Current Developments in Nutrition最新文献

{"title":"Corrigendum to: Single Dose of Intravenous Ferric Carboxymaltose Prevents Anemia for 6 Months among Moderately or Severely Anemic Postpartum Women: A Case Study from India. Curr Dev Nutr. 2021; 5(7): nzab078. doi: 10.1093/cdn/nzab078","authors":"Ravneet Kaur , Shashi Kant , Partha Haldar , Farhad Ahamed , Archana Singh , Vignesh Dwarakanathan , Sumit Malhotra , Kapil Yadav","doi":"10.1016/j.cdnut.2025.107626","DOIUrl":"10.1016/j.cdnut.2025.107626","url":null,"abstract":"","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107626"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Safranal Against Spike Protein-Induced Mitochondrial Dysfunction and Inflammation in Peripheral and Central Immune Cells","authors":"Antonella Girgenti ,&nbsp;Martina Letizia Contente ,&nbsp;Miriam Buttacavoli ,&nbsp;Laura Palumbo ,&nbsp;Flores Naselli ,&nbsp;Sabrina Dallavalle ,&nbsp;Gigliola Borgonovo ,&nbsp;Pasquale Picone ,&nbsp;Andrea Pinto ,&nbsp;Domenico Nuzzo","doi":"10.1016/j.cdnut.2025.107629","DOIUrl":"10.1016/j.cdnut.2025.107629","url":null,"abstract":"<div><h3>Background</h3><div>Saffron (<em>Crocus sativus L</em>.) contains bioactive molecules with antioxidant, anti-inflammatory, and neuroprotective properties. Growing evidence indicates that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) promotes neuroinflammation and mitochondrial dysfunction contributing to neuro-coronavirus disease.</div></div><div><h3>Objectives</h3><div>The aim of this study is to evaluate the antioxidant, anti-inflammatory, and neuroprotective effects of 3 saffron derivatives, picrocrocin, 4-hydroxysafranal, and safranal, in peripheral immune cells and microglia, and to test the hypothesis that these compounds, especially safranal, counteract Spike protein 1(S1)-induced inflammation and mitochondrial dysfunction.</div></div><div><h3>Methods</h3><div>An immortalized murine microglial cell line (BV2) and human peripheral blood mononuclear cells (PBMCs) from healthy donors were treated with saffron derivatives at nontoxic concentrations (0.05–0.5 mM). Cytotoxicity (3-(4,5-dimethylthiazol-2-yl)-5-(3‑carboxymethoxyphenyl)-2-(4‑sulfophenyl)-2H‑tetrazolium (MTS) assay), antioxidant capacity [2,2-diphenyl-1-picrylhydrazyl (DPPH)], intracellular reactive oxygen species (ROS; 2,7-dichlorodihydrofluorescein diacetate), cytokine expression (enzyme-linked immunosorbent assay and quantitative polymerase chain reaction), and mitochondrial membrane potential (5,5′,6,6′‑tetrachloro‑1,1′,3,3′‑tetraethylbenzimidazolylcarbocyanine iodide (JC-1) assay) were assessed. Lipopolysaccharide (LPS) served as an inflammatory control, whereas S1 was used to model SARS-CoV-2-mediated neuroinflammation and mitochondrial damage.</div></div><div><h3>Results</h3><div>All saffron derivatives showed antioxidant activity, with safranal demonstrating the strongest DPPH radical scavenging effect and the most pronounced reduction of intracellular ROS. In LPS-stimulated BV2 cells, safranal significantly decreased inducible nitric oxide synthase expression. In PBMCs, saffron compounds attenuated LPS-induced interleukin-1 beta (IL-1β) release, with safranal showing the greatest decrease. S1 increased IL-1β and tumor necrosis factor-alpha expression in BV2 microglia. Co-treatment with safranal reduced these cytokines by ∼38% and 44%, respectively. S1 induced a loss of mitochondrial membrane potential, which was effectively restored by safranal, as confirmed by JC-1 fluorescence analysis.</div></div><div><h3>Conclusions</h3><div>These findings identify safranal as a promising neuroprotective candidate for preventing or mitigating SARS-CoV-2-associated neurological damage and other disorders involving microglial activation and mitochondrial impairment.</div></div>","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107629"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Economic Implications of Relapse Among Children Recovered from Severe Acute Malnutrition: Results from a Multicountry Prospective Study in Mali, Somalia, and South Sudan","authors":"Chloe Puett ,&nbsp;Sarah King ,&nbsp;Sherifath Mama Chabi ,&nbsp;Feysal Abdisalan Mohamud ,&nbsp;Nancy Lamwaka ,&nbsp;Heather Stobaugh","doi":"10.1016/j.cdnut.2025.107616","DOIUrl":"10.1016/j.cdnut.2025.107616","url":null,"abstract":"<div><h3>Background</h3><div>The community-based management of acute malnutrition (CMAM) is effective at recovering children from severe acute malnutrition (SAM) and saving lives. However, postdischarge, children may relapse to acute malnutrition (AM), thereby requiring retreatment.</div></div><div><h3>Objectives</h3><div>This study aims to assess the economic implications of treating children who relapse to AM within 6 mo of recovering from initial treatment for uncomplicated SAM in CMAM programs.</div></div><div><h3>Methods</h3><div>This study was part of a multicountry prospective cohort study in which children aged 6–47 mo with uncomplicated SAM were followed for 6 mo after recovery in Mali, Somalia, and South Sudan (SSD). Institutional cost data were collected monthly through accounting records or key informant interviews, depending on data availability. Mean costs per treatment episode for each CMAM program component were calculated (initial SAM recovery, relapse to SAM treatment, relapse to moderate AM treatment; 2022 USD).</div></div><div><h3>Results</h3><div>The cost per child of recovery in initial SAM treatment was $191, $92, and $178 in Mali, Somalia, and SSD, respectively. The cost per child who failed to sustain recovery and required treatment for relapse to SAM was 1.7–2.2 times higher than initial recovery, amounting to an additional $215, $64, and $215/child in Mali, Somalia, and SSD, respectively. Not having to retreat children would save per admitted child on mean $2 in Somalia, $26 in SSD, and $45 in Mali.</div></div><div><h3>Conclusions</h3><div>This is the first analysis to estimate the cost of retreating children who relapse to AM within 6 mo of recovering from SAM. Our findings show that the cost of treating relapse to SAM is, on mean, twice as high as the initial SAM recovery. Preventing relapse could free up significant resources. Amid reduced global assistance, these results highlight the economic rationale for improving the efficiency of global nutrition programs by reducing relapse rates and strengthening prevention strategies.</div></div>","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107616"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex Differences in Measures of Energy Expenditure and Body Composition in Young, Middle-Aged, and Older Adults","authors":"Hannah E Cabre ,&nbsp;Kara L Marlatt ,&nbsp;Rodrigo Fernández-Verdejo ,&nbsp;Robbie Beyl ,&nbsp;Leanne M Redman ,&nbsp;Philip N Ainslie ,&nbsp;Heliodoro Alemán-Mateo ,&nbsp;Lene F Andersen ,&nbsp;Liam J Anderson ,&nbsp;Lenore Arab ,&nbsp;Kweku Bedu-Addo ,&nbsp;Alberto G Bonomi ,&nbsp;Carlijn VC Bouten ,&nbsp;Pascal Bovet ,&nbsp;Soren Brage ,&nbsp;Maciej S Buchowski ,&nbsp;Nancy F Butte ,&nbsp;Stefan G Camps ,&nbsp;Regina Casper ,&nbsp;Graeme L Close ,&nbsp;Eric Ravussin","doi":"10.1016/j.cdnut.2025.107614","DOIUrl":"10.1016/j.cdnut.2025.107614","url":null,"abstract":"<div><h3>Background</h3><div>Total daily energy expenditure (TDEE) is vital for energy balance and cardiometabolic health, yet its trajectory across the lifespan, particularly in females, remains poorly understood.</div></div><div><h3>Objectives</h3><div>We sought to examine the effects of aging and sex on body composition and TDEE.</div></div><div><h3>Methods</h3><div>In a cross-sectional analysis of data from research centers across 9 European Countries and the United States from the International Atomic Energy Agency database, TDEE and body composition measures of 2326 participants (1560W/766M; 50.7 ± 12 .6 y) were stratified across age groups: young (30–39 y; YOUNG), middle-aged (40–54 y; MID), and old (55–70 y; OLD). Doubly labeled water was used to estimate TDEE and fat-free mass (FFM). Fat mass (FM) was calculated as the difference between body mass and FFM, and %fat was ratio between FM and body mass as a percentage. Linear models were used for analysis.</div></div><div><h3>Results</h3><div>Females demonstrated greater FM and lower FFM with each age group, compared with males (<em>P &lt;</em> 0.001). In females, OLD had lower absolute TDEE than YOUNG (−217 kcal/d, <em>P &lt;</em> 0.001) and MID (−208 kcal/d, <em>P &lt;</em> 0.001). Male absolute TDEE was lowered across all age groups (OLD compared with YOUNG: −334 kcal/d; OLD compared with MID: −210 kcal/d; MID compared with YOUNG: −124 kcal/d; <em>P &lt;</em> 0.001). Adjusted TDEE was similar within age groups between females and males.</div></div><div><h3>Conclusions</h3><div>These results suggest that age influences changes in body composition and energy expenditure similarly between males and females. The most significant change in TDEE occurs as individuals transition from middle age to older adulthood. Females generally have a higher percentage of %fat and FM, along with lower FFM, compared with males across all age groups. These findings are important for understanding how aging affects metabolism and body composition, which could inform sex-specific health strategies and interventions.</div></div>","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107614"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sports Nutrition","authors":"Jonathan D Buckley ,&nbsp;Jess Gwin ,&nbsp;Stuart Phillips","doi":"10.1016/j.cdnut.2025.107613","DOIUrl":"10.1016/j.cdnut.2025.107613","url":null,"abstract":"","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107613"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calculation of Compound Intake Levels Using Integrated Food Compound Databases and Food Intake Data","authors":"Marie Y Meima ,&nbsp;Joost Westerhout ,&nbsp;Sabina Bijlsma ,&nbsp;Fiona DM van Schaik ,&nbsp;Bas Oldenburg ,&nbsp;Marjo JE Campmans-Kuijpers ,&nbsp;Marjolein Meijerink ,&nbsp;Geert F Houben","doi":"10.1016/j.cdnut.2025.107612","DOIUrl":"10.1016/j.cdnut.2025.107612","url":null,"abstract":"<div><h3>Background</h3><div>Despite substantial research, the mechanisms through which food influences disease largely remain unclear. Analyzing cohort data at the level of food compounds (i.e. individual molecules present in foods) may reveal new insights, and as a first step, we previously integrated 3 food compound databases.</div></div><div><h3>Objectives</h3><div>This study aimed to combine the integrated food compound databases with food intake data to estimate compound intake levels and evaluate their plausibility.</div></div><div><h3>Methods</h3><div>We used food frequency questionnaire (FFQ) records from 135 adult patients with inflammatory bowel disease from University Medical Center Utrecht. Generic FFQ-based dietary data were translated into compound intake values. We dealt with differing compound values across the databases by applying a systematic prioritization strategy to obtain a single representative value per compound in each food item. Intake amounts of 770 compounds were calculated for all 135 subjects. For vitamins and minerals, plausibility was assessed by checking whether the p50 of our calculated intake fell within the p5 to p95 range of Dutch population intake data. For fatty acids and polyphenols, plausibility was assessed by evaluating whether percentile ranges overlapped with those reported in literature.</div></div><div><h3>Results</h3><div>Our findings indicate that all p50 values of our calculated intake data fell within the p5 to p90 range of the Dutch population intake data for vitamins and minerals. For fatty acids and polyphenols, intake ranges overlapped with those reported in literature for all compounds, but showed more deviation, likely due to regional dietary differences and the absence of standardized population-based intake benchmarks.</div></div><div><h3>Conclusions</h3><div>Our study provides a foundation for food-health research by offering plausible intake estimates for a wide range of dietary compounds derived from Dutch cohort data.</div></div>","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107612"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eliminate the In Vivo Digestibility Requirement for Protein Content Claims in North America to Align Consumer Purchasing Behavior with Dietary Guidelines","authors":"Joseph Manuppello ,&nbsp;Christopher D Gardner ,&nbsp;Anna Herby ,&nbsp;Elaine S Krul ,&nbsp;Christopher PF Marinangeli ,&nbsp;Amanda Gomes Almeida Sá ,&nbsp;Mingyang Song","doi":"10.1016/j.cdnut.2025.107627","DOIUrl":"10.1016/j.cdnut.2025.107627","url":null,"abstract":"<div><div>A roundtable discussion, held on 10 December, 2024, addressed requirements for protein quality assessment in United States and Canadian food labeling regulations, focusing on concerns with the protein digestibility-corrected amino acid score (PDCAAS), which includes an in vivo rat assay to determine true fecal protein digestibility. Because animal proteins tend to score higher, the PDCAAS disadvantages nonanimal foods in substantiating protein content claims despite dietary guidelines recommending increased intake of plant proteins. In addition, the use of animal testing raises ethical concerns for some consumers. Roundtable participants weighed the benefits and costs of requiring the PDCAAS and discussed alternative regulatory approaches to better promote human health, prevent chronic disease, replace animal testing, and support sustainable food production. Options included relying solely on the amount of protein per serving, correcting only for the amino acid score, using fixed coefficients of digestibility or in vitro assays to determine digestibility, and incorporating measures that reflect human health outcomes and environmental impact. Several in vitro methods, such as the pH-drop and pH-stat methods, were identified as promising candidates for regulatory acceptance. The consensus was that for foods that do not address special needs, relying solely on the amount of protein to substantiate content claims is appropriate for populations who already consume protein in excess of reference values from varied sources. This approach, already used in other high-income jurisdictions, allows more plant-based foods to qualify for protein claims while avoiding animal testing. Moving away from the in vivo derived PDCAAS would reduce existing regulatory barriers, better align with current dietary guidelines, and promote increased intake of plant-based foods, thereby improving public health and sustainability.</div></div>","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 2","pages":"Article 107627"},"PeriodicalIF":3.2,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145986697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to: The Role of Diet and Hormones on Taste: Low Carb Compared With Low Fat Study Findings. Curr Dev Nutr. 2025 May 19;9(6):107467. doi: 10.1016/j.cdnut.2025.107467","authors":"Rosario B Jaime-Lara ,&nbsp;Alexis T Franks ,&nbsp;Nafisa Nawal ,&nbsp;Meaghan C Steck ,&nbsp;Ariana M Chao ,&nbsp;Carolyn Allen ,&nbsp;Brianna E Brooks ,&nbsp;Monica Atkinson ,&nbsp;Amber B Courville ,&nbsp;Juen Guo ,&nbsp;Shanna Yang ,&nbsp;Marinza Marzouk ,&nbsp;Valerie L Darcey ,&nbsp;Stephanie Chung ,&nbsp;Ciarán G Forde ,&nbsp;Kevin D Hall ,&nbsp;Paule V Joseph","doi":"10.1016/j.cdnut.2025.107618","DOIUrl":"10.1016/j.cdnut.2025.107618","url":null,"abstract":"","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107618"},"PeriodicalIF":3.2,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Minimally Processed Red Meat within a Plant-Forward Diet on Biomarkers of Physical and Cognitive Aging: A Randomized Controlled Crossover Feeding Trial","authors":"Saba Vaezi ,&nbsp;Bruna O de Vargas ,&nbsp;Lee Weidauer ,&nbsp;Jessica L Freeling ,&nbsp;Moul Dey","doi":"10.1016/j.cdnut.2025.107615","DOIUrl":"10.1016/j.cdnut.2025.107615","url":null,"abstract":"<div><h3>Background</h3><div>Popular dietary patterns for cardiovascular and cognitive health often emphasize limiting red meat intake. However, evidence specifically examining the effects of minimally processed lean red meat, independent of processed varieties, remains limited.</div></div><div><h3>Objectives</h3><div>This study aims to evaluate the impact of incorporating minimally processed lean red meat into a plant-forward dietary pattern aligned with the 2020–2025 Dietary Guidelines for Americans on markers of age-related cognitive, metabolic, and physical health.</div></div><div><h3>Methods</h3><div>This 18-wk randomized controlled crossover feeding trial tested a red meat diet with 162 g/d minimally processed pork (MPP) against a macronutrient- and energy-matched no-meat control diet with minimally processed lentils (MPL) in ≥65 y older adults. Serum biomarkers relevant to metabolic-related cognitive and physical health were explored. Primary and secondary endpoints comprised 5 cardiovascular markers, 12 nutrition and neurotransmitter measures, and 2 metrics each of body composition and muscular fitness. Data were analyzed with robust mixed-effects models adjusted for covariates.</div></div><div><h3>Results</h3><div>Thirty-six Midwestern older adults (26/10 females/males; mean age 71.7 y; mean body mass index: 28 kg/m²) completed the study. Cognitive-related metabolic biomarkers improved across both arms. Adoption of a plant-forward diet led to a reduction in fasting insulin after both MPP and MPL phases (<em>P</em> &lt; 0.001), and Single Point Insulin Sensitivity Estimator Index increased after MPP (<em>P</em> = 0.032), with no significant between-diet differences. High-density lipoprotein concentrations were higher post-MPP than post-MPL (<em>P</em> = 0.034). Body weight decreased in both phases (<em>P</em> &lt; 0.05), with a trend toward smaller lean mass loss post-MPP. Grip strength and chair-rise performance were maintained throughout the intervention. Neuroactive metabolites and bioactive amino acid concentrations shifted favorably after both interventions.</div></div><div><h3>Conclusions</h3><div>Findings challenge the perception that red meat is broadly unsuitable for older adults. Including familiar foods like red meat (pork), particularly in minimally processed form and within a healthy overall dietary pattern, may provide age-associated health benefits and improve adherence to plant-forward diets in populations where red meat remains popular.</div><div>This trial was registered at <span><span>clinicaltrials.gov</span><svg><path></path></svg></span> as NCT05581953 (12 October, 2022) and NCT06261775 (7 February, 2024).</div></div>","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"10 1","pages":"Article 107615"},"PeriodicalIF":3.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Replacing Added Sugars with Sucralose on Gut Microbiome Composition Among Asian Indian Adults in Two 12-week Randomized Controlled Trials","authors":"Danielle E Haslam ,&nbsp;Kuzhandaivelu Abirami ,&nbsp;Jacqueline R Starr ,&nbsp;Ranjit Unnikrishnan ,&nbsp;Jessica Lasky-Su ,&nbsp;Rajagopal Gayathri ,&nbsp;Kuppan Gokulakrishnan ,&nbsp;Valangaiman Sriram Manasa ,&nbsp;Eric B Rimm ,&nbsp;Ranjit Mohan Anjana ,&nbsp;Kamala Krishnaswamy ,&nbsp;Frank B Hu ,&nbsp;Vasudevan Sudha ,&nbsp;Viswanathan Mohan ,&nbsp;Shilpa N Bhupathiraju","doi":"10.1016/j.cdnut.2025.107600","DOIUrl":"10.1016/j.cdnut.2025.107600","url":null,"abstract":"<div><h3>Background</h3><div>Replacing added sugars with nonnutritive sweeteners, such as sucralose, may help reduce weight gain in adults over time. Because sucralose is primarily excreted in the stool, its consumption could lead to changes in the gut microbiome.</div></div><div><h3>Objectives</h3><div>We aimed to explore whether replacing sucrose used in beverages with small quantities of sucralose led to gut microbiome changes among Asian Indian adults with type 2 diabetes (T2D) or overweight/obesity (BMI ≥23 kg/m²) without T2D.</div></div><div><h3>Methods</h3><div>In 2 analogous substudies nested within two 12-wk, open-label parallel-arm randomized controlled trials, adults with T2D (n = 49) or overweight/obesity and no T2D (n = 48) were instructed to replace sucrose in their daily coffee and tea with sucralose or to continue their use of sucrose. We examined changes in gut microbiome community structure and taxonomic composition profiled using 16S rRNA sequencing in stool samples collected before and after the 12-wk interventions. The false discovery rate was controlled using the Benjamini-Hochberg method (q &lt; 0.20).</div></div><div><h3>Results</h3><div>Compared with the control group, the sucralose intervention decreased α diversity (Shannon index: <em>P</em> = 0.02; Simpson index: <em>P</em> = 0.03) and increased β diversity (<em>P</em> = 0.001) in gut microbiome communities of adults with T2D, but not among adults with overweight/obesity (all between-group <em>P</em> &gt; 0.05). Among 185 genera tested in the T2D trial, compared with the control, relative abundances of 14 primarily sugar-fermenting or short-chain fatty-acid-producing <em>Firmicutes</em> bacteria in the <em>Lachnospiracae</em> family were reduced, whereas <em>Enterococcus</em> and <em>Pediococcus</em> increased during the intervention (q &lt; 0.20). In contrast, adults with overweight/obesity and no T2D showed no similar changes.</div></div><div><h3>Conclusions</h3><div>Replacing daily sucrose added to coffee and tea with sucralose resulted in changes in gut microbiome community structure and taxonomic composition among Asian Indian adults with T2D, but not those with overweight/obesity and no T2D. Further studies are needed to understand potential health implications and the underlying drivers of these gut microbiome changes.</div><div>Clinical Trial Register No. (India Trial Register): CTRI/2021/04/032686, CTRI/2021/04/032809.</div></div>","PeriodicalId":10756,"journal":{"name":"Current Developments in Nutrition","volume":"9 12","pages":"Article 107600"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}