Pub Date : 2025-06-30DOI: 10.1016/j.jfutfo.2024.05.016
Yujing Li , Erjing Zhang , Hejie Li , Yan Zhang , Jing Zhang , Chunsheng Li , Jingjing Liu
A rapid enzyme-linked immunosorbent assay (ELISA) system for the detection of fipronil was studied. Artificial antigens were synthesized through the glutaraldehyde method, and monoclonal antibodies were obtained via immunization and fusion techniques. The concentration of the coated antigen and antibody and the reaction time were determined, and the recovery rate was determined by an ELISA detection system. The cross-reaction rate of the antibody against fipronil metabolites was lower than 21.46%. The response time of the selected ELISA system was 30 min shorter than that of the competitive ELISA system. The IC20 to IC80 of the standard curve ranged from 0.139 to 1.433 μg/L. The recovery rate of fipronil spiked in eggs was 76.7%–93.7%. This study provides support for the rapid ELISA of fipronil.
{"title":"A one-step enzyme-linked immunosorbent assay for fipronil detection","authors":"Yujing Li , Erjing Zhang , Hejie Li , Yan Zhang , Jing Zhang , Chunsheng Li , Jingjing Liu","doi":"10.1016/j.jfutfo.2024.05.016","DOIUrl":"10.1016/j.jfutfo.2024.05.016","url":null,"abstract":"<div><div>A rapid enzyme-linked immunosorbent assay (ELISA) system for the detection of fipronil was studied. Artificial antigens were synthesized through the glutaraldehyde method, and monoclonal antibodies were obtained via immunization and fusion techniques. The concentration of the coated antigen and antibody and the reaction time were determined, and the recovery rate was determined by an ELISA detection system. The cross-reaction rate of the antibody against fipronil metabolites was lower than 21.46%. The response time of the selected ELISA system was 30 min shorter than that of the competitive ELISA system. The IC<sub>20</sub> to IC<sub>80</sub> of the standard curve ranged from 0.139 to 1.433 μg/L. The recovery rate of fipronil spiked in eggs was 76.7%–93.7%. This study provides support for the rapid ELISA of fipronil.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 2","pages":"Pages 301-306"},"PeriodicalIF":7.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-30DOI: 10.1016/j.jfutfo.2024.05.014
Shengyan Dong , Jiaxuan Chen , Shilei Li , Yingying Li , Xiaojuan Liang , Yisen Liu , Mingyue Zhang , Feng Yang , Rongjuan Feng , Jun Liang
This work aims to study the cultural conditions of pigeon muscle satellite cells and illustrate the nutritional composition of the pigeon-derived cultivated meat. Methods for cell disaggregation and purification were optimized, high-purity pigeon skeletal muscle satellite cells were successfully isolated. Meanwhile, the cultivation conditions for cell proliferation and multi-directional differentiation were optimized. Results demonstrated that pigeon muscle satellite cells exhibited rapid proliferation and spontaneous differentiation at 41 °C, McCoy's 5A was significantly superior to other cultural mediums for cell growth, DMEM with high glucose promote myogenic differentiation of pigeon skeletal muscle satellite cells and chicken serum can play important role in inducing the pigeon muscle satellite cells into adipocytes. Additionally, increasing the concentration of serum in medium has a positive promoting effect on the respective effects. Nutritional composition and biomarkers of the pigeon-derived cultivated meat was first illustrated in this work by proteomics and lipidomics, respectively. Proteomics revealed that 140 proteins were identified as major aberrant proteins and 648 proteins were identified from the after myogenic differentiation samples, including 12 collagens, 15 myosins, 7 actins, etc. Lipidomics revealed that 73 lipids were identified as major aberrant lipids and 297 lipids were identified from the after lipidogenic differentiation, including 102 triglycerides, 52 diacylglycerols, 59 phosphatidyl cholines, etc.
{"title":"Cultural optimization of pigeon muscle satellite cells and nutritional evaluation of the pigeon-derived cultivated meat","authors":"Shengyan Dong , Jiaxuan Chen , Shilei Li , Yingying Li , Xiaojuan Liang , Yisen Liu , Mingyue Zhang , Feng Yang , Rongjuan Feng , Jun Liang","doi":"10.1016/j.jfutfo.2024.05.014","DOIUrl":"10.1016/j.jfutfo.2024.05.014","url":null,"abstract":"<div><div>This work aims to study the cultural conditions of pigeon muscle satellite cells and illustrate the nutritional composition of the pigeon-derived cultivated meat. Methods for cell disaggregation and purification were optimized, high-purity pigeon skeletal muscle satellite cells were successfully isolated. Meanwhile, the cultivation conditions for cell proliferation and multi-directional differentiation were optimized. Results demonstrated that pigeon muscle satellite cells exhibited rapid proliferation and spontaneous differentiation at 41 °C, McCoy's 5A was significantly superior to other cultural mediums for cell growth, DMEM with high glucose promote myogenic differentiation of pigeon skeletal muscle satellite cells and chicken serum can play important role in inducing the pigeon muscle satellite cells into adipocytes. Additionally, increasing the concentration of serum in medium has a positive promoting effect on the respective effects. Nutritional composition and biomarkers of the pigeon-derived cultivated meat was first illustrated in this work by proteomics and lipidomics, respectively. Proteomics revealed that 140 proteins were identified as major aberrant proteins and 648 proteins were identified from the after myogenic differentiation samples, including 12 collagens, 15 myosins, 7 actins, etc. Lipidomics revealed that 73 lipids were identified as major aberrant lipids and 297 lipids were identified from the after lipidogenic differentiation, including 102 triglycerides, 52 diacylglycerols, 59 phosphatidyl cholines, etc.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 2","pages":"Pages 251-265"},"PeriodicalIF":7.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-30DOI: 10.1016/j.jfutfo.2025.06.006
Shaimaa H Negm , Walaa A.M. Eid , Hany Gaber El-Masry
Nonalcoholic fatty liver disease (NAFLD) is a liver disorder causes people who drink little or no alcohol to accumulate extra fat in their livers. It's getting prevalent in those who are obese. This study was aimed to investigate effects of the consumption of bread fortified with lupin seeds (LS) and psyllium husk (PSH) on NAFLD. Forty-two male rats were divided into 6 groups of 7 animals including 2 groups served as negative controls were given a basal diet and another a basal diet supplemented with unfortified bread. While, the rest of rats (n = 28) were administration with 25% fructose dissolved in water for 8 weeks to induce NAFLD. Then reclassify into 4 subgroups, only one fed on basal diet containing un-fortified bread served as positive control group and three subgroups fed on bread fortified with 10% of (LS or PSH or their mixture) powder/kg diet respectively, for 28 days. The results observed an improvement in the nutritious qualities of bread with addition of (LS or PSH), especially in terms of proteins, fiber, total phenolic compounds (TPC) and antioxidant (AA). Bread with 10% LS showed highest (TPC and AA), which recorded (2.96 mg GAE/g and 78.15%), while the flavonoids showed highest value in 10% (LS + PSHS) bread. Biological results all treated rat groups revealed improve in body weight, lipid profile and glycemic indices. Similarly, bread fortified with LS and PSH reduced liver (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase) and kidney function (uric acid, urea, creatinine). Interestingly, LS and PSH effectively prevented the increase thiobarbituric acid reactive substances value, but increased levels of reduced glutathione, superoxide dismutase and catalase. Also effectively protected liver from histological changes. Conclusion, LS or PSH is excellent resources of natural antioxidants that might be added to functional foods an important agent for the protection from NAFLD.
{"title":"The Effects of Bread Fortification with Lupin seeds and Psyllium Husk on Nonalcoholic Fatty Liver Disease in Rats","authors":"Shaimaa H Negm , Walaa A.M. Eid , Hany Gaber El-Masry","doi":"10.1016/j.jfutfo.2025.06.006","DOIUrl":"10.1016/j.jfutfo.2025.06.006","url":null,"abstract":"<div><div>Nonalcoholic fatty liver disease (NAFLD) is a liver disorder causes people who drink little or no alcohol to accumulate extra fat in their livers. It's getting prevalent in those who are obese. This study was aimed to investigate effects of the consumption of bread fortified with lupin seeds (LS) and psyllium husk (PSH) on NAFLD. Forty-two male rats were divided into 6 groups of 7 animals including 2 groups served as negative controls were given a basal diet and another a basal diet supplemented with unfortified bread. While, the rest of rats (<em>n</em> = 28) were administration with 25% fructose dissolved in water for 8 weeks to induce NAFLD. Then reclassify into 4 subgroups, only one fed on basal diet containing un-fortified bread served as positive control group and three subgroups fed on bread fortified with 10% of (LS or PSH or their mixture) powder/kg diet respectively, for 28 days. The results observed an improvement in the nutritious qualities of bread with addition of (LS or PSH), especially in terms of proteins, fiber, total phenolic compounds (TPC) and antioxidant (AA). Bread with 10% LS showed highest (TPC and AA), which recorded (2.96 mg GAE/g and 78.15%), while the flavonoids showed highest value in 10% (LS + PSHS) bread. Biological results all treated rat groups revealed improve in body weight, lipid profile and glycemic indices. Similarly, bread fortified with LS and PSH reduced liver (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase) and kidney function (uric acid, urea, creatinine). Interestingly, LS and PSH effectively prevented the increase thiobarbituric acid reactive substances value, but increased levels of reduced glutathione, superoxide dismutase and catalase. Also effectively protected liver from histological changes. Conclusion, LS or PSH is excellent resources of natural antioxidants that might be added to functional foods an important agent for the protection from NAFLD.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 5","pages":"Pages 850-861"},"PeriodicalIF":7.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145718722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-30DOI: 10.1016/j.jfutfo.2024.09.005
Guihong Yao , Yun Ling , Tong Liu , Shige Xing , Meiyi Yao , Wei Guo , Feng Zhang
Herein, a green and facile enzyme immobilization method was developed by immobilizing enzymes on the bamboo charcoal (BC) through gold-mediated adsorption under mild conditions (no organic solvents were required). Lipase PS was used as the model enzyme, and the obtained immobilized lipase (PS@BC-Au) exhibited a high immobilization efficiency (93.9%) and enzyme loading capacity (234.78 µg/mg). The half-life of PS@BC-Au was found to be 362.8 min, which is approximately 1.3 and 2 times longer than that of the free enzyme (185.3 min) and the immobilized enzyme without gold (PS@BC, 275.0 min). Furthermore, PS@BC-Au and PS@BC exhibited up to 63.9% and 47.1% of residual activity after 10 cycles, respectively. Interestingly, the gold nanoparticles in the immobilized system can improve the catalytic efficiency. Consequently, BC, with its porous structure, good stability, large surface area, low cost, and environmental-friendly properties, can serve as a promising support for enzyme immobilized application.
{"title":"Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization","authors":"Guihong Yao , Yun Ling , Tong Liu , Shige Xing , Meiyi Yao , Wei Guo , Feng Zhang","doi":"10.1016/j.jfutfo.2024.09.005","DOIUrl":"10.1016/j.jfutfo.2024.09.005","url":null,"abstract":"<div><div>Herein, a green and facile enzyme immobilization method was developed by immobilizing enzymes on the bamboo charcoal (BC) through gold-mediated adsorption under mild conditions (no organic solvents were required). Lipase PS was used as the model enzyme, and the obtained immobilized lipase (PS@BC-Au) exhibited a high immobilization efficiency (93.9%) and enzyme loading capacity (234.78 µg/mg). The half-life of PS@BC-Au was found to be 362.8 min, which is approximately 1.3 and 2 times longer than that of the free enzyme (185.3 min) and the immobilized enzyme without gold (PS@BC, 275.0 min). Furthermore, PS@BC-Au and PS@BC exhibited up to 63.9% and 47.1% of residual activity after 10 cycles, respectively. Interestingly, the gold nanoparticles in the immobilized system can improve the catalytic efficiency. Consequently, BC, with its porous structure, good stability, large surface area, low cost, and environmental-friendly properties, can serve as a promising support for enzyme immobilized application.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 2","pages":"Pages 244-250"},"PeriodicalIF":7.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The demand for traditional dairy products in the form of ready to eat with extended shelf life has increased steadily, and to prepare them, minimum moisture has to be maintained by the application of different drying technologies. These technologies have some limitations and these can be overcome by application of foam-mat drying. This method has clear benefits, as it is capable of maintaining nutritional and sensory qualities, providing improved energy efficiency, and reducing costs. This paper covers the applications of foam mat drying in various dairy products, including yogurt, cheese, and whey powder. The challenges linked to foam stability and operational complexity need to be addressed for large scale implementation of foam mat drying.
{"title":"Applications of foam-mat drying for milk and milk products: an innovative preservation technique","authors":"Dnyaneshwar Devrao Patange , Shital Appasaheb Patil , Snehal Pranav Khandekar","doi":"10.1016/j.jfutfo.2024.05.013","DOIUrl":"10.1016/j.jfutfo.2024.05.013","url":null,"abstract":"<div><div>The demand for traditional dairy products in the form of ready to eat with extended shelf life has increased steadily, and to prepare them, minimum moisture has to be maintained by the application of different drying technologies. These technologies have some limitations and these can be overcome by application of foam-mat drying. This method has clear benefits, as it is capable of maintaining nutritional and sensory qualities, providing improved energy efficiency, and reducing costs. This paper covers the applications of foam mat drying in various dairy products, including yogurt, cheese, and whey powder. The challenges linked to foam stability and operational complexity need to be addressed for large scale implementation of foam mat drying.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 2","pages":"Pages 184-194"},"PeriodicalIF":7.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-28DOI: 10.1016/j.jfutfo.2024.12.005
Kaiyue Su , Xinli Peng , Yu Su , Geoffrey I.N. Waterhouse , Xuguang Qiao , Zhixiang Xu
In this work, a magnetic molecularly imprinted polymer with good adsorption capacity and a 2-methylimidazole (MI)-Cu-guanosine 5′-monophosphoric acid (GMP) nanozyme with high catalysis activity were first synthesized. Then, using the imprinted polymer as biomimetic antibody and the MI-Cu-GMP nanozyme as label, a novel direct competitive biomimetic enzyme-linked immunoassay (BELISA) method was established for the sensitive detection of acrylamide in foods. Under optimal conditions, the limit of detection (LOD, IC15) and sensitivity (IC50) of the BELISA method towards acrylamide were 0.02 and 4.52 mg/L, respectively. The BELISA method was successfully applied for acrylamide detection in spike potato crisp samples, with recoveries ranging from 87.50% to 106.67% being obtained. Further, the levels of acrylamide in coffee, fried dough twists and biscuit samples were determined by the BELISA and high performance liquid chromatography, with no significant difference found between the results of the two methods. This work thus introduces a novel, fast and accurate method for acrylamide detection in foods.
{"title":"A sensitive biomimetic immunoassay method for the detection of acrylamide in foods based on a magnetic molecularly imprinted biomimetic antibody and nanozyme label","authors":"Kaiyue Su , Xinli Peng , Yu Su , Geoffrey I.N. Waterhouse , Xuguang Qiao , Zhixiang Xu","doi":"10.1016/j.jfutfo.2024.12.005","DOIUrl":"10.1016/j.jfutfo.2024.12.005","url":null,"abstract":"<div><div>In this work, a magnetic molecularly imprinted polymer with good adsorption capacity and a 2-methylimidazole (MI)-Cu-guanosine 5′-monophosphoric acid (GMP) nanozyme with high catalysis activity were first synthesized. Then, using the imprinted polymer as biomimetic antibody and the MI-Cu-GMP nanozyme as label, a novel direct competitive biomimetic enzyme-linked immunoassay (BELISA) method was established for the sensitive detection of acrylamide in foods. Under optimal conditions, the limit of detection (LOD, IC<sub>15</sub>) and sensitivity (IC<sub>50</sub>) of the BELISA method towards acrylamide were 0.02 and 4.52 mg/L, respectively. The BELISA method was successfully applied for acrylamide detection in spike potato crisp samples, with recoveries ranging from 87.50% to 106.67% being obtained. Further, the levels of acrylamide in coffee, fried dough twists and biscuit samples were determined by the BELISA and high performance liquid chromatography, with no significant difference found between the results of the two methods. This work thus introduces a novel, fast and accurate method for acrylamide detection in foods.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 5","pages":"Pages 796-802"},"PeriodicalIF":7.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145718717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-28DOI: 10.1016/j.jfutfo.2024.11.014
Xiaoyu Chen , Hongyuan Zhao , Fanqiang Meng , Juan Shen , Chao Tang , Zhaoxin Lu , Yingjian Lu
Type 2 diabetes mellitus (T2DM) is accompanied by insulin resistance in liver and peripheral tissue. Bacillus amyloliquefaciens fmb50 produces lipopeptide surfactin, which has a wide range of biological activities. However, the effects of surfactin on insulin resistance in any mouse model have not been reported. Our previous research demonstrated that dietary supplementation with surfactin can alleviate high fat diet (HFD)/ streptozotocin (STZ)-induced T2DM in mice by regulating intestinal microbiota. This study aims to explore the detailed mechanism of the effect of surfactin on liver and peripheral tissue. The results indicated that surfactin inhibited hepatic gluconeogenesis and promoted hepatic glycogen synthesis by activating the adenosine monophosphate-activated protein kinase and phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt) signalling pathways. Similarly, surfactin also significantly increased muscle glycogen synthesis by activating the PI3K/Akt signalling pathway. The glucose transporter 4 levels of skeletal muscle and adipose tissue, which are insulin-mediated vital organs, was noticeably increased after surfactin treatment. In addition, our results indicate that decreased hepatic fat accumulation and suppressed inflammation and oxidative stress in liver, skeletal muscle and adipose tissue also play critical roles in ameliorating insulin resistance. These findings demonstrate that surfactin not only ameliorates liver dysfunction but also enhances insulin sensitivity in HFD/STZ-induced T2DM mice. Finally, using fasting blood glucose, oral glucose tolerance test and insulin tolerance test, we show that early surfactin intervention can mitigate T2DM in mice. The study results warrant future functional food studies which will provide insight into diet-host interactions.
{"title":"Surfactin alleviates insulin resistance by modulating the AMPK/PI3K/Akt signalling pathway and inactivating inflammatory pathways","authors":"Xiaoyu Chen , Hongyuan Zhao , Fanqiang Meng , Juan Shen , Chao Tang , Zhaoxin Lu , Yingjian Lu","doi":"10.1016/j.jfutfo.2024.11.014","DOIUrl":"10.1016/j.jfutfo.2024.11.014","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) is accompanied by insulin resistance in liver and peripheral tissue. <em>Bacillus amyloliquefaciens</em> fmb50 produces lipopeptide surfactin, which has a wide range of biological activities. However, the effects of surfactin on insulin resistance in any mouse model have not been reported. Our previous research demonstrated that dietary supplementation with surfactin can alleviate high fat diet (HFD)/ streptozotocin (STZ)-induced T2DM in mice by regulating intestinal microbiota. This study aims to explore the detailed mechanism of the effect of surfactin on liver and peripheral tissue. The results indicated that surfactin inhibited hepatic gluconeogenesis and promoted hepatic glycogen synthesis by activating the adenosine monophosphate-activated protein kinase and phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt) signalling pathways. Similarly, surfactin also significantly increased muscle glycogen synthesis by activating the PI3K/Akt signalling pathway. The glucose transporter 4 levels of skeletal muscle and adipose tissue, which are insulin-mediated vital organs, was noticeably increased after surfactin treatment. In addition, our results indicate that decreased hepatic fat accumulation and suppressed inflammation and oxidative stress in liver, skeletal muscle and adipose tissue also play critical roles in ameliorating insulin resistance. These findings demonstrate that surfactin not only ameliorates liver dysfunction but also enhances insulin sensitivity in HFD/STZ-induced T2DM mice. Finally, using fasting blood glucose, oral glucose tolerance test and insulin tolerance test, we show that early surfactin intervention can mitigate T2DM in mice. The study results warrant future functional food studies which will provide insight into diet-host interactions.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 5","pages":"Pages 881-893"},"PeriodicalIF":7.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145718725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-28DOI: 10.1016/j.jfutfo.2025.01.005
Li Zhen , Qian Wang , Huimin Cui , Yifen Wang , Jianxin Cao , Yaping Liu , Hesham R El-Seedi , Guiguang Cheng
Goji (Lycium barbarum L.) leaves are widely processed as a healthy tea (GT) in China for their multiple biological activities. In this study, different polar segments from GT ethanolic extract were obtained using the liquid-liquid fractionation method. n-Butanol fraction (GN) had the highest antioxidant activity while ethyl acetate fraction (GE) showed the highest inhibitory effect on the α-glucosidase enzyme. The anti-diabetic effect of GE was further investigated in type 2 diabetic mellitus (T2DM) mice. The oral administration of GE (300 or 600 mg/kg) reduced fasting blood glucose levels, improved glucose tolerance ability, and ameliorated insulin resistance in T2DM mice. GE intervention could enhance the cholesterol transportation in the serum in coincidence with decreased total cholesterol, triglyceride levels, and non-esterified fatty acid. Transcriptomic, immunofluorescence and western blotting analysis revealed that GE significantly up-regulated insulin receptor substrate 1, phosphatidylinositol-3-kinase, protein kinase B, glycogen synthesis, and glucose transporter 4. AMP-activated protein kinases-mediate gluconeogenesis and glycogenolysis, such as phosphoenolpyruvate carboxy kinase1 and glucose-6-phosphatase in the liver and kidney tissues of GE groups were decreased. Thus, GE alleviated the liver, kidney, and pancreatic tissue damage. The phytoconstituents of GE were characterized, and rutin, neochlorogenic acid, and its acylated derivatives have hypoglycemic effects. Furthermore, these findings suggested that GT might be used as a promising hypoglycemic candidate.
{"title":"Hypoglycemic effects of goji tea in streptozotocin-induced diabetic mice via IRS1/PI3K/Akt/AMPK pathway","authors":"Li Zhen , Qian Wang , Huimin Cui , Yifen Wang , Jianxin Cao , Yaping Liu , Hesham R El-Seedi , Guiguang Cheng","doi":"10.1016/j.jfutfo.2025.01.005","DOIUrl":"10.1016/j.jfutfo.2025.01.005","url":null,"abstract":"<div><div>Goji (<em>Lycium barbarum</em> L.) leaves are widely processed as a healthy tea (GT) in China for their multiple biological activities. In this study, different polar segments from GT ethanolic extract were obtained using the liquid-liquid fractionation method. <em>n</em>-Butanol fraction (GN) had the highest antioxidant activity while ethyl acetate fraction (GE) showed the highest inhibitory effect on the <em>α</em>-glucosidase enzyme. The anti-diabetic effect of GE was further investigated in type 2 diabetic mellitus (T2DM) mice. The oral administration of GE (300 or 600 mg/kg) reduced fasting blood glucose levels, improved glucose tolerance ability, and ameliorated insulin resistance in T2DM mice. GE intervention could enhance the cholesterol transportation in the serum in coincidence with decreased total cholesterol, triglyceride levels, and non-esterified fatty acid. Transcriptomic, immunofluorescence and western blotting analysis revealed that GE significantly up-regulated insulin receptor substrate 1, phosphatidylinositol-3-kinase, protein kinase B, glycogen synthesis, and glucose transporter 4. AMP-activated protein kinases-mediate gluconeogenesis and glycogenolysis, such as phosphoenolpyruvate carboxy kinase1 and glucose-6-phosphatase in the liver and kidney tissues of GE groups were decreased. Thus, GE alleviated the liver, kidney, and pancreatic tissue damage. The phytoconstituents of GE were characterized, and rutin, neochlorogenic acid, and its acylated derivatives have hypoglycemic effects. Furthermore, these findings suggested that GT might be used as a promising hypoglycemic candidate.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 5","pages":"Pages 894-910"},"PeriodicalIF":7.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145718829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-27DOI: 10.1016/j.jfutfo.2024.11.012
Rong Huang , Yapeng Fang , Yu Zhong , Danfeng Wang , Wei Lu , Haiyun Zhao , Yun Deng
The quality of fermented vegetables is fundamentally driven by the microbiome. Although sequencing technologies have revealed patterns of microbial diversity in fermented vegetables across diverse geographic regions, production practices, and fermentation periods, the understanding of microbial community composition and interaction dynamics remains incomplete. Furthermore, achieving precise control over the fermentation process is still challenging. This review examines the current state of microbial succession patterns in kimchi, paocai, suansun fermented mustard and cucumber, emphasizing critical challenges in microbial control and identifying key factors influencing community dynamics, including synergistic and competitive interactions. It also presents emerging technologies in microbial spoilage prevention, aiming to enhance microbiome-informed process control. Additionally, the review assesses metabolic pathways and sensory characteristics of fermented vegetables and highlights health risks associated with compounds like sodium nitrite, biogenic amines, and harmful microorganisms. The integration of synthetic functional microbial communities is discussed as a promising approach to improve fermentation quality. Finally, the potential for digital tools such as machine learning and industrial robotics to standardize production processes and improve quality control is addressed, highlighting future directions and practical implications for the industry. Overall, these insights support a foundation for interdisciplinary research and sustainable development in the fermented vegetable industry.
{"title":"Advancing fermentation science: microbial dynamics, metabolomics, and safety in fermented vegetables","authors":"Rong Huang , Yapeng Fang , Yu Zhong , Danfeng Wang , Wei Lu , Haiyun Zhao , Yun Deng","doi":"10.1016/j.jfutfo.2024.11.012","DOIUrl":"10.1016/j.jfutfo.2024.11.012","url":null,"abstract":"<div><div>The quality of fermented vegetables is fundamentally driven by the microbiome. Although sequencing technologies have revealed patterns of microbial diversity in fermented vegetables across diverse geographic regions, production practices, and fermentation periods, the understanding of microbial community composition and interaction dynamics remains incomplete. Furthermore, achieving precise control over the fermentation process is still challenging. This review examines the current state of microbial succession patterns in kimchi, paocai, suansun fermented mustard and cucumber, emphasizing critical challenges in microbial control and identifying key factors influencing community dynamics, including synergistic and competitive interactions. It also presents emerging technologies in microbial spoilage prevention, aiming to enhance microbiome-informed process control. Additionally, the review assesses metabolic pathways and sensory characteristics of fermented vegetables and highlights health risks associated with compounds like sodium nitrite, biogenic amines, and harmful microorganisms. The integration of synthetic functional microbial communities is discussed as a promising approach to improve fermentation quality. Finally, the potential for digital tools such as machine learning and industrial robotics to standardize production processes and improve quality control is addressed, highlighting future directions and practical implications for the industry. Overall, these insights support a foundation for interdisciplinary research and sustainable development in the fermented vegetable industry.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 5","pages":"Pages 780-795"},"PeriodicalIF":7.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145718716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-27DOI: 10.1016/j.jfutfo.2024.09.008
Lili Zhang , Jialu Shi , Jinfang Sun , Yuxin Wang , Vijaya Raghavan , Jin Wang
The rising prevalence of food allergy is a global concern, especially as children are more susceptible to developing anaphylaxis than adults. Food-induced anaphylaxis, a severe and potentially life-threatening allergic reaction, imposes a healthcare burden in many Asian countries. While literature on food allergy in Asia is limited and heterogeneous, the prevalence shows an upward trend. In contrast, food allergy in developing countries is often overlooked as a health issue. China, with its vast population and landmass, is experiencing notable shifts in food allergy patterns. Unlike Western countries where tree nuts are common triggers, wheat, seafood, and fruits increasingly provoke allergic reactions among the Chinese population. Various diagnostic methods for food allergy were employed in China; however, the lack of a standardized approach presents challenges for effective management. In the future, it is essential to develop efficient and convenient detection methods while utilizing big data for extensive epidemiological investigations and clinical studies to address the complex health issue of food allergy.
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