年度甲型副伤寒沙门菌对15种抗生素的敏感性及耐 药变迁, 为甲型副伤寒的疾病监测及防治策略提供实验数据。 方法 采用微量肉汤稀释法对广西富川县2000—2018 年三起甲型副伤寒疫情分离的122株甲型副伤寒沙门菌进行药敏试验, 分析不同最低抑菌浓度（MIC)检测结果。 结果 122株甲型副伤寒沙门菌对氨苄西林(AMP)、氨苄西林/舒巴坦(AMS)、复方新诺明(SXT)、头孢噻肟(CTX)、头孢 他啶(CAZ)、庆大霉素（GEN)、阿奇霉素(AZI)、亚胺培南(IMI)和磺胺异恶唑(SUL)等9种药物敏感率达100.00%, 四环 素(TET)、氯霉素(CHL)和头孢西丁(CFX)敏感率也达90.00%以上, 头孢唑啉(CFZ)敏感率仅为71.31%;对喹诺酮类药 物主要表现为耐药和中介:环丙沙星(CIP)中介率为97.54%, 萘啶酸(NAL)100.00%耐药。CTX、CAZ和GEN三种药物 抑菌效果最佳, 多重耐药现象不严重, 未出现耐三代头孢药物菌株。 结论 第三代头孢药物可作为治疗甲型副伤寒 的首选药物, 慎用喹诺酮类药物；同时应加强常用抗生素耐药监测, 结合本地实际情况, 合理选用治疗甲型副伤寒的首 选和常规药物。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83130803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604//J.CNKI.46-1064/R.2020.12.15
Hung Jing, Li Yang, Liu Wenwen, Zhang Jiaxi
Objective To understand the anxiety status and related influencing factors of nurses fighting against coronavirus disease 2019 (COVID-19), and we provide guidance for improving first-line clinical nurses'anxiety. Methods The 196 first-line clinical nurses who fought against COVID-19 in the Sixth People's Hospital of Hebei Province from January 24 to February 25, 2020 were selected. Self rating Anxiety Scale (SAS) was used to investigate the anxiety status of clinical first-line nurses. Single factor and multiple stepwise Logistic regression were used to analyze the risk factors affecting anxiety of clinical first-line nurses. The area under curve of the ROC curve was used to judge the prediction ability of the model to the anxiety of clinical first-line nurses. Results Among 196 clinical first-line nurses, 82 had anxiety, the proportion was 41.8% (82/196). Univariate analysis showed that fatigue, public health emergencies, personality type, informing family members in the first line, only child, panic and sleep quality were related to anxiety of clinical first-line nurses ( P <0.05). Multiple stepwise Logistic regression analysis showed that fatigue [ OR (95% CI ) =1.952 (1.204-4.216)], introversion [ OR (95% CI ) =1.846 (1.115-3.938)],panic [ OR (95%CI) =2.428 (1.537-6.620)] and poor sleep quality [ OR (95%CI) =2.713 (1.715-7.258)] were independent risk factors for anxiety of clinical first-line nurses ( P <0.05), while participation in public health emergencies [ OR (95% CI ) =0.628(0.316-0.964)] and informing family members to be on the first-line [ OR (95% CI )=0.593 (0.248-0.917)] were protective factors for anxiety of clinical first-line nurses ( P <0.05). The area under curve of ROC was 0.928 (95%CI: 0.868-0.984, P <0.05). Conclusion Some of the first-line clinical nurses fighting against NCP have anxiety, and there are many influencing factors, so psychological counseling and humanistic care should be taken to intervene to improve the anxiety of nurses. 摘要：目的 了解抗击新型冠状病毒肺炎(新冠肺炎)护士的焦虑状况及其相关影响因素,为改善临床一线护士的 焦虑提供指导。 方法 选取2020年1月24日一2020年2月25日河北省第六人民医院抗击新冠肺炎的196名临床一线 护士。采用焦虑自评量表(SAS)对临床一线护士的焦虑状况进行调査,应用单因素及多元逐步Logistic回归分析影响 临床一线护士焦虑的危险因素。应用受试者工作特征( ROC )曲线下面积判断该模型对临床一线护士焦虑的预测能力。 结果 临床一线护士 196人中,82人有焦虑,构成比为41.8%(82/196)。单因素分析显示,容易疲劳、参加过突发公共卫 生事件、性格类型、告知家人在一线、独生子女、恐慌及睡眠质量与临床一线护士焦虑有关( P <0.05)。多元逐步Logistic 回归分析显示,容易疲劳[ OR (95% CI ) = 1.952(1.204~4.216)]、性格内向[ OR (95% CI )=1.846(1.115~3.938)]、恐慌[ OR (95% CI )=2.428(1.537~6.620)]及睡眠质量差[ OR (95% CI )=2.713(1.715~7.258)]是影响临床一线护士焦虑的独立危险 因素( P <0.05),而参加过突发公共卫生事件[ OR (95% CI )=0.628(0.316~0.964)]及告知家人在一线[ OR (95% CI )=0.593 (0.248~0.917)]是临床一线护士焦虑的保护因素( P <0.05)。该模型ROC曲线下面积为0.928(95% CI :0.868~0.984, P <0.05)。 结论 部分抗击新冠肺炎临床一线护士存在焦虑,其影响因素较多,应采取心理疏导和人文关怀等措施进行干 预,以改善护士的焦虑状况。
{"title":"Anxiety status and its influencing factors of nurses fighting COVID-19","authors":"Hung Jing, Li Yang, Liu Wenwen, Zhang Jiaxi","doi":"10.13604//J.CNKI.46-1064/R.2020.12.15","DOIUrl":"https://doi.org/10.13604//J.CNKI.46-1064/R.2020.12.15","url":null,"abstract":"Objective To understand the anxiety status and related influencing factors of nurses fighting against coronavirus disease 2019 (COVID-19), and we provide guidance for improving first-line clinical nurses'anxiety. Methods The 196 first-line clinical nurses who fought against COVID-19 in the Sixth People's Hospital of Hebei Province from January 24 to February 25, 2020 were selected. Self rating Anxiety Scale (SAS) was used to investigate the anxiety status of clinical first-line nurses. Single factor and multiple stepwise Logistic regression were used to analyze the risk factors affecting anxiety of clinical first-line nurses. The area under curve of the ROC curve was used to judge the prediction ability of the model to the anxiety of clinical first-line nurses. Results Among 196 clinical first-line nurses, 82 had anxiety, the proportion was 41.8% (82/196). Univariate analysis showed that fatigue, public health emergencies, personality type, informing family members in the first line, only child, panic and sleep quality were related to anxiety of clinical first-line nurses ( P <0.05). Multiple stepwise Logistic regression analysis showed that fatigue [ OR (95% CI ) =1.952 (1.204-4.216)], introversion [ OR (95% CI ) =1.846 (1.115-3.938)],panic [ OR (95%CI) =2.428 (1.537-6.620)] and poor sleep quality [ OR (95%CI) =2.713 (1.715-7.258)] were independent risk factors for anxiety of clinical first-line nurses ( P <0.05), while participation in public health emergencies [ OR (95% CI ) =0.628(0.316-0.964)] and informing family members to be on the first-line [ OR (95% CI )=0.593 (0.248-0.917)] were protective factors for anxiety of clinical first-line nurses ( P <0.05). The area under curve of ROC was 0.928 (95%CI: 0.868-0.984, P <0.05). Conclusion Some of the first-line clinical nurses fighting against NCP have anxiety, and there are many influencing factors, so psychological counseling and humanistic care should be taken to intervene to improve the anxiety of nurses. 摘要：目的 了解抗击新型冠状病毒肺炎(新冠肺炎)护士的焦虑状况及其相关影响因素,为改善临床一线护士的 焦虑提供指导。 方法 选取2020年1月24日一2020年2月25日河北省第六人民医院抗击新冠肺炎的196名临床一线 护士。采用焦虑自评量表(SAS)对临床一线护士的焦虑状况进行调査,应用单因素及多元逐步Logistic回归分析影响 临床一线护士焦虑的危险因素。应用受试者工作特征( ROC )曲线下面积判断该模型对临床一线护士焦虑的预测能力。 结果 临床一线护士 196人中,82人有焦虑,构成比为41.8%(82/196)。单因素分析显示,容易疲劳、参加过突发公共卫 生事件、性格类型、告知家人在一线、独生子女、恐慌及睡眠质量与临床一线护士焦虑有关( P <0.05)。多元逐步Logistic 回归分析显示,容易疲劳[ OR (95% CI ) = 1.952(1.204~4.216)]、性格内向[ OR (95% CI )=1.846(1.115~3.938)]、恐慌[ OR (95% CI )=2.428(1.537~6.620)]及睡眠质量差[ OR (95% CI )=2.713(1.715~7.258)]是影响临床一线护士焦虑的独立危险 因素( P <0.05),而参加过突发公共卫生事件[ OR (95% CI )=0.628(0.316~0.964)]及告知家人在一线[ OR (95% CI )=0.593 (0.248~0.917)]是临床一线护士焦虑的保护因素( P <0.05)。该模型ROC曲线下面积为0.928(95% CI :0.868~0.984, P <0.05)。 结论 部分抗击新冠肺炎临床一线护士存在焦虑,其影响因素较多,应采取心理疏导和人文关怀等措施进行干 预,以改善护士的焦虑状况。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"16 1","pages":"1188-1192"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85659617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.05
Fan Dongying, Gao Na, Jiang Chunhong, Wu Na
Objective To evaluate the antivirus activity of Xiyanping Injection against Dengue virus serotype 2 (DENV2) in vitro and in vivo. Methods A human hepatoma cell line HepG2 was treated with Xiyanping Injection through different ways, including the direct inactivation of virus, the inhibition of virus entry, administration after virus exposure and combination of pre- and post-exposure administration. The supernatant and cells were harvested in different time point. Then the virus antigen positive cells were detected by indirect immunofluorescence assay (IFA), and the viral RNA was measured by quantitative real-time PCR (qPCR). Moreover, antiviral activity of Xiyanping Injection was further detected with the HepG2 transplanted SCID mice. The mice were intraperitoneally injected with different doses (1,2,4 mg/mouse) of Xiyanping Injection for 4 days following the challenge of DENV2. The organs and sera in different groups were sampled on the sixth day post infection. Then, the numbers of virus antigen positive cells in spleen, liver and sera of mice were detected by IFA, and the survival rate of each group was monitored for 14 days. Results Xiyanping Injection can inactivate DENV2 and inhibit the entry of DENV2 into cells. The replication of DENV2 was significantly inhibited by Xiyanping Injection with the aforementioned medication in a dose-dependent manner. Especially, the antivirus activity of Xiyanping Injection is superior to ribavirin injection in the early and late stage of virus infection. Attractively, Xiyanping can reduce the viral load in organs and sera, and improve the survival rate of experimental mice. Conclusion Xiyanping Injection, as a traditional Chinese herba. displayed obviously antivirus activity and wide safety range in vitro and in vivo . Our results indicate Xiyanping Injection might be a new candidate of antiviral drug for DENV infection. 摘要：目的 分别通过体外和体内实验研究喜炎平注射液对H型登革病毒( Dengue virus serotype 2, DENV2)的抗病 毒作用。 方法 体外实验以利巴韦林注射液作为阳性对照药物, 分别以灭活、抑制进人、治疗性给药以及预防联合维 持治疗的方式处理人肝癌细胞株HepG2细胞, 在不同时相点收获上清和细胞, 分别利用间接免疫荧光(indirect immunofluorescence assay, IFA)和实时荧光定量PCR(quantitative real-time PCR, qPCR)检测DENV2的病毒载量, 从而评价喜炎 平注射液的体外抗DENV2作用。体内实验以SCID小鼠为实验鼠, 腹腔注射HepG2细胞, 建立模型小鼠, 腹腔注射感染 DENV2,次日给予不同剂量(1、2、4mg/只）的喜炎平注射液, 连续给药4d,部分小鼠于感染第6日取材, 利用IFA检测实 验鼠脾、肝及血清中病毒载量, 并观察实验鼠的生存率。 结果 体外实验显示, 喜炎平注射液能够灭活DENV2,并抑制 DENV2进入细胞, 同时无论是治疗还是治疗联合预防给药, 均可对DENV2的复制具有良好的抑制作用, 即在病毒感染 早期及晚期, 喜炎平均可发挥抗病毒作用, 且优于利巴韦林。进一步在体内实验中同样显示喜炎平具有较好的抗病毒 作用, 可以降低实验鼠脾、肝及血清中病毒载量, 并可提高实验鼠的生存率。 结论 喜炎平注射液具有明显的抗 DENV2病毒的作用 , 可为DENV感染的治疗提供新的候选药物
{"title":"Antivirus activity of Xiyanping Injection against Dengue virus serotype 2 in vitro and in vivo","authors":"Fan Dongying, Gao Na, Jiang Chunhong, Wu Na","doi":"10.13604/J.CNKI.46-1064/R.2020.12.05","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.05","url":null,"abstract":"Objective To evaluate the antivirus activity of Xiyanping Injection against Dengue virus serotype 2 (DENV2) in vitro and in vivo. Methods A human hepatoma cell line HepG2 was treated with Xiyanping Injection through different ways, including the direct inactivation of virus, the inhibition of virus entry, administration after virus exposure and combination of pre- and post-exposure administration. The supernatant and cells were harvested in different time point. Then the virus antigen positive cells were detected by indirect immunofluorescence assay (IFA), and the viral RNA was measured by quantitative real-time PCR (qPCR). Moreover, antiviral activity of Xiyanping Injection was further detected with the HepG2 transplanted SCID mice. The mice were intraperitoneally injected with different doses (1,2,4 mg/mouse) of Xiyanping Injection for 4 days following the challenge of DENV2. The organs and sera in different groups were sampled on the sixth day post infection. Then, the numbers of virus antigen positive cells in spleen, liver and sera of mice were detected by IFA, and the survival rate of each group was monitored for 14 days. Results Xiyanping Injection can inactivate DENV2 and inhibit the entry of DENV2 into cells. The replication of DENV2 was significantly inhibited by Xiyanping Injection with the aforementioned medication in a dose-dependent manner. Especially, the antivirus activity of Xiyanping Injection is superior to ribavirin injection in the early and late stage of virus infection. Attractively, Xiyanping can reduce the viral load in organs and sera, and improve the survival rate of experimental mice. Conclusion Xiyanping Injection, as a traditional Chinese herba. displayed obviously antivirus activity and wide safety range in vitro and in vivo . Our results indicate Xiyanping Injection might be a new candidate of antiviral drug for DENV infection. 摘要：目的 分别通过体外和体内实验研究喜炎平注射液对H型登革病毒( Dengue virus serotype 2, DENV2)的抗病 毒作用。 方法 体外实验以利巴韦林注射液作为阳性对照药物, 分别以灭活、抑制进人、治疗性给药以及预防联合维 持治疗的方式处理人肝癌细胞株HepG2细胞, 在不同时相点收获上清和细胞, 分别利用间接免疫荧光(indirect immunofluorescence assay, IFA)和实时荧光定量PCR(quantitative real-time PCR, qPCR)检测DENV2的病毒载量, 从而评价喜炎 平注射液的体外抗DENV2作用。体内实验以SCID小鼠为实验鼠, 腹腔注射HepG2细胞, 建立模型小鼠, 腹腔注射感染 DENV2,次日给予不同剂量(1、2、4mg/只）的喜炎平注射液, 连续给药4d,部分小鼠于感染第6日取材, 利用IFA检测实 验鼠脾、肝及血清中病毒载量, 并观察实验鼠的生存率。 结果 体外实验显示, 喜炎平注射液能够灭活DENV2,并抑制 DENV2进入细胞, 同时无论是治疗还是治疗联合预防给药, 均可对DENV2的复制具有良好的抑制作用, 即在病毒感染 早期及晚期, 喜炎平均可发挥抗病毒作用, 且优于利巴韦林。进一步在体内实验中同样显示喜炎平具有较好的抗病毒 作用, 可以降低实验鼠脾、肝及血清中病毒载量, 并可提高实验鼠的生存率。 结论 喜炎平注射液具有明显的抗 DENV2病毒的作用 , 可为DENV感染的治疗提供新的候选药物","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81207247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.21
L. Dong-xia, Liu Da-feng, Liu Ya-ling, Zeng Yilan, Zhao Ben-nan, He Wei, Liang Qingfeng
Summarizing the clinical characteristics and the process of two times of nucleic acid turning re-positive after discharge of an imported COVID-19 case in Chengdu in order to provide experience for clinical management of such cases. A retrospective research method was used to analyze the diagnosis and treatment process, as well as the clinical symptoms and examination results 15 days after discharge of an imported COVID-19 case in Chengdu. The case was tested positive for SARS-CoV-2 nucleic acid in Thailand on March 7, 2020. After arriving in Chengdu on March 10, 2020, he was sent to Chengdu Public Health Clinical Medical Center for isolation treatment. On March 11, the SARS-CoV-2 nucleic acid test of nasopharyngeal swab was suspiciously positive and the result of anal swab was positive. The case was admitted to the hospital as an asymptomatic accompanied by a decline in cellular immune function, his physical examination showed no positive signs. Then he was converted to a confirmed case after 22 days of hospitalization. After the case was discharged 15 days from the hospital, his virus nucleic acid had returned re-positive for 2 times. He was accompanied by neurological symptoms and was diagnosed as anxious and depressive when nucleic acid returned re-positive for the second time, and his symptoms resolved after treatment with psychiatric drugs.Asymptomatic patients of COVID-19 can be converted to confirmed cases. Nucleic acid returning re-positive does not mean that the patient's condition has relapsed or worsened. Patients with COVID-19 may have mental disorder, medical staff need to find it out in time and provide psychological intervention or necessary medication. 摘要： 总结成都市一例输人型新型冠状病毒肺炎(coronavirus disease 2019, COVID-19)病例的临床特点及出院后2 次新冠病毒核酸检测阳性的过程,为临床管理此类病例提供经验。回顾性分析成都市一例输人型COVID-19病例的诊 疗过程及出院15d后复诊时的临床症状及检査结果。调査结果显示,病例于2020年3月7日在泰国査SARS-CoV-2 核酸阳性,2020年3月10日抵蓉,由机场送人成都市公共卫生临床医疗中心隔离治疗。3月11日鼻咽拭子新型 冠状病毒核酸检测可疑阳性,肛拭子査新型冠状病毒核酸阳性。体格检査无阳性体征,以“新型冠状病毒肺炎无 症状感染者”收人院治疗,伴有细胞免疫功能下降,在住院22 d(病程第25天)后转为确诊病例。出院后15 d复査新冠 病毒核酸复阳,再次治疗出院后病毒核酸第二次复阳。病例在第二次核酸复阳时伴有神经系统症状,完善检査后排除 颅内感染及占位病变,诊断为焦虑抑郁状态,予精神科药物治疗后症状缓解。COVID-19无症状感染者可转为确诊病 例,核酸复阳不代表患者病情复发或加重。COVID-19患者可出现心理障碍,医务人员需及时发现并予以心理干预及 必要的药物治疗。
{"title":"Diagnosis, treatment and the reasons for re-detectable positive RNA test of one case of imported COVID-19 in Chengdu","authors":"L. Dong-xia, Liu Da-feng, Liu Ya-ling, Zeng Yilan, Zhao Ben-nan, He Wei, Liang Qingfeng","doi":"10.13604/J.CNKI.46-1064/R.2020.12.21","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.21","url":null,"abstract":"Summarizing the clinical characteristics and the process of two times of nucleic acid turning re-positive after discharge of an imported COVID-19 case in Chengdu in order to provide experience for clinical management of such cases. A retrospective research method was used to analyze the diagnosis and treatment process, as well as the clinical symptoms and examination results 15 days after discharge of an imported COVID-19 case in Chengdu. The case was tested positive for SARS-CoV-2 nucleic acid in Thailand on March 7, 2020. After arriving in Chengdu on March 10, 2020, he was sent to Chengdu Public Health Clinical Medical Center for isolation treatment. On March 11, the SARS-CoV-2 nucleic acid test of nasopharyngeal swab was suspiciously positive and the result of anal swab was positive. The case was admitted to the hospital as an asymptomatic accompanied by a decline in cellular immune function, his physical examination showed no positive signs. Then he was converted to a confirmed case after 22 days of hospitalization. After the case was discharged 15 days from the hospital, his virus nucleic acid had returned re-positive for 2 times. He was accompanied by neurological symptoms and was diagnosed as anxious and depressive when nucleic acid returned re-positive for the second time, and his symptoms resolved after treatment with psychiatric drugs.Asymptomatic patients of COVID-19 can be converted to confirmed cases. Nucleic acid returning re-positive does not mean that the patient's condition has relapsed or worsened. Patients with COVID-19 may have mental disorder, medical staff need to find it out in time and provide psychological intervention or necessary medication. 摘要： 总结成都市一例输人型新型冠状病毒肺炎(coronavirus disease 2019, COVID-19)病例的临床特点及出院后2 次新冠病毒核酸检测阳性的过程,为临床管理此类病例提供经验。回顾性分析成都市一例输人型COVID-19病例的诊 疗过程及出院15d后复诊时的临床症状及检査结果。调査结果显示,病例于2020年3月7日在泰国査SARS-CoV-2 核酸阳性,2020年3月10日抵蓉,由机场送人成都市公共卫生临床医疗中心隔离治疗。3月11日鼻咽拭子新型 冠状病毒核酸检测可疑阳性,肛拭子査新型冠状病毒核酸阳性。体格检査无阳性体征,以“新型冠状病毒肺炎无 症状感染者”收人院治疗,伴有细胞免疫功能下降,在住院22 d(病程第25天)后转为确诊病例。出院后15 d复査新冠 病毒核酸复阳,再次治疗出院后病毒核酸第二次复阳。病例在第二次核酸复阳时伴有神经系统症状,完善检査后排除 颅内感染及占位病变,诊断为焦虑抑郁状态,予精神科药物治疗后症状缓解。COVID-19无症状感染者可转为确诊病 例,核酸复阳不代表患者病情复发或加重。COVID-19患者可出现心理障碍,医务人员需及时发现并予以心理干预及 必要的药物治疗。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82120488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.10
C. Jun, Yin Zongbao, Wang Wenhu
Objective To investigate the psychological changes of 84 patients with common type of coronavirus disease 2019 (COVID-19) in Jingzhou City, Hubei, and we aim to guide their psychological rehabilitation training. Methods Using the general condition questionnaire, generalized anxiety scale and depression scale, 84 patients with common COVID-19 admitted to the Second People's Hospital of Jingzhou City in Hubei from January 28th to February 10th, 2020, were investigated by questionnaires at the time of admission, discharge, and on the 14th and 28th day after discharge. The disordered Logistic regression model was used for statistical analysis. Results The incidence of mild, moderate and severe anxiety was respectively 39.29%, 36.90% and 17.86%; The 28 days after discharge were 14.29%, 2.38% and 2.38%, respectively. The incidence rates of mild, moderate, moderate-severe and severe depression were 45.24%, 32.14%, 14.29% and 7.14%, respectively, at the time of diagnosis; and 19.05%, 4.76%, 1.19% and 1.19%, respectively, at the 28th day after discharge. The results of multi-factor analysis showed that the anxiety of different degrees was easy to appear in primary and secondary schools, migrant workers and their families, especially migrant workers. Female, age, family workers were prone to depression, persons with primary and secondary school education level and family workers were prone to mild and moderate-severe depression, especially we should pay attention to the people with university education level. Conclusion COVID-19 patients are with different degrees of anxiety and depression psychological problems, and the factors affecting different degrees of anxiety and depression are not the same, community workers and medical staff should strengthen the psychological rehabilitation treatment of patients after discharge, as soon as possible to make patients recover a healthy psychological state. 摘要：目的 探讨湖北荆州市84例普通型新型冠状病毒肺炎(简称新冠肺炎)患者的心理变化特征, 指导患者心理 康复训练。 方法 使用一般情况调査表、广泛性焦虑量表和抑郁量表, 对湖北荆州市第二人民医院2020年1月28日至 2020年2月10日收治的84名普通型新冠肺炎患者进行人院时、出院时、出院后第14天和第28天问卷调査。运用无序 多分类Logistic回归模型进行统计分析。 结果 84例新冠肺炎患者确诊时轻度、中度和重度焦虑发生率分别是 39.29%、36.90%、17.86%;出院后第28天分别为14.29%、2.38%、2.38%;确诊时抑郁症轻度、中度、中重度及重度发生率 分别是45.24%、32.14%、14.29%、7.14%, 出院后第28天分别为19.05%、4.76%、1.19%、1.19%。多因素分析结果显示:初 中及以下和进城打工者以及家庭工作者易出现不同程度的焦虑情绪, 尤其是进城打工者。女性、年龄、家庭工作者容 易出现抑郁症, 受初中及以下教育者和家庭工作者容易患轻度和中重度抑郁症, 尤其应重视受大学教育者。 结论 新 冠肺炎患者易出现不同程度的焦虑及抑郁心理问题, 且不同程度焦虑和抑郁情绪的影响因素不完全相同, 社区工作者 和医务人员应该加强患者出院后的心理康复治疗, 让患者尽早恢复健康的心理状态
{"title":"Psychological changes of 84 patients with common type of COVID-19 in Jingzhou, Hubei","authors":"C. Jun, Yin Zongbao, Wang Wenhu","doi":"10.13604/J.CNKI.46-1064/R.2020.12.10","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.10","url":null,"abstract":"Objective To investigate the psychological changes of 84 patients with common type of coronavirus disease 2019 (COVID-19) in Jingzhou City, Hubei, and we aim to guide their psychological rehabilitation training. Methods Using the general condition questionnaire, generalized anxiety scale and depression scale, 84 patients with common COVID-19 admitted to the Second People's Hospital of Jingzhou City in Hubei from January 28th to February 10th, 2020, were investigated by questionnaires at the time of admission, discharge, and on the 14th and 28th day after discharge. The disordered Logistic regression model was used for statistical analysis. Results The incidence of mild, moderate and severe anxiety was respectively 39.29%, 36.90% and 17.86%; The 28 days after discharge were 14.29%, 2.38% and 2.38%, respectively. The incidence rates of mild, moderate, moderate-severe and severe depression were 45.24%, 32.14%, 14.29% and 7.14%, respectively, at the time of diagnosis; and 19.05%, 4.76%, 1.19% and 1.19%, respectively, at the 28th day after discharge. The results of multi-factor analysis showed that the anxiety of different degrees was easy to appear in primary and secondary schools, migrant workers and their families, especially migrant workers. Female, age, family workers were prone to depression, persons with primary and secondary school education level and family workers were prone to mild and moderate-severe depression, especially we should pay attention to the people with university education level. Conclusion COVID-19 patients are with different degrees of anxiety and depression psychological problems, and the factors affecting different degrees of anxiety and depression are not the same, community workers and medical staff should strengthen the psychological rehabilitation treatment of patients after discharge, as soon as possible to make patients recover a healthy psychological state. 摘要：目的 探讨湖北荆州市84例普通型新型冠状病毒肺炎(简称新冠肺炎)患者的心理变化特征, 指导患者心理 康复训练。 方法 使用一般情况调査表、广泛性焦虑量表和抑郁量表, 对湖北荆州市第二人民医院2020年1月28日至 2020年2月10日收治的84名普通型新冠肺炎患者进行人院时、出院时、出院后第14天和第28天问卷调査。运用无序 多分类Logistic回归模型进行统计分析。 结果 84例新冠肺炎患者确诊时轻度、中度和重度焦虑发生率分别是 39.29%、36.90%、17.86%;出院后第28天分别为14.29%、2.38%、2.38%;确诊时抑郁症轻度、中度、中重度及重度发生率 分别是45.24%、32.14%、14.29%、7.14%, 出院后第28天分别为19.05%、4.76%、1.19%、1.19%。多因素分析结果显示:初 中及以下和进城打工者以及家庭工作者易出现不同程度的焦虑情绪, 尤其是进城打工者。女性、年龄、家庭工作者容 易出现抑郁症, 受初中及以下教育者和家庭工作者容易患轻度和中重度抑郁症, 尤其应重视受大学教育者。 结论 新 冠肺炎患者易出现不同程度的焦虑及抑郁心理问题, 且不同程度焦虑和抑郁情绪的影响因素不完全相同, 社区工作者 和医务人员应该加强患者出院后的心理康复治疗, 让患者尽早恢复健康的心理状态","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"15 1","pages":"1167-1171"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89526456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.14
Liao Zhenhui, L. Ran, Qiao Yanbo, Guo Hui, Lin Yan
Objective To analyze the spatial distribution characteristics of coronavirus disease 2019 (COVID-19) epidemic of Yunnan province, and we provide targeted prevention and control advice. Methods From 23 January to 22 February 2020, geographic information system was established based on the data of COVID-19 epidemic and demographic information from online reporting system, local indication of spatial auto correlation (LISA) were conducted by GeoDa1.14.0 and Mapinfo 15.0 software. Results Except for Diqing and Nujiang in 16 prefectures (cities) of the province, cases were reported in 14 other prefectures (cities). Among them, the confirmed cases were mainly reported in economically developed regions and regions with more developed tourism industry. The epidemic situation of COVID-19 in Yunnan Province first increased sharply and then decreased slowly. There was no statistical difference in global autocorrelation in incidence, but there were some areas aggregated, local clusters appeared only in the early stages of the epidemic. There were 3 L-L clusters (Dehong, Banna and Zhaotong) 1 H-L cluster (Kunming), 4 L-H clusters (Yuxi,Qujing, Chuxiong and Honghe) of early outbreak. Spatial aggregation gradually disappears over time. Conclusion The previous epidemic situation can be prevented and controlled, and there is no space aggregation, but before the epidemic situation is completely extinguished, we still need to improve our alertness, with scientific and correct methods to prevention and control, and avoid the new outbreak and epidemic. 摘要：目的 分析云南省新型冠状病毒肺炎疫情的空间分布特征,为制定云南省疫情防治措施和疫情的有效控制 提供有效参考。 方法 2020年1月23日至2月22日期间,基于全云南省通过疫情信息报告管理系统进行网络报告的 新型冠状病毒肺炎疫情数据和云南省统计局统计的云南省常驻人口信息,导人GeoDa1.14.0和Mapinfo15.0软件,进行 云南省新型冠状病毒肺炎疫情的描述和空间自相关分析。 结果 全省16州（市）除迪庆州和怒江州无病例报告外,其 他14个州（市)均有病例报告 , 其中确诊报告数主要集中在经济较发达的地区和旅游业较发达地区,云南省新型冠状病 毒肺炎疫情出现先急剧升髙后缓慢下降的趋势,发病率全局的空间自相关无统计学差异,但局部有一定的聚集,局部 地区聚集情况仅出现在疫情较早期,疫情早期存在3个L-L聚集区(德宏州、版纳州和昭通市）,1个H-L区（昆明市）,4个L-H区(玉溪市、曲靖市、楚雄州和红河州）,随着时间的推移,空间聚集情况逐渐消失。 结论 目前疫情可防可控, 无空间聚集性 , 但是在疫情完全扑灭前,仍然需要我们提髙警觉性 , 科学防控 , 避免疫情的再燃和空间的聚集。
{"title":"Spatial analysis of COVID-19 epidemic situation of Yunnan","authors":"Liao Zhenhui, L. Ran, Qiao Yanbo, Guo Hui, Lin Yan","doi":"10.13604/J.CNKI.46-1064/R.2020.12.14","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.14","url":null,"abstract":"Objective To analyze the spatial distribution characteristics of coronavirus disease 2019 (COVID-19) epidemic of Yunnan province, and we provide targeted prevention and control advice. Methods From 23 January to 22 February 2020, geographic information system was established based on the data of COVID-19 epidemic and demographic information from online reporting system, local indication of spatial auto correlation (LISA) were conducted by GeoDa1.14.0 and Mapinfo 15.0 software. Results Except for Diqing and Nujiang in 16 prefectures (cities) of the province, cases were reported in 14 other prefectures (cities). Among them, the confirmed cases were mainly reported in economically developed regions and regions with more developed tourism industry. The epidemic situation of COVID-19 in Yunnan Province first increased sharply and then decreased slowly. There was no statistical difference in global autocorrelation in incidence, but there were some areas aggregated, local clusters appeared only in the early stages of the epidemic. There were 3 L-L clusters (Dehong, Banna and Zhaotong) 1 H-L cluster (Kunming), 4 L-H clusters (Yuxi,Qujing, Chuxiong and Honghe) of early outbreak. Spatial aggregation gradually disappears over time. Conclusion The previous epidemic situation can be prevented and controlled, and there is no space aggregation, but before the epidemic situation is completely extinguished, we still need to improve our alertness, with scientific and correct methods to prevention and control, and avoid the new outbreak and epidemic. 摘要：目的 分析云南省新型冠状病毒肺炎疫情的空间分布特征,为制定云南省疫情防治措施和疫情的有效控制 提供有效参考。 方法 2020年1月23日至2月22日期间,基于全云南省通过疫情信息报告管理系统进行网络报告的 新型冠状病毒肺炎疫情数据和云南省统计局统计的云南省常驻人口信息,导人GeoDa1.14.0和Mapinfo15.0软件,进行 云南省新型冠状病毒肺炎疫情的描述和空间自相关分析。 结果 全省16州（市）除迪庆州和怒江州无病例报告外,其 他14个州（市)均有病例报告 , 其中确诊报告数主要集中在经济较发达的地区和旅游业较发达地区,云南省新型冠状病 毒肺炎疫情出现先急剧升髙后缓慢下降的趋势,发病率全局的空间自相关无统计学差异,但局部有一定的聚集,局部 地区聚集情况仅出现在疫情较早期,疫情早期存在3个L-L聚集区(德宏州、版纳州和昭通市）,1个H-L区（昆明市）,4个L-H区(玉溪市、曲靖市、楚雄州和红河州）,随着时间的推移,空间聚集情况逐渐消失。 结论 目前疫情可防可控, 无空间聚集性 , 但是在疫情完全扑灭前,仍然需要我们提髙警觉性 , 科学防控 , 避免疫情的再燃和空间的聚集。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"12 1","pages":"1183-1187"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84308343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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