Multi-agent AI systems, which simulate diverse instructional roles such as teachers and peers, offer new possibilities for personalized and interactive learning. Yet, the specific patterns of student-AI interaction and their pedagogical implications remain unclear. This study explores how university students engaged with multiple AI agents during a six-module course, and how these interactions influenced cognitive outcomes (learning gains) and non-cognitive factors (motivation, technology acceptance). Through the analysis of dialogue data, two core engagement patterns were identified: co-construction of knowledge and co-regulation. Students with lower prior knowledge relied more on co-construction of knowledge sequences and showed higher learning gains and post-course motivation. In contrast, students with higher prior knowledge engaged more in co-regulation behaviors but demonstrated limited learning improvement. Technology acceptance increased across all groups. These findings suggest that multi-agent systems can effectively support differentiated engagement and help reduce performance gaps by adapting to students' varying needs. This study makes three innovative contributions to existing research: it is based on a long-term formal curriculum, moving beyond fragmented, short-term interactions; it investigates a multi-agent collaborative mechanism that simulates diverse pedagogical roles (e.g., AI teacher, AI teaching assistance, AI classmates), distinguishing this work from single-agent systems; and it explores differentiated interaction modes based on prior knowledge, providing critical teaching implications for personalized learning.
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