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A review of meroterpenoids and of their bioactivity from guava (Psidium guajava L.) 番石榴(Psidium guajava L.)中萜类化合物及其生物活性研究进展
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.005
Xinfeng Zou , Haiyang Liu

Psidium guajava Linn. (family Myrtaceae), is commonly known as guava. Guava is consumed in large quantities throughout the world due to its ease of cultivation and high nutritional value. As an important perennial fruit tree distributed around the world, guava has been widely used to manage various diseases in traditional medicine. Guava has been shown significant bioactivity such as antioxidant, anti-inflammatory, immunomodulatory, antimicrobial, antidiabetic, and anticancer properties. A large number of studies indicated that guava contains various type of phytochemicals including monoterpenes, sesquiterpenes, triterpenes, phenolics, and meroterpenoids. Among them, meroterpenoids are characteristic components of guava, which are hybrid of acylphloroglucinol with terpenoids (monoterpenes, sesquiterpenes). Modern pharmacological investigations showed intricate Psidium meroterpenoids possess a wide range of bioactivities. Although a large and growing body of literatures have investigated the Psidium meroterpenoids isolated from guava, a comprehensive review of Psidium meroterpenoids and their bioactivities has not been conducted. Therefore, this review provides a comprehensive compile of 75 meroterpenoids isolated from guava and their bioactivities between 2007 and May 2022. Furthermore, the possible biosynthesis way and future directions of Psidium meroterpenoids have also been discussed.

番石榴。(杨梅科),俗称番石榴。番石榴因其易于种植和营养价值高而在世界各地被大量食用。番石榴作为分布在世界各地的重要多年生果树,在传统医学中被广泛用于治疗各种疾病。番石榴已显示出显著的生物活性,如抗氧化、抗炎、免疫调节、抗菌、抗糖尿病和抗癌特性。大量研究表明,番石榴含有多种植物化学物质,包括单萜、倍半萜、三萜、酚类和亚萜类。其中,亚萜类化合物是番石榴的特征成分,是酰基氯三酚与萜类化合物(单萜、倍半萜)的杂交产物。现代药理学研究表明,复杂的Psidium亚萜类化合物具有广泛的生物活性。尽管越来越多的文献对番石榴中分离的Psidium亚萜类化合物进行了研究,但尚未对Psdium亚萜及其生物活性进行全面的综述。因此,这篇综述提供了2007年至2022年5月期间从番石榴中分离的75种亚萜类化合物及其生物活性的综合汇编。此外,还对Psidium亚萜类化合物可能的生物合成途径和未来的发展方向进行了讨论。
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引用次数: 0
Protein-polysaccharide nanocomplexes as nanocarriers for delivery of curcumin: a comprehensive review on preparation methods and encapsulation mechanisms 蛋白质-多糖纳米复合物作为姜黄素的纳米载体:制备方法和包封机理综述
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.002
Jingyi Xue, Yangchao Luo

Curcumin, commonly used as a coloring agent as well as food additive, has exhibited great therapeutic efficacy for development of functional foods. In order to expand its applications in food, strategies are needed to tackle the poor water-solubility, bioavailability and bioaccessibility of curcumin. Nanoparticles made from individual biomaterials such as proteins, polysaccharides and lipids have been introduced and their properties have been comprehensively studied. Recently, there has been an increasing interest in nanocomplexes made from two or more biomaterials because of their ability to composite the advantages of various components. This review has specific focus on the nanocomplexes fabricated from proteins and polysaccharides. First, complexation methods with or without chemical covalent bond formation are summarized, while different methods with different degrees of complexation are discussed. The encapsulation strategies of curcumin, both traditional and newly invented, are also compared. Then, the structure and physicochemical properties of the resulting binary or ternary nanocomplexes are discussed in detail, as well as a comparison of the delivery efficiency. Last but not least, the current limitations and future directions in the development of curcumin-loaded biopolymer nanoparticles for the design of future food products are presented.

姜黄素作为一种常用的着色剂和食品添加剂,在功能性食品的开发中表现出了巨大的疗效。为了扩大其在食品中的应用,需要采取策略来解决姜黄素的水溶性、生物利用度和生物可及性差的问题。介绍了由蛋白质、多糖和脂质等生物材料制成的纳米颗粒,并对其性质进行了全面研究。最近,人们对由两种或多种生物材料制成的纳米复合物越来越感兴趣,因为它们能够复合各种成分的优点。这篇综述的重点是由蛋白质和多糖制备的纳米复合物。首先,总结了有或没有化学共价键形成的络合方法,并讨论了不同络合程度的不同方法。并对姜黄素的传统和新发明的包封策略进行了比较。然后,详细讨论了所得二元或三元纳米配合物的结构和物理化学性质,以及递送效率的比较。最后但并非最不重要的是,介绍了负载姜黄素的生物聚合物纳米颗粒在设计未来食品方面的当前局限性和未来方向。
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引用次数: 12
Future foods, dietary factors and healthspan 未来的食物,饮食因素和健康跨度
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.001
Kaiqiang Li , Chong Wang , Yanbo Wang , Linglin Fu , Nianshu Zhang

Ageing is a universal decline of physiological functions accompanied by an increase in risks of developing morbidity, diseases, and death. Calorie restriction (CR) without malnutrition has been shown to improve lifespan from simple model organisms to mammals, and extensive research over the past decades have identified several universally conserved signalling pathways by which CR regulates lifespan. More recently, emerging evidence has suggested that modulation of intake levels of macronutrients and micronutrients can also impact healthspan and lifespan in model organisms. These findings propose potentially promising and cost-effective approaches to promote healthy ageing and longevity in humans through personalised nutrition. In this review, we summarise the mechanisms by which CR promotes healthspan and longevity, focusing on the mitochondrial reactive oxygen species (ROS) and several universally conserved geroprotective nutrient-sensing pathways (insulin/insulin-like growth factor (IGF-1), AMP-activated protein kinase (AMPK), mTOR). We further discuss the accumulating data supporting that changes in dietary pattern, levels of nutrient intake (both macronutrient and micronutrient) and functional foods can impact healthspan through acting on the key components of nutrient-sensing and immunoprotective pathways, providing fundamental support for future research and development of anti-ageing diets and dietary regimes.

衰老是一种普遍的生理功能下降,伴随着发病、疾病和死亡风险的增加。从简单的模式生物到哺乳动物,在没有营养不良的情况下限制热量(CR)已被证明可以延长寿命,过去几十年的广泛研究已经确定了CR调节寿命的几种普遍保守的信号通路。最近,新出现的证据表明,调节大量营养素和微量营养素的摄入水平也会影响模式生物的健康寿命。这些发现提出了通过个性化营养促进人类健康衰老和长寿的潜在前景和成本效益高的方法。在这篇综述中,我们总结了CR促进健康和寿命的机制,重点是线粒体活性氧(ROS)和几种普遍保守的促性腺激素保护营养传感途径(胰岛素/胰岛素样生长因子(IGF-1)、AMP活化蛋白激酶(AMPK)、mTOR)。我们进一步讨论了不断积累的数据,这些数据支持饮食模式、营养摄入水平(包括常量营养素和微量营养素)和功能性食品的变化可以通过作用于营养传感和免疫保护途径的关键成分来影响健康,为未来抗衰老饮食和饮食制度的研究和开发提供基本支持。
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引用次数: 2
Chemical constituents and coagulation effects of the flowers of Rosa chinensis Jacq. 月季花的化学成分及凝血作用。
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.006
Peiqing Wang , Miyun Hu , Li Wang , Jiaojiao Qu , Yuhang Liu , Changqin Li , Zhenhua Liu , Changyang Ma , Wenyi Kang

In this paper, the flowers of Rosa chinensis Jacq. were investigated and 14 compounds were isolated and identified, namely kaempferol (1), quercetin (2), isoquercitrin (3), afzelin (4), quercitrin (5), phenylethyl glucopyranoside (6), avicularin (7), juglanin (8), nicotiflorin (9), phenylethyl-6′-O-galloyl-β-D-glucopyranoside (10), tiliroside (11), methyl gallate (12), 8-O-methylherbacetin-3-O-β-D-sophoroside (13), gallic acid (14). Among these compounds, compounds 7, 9, 12 and 13 were isolated from R. chinensis for the first time. These compounds and extracts of R. chinensis. were studied for coagulation activity in vitro. The results showed that tiliroside (11) had good effect on promoting blood coagulation, and the effect of tiliroside was better than that of Yunnanbaiyao. Juglanin (8) and nicotiflorin (9) could significantly shorten thrombin time (TT) and significantly elevated fibrinogen (FIB), which proved that juglanin and nicotiflorin had good procoagulant effect.

本文对中国蔷薇的花进行了研究。对14个化合物进行了分离鉴定,分别为山奈酚(1)、槲皮素(2)、异槲皮素(3)、阿夫泽林(4)、槲皮苷(5)、苯乙基吡喃葡糖苷(6。在这些化合物中,化合物7、9、12和13是第一次从R.chinensis中分离得到。这些化合物和R.chinensis的提取物。研究了体外凝血活性。结果表明,tililoside(11)具有良好的促凝血作用,且tililosid的促凝血效果优于云南白药。Juglanin(8)和nicotiflorin(9)可显著缩短凝血酶时间(TT),显著升高纤维蛋白原(FIB),证明Juglanin和nicotifrorin具有良好的促凝作用。
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引用次数: 0
The untapped potential of zeolites in techno-augmentation of the biomaterials and food industrial processing operations: a review 沸石在生物材料技术增强和食品工业加工操作中未开发的潜力:综述
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.004
Piyush Sharma , Parag Prakash Sutar , Hongwei Xiao , Qi Zhang

Zeolites are aluminosilicate compounds with excellent selective, precise adsorption capabilities that find their potential applications in various sectors of the biomaterials and food industry. The zeolites from ages have been utilized in biomedicine, catalysis, detergent and air purification industries but their commercial applications in the biomaterials and food industry are not studied enough. Zeolites are known to reduce mycotoxins, toxic compounds, heavy metals, and microbes. The present review tries to summarize different arenas of zeolite applications in the biomaterials and food industry. The article also includes a discussion of individual applications of zeolitic properties like antimicrobial effect, food nanoreactors and intelligent food contact behaviour in brief. Moreover, sound coverage is given over zeolite classification, their synthesis and the modelling of their adsorption behaviour. The outcomes of the review emphasize the commercial viability of zeolites and their potential to serve as a possible alternative to many processing steps which ultimately will economise the production process in the food industry. This review also emphasizes extensive research that needs to be carried out to further improvise the capabilities of zeolites to overcome the challenges lying around them.

沸石是一种铝硅酸盐化合物,具有优异的选择性和精确的吸附能力,在生物材料和食品工业的各个领域都有潜在的应用。沸石已被应用于生物医学、催化、洗涤剂和空气净化等行业,但在生物材料和食品工业中的商业应用研究还不够。众所周知,沸石可以减少真菌毒素、有毒化合物、重金属和微生物。本文综述了沸石在生物材料和食品工业中的不同应用领域。文章还简要讨论了沸石性质的各个应用,如抗菌效果、食品纳米反应器和智能食品接触行为。此外,还介绍了沸石的分类、合成及其吸附行为的建模。审查结果强调了沸石的商业可行性及其作为许多加工步骤的可能替代品的潜力,这些步骤最终将使食品行业的生产过程经济化。这篇综述还强调了需要进行的广泛研究,以进一步提高沸石的能力,克服其面临的挑战。
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引用次数: 7
Advances in strategies to assure the microbial safety of food-associated ice 确保与食品有关的冰的微生物安全策略的进展
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.003
Ning Wang , Yeru Wang , Li Bai , Xinyu Liao , Donghong Liu , Tian Ding

Ice has been extensively employed as a general coolant or ingredient in the food industry. However, evident indicates that food-associated ice can be easily contaminated by pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, and Salmonella spp., among others. The contamination sources are attributed to the use of unhygienic water, improper handling, and poor hygiene conditions of ice-making machines. The contaminated ice might be a vesicle for the transmission of pathogens to food and human, posing potential risks to public health. This review overviewed the microbial contamination of food-associated ice, including the microbial contamination levels and the main microbial contaminants. In addition, a systematical introduction was constructed on the recent advances and applications of state-of-the-art methods to manufacture safe ices, illustrating ozone, electrolyzed water, cold plasma, essential oils, and ultraviolet light-emitting diode irradiation. The validities and limitations of ice treated by these techniques on the food quality were then discussed in detail. Various studies were also organized to demonstrate the potential of the mentioned treated ice, for ensuring the microbial safety and control quality deterioration during the storage of aquatic products. Finally, the direction for future study was given.

冰已被广泛用作食品工业中的通用冷却剂或配料。然而,显而易见的是,与食物相关的冰很容易被致病菌污染,如大肠杆菌、金黄色葡萄球菌和沙门氏菌等。污染源是由于使用了不卫生的水、处理不当以及制冰机的卫生条件差。受污染的冰可能是病原体传播给食物和人类的囊泡,对公众健康构成潜在风险。本文综述了食品相关冰的微生物污染,包括微生物污染水平和主要微生物污染物。此外,还系统地介绍了最先进的安全冰制造方法的最新进展和应用,包括臭氧、电解水、冷等离子体、精油和紫外线发光二极管照射。然后详细讨论了这些技术处理的冰对食品质量的有效性和局限性。还组织了各种研究,以证明上述处理过的冰在确保微生物安全和控制水产品储存过程中质量恶化方面的潜力。最后给出了今后的研究方向。
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引用次数: 1
Jute seed bioactive compounds: amino acids, polyphenolics, antioxidants and hydrolyzing enzymes inhibitory property 黄麻种子生物活性化合物:氨基酸、多酚类物质、抗氧化剂和水解酶抑制特性
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.010
Oluwole Steve Ijarotimi , Opeyemi Rachael Fagoroye , Timilehin David Oluwajuyitan

The processed whole jute seed flour (JSF), protein isolate (JSI) and hydrolysate (JSH) were subjects to amino-acids, phytochemicals, phenolic profiles, antioxidant, and enzymes (pancreatic lipase, α-amylase and α-glucosidase) inhibitory properties analysis. Protein and in-vitro protein digestibility of the samples ranged were 10−66 g/100 g and 17.5%−20.8%, respectively. Essential, sulphur, aromatic, branched chain, antioxidant, and hydrophobic amino-acids concentrations varied from 30.97−34.74, 2.91−3.53, 11.50−12.49, 14.12−16.55, 17.84−20.39, and 36.59−42.41 g/100 g protein, respectively. Phytochemical concentrations were significantly different (P > 0.05). For phenolic profile, vanillic acid had the highest concentration followed by naringin acid. JSI exhibited highest antioxidant activity in respect to 1,1-diphenyl-2-picryl-hydrazyl (DPPH), Fe2+ chelation, OH radical, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing antioxidant power, and inhibitory properties against lipase (53.4%), α-amylase (31.8%) and α-glucosidase (43.8%) enzymes, while JSF had the least. In conclusion, JSI exhibited supreme scavenging activities and attenuation of lipase, and carbohydrate hydrolyzing enzymes. Hence, JSI may be suitable as therapeutic agent for the prevention/management of food related diseases instead of synthetics agents with many side effects.

对加工后的全黄麻籽粉(JSF)、分离蛋白(JSI)和水解产物(JSH)的氨基酸、植物化学物质、酚类物质、抗氧化剂和酶(胰脂肪酶、α-淀粉酶和α-葡萄糖苷酶)的抑制特性进行了分析。样品的蛋白质和体外蛋白质消化率分别为10−66 g/100 g和17.5%−20.8%。必需氨基酸、硫氨基酸、芳香氨基酸、支链氨基酸、抗氧化剂氨基酸和疏水氨基酸的浓度分别为30.97−34.74、2.91−3.53、11.50−12.49、14.12−16.55、17.84−20.39和36.59−42.41 g/100 g蛋白质。植物化学物质浓度差异显著(P>0.05),酚类物质浓度以香兰素酸最高,其次为柚皮苷酸。JSI对1,1-二苯基-2-苦基-肼(DPPH)、Fe2+螯合、OH自由基、2,2′-叠氮双-(3-乙基苯并噻唑啉-6-磺酸)和铁还原抗氧化能力的抗氧化活性最高,对脂肪酶(53.4%)、α-淀粉酶(31.8%)和α-葡萄糖苷酶(43.8%)的抑制活性最低。总之,JSI对脂肪酶和碳水化合物水解酶表现出最高的清除活性和衰减作用。因此,JSI可能适合作为预防/管理食品相关疾病的治疗剂,而不是具有许多副作用的合成剂。
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引用次数: 1
Milk protein concentrate and reduced-calcium milk protein concentrate as natural emulsifiers in clean label high-protein ice cream manufacture 乳蛋白浓缩物和减钙乳蛋白浓缩物作为天然乳化剂在清洁标签高蛋白冰淇淋生产中的应用
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.009
Joseph Paglia , Chloe Fung , Chi Kong Yeung

Clean label food is a rising consumer trend in the food industry. Milk protein concentrate (MPC) and reduced-calcium milk protein concentrate (RCMPC) could serve as natural emulsifiers and increase the total protein content of ice cream products. The objectives of this study were to determine and compare the effects of MPC and RCMPC on ice cream composition, mix viscosity, storage stability, meltdown rate, and texture. A base formulation with 3% non-fat dry milk (NFDM) and no added emulsifiers was set as the control. Three levels of MPC or RCMPC (each powder containing 85% protein) at 1%, 2%, and 3% were incorporated by replacing equivalent amounts of NFDM and keeping other ingredients unchanged. All ice cream treatments were processed with a target overrun of 70% and hardened at −25 °C in a blast freezer. Additions of MPC or RCMPC at 1%, 2%, and 3% corresponded to increases in protein content of ice cream by 15%, 30%, and 45%, respectively. The viscosity of the ice-cream mix increased with increasing levels of MPC or RCMPC. In general, higher protein samples had slower meltdown rate and higher values of hardness and adhesiveness, but the trends were inconsistent. No shrinkage in volume was observed in any ice cream stored at −25 °C after 180 days. However, an additional storage stability study revealed that the control showed significant shrinkage after 60 days (−6.5% ± 1.5%), 90 days (−7.1% ± 1.8%), and 180 days (−7.9% ± 1.1%) in a typical household-style freezer at −13 °C. MPC at 1% also showed significant shrinkage after 180 days, while samples with RCMPC at any levels showed no shrinkage at all. Ice cream manufacturers may consider MPC and RCMPC natural alternatives to synthetic emulsifiers, with RCMPC being more effective than MPC in terms of ice cream storage stability.

清洁标签食品是食品行业中日益增长的消费趋势。浓缩乳蛋白(MPC)和降钙乳蛋白(RCMPC)可以作为天然乳化剂,提高冰淇淋产品的总蛋白质含量。本研究的目的是确定和比较MPC和RCMPC对冰淇淋成分、混合物粘度、储存稳定性、融化速率和质地的影响。将含有3%脱脂奶粉(NFDM)且不添加乳化剂的基础配方设定为对照。通过替换等量的NFDM并保持其他成分不变,掺入1%、2%和3%的三个水平的MPC或RCMPC(每个粉末含有85%的蛋白质)。所有冰淇淋处理都以70%的目标溢出率进行处理,并在−25°C的鼓风冷冻室中硬化。添加1%、2%和3%的MPC或RCMPC对应于冰淇淋的蛋白质含量分别增加15%、30%和45%。冰淇淋混合物的粘度随着MPC或RCMPC水平的增加而增加。一般来说,较高的蛋白质样品熔化速度较慢,硬度和粘附性较高,但趋势不一致。在−25°C下储存180天后,未观察到任何冰淇淋的体积收缩。然而,另一项储存稳定性研究显示,在−13°C的典型家用冰箱中,对照组在60天(−6.5%±1.5%)、90天(−7.1%±1.8%)和180天(−7.9%±1.1%)后出现显著收缩。1%的MPC在180天后也显示出显著的收缩,而任何水平的RCMPC样品都没有显示出任何收缩。冰淇淋制造商可能会考虑MPC和RCMPC作为合成乳化剂的天然替代品,在冰淇淋储存稳定性方面,RCMPC比MPC更有效。
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引用次数: 2
Complete genome sequence of Lactobacillus fermentum 9-4, a purine-degrading Lactobacillus probiotic isolated from Chinese fermented rice-flour noodles 从发酵米粉中分离的嘌呤降解益生乳杆菌发酵乳杆菌9-4的全基因组序列
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.008
Tiantian Gao, Lianghua Lu, Qingling Wu, Chenghua Wang

Lactobacillus fermentum 9-4 is a purine-degrading probiotic firstly isolated from traditional fermented rice-flour noodles with special characteristics of southern China's Guangxi Zhuang Autonomous Region. L. fermentum 9-4 could efficiently reduce the purine contents in foods with excellent purine-nucleoside assimilation and purine-metabolizing enzymatic activities. Crude cell lysate of L. fermentum 9-4 showed strong purine degradation abilities on nucleosides including inosine, guanosine, and purines including xanthine and uric acid, indicating a complete purine metabolic pathway to degrade purine substances to allantoin. In this study, the whole genome of L. fermentum 9-4 was sequenced. Its genome consisted of a 2 085 632 bp circular chromosome without plasmid. The GC content of circular chromosome was 51.25%, including 2 089 coding sequences (CDs), 15 rRNA operons and 59 tRNA coding genes. Genetic analysis showed that at least 5 protein coding genes were related to purine lowering ability. In addition, neither toxic virulence factor nor drug resistance gene was identified by searching the virulence factor database and drug resistance gene database, suggesting good safety of L. fermentum 9-4. The results showed that L. fermentum 9-4 has a good potential for the development and utilization as a purine-degrading probiotic for low-purine foods.

发酵乳杆菌9-4是从广西壮族自治区传统发酵米粉中首次分离得到的一种嘌呤降解益生菌。发酵乳杆菌9-4具有良好的嘌呤核苷同化和嘌呤代谢酶活性,可有效降低食品中的嘌呤含量。发酵乳杆菌9-4的粗细胞裂解物对核苷(包括肌苷、鸟苷)和嘌呤(包括黄嘌呤和尿酸)表现出较强的嘌呤降解能力,表明嘌呤物质降解为尿囊素的完整嘌呤代谢途径。本研究对发酵乳杆菌9-4的全基因组进行了测序。其基因组由2 085 632 bp的环状染色体组成,无质粒。环状染色体GC含量为51.25%,包括2089个编码序列、15个rRNA操纵子和59个tRNA编码基因。遗传分析表明,至少有5个蛋白质编码基因与嘌呤降低能力有关。此外,通过检索毒力因子数据库和耐药性基因数据库,未鉴定出毒性毒力因子和耐药性基因,表明发酵乳杆菌9-4具有良好的安全性。结果表明,发酵乳杆菌9-4作为一种用于低嘌呤食品的嘌呤降解益生菌具有良好的开发利用潜力。
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引用次数: 1
Determination of L-ergothioneine in food by UPLC-MS/MS method 超高效液相色谱-质谱联用法测定食品中l -麦角硫因
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-01 DOI: 10.1016/j.jfutfo.2022.12.007
Hongwen Zhai, Lei Sun, Sufang Fan, Jing Wang, Meirong Cao, Hongyu Ma, Qiang Li, Yan Zhang

L-Ergothioneine (L-EGT) possesses excellent antioxidant activity and has been used in the food, pharmaceuticals and cosmetics industries. In this study, a new efficient and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantitative determination of L-EGT in food. The sample was extracted with methanol-water (70:30, V/V), separated by hydrophilic interaction liquid chromatography (HILIC) and detected by triple-quadrupole mass spectrometry. Validation studies were carried out on different product and the limit of quantitation was 20 μg/kg (milk, alcohol-free beverages, dairy products) and 40 µg/kg (cereal bars, chocolate). Excellent linearity (correlation coefficient (R2) ≥ 0.999) was achieved for L-EGT quantification in the range of 5–200 ng/mL. The recoveries of the method (83.7%−107.5%) and the relative standard deviation (RSD, 0.88%−6.84% (n = 6)) meet the performance criteria required for the determination of L-EGT in food. Finally, the applicability of the method was tested by analysing actual samples. In general, the method developed is simple, reliable, accurate, and stable and could be useful for routine analyses of L-EGT in food.

L-麦角硫酮(L-EGT)具有良好的抗氧化活性,已被应用于食品、制药和化妆品行业。本研究建立了一种新的高效、灵敏的超高效液相色谱-串联质谱法(UPLC-MS/MS)测定食品中L-EGT的方法。样品用甲醇-水(70:30,V/V)萃取,用亲水相互作用液相色谱法(HILIC)分离,用三重四极杆质谱法检测。对不同的产品进行了验证研究,定量限为20μg/kg(牛奶、无酒精饮料、乳制品)和40μg/kg(谷物棒、巧克力)。L-EGT定量在5–200 ng/mL范围内实现了良好的线性(相关系数(R2)≥0.999)。方法的回收率为83.7%−107.5%,相对标准偏差为0.88%−6.84%(n = 6) )符合测定食品中L-EGT所需的性能标准。最后,通过对实际样品的分析,验证了该方法的适用性。总的来说,所开发的方法简单、可靠、准确、稳定,可用于食品中L-EGT的常规分析。
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Journal of Future Foods
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