Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.13
Liu Yinglai, W. Man, Feng Zhifeng, L. Le
Objective In order to obtain the pathogenic spectrum of acute febrile respiratory syndrome virus in Zhongshan City, and we provide scientific basis for disease control and clinical diagnosis and treatment. Methods Four medical institutions in Zhongshan City were selected as sentinel monitoring hospitals to carry out febrile respiratory syndrome monitoring in outpatient clinics from December 2017 to November 2018, and 8 species of respiratory virus were detected by multiple RT-PCR. Results A total of 1 011 samples were tested, and 368 (36.4%) were positive. The positive detection rates of different viruses were respectively: influenza virus (Flu) 14.0%, adenovirus (AdV) 6.3%, rhinovirus(hRV) 5.9%, respiratory syncytial virus(RSV) 4.9%, coronavirus(hCoV) 3.1%, parainfluenza virus (PIV) 2.4%, human metapneumovirus (HMPV) 2.0% and bocavirus (hBoV) 1.2%. Compared to people ≥16 years, virus detection rates were higher in people <16 years.(27.3% VS 44.7%, χ 2 =32.867, P <0.001), and virus infections were more diverse. All 8 kinds of viruses were detected in infants under 5 years old, among which respiratory syncytial virus (9.2%, 37/404) and adenovirus (9.7%, 39/404) had the highest detection rates. Among people aged 6-15 years, the detection rate of influenza virus was the highest (29.8%). Except for parainfluenza virus and bocavirus, the detection rates of other viruses were different in different months. Conclusions The most common pathogens of febrile respiratory syndrome were influenza virus, adenovirus, rhinovirus, respiratory syncytial virus in Zhongshan City; The younger the age group are, the more diverse the virus are;Different pathogens have different infection seasons, but most pathogens are mainly infected in winter. 摘要：目的 获得中山市急性发热呼吸道症候群病毒病原谱, 为疾病防控及临床诊疗提供科学依据。 方法 2017 年12月至2018年11月, 选择中山市4家医疗机构作为哨点监测医院, 在门诊开展季节性流感等发热呼吸道症候群监 测。采用多重RT-PCR方法检测8种呼吸道感染病毒。 结果 累计检测样本1011份 , ; 368份(36.4%)检出病毒阳性。 不同病毒检出阳性率依次为 ： 流感病毒(Flu)14.0%, 腺病毒(AdV)6.3%, 鼻病毒(hRV)5.9%, 呼吸道合胞病毒(RSV) 4.9%, 冠状病毒(hCoV)3.1%, 副流感病毒(PIV)2.4%, 人偏肺病毒(HMPV)2.0%和博卡病毒(hBoV) 1.2%。与16岁及以 上人群相比, 16岁以下人群病毒检出率更髙(27.3% VS 44.7%, χ 2 =32.867, P <0.001), 病毒感染种类更加多样化。5岁及 以下婴幼儿8种病毒均有检出, 其中呼吸道合胞病毒(9.2%, 37/404)、腺病毒(9.7%, 39/404)检出率最髙。6~15岁人群, 流感病毒检出率(29.8%)最髙。除副流感病毒和博卡病毒外, 其余病毒不同月份检出率存在差异。 结论 流感病毒、腺 病毒、鼻病毒和呼吸道合胞病毒是中山市发热呼吸道症候群的主要病原体;年龄组越小, 病毒多样性越明显;不同病原 体其感染季节不尽相同, 但多数病原体以冬季流行感染为主。
{"title":"Viral etiologies of acute febrile respiratory syndrome in Zhongshan, Guangdong","authors":"Liu Yinglai, W. Man, Feng Zhifeng, L. Le","doi":"10.13604/J.CNKI.46-1064/R.2020.12.13","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.13","url":null,"abstract":"Objective In order to obtain the pathogenic spectrum of acute febrile respiratory syndrome virus in Zhongshan City, and we provide scientific basis for disease control and clinical diagnosis and treatment. Methods Four medical institutions in Zhongshan City were selected as sentinel monitoring hospitals to carry out febrile respiratory syndrome monitoring in outpatient clinics from December 2017 to November 2018, and 8 species of respiratory virus were detected by multiple RT-PCR. Results A total of 1 011 samples were tested, and 368 (36.4%) were positive. The positive detection rates of different viruses were respectively: influenza virus (Flu) 14.0%, adenovirus (AdV) 6.3%, rhinovirus(hRV) 5.9%, respiratory syncytial virus(RSV) 4.9%, coronavirus(hCoV) 3.1%, parainfluenza virus (PIV) 2.4%, human metapneumovirus (HMPV) 2.0% and bocavirus (hBoV) 1.2%. Compared to people ≥16 years, virus detection rates were higher in people <16 years.(27.3% VS 44.7%, χ 2 =32.867, P <0.001), and virus infections were more diverse. All 8 kinds of viruses were detected in infants under 5 years old, among which respiratory syncytial virus (9.2%, 37/404) and adenovirus (9.7%, 39/404) had the highest detection rates. Among people aged 6-15 years, the detection rate of influenza virus was the highest (29.8%). Except for parainfluenza virus and bocavirus, the detection rates of other viruses were different in different months. Conclusions The most common pathogens of febrile respiratory syndrome were influenza virus, adenovirus, rhinovirus, respiratory syncytial virus in Zhongshan City; The younger the age group are, the more diverse the virus are;Different pathogens have different infection seasons, but most pathogens are mainly infected in winter. 摘要：目的 获得中山市急性发热呼吸道症候群病毒病原谱, 为疾病防控及临床诊疗提供科学依据。 方法 2017 年12月至2018年11月, 选择中山市4家医疗机构作为哨点监测医院, 在门诊开展季节性流感等发热呼吸道症候群监 测。采用多重RT-PCR方法检测8种呼吸道感染病毒。 结果 累计检测样本1011份 , ; 368份(36.4%)检出病毒阳性。 不同病毒检出阳性率依次为 ： 流感病毒(Flu)14.0%, 腺病毒(AdV)6.3%, 鼻病毒(hRV)5.9%, 呼吸道合胞病毒(RSV) 4.9%, 冠状病毒(hCoV)3.1%, 副流感病毒(PIV)2.4%, 人偏肺病毒(HMPV)2.0%和博卡病毒(hBoV) 1.2%。与16岁及以 上人群相比, 16岁以下人群病毒检出率更髙(27.3% VS 44.7%, χ 2 =32.867, P <0.001), 病毒感染种类更加多样化。5岁及 以下婴幼儿8种病毒均有检出, 其中呼吸道合胞病毒(9.2%, 37/404)、腺病毒(9.7%, 39/404)检出率最髙。6~15岁人群, 流感病毒检出率(29.8%)最髙。除副流感病毒和博卡病毒外, 其余病毒不同月份检出率存在差异。 结论 流感病毒、腺 病毒、鼻病毒和呼吸道合胞病毒是中山市发热呼吸道症候群的主要病原体;年龄组越小, 病毒多样性越明显;不同病原 体其感染季节不尽相同, 但多数病原体以冬季流行感染为主。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82034495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To explore whether different drainage methods have positive effects on HIV positive patients with c:ryptoc:oc:c:al meningitis (CM). Methods From January 2017 to December 2019, 40 patients with HIV positive CM were treated in the Department of Infectious Diseases of the First Affiliated Hospital of Guangxi Medical University and Nanning Infectious Diseases Hospital Affiliated to Guangxi Medical University. 20 patients with continuous lumbar drainage were selected as the observation group and 20 patients with regular lumbar puncture drainage were enrolled into the control group. The clinical manifestations, CD4 count, cerebrospinal fluid examination and cryptococcal ink staining results of patients before and after treatment were recorded. Results There was no significant difference in general data between the two groups before treatment ( P >0.05). After treatment, the white blood cell count [(28.70±12.04)x10 6 /L vs (68.80±34.71) x10 6 /L] and protein content [(477.25±198.61) mg/L vs (712.15±427.89) mg/L] in cerebrospinal fluid decreased significantly in the observation group, and similar results were found in the control group ( P 0.05)。治疗后观察组中脑脊液白细胞计数[(28.70±12.04)x10 6 /L]、蛋白含量[(477.25±198.61) mg/L]比治疗前[(68.80±34.71)x10 6 /L、（712.15±427.89) mg/L]明显下降( P <0.001), 对照组中也有相似的结果( P <0.05), 治疗后两组患者脑脊液的CD4计数[观察组(31.30±22.00)cells/μL、对照组(27.70±20.18)cells/μL]、糖含量[观察组(3.41±0.62)mmol/L、对照组（2.80±0.77) mmol/L]、氯化物[观察组（127.78±9.47)mmol/L、对照组（125.25±8.03) mmol/L]均较治疗 前升髙( P <0.05)。治疗后两组患者间CD4/CD8、白细胞计数、糖含量差异有统计学意义( P <0.05)。治疗后观察组头痛、恶心/呕吐症状较对照组改善更明显。最终 , 腰椎持续引流方式治疗有效15例, 无效5例;定期腰椎穿刺引流治疗7例 有效。 结论 腰椎持续引流方式治疗隐球菌性脑膜炎, 能较快缓解临床症状, 加快脑脊液的更新, 缩短病原菌清除时 间, 值得临床推广应用
{"title":"Effect of lumbar drainage on HIV-positive cryptococcal meningitis","authors":"Luan He, WU Ze-zhou, Qin Jianglong, X. Fang, Wang Peiqi, Liao Bo-ming","doi":"10.13604/J.CNKI.46-1064/R.2020.12.08","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.08","url":null,"abstract":"Objective To explore whether different drainage methods have positive effects on HIV positive patients with c:ryptoc:oc:c:al meningitis (CM). Methods From January 2017 to December 2019, 40 patients with HIV positive CM were treated in the Department of Infectious Diseases of the First Affiliated Hospital of Guangxi Medical University and Nanning Infectious Diseases Hospital Affiliated to Guangxi Medical University. 20 patients with continuous lumbar drainage were selected as the observation group and 20 patients with regular lumbar puncture drainage were enrolled into the control group. The clinical manifestations, CD4 count, cerebrospinal fluid examination and cryptococcal ink staining results of patients before and after treatment were recorded. Results There was no significant difference in general data between the two groups before treatment ( P >0.05). After treatment, the white blood cell count [(28.70±12.04)x10 6 /L vs (68.80±34.71) x10 6 /L] and protein content [(477.25±198.61) mg/L vs (712.15±427.89) mg/L] in cerebrospinal fluid decreased significantly in the observation group, and similar results were found in the control group ( P 0.05)。治疗后观察组中脑脊液白细胞计数[(28.70±12.04)x10 6 /L]、蛋白含量[(477.25±198.61) mg/L]比治疗前[(68.80±34.71)x10 6 /L、（712.15±427.89) mg/L]明显下降( P <0.001), 对照组中也有相似的结果( P <0.05), 治疗后两组患者脑脊液的CD4计数[观察组(31.30±22.00)cells/μL、对照组(27.70±20.18)cells/μL]、糖含量[观察组(3.41±0.62)mmol/L、对照组（2.80±0.77) mmol/L]、氯化物[观察组（127.78±9.47)mmol/L、对照组（125.25±8.03) mmol/L]均较治疗 前升髙( P <0.05)。治疗后两组患者间CD4/CD8、白细胞计数、糖含量差异有统计学意义( P <0.05)。治疗后观察组头痛、恶心/呕吐症状较对照组改善更明显。最终 , 腰椎持续引流方式治疗有效15例, 无效5例;定期腰椎穿刺引流治疗7例 有效。 结论 腰椎持续引流方式治疗隐球菌性脑膜炎, 能较快缓解临床症状, 加快脑脊液的更新, 缩短病原菌清除时 间, 值得临床推广应用","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80854536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.01
Li Rongjian, Lan Xueni, Shen Zhiyong, Lan Guanghua, Tang Shuai
Objective To understand the prevalence rates and the related risk factors of HIV, HCV and HIV/HCV coinfection in drug users in the Sino-Vietnamese border areas of Guangxi, and we provide reference for high-risk intervention in this population. Methods From April to June every year from 2012 to 2019, a questionnaire developed by the state was used to carry out continuous cross-sectional surveys on the post surveillance sites in Guangxi border areas, community drug addicts and morphine urine test positive persons in methadone clinics in the past month, and blood samples were taken from them for the detections of HIV and hepatitis C virus (HCV) antibodies. Results A total of 12 842 drug users were monitored from 2012 to 2019. The overall positive rates of HIV, HCV and HIV/HCV co-infection were 3.84%, 43.34% and 3.14%, respectively. HIV, HCV and HIV/HCV co-infection rates showed an decrease trend year by year. The prevalence differed in drug users with different demographic characteristics. Logistic regression analysis showed that HIV infection related risk factors included being female, primary education and injecting drug use. HCV infection related risk factors included 20 years old or more, divorced or widowed, minorities other than Han and Zhuang, being traditional drug user or mixed drug user, and injecting drug use. HIV/HCV co-infection related risk factors included being female, primary education and injecting drug use. Conclusion The HIV infection rate and HCV antibody positive rate in drug users in border areas of Guangxi were relatively high, and the infection rate of HIV/HCV combined infection were low. The use of novel type drugs was in increase. Targeted effective interventions should be conducted in female and older drug users as well as drug users having a history of injection drug use. 摘要:目的 了解广西中越边境地区吸毒人群中HIV、丙肝病毒(HCV)及HIV/HCV合并感染情况及其影响因素, 为在该人群中开展髙危行为干预提供参考依据。 方法 2012—2019年间每年4一6月, 采用国家统一制定的问卷对广 西中越边境哨点监管场所、社区和美沙酮门诊最近1个月吗啡尿检阳性者开展连续横断面调査, 并采集调査对象血液 标本进行HIV和HCV抗体检测。 结果 2012—2019年共监测12 842名毒品使用者。HIV感染率为3.84%, HCV抗体 阳性率为43.34%, HIV/HCV合并感染率为3.14%。HIV、HCV及HIV/HCV合并感染率整体上呈逐年下降的趋势。不同 行为特征的吸毒人群感染情况不同。Logistic回归分析显示, 女性、小学学历、有注射吸毒史是感染HIV的危险因素。年龄>20岁、离异或丧偶、除汉族和壮族外的其他少数民族、传统毒品和混合毒品使用者、有注射毒品史是感染HCV的 危险因素。而女性、小学文化、有注射毒品史是HIV/HCV合并感染的危险因素。 结论 广西边境吸毒人群整体HIV感 染率和HCV抗体阳性率较髙, HIV/HCV合并感染率较低, 新型毒品使用者比例呈上升趋势。提示需针对女性、髙年龄 组及有注射吸毒史的吸毒人群开展有效干预措施。
{"title":"HIV and HCV infection status and related risk factors in drug users at the Sino-Vietnamese border in Guangxi, 2012 — 2019","authors":"Li Rongjian, Lan Xueni, Shen Zhiyong, Lan Guanghua, Tang Shuai","doi":"10.13604/J.CNKI.46-1064/R.2020.12.01","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.01","url":null,"abstract":"Objective To understand the prevalence rates and the related risk factors of HIV, HCV and HIV/HCV coinfection in drug users in the Sino-Vietnamese border areas of Guangxi, and we provide reference for high-risk intervention in this population. Methods From April to June every year from 2012 to 2019, a questionnaire developed by the state was used to carry out continuous cross-sectional surveys on the post surveillance sites in Guangxi border areas, community drug addicts and morphine urine test positive persons in methadone clinics in the past month, and blood samples were taken from them for the detections of HIV and hepatitis C virus (HCV) antibodies. Results A total of 12 842 drug users were monitored from 2012 to 2019. The overall positive rates of HIV, HCV and HIV/HCV co-infection were 3.84%, 43.34% and 3.14%, respectively. HIV, HCV and HIV/HCV co-infection rates showed an decrease trend year by year. The prevalence differed in drug users with different demographic characteristics. Logistic regression analysis showed that HIV infection related risk factors included being female, primary education and injecting drug use. HCV infection related risk factors included 20 years old or more, divorced or widowed, minorities other than Han and Zhuang, being traditional drug user or mixed drug user, and injecting drug use. HIV/HCV co-infection related risk factors included being female, primary education and injecting drug use. Conclusion The HIV infection rate and HCV antibody positive rate in drug users in border areas of Guangxi were relatively high, and the infection rate of HIV/HCV combined infection were low. The use of novel type drugs was in increase. Targeted effective interventions should be conducted in female and older drug users as well as drug users having a history of injection drug use. 摘要:目的 了解广西中越边境地区吸毒人群中HIV、丙肝病毒(HCV)及HIV/HCV合并感染情况及其影响因素, 为在该人群中开展髙危行为干预提供参考依据。 方法 2012—2019年间每年4一6月, 采用国家统一制定的问卷对广 西中越边境哨点监管场所、社区和美沙酮门诊最近1个月吗啡尿检阳性者开展连续横断面调査, 并采集调査对象血液 标本进行HIV和HCV抗体检测。 结果 2012—2019年共监测12 842名毒品使用者。HIV感染率为3.84%, HCV抗体 阳性率为43.34%, HIV/HCV合并感染率为3.14%。HIV、HCV及HIV/HCV合并感染率整体上呈逐年下降的趋势。不同 行为特征的吸毒人群感染情况不同。Logistic回归分析显示, 女性、小学学历、有注射吸毒史是感染HIV的危险因素。年龄>20岁、离异或丧偶、除汉族和壮族外的其他少数民族、传统毒品和混合毒品使用者、有注射毒品史是感染HCV的 危险因素。而女性、小学文化、有注射毒品史是HIV/HCV合并感染的危险因素。 结论 广西边境吸毒人群整体HIV感 染率和HCV抗体阳性率较髙, HIV/HCV合并感染率较低, 新型毒品使用者比例呈上升趋势。提示需针对女性、髙年龄 组及有注射吸毒史的吸毒人群开展有效干预措施。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79070941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.07
Yang Chengqing, Mei Chunlin, Cao Tanze, Z. Meng, Chen Shufang, Du Ronghui
Objective To explore the diagnostic efficacy of clinical decision tree and integral model in tuberculous pleurisy. Methods A prospective randomized controlled study was conducted in patients with pleural effusion admitted to Wuhan Pulmonary Hospital from January 1 to June 30, 2019. Patients with pleural effusion were randomly enrolled into the clinical decision tree group and the integral model group. Two kinds of comprehensive diagnosis models were used for diagnosis, and the diagnostic efficacy of the two methods was compared. Results The clinical decision tree group included 127 patients, 90 male, 37 female, 18–88 years old, with an average age of (49.33±19.63) years. Finally, 108 cases of tuberculous pleurisy and 19 cases of non tuberculous pleurisy were diagnosed. In the integral model group, 120 patients were included, including 89 males and 31 females, 13-84 years old, with an average age of (46.41±20.01) years. 94 cases of tuberculous pleurisy and 26 cases of non tuberculous pleurisy were finally diagnosed. The sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy of clinical decision tree method were 57.4%, 78.9%, 93.9%, 24.6% and 60.6% respectively; and of integral model method were 75.5%, 96.2%, 98.6%, 52.1% and 80.0% respectively. Conclusion The sensitivity, specificity and accuracy of the integral model method in the diagnosis of tuberculous pleurisy were higher than that of the clinical decision tree method. The method is simple and can be widely used in the clinical diagnosis of tuberculous pleurisy. 摘要：目的 探讨临床决策树法与积分模型法对结核性胸膜炎的诊断效能。 方法 采用前瞻性随机对照研究方 法, 以2019年1月1日一2019年6月30日武汉市肺科医院收治的胸腔积液患者为研究对象。符合人组标准的胸腔积液 患者随机人组临床决策树法组和积分模型法组, 分别采用两种综合诊断模型进行诊断, 对比两组方法的诊断效能。 结果 临床决策树组共纳人127例患者, 其中男性90例, 女性37例, 年龄18~88岁, 平均(49.33±19.63)岁, 最后诊断结 核性胸膜炎108例, 非结核性胸膜炎19例；积分模型组共纳人120例患者, 其中男性89例, 女性31例, 年龄13 ~ 84岁, 平均(46.41±20.01)岁 , 最后诊断结核性胸膜炎94例 , 非结核性胸膜炎26例。临床决策树法诊断结核性胸膜炎的敏感 度57.4%, 特异度78.9%, 阳性预测值93.9%, 阴性预测值24.6%, 诊断准确率60.6%;积分模型法诊断结核性胸膜炎的敏 感度75.5%, 特异度96.2%, 阳性预测值98.6%, 阴性预测值52.1%, 诊断准确率80.0%。 结论 积分模型法诊断结核性胸 膜炎敏感度、特异度及诊断准确率均髙于临床决策树法, 方法简单, 可推广应用于结核性胸膜炎的临床诊断
{"title":"Diagnostic efficacy of clinical decision tree and integral model in tuberculous pleurisy","authors":"Yang Chengqing, Mei Chunlin, Cao Tanze, Z. Meng, Chen Shufang, Du Ronghui","doi":"10.13604/J.CNKI.46-1064/R.2020.12.07","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.07","url":null,"abstract":"Objective To explore the diagnostic efficacy of clinical decision tree and integral model in tuberculous pleurisy. Methods A prospective randomized controlled study was conducted in patients with pleural effusion admitted to Wuhan Pulmonary Hospital from January 1 to June 30, 2019. Patients with pleural effusion were randomly enrolled into the clinical decision tree group and the integral model group. Two kinds of comprehensive diagnosis models were used for diagnosis, and the diagnostic efficacy of the two methods was compared. Results The clinical decision tree group included 127 patients, 90 male, 37 female, 18–88 years old, with an average age of (49.33±19.63) years. Finally, 108 cases of tuberculous pleurisy and 19 cases of non tuberculous pleurisy were diagnosed. In the integral model group, 120 patients were included, including 89 males and 31 females, 13-84 years old, with an average age of (46.41±20.01) years. 94 cases of tuberculous pleurisy and 26 cases of non tuberculous pleurisy were finally diagnosed. The sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy of clinical decision tree method were 57.4%, 78.9%, 93.9%, 24.6% and 60.6% respectively; and of integral model method were 75.5%, 96.2%, 98.6%, 52.1% and 80.0% respectively. Conclusion The sensitivity, specificity and accuracy of the integral model method in the diagnosis of tuberculous pleurisy were higher than that of the clinical decision tree method. The method is simple and can be widely used in the clinical diagnosis of tuberculous pleurisy. 摘要：目的 探讨临床决策树法与积分模型法对结核性胸膜炎的诊断效能。 方法 采用前瞻性随机对照研究方 法, 以2019年1月1日一2019年6月30日武汉市肺科医院收治的胸腔积液患者为研究对象。符合人组标准的胸腔积液 患者随机人组临床决策树法组和积分模型法组, 分别采用两种综合诊断模型进行诊断, 对比两组方法的诊断效能。 结果 临床决策树组共纳人127例患者, 其中男性90例, 女性37例, 年龄18~88岁, 平均(49.33±19.63)岁, 最后诊断结 核性胸膜炎108例, 非结核性胸膜炎19例；积分模型组共纳人120例患者, 其中男性89例, 女性31例, 年龄13 ~ 84岁, 平均(46.41±20.01)岁 , 最后诊断结核性胸膜炎94例 , 非结核性胸膜炎26例。临床决策树法诊断结核性胸膜炎的敏感 度57.4%, 特异度78.9%, 阳性预测值93.9%, 阴性预测值24.6%, 诊断准确率60.6%;积分模型法诊断结核性胸膜炎的敏 感度75.5%, 特异度96.2%, 阳性预测值98.6%, 阴性预测值52.1%, 诊断准确率80.0%。 结论 积分模型法诊断结核性胸 膜炎敏感度、特异度及诊断准确率均髙于临床决策树法, 方法简单, 可推广应用于结核性胸膜炎的临床诊断","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82733251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.13604/J.CNKI.46-1064/R.2020.12.18
Rao Jian, Yang Rongxing, Zhu Zi-li, Liu Yang, Chen Peng
Objective To master the disinfection effect of medical institutions at different levels in Shenzhen, and we provide scientific basis for improving the quality of disinfection and reducing hospital-acquired infections. Methods According to the relevant standards, the quality of disinfection in medical institutions in Shenzhen city from 2015 to 2019 was continuously monitored by filed sampling and experimental testing. Results From 2015 to 2019, a total of 139 025 samples were collected form medical institutions in Shenzhen, of which 133 714 samples were qualified, and the qualified rate was 96.18%. The qualified rates for disinfection in 2015-2019 were 96.34%, 95.06%, 95.78%, 96.06%, 97.64%, respectively. The qualified rate increased year by year from 2016, and the difference was statistically significant ( P <0.001). Among all the monitoring items, the qualified rate of therapeutic water was the highest at 100.00%. The qualified rate ultraviolet lamp was the lowest at 91.75%. The qualified rate of private and individual clinics was 95.40%, which was lower than that of primary (98.48%), secondary (98.17%) and tertiary (97.84%) medical institutions ( P <0.05). Conclusion The overall quality of disinfection in medical institutions in Shenzhen is good, but the qualified rate of air, sanitary hand and ultraviolet lamp is low, and qualified rate of disinfection in private and individual clinics varies considerably over a five-year period, requiring further strengthening of supervision and management. 摘要：目的 掌握深圳市各级医疗机构消毒效果,为提髙消毒质量,减少院内感染提供科学依据。 方法 依据相 关标准,通过现场采样和微生物检验方法,对深圳市2015—2019年医疗机构消毒质量进行连续监测。 结果 深圳市 2015—2019年医疗机构共采样139 025件,合格样品133 714件,合格率96.18%。2015—2019年消毒合格率分别为 96.34%、95.06%、95.78%、96.06%、97.64%,从2016年开始合格率逐年上升,差异有统计学意义( P <0.001)。各监测项目 中,治疗用水合格率最髙,为100.00%;紫外线灯合格率最低,为91.75%。私营医疗机构及个体诊所合格率为95.40%, 三级医疗机构消毒合格率为97.84%、二级为98.17%,一级为98.48%,不同级别医疗机构消毒合格率差异有统计学意义 ( P <0.05)。其中,三级医疗机构手卫生和消毒后内镜消毒合格率偏低,私营及个体诊所空气和手卫生消毒合格率最低。 结论 深圳市医疗机构消毒质量整体较好,但空气、手卫生、紫外线灯消毒合格率较低,私营及个体诊所5年内消毒合 格率变化幅度较大,需要进一步加强监督管理。
{"title":"Quality monitoring analysis of disinfection effectiveness in medical institutions in Shenzhen, 2015-2019","authors":"Rao Jian, Yang Rongxing, Zhu Zi-li, Liu Yang, Chen Peng","doi":"10.13604/J.CNKI.46-1064/R.2020.12.18","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.12.18","url":null,"abstract":"Objective To master the disinfection effect of medical institutions at different levels in Shenzhen, and we provide scientific basis for improving the quality of disinfection and reducing hospital-acquired infections. Methods According to the relevant standards, the quality of disinfection in medical institutions in Shenzhen city from 2015 to 2019 was continuously monitored by filed sampling and experimental testing. Results From 2015 to 2019, a total of 139 025 samples were collected form medical institutions in Shenzhen, of which 133 714 samples were qualified, and the qualified rate was 96.18%. The qualified rates for disinfection in 2015-2019 were 96.34%, 95.06%, 95.78%, 96.06%, 97.64%, respectively. The qualified rate increased year by year from 2016, and the difference was statistically significant ( P <0.001). Among all the monitoring items, the qualified rate of therapeutic water was the highest at 100.00%. The qualified rate ultraviolet lamp was the lowest at 91.75%. The qualified rate of private and individual clinics was 95.40%, which was lower than that of primary (98.48%), secondary (98.17%) and tertiary (97.84%) medical institutions ( P <0.05). Conclusion The overall quality of disinfection in medical institutions in Shenzhen is good, but the qualified rate of air, sanitary hand and ultraviolet lamp is low, and qualified rate of disinfection in private and individual clinics varies considerably over a five-year period, requiring further strengthening of supervision and management. 摘要：目的 掌握深圳市各级医疗机构消毒效果,为提髙消毒质量,减少院内感染提供科学依据。 方法 依据相 关标准,通过现场采样和微生物检验方法,对深圳市2015—2019年医疗机构消毒质量进行连续监测。 结果 深圳市 2015—2019年医疗机构共采样139 025件,合格样品133 714件,合格率96.18%。2015—2019年消毒合格率分别为 96.34%、95.06%、95.78%、96.06%、97.64%,从2016年开始合格率逐年上升,差异有统计学意义( P <0.001)。各监测项目 中,治疗用水合格率最髙,为100.00%;紫外线灯合格率最低,为91.75%。私营医疗机构及个体诊所合格率为95.40%, 三级医疗机构消毒合格率为97.84%、二级为98.17%,一级为98.48%,不同级别医疗机构消毒合格率差异有统计学意义 ( P <0.05)。其中,三级医疗机构手卫生和消毒后内镜消毒合格率偏低,私营及个体诊所空气和手卫生消毒合格率最低。 结论 深圳市医疗机构消毒质量整体较好,但空气、手卫生、紫外线灯消毒合格率较低,私营及个体诊所5年内消毒合 格率变化幅度较大,需要进一步加强监督管理。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83270808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.13604/J.CNKI.46-1064/R.2020.11.04
Peng Ping, Chen Mu, P. Hui, Xu Lixian, Deng Xilong, Mo Xiaoneng
Objective To explore and understand the injury degree of human lung induced by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2), through retrospectively analysis of the 47 patients’ pulmonary function in the period of recovery from COVID -19. Methods Totally 47 COVID-19 patients treated in Guangzhou Eighth People′s Hospital from January to February 2020, and isolated 14 days were selected as the research subjects. The detected results of pulmonary function, chest CT, and SARS-CoV-2 antibodies were retrospectively analyzed. Results In the 47 COVID-19 convalescent patients, 40 (85.1%) showed negative for IgM of SARS-CoV-2 and 44 (93.6%) showed positive for IgG, 7 showed positive for both IgM and IgG, and 3 showed negative for both IgM and IgG. 29 cases (61.7%) of pulmonary dysfunction, of which there were 19 mild, 10 moderate, 3 restrictive ventilation dysfunction, 1 obstructive ventilation dysfunction, 2 ventilation dysfunction, 1 mixed ventilation dysfunction, 26 diffusion ventilation dysfunction, 2 mixed diffusion & restrictive ventilation dysfunction, 1 mixed diffusion & obstructive ventilation dysfunction, 2 mixed diffusion & ventilation dysfunction, and 5 small airway dysfunction. 10 cases (90.9%) of pulmonary dysfunction in 11 severe COVID-19 patients, of which 4 were mild and 6 were moderate; 19 cases (52.8%) of pulmonary dysfunction in 36 general COVID-19 patients, of which 15 were mild and 4 were moderate; Comparing the two groups, the cases of pulmonary dysfunction in severe COVID-19 patients were more than general patients. 35 cases of abnormal chest CT in convalescent patients. The abnormal chest CT image mostly showed diffuse large ground glass-like shadows, scattered patchy shadows, and fiber strand shadows; 8 cases showed diffuse large ground glass and fiber strand shadows, of which 7 from moderate pulmonary dysfunction VS 1 from mild pulmonary dysfunction, 6 from severe COVID-19 patients VS 2 from general patients. Conclusion The majority of patients with COVID-19 in the recovery period have pulmonary dysfunction, which mostly manifests as diffuse dysfunction. The exploration is of certain clinical significance for guiding rehabilitation treatment. 摘要：目的 了解 47 例新型冠状病毒肺炎 (COVID-19) 患者恢复期肺功能状况, 分析新型冠状病毒对肺的损伤程 度, 为 COVID-19 致肺损伤恢复提供实验依据。 方法 选取 2020 年 1 月—2 月在广州市第八人民医院治愈出院, 隔离 14 d 后的 47 例 COVID-19 恢复期患者为研究对象, 对检测肺功能、胸部 CT、新型冠状病毒抗体结果进行回顾性分析。 结果 47 例恢复期 COVID-19 患者新型冠状病毒 IgM 阴性 40 例 (占 85.1%) , IgG 阳性 44 例 (占 93.6%) , 其中 IgM、IgG 均 阳性 7 例, IgM、IgG 均阴性 3 例。肺功能损障碍 29 例 (占 61.7%) , 其中轻度、中度障碍分别 19 例、10 例。限制性通气功 能障碍 3 例, 阻塞性通气功能障碍 1 例, 通气功能障碍 2 例, 混合通气功能障碍 1 例, 弥散功能障碍 26 例, 弥散并限制性 通气功能障碍 2 例, 弥散并阻塞性通气功能障碍 1 例, 弥散并通气功能障碍 2 例, 小气道功能障碍共 5 例。11 例重型 COVID-19 患者肺功能障碍 10 例 (占 90.9%) , 其中轻度 4 例, 中度 6 例, 36 例普通型患者肺功能障碍 19 例 (占 52.8%) , 其中 轻度 15 例, 中度 4 例, 两组对比重型 COVID-19 患者中度肺功能障碍较普通型多。恢复期患者胸部 CT 异常 35 例, 多表 现为弥漫大片状磨玻璃样影、散在斑片影、纤维条索影; 其中表现为弥漫大片磨玻璃影和纤维条索影者共 8 例, 中度肺 功能障碍患者 (7 例) 较轻度肺功能障碍患者多 (1 例) , 重型患者 (6 例) 较普通型患者 (2 例) 多。 结论 COVID-19 患者 恢复
{"title":"Pulmonary function of 47 patients with COVID-19 in recovery period in Guangzhou","authors":"Peng Ping, Chen Mu, P. Hui, Xu Lixian, Deng Xilong, Mo Xiaoneng","doi":"10.13604/J.CNKI.46-1064/R.2020.11.04","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.11.04","url":null,"abstract":"Objective To explore and understand the injury degree of human lung induced by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2), through retrospectively analysis of the 47 patients’ pulmonary function in the period of recovery from COVID -19. Methods Totally 47 COVID-19 patients treated in Guangzhou Eighth People′s Hospital from January to February 2020, and isolated 14 days were selected as the research subjects. The detected results of pulmonary function, chest CT, and SARS-CoV-2 antibodies were retrospectively analyzed. Results In the 47 COVID-19 convalescent patients, 40 (85.1%) showed negative for IgM of SARS-CoV-2 and 44 (93.6%) showed positive for IgG, 7 showed positive for both IgM and IgG, and 3 showed negative for both IgM and IgG. 29 cases (61.7%) of pulmonary dysfunction, of which there were 19 mild, 10 moderate, 3 restrictive ventilation dysfunction, 1 obstructive ventilation dysfunction, 2 ventilation dysfunction, 1 mixed ventilation dysfunction, 26 diffusion ventilation dysfunction, 2 mixed diffusion & restrictive ventilation dysfunction, 1 mixed diffusion & obstructive ventilation dysfunction, 2 mixed diffusion & ventilation dysfunction, and 5 small airway dysfunction. 10 cases (90.9%) of pulmonary dysfunction in 11 severe COVID-19 patients, of which 4 were mild and 6 were moderate; 19 cases (52.8%) of pulmonary dysfunction in 36 general COVID-19 patients, of which 15 were mild and 4 were moderate; Comparing the two groups, the cases of pulmonary dysfunction in severe COVID-19 patients were more than general patients. 35 cases of abnormal chest CT in convalescent patients. The abnormal chest CT image mostly showed diffuse large ground glass-like shadows, scattered patchy shadows, and fiber strand shadows; 8 cases showed diffuse large ground glass and fiber strand shadows, of which 7 from moderate pulmonary dysfunction VS 1 from mild pulmonary dysfunction, 6 from severe COVID-19 patients VS 2 from general patients. Conclusion The majority of patients with COVID-19 in the recovery period have pulmonary dysfunction, which mostly manifests as diffuse dysfunction. The exploration is of certain clinical significance for guiding rehabilitation treatment. 摘要：目的 了解 47 例新型冠状病毒肺炎 (COVID-19) 患者恢复期肺功能状况, 分析新型冠状病毒对肺的损伤程 度, 为 COVID-19 致肺损伤恢复提供实验依据。 方法 选取 2020 年 1 月—2 月在广州市第八人民医院治愈出院, 隔离 14 d 后的 47 例 COVID-19 恢复期患者为研究对象, 对检测肺功能、胸部 CT、新型冠状病毒抗体结果进行回顾性分析。 结果 47 例恢复期 COVID-19 患者新型冠状病毒 IgM 阴性 40 例 (占 85.1%) , IgG 阳性 44 例 (占 93.6%) , 其中 IgM、IgG 均 阳性 7 例, IgM、IgG 均阴性 3 例。肺功能损障碍 29 例 (占 61.7%) , 其中轻度、中度障碍分别 19 例、10 例。限制性通气功 能障碍 3 例, 阻塞性通气功能障碍 1 例, 通气功能障碍 2 例, 混合通气功能障碍 1 例, 弥散功能障碍 26 例, 弥散并限制性 通气功能障碍 2 例, 弥散并阻塞性通气功能障碍 1 例, 弥散并通气功能障碍 2 例, 小气道功能障碍共 5 例。11 例重型 COVID-19 患者肺功能障碍 10 例 (占 90.9%) , 其中轻度 4 例, 中度 6 例, 36 例普通型患者肺功能障碍 19 例 (占 52.8%) , 其中 轻度 15 例, 中度 4 例, 两组对比重型 COVID-19 患者中度肺功能障碍较普通型多。恢复期患者胸部 CT 异常 35 例, 多表 现为弥漫大片状磨玻璃样影、散在斑片影、纤维条索影; 其中表现为弥漫大片磨玻璃影和纤维条索影者共 8 例, 中度肺 功能障碍患者 (7 例) 较轻度肺功能障碍患者多 (1 例) , 重型患者 (6 例) 较普通型患者 (2 例) 多。 结论 COVID-19 患者 恢复","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73775645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.13604/J.CNKI.46-1064/R.2020.11.06
Li Tingting, Liu Haiyan, Wang Limei, Li Pinghua
Objective To investigate the occurrence and characteristics of acute poisoning events in the tropical regions of Yunnan Province, we provide evidence for prevention and control measures. Methods A three-level general hospital in typical tropical area of Yunnan Province was selected as the research object. All the cases of acute poisoning during 2013.01.01 to 2018.12.31 were retrieved from the information system to analyze the characteristics of population, the type of poisoning, the season of poisoning event and treatment effect. Results A total of 1 364 cases of acute poisoning were collected during the 6 years. Acute poisoning occurred in all age groups, and 20 to 50 age groups accounted for 60.19% of all cases. On poisoning substances, agricultural chemicals, drugs, herbal medicines and plant poisoning accounted for 48.39%, 16.72% and 11.80% respectively. Among them, paraquat, organophosphorus pesticide, wild mushroom, aconitine and rodenticide were 244, 189, 96, 90, 89 cases respectively, which took the top 5. A wide range of tropical poisonous plants that caused acute poisoning. Almost all the wild mushroom poisoning cases and 57.26% of all the poisoning cases occurred in the rainy season. Also, much more cases of agricultural chemicals poisoning occurred in rainy season than the dry season. In terms of therapeutic effect, the treatment effect of paraquat was proven to be poor, for the death and unhealed cases got to 44.90% when discharge. The mortality rate of organophosphorus pesticides was also as high as 8.47%. Conclusion The incidence of acute poisoning in tropical areas of Yunnan Province is still high, especially caused by agricultural chemicals, wild mushroom, herbal medicines and poisonous plants. It is necessary to strengthen publicity and improve the emergency response system, in order to effectively reduce the occurrence and harm of acute poisoning accidents. 摘要：目的 探讨云南省热带地区急性中毒事件的发生规律和特点,为制定预防和控制措施提供依据。 方法 以 云南省的热带地区某三级综合医院为对象, 从该医院信息系统中调取 2013 年 1 月 1 日—2018 年 12 月 31 日出院的急性 中毒病例数据, 对中毒人群、中毒类型、中毒发生的季节及治疗效果等进行分析。 结果 该院 6 年期间共收治急性中毒 1 364 例, 各年龄段均有发生, 其中 20~<50 岁占总病例的 60.19%。导致中毒的物质中, 农药、化学药物、草药及植物性中 毒分别占总量的 48.39%、16.72% 和 11.80%, 其中百草枯、有机磷农药、野生菌、乌头碱、杀鼠剂分别为 244 例、189 例、96 例、90 例、89 例, 居前 5 位。导致中毒的热带毒性植物品类繁多。中毒病例发生在雨季的占总量的 57.26%, 其中野生菌 中毒几乎全部发生在雨季, 农药中毒雨季也显著高于旱季。治疗效果上, 百草枯治疗效果差, 出院时死亡和未愈病例 占 44.90%, 有机磷农药死亡率也高达 8.47%。 结论 云南省热带地区急性中毒发生率高, 其中农药类、野生菌、有毒植 物中毒问题突出。应加强宣传, 提高对急性中毒的关注, 完善应急处理体系,以期有效地降低急性中毒事件的发生及 危害。
{"title":"Analysis of 1 364 cases of acute poisoning in tropical areas of Yunnan","authors":"Li Tingting, Liu Haiyan, Wang Limei, Li Pinghua","doi":"10.13604/J.CNKI.46-1064/R.2020.11.06","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.11.06","url":null,"abstract":"Objective To investigate the occurrence and characteristics of acute poisoning events in the tropical regions of Yunnan Province, we provide evidence for prevention and control measures. Methods A three-level general hospital in typical tropical area of Yunnan Province was selected as the research object. All the cases of acute poisoning during 2013.01.01 to 2018.12.31 were retrieved from the information system to analyze the characteristics of population, the type of poisoning, the season of poisoning event and treatment effect. Results A total of 1 364 cases of acute poisoning were collected during the 6 years. Acute poisoning occurred in all age groups, and 20 to 50 age groups accounted for 60.19% of all cases. On poisoning substances, agricultural chemicals, drugs, herbal medicines and plant poisoning accounted for 48.39%, 16.72% and 11.80% respectively. Among them, paraquat, organophosphorus pesticide, wild mushroom, aconitine and rodenticide were 244, 189, 96, 90, 89 cases respectively, which took the top 5. A wide range of tropical poisonous plants that caused acute poisoning. Almost all the wild mushroom poisoning cases and 57.26% of all the poisoning cases occurred in the rainy season. Also, much more cases of agricultural chemicals poisoning occurred in rainy season than the dry season. In terms of therapeutic effect, the treatment effect of paraquat was proven to be poor, for the death and unhealed cases got to 44.90% when discharge. The mortality rate of organophosphorus pesticides was also as high as 8.47%. Conclusion The incidence of acute poisoning in tropical areas of Yunnan Province is still high, especially caused by agricultural chemicals, wild mushroom, herbal medicines and poisonous plants. It is necessary to strengthen publicity and improve the emergency response system, in order to effectively reduce the occurrence and harm of acute poisoning accidents. 摘要：目的 探讨云南省热带地区急性中毒事件的发生规律和特点,为制定预防和控制措施提供依据。 方法 以 云南省的热带地区某三级综合医院为对象, 从该医院信息系统中调取 2013 年 1 月 1 日—2018 年 12 月 31 日出院的急性 中毒病例数据, 对中毒人群、中毒类型、中毒发生的季节及治疗效果等进行分析。 结果 该院 6 年期间共收治急性中毒 1 364 例, 各年龄段均有发生, 其中 20~<50 岁占总病例的 60.19%。导致中毒的物质中, 农药、化学药物、草药及植物性中 毒分别占总量的 48.39%、16.72% 和 11.80%, 其中百草枯、有机磷农药、野生菌、乌头碱、杀鼠剂分别为 244 例、189 例、96 例、90 例、89 例, 居前 5 位。导致中毒的热带毒性植物品类繁多。中毒病例发生在雨季的占总量的 57.26%, 其中野生菌 中毒几乎全部发生在雨季, 农药中毒雨季也显著高于旱季。治疗效果上, 百草枯治疗效果差, 出院时死亡和未愈病例 占 44.90%, 有机磷农药死亡率也高达 8.47%。 结论 云南省热带地区急性中毒发生率高, 其中农药类、野生菌、有毒植 物中毒问题突出。应加强宣传, 提高对急性中毒的关注, 完善应急处理体系,以期有效地降低急性中毒事件的发生及 危害。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76934792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.13604/J.CNKI.46-1064/R.2020.11.01
Chen Jinyu, Wang Yujie, Liu Huanqing, Chen Dali
Objective Based on the (phosphoenolpyruvate carboxykinase, PEPCK) dominant epitopes gene of Leishmania infantum that has been analyzed and selected, we construct the corresponding recombinant prokaryotic expression vector to obtain the recombinant protein, construct the corresponding recombinant eukaryotic expression vector and verify the expression of eukaryotic vector in NIH3T3 cells, which laid the foundation for the subsequent vaccination and infection of animal experiment. Methods Based on the PEPCK dominant epitopes gene sequence, the recombinant prokaryotic and eukaryotic expression plasmid pET32a- PEPCK and pVAX1- EPEPCK were constructed by PCR reaction and enzyme reaction and were respectively transfected into E. coli and NIH3T3 cells of mice for expression. The expression of prokaryotic recombinant plasmid and the prokaryotic expressed protein purified by HisTrap affinity column were detected through SDS-PAGE and Western-Blot, and the expression of pVAX1-E PEPCK was verified by immunofluorescence. Results The correct sequencing results of the recombinant prokaryotic and eukaryotic vectors showed that the construction of recombinant vectors was successful. The positive results of 48.08 kD of recombinant protein were detected in SDS-PAGE and Western Blot, and immunofluorescence results of NIH3T3 cells transfected with eukaryotic recombinant vectors showed positive results. Conclusion We successfully constructed the recombinant prokaryotic and eukaryotic expression vectors: pET32a- PEPCK and pVAX1-E PEPCK , successfully got the recombinant proteins that followed by proteinic purification and confirmed the expression of pVAX1-E PEPCK in NIH3T3 cells, which is the basis for experiments where animals are vaccinated with a DNA prime-protein boost vaccines. 摘要：目的 基于前期分析并选取的婴儿利什曼原虫 PEPCK 的优势表位基因, 构建相应重组原核表达载体以获得 重组蛋白, 构建相应重组真核表达载体并验证真核载体在 NIH3T3 细胞中的表达, 为后续动物的免疫和感染实验备下 基础。 方法 根据 PEPCK 优势表位基因序列, 经 PCR 反应及酶切连接构建重组原核与真核表达质粒 pET32a- PEPCK 和 pVAX1-E PEPCK 并分别转染至 E. coli 和 NIH3T3 细胞中进行表达。采用 SDS-PAGE 电泳和 Western Blot 鉴定原核重 组质粒在大肠杆菌中的表达和原核表达蛋白被镍柱纯化后的情况, 采用免疫荧光实验验证真核重组质粒在细胞中的 表达。 结果 重组原核与真核载体的正确测序结果表明重组载体构建成功。SDS-PAGE 电泳和 Western Blot 的结果 显示, 大肠杆菌中表达的原核重组蛋白以及纯化后的蛋白在 48.08 kD 处存在条带, 转染了真核重组载体的 NIH3T3 细胞 的免疫荧光结果呈阳性。 结论 成功构建了 PEPCK 优势表位基因的重组原核和真核表达载体: pET32a- PEPCK 和 pVAX1-E PEPCK , 成功表达相应的重组蛋白并纯化并验证了 pVAX1-E PEPCK 在 NIH3T3 细胞中的表达, 为后续 DNA 疫苗初次免疫和蛋白疫苗加强免疫的动物实验备下基础。
{"title":"Molecular cloning and expression of hosphoenolpyruvate carboxykinase dominant epitope in Leishmania infantum","authors":"Chen Jinyu, Wang Yujie, Liu Huanqing, Chen Dali","doi":"10.13604/J.CNKI.46-1064/R.2020.11.01","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.11.01","url":null,"abstract":"Objective Based on the (phosphoenolpyruvate carboxykinase, PEPCK) dominant epitopes gene of Leishmania infantum that has been analyzed and selected, we construct the corresponding recombinant prokaryotic expression vector to obtain the recombinant protein, construct the corresponding recombinant eukaryotic expression vector and verify the expression of eukaryotic vector in NIH3T3 cells, which laid the foundation for the subsequent vaccination and infection of animal experiment. Methods Based on the PEPCK dominant epitopes gene sequence, the recombinant prokaryotic and eukaryotic expression plasmid pET32a- PEPCK and pVAX1- EPEPCK were constructed by PCR reaction and enzyme reaction and were respectively transfected into E. coli and NIH3T3 cells of mice for expression. The expression of prokaryotic recombinant plasmid and the prokaryotic expressed protein purified by HisTrap affinity column were detected through SDS-PAGE and Western-Blot, and the expression of pVAX1-E PEPCK was verified by immunofluorescence. Results The correct sequencing results of the recombinant prokaryotic and eukaryotic vectors showed that the construction of recombinant vectors was successful. The positive results of 48.08 kD of recombinant protein were detected in SDS-PAGE and Western Blot, and immunofluorescence results of NIH3T3 cells transfected with eukaryotic recombinant vectors showed positive results. Conclusion We successfully constructed the recombinant prokaryotic and eukaryotic expression vectors: pET32a- PEPCK and pVAX1-E PEPCK , successfully got the recombinant proteins that followed by proteinic purification and confirmed the expression of pVAX1-E PEPCK in NIH3T3 cells, which is the basis for experiments where animals are vaccinated with a DNA prime-protein boost vaccines. 摘要：目的 基于前期分析并选取的婴儿利什曼原虫 PEPCK 的优势表位基因, 构建相应重组原核表达载体以获得 重组蛋白, 构建相应重组真核表达载体并验证真核载体在 NIH3T3 细胞中的表达, 为后续动物的免疫和感染实验备下 基础。 方法 根据 PEPCK 优势表位基因序列, 经 PCR 反应及酶切连接构建重组原核与真核表达质粒 pET32a- PEPCK 和 pVAX1-E PEPCK 并分别转染至 E. coli 和 NIH3T3 细胞中进行表达。采用 SDS-PAGE 电泳和 Western Blot 鉴定原核重 组质粒在大肠杆菌中的表达和原核表达蛋白被镍柱纯化后的情况, 采用免疫荧光实验验证真核重组质粒在细胞中的 表达。 结果 重组原核与真核载体的正确测序结果表明重组载体构建成功。SDS-PAGE 电泳和 Western Blot 的结果 显示, 大肠杆菌中表达的原核重组蛋白以及纯化后的蛋白在 48.08 kD 处存在条带, 转染了真核重组载体的 NIH3T3 细胞 的免疫荧光结果呈阳性。 结论 成功构建了 PEPCK 优势表位基因的重组原核和真核表达载体: pET32a- PEPCK 和 pVAX1-E PEPCK , 成功表达相应的重组蛋白并纯化并验证了 pVAX1-E PEPCK 在 NIH3T3 细胞中的表达, 为后续 DNA 疫苗初次免疫和蛋白疫苗加强免疫的动物实验备下基础。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73766171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.13604/J.CNKI.46-1064/R.2020.11.09
Ling Feng, Qu Zhi-qiang, Shi Jian, Luo Mi-fang
Objective Summary and analysis the epidemiological characteristics and the emergency handling of Dengue Fever disposal in Jiangnan district of Nanningcity in 2019, we provide scientific evidence for effective prevention and control measures in future. Methods Epidemiologic method was used to analyze the occurrence of dengue fever in Jiangnan district in 2019, and we evaluate the results on Dengue Fever disposal. Results The situation of dengue fever of Jiangnan district in 2019 was severe.There were 370 cases of dengue fever reported,including 4 imported cases and 366 local cases. Dengue Fever infection was mainly in unemployment in the home and retired; the male to female ratio of the cases was 1∶1.12, the age of the cases ranged from 1year to 92 years. The spatial distribution of the onset was highly clustered, Fujian Yuan street accounted for 87.70% of the local cases in the city. The qualification rate of Breteau index (BI) and bed net trap index in early emergency monitoring of Aedes mosquitoes was low, were 72.17% and 62.61% respectively. According to the characteristics of the epidemic, specific prevention and control strategies should be formulated to deal with the epidemic in a precise manner, effectively reduce the peak value and quickly control the spread of the epidemic. Conclusion The dengue epidemic was caused by imported cases, which led to an outbreak at the community level. The epidemic showed a trend of multiple outbreaks and spread. We need to do a good job in investigating the epidemic, early warning and prediction, accurate identification of epidemiological characteristics and early implementation of dengue emergency response. Accurate prevention and control, breeding site treatment, health education and case management are the key measures for emergency response. 摘要：目的 分析总结南宁市江南区 2019 年登革热流行病学特征和疫情应急处置的工作情况, 为今后有效地防制 登革热提供对策、参考和技术支持。 方法 收集南宁市江南区 2019 年登革热疫情相关数据, 评价本次应急处置的工作 成效。 结果 2019 年南宁市江南区登革热疫情严峻, 共报告登革热病例 370 例, 其中输入病例 4 例, 本地病例 366 例; 感 染人数以家务待业和离退休者居多; 男女性别比为 1∶1.12; 发病年龄最小 1 岁, 最大 92 岁; 发病的空间分布呈现高度聚 集, 福建园街道占本城区本地病例的 87.70%。早期伊蚊应急监测布雷图指数和账诱指数合格率偏低, 分别为 72.17% 和 62.61%。针对本次疫情特性, 制定有针对性的防控策略, 做好精准疫情应急处置, 有效压低峰值, 迅速控制了疫情的 扩散和蔓延。 结论 本次疫情是由输入性病例导致本地病例社区水平暴发, 疫情呈现多点暴发及扩散蔓延态势。需 做好疫情研判、预警预测, 准确分析流行病学特征, 尽早实施登革热应急处置, 精准防控、孳生地处理、健康宣教和病例 管理是应急处置的关键措施。
{"title":"Analysis on the epidemiological characteristics and effect about emergency handling of dengue fever in Jiangnan district of Nanning, 2019","authors":"Ling Feng, Qu Zhi-qiang, Shi Jian, Luo Mi-fang","doi":"10.13604/J.CNKI.46-1064/R.2020.11.09","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.11.09","url":null,"abstract":"Objective Summary and analysis the epidemiological characteristics and the emergency handling of Dengue Fever disposal in Jiangnan district of Nanningcity in 2019, we provide scientific evidence for effective prevention and control measures in future. Methods Epidemiologic method was used to analyze the occurrence of dengue fever in Jiangnan district in 2019, and we evaluate the results on Dengue Fever disposal. Results The situation of dengue fever of Jiangnan district in 2019 was severe.There were 370 cases of dengue fever reported,including 4 imported cases and 366 local cases. Dengue Fever infection was mainly in unemployment in the home and retired; the male to female ratio of the cases was 1∶1.12, the age of the cases ranged from 1year to 92 years. The spatial distribution of the onset was highly clustered, Fujian Yuan street accounted for 87.70% of the local cases in the city. The qualification rate of Breteau index (BI) and bed net trap index in early emergency monitoring of Aedes mosquitoes was low, were 72.17% and 62.61% respectively. According to the characteristics of the epidemic, specific prevention and control strategies should be formulated to deal with the epidemic in a precise manner, effectively reduce the peak value and quickly control the spread of the epidemic. Conclusion The dengue epidemic was caused by imported cases, which led to an outbreak at the community level. The epidemic showed a trend of multiple outbreaks and spread. We need to do a good job in investigating the epidemic, early warning and prediction, accurate identification of epidemiological characteristics and early implementation of dengue emergency response. Accurate prevention and control, breeding site treatment, health education and case management are the key measures for emergency response. 摘要：目的 分析总结南宁市江南区 2019 年登革热流行病学特征和疫情应急处置的工作情况, 为今后有效地防制 登革热提供对策、参考和技术支持。 方法 收集南宁市江南区 2019 年登革热疫情相关数据, 评价本次应急处置的工作 成效。 结果 2019 年南宁市江南区登革热疫情严峻, 共报告登革热病例 370 例, 其中输入病例 4 例, 本地病例 366 例; 感 染人数以家务待业和离退休者居多; 男女性别比为 1∶1.12; 发病年龄最小 1 岁, 最大 92 岁; 发病的空间分布呈现高度聚 集, 福建园街道占本城区本地病例的 87.70%。早期伊蚊应急监测布雷图指数和账诱指数合格率偏低, 分别为 72.17% 和 62.61%。针对本次疫情特性, 制定有针对性的防控策略, 做好精准疫情应急处置, 有效压低峰值, 迅速控制了疫情的 扩散和蔓延。 结论 本次疫情是由输入性病例导致本地病例社区水平暴发, 疫情呈现多点暴发及扩散蔓延态势。需 做好疫情研判、预警预测, 准确分析流行病学特征, 尽早实施登革热应急处置, 精准防控、孳生地处理、健康宣教和病例 管理是应急处置的关键措施。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"387 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84989055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.13604/J.CNKI.46-1064/R.2020.11.12
Xu Wei, Zhang Xiuzhi, Zhang Aijie, W. Yunfang
Objective To supply evidence with prevention and control of drug-resistant tuberculosis in school, through investigation and analysis of the epidemic cluster of drug-resistant tuberculosis in a school of Chaoyang District, Beijing. Methods The cases were investigated of epidemiology, PPD, chest X-ray and CT screening were carried out in close contacts of patients. Results From October 2018 to June 2019, 36 cases of pulmonary tuberculosis occurred in the school, with an incidence of 4.5%. 5 were multi-drug resistant, and 3 were rifampicin resistant. 36 cases were mainly distributed in four classes, 27 cases in class 15a, 4 in class 15b, 2 in class 15c, and 3 in class 17d. The incidence rate of each class was 56.3%, 8.3%, 5.7% and 7.1% respectively. The difference was statistically significant ( P <0.01). 6 of the 8 drug-resistant cases were students of class 15a, accounting for 75.0% of the total cases. 23 cases of tuberculosis were diagnosed by CT screening in 68 close contacts, the detection rate were 33.8%. Conclusions It is the first case to deal with the cluster epidemic of drug- resistant tuberculosis transmission in schools. The main reason for the outbreak was that the first case was not treated in time and the source of infection existed for a long time. Close screening is very important for the timely detection of new cases in school cluster epidemic management. 摘要：目的 对北京市朝阳区某学校一起耐药肺结核疫情进行分析, 为今后耐药结核病疫情的处置提供参考。 方法 对病例进行流行病学调查, 对病例密切接触者采用 PPD 试验、X 线胸片和 CT 相结合的方式筛查。 结果 2018 年 10 月—2019 年 6 月, 该校共发生 36 例肺结核病例, 发病率为 4.5%。其中 5 例耐多药, 3 例耐利福平。36 例病例分布在 四个班, 15a 班 27 例、15b 班 4 例、15c 班 2 例、17d 班 3 例。各班发病率分别为 56.3%、8.3%、5.7%、7.1%, 差异有统计学意 义 ( P <0.01)。8 例耐药病例中 6 例为 15a 班学生, 占耐药病例总数的 75.0%。经 CT 筛查 68 名密切接触者中确诊 23 例肺 结核患者, 检出率 33.8%。 结论 该起学校聚集性疫情为全国首起耐药肺结核聚集性疫情, 首发病例未及时就医, 传染 源隐匿存在时间长, 是导致该起疫情发生的主要原因。疫情处置中开展密切接触者筛查对于及时发现新病例非常 重要。
{"title":"Investigation of a drug-resistant tuberculosis cluster in a school","authors":"Xu Wei, Zhang Xiuzhi, Zhang Aijie, W. Yunfang","doi":"10.13604/J.CNKI.46-1064/R.2020.11.12","DOIUrl":"https://doi.org/10.13604/J.CNKI.46-1064/R.2020.11.12","url":null,"abstract":"Objective To supply evidence with prevention and control of drug-resistant tuberculosis in school, through investigation and analysis of the epidemic cluster of drug-resistant tuberculosis in a school of Chaoyang District, Beijing. Methods The cases were investigated of epidemiology, PPD, chest X-ray and CT screening were carried out in close contacts of patients. Results From October 2018 to June 2019, 36 cases of pulmonary tuberculosis occurred in the school, with an incidence of 4.5%. 5 were multi-drug resistant, and 3 were rifampicin resistant. 36 cases were mainly distributed in four classes, 27 cases in class 15a, 4 in class 15b, 2 in class 15c, and 3 in class 17d. The incidence rate of each class was 56.3%, 8.3%, 5.7% and 7.1% respectively. The difference was statistically significant ( P <0.01). 6 of the 8 drug-resistant cases were students of class 15a, accounting for 75.0% of the total cases. 23 cases of tuberculosis were diagnosed by CT screening in 68 close contacts, the detection rate were 33.8%. Conclusions It is the first case to deal with the cluster epidemic of drug- resistant tuberculosis transmission in schools. The main reason for the outbreak was that the first case was not treated in time and the source of infection existed for a long time. Close screening is very important for the timely detection of new cases in school cluster epidemic management. 摘要：目的 对北京市朝阳区某学校一起耐药肺结核疫情进行分析, 为今后耐药结核病疫情的处置提供参考。 方法 对病例进行流行病学调查, 对病例密切接触者采用 PPD 试验、X 线胸片和 CT 相结合的方式筛查。 结果 2018 年 10 月—2019 年 6 月, 该校共发生 36 例肺结核病例, 发病率为 4.5%。其中 5 例耐多药, 3 例耐利福平。36 例病例分布在 四个班, 15a 班 27 例、15b 班 4 例、15c 班 2 例、17d 班 3 例。各班发病率分别为 56.3%、8.3%、5.7%、7.1%, 差异有统计学意 义 ( P <0.01)。8 例耐药病例中 6 例为 15a 班学生, 占耐药病例总数的 75.0%。经 CT 筛查 68 名密切接触者中确诊 23 例肺 结核患者, 检出率 33.8%。 结论 该起学校聚集性疫情为全国首起耐药肺结核聚集性疫情, 首发病例未及时就医, 传染 源隐匿存在时间长, 是导致该起疫情发生的主要原因。疫情处置中开展密切接触者筛查对于及时发现新病例非常 重要。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"320 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82911105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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