Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1333760
B. Walcott, Mahipal Singh
Abstract The objective of this study was to assess the time limits within which proliferative cells can be recovered in bovine skin stored separately at 4 and 25°C after animal death. In the first experiment, skin explants (n = 110; 2–3 mm2) from 11 animals stored at 4°C were cultured weekly up to 7 weeks in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 50 units/mL of penicillin, 50 μg/mL of streptomycin, and 2.5 μg/mL of fungizone. The presence/absence of fibroblast-like cell outgrowth around explants was scored. Out of 640 explants cultured, 567 (87%) adhered to dish surface, of which 333 (58.73%) exhibited outgrowth including 16.67% explants from 49 days postmortem tissues. Similarly, in the second experiment, when the tissues were stored at 25 ± 2°C prior to culturing on alternate days up to 17 days, 204 (48%) explants exhibited outgrowth that included 19.15% from 15 dpm tissues. The number of explants exhibiting outgrowth was inversely proportional to postmortem time interval in both temperatures studied. Secondary cultures established from outgrowth for selected time points showed stable chromosomes, normal GFP gene expression, and comparable growth morphology to fresh tissue-derived cells. The cells lasted in culture for more than 20 passages. These results suggest that live and usable cells can be recovered from bovine skin tissues up to about 2 weeks postmortem, if skin is stored at 25°C, and about three times more (>6 weeks), if stored at 4°C.
{"title":"Recovery of proliferative cells up to 15- and 49-day postmortem from bovine skin stored at 25°C and 4°C, respectively","authors":"B. Walcott, Mahipal Singh","doi":"10.1080/23312025.2017.1333760","DOIUrl":"https://doi.org/10.1080/23312025.2017.1333760","url":null,"abstract":"Abstract The objective of this study was to assess the time limits within which proliferative cells can be recovered in bovine skin stored separately at 4 and 25°C after animal death. In the first experiment, skin explants (n = 110; 2–3 mm2) from 11 animals stored at 4°C were cultured weekly up to 7 weeks in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 50 units/mL of penicillin, 50 μg/mL of streptomycin, and 2.5 μg/mL of fungizone. The presence/absence of fibroblast-like cell outgrowth around explants was scored. Out of 640 explants cultured, 567 (87%) adhered to dish surface, of which 333 (58.73%) exhibited outgrowth including 16.67% explants from 49 days postmortem tissues. Similarly, in the second experiment, when the tissues were stored at 25 ± 2°C prior to culturing on alternate days up to 17 days, 204 (48%) explants exhibited outgrowth that included 19.15% from 15 dpm tissues. The number of explants exhibiting outgrowth was inversely proportional to postmortem time interval in both temperatures studied. Secondary cultures established from outgrowth for selected time points showed stable chromosomes, normal GFP gene expression, and comparable growth morphology to fresh tissue-derived cells. The cells lasted in culture for more than 20 passages. These results suggest that live and usable cells can be recovered from bovine skin tissues up to about 2 weeks postmortem, if skin is stored at 25°C, and about three times more (>6 weeks), if stored at 4°C.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1333760","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48548785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1370058
Kenneth M. Palanza, Legairre A. Radden, M. Rabah, Tu V. Nguyen, Audra C. Kohm, M. E. Connor, Morgan M C Ricci, Jachius J. Stewart, Sidney Eragene, T. R. King
Abstract Background: The recessive rough fur mutation (ruf)―named for the unkempt, greasy appearance of the hair coat in homozygotes―has previously been mapped on mouse Chromosome 9. However, the assignment of ruf to a particular gene is needed to facilitate a complete molecular analysis of the mutant phenotype. Results: To establish a more refined location for ruf (as a basis for positional cloning) DNA isolated from a large backcross family was typed for microsatellite and single-nucleotide markers on Chromosome 9. This analysis restricted the location of ruf between sites that flank only four genes known to be expressed in skin, one of which―Mpzl3, for myelin protein zero-like 3―generates a similar hair phenotype in mice homozygous for engineered and spontaneous null-alleles. A cross between ruf mutants and mice heterozygous for the Mpzl3rc mutation (which controls a recessive phenotype called rough coat) produced offspring that displayed matted, damp-looking fur, indicating that ruf is a mutant allele of Mpzl3. However, sequence analysis of the Mpzl3 promoter, exons and splice junctions revealed no mutant-specific DNA defect. Conclusion: The results presented indicate that ruf is a mutant allele of Mpzl3. With a genetic assignment in hand, the rough fur variant can now be more fully characterized to advance our understanding of Mpzl3’s role in normal skin development and function, hepatic triglyceride synthesis, weight regulation, energy and glucose homeostasis; and to model-related human disorders.
{"title":"The rough fur (ruf) mutation in mice is an allele of myelin protein zero-like 3 (Mpzl3)","authors":"Kenneth M. Palanza, Legairre A. Radden, M. Rabah, Tu V. Nguyen, Audra C. Kohm, M. E. Connor, Morgan M C Ricci, Jachius J. Stewart, Sidney Eragene, T. R. King","doi":"10.1080/23312025.2017.1370058","DOIUrl":"https://doi.org/10.1080/23312025.2017.1370058","url":null,"abstract":"Abstract Background: The recessive rough fur mutation (ruf)―named for the unkempt, greasy appearance of the hair coat in homozygotes―has previously been mapped on mouse Chromosome 9. However, the assignment of ruf to a particular gene is needed to facilitate a complete molecular analysis of the mutant phenotype. Results: To establish a more refined location for ruf (as a basis for positional cloning) DNA isolated from a large backcross family was typed for microsatellite and single-nucleotide markers on Chromosome 9. This analysis restricted the location of ruf between sites that flank only four genes known to be expressed in skin, one of which―Mpzl3, for myelin protein zero-like 3―generates a similar hair phenotype in mice homozygous for engineered and spontaneous null-alleles. A cross between ruf mutants and mice heterozygous for the Mpzl3rc mutation (which controls a recessive phenotype called rough coat) produced offspring that displayed matted, damp-looking fur, indicating that ruf is a mutant allele of Mpzl3. However, sequence analysis of the Mpzl3 promoter, exons and splice junctions revealed no mutant-specific DNA defect. Conclusion: The results presented indicate that ruf is a mutant allele of Mpzl3. With a genetic assignment in hand, the rough fur variant can now be more fully characterized to advance our understanding of Mpzl3’s role in normal skin development and function, hepatic triglyceride synthesis, weight regulation, energy and glucose homeostasis; and to model-related human disorders.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1370058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43665930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1393864
N. Félix, I. Goy-Thollot, R. S. Walton, Pedro M. Borralho, Hugo Pissara, A. S. Matos, C. Rodrigues, M. Niza
Abstract Purpose: Evaluate if etomidate modulates adrenal apoptosis and if this influences the development of critical illness-related corticosteroid insufficiency (CIRCI) in hemorrhagic shock (HS). Material and methods: Four groups of 16 male Wistar rats: G0 (control group anesthetized with isoflurane and mechanical ventilation), G1 (like G0, but with buprenorphine), G2 (like G1 with HS), and G3 (like G2 with etomidate 1 mg/kg, IV, before HS). HS induced by collecting 30% of blood volume. Resuscitation performed 90 min later with the collected blood and normal saline. Hemodynamic parameters, blood gas analysis, adrenocorticotropic hormone (ACTH), corticosterone (CS), and TNF-α, IL6, IL10 were determined at 0, 90, 150, and 240 min post-HS induction (at the corresponding time points in G0 and G1). Apoptosis and necrosis were determined by TUNEL and caspase-3 immunofluorescence and a necrosis score, respectively. Results: HS groups had significantly higher levels of apoptosis and necrosis than G1 and G0. Compared with G2, etomidate-treated animals had significantly lower levels of CS (compatible with CIRCI), PO2, PO2/FiO2, BE, HCO3, apoptosis, and necrosis and significantly higher cytokine levels. Conclusions: Etomidate was associated with CIRCI. HS was associated with adrenal gland apoptosis and necrosis. The latter were decreased by etomidate, possibly by both direct and indirect mechanisms.
{"title":"Etomidate decreases adrenal gland apoptosis and necrosis associated with hemorrhagic shock in a rat model (Rattus norvegicus)","authors":"N. Félix, I. Goy-Thollot, R. S. Walton, Pedro M. Borralho, Hugo Pissara, A. S. Matos, C. Rodrigues, M. Niza","doi":"10.1080/23312025.2017.1393864","DOIUrl":"https://doi.org/10.1080/23312025.2017.1393864","url":null,"abstract":"Abstract Purpose: Evaluate if etomidate modulates adrenal apoptosis and if this influences the development of critical illness-related corticosteroid insufficiency (CIRCI) in hemorrhagic shock (HS). Material and methods: Four groups of 16 male Wistar rats: G0 (control group anesthetized with isoflurane and mechanical ventilation), G1 (like G0, but with buprenorphine), G2 (like G1 with HS), and G3 (like G2 with etomidate 1 mg/kg, IV, before HS). HS induced by collecting 30% of blood volume. Resuscitation performed 90 min later with the collected blood and normal saline. Hemodynamic parameters, blood gas analysis, adrenocorticotropic hormone (ACTH), corticosterone (CS), and TNF-α, IL6, IL10 were determined at 0, 90, 150, and 240 min post-HS induction (at the corresponding time points in G0 and G1). Apoptosis and necrosis were determined by TUNEL and caspase-3 immunofluorescence and a necrosis score, respectively. Results: HS groups had significantly higher levels of apoptosis and necrosis than G1 and G0. Compared with G2, etomidate-treated animals had significantly lower levels of CS (compatible with CIRCI), PO2, PO2/FiO2, BE, HCO3, apoptosis, and necrosis and significantly higher cytokine levels. Conclusions: Etomidate was associated with CIRCI. HS was associated with adrenal gland apoptosis and necrosis. The latter were decreased by etomidate, possibly by both direct and indirect mechanisms.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1393864","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41538038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1292882
Z. Sawan
Abstract This study investigates the statistical relationship between various climatic factors and overall flower and boll production. Also, the relationship between climatic factors and production of flowers and bolls obtained during the development periods of the flowering and boll stage. Further, predicting effects of climatic factors during different convenient intervals (in days) on cotton flower and boll production compared with daily observations. Furthermore, collects information about the nature of the relationship between various climatic factors and cotton boll development and the 15-day period both prior to and after initiation of individual bolls. And, it provides information on the effect of various climatic factors and soil moisture status during the development stage on flower and boll production in cotton. Evaporation, sunshine duration, relative humidity, surface soil temperature at 18:00 h, and maximum air temperature, are the important climatic factors that significantly affect flower and boll production. The five-day interval was found to be more adequately and sensibly related to yield parameters. Evaporation; minimum humidity, and sunshine duration were the most effective climatic factors during preceding and succeeding periods on boll production and retention. There was a negative correlation between flower and boll production and either evaporation or sunshine duration, while that correlation with minimum relative humidity was positive.
{"title":"Cotton production and climatic factors: Studying the nature of its relationship by different statistical methods","authors":"Z. Sawan","doi":"10.1080/23312025.2017.1292882","DOIUrl":"https://doi.org/10.1080/23312025.2017.1292882","url":null,"abstract":"Abstract This study investigates the statistical relationship between various climatic factors and overall flower and boll production. Also, the relationship between climatic factors and production of flowers and bolls obtained during the development periods of the flowering and boll stage. Further, predicting effects of climatic factors during different convenient intervals (in days) on cotton flower and boll production compared with daily observations. Furthermore, collects information about the nature of the relationship between various climatic factors and cotton boll development and the 15-day period both prior to and after initiation of individual bolls. And, it provides information on the effect of various climatic factors and soil moisture status during the development stage on flower and boll production in cotton. Evaporation, sunshine duration, relative humidity, surface soil temperature at 18:00 h, and maximum air temperature, are the important climatic factors that significantly affect flower and boll production. The five-day interval was found to be more adequately and sensibly related to yield parameters. Evaporation; minimum humidity, and sunshine duration were the most effective climatic factors during preceding and succeeding periods on boll production and retention. There was a negative correlation between flower and boll production and either evaporation or sunshine duration, while that correlation with minimum relative humidity was positive.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1292882","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60091240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1286764
O. Bamidele, J. Ajele, F. M. Olajuyigbe
Abstract This study was conducted to investigate the metabolic defensive mechanism in the larvae of African palm weevil (Rynchophorus phoenicis) administered with dichlorvos (2,2-dichlorovinyl dimethylphosphate) solution. Bioassay experiment with dichlorvos was conducted on the larva and glutathione-utilizing enzyme activities were determined in the major organs: fat body, gut, and head of R. phoenicis larva 48 h after treatment with 0–0.060 μg g−1 body weight dichlorvos solution. Glutathione transferase was purified from the gut of larvae by ion-exchange chromatography on diethylaminoethyl-Sephadex A50 and affinity chromatography on glutathione-Sepharose 4B columns. The purified enzyme was homogenous as revealed by sodium dodecylsulfate polyacrylamide gel electrophoresis. Initial velocity studies were carried out on the purified enzyme using standard procedures. Bioassay experiment indicated alterations of glutathione peroxidase, glutathione reductase, and glutathione transferase activities in the major organs of larva caused by dichlorvos. Glutathione transferase activity in the gut of larva was three times higher than that of glutathione peroxidase and glutathione reductase activities, an indication of possible detoxification role of glutathione transferase in the organ. A 49.7 kDa homodimeric glutathione transferase was identified from the gut of larva and was tagged rplGSTc. Mechanism of action of rplGSTc with 1-chloro-2,4-dinitrobenzene, and glutathione as substrates conformed to the random sequential mechanism. These results confirmed the presence of GST associated with the degradation of dichlorvos in the gut of R. phoenicis larva.
{"title":"An evaluation of glutathione transferase associated with Dichlorvos degradation in African palm weevil (Rynchophorus phoenicis) larva","authors":"O. Bamidele, J. Ajele, F. M. Olajuyigbe","doi":"10.1080/23312025.2017.1286764","DOIUrl":"https://doi.org/10.1080/23312025.2017.1286764","url":null,"abstract":"Abstract This study was conducted to investigate the metabolic defensive mechanism in the larvae of African palm weevil (Rynchophorus phoenicis) administered with dichlorvos (2,2-dichlorovinyl dimethylphosphate) solution. Bioassay experiment with dichlorvos was conducted on the larva and glutathione-utilizing enzyme activities were determined in the major organs: fat body, gut, and head of R. phoenicis larva 48 h after treatment with 0–0.060 μg g−1 body weight dichlorvos solution. Glutathione transferase was purified from the gut of larvae by ion-exchange chromatography on diethylaminoethyl-Sephadex A50 and affinity chromatography on glutathione-Sepharose 4B columns. The purified enzyme was homogenous as revealed by sodium dodecylsulfate polyacrylamide gel electrophoresis. Initial velocity studies were carried out on the purified enzyme using standard procedures. Bioassay experiment indicated alterations of glutathione peroxidase, glutathione reductase, and glutathione transferase activities in the major organs of larva caused by dichlorvos. Glutathione transferase activity in the gut of larva was three times higher than that of glutathione peroxidase and glutathione reductase activities, an indication of possible detoxification role of glutathione transferase in the organ. A 49.7 kDa homodimeric glutathione transferase was identified from the gut of larva and was tagged rplGSTc. Mechanism of action of rplGSTc with 1-chloro-2,4-dinitrobenzene, and glutathione as substrates conformed to the random sequential mechanism. These results confirmed the presence of GST associated with the degradation of dichlorvos in the gut of R. phoenicis larva.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1286764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47133427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1336886
Xuesheng Han
Abstract Although essential oils from Douglas fir are popular topical skincare products, research regarding their biological effects on human skin cells is scarce. Here, we studied the biological activity of a commercially available Douglas fir (Pseudotsuga menziesii) essential oil (DEO) in a human dermal fibroblast model of chronic inflammation and fibrosis induced by stimulation with cytokines. Chemical analysis of DEO indicated that its major chemical components (i.e. >5%) were beta-pinene (23%), sabinene (17%), terpinolene (14%), delta-3-carene (11%), and alpha-pinene (9%). We analyzed the effect of DEO on the levels of 17 important protein biomarkers associated with inflammation, immune system modulation, and tissue remodeling. DEO exhibited significant anti-proliferative activity in human fibroblasts. DEO also significantly inhibited the production of vascular cell adhesion molecule 1, collagen III, and plasminogen activator inhibitor 1. We also observed that DEO robustly modulated global gene expression levels in diverse ways. In particular, DEO affected the expression of genes involved in immune modulation and cancer signaling. This study provides the first evidence of biological activity of DEO in human dermal fibroblasts. Our results suggest that DEO may modulate immune responses and tumor signaling processes. Further research about the biological and pharmacological mechanisms of DEO action is recommended.
{"title":"In vitro biological activities of Douglas fir essential oil in a human skin disease model","authors":"Xuesheng Han","doi":"10.1080/23312025.2017.1336886","DOIUrl":"https://doi.org/10.1080/23312025.2017.1336886","url":null,"abstract":"Abstract Although essential oils from Douglas fir are popular topical skincare products, research regarding their biological effects on human skin cells is scarce. Here, we studied the biological activity of a commercially available Douglas fir (Pseudotsuga menziesii) essential oil (DEO) in a human dermal fibroblast model of chronic inflammation and fibrosis induced by stimulation with cytokines. Chemical analysis of DEO indicated that its major chemical components (i.e. >5%) were beta-pinene (23%), sabinene (17%), terpinolene (14%), delta-3-carene (11%), and alpha-pinene (9%). We analyzed the effect of DEO on the levels of 17 important protein biomarkers associated with inflammation, immune system modulation, and tissue remodeling. DEO exhibited significant anti-proliferative activity in human fibroblasts. DEO also significantly inhibited the production of vascular cell adhesion molecule 1, collagen III, and plasminogen activator inhibitor 1. We also observed that DEO robustly modulated global gene expression levels in diverse ways. In particular, DEO affected the expression of genes involved in immune modulation and cancer signaling. This study provides the first evidence of biological activity of DEO in human dermal fibroblasts. Our results suggest that DEO may modulate immune responses and tumor signaling processes. Further research about the biological and pharmacological mechanisms of DEO action is recommended.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1336886","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49087619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1394510
Clarissa Silva Lima, Uriel David de Almeida e Silva, Larissa Daniele Machado Góes, Beatriz Martins de Sá Hyacienth, Helison de Oliveria Carvalho, Caio Pinho Fernandes, Andrés Navarrete Castro, J. C. Tavares Carvalho
Abstract The toxic effects of copaíba oil-resin (ORC) and the copaiba oil-resin vaginal cream (CVC) were evaluated in subacute treatment phase, in wistar rats, which were treated orally (p.o) and intravaginal (ivag). Treated groups received the dose of, 0.04 mg/kg, 28 mg/kg, and 32 mg/kg, respectively, with ORC (p.o), Copaifera duckei oil-resin (ORCV-ivag) and vaginal cream with oil-resin C. duckei (CVC – ivag). The treatment for 22 days with ORC and CVC did not cause clinical signs of toxicity, no deaths have been reported, and did not change the development weight of the animals. The females treated with ORC (p.o), exhibited greater intake of water, but the feed intake was not different in males and females. The use of CVC did not change the water intake of females, but altered feed intake. The ORCV was capable of causing hypoglycemic effect and elevated serum creatinine levels. The hematology parameters of the animals were not changed by ORC (p.o, 32 mg/kg). The use of CVC changed hematocrit, lymphocytes, and the concentration of hemoglobin. The use of CVC and ORCV (ivag), did not altered the biochemicals parameters. Females treated with ORC (p.o, 32 mg/kg) showed some kind of susceptibility to specific use (elevation of total cholesterol, HDL and alkaline phosphatase). The elevation of serum ALT and AST enzymes was not attributed to the use of ORC orally and intravaginal and the use of CVC, as well as the product of its formulation (BVC). The subacute treatment with C. duckei oil-resin and cream (CVC) did not cause clinical signs of toxicity, no deaths have been reported and did not change significantly the parameters evaluated in this study.
{"title":"Non-clinical toxicity study of the oil-resin and vaginal cream of Copaiba (Copaifera duckei, Dwyer)","authors":"Clarissa Silva Lima, Uriel David de Almeida e Silva, Larissa Daniele Machado Góes, Beatriz Martins de Sá Hyacienth, Helison de Oliveria Carvalho, Caio Pinho Fernandes, Andrés Navarrete Castro, J. C. Tavares Carvalho","doi":"10.1080/23312025.2017.1394510","DOIUrl":"https://doi.org/10.1080/23312025.2017.1394510","url":null,"abstract":"Abstract The toxic effects of copaíba oil-resin (ORC) and the copaiba oil-resin vaginal cream (CVC) were evaluated in subacute treatment phase, in wistar rats, which were treated orally (p.o) and intravaginal (ivag). Treated groups received the dose of, 0.04 mg/kg, 28 mg/kg, and 32 mg/kg, respectively, with ORC (p.o), Copaifera duckei oil-resin (ORCV-ivag) and vaginal cream with oil-resin C. duckei (CVC – ivag). The treatment for 22 days with ORC and CVC did not cause clinical signs of toxicity, no deaths have been reported, and did not change the development weight of the animals. The females treated with ORC (p.o), exhibited greater intake of water, but the feed intake was not different in males and females. The use of CVC did not change the water intake of females, but altered feed intake. The ORCV was capable of causing hypoglycemic effect and elevated serum creatinine levels. The hematology parameters of the animals were not changed by ORC (p.o, 32 mg/kg). The use of CVC changed hematocrit, lymphocytes, and the concentration of hemoglobin. The use of CVC and ORCV (ivag), did not altered the biochemicals parameters. Females treated with ORC (p.o, 32 mg/kg) showed some kind of susceptibility to specific use (elevation of total cholesterol, HDL and alkaline phosphatase). The elevation of serum ALT and AST enzymes was not attributed to the use of ORC orally and intravaginal and the use of CVC, as well as the product of its formulation (BVC). The subacute treatment with C. duckei oil-resin and cream (CVC) did not cause clinical signs of toxicity, no deaths have been reported and did not change significantly the parameters evaluated in this study.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1394510","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60091319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.1080/23312025.2017.1295823
Lidianne Salvatierra, M. Q. Almeida
Abstract The first occurrence of a parasitic mite, Leptus Latreille (Trombidiformes, Erythraeidae) parasitizing an adult male of a trapdoor spider Actinopus Perty, 1833 (Araneae: Mygalomorphae: Actinopodidae) and the first occurrence of Leptus sp. larvae in the municipality of Manaus, Amazonas state, Brazil are reported.
{"title":"First record of a Leptus Latreille mite (Trombidiformes, Erythraeidae) associated with a Neotropical trapdoor spider (Araneae: Mygalomorphae: Actinopodidae)","authors":"Lidianne Salvatierra, M. Q. Almeida","doi":"10.1080/23312025.2017.1295823","DOIUrl":"https://doi.org/10.1080/23312025.2017.1295823","url":null,"abstract":"Abstract The first occurrence of a parasitic mite, Leptus Latreille (Trombidiformes, Erythraeidae) parasitizing an adult male of a trapdoor spider Actinopus Perty, 1833 (Araneae: Mygalomorphae: Actinopodidae) and the first occurrence of Leptus sp. larvae in the municipality of Manaus, Amazonas state, Brazil are reported.","PeriodicalId":10412,"journal":{"name":"Cogent Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23312025.2017.1295823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42089849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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