{"title":"A NOTE ON THE INTERACTION OF PITUITRIN AND INSULIN","authors":"M. Umezu, Torai Shimamura","doi":"10.1292/JVMS1922.7.1","DOIUrl":"https://doi.org/10.1292/JVMS1922.7.1","url":null,"abstract":"","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130916877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"UNTERSUCHUNGEN ÜBER DIE POCKEN DER SCHWEINE IN MANDSCHUREI MIT BESONDERER BERÜCKSICHTIGUNG DER KLINISCHEN BEFUND","authors":"Masaichi Yoshikawa","doi":"10.1292/JVMS1922.9.16","DOIUrl":"https://doi.org/10.1292/JVMS1922.9.16","url":null,"abstract":"","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127626593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TERMINAL DISTRIBUTION OF THE RADIAL NERVE IN THE HORSE","authors":"S. Matsuba, Iwao Hiroye","doi":"10.1292/JVMS1922.7.257","DOIUrl":"https://doi.org/10.1292/JVMS1922.7.257","url":null,"abstract":"","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131675247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
On February 20, 1935, we found a large malignant tumor in the liver of a foetus of a mare which has aborted in about the ninth month of pregnancy, and the tumor was diagnosed as primary carcinoma originated from epithelium of the bile-duct.The outlines of the pathological changes of the foetus were as follows.-The foetus is emaciated, but the lower part of the neck and the abdomen are enormously distended, and the circumference of the latter attains about 97 cm.The visible mucous membranes are slightly yellowish in colour.The abdominal cavity contains about 15000 c.c. of turbid brownish red fluid. The abdominal and thoracic cavities communicate by means of a rupture in the lower part of the diaphragm.The distended part of the neck harbors a cavity which contains about 1000 c.c. of turbid brownish-red fluid, and the cavity communicates with the thoracic cavity by a fissure at the anterior aperture of the thorax.The liver is markedly swollen and adherent to the right kidney and to the diaphragma. It weighed 3190 gm.. The right lobe includes a tumor about the size of child's head and is some-what prominent on the surface. On transverse section of this lobe, the tumor is irregularly round, 15 cm. in diameter, and shows pus like discharge, in which some small, yellowish granules can be found.The tumor is very soft, of spongy consistency, granulated and yellowish-white in colonr. The left and the middle lobes are also swollen, congested, dark greenish-yellow in colour, and haemorrhagic patches are scattered on their surface. The section of these lobes shows fine network, dark greenish in colour, and the same coloured fluid can be pressed out.Microscopic appearance.The essential elements of the tumor are epithelial cells with large deeply staining nuclei, very scanty cytoplasma, and the cells resemble more or less the epithelium of bile-ducts.On the periphery of the tumor, the tumorcelles are arranged in alveoli of irregular shape and size, and the meshwork of the stroma is abundant and composed of dense fibrous tissue.At the centre of the tumor, the connective tissue is meagre in amount and there are many granular substances which are deeply stained by haematoxylin, and necrotic or haemorrhagic areas are also observed.The remaining part of the liver is in general congested, inter-stitial tissue is developed abundantly around each lobule (mono-lobular cirrhosis) and the cells of the liver parenchyma are small and irregular in shape.Namely, the tumor is very malignant and embryonic in type, and it may be considerd as a carcinoma originated from the epithelium of the bile-duct.
{"title":"A primary carcinoma in the liver of a foetus","authors":"H. Oguni, T. Ishida","doi":"10.1292/jvms1922.15.214","DOIUrl":"https://doi.org/10.1292/jvms1922.15.214","url":null,"abstract":"On February 20, 1935, we found a large malignant tumor in the liver of a foetus of a mare which has aborted in about the ninth month of pregnancy, and the tumor was diagnosed as primary carcinoma originated from epithelium of the bile-duct.The outlines of the pathological changes of the foetus were as follows.-The foetus is emaciated, but the lower part of the neck and the abdomen are enormously distended, and the circumference of the latter attains about 97 cm.The visible mucous membranes are slightly yellowish in colour.The abdominal cavity contains about 15000 c.c. of turbid brownish red fluid. The abdominal and thoracic cavities communicate by means of a rupture in the lower part of the diaphragm.The distended part of the neck harbors a cavity which contains about 1000 c.c. of turbid brownish-red fluid, and the cavity communicates with the thoracic cavity by a fissure at the anterior aperture of the thorax.The liver is markedly swollen and adherent to the right kidney and to the diaphragma. It weighed 3190 gm.. The right lobe includes a tumor about the size of child's head and is some-what prominent on the surface. On transverse section of this lobe, the tumor is irregularly round, 15 cm. in diameter, and shows pus like discharge, in which some small, yellowish granules can be found.The tumor is very soft, of spongy consistency, granulated and yellowish-white in colonr. The left and the middle lobes are also swollen, congested, dark greenish-yellow in colour, and haemorrhagic patches are scattered on their surface. The section of these lobes shows fine network, dark greenish in colour, and the same coloured fluid can be pressed out.Microscopic appearance.The essential elements of the tumor are epithelial cells with large deeply staining nuclei, very scanty cytoplasma, and the cells resemble more or less the epithelium of bile-ducts.On the periphery of the tumor, the tumorcelles are arranged in alveoli of irregular shape and size, and the meshwork of the stroma is abundant and composed of dense fibrous tissue.At the centre of the tumor, the connective tissue is meagre in amount and there are many granular substances which are deeply stained by haematoxylin, and necrotic or haemorrhagic areas are also observed.The remaining part of the liver is in general congested, inter-stitial tissue is developed abundantly around each lobule (mono-lobular cirrhosis) and the cells of the liver parenchyma are small and irregular in shape.Namely, the tumor is very malignant and embryonic in type, and it may be considerd as a carcinoma originated from the epithelium of the bile-duct.","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"121 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131724937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"UEBER KOMPLEMENTBINDUNGSVERSUCH BEI DEN SEREN PULLORUMINFIZIERTER HÜHNER, IM ZUSAMMENHANG MIT DER PRÄZIPITATION","authors":"T. Konno, K. Zaizen","doi":"10.1292/JVMS1922.12.129","DOIUrl":"https://doi.org/10.1292/JVMS1922.12.129","url":null,"abstract":"","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129018027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anti-Fluorescent Effect of the Growth of Hemolytic Streptococci on that of Organisms belonging to the Pasteurella Group. II","authors":"Seizo Watanabe","doi":"10.1292/JVMS1922.16.297","DOIUrl":"https://doi.org/10.1292/JVMS1922.16.297","url":null,"abstract":"","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125422357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1. Als Erreger der Rinderpiroplasmose in diesem Gebiete sind 2 Arten des Pirplasmas, namlich eine grosse, Bigeminum ahnliche und eine kline, Mutans ahnliche, zu erkennen.2. Die Mischinfektion dieser beiden Arten kann etwa die eine Halfte der infizierten Rinder mit charakteristischen Symptomen mindestens ohne besondere Bedingung befallen, wahrend die Reininfektion der kleinen erst mit besonderer Bedingung (Abweidung, Gebaren, Transportion usw.) befallen kann. Die durch diese Mischund Reininfektion verursachten Krankheiten machen fast keinen Unterschied.3. Unten dem Mikroskope zeigt sich die kleine Art immer im periphren Blute der infizierten Rinder, die grosse dagegen nur selten. Fur die Bestimmung der Grosspiroplasmeninfektion ist somit der Uebertragungsversuch die sicherste Methode.4. Die Krankheit oder die Mischinfektion der beiden zu bestehen gibt den Rindern eine gewisse Immunitat, die Reininfektion der kleinen selbst gibt aber keine.5. Dir kunstliche Michinfektion mit besonderer Berucksichtigung ist als Schutzimpfung gegen diese Krankheit in diesem verseuchten Gebiete zu brauchen.6. Die charkteristischen Symptome dieser Krankheit sind folgende: Mattigkeit, Anamie, Gelbscucht, Hamoglobinurie und Abnormalerythrozyten in Peripherblute. Von allem spielt die letzte die Hauptrolle, diese Krankheit zu diagnosieren.7. Die wichtigste Sektionsbefunde sind: Milz: Vergrosserung infolge Ueberfullung mit Blut, ausstrichbare Pulpa (haufig weich zerfliessend in subakuten Fallen). Leber: Stark Gallenstauung, parenchymatose Degeneration. Niere: Parenchymatose Degeneration und Hamosiderose (besonders in den gewundenen Harnkanalchen. Lunge: Oedem (mitunter). Ausserdem in fast allen Organen Oedem, Gelbsucht und Hamosiderose.8. Trypanblau (Cassella, Hochst) gibt keine besondere (chemotherapeutische) Wirkung gegen diese Piroplasmose.Der Autor hat viele Mitteilungen uber Rinderpiroplasmose studiert und auch die Blut- und Organausstrichpraparate von Piroplasma bigemium, mutuns und parvum, die Sir A. Theiler, Herrn Prof. Dr. Ch. Kohanawa gegeben hatte, und die Blutausstrichpraparate von P. bovis, die P. Iwanoff (Kharkov) dem Autor freundlich geschenkt hatte, genau untersucht. Von diesen Praparaten zeigen einige Abnormalerythozyten aufweisende Praparate ziele Kleinpiroplasmen (P. mutans), aber die Grosspiroplasmen (P. bigeminum oder P. bovis) nur gering oder gar nicht. Morphologisch ist das Grosspiroplasma in dieser Gegend dem Piroplasma bigeminum oder Piroplasma bovis und das Kleine dem Piroplasma mutans sehr ahnlich. Durch diese vergleichenden Untersuchungen wurde folgende Annahme vom Autor gewonnen: Es kann moglich sein, dass die Kleinpiroplasmen und die Grosspiroplasmen (ausschliesslich des P. parvum) die gemeinschaftlichen Erreger einer uber verschiedene Lander verbreiteten Piroplasmose sind, die in ihren Symptomen verschiedene Variationen zeigen, mit anderen Worten, es mussen die Rinderpiroplasmosen ausser dem Kustenfieber in verschiedenen Landern fur gleich geh
1. 这部分地区有两种普斯普拉马斯生物，很著名的是一个又大又多灰的水母和一个圆柱，也就是穆勒的嗅觉者。2这两种病毒的混合有时会使有一半受到感染的牛出现独特症状，然后至少没有一个特殊条件。这类杂交种和另外一种疾病给造成的影响很不一样。在显微镜下是小品种，被感染的牛的腹部出血。因此无法用最安全的方法来检测病毒感染牛群感染水牛这项研究给了牛一种免疫力，却没有为牛儿成功感染。5考虑在这种受污染地区提供专门针对该疾病提供的保护服务。6这种病的迷人症状有:泥质、无序性、黄疸、血红蛋白和周围血液中的不正常红细胞。确诊这种疾病时，最后一位主演是? 7最重要的化验结果是:脾脏:用血和拔出的脉状冷却(在亚原子里产生大量柔软碎屑)。肝:重肠胃衰竭。肾:尿道瘤引起衰竭和血肿(特别是在弯曲的尿道中)。肺:坦白地说。还必须在没有正式演出的时候正式练习崔氏蓝(Cassella，高点)没有对这皮质病症有什么特别的效果。这位作家出版了许多来文uber Rinderpiroplasmose大学同时也是血的Organausstrichpraparate Piroplasma bigemium mutuns和parvum博士教授f . a . Theiler爵士主Kohanawa政府曾和Blutausstrichpraparate p bovis, p Iwanoff (Kharkov)作者在友好送她,就调查.不过基本的情况却截然不同。在这些自称的幻像中，一些不正常的自称细胞有小像(p。形态学，这里几个地方的一种形体“智形”(智形)叫波维兹(bovis)，小体则跟穆坦的智形很像。受到这种比较研究的假设从作者赢了:有的可能现如今Kleinpiroplasmen和Grosspiroplasmen (p . parvum)的仅仅集体病原体的uber不同岁分发Piroplasmose是看其症状的不同变化,换句话说,它必须Rinderpiroplasmosen除了Kustenfieber在不同Landern给马上被.最后，我要感谢我的名誉老师K. ka拔剑教授他每时每刻都给我好好指点乌鲁德谢谢k教授允许我沐浴川松博士，黑泽博士，粉贺川博士谢谢您允许您使用这些疾病的原料
{"title":"UEBER RINDERPIROPLASMOSE IN HOKKAIDO (JAPAN)","authors":"K. Ogura","doi":"10.1292/JVMS1922.8.1","DOIUrl":"https://doi.org/10.1292/JVMS1922.8.1","url":null,"abstract":"1. Als Erreger der Rinderpiroplasmose in diesem Gebiete sind 2 Arten des Pirplasmas, namlich eine grosse, Bigeminum ahnliche und eine kline, Mutans ahnliche, zu erkennen.2. Die Mischinfektion dieser beiden Arten kann etwa die eine Halfte der infizierten Rinder mit charakteristischen Symptomen mindestens ohne besondere Bedingung befallen, wahrend die Reininfektion der kleinen erst mit besonderer Bedingung (Abweidung, Gebaren, Transportion usw.) befallen kann. Die durch diese Mischund Reininfektion verursachten Krankheiten machen fast keinen Unterschied.3. Unten dem Mikroskope zeigt sich die kleine Art immer im periphren Blute der infizierten Rinder, die grosse dagegen nur selten. Fur die Bestimmung der Grosspiroplasmeninfektion ist somit der Uebertragungsversuch die sicherste Methode.4. Die Krankheit oder die Mischinfektion der beiden zu bestehen gibt den Rindern eine gewisse Immunitat, die Reininfektion der kleinen selbst gibt aber keine.5. Dir kunstliche Michinfektion mit besonderer Berucksichtigung ist als Schutzimpfung gegen diese Krankheit in diesem verseuchten Gebiete zu brauchen.6. Die charkteristischen Symptome dieser Krankheit sind folgende: Mattigkeit, Anamie, Gelbscucht, Hamoglobinurie und Abnormalerythrozyten in Peripherblute. Von allem spielt die letzte die Hauptrolle, diese Krankheit zu diagnosieren.7. Die wichtigste Sektionsbefunde sind: Milz: Vergrosserung infolge Ueberfullung mit Blut, ausstrichbare Pulpa (haufig weich zerfliessend in subakuten Fallen). Leber: Stark Gallenstauung, parenchymatose Degeneration. Niere: Parenchymatose Degeneration und Hamosiderose (besonders in den gewundenen Harnkanalchen. Lunge: Oedem (mitunter). Ausserdem in fast allen Organen Oedem, Gelbsucht und Hamosiderose.8. Trypanblau (Cassella, Hochst) gibt keine besondere (chemotherapeutische) Wirkung gegen diese Piroplasmose.Der Autor hat viele Mitteilungen uber Rinderpiroplasmose studiert und auch die Blut- und Organausstrichpraparate von Piroplasma bigemium, mutuns und parvum, die Sir A. Theiler, Herrn Prof. Dr. Ch. Kohanawa gegeben hatte, und die Blutausstrichpraparate von P. bovis, die P. Iwanoff (Kharkov) dem Autor freundlich geschenkt hatte, genau untersucht. Von diesen Praparaten zeigen einige Abnormalerythozyten aufweisende Praparate ziele Kleinpiroplasmen (P. mutans), aber die Grosspiroplasmen (P. bigeminum oder P. bovis) nur gering oder gar nicht. Morphologisch ist das Grosspiroplasma in dieser Gegend dem Piroplasma bigeminum oder Piroplasma bovis und das Kleine dem Piroplasma mutans sehr ahnlich. Durch diese vergleichenden Untersuchungen wurde folgende Annahme vom Autor gewonnen: Es kann moglich sein, dass die Kleinpiroplasmen und die Grosspiroplasmen (ausschliesslich des P. parvum) die gemeinschaftlichen Erreger einer uber verschiedene Lander verbreiteten Piroplasmose sind, die in ihren Symptomen verschiedene Variationen zeigen, mit anderen Worten, es mussen die Rinderpiroplasmosen ausser dem Kustenfieber in verschiedenen Landern fur gleich geh","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125492041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It is well known that animals may be protected from tetanus infection by the previous injection of tetanus antitoxin and the immunity thus produced lasts, however, only for a short time (a few weeks). To our regret there has been no safe and practicable method of inoculation against tetanus yet, which confers a long-lasting immunity on animals treated.In the year 1917 Vallee and Bazy published a method of active immunization against tetanus. After demonstrating that injections of tetanus toxin attenuated by the addition of a iodine solution (iodine, 1; potassium iodide, 2; distilled water, 200) conferred a solid immunity on the rabbits treated, they treated 7 seriously wounded persons (who had received prophylactic injections of antitetanic serum) with the iodized tetanus toxin at the military hospital at Begin; the patients received 3 subcutaneous injections at intervals of 5 days, 1c.c. and 2c.c. of a mixture containing 2 parts of toxin and 1 part of iodine solution, and finally 5c.c. of a mixture of 3 parts toxin and 2 parts iodine solution. The French authors concluded that this method of injecting the iodized tetanus toxin is safe and confers a long-lasting immunity.The writer carried out a series of experiments to ascertain the effect of Vallee and Bazy's method. To obtain the tetanus toxin the tetanus bacillus was grown aerobically in "liver-piece broth" with the addition of 10-20 per cent. fresh blood (the blood of cattle, horse or sheep is equally usable), and the lethal dose of the toxin thus obtained is usually 0, 00001-0, 000005c.c. for a mouse of about 10 grams, 0, 0001-0, 00005c.c. for a guineapig of about 400 grams, and 0, 001c.c. for a rabbit of about 2000 grams. As the experimental animals were used a great number of guinea-pigs and rabbits, and a few sheep, goats and horses.The writer found that a mixture of tetanus toxin and iodine solution in proportion of 2:1, as proposed by Vallee and Bazy, is dangerous to guine-apigs and rabbits, the whole of the former (37) and a majority of the latter (7) developing tetanus, and mortality being about 60 and 43 per cent. respectively, while that in proportion of 1:1 is quite safe for both kinds of animals. Two injections of the mixture of 1:1 (1 and 2c.c.) and third injection of that of 2.5:2 (4, 5c.c.) conferred a solid immunity on guinea-pigs treated which resisted the control inocutation of a lethal dose of the toxin, with no reaction.It is much easier to immunize rabbits, one treatment being sufficient for this purpose, when used a dose of 2-4, 5c.c. of amixture of 1:1, 2, 5:2, 2:1, 5, 1, 5:1 or 2, 5:1, 5.In goats the immunity was produced by two injections of 2c.c. of 1:1 and 4-4, 5c.c. of 2, 5:2 or 2, 5:1, 5. Sheep and horses were also immunized by two injections of 5c.c. of 1:1 and 8c.c. of 2, 5:1, 5.The iodized toxin retained its prophylactic value for about 40 days after being mixed with the iodine solution.
{"title":"ON THE PROPHYLACTIC VALUE OF IODIZED TETANUS TOXIN","authors":"K. Okuda","doi":"10.1292/JVMS1922.2.65","DOIUrl":"https://doi.org/10.1292/JVMS1922.2.65","url":null,"abstract":"It is well known that animals may be protected from tetanus infection by the previous injection of tetanus antitoxin and the immunity thus produced lasts, however, only for a short time (a few weeks). To our regret there has been no safe and practicable method of inoculation against tetanus yet, which confers a long-lasting immunity on animals treated.In the year 1917 Vallee and Bazy published a method of active immunization against tetanus. After demonstrating that injections of tetanus toxin attenuated by the addition of a iodine solution (iodine, 1; potassium iodide, 2; distilled water, 200) conferred a solid immunity on the rabbits treated, they treated 7 seriously wounded persons (who had received prophylactic injections of antitetanic serum) with the iodized tetanus toxin at the military hospital at Begin; the patients received 3 subcutaneous injections at intervals of 5 days, 1c.c. and 2c.c. of a mixture containing 2 parts of toxin and 1 part of iodine solution, and finally 5c.c. of a mixture of 3 parts toxin and 2 parts iodine solution. The French authors concluded that this method of injecting the iodized tetanus toxin is safe and confers a long-lasting immunity.The writer carried out a series of experiments to ascertain the effect of Vallee and Bazy's method. To obtain the tetanus toxin the tetanus bacillus was grown aerobically in \"liver-piece broth\" with the addition of 10-20 per cent. fresh blood (the blood of cattle, horse or sheep is equally usable), and the lethal dose of the toxin thus obtained is usually 0, 00001-0, 000005c.c. for a mouse of about 10 grams, 0, 0001-0, 00005c.c. for a guineapig of about 400 grams, and 0, 001c.c. for a rabbit of about 2000 grams. As the experimental animals were used a great number of guinea-pigs and rabbits, and a few sheep, goats and horses.The writer found that a mixture of tetanus toxin and iodine solution in proportion of 2:1, as proposed by Vallee and Bazy, is dangerous to guine-apigs and rabbits, the whole of the former (37) and a majority of the latter (7) developing tetanus, and mortality being about 60 and 43 per cent. respectively, while that in proportion of 1:1 is quite safe for both kinds of animals. Two injections of the mixture of 1:1 (1 and 2c.c.) and third injection of that of 2.5:2 (4, 5c.c.) conferred a solid immunity on guinea-pigs treated which resisted the control inocutation of a lethal dose of the toxin, with no reaction.It is much easier to immunize rabbits, one treatment being sufficient for this purpose, when used a dose of 2-4, 5c.c. of amixture of 1:1, 2, 5:2, 2:1, 5, 1, 5:1 or 2, 5:1, 5.In goats the immunity was produced by two injections of 2c.c. of 1:1 and 4-4, 5c.c. of 2, 5:2 or 2, 5:1, 5. Sheep and horses were also immunized by two injections of 5c.c. of 1:1 and 8c.c. of 2, 5:1, 5.The iodized toxin retained its prophylactic value for about 40 days after being mixed with the iodine solution.","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123147228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
(1) Several methods of extraction of the active princple from the total pancreatic tissue are summarized. The authors recommend, to save time and alcohol, the use of sodium chloride or ammonium sulphate during the vacuum distillation of the alcoholic filtrate.(2) The effects of the proteoclastic action of pepsin and trypsin on the hormone were studied with mice as test animals. The results obtained corroborate those of Dudley and of Witzemann and Livshis. By peptic digestion insulin loses about one-fourth of its potency in thirty minutes, one hour being required for the total destruction. The tryptic action is completed in thirty minutes.(3) Insulin reaction in the experimental animals are described. In mice about one-hundredth rabbit unit insulin per ten grams of body weight is required to cause the hypoglycemic reactions with convulsions. This dose caused the typical reactions in 33 and 50% of two lots of fed mice in one hour. In other two lots of fasted animals the percentage of the insulin effect rose to 60 and 65%, where the comatose reaction only has predominated. The authors resort conveniently to the use of mice at least for the preliminary assay of insulin.Young albino rats of 20-50 grams of body weight, with dose of one-hundredth rabbit unit per ten grams of live weight, behave just same as in mice. The older animals over two hundred grams are with the same dose very resistent towards the hypoglycemic convulsions.The insulin convulsions and coma are believed not to occur in pigeon, though the blood sugar level may be easily lowered, with several rabbit units of insulin, to about 50-60% of the normal. The authors describe one rare case of the convulsive reaction in pigeon with two rabbit units dose. The bird convulsed several times during the course of ten hours. The lowest level of the blood sugar was found to be 0, 077 grams per 100c.c.In horse therhypoglycemic convulsions can be easily elicited with one half rabbit units per kilo of body weight. The convulsive blood sugar level was about 0, 050 grams per 100c.c.(4) The efficiency of various sugars in relieving the symptoms caused by insulin in albino rats was studied. The results obtained agree essentially with those of Noble and Macleod in rabbit and of Herring, Irvine and Macleod in mice. Put the quantity of glucose just required (0, 020-0, 025, grams per ten grams of live weight)=1, then mannose=2, maltose=3, fructose>3, galactose>3, and arabinose>4.(5) Insulin exerts no perceptible action on the course of avian beriberi, and vitamin B (oryzanin) has also no influence upon the insulin reactions.
{"title":"EXPERIMENTS WITH THE PANCREATIC HORMONE INSULIN","authors":"Torai Shimamura, R. Takahashi","doi":"10.1292/JVMS1922.4.69","DOIUrl":"https://doi.org/10.1292/JVMS1922.4.69","url":null,"abstract":"(1) Several methods of extraction of the active princple from the total pancreatic tissue are summarized. The authors recommend, to save time and alcohol, the use of sodium chloride or ammonium sulphate during the vacuum distillation of the alcoholic filtrate.(2) The effects of the proteoclastic action of pepsin and trypsin on the hormone were studied with mice as test animals. The results obtained corroborate those of Dudley and of Witzemann and Livshis. By peptic digestion insulin loses about one-fourth of its potency in thirty minutes, one hour being required for the total destruction. The tryptic action is completed in thirty minutes.(3) Insulin reaction in the experimental animals are described. In mice about one-hundredth rabbit unit insulin per ten grams of body weight is required to cause the hypoglycemic reactions with convulsions. This dose caused the typical reactions in 33 and 50% of two lots of fed mice in one hour. In other two lots of fasted animals the percentage of the insulin effect rose to 60 and 65%, where the comatose reaction only has predominated. The authors resort conveniently to the use of mice at least for the preliminary assay of insulin.Young albino rats of 20-50 grams of body weight, with dose of one-hundredth rabbit unit per ten grams of live weight, behave just same as in mice. The older animals over two hundred grams are with the same dose very resistent towards the hypoglycemic convulsions.The insulin convulsions and coma are believed not to occur in pigeon, though the blood sugar level may be easily lowered, with several rabbit units of insulin, to about 50-60% of the normal. The authors describe one rare case of the convulsive reaction in pigeon with two rabbit units dose. The bird convulsed several times during the course of ten hours. The lowest level of the blood sugar was found to be 0, 077 grams per 100c.c.In horse therhypoglycemic convulsions can be easily elicited with one half rabbit units per kilo of body weight. The convulsive blood sugar level was about 0, 050 grams per 100c.c.(4) The efficiency of various sugars in relieving the symptoms caused by insulin in albino rats was studied. The results obtained agree essentially with those of Noble and Macleod in rabbit and of Herring, Irvine and Macleod in mice. Put the quantity of glucose just required (0, 020-0, 025, grams per ten grams of live weight)=1, then mannose=2, maltose=3, fructose>3, galactose>3, and arabinose>4.(5) Insulin exerts no perceptible action on the course of avian beriberi, and vitamin B (oryzanin) has also no influence upon the insulin reactions.","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121298677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ON THE RABBIT-PASSAGE OF RINDERPEST VIRUS","authors":"Tatsu Inoue","doi":"10.1292/JVMS1922.13.314","DOIUrl":"https://doi.org/10.1292/JVMS1922.13.314","url":null,"abstract":"","PeriodicalId":101505,"journal":{"name":"Journal of the Japanese Society of Veterinary Science","volume":"357 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121536613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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