Pharmacological Research - Natural Products最新文献

{"title":"Neuroprotective potential of ginger-derived α-santalol and β-eudesmol in Parkinson’s disease through network analysis","authors":"Rapuru Rushendran ,&nbsp;Pavithra Lakshmi Narayanan ,&nbsp;M. Jeevan Kumar ,&nbsp;J. Manoj, Manoah Mathew ,&nbsp;K. Gayathiri ,&nbsp;Chitra Vellapandian","doi":"10.1016/j.prenap.2026.100531","DOIUrl":"10.1016/j.prenap.2026.100531","url":null,"abstract":"<div><h3>Background</h3><div>Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by dopaminergic neuronal loss driven by oxidative stress, neuroinflammation, and protein misfolding. Natural products like ginger (<em>Zingiber officinale</em>) contain neuroprotective sesquiterpenes that may modulate these processes. This study aimed to evaluate the neuroprotective potential of ginger extract and its key bioactive compounds, α-santalol and β-eudesmol, using integrated computational approaches.</div></div><div><h3>Methods</h3><div>Ginger extract was profiled using GC-MS and LC-MS to identify major constituents. Network pharmacology tools (STRING, Metascape) were used to identify PD-related targets and construct protein-protein interaction networks. Pharmacokinetic and ADMET properties of α-santalol and β-eudesmol were predicted using SwissADME and pkCSM. Molecular docking was performed against PD-associated targets, including LRRK2, to explore potential binding interactions.</div></div><div><h3>Results</h3><div>Chemical profiling identified major bioactive compounds, including sesquiterpenes like α-santalol and β-eudesmol, with significant neuroprotective properties. Pharmacokinetic analysis revealed favorable BBB permeability, intestinal absorption, and low toxicity. Molecular docking demonstrated strong interactions with PD-related targets, particularly LRRK2, with binding energy values indicating effective target engagement. Pathway enrichment analysis highlighted the involvement of key pathways including NF-κB and Nrf2/ARE in mitigating oxidative stress and neuroinflammation. Biomolecule-target-pathway (B-T-P) networks confirmed the therapeutic relevance of the identified compounds.</div></div><div><h3>Conclusion</h3><div>Using a fully computational framework, this study identifies α-santalol and β-eudesmol as promising natural molecules with potential relevance to PD-associated pathways. These results are hypothesis-generating, and future biological validation through neuronal survival assays, oxidative-stress and inflammation models, and LRRK2-related functional studies is warranted.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100531"},"PeriodicalIF":0.0,"publicationDate":"2026-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of gamma radiation on physical properties, chemical composition and antibacterial activity of the essential oil from Eucalyptus pellita F. Muell.","authors":"Yelina González Pérez ,&nbsp;Ivan García-Fornaris ,&nbsp;Daniel Milian Pérez ,&nbsp;Abel Gámez Rodríguez ,&nbsp;Raquel Milani ,&nbsp;Antonio Celso Dantas Antonino ,&nbsp;Yaicel Ge Proenza","doi":"10.1016/j.prenap.2026.100534","DOIUrl":"10.1016/j.prenap.2026.100534","url":null,"abstract":"<div><div>The essential oils (EOs) from <em>Eucalyptus</em> species have high contents of oxygenated monoterpenes, demonstrated important antibacterial properties and are widely used in the pharmaceutical, cosmetic, and food industries. However, the stability and efficacy of EOs can be affected by environmental factors, such as exposure to radiation. The present study investigates the impact of gamma radiation on the chemical composition, physical properties and antibacterial activity of the essential oil (EO) from the leaves of <em>Eucalyptus pellita</em> F. Muell. The EO was extracted by hydro-distillation and analyzed by gas chromatography coupled mass spectrometry before and after exposure to 5–25 kGy of gamma radiation. The antibacterial activity of the EO was evaluated against two Gram-negative (G–), and two Gram-positive (G+) bacterial strains by the disk diffusion method. The gamma irradiation at the tested doses induced significant changes in the EO: reduction in monoterpenes such as eucalyptol and α-pinene, increase in sesquiterpenes, appearance of oxygenated compounds derived from radiolysis, and a notable increase in the density and refraction index. These modifications are further reflected in the antibacterial efficacy of the EO. The diameter of inhibition of the EO against the G– bacterial strains expands from average 9.9 to 13.2 ± 0.6 mm, and decreases from average 22.0 to 18.3 ± 0.8 mm against the G+ strains. These findings suggest that gamma radiation may be used to handle composition and antimicrobial properties of EOs for potential applications.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100534"},"PeriodicalIF":0.0,"publicationDate":"2026-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological and Computational Exploration of Ulva flexuosa: Antioxidant, Anti-inflammatory, Antidiabetic, Antipyretic, and Cytotoxic Insights for Drug Discovery","authors":"Md. Jahirul Islam Mamun ,&nbsp;Md. Hossain Rasel ,&nbsp;Md. Tanvir Chowdhury ,&nbsp;Md. Mahmudul Hasan ,&nbsp;Miton Chowdhury ,&nbsp;Thamjeed Mohaimeen ,&nbsp;Md Anower Kader ,&nbsp;Mohammad Wahiduzzaman ,&nbsp;Mohammad Forkanul Hamid ,&nbsp;S. M. Moazzem Hossen","doi":"10.1016/j.prenap.2026.100533","DOIUrl":"10.1016/j.prenap.2026.100533","url":null,"abstract":"<div><div>Over the past decade, <em>Ulva flexuosa</em>, a green tide seaweed, has proliferated in China's Yellow Sea. This study evaluated the pharmacological potential of its acetone extract (AEUF) through <em>in vitro</em>, <em>in vivo</em>, and computational methods. AEUF exhibited moderate to significant antioxidant activity with IC₅₀ values of 194.53 µg/mL (DPPH) and 50.78 µg/mL (ABTS). It showed notable anti-inflammatory effects in both HRBC membrane stabilization and carrageenan-induced paw edema models. For antidiabetic assessment, AEUF demonstrated strong α-amylase inhibition (IC₅₀ = 65.9 µg/mL) and significant hypoglycemic activity (p &lt; 0.001) in the oral glucose tolerance test (OGTT). Cytotoxicity evaluation via brine shrimp lethality assay revealed mild toxicity (LC₅₀ = 407.24 µg/mL), indicating a favorable safety profile. Molecular docking, PASS prediction, and ADME/T analyses supported the experimental results, highlighting the bioactive compounds’ binding affinity and drug-like properties. Overall, AEUF displays promising antioxidant, anti-inflammatory, antidiabetic, and hypoglycemic activities, with low cytotoxicity. These findings suggest that <em>U. flexuosa</em> is a valuable natural resource with significant pharmacological potential, particularly for managing oxidative stress, inflammation, and diabetes. Further studies are warranted to isolate active constituents and explore their mechanisms of action. This work underscores the importance of underutilized marine algae as a source of novel therapeutic agents.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100533"},"PeriodicalIF":0.0,"publicationDate":"2026-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial properties of leaf extracts of Coldenia procumbens L. and determination of phytochemical constituents using FT-IR and GC-MS","authors":"Chandrasekaran Yuvasri ,&nbsp;Devarajan Dhanavel ,&nbsp;Venugopalan Venkatesalu ,&nbsp;Mannathusamy Gopalakrishanan","doi":"10.1016/j.prenap.2026.100530","DOIUrl":"10.1016/j.prenap.2026.100530","url":null,"abstract":"<div><div>Herbal plants have been investigated for their potential in therapeutic drugs, food additives, agrochemicals, industrial chemicals, and various other applications. A significant number of pharmaceutical companies are allocating substantial resources to develop cost-effective natural medications derived from plant extracts. <em>Coldenia procumbens</em> L. is a prostrate herb characterised by small, alternating leaves. It is found throughout India, thriving in various environments from barren lands to paddy fields, and is commonly regarded as a weed species. This study aimed to assess the qualitative phytochemical constituents, characterise the phytocompounds using FT-IR and GC-MS, and evaluate the antibacterial potential of <em>C. procumbens</em>. The specimen was gathered from natural habitats in Kurinjipadi village, located in the Cuddalore district of Tamil Nadu, India. The extract underwent preliminary phytochemical screening to identify various phytochemical constituents, following the methodology outlined by Harborne (1998). The functional groups were identified using FT-IR, and the compounds were profiled through GC-MS analysis. The evaluation of antibacterial activities of crude extracts was conducted utilising the agar well diffusion method across different concentration levels of 25, 50, 75 and 100 µg/ml. Total of 14 notable compounds were detected via GC-MS analysis from three separate extracts. The most significant zone of inhibition noted in the antibacterial assessment was for <em>S. aureus,</em> measuring 28.91 ± 0.53 mm. This was succeeded by <em>E. faecalis</em> at 15.45 ± 0.46 mm and <em>E. coli</em> at 18.44 ± 0.38 mm, utilising petroleum ether, ethyl acetate, and aqueous extracts respectively. The findings highlight the potential of these plant-derived extracts as sources of natural antibacterial agents. The findings highlight the potential of these plant-derived extracts as effective natural antibacterial agents capable of strongly inhibiting pathogenic bacteria.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100530"},"PeriodicalIF":0.0,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal variation in phytochemical composition, quantitative analysis, and antioxidant activity of bulb extract from Urginea indica (Roxb.) Kunth","authors":"Uday Sahu ,&nbsp;Shriram Kunjam","doi":"10.1016/j.prenap.2026.100529","DOIUrl":"10.1016/j.prenap.2026.100529","url":null,"abstract":"<div><h3>Background</h3><div><em>Urginea indica</em> (Roxb.) Kunth is an important medicinal geophyte that has been traditionally used to treat a wide range of ailments, particularly cardiac and respiratory disorders. Its therapeutic value is largely attributed to the rich diversity of bioactive secondary metabolites present in the plant.</div></div><div><h3>Aim</h3><div>The aim of this study is to investigate the seasonal effect in the phytochemical constituents of the bulb of <em>Urginea indica</em> (Roxb.) Kunth, both qualitatively and quantitatively, as well as the antioxidant activity of the methanolic plant extract.</div></div><div><h3>Materials and methods</h3><div>In this study, bulb samples were collected across three distinct seasons (rainy, winter, and summer) and then extracted using aqueous, acetone, and methanol solvents through the Soxhlet apparatus. The antioxidant activity was analyzed by the DPPH assay.</div></div><div><h3>Results</h3><div>Quantitative analysis revealed that phenolic (3.799 mg GAE/g) and flavonoid (0.922 mg QE/g) contents were highest in winter, followed by summer (1.550 mg GAE/g and 0.800 mg QE/g, respectively), and their lowest concentrations in the rainy season (0.547 mg GAE/g and 0.405 mg QE/g). On the other hand, steroid (7.6 µg/g) and alkaloid (43.94 %) content were highest in the rainy season, reflecting their role in plant defense and growth. Antioxidant activity, determined by the DPPH assay, was concentration-dependent, scavenging activity with the methanolic extract having an IC₅₀ value of 584.172 µg/ml, although less potent than ascorbic acid (IC₅₀ = 36.961 µg/ml). The observed activity supports the potential medicinal relevance of <em>U. indica</em> as a natural source of bioactive compounds.</div></div><div><h3>Conclusion</h3><div>This research highlights the seasonal and solvent-dependent variation in the distribution of bioactive compounds in <em>U. indica</em> bulbs, indicating their potent antioxidant activity and potential medicinal value.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100529"},"PeriodicalIF":0.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of gallic acid and diosgenin in metabolic dysfunction-associated steatotic liver disease (MASLD) and non-alcoholic fatty liver disease (NAFLD): A comprehensive review","authors":"Trilochan Satapathy ,&nbsp;Mansi Verma ,&nbsp;Poonam Sahu ,&nbsp;Anjali Minj","doi":"10.1016/j.prenap.2026.100525","DOIUrl":"10.1016/j.prenap.2026.100525","url":null,"abstract":"<div><h3>Background</h3><div>Liver diseases remain a major global health concern, highlighting the need for safe and effective therapeutic alternatives. Plant-derived bioactives such as gallic acid and diosgenin have gained prominence due to their strong antioxidant, anti-inflammatory, and antifibrotic properties. Gallic acid, a phenolic compound found in fruits and herbs, and diosgenin, a steroidal sapogenin from Dioscorea species, support liver health by reducing oxidative stress, regulating inflammatory markers, restoring hepatic enzymes, and enhancing detoxification pathways including UGTs, GST, and CYP450 enzymes.</div></div><div><h3>Methodology</h3><div>A comprehensive literature search was performed across PubMed, Scopus, Web of Science, Science Direct, and Google Scholar for studies published between January 2000 and October 2024. Keywords used included “gallic acid,” “diosgenin,” “hepatoprotective,” “NAFLD,” “ALD,” “fibrosis,” “cirrhosis,” “DILI,” “oxidative stress,” “inflammation,” “TGF-β/Smad,” “PPAR-α,” “AMPK,” and detoxification-related terms. The search identified 1246 articles, with 934 remaining after duplicate removal. After screening and full-text evaluation of 186 articles based on relevance, mechanistic insights, and quality criteria, 112 studies were included in this review.</div></div><div><h3>Scope and approach</h3><div>This review examines the hepatoprotective effects of gallic acid and diosgenin across liver conditions such as ALD, NAFLD, DILI, fibrosis, and cirrhosis. It highlights their antioxidant, anti-inflammatory, antifibrotic, lipid-regulating, and detoxification-enhancing activities, focusing on key pathways including PPAR-α, AMPK, and TGF-β/Smad. Challenges such as low bioavailability and limited clinical evidence are also addressed.</div></div><div><h3>Key findings and conclusions</h3><div>Gallic acid and diosgenin demonstrate significant hepatoprotective potential, offering effects comparable to standard therapies with superior safety profiles. However, their clinical application is limited by poor solubility, low bioavailability, and inconsistent formulations. Enhancing pharmacokinetics, developing advanced delivery systems, and conducting robust clinical trials are crucial next steps. Overall, both compounds emerge as promising natural candidates for preventive and integrative liver therapy.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100525"},"PeriodicalIF":0.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive physicochemical, spectroscopic, and biological evaluation of Rosmarinus officinalis essential oil: From GC-MS profiling to ADMET prediction","authors":"Mustapha Mouzaki ,&nbsp;Khadija El Ouardy ,&nbsp;Soufiane El Megdar ,&nbsp;Zakaria benchama ,&nbsp;Fatima Hamadi ,&nbsp;Abdelaziz Elamrani ,&nbsp;Zohra Lemkhente ,&nbsp;Youssef Mir","doi":"10.1016/j.prenap.2026.100526","DOIUrl":"10.1016/j.prenap.2026.100526","url":null,"abstract":"<div><div>The essential oil (EO) of <em>Rosmarinus officinalis</em> collected from Ouarzazate, Morocco, showed significant antibacterial, antioxidant and antibiofilm activities. A comprehensive physicochemical characterization identified the major bioactive compounds responsible for these effects. The EO was obtained through hydrodistillation, and the chemical composition of the extracted EO was analysed using gas chromatography-mass spectrometry (GC-MS). Physicochemical characterization included Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) spectroscopy, UV-Vis spectrophotometry, optical rotation (°), refractive index, solubility, and density measurements. The major EO constituents were identified as α-pinene, eucalyptol, and camphor, with physicochemical properties such as an optical rotation of + 6.5°, a refractive index of 1.47, and a density of 0.792. Antioxidant activity, assessed using the DPPH radical scavenging assay, showed 52 % inhibition, while antibacterial tests demonstrated strong inhibitory activity against Gram-positive and Gram-negative bacteria growth, with inhibition zones ranging from 7.33 mm (<em>P. aeruginosa</em>) to 20.83 mm (<em>K. pneumoniae</em>), while MICs varied from 0.20 µL/ml (<em>B. clausii</em>) to 6.45 µL/ml (<em>Enterococcus spp</em>. and <em>MDR E. coli</em>). Additionally significant antibiofilm activity was observed with highest against <em>S. aureus</em> (96 % inhibition at MIC) and lowest against <em>E. coli</em> (46 % at MIC/4). Molecular docking and ADMET in silico studies were consistent with previous in vitro findings, revealing favourable binding affinities and pharmacokinetic properties. This study confirms rosemary EO's potential as an effective antioxidant, antibacterial, and antibiofilm agent. Therefore <em>R. officinalis</em> essential oil may serve as a promising natural therapeutic component for use in traditional medicine, aromatherapy, and future pharmaceutical formulations.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100526"},"PeriodicalIF":0.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose dependent behavioral effects of agmatine in rats","authors":"Hira Rafi ,&nbsp;Hamna Rafiq ,&nbsp;Muhammad Farhan","doi":"10.1016/j.prenap.2026.100528","DOIUrl":"10.1016/j.prenap.2026.100528","url":null,"abstract":"<div><h3>Background</h3><div>Agmatine, a decarboxylated metabolite of <span>L</span>-arginine, exhibits neuroprotective, antidepressant, and anxiolytic properties through its modulation of NMDA receptors, monoamine pathways, and antioxidant defenses. Although its therapeutic potential is well recognized, its dose-dependent behavioral and biochemical effects remain insufficiently characterized.</div></div><div><h3>Objective</h3><div>This study investigated the chronic, dose-dependent effects of agmatine on locomotor activity, anxiety-like and depressive-like behaviors, cognitive performance, and oxidative stress markers in rats.</div></div><div><h3>Methods</h3><div>albino Wistar rats (n = 24) received oral agmatine at 50, 100, or 200 mg/kg for 28 days, while controls received water. Behavioral assessments included the open field test, forced swim test, elevated plus maze, light/dark transition test, and Morri’s water maze. Oxidative stress markers (MDA, SOD, CAT, GSH, GPx) were quantified from brain homogenates. Statistical analysis was performed using one-way ANOVA (p &lt; 0.05).</div></div><div><h3>Results</h3><div>Agmatine produced significant dose-dependent improvements in locomotion and exploration, with the most pronounced effects at 100 and 200 mg/kg. Antidepressant-like behavior was evident through increased struggling in the forced swim test (p &lt; 0.01), while anxiolytic effects were observed across all doses in both the elevated plus maze and light/dark transition tasks (p &lt; 0.01). Cognitive function improved significantly, as shown by reduced escape latencies during training, acquisition, and retention phases of the Morris water maze (p &lt; 0.01). Biochemically, agmatine reduced lipid peroxidation and elevated enzymatic antioxidant activity in a dose-dependent manner. 100 mg/kg dose consistently produce the strongest behavioral and antioxidant responses.</div></div><div><h3>Conclusion</h3><div>Chronic agmatine administration exerts robust dose-dependent behavioral and neuroprotective effects, with 100 mg/kg identified as the optimal effective dose. These findings support agmatine as a safe candidate for therapeutic applications targeting anxiety, depression, cognitive impairment, and oxidative stress.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100528"},"PeriodicalIF":0.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the therapeutic potential of Firmiana colorata bark extract: GC-MS profiling, pharmacological studies, and protein-ligand interaction analysis for pain, fever, and inflammation","authors":"Saurav Singha,&nbsp;Md. Ekramul Haque Ekram ,&nbsp;Md. Liakot Ali ,&nbsp;Md. Mahmudul Hasan ,&nbsp;Md. Tanveer Ahsan","doi":"10.1016/j.prenap.2026.100523","DOIUrl":"10.1016/j.prenap.2026.100523","url":null,"abstract":"<div><h3>Background</h3><div><em>Firmiana colorata</em>, also referred to as \"Mula,\" is a traditional medicinal plant in China and the Indian subcontinent.</div></div><div><h3>Objective</h3><div>This study aimed to evaluate the antinociceptive, antipyretic, and anti-inflammatory activities of the methanol extract of <em>Firmiana colorata’s</em> bark (MEFCB) in rodent models.</div></div><div><h3>Methods</h3><div>Qualitative phytochemical analysis and gas chromatography–mass spectrometry (GC–MS) were performed on MEFCB. In animal models at doses of 200 mg/kg and 400 mg/kg, the antinociceptive activity was evaluated using the formalin-induced paw licking test and the acetic acid-induced writhing response. Whereas brewer's yeast-induced pyrexia and carrageenan-induced paw edema were used to assess the antipyretic and anti-inflammatory properties.</div></div><div><h3>Results</h3><div>Qualitative phytochemical screening of the extract revealed the presence of alkaloids, tannins, glycosides, and flavonoids. The bark extract's GC-MS profile revealed 22 phytochemicals. The phytochemicals were assessed using <em>in silico</em> pass prediction, ADME/T, and molecular docking. The extracts' antinociceptive effects demonstrated a decrease in the writhing and paw-licking methods. Pyrexia was substantially decreased in antipyretic investigations. Besides, a significant reduction of paw edema (P &lt; 0.001) was observed in the anti-inflammatory test. The top compound 1,2-benzenediol, o (3-methylbut-2-enoyl)-o′-(pivaloyl)-6 demonstrated strong binding affinities, with binding scores of −8 and −7.9 (kcal/mol) against the crucial drug target proteins, cyclooxygenase-1 (PDB ID: 2OYE) and cyclooxygenase-2 (PDB ID: 6COX).</div></div><div><h3>Conclusion</h3><div><em>Firmiana colorata</em> can be a valuable source of bioactive compounds for the treatment of pain, fever, and inflammation. However, more researches are required to explore its full therapeutic potential.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100523"},"PeriodicalIF":0.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the phytochemical contents and therapeutic potential of Litsea monopetala leaves: Hypoglycemic and antibacterial activities","authors":"Zubair Khalid Labu ,&nbsp;Samira Karim ,&nbsp;Rahima Akter ,&nbsp;Sarder Arifuzzaman ,&nbsp;Md. Hasibul Hassan ,&nbsp;Md. Nahid Hasan ,&nbsp;Atiqur Rahman ,&nbsp;Md. Tarekur Rahman ,&nbsp;Md. Ataur Rahman ,&nbsp;Asma Akhtar","doi":"10.1016/j.prenap.2026.100527","DOIUrl":"10.1016/j.prenap.2026.100527","url":null,"abstract":"<div><div><em>Litsea monopetala</em> (LM), and bark are applied externally to treat swelling, bruises, skin infections, and inflammatory conditions. Occasionally, leave decoctions are used as topical washes or mild teas for digestive ailments and fever; however, internal use remains limited and guided by local knowledge. This study aimed to scientifically validate these traditional uses by assessing the hypoglycemic and antibacterial properties of LM leaves. Methanolic extracts were prepared via cold extraction and analyzed through standard phytochemical techniques and High-Performance Liquid Chromatography, revealing a high concentration of phenolic and flavonoid compounds. Oral Glucose Tolerance Tests in mice showed that both 250 mg/kg and 500 mg/kg doses significantly reduced blood glucose levels, with the higher dose yielding effects comparable to glibenclamide. In alloxan-induced diabetic mice, blood glucose levels dropped from 240.90 ± 6.16–145.17 ± 4.19 mg/dL by day 7 (p &lt; 0.01). In normal mice, the 500 mg/kg dose also caused a significant reduction (p &lt; 0.01). Antibacterial activity, evaluated using the disc diffusion method, revealed inhibition zones of 10–21 mm at a 400-μg dose, with carbon tetrachloride soluble fraction, chloroform soluble fraction, and ethyl acetate soluble fraction fractions demonstrating significantly stronger effects (p &lt; 0.05). These findings support the traditional use of LM and suggest potential for its development into hypoglycemic and antimicrobial agents. The results support the traditional use of LM leaves, highlighting strong hypoglycemic and antibacterial potential. These effects are likely linked to rich phenolic and flavonoid content, indicating promise for future therapeutic applications.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"10 ","pages":"Article 100527"},"PeriodicalIF":0.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}