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The role of ascorbate metabolism and cell wall degradation in atemoya peel browning: insights from multi-omics analysis under modified atmosphere packaging 抗坏血酸代谢和细胞壁降解在红豆果皮褐变中的作用:改良大气包装下多组学分析的见解
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-27 DOI: 10.1016/j.jfutfo.2024.12.006
Ruoyan Zhang , Yu Zhong , Danfeng Wang , Liang Gong , Feng Guo , Guoping Zhou , Yun Deng
Atemoya fruit, prized for their flavor and nutritional value, are susceptible to rapid postharvest deterioration, particularly peel browning, limiting their marketability. This browning process, often linked to oxidative stress and cell wall degradation, remains poorly understood at the molecular level. This study investigated the impact of modified atmosphere packaging (MAP) on atemoya peel browning during cold storage using integrated biochemical, metabolomic, and transcriptomic analyses. Results demonstrated that MAP effectively reduced browning index and extended shelf-life by 12 days. Multi-omics analysis revealed that MAP elevated antioxidant enzyme activities and ascorbic acid accumulation while delaying cell wall degradation. Notably, pectinesterase-encoding genes showed strong correlation with ascorbate and phenylpropanoid metabolism. These findings provide new insights into the molecular basis of MAP-mediated browning reduction in atemoya and suggest potential targets for postharvest quality improvement.
红豆果因其风味和营养价值而受到珍视,但采收后容易迅速变质,尤其是果皮变褐,限制了其市场价值。这种褐变过程通常与氧化应激和细胞壁降解有关,在分子水平上仍然知之甚少。本研究利用生化、代谢组学和转录组学综合分析研究了改良气调包装(MAP)对冷库中红豆果皮褐变的影响。结果表明,MAP能有效降低褐变指数,延长保质期12天。多组学分析显示,MAP可提高抗氧化酶活性和抗坏血酸积累,同时延缓细胞壁降解。值得注意的是,果胶酯酶编码基因与抗坏血酸和苯丙酸代谢密切相关。这些发现为揭示map介导的红豆褐变减少的分子基础提供了新的见解,并为采后品质改善提供了潜在的靶点。
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引用次数: 0
Tryptophan metabolism and the gut-brain axis: focus on specific gut microbial genera 色氨酸代谢和肠-脑轴:关注特定肠道微生物属
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-27 DOI: 10.1016/j.jfutfo.2024.09.006
Yuxuan Xia , Chuan Zhang , Leilei Yu , Qingsong Zhang , Arjan Narbad , Wei Chen , Qixiao Zhai , Fengwei Tian
The gut-brain axis (GBA) is a crucial connection that enables bidirectional communication between the central nervous system and gastrointestinal tract. Serotonin is a tryptophan (Trp) metabolite and an important gastrointestinal signaling molecule. Kynurenine and indole derivatives mediate the central nervous system, thereby affecting neurological diseases. The gut microbiota has direct or indirect impact on Trp metabolism. Different genera of gut microbiota affect Trp metabolites in different ways, thus changing the communication between the brain and gastrointestinal tract. This review highlights the triangular relationship between the GBA, Trp metabolism, and gut microbiota and summarizes the types of relevant gut microbiota genera involved in this triangular relationship. By modulating the gut microbiota through probiotics, dietary interventions, fecal microbiota transplantation, and other methods, improving Trp metabolism and thereby influencing metabolites provides potential targets for treating GBA-related diseases.
肠脑轴(GBA)是中枢神经系统和胃肠道之间双向交流的关键连接。5 -羟色胺是一种色氨酸代谢物,是一种重要的胃肠道信号分子。犬尿氨酸和吲哚衍生物介导中枢神经系统,从而影响神经系统疾病。肠道菌群对色氨酸代谢有直接或间接的影响。不同的肠道菌群以不同的方式影响色氨酸代谢物,从而改变大脑与胃肠道之间的交流。本文重点介绍了GBA、色氨酸代谢和肠道微生物群之间的三角关系,并总结了参与这种三角关系的相关肠道微生物群属的类型。通过益生菌、饮食干预、粪便菌群移植等方法调节肠道菌群,改善色氨酸代谢从而影响代谢物,为治疗gba相关疾病提供了潜在靶点。
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引用次数: 0
Quantitative analysis of adulterated crop flours in Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) using near infrared spectrometry combined with back-propagation neural network algorithm 苦荞(Fagopyrum tataricum, L.)中掺假作物粉的定量分析Gaertn)采用近红外光谱法结合反向传播神经网络算法
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-27 DOI: 10.1016/j.jfutfo.2024.11.015
Yue Yu , Yinghui Chai , Yujie Yan , Zhongyang Ren , Jiating Zhao , Zhanming Li , Lin Chen
Tartary buckwheat flour is esteemed for its high nutritional value and price, yet it is susceptible to adulteration in the market. Near-infrared spectroscopy (NIRS), commonly utilized for nutrient content detection, has recently been applied to authenticate food products This study collected near-infrared spectral data from adulterated samples of whole wheat flour, oat flour, soybean flour, barley flour, and sorghum flour in tartary buckwheat. Utilizing partial least squares regression (PLSR), support vector regression (SVR), and back-propagation neural network (BPNN), we predicted the adulterant content in tartary buckwheat. The results demonstrated that the BPNN algorithm, with an determination coefficient of prediction exceeding 0.97 and root mean square error of prediction below 0.02, surpassed the PLSR and SVR models in predicting adulterated crop flour, showcasing superior accuracy and generalization capabilities. This integration of NIRS and BPNN proved effective for the quantitative analysis of crop flours in tartary buckwheat, exhibiting robust predictive performance and rapid detection of adulteration in agricultural products.
苦荞粉因其高营养价值和价格而备受推崇,但在市场上容易掺假。近红外光谱(NIRS)是一种常用的营养成分检测方法,近年来已被应用于食品的鉴定。本研究采集了苦荞麦中掺假的全麦粉、燕麦粉、大豆粉、大麦粉和高粱粉的近红外光谱数据。利用偏最小二乘回归(PLSR)、支持向量回归(SVR)和反向传播神经网络(BPNN)对苦荞中掺假物含量进行了预测。结果表明,BPNN算法预测的决定系数大于0.97,预测均方根误差小于0.02,优于PLSR和SVR模型,具有较好的预测精度和泛化能力。近红外光谱(NIRS)和bp神经网络(BPNN)的结合被证明对苦荞中作物面粉的定量分析是有效的,表现出强大的预测性能和对农产品掺假的快速检测。
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引用次数: 0
Study on the encapsulation mechanism of collagen hydrogel for apigenin 芹菜素胶原水凝胶包封机理的研究
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-27 DOI: 10.1016/j.jfutfo.2024.09.007
Wenya Meng, Yuru Wu, Qian Zhou, Jing Li, Xu Liu, Kang Liu
Poor stability and low solubility of flavonoids extremely limited their efficient application in functional foods. Hydrogel delivery systems have good stabilizing and enhancing effects on flavonoids. Apigenin has a variety of biological activities and can be used to treat various inflammatory conditions. However, its low water solubility significantly hinders its widespread applications. As an amphoteric electrolyte, collagen can form a hydrogel delivery system with varying loading ability under different concentrations and pH values. Therefore, five different collagen concentrations and five different pH values were selected to investigate the optimal conditions for incorporating apigenin. Results found that when the collagen concentration was 5 mg/mL and the pH was 9, the collagen hydrogels had a good encapsulation effect for apigenin. The interaction between apigenin and collagen was primarily driven by hydrogen bonding and hydrophobic interactions. The binding of apigenin altered the conformation and microenvironment of collagen. Molecular docking analysis suggested that apigenin maintained binding stability in the hydrophobic pocket of proteins by interacting with several key residues. The experiments of in vitro digestion revealed the apigenin within that collagen hydrogels had good digestion stability. This study will provide a theoretical basis for the application of apigenin in the collagen delivery system.
黄酮类化合物稳定性差、溶解度低,极大地限制了其在功能食品中的有效应用。水凝胶给药系统对黄酮类化合物具有良好的稳定和增强作用。芹菜素具有多种生物活性,可用于治疗各种炎症。但其水溶性较低,严重阻碍了其广泛应用。胶原蛋白作为两性电解质,在不同浓度和pH值下可形成具有不同负载能力的水凝胶递送体系。因此,选择5种不同的胶原浓度和5种不同的pH值来研究芹菜素的最佳掺入条件。结果发现,当胶原浓度为5 mg/mL, pH为9时,胶原水凝胶对芹菜素具有良好的包封效果。芹菜素与胶原蛋白的相互作用主要是由氢键和疏水相互作用驱动的。芹菜素的结合改变了胶原的构象和微环境。分子对接分析表明,芹菜素通过与几个关键残基相互作用,在疏水蛋白口袋中保持了结合的稳定性。体外消化实验表明,胶原水凝胶中的芹菜素具有良好的消化稳定性。本研究将为芹菜素在胶原传递系统中的应用提供理论依据。
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引用次数: 0
Investigation of beetroot (Beta vulgaris L.) water-soluble fractions in altering yogurt properties 甜菜根(Beta vulgaris L.)水溶性组分对酸奶特性影响的研究
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-26 DOI: 10.1016/j.jfutfo.2024.11.017
Posman Sibuea , Dewi Restu Sihombing , Connie Daniela , Lumban Siantar V. Natalia , Joncer Naibaho
Different beetroot derivatives have been used in yogurt, improved the nutritional-related properties, and challenged the functionality as well as acceptability. However, fresh water-soluble-fraction in beetroot (WFB) in yogurt has never been reported. The study evaluated the functionality and acceptability of yogurt enriched with WFB at different concentrations (10, 20, and 30 g/L), which were evaluated at 5 °C for 0, 6, 12, and 18 days of storage. Compared to control, the addition of WFB significantly decreased the pH from 4.1–4.9 to 2.3–4.2 and increased total acid content from 2.1%–2.5% to 3.0%–3.6%, total lactic acid bacteria (LAB) count from 6.6–6.9 to 6.8–7.1 (lg(CFU/mL)), vitamin C from 2.3–3.1 to 2.4–4.1 mg/100 g, DPPH radical scavenging activity from 23.5%–50.0 % to 61%–69%, and hedonic acceptability. Total LAB count rose from 6.6 (lg(CFU/mL)) to reach the highest at 30 g/L addition which ranged of 6.8–7.1 (lg(CFU/mL)). 20 g/L of WFB generated a more stable vitamin C during the 18 days of storage period. During the 18 days of storage, the 20 and 30 g/L of WFB yogurt had a higher stability of antioxidants. In conclusion, 20 and 30 g/L of WFB can be expected to enhance the stability of nutritional-related properties of the yogurt with a higher acceptability. Low amount of WFB contributed to an attractive performance from consumer perspective.
不同的甜菜根衍生物被用于酸奶中,改善了酸奶的营养特性,并对酸奶的功能性和可接受性提出了挑战。然而,酸奶中甜菜根的新鲜水溶性成分(WFB)从未被报道过。该研究评估了不同浓度(10、20和30 g/L)富含WFB的酸奶的功能和可接受性,并在5°C下进行了0、6、12和18天的储存。与对照组相比,添加WFB显著降低了饲料的pH值从4.1 ~ 4.9降低到2.3 ~ 4.2,使总酸含量从2.1% ~ 2.5%提高到3.0% ~ 3.6%,使乳酸菌总数从6.6 ~ 6.9增加到6.8 ~ 7.1 (lg(CFU/mL)),使维生素C从2.3 ~ 3.1增加到2.4 ~ 4.1 mg/100 g,使DPPH自由基清除率从23.5% ~ 50.0%提高到61% ~ 69%,提高了饲料的可接受性。总LAB计数从6.6 (lg(CFU/mL))上升到30 g/L时最高,为6.8 ~ 7.1 (lg(CFU/mL))。20 g/L的WFB在18 d的贮藏期内产生了更稳定的维生素C。贮藏18 d时,20和30 g/L WFB酸奶抗氧化剂的稳定性较高。综上所述,添加20和30 g/L WFB可提高酸奶营养相关特性的稳定性,可接受性较高。从消费者的角度来看,低量的WFB有助于具有吸引力的性能。
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引用次数: 0
Ozonised water treatment delays ripening and senescence of stored papaya fruit via eliciting antioxidant defence system 臭氧水处理通过激发抗氧化防御系统延缓贮藏木瓜果实的成熟和衰老
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-24 DOI: 10.1016/j.jfutfo.2024.10.006
B.R. Vinod , Ram Asrey , Eldho Varghese , Nirmal Kumar Meena , V.B. Patel , Menaka M , Sajeel Ahamad
Fruit senescence profoundly affects the postharvest quality of papaya, which is linked to oxidative damage from reactive oxygen species and lipid peroxidation during storage. In this study, we analysed the effect and physiological mechanism of ozonised water (2 mg/L) dipping for 5 min on the antioxidant defence system and oxidative membrane damage in extending the shelf life of papaya fruit under cold storage. Ozonised water treatment inhibited respiratory metabolism and ethylene evolution, delayed the ripening and color indices, reduced peel browning and the loss of titratable acidity, and lowered disease severity. Additionally, ozonised water led to substantial elevations in total phenol, flavonoid, radical scavenging activity and antioxidant enzyme activities while mitigating oxidative breakdown by lowering the malondialdehyde and hydrogen peroxide content. Principal component analysis and Pearson correlation (multivariate analysis) further confirmed the effectiveness of ozonised water (2 mg/L) treatment in delaying the senescence of papaya by eliciting antioxidant defence system.
果实衰老严重影响木瓜采后品质,这与贮藏过程中活性氧的氧化损伤和脂质过氧化有关。本研究分析了2 mg/L臭氧水浸泡5 min对延长番木瓜果实冷藏保鲜期的抗氧化防御系统和氧化膜损伤的影响及其生理机制。臭氧水处理抑制了呼吸代谢和乙烯释放,延缓了成熟和颜色指数,减少了果皮褐变和可滴定酸度的损失,降低了病害严重程度。此外,臭氧水导致总酚、类黄酮、自由基清除活性和抗氧化酶活性的显著升高,同时通过降低丙二醛和过氧化氢含量来减轻氧化分解。主成分分析和Pearson相关性(多变量分析)进一步证实了臭氧水(2mg /L)处理通过激发抗氧化防御系统延缓木瓜衰老的有效性。
{"title":"Ozonised water treatment delays ripening and senescence of stored papaya fruit via eliciting antioxidant defence system","authors":"B.R. Vinod ,&nbsp;Ram Asrey ,&nbsp;Eldho Varghese ,&nbsp;Nirmal Kumar Meena ,&nbsp;V.B. Patel ,&nbsp;Menaka M ,&nbsp;Sajeel Ahamad","doi":"10.1016/j.jfutfo.2024.10.006","DOIUrl":"10.1016/j.jfutfo.2024.10.006","url":null,"abstract":"<div><div>Fruit senescence profoundly affects the postharvest quality of papaya, which is linked to oxidative damage from reactive oxygen species and lipid peroxidation during storage. In this study, we analysed the effect and physiological mechanism of ozonised water (2 mg/L) dipping for 5 min on the antioxidant defence system and oxidative membrane damage in extending the shelf life of papaya fruit under cold storage. Ozonised water treatment inhibited respiratory metabolism and ethylene evolution, delayed the ripening and color indices, reduced peel browning and the loss of titratable acidity, and lowered disease severity. Additionally, ozonised water led to substantial elevations in total phenol, flavonoid, radical scavenging activity and antioxidant enzyme activities while mitigating oxidative breakdown by lowering the malondialdehyde and hydrogen peroxide content. Principal component analysis and Pearson correlation (multivariate analysis) further confirmed the effectiveness of ozonised water (2 mg/L) treatment in delaying the senescence of papaya by eliciting antioxidant defence system.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 5","pages":"Pages 870-880"},"PeriodicalIF":7.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145718724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Subcritical water extraction of bioactive compounds from plant materials: recent advances 亚临界水萃取植物原料中生物活性化合物的研究进展
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-24 DOI: 10.1016/j.jfutfo.2024.10.005
Husna Abdul Somat , Nurfatimah Mohd Thani , Wan Aida Wan Mustapha , Seng Joe Lim , Noor Soffalina Sofian Seng , Hafeedza Abdul Rahman , Noorul Syuhada Mohd Razali , Maimunah Mohd Ali , Siti Mazlina Mustapa Kamal
Bioactive compounds play a crucial role in various industries. Natural plants are abundant sources of these compounds, spanning diverse chemical classes such as polyphenols, alkaloids, and terpenoids, each offering unique therapeutic properties. Efficient extraction of these compounds from natural plant sources is essential, yet challenging due to the variability in compound distribution, matrix complexity, and the need for environmentally sustainable practices. Subcritical water extraction (SWE) has emerged as a promising green technology for extracting bioactive compounds from natural sources, utilizing water as a solvent to obviate environmental and health risks associated with organic solvents. SWE enables simultaneous extraction of both polar and non-polar compounds, offering versatility for diverse phytochemical profiles. Recent studies have shown that SWE can effectively extract a wide range of bioactive compounds and improved compound purity compared to other traditional mothed. Its principles and mechanisms highlight advantages over conventional methods, notably reduced organic solvent dependence, shorter extraction durations, and heightened selectivity. However, despite its many advantages, challenges remain. These include optimizing extraction parameters for different plant matrices, ensuring scalability for industrial applications, and addressing the thermal sensitivity of certain bioactive compounds under high temperatures. Furthermore, equipment costs and process optimization for large-scale extractions still pose limitations. Nonetheless, SWE’s ability to minimize environmental impact while enhancing extraction efficiency makes it a compelling alternative to conventional techniques. This manuscript will focus on the recent advancements in SWE for plant extraction which underscores the importance of ongoing research to refine extraction protocols, scale-up processes, and explore potential synergies by integrating SWE with complementary methods or modifications. Ultimately, this review discusses the efficacy of SWE as a sustainable and efficient approach for extracting bioactive compounds from plants, offering invaluable guidance to researchers and industries engaged in natural product extraction, thereby facilitating enhanced utilization of botanical resources.
生物活性化合物在各行各业中发挥着至关重要的作用。天然植物是这些化合物的丰富来源,跨越不同的化学类别,如多酚、生物碱和萜类,每一种都提供独特的治疗特性。从天然植物中高效提取这些化合物至关重要,但由于化合物分布的可变性、基质的复杂性以及对环境可持续实践的需求,这一过程具有挑战性。亚临界水萃取(SWE)已成为一种有前途的绿色技术,用于从天然资源中提取生物活性化合物,利用水作为溶剂,以避免与有机溶剂相关的环境和健康风险。SWE可以同时提取极性和非极性化合物,为不同的植物化学特征提供多功能性。近年来的研究表明,与其他传统方法相比,SWE可以有效地提取广泛的生物活性化合物,并提高化合物纯度。其原理和机制突出了传统方法的优点,特别是减少了对有机溶剂的依赖,缩短了提取时间,提高了选择性。然而,尽管它有许多优点,挑战仍然存在。这些包括优化不同植物基质的提取参数,确保工业应用的可扩展性,以及解决某些生物活性化合物在高温下的热敏性。此外,大规模提取的设备成本和工艺优化仍然存在局限性。尽管如此,SWE能够在提高提取效率的同时最大限度地减少对环境的影响,使其成为传统技术的一个令人信服的替代方案。本文将重点介绍SWE用于植物提取的最新进展,强调正在进行的研究的重要性,以改进提取方案,扩大工艺规模,并通过将SWE与互补方法或修改相结合来探索潜在的协同作用。最后,本文讨论了SWE作为一种可持续、高效的植物活性化合物提取方法的功效,为从事天然产物提取的研究人员和行业提供了宝贵的指导,从而促进植物资源的有效利用。
{"title":"Subcritical water extraction of bioactive compounds from plant materials: recent advances","authors":"Husna Abdul Somat ,&nbsp;Nurfatimah Mohd Thani ,&nbsp;Wan Aida Wan Mustapha ,&nbsp;Seng Joe Lim ,&nbsp;Noor Soffalina Sofian Seng ,&nbsp;Hafeedza Abdul Rahman ,&nbsp;Noorul Syuhada Mohd Razali ,&nbsp;Maimunah Mohd Ali ,&nbsp;Siti Mazlina Mustapa Kamal","doi":"10.1016/j.jfutfo.2024.10.005","DOIUrl":"10.1016/j.jfutfo.2024.10.005","url":null,"abstract":"<div><div>Bioactive compounds play a crucial role in various industries. Natural plants are abundant sources of these compounds, spanning diverse chemical classes such as polyphenols, alkaloids, and terpenoids, each offering unique therapeutic properties. Efficient extraction of these compounds from natural plant sources is essential, yet challenging due to the variability in compound distribution, matrix complexity, and the need for environmentally sustainable practices. Subcritical water extraction (SWE) has emerged as a promising green technology for extracting bioactive compounds from natural sources, utilizing water as a solvent to obviate environmental and health risks associated with organic solvents. SWE enables simultaneous extraction of both polar and non-polar compounds, offering versatility for diverse phytochemical profiles. Recent studies have shown that SWE can effectively extract a wide range of bioactive compounds and improved compound purity compared to other traditional mothed. Its principles and mechanisms highlight advantages over conventional methods, notably reduced organic solvent dependence, shorter extraction durations, and heightened selectivity. However, despite its many advantages, challenges remain. These include optimizing extraction parameters for different plant matrices, ensuring scalability for industrial applications, and addressing the thermal sensitivity of certain bioactive compounds under high temperatures. Furthermore, equipment costs and process optimization for large-scale extractions still pose limitations. Nonetheless, SWE’s ability to minimize environmental impact while enhancing extraction efficiency makes it a compelling alternative to conventional techniques. This manuscript will focus on the recent advancements in SWE for plant extraction which underscores the importance of ongoing research to refine extraction protocols, scale-up processes, and explore potential synergies by integrating SWE with complementary methods or modifications. Ultimately, this review discusses the efficacy of SWE as a sustainable and efficient approach for extracting bioactive compounds from plants, offering invaluable guidance to researchers and industries engaged in natural product extraction, thereby facilitating enhanced utilization of botanical resources.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 5","pages":"Pages 753-764"},"PeriodicalIF":7.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145718714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Volatile Compounds in Weixian Radish Varieties using HS-GC-IMS with OPLD 魏县萝卜品种挥发性成分的hplc - gc - ims - OPLD分析
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-06-01 DOI: 10.1016/j.jfutfo.2025.02.005
Qianqian Jia, Yanrong Ma, Rui Zhang, Wenlu Bi, Deping Han, Sen Yang, Dingding Su
Radish is a root vegetable with significant nutritional and economic value. However, there is limited information on the flavor and taste profiles of different radish varieties. In this study, headspace gas chromatography–ion mobility mass spectrometry was used to investigate the characteristic volatile compounds in Weixian green radish, establishing its volatile compound fingerprint. Further, its volatile compound composition was compared with those of other radish varieties. A total of 46 compounds were identified in the Weixian green radish, including 19 aldehydes, six alcohols, five esters, six ketones, three furans, three pyrazines, and four other compounds. Among different radish varieties, the Weixian radish contained the largest number of volatile compounds, followed by hybrid and green-root radish samples. Flavor differences among the different radish varieties were mainly affected by the type and content of esters, aldehydes, and ketones, among which 1-nonanal, propanal, 1-penten-3-one, and isobutyric acid methyl ester were the main differential flavor substances. This study not only reveals the differences in the flavor compounds between different radish varieties, but also provides a scientific basis for the geographical protection of varieties and market positioning of radishes in the Weixian county.
萝卜是一种具有重要营养价值和经济价值的块根蔬菜。然而,关于不同萝卜品种的风味和口感特征的信息有限。本研究采用顶空气相色谱-离子迁移率质谱法对潍县青萝卜的挥发性化合物特征进行了研究,建立了其挥发性化合物指纹图谱。并对其挥发物组成进行了比较。从潍县青萝卜中共鉴定出46种化合物,包括19种醛类、6种醇类、5种酯类、6种酮类、3种呋喃类、3种吡嗪类和4种其他化合物。不同萝卜品种中挥发性化合物含量最高的是潍县萝卜,其次是杂交萝卜和绿根萝卜。不同品种萝卜的风味差异主要受酯类、醛类和酮类的类型和含量的影响,其中1-壬醛、丙醛、1-戊-3-酮和异丁酸甲酯是主要的风味差异物质。本研究不仅揭示了不同萝卜品种间风味化合物的差异,而且为潍县萝卜品种的地理保护和市场定位提供了科学依据。
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引用次数: 0
Effect of non-starter lactobacilli used as adjunct-culture in model cheese production on volatile compounds and free amino acid contents 非发酵剂乳酸菌辅助培养对模型奶酪挥发性化合物和游离氨基酸含量的影响
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-05-26 DOI: 10.1016/j.jfutfo.2025.05.001
Didem Şahingil, Yasemin Gökçe, Ali Adnan Hayaloğlu
Adjunct culture can be used to improve typical cheese aroma and contribute to proteolysis during ripening process. In the present study, some lactobacilli (Lactobacillus casei, L. delbrueckii subsp. bulgaricus, Lactiplantibacillus plantarum and two strains of L. helveticus) were tested in model cheeses during 120 days of ripening at the temperatures of 8 °C and 16 °C. It was aimed to investigate the effects of these parameters on free amino acid (FAA) content, volatile aroma profile and sensory properties in cheese. Volatile compounds in cheese samples were determined by gas chromatography/mass spectrometry (GC/MS) analysis and FAA content by high performance liquid chromatography (HPLC) analysis. Principal component (PCA), partial least squares-discriminant analysis (sPLSDA) and heat map cluster analysis were performed to distinguish different volatile aroma and FAAs in cheeses according to ripening time and temperature as well as adjunct culture. The predominant FAAs in the cheese were Arg, Tyr, Val, Leu, Trp and Lys, regardless of ripening. The highest concentration of Glu was observed in cheeses containing L. bulgaricus, followed by cheeses with L. helveticus and L. plantarum. A total of 41 volatile compounds were identified, with variations noted between the different cheeses in particular L. helveticus, play a crucial role in flavor development. The ripening temperature also influenced the production of volatile compounds: At 16 °C, cheeses with L. bulgaricus and L. helveticus were characterized by alcohols, esters and acids, while cheeses with L. plantarum contained mainly, acids, aldehydes and ketones. For actobacilli, especially combination of L. helveticus, L. bulgaricus and L. plantarum, can be added to the cheese to improve and intensify the flavor. It was seen that the positive effects of selected adjunct cultures on aroma and taste of model cheeses were related to volatiles and FAAs.
辅助培养可以改善典型奶酪的香气,并有助于成熟过程中的蛋白质水解。在本研究中,一些乳酸菌(干酪乳杆菌,德尔布鲁氏乳杆菌亚种)。在8°C和16°C的温度下,在120天的成熟过程中,在模型奶酪中测试了保加利亚乳杆菌、植物乳杆菌和两株helveticus。研究了这些参数对奶酪游离氨基酸(FAA)含量、挥发性香气特征和感官特性的影响。采用气相色谱/质谱(GC/MS)分析和高效液相色谱(HPLC)分析奶酪样品中的挥发性化合物和FAA含量。采用主成分分析(PCA)、偏最小二乘判别分析(sPLSDA)和热图聚类分析等方法,根据成熟度、温度和辅助培养对奶酪挥发性香气和FAAs进行区分。无论成熟度如何,奶酪中的主要FAAs为Arg、Tyr、Val、Leu、Trp和Lys。其中,保加利亚乳杆菌含量最高,helveticus乳杆菌次之,植物乳杆菌次之。共鉴定出41种挥发性化合物,不同奶酪之间存在差异,特别是L. helveticus,在风味形成中起着至关重要的作用。成熟温度对挥发性化合物的产生也有影响:在16℃时,保加利亚乳杆菌和helveticus乳杆菌的奶酪主要含有醇类、酯类和酸类,而plantarum乳杆菌的奶酪主要含有酸类、醛类和酮类。对于乳酸菌,尤其是helveticus、L. bulgaricus和L. plantarum的组合,可以在奶酪中添加乳酸菌来改善和强化奶酪的风味。结果表明,所选择的辅助培养物对模型奶酪香气和口感的积极影响与挥发物和FAAs有关。
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引用次数: 0
Investigation on Flavor Compounds, Antioxidant Properties, and Sensory Attributes in Pear Juices 梨汁风味化合物、抗氧化特性及感官特性的研究
IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-05-26 DOI: 10.1016/j.jfutfo.2025.03.001
Hong Cheng , Anwei Yi , Yudou Cheng , Yiming Li , Junfeng Guan
The nutritional composition of pear juice, including its flavor profile and bioactive compounds, plays a pivotal role in determining its quality and is significantly influenced by the pear cultivar. This study assessed key flavor compounds, including sugars, organic acids, and volatiles, as well as antioxidant properties (mainly consisting of phenolics, DPPH, and OH radical scavenging activity) and sensory attributes based on electronic tongue and nose technologies, of pear juices across 20 cultivars from four pear species. The results demonstrated a consistent relationship between flavor compounds and sensory attributes. The total sugar content was positively associated with sweetness perception, with P. bretschneideri, P. pyrifolia, and P. sinkiangensis exhibiting higher sugar levels than P. ussuriensis. Similarly, total organic acid content corresponded to sourness perception, following the trend: P. ussuriensis > P. bretschneideri > P. pyrifolia and P. sinkiangensis. Notably, P. ussuriensis juices displayed higher levels of total volatile compounds, especially for esters, as confirmed by electronic nose analysis identifying a distinct volatile profile for this species. Additionally, Principal Component Analysis highlighted that P. ussuriensis juices were characterized by higher levels of total acids, citric acid, malic acid, esters, and antioxidant properties, along with reduced total sugars, glucose, and fructose levels. Partial Least Squares-Discriminant Analysis further screened ten differential variables contributing to group differentiation. These results indicated P. ussuriensis juices possessed distinctive characteristics of higher sourness, lower sweetness, rich aroma, and potential health benefits, which will provide foundational information to support the development of the pear juice industry in China.
梨汁的营养成分,包括其风味特征和生物活性成分,在决定其品质方面起着关键作用,并受到梨品种的显著影响。本研究评估了来自4个梨品种的20个品种的梨汁的主要风味化合物,包括糖、有机酸和挥发物,以及抗氧化性能(主要包括酚类物质、DPPH和•OH自由基清除活性)和基于电子舌头和鼻子技术的感官属性。结果表明,风味化合物和感官属性之间存在一致的关系。总糖含量与甜度感知呈正相关,P. bretschneideri、P. pyrifolia和P. sinkiangensis的糖含量高于P. ussuriensis。同样,总有机酸含量与酸味感知相对应,趋势如下:P. ussuriensis >; P. bretschneideri >; P. pyrifolia和P. sinkiangensis。值得注意的是,乌苏里纸种果汁显示出更高水平的总挥发性化合物,特别是酯类,这一点通过电子鼻分析证实了,该物种具有独特的挥发性特征。此外,主成分分析强调,乌苏里纸种果汁具有较高水平的总酸、柠檬酸、苹果酸、酯和抗氧化性能,同时总糖、葡萄糖和果糖水平降低。偏最小二乘-判别分析进一步筛选了10个有助于群体分化的微分变量。上述结果表明,乌苏里梨汁具有高酸、低甜、香气浓郁的特点,具有潜在的健康益处,为支持中国梨汁产业的发展提供了基础信息。
{"title":"Investigation on Flavor Compounds, Antioxidant Properties, and Sensory Attributes in Pear Juices","authors":"Hong Cheng ,&nbsp;Anwei Yi ,&nbsp;Yudou Cheng ,&nbsp;Yiming Li ,&nbsp;Junfeng Guan","doi":"10.1016/j.jfutfo.2025.03.001","DOIUrl":"10.1016/j.jfutfo.2025.03.001","url":null,"abstract":"<div><div>The nutritional composition of pear juice, including its flavor profile and bioactive compounds, plays a pivotal role in determining its quality and is significantly influenced by the pear cultivar. This study assessed key flavor compounds, including sugars, organic acids, and volatiles, as well as antioxidant properties (mainly consisting of phenolics, DPPH, and <sup>•</sup>OH radical scavenging activity) and sensory attributes based on electronic tongue and nose technologies, of pear juices across 20 cultivars from four pear species. The results demonstrated a consistent relationship between flavor compounds and sensory attributes. The total sugar content was positively associated with sweetness perception, with <em>P. bretschneideri, P. pyrifolia</em>, and <em>P. sinkiangensis</em> exhibiting higher sugar levels than <em>P. ussuriensis</em>. Similarly, total organic acid content corresponded to sourness perception, following the trend: <em>P. ussuriensis</em> &gt; <em>P. bretschneideri</em> &gt; <em>P. pyrifolia</em> and <em>P. sinkiangensis</em>. Notably, <em>P. ussuriensis</em> juices displayed higher levels of total volatile compounds, especially for esters, as confirmed by electronic nose analysis identifying a distinct volatile profile for this species. Additionally, Principal Component Analysis highlighted that <em>P. ussuriensis</em> juices were characterized by higher levels of total acids, citric acid, malic acid, esters, and antioxidant properties, along with reduced total sugars, glucose, and fructose levels. Partial Least Squares-Discriminant Analysis further screened ten differential variables contributing to group differentiation. These results indicated <em>P. ussuriensis</em> juices possessed distinctive characteristics of higher sourness, lower sweetness, rich aroma, and potential health benefits, which will provide foundational information to support the development of the pear juice industry in China.</div></div>","PeriodicalId":100784,"journal":{"name":"Journal of Future Foods","volume":"6 4","pages":"Pages 656-667"},"PeriodicalIF":7.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145334513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Journal of Future Foods
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