首页 > 最新文献

Current protein & peptide science最新文献

英文 中文
Global Emergence of SARS-CoV2 Infection and Scientific Interventions to Contain its Spread. 全球出现的 SARS-CoV2 感染和遏制其传播的科学干预措施。
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/0113892037274719231212044235
Himanshu Ajmera, Sudarshan Singh Lakhawat, Naveen Malik, Akhilesh Kumar, Jasvinder Singh Bhatti, Vikram Kumar, Himanshu Gogoi, Sunil Kumar Jaswal, Sanjeev Chandel, Pushpender Kumar Sharma

The global pandemic caused by COVID-19 posed a significant challenge to public health, necessitating rapid scientific interventions to tackle the spread of infection. The review discusses the key areas of research on COVID-19 including viral genomics, epidemiology, pathogenesis, diagnostics, and therapeutics. The genome sequencing of the virus facilitated the tracking of its evolution, transmission dynamics, and identification of variants. Epidemiological studies have provided insights into disease spread, risk factors, and the impact of public health infrastructure and social distancing measures. Investigations of the viral pathogenesis have elucidated the mechanisms underlying immune responses and severe manifestations including the long-term effects of COVID-19. Overall, the article provides an updated overview of the diagnostic methods developed for SARS-CoV-2 and discusses their strengths, limitations, and appropriate utilization in different clinical and public health settings. Furthermore, therapeutic approaches including antiviral drugs, immunomodulatory therapies, and repurposed medications have been investigated to alleviate disease severity and improve patient outcomes. Through a comprehensive analysis of these scientific efforts, the review provides an overview of the advancements made in understanding and tackling SARS-CoV-2, while underscoring the need for continued research to address the evolving challenges posed by this global health crisis.

由 COVID-19 引起的全球大流行对公共卫生构成了重大挑战,需要迅速采取科学干预措施来解决感染传播问题。本综述讨论了 COVID-19 的关键研究领域,包括病毒基因组学、流行病学、发病机制、诊断学和治疗学。该病毒的基因组测序有助于追踪其进化、传播动态和变异体的鉴定。流行病学研究有助于深入了解疾病的传播、风险因素以及公共卫生基础设施和社会隔离措施的影响。对病毒发病机制的调查阐明了免疫反应和严重表现(包括 COVID-19 的长期影响)的基本机制。总之,文章概述了针对 SARS-CoV-2 开发的最新诊断方法,并讨论了这些方法的优势、局限性以及在不同临床和公共卫生环境中的适当使用。此外,还研究了包括抗病毒药物、免疫调节疗法和再利用药物在内的治疗方法,以减轻疾病的严重程度并改善患者的预后。本综述通过对这些科研工作的全面分析,概述了在了解和应对 SARS-CoV-2 方面取得的进展,同时强调了继续开展研究以应对这一全球健康危机所带来的不断变化的挑战的必要性。
{"title":"Global Emergence of SARS-CoV2 Infection and Scientific Interventions to Contain its Spread.","authors":"Himanshu Ajmera, Sudarshan Singh Lakhawat, Naveen Malik, Akhilesh Kumar, Jasvinder Singh Bhatti, Vikram Kumar, Himanshu Gogoi, Sunil Kumar Jaswal, Sanjeev Chandel, Pushpender Kumar Sharma","doi":"10.2174/0113892037274719231212044235","DOIUrl":"10.2174/0113892037274719231212044235","url":null,"abstract":"<p><p>The global pandemic caused by COVID-19 posed a significant challenge to public health, necessitating rapid scientific interventions to tackle the spread of infection. The review discusses the key areas of research on COVID-19 including viral genomics, epidemiology, pathogenesis, diagnostics, and therapeutics. The genome sequencing of the virus facilitated the tracking of its evolution, transmission dynamics, and identification of variants. Epidemiological studies have provided insights into disease spread, risk factors, and the impact of public health infrastructure and social distancing measures. Investigations of the viral pathogenesis have elucidated the mechanisms underlying immune responses and severe manifestations including the long-term effects of COVID-19. Overall, the article provides an updated overview of the diagnostic methods developed for SARS-CoV-2 and discusses their strengths, limitations, and appropriate utilization in different clinical and public health settings. Furthermore, therapeutic approaches including antiviral drugs, immunomodulatory therapies, and repurposed medications have been investigated to alleviate disease severity and improve patient outcomes. Through a comprehensive analysis of these scientific efforts, the review provides an overview of the advancements made in understanding and tackling SARS-CoV-2, while underscoring the need for continued research to address the evolving challenges posed by this global health crisis.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"307-325"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Silico and In vitro Analysis of Phenolic Acids for Identification of Potential DHFR Inhibitors as Antimicrobial and Anticancer Agents. 对酚酸进行硅学和体外分析,以鉴定作为抗菌剂和抗癌剂的潜在 DHFR 抑制剂。
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/1389203724666230825142558
Renu Sehrawat, Priyanka Rathee, Pooja Rathee, Sarita Khatkar, Esra Küpeli Akkol, Anurag Khatkar

Background: DHFR is an indispensable enzyme required for the survival of almost all prokaryotic and eukaryotic cells, making it an attractive molecular target for drug design.

Objective: In this study, a combined in silico and in vitro approach was utilized to screen out potential anticancer and antimicrobial agents by using DHFR PDB ID 2W9S (for antimicrobial) and 1U72 (for anticancer).

Methods: Computational work was performed using Maestro Schrodinger Glide software. The DHFR inhibitory activity of the selected compounds was assessed using the DHFR test kit (CS0340-Sigma- Aldrich).

Results: Exhaustive analysis of in silico results revealed that some natural phenolic acids have a good docking score when compared to standards, i.e., trimethoprim and methotrexate, and have astonishing interactions with crucial amino acid residues available in the binding pocket of DHFR, such as Phe 92, Asp 27, Ser 49, Asn 18, and Tyr 98. In particular, digallic acid and chlorogenic acid have amazing interactions with docking scores of -9.9 kcal/mol and -9.6 kcal/mol, respectively, for the targeted protein 2W9S. Docking scores of -10.3 kcal/mol and -10.2 kcal/mol, respectively, for targeted protein 1U72. The best hits were then tested in vitro to evaluate the DHFR inhibitory activity of the compounds. DHFR inhibition activity results are in correlation with molecular docking results.

Conclusion: In silico and in vitro results confirmed the good binding and inhibitory activity of some phenolic acids to the modeled target proteins. Among all the studied natural phenolic acids, chlorogenic acid, digallic acid, and rosmarinic acid appeared to be the most potential leads for future chemical alteration. This study can provide significant speculative guidance for the design and development of potent DHFR inhibitors in the future by using these compounds as leads.

背景:DHFR 是几乎所有原核细胞和真核细胞生存所必需的一种不可或缺的酶,使其成为药物设计的一个有吸引力的分子靶标:在本研究中,利用 DHFR PDB IDs 2W9S (抗菌)和 1U72 (抗癌),采用硅学和体外相结合的方法筛选出潜在的抗癌和抗菌药物:计算工作使用 Maestro Schrodinger Glide 软件进行。方法:使用 Maestro Schrodinger Glide 软件进行计算,使用 DHFR 检测试剂盒(CS0340-Sigma-Aldrich)评估所选化合物的 DHFR 抑制活性:详尽的内模拟结果分析表明,与三甲双胍和甲氨蝶呤等标准化合物相比,一些天然酚酸类化合物具有良好的对接得分,并与 DHFR 结合袋中的关键氨基酸残基(如 Phe 92、Asp 27、Ser 49、Asn 18 和 Tyr 98)具有惊人的相互作用。特别是,地高辛和绿原酸与目标蛋白 2W9S 的对接得分分别为-9.9 kcal/mol 和-9.6 kcal/mol,具有惊人的相互作用。目标蛋白 1U72 的对接得分分别为 -10.3 kcal/mol 和 -10.2 kcal/mol。然后对最佳命中化合物进行体外测试,以评估其 DHFR 抑制活性。DHFR 抑制活性结果与分子对接结果一致:硅学和体外实验结果证实,一些酚酸类化合物与模型中的靶蛋白具有良好的结合力和抑制活性。在所有研究的天然酚酸中,绿原酸、地高辛酸和迷迭香酸似乎是未来最有潜力进行化学改变的线索。这项研究可以为今后以这些化合物为先导设计和开发强效 DHFR 抑制剂提供重要的推测指导。
{"title":"<i>In Silico</i> and <i>In vitro</i> Analysis of Phenolic Acids for Identification of Potential DHFR Inhibitors as Antimicrobial and Anticancer Agents.","authors":"Renu Sehrawat, Priyanka Rathee, Pooja Rathee, Sarita Khatkar, Esra Küpeli Akkol, Anurag Khatkar","doi":"10.2174/1389203724666230825142558","DOIUrl":"10.2174/1389203724666230825142558","url":null,"abstract":"<p><strong>Background: </strong>DHFR is an indispensable enzyme required for the survival of almost all prokaryotic and eukaryotic cells, making it an attractive molecular target for drug design.</p><p><strong>Objective: </strong>In this study, a combined <i>in silico</i> and <i>in vitro</i> approach was utilized to screen out potential anticancer and antimicrobial agents by using DHFR PDB ID 2W9S (for antimicrobial) and 1U72 (for anticancer).</p><p><strong>Methods: </strong>Computational work was performed using Maestro Schrodinger Glide software. The DHFR inhibitory activity of the selected compounds was assessed using the DHFR test kit (CS0340-Sigma- Aldrich).</p><p><strong>Results: </strong>Exhaustive analysis of <i>in silico</i> results revealed that some natural phenolic acids have a good docking score when compared to standards, i.e., trimethoprim and methotrexate, and have astonishing interactions with crucial amino acid residues available in the binding pocket of DHFR, such as Phe 92, Asp 27, Ser 49, Asn 18, and Tyr 98. In particular, digallic acid and chlorogenic acid have amazing interactions with docking scores of -9.9 kcal/mol and -9.6 kcal/mol, respectively, for the targeted protein 2W9S. Docking scores of -10.3 kcal/mol and -10.2 kcal/mol, respectively, for targeted protein 1U72. The best hits were then tested <i>in vitro</i> to evaluate the DHFR inhibitory activity of the compounds. DHFR inhibition activity results are in correlation with molecular docking results.</p><p><strong>Conclusion: </strong><i>In silico</i> and <i>in vitro</i> results confirmed the good binding and inhibitory activity of some phenolic acids to the modeled target proteins. Among all the studied natural phenolic acids, chlorogenic acid, digallic acid, and rosmarinic acid appeared to be the most potential leads for future chemical alteration. This study can provide significant speculative guidance for the design and development of potent DHFR inhibitors in the future by using these compounds as leads.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"44-58"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10111446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification, Characterization and Evaluation of the Anticoagulant Effect of an Uncompetitive Trypsin Inhibitor obtained from Bauhinia pulchella (Benth) Seeds. 从 Bauhinia pulchella (Benth) 种子中提取的一种非竞争性胰蛋白酶抑制剂的纯化、特征描述和抗凝血效果评估。
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/1389203724666230908114115
Renato R Roma, Lucas P Dias, Ana L E Santos, Romério R S Silva, Maria H C Santos, Bruno A M Rocha, Rômulo F Carneiro, Celso S Nagano, Alexandre H Sampaio, Maria L V Oliva, Cláudio G L Silva, Racquel O S Souza, Claudener S Teixeira

Introduction: Trypsin inhibitors (TIs) have the ability to competitively or non-competitively bind to trypsin and inhibit its action. These inhibitors are commonly found in plants and are used in protease inhibition studies involved in biochemical pathways of pharmacological interest.

Objectives: This work aimed to purify a trypsin inhibitor from Bauhinia pulchella seeds (BpuTI), describing its kinetic mechanism and anticoagulant effect.

Methods: Affinity chromatography, protein assay, and SDS-PAGE were used to purify the inhibitor. Mass spectrometry, inhibition assays, and enzyme kinetics were used to characterize the inhibitor. In vitro assays were performed to verify its ability to prolong blood clotting time.

Results: Affinity chromatography on a Trypsin-Sepharose 4B column gave a yield of 43.1. BpuTI has an apparent molecular mass of 20 kDa with glycosylation (1.15%). Protein identification was determined by MS/MS, and BpuTI showed similarity to several Kunitz-type trypsin inhibitors. BpuTI inhibited bovine trypsin as an uncompetitive inhibitor with IC50 (3 x 10-6 M) and Ki (1.05 x 10-6 M). Additionally, BpuTI showed high stability to temperature and pH variations, maintaining its activity up to 100ºC and in extreme pH ranges. However, the inhibitor was susceptible to reducing agents, such as DTT, which completely abolished its activity. BpuTI showed an anticoagulant effect in vitro at a concentration of 33 μM, prolonging clotting time by 2.6 times.

Conclusion: Our results suggest that BpuTI can be a biological tool to be used in blood clotting studies.

简介:胰蛋白酶抑制剂(TIs)能够竞争性或非竞争性地与胰蛋白酶结合并抑制其作用。这些抑制剂通常存在于植物中,可用于药理生化途径中蛋白酶抑制研究:本研究旨在从紫荆种子中纯化一种胰蛋白酶抑制剂(BpuTI),描述其动力学机制和抗凝血作用:方法:采用亲和层析法、蛋白质测定法和 SDS-PAGE 法纯化抑制剂。方法:采用亲和层析法、蛋白质测定法和 SDS-PAGE 法纯化抑制剂,并利用质谱法、抑制测定法和酶动力学来描述抑制剂的特性。体外试验验证了其延长血液凝固时间的能力:在胰蛋白酶-Sepharose 4B 柱上进行亲和层析的产率为 43.1。BpuTI 的表观分子量为 20 kDa,糖基化率为 1.15%。蛋白质鉴定是通过 MS/MS 确定的,BpuTI 显示出与几种 Kunitz 型胰蛋白酶抑制剂的相似性。BpuTI 对牛胰蛋白酶的抑制作用是非竞争性的,其 IC50(3 x 10-6 M)和 Ki(1.05 x 10-6 M)。此外,BpuTI 对温度和 pH 值的变化具有很高的稳定性,在 100ºC 和极端 pH 值范围内仍能保持活性。不过,该抑制剂易受还原剂(如 DTT)的影响,后者会完全削弱其活性。BpuTI 在浓度为 33 μM 时具有体外抗凝作用,可使凝血时间延长 2.6 倍:我们的研究结果表明,BpuTI 可以作为一种生物学工具用于凝血研究。
{"title":"Purification, Characterization and Evaluation of the Anticoagulant Effect of an Uncompetitive Trypsin Inhibitor obtained from <i>Bauhinia pulchella</i> (Benth) Seeds.","authors":"Renato R Roma, Lucas P Dias, Ana L E Santos, Romério R S Silva, Maria H C Santos, Bruno A M Rocha, Rômulo F Carneiro, Celso S Nagano, Alexandre H Sampaio, Maria L V Oliva, Cláudio G L Silva, Racquel O S Souza, Claudener S Teixeira","doi":"10.2174/1389203724666230908114115","DOIUrl":"10.2174/1389203724666230908114115","url":null,"abstract":"<p><strong>Introduction: </strong>Trypsin inhibitors (TIs) have the ability to competitively or non-competitively bind to trypsin and inhibit its action. These inhibitors are commonly found in plants and are used in protease inhibition studies involved in biochemical pathways of pharmacological interest.</p><p><strong>Objectives: </strong>This work aimed to purify a trypsin inhibitor from <i>Bauhinia pulchella</i> seeds (<i>Bpu</i>TI), describing its kinetic mechanism and anticoagulant effect.</p><p><strong>Methods: </strong>Affinity chromatography, protein assay, and SDS-PAGE were used to purify the inhibitor. Mass spectrometry, inhibition assays, and enzyme kinetics were used to characterize the inhibitor. <i>In vitro</i> assays were performed to verify its ability to prolong blood clotting time.</p><p><strong>Results: </strong>Affinity chromatography on a Trypsin-Sepharose 4B column gave a yield of 43.1. <i>Bpu</i>TI has an apparent molecular mass of 20 kDa with glycosylation (1.15%). Protein identification was determined by MS/MS, and <i>Bpu</i>TI showed similarity to several Kunitz-type trypsin inhibitors. <i>Bpu</i>TI inhibited bovine trypsin as an uncompetitive inhibitor with IC50 (3 x 10<sup>-6</sup> M) and Ki (1.05 x 10<sup>-6</sup> M). Additionally, <i>Bpu</i>TI showed high stability to temperature and pH variations, maintaining its activity up to 100ºC and in extreme pH ranges. However, the inhibitor was susceptible to reducing agents, such as DTT, which completely abolished its activity. <i>Bpu</i>TI showed an anticoagulant effect <i>in vitro</i> at a concentration of 33 μM, prolonging clotting time by 2.6 times.</p><p><strong>Conclusion: </strong>Our results suggest that <i> Bpu</i>TI can be a biological tool to be used in blood clotting studies.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"172-182"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10554809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanism-based Suppression of Cancer by Targeting DNA-Replicating Enzymes. 以 DNA 复制酶为靶标的癌症抑制机制
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/1389203724666230512144011
Preeti Arya, Hitesh Malhotra, Benu Chaudhary, Amrit Sarwara, Rajat Goyal, Chunpeng Wan, Dinesh Kumar Mishra, Rupesh Kumar Gautam

The human genetic structure undergoes continuous wear and tear process due to the mere presence of extrinsic as well as intrinsic factors. In normal physiological cells, DNA damage initiates various checkpoints that may activate the repair system or induce apoptosis that helps maintain cellular integrity. While in cancerous cells, due to alterations in signaling pathways and defective checkpoints, there exists a marked deviation of error-free DNA repairing/synthesis. Currently, cancer therapy targeting the DNA damage response shows significant therapeutic potential by tailoring the therapy from non-specific to tumor-specific activity. Recently, numerous drugs that target the DNA replicating enzymes have been approved or some are under clinical trial. Drugs like PARP and PARG inhibitors showed sweeping effects against cancer cells. This review highlights the mechanistic study of different drug categories that target DNA replication and thus depicts the futuristic approach of targeted therapy.

仅仅由于外在和内在因素的存在,人类基因结构就经历了持续的磨损过程。在正常的生理细胞中,DNA 损伤会启动各种检查点,从而激活修复系统或诱导细胞凋亡,帮助维持细胞的完整性。而在癌细胞中,由于信号通路的改变和检查点的缺陷,存在着明显的无差错 DNA 修复/合成偏差。目前,以 DNA 损伤反应为靶点的癌症疗法具有显著的治疗潜力,可将非特异性疗法调整为肿瘤特异性疗法。最近,许多针对 DNA 复制酶的药物已经获得批准,或正在进行临床试验。PARP和PARG抑制剂等药物对癌细胞产生了巨大的作用。这篇综述重点介绍了针对 DNA 复制的不同药物类别的机理研究,从而描绘了靶向治疗的未来发展方向。
{"title":"Mechanism-based Suppression of Cancer by Targeting DNA-Replicating Enzymes.","authors":"Preeti Arya, Hitesh Malhotra, Benu Chaudhary, Amrit Sarwara, Rajat Goyal, Chunpeng Wan, Dinesh Kumar Mishra, Rupesh Kumar Gautam","doi":"10.2174/1389203724666230512144011","DOIUrl":"10.2174/1389203724666230512144011","url":null,"abstract":"<p><p>The human genetic structure undergoes continuous wear and tear process due to the mere presence of extrinsic as well as intrinsic factors. In normal physiological cells, DNA damage initiates various checkpoints that may activate the repair system or induce apoptosis that helps maintain cellular integrity. While in cancerous cells, due to alterations in signaling pathways and defective checkpoints, there exists a marked deviation of error-free DNA repairing/synthesis. Currently, cancer therapy targeting the DNA damage response shows significant therapeutic potential by tailoring the therapy from non-specific to tumor-specific activity. Recently, numerous drugs that target the DNA replicating enzymes have been approved or some are under clinical trial. Drugs like PARP and PARG inhibitors showed sweeping effects against cancer cells. This review highlights the mechanistic study of different drug categories that target DNA replication and thus depicts the futuristic approach of targeted therapy.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"4-11"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advancements of the CRISPR/Cas9 System in the Treatment of Liver Cancer. CRISPR/Cas9系统在癌症治疗中的进展。
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/0113892037238265231006051215
Zhuoyu Li, Ziming Han

In recent years, the CRISPR/Cas9 system has become a rapidly advancing gene editing technology with significant advantages in various fields, particularly biomedicine. Liver cancer is a severe malignancy that threatens human health and is primarily treated with surgery, radiotherapy, and chemotherapy. However, surgery may not be suitable for advanced cases of liver cancer with distant metastases. Moreover, radiotherapy and chemotherapy have low specificity and numerous side effects that limit their effectiveness; therefore, more effective and safer treatments are required. With the advancement of the biomolecular mechanism of cancer, CRISPR/Cas9 gene editing technology has been widely used in the study of liver cancer to gain insights into gene functions, establish tumor models, screen tumor phenotype-related genes, and perform gene therapy. This review outlines the research progress of CRISPR/Cas9 gene editing technology in the treatment of liver cancer and provides a relevant theoretical basis for its research and application in the treatment of liver cancer.

近年来,CRISPR/Cas9系统已成为一种快速发展的基因编辑技术,在各个领域,特别是生物医学领域具有显著优势。癌症是一种严重威胁人类健康的恶性肿瘤,主要通过手术、放疗和化疗进行治疗。然而,手术可能不适合晚期癌症伴远处转移的病例。此外,放疗和化疗的特异性低,副作用多,限制了其有效性;因此,需要更有效、更安全的治疗方法。随着癌症生物分子机制的进步,CRISPR/Cas9基因编辑技术已广泛应用于癌症的研究,以深入了解基因功能,建立肿瘤模型,筛选肿瘤表型相关基因,进行基因治疗。综述了CRISPR/Cas9基因编辑技术在治疗癌症中的研究进展,为其在癌症治疗中的研究和应用提供了相关的理论依据。
{"title":"Advancements of the CRISPR/Cas9 System in the Treatment of Liver Cancer.","authors":"Zhuoyu Li, Ziming Han","doi":"10.2174/0113892037238265231006051215","DOIUrl":"10.2174/0113892037238265231006051215","url":null,"abstract":"<p><p>In recent years, the CRISPR/Cas9 system has become a rapidly advancing gene editing technology with significant advantages in various fields, particularly biomedicine. Liver cancer is a severe malignancy that threatens human health and is primarily treated with surgery, radiotherapy, and chemotherapy. However, surgery may not be suitable for advanced cases of liver cancer with distant metastases. Moreover, radiotherapy and chemotherapy have low specificity and numerous side effects that limit their effectiveness; therefore, more effective and safer treatments are required. With the advancement of the biomolecular mechanism of cancer, CRISPR/Cas9 gene editing technology has been widely used in the study of liver cancer to gain insights into gene functions, establish tumor models, screen tumor phenotype-related genes, and perform gene therapy. This review outlines the research progress of CRISPR/Cas9 gene editing technology in the treatment of liver cancer and provides a relevant theoretical basis for its research and application in the treatment of liver cancer.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"154-162"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41233097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diabetic Wound: Pathophysiology, Complications and Treatment Strategies. 糖尿病伤口:病理生理学,并发症和治疗策略。
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/0113892037276171231016103320
Sunita Chauhan, Monika Gulia, Rahul Pratap Singh, Vikas Jhawat

Diabetic wound healing is expected to affect 25% of all diabetics, resulting in less severe external factors, economic costs, and less trauma. Topical formulations have been continually improved to achieve a range of amazing properties and have had a significant impact on the management of diabetic wounds. Topical insulin has become one of the most attractive and convenient wound healing techniques due to its excellent biocompatibility, water retention, and therapeutic properties. Multiple versatile topical insulins have been identified and have shown promise over the past few years as they greatly facilitate the management of diabetic wounds as we understand their etiology. The physiological wound healing process repairs damaged tissue and restores skin integrity. For about a century, insulin, a powerful healing agent, and it has been utilized in several clinical and experimental researches research studies to accelerate the healing of various injuries.

糖尿病伤口愈合预计将影响25%的糖尿病患者,从而减少严重的外部因素、经济成本和创伤。局部配方不断改进,达到了一系列惊人的性能,并对糖尿病伤口的管理产生了重大影响。局部胰岛素由于其优异的生物相容性、保水性和治疗性能,已成为最具吸引力和最方便的伤口愈合技术之一。在过去的几年里,多种多功能局部胰岛素已经被发现,并显示出了前景,因为随着我们对其病因的了解,它们极大地促进了糖尿病伤口的治疗。生理伤口愈合过程修复受损组织,恢复皮肤完整性。大约一个世纪以来,胰岛素作为一种强大的愈合剂,已被用于多项临床和实验研究研究,以加速各种损伤的愈合。
{"title":"Diabetic Wound: Pathophysiology, Complications and Treatment Strategies.","authors":"Sunita Chauhan, Monika Gulia, Rahul Pratap Singh, Vikas Jhawat","doi":"10.2174/0113892037276171231016103320","DOIUrl":"10.2174/0113892037276171231016103320","url":null,"abstract":"<p><p>Diabetic wound healing is expected to affect 25% of all diabetics, resulting in less severe external factors, economic costs, and less trauma. Topical formulations have been continually improved to achieve a range of amazing properties and have had a significant impact on the management of diabetic wounds. Topical insulin has become one of the most attractive and convenient wound healing techniques due to its excellent biocompatibility, water retention, and therapeutic properties. Multiple versatile topical insulins have been identified and have shown promise over the past few years as they greatly facilitate the management of diabetic wounds as we understand their etiology. The physiological wound healing process repairs damaged tissue and restores skin integrity. For about a century, insulin, a powerful healing agent, and it has been utilized in several clinical and experimental researches research studies to accelerate the healing of various injuries.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"200-205"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71421455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neurokinin B Administration Induces Dose Dependent Proliferation of Seminal Vesicles in Adult Rats. 神经激肽 B 给药诱导成年大鼠精囊的剂量依赖性增殖
IF 1.9 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/0113892037264538231128072614
Muhammad Haris Ramzan, Mohsin Shah, Faiqah Ramzan

Background: Neurokinin B; an endogenous decapeptide, mediates its reproductive physiological actions through gonadotropin releasing hormone. Despite the potential role of Neurokinin B on seminal vesicles, its effects on seminal vesicles in adult male mammals remain elusive. We aimed to investigate the potentials of variable doses of Neurokinin B, its agonist and antagonist on histomorphology and expression of NK3R on seminal vesicles, and secretory activity of seminal vesicles in adult male rats.

Methods: Adult male Sprague Dawley rats (n=10 in each group) were administered intraperitoneally with Neurokinin B in three variable doses: 1 μg, 1 ηg and 10 ρg while, Senktide (Neurokinin B agonist) and SB222200 (Neurokinin B antagonist) in 1 μg doses consecutively for 12 days. After 12 days of peptide treatment, half of the animals (n=05) in each group were sacrificed while remaining half (n=05) were kept for another 12 days without any treatment to investigate treatment reversal. Seminal vesicles were dissected and excised tissue was processed for light microscopy, immunohistochemistry and estimation of seminal fructose levels.

Results: Treatment with Neurokinin B and Senktide significantly increased while SB222200 slightly decrease the seminal vesicles weight, epithelial height and seminal fructose levels as compared to control. Light microscopy revealed increased epithelial height and epithelial folding as compared to control in all Neurokinin B and Senktide treated groups while decreased in SB222200. Effects of various doses of Neurokinin B, Senktide and SB222200 on seminal vesicles weight, epithelial height, seminal fructose levels and histomorphology were reversed when rats were maintained without treatments. Immuno-expression of Neurokinin B shows no change in treatment and reversal groups.

Conclusion: Continuous administration of Neurokinin B and Senktide effect positively while SB222200 have detrimental effects on cellular morphology, epithelial height and seminal fructose levels in seminal vesicles. Effects of peptide treatments depicted a reversal towards control group when rats were kept without any treatment.

背景:神经激肽 B 是一种内源性十肽,通过促性腺激素释放激素介导其生殖生理作用。尽管神经激肽B对精囊具有潜在作用,但其对成年雄性哺乳动物精囊的影响仍然难以捉摸。我们旨在研究不同剂量的神经激肽B、其激动剂和拮抗剂对成年雄性大鼠精囊组织形态学、精囊上NK3R的表达以及精囊分泌活性的潜在影响:成年雄性 Sprague Dawley 大鼠(每组 10 只)腹腔注射三种不同剂量的神经激肽 B:1 μg、1 ηg和10 μg,同时连续注射1 μg剂量的Senktide(神经激肽B激动剂)和SB222200(神经激肽B拮抗剂)12天。多肽治疗 12 天后,每组一半动物(n=05)被处死,另一半动物(n=05)在没有任何治疗的情况下再保留 12 天,以研究治疗逆转。解剖精囊并对切除的组织进行光镜观察、免疫组化和精液果糖水平评估:结果:与对照组相比,神经激肽B和Senktide能明显增加精囊重量、上皮高度和精液果糖水平,而SB222200能略微降低精囊重量、上皮高度和精液果糖水平。光镜观察显示,与对照组相比,所有神经激肽 B 和参肽处理组的上皮高度和上皮褶皱均有所增加,而 SB222200 处理组则有所减少。不同剂量的神经激肽 B、Senktide 和 SB222200 对精囊重量、上皮高度、精液果糖水平和组织形态学的影响在大鼠未接受治疗的情况下被逆转。神经激肽 B 的免疫表达在治疗组和逆转组均无变化:连续给药神经激肽 B 和 SB222200 会对精囊细胞形态、上皮高度和精液果糖水平产生积极影响,而 SB222200 则会产生有害影响。当大鼠未接受任何治疗时,肽治疗的效果会向对照组逆转。
{"title":"Neurokinin B Administration Induces Dose Dependent Proliferation of Seminal Vesicles in Adult Rats.","authors":"Muhammad Haris Ramzan, Mohsin Shah, Faiqah Ramzan","doi":"10.2174/0113892037264538231128072614","DOIUrl":"10.2174/0113892037264538231128072614","url":null,"abstract":"<p><strong>Background: </strong>Neurokinin B; an endogenous decapeptide, mediates its reproductive physiological actions through gonadotropin releasing hormone. Despite the potential role of Neurokinin B on seminal vesicles, its effects on seminal vesicles in adult male mammals remain elusive. We aimed to investigate the potentials of variable doses of Neurokinin B, its agonist and antagonist on histomorphology and expression of NK3R on seminal vesicles, and secretory activity of seminal vesicles in adult male rats.</p><p><strong>Methods: </strong>Adult male Sprague Dawley rats (n=10 in each group) were administered intraperitoneally with Neurokinin B in three variable doses: 1 μg, 1 ηg and 10 ρg while, Senktide (Neurokinin B agonist) and SB222200 (Neurokinin B antagonist) in 1 μg doses consecutively for 12 days. After 12 days of peptide treatment, half of the animals (n=05) in each group were sacrificed while remaining half (n=05) were kept for another 12 days without any treatment to investigate treatment reversal. Seminal vesicles were dissected and excised tissue was processed for light microscopy, immunohistochemistry and estimation of seminal fructose levels.</p><p><strong>Results: </strong>Treatment with Neurokinin B and Senktide significantly increased while SB222200 slightly decrease the seminal vesicles weight, epithelial height and seminal fructose levels as compared to control. Light microscopy revealed increased epithelial height and epithelial folding as compared to control in all Neurokinin B and Senktide treated groups while decreased in SB222200. Effects of various doses of Neurokinin B, Senktide and SB222200 on seminal vesicles weight, epithelial height, seminal fructose levels and histomorphology were reversed when rats were maintained without treatments. Immuno-expression of Neurokinin B shows no change in treatment and reversal groups.</p><p><strong>Conclusion: </strong>Continuous administration of Neurokinin B and Senktide effect positively while SB222200 have detrimental effects on cellular morphology, epithelial height and seminal fructose levels in seminal vesicles. Effects of peptide treatments depicted a reversal towards control group when rats were kept without any treatment.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"339-352"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139511251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioconjugation Techniques for Enhancing Stability and Targeting Efficiency of Protein and Peptide Therapeutics. 提高蛋白质和肽治疗学稳定性和靶向效率的生物偶联技术。
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/0113892037268777231013154850
Tanuja Bisht, Anupriya Adhikari, Shivanand Patil, Shivang Dhoundiyal

Bioconjugation techniques have emerged as powerful tools for enhancing the stability and targeting efficiency of protein and peptide therapeutics. This review provides a comprehensive analysis of the various bioconjugation strategies employed in the field. The introduction highlights the significance of bioconjugation techniques in addressing stability and targeting challenges associated with protein and peptide-based drugs. Chemical and enzymatic bioconjugation methods are discussed, along with crosslinking strategies for covalent attachment and site-specific conjugation approaches. The role of bioconjugation in improving stability profiles is explored, showcasing case studies that demonstrate successful stability enhancement. Furthermore, bioconjugation techniques for ligand attachment and targeting are presented, accompanied by examples of targeted protein and peptide therapeutics. The review also covers bioconjugation approaches for prolonging circulation and controlled release, focusing on strategies to extend half-life, reduce clearance, and design-controlled release systems. Analytical characterization techniques for bioconjugates, including the evaluation of conjugation efficiency, stability, and assessment of biological activity and targeting efficiency, are thoroughly examined. In vivo considerations and clinical applications of bioconjugated protein and peptide therapeutics, including pharmacokinetic and pharmacodynamic considerations, as well as preclinical and clinical developments, are discussed. Finally, the review concludes with an overview of future perspectives, emphasizing the potential for novel conjugation methods and advanced targeting strategies to further enhance the stability and targeting efficiency of protein and peptide therapeutics.

生物偶联技术已成为增强蛋白质和肽治疗的稳定性和靶向效率的强大工具。这篇综述对该领域采用的各种生物偶联策略进行了全面分析。引言强调了生物偶联技术在解决蛋白质和肽类药物的稳定性和靶向挑战方面的重要性。讨论了化学和酶生物偶联方法,以及共价连接的交联策略和位点特异性偶联方法。探讨了生物偶联在改善稳定性方面的作用,展示了成功增强稳定性的案例研究。此外,还介绍了用于配体附着和靶向的生物偶联技术,以及靶向蛋白质和肽治疗的实例。该综述还涵盖了延长循环和控制释放的生物偶联方法,重点是延长半衰期、减少清除和设计控制释放系统的策略。生物偶联物的分析表征技术,包括偶联效率、稳定性的评估,以及生物活性和靶向效率的评估,都得到了彻底的检查。讨论了生物偶联蛋白质和肽疗法的体内考虑因素和临床应用,包括药代动力学和药效学考虑因素,以及临床前和临床发展。最后,综述总结了未来的前景,强调了新的偶联方法和先进的靶向策略的潜力,以进一步提高蛋白质和肽治疗的稳定性和靶向效率。
{"title":"Bioconjugation Techniques for Enhancing Stability and Targeting Efficiency of Protein and Peptide Therapeutics.","authors":"Tanuja Bisht, Anupriya Adhikari, Shivanand Patil, Shivang Dhoundiyal","doi":"10.2174/0113892037268777231013154850","DOIUrl":"10.2174/0113892037268777231013154850","url":null,"abstract":"<p><p>Bioconjugation techniques have emerged as powerful tools for enhancing the stability and targeting efficiency of protein and peptide therapeutics. This review provides a comprehensive analysis of the various bioconjugation strategies employed in the field. The introduction highlights the significance of bioconjugation techniques in addressing stability and targeting challenges associated with protein and peptide-based drugs. Chemical and enzymatic bioconjugation methods are discussed, along with crosslinking strategies for covalent attachment and site-specific conjugation approaches. The role of bioconjugation in improving stability profiles is explored, showcasing case studies that demonstrate successful stability enhancement. Furthermore, bioconjugation techniques for ligand attachment and targeting are presented, accompanied by examples of targeted protein and peptide therapeutics. The review also covers bioconjugation approaches for prolonging circulation and controlled release, focusing on strategies to extend half-life, reduce clearance, and design-controlled release systems. Analytical characterization techniques for bioconjugates, including the evaluation of conjugation efficiency, stability, and assessment of biological activity and targeting efficiency, are thoroughly examined. <i>In vivo</i> considerations and clinical applications of bioconjugated protein and peptide therapeutics, including pharmacokinetic and pharmacodynamic considerations, as well as preclinical and clinical developments, are discussed. Finally, the review concludes with an overview of future perspectives, emphasizing the potential for novel conjugation methods and advanced targeting strategies to further enhance the stability and targeting efficiency of protein and peptide therapeutics.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"226-243"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71421552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ARL15 and its Multiple Disease Association: Emerging Functions and Potential Therapeutic Application. ARL15 及其与多种疾病的关联:新功能和潜在治疗应用
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/1389203724666230915123217
Manisha Saini, Varnita Anand, Aditya Sharma, Anuj Pandey, Bittianda Kuttapa Thelma, Suman Kundu

ARL15 is a member of the RAS superfamily of small GTPases and is associated with several metabolic traits, including increased risk of diabetes, rheumatoid arthritis and lipid metabolism disorders. The ARL15 gene encodes for an uncharacterized small GTP binding protein. Its precise role in human physiology remains unknown, but several genetic association studies have recognized different variants in this gene to be statistically associated with numerous traits and complex diseases. Here, we provided the unique features of ARL15 small G protein, its association with varied metabolic and lifestyle diseases, its function in vesicular and lipid trafficking, and its binding partners. We outlined this protein as a promising and emerging therapeutic target to combat metabolic disorders like cardiovascular diseases, diabetes and rheumatoid arthritis. The review provides a comprehensive description of the current advancements in ARL15 research with a perspective that focused research will position this small GTPase as a viable target for the treatment of rheumatoid arthritis.

ARL15 是小 GTP 酶 RAS 超家族的成员,与多种代谢特征有关,包括糖尿病、类风湿性关节炎和脂质代谢紊乱风险的增加。ARL15 基因编码一种未定性的小 GTP 结合蛋白。它在人类生理学中的确切作用尚不清楚,但几项遗传关联研究已确认该基因的不同变异与许多性状和复杂疾病有统计学关联。在此,我们介绍了 ARL15 小 G 蛋白的独特特征、它与各种代谢疾病和生活方式疾病的关联、它在囊泡和脂质贩运中的功能及其结合伙伴。我们概述了该蛋白是一种很有前景的新兴治疗靶点,可用于防治心血管疾病、糖尿病和类风湿性关节炎等代谢性疾病。这篇综述全面介绍了 ARL15 研究的最新进展,并指出重点研究将使这种小 GTPase 成为治疗类风湿性关节炎的可行靶点。
{"title":"ARL15 and its Multiple Disease Association: Emerging Functions and Potential Therapeutic Application.","authors":"Manisha Saini, Varnita Anand, Aditya Sharma, Anuj Pandey, Bittianda Kuttapa Thelma, Suman Kundu","doi":"10.2174/1389203724666230915123217","DOIUrl":"10.2174/1389203724666230915123217","url":null,"abstract":"<p><p>ARL15 is a member of the RAS superfamily of small GTPases and is associated with several metabolic traits, including increased risk of diabetes, rheumatoid arthritis and lipid metabolism disorders. The ARL15 gene encodes for an uncharacterized small GTP binding protein. Its precise role in human physiology remains unknown, but several genetic association studies have recognized different variants in this gene to be statistically associated with numerous traits and complex diseases. Here, we provided the unique features of ARL15 small G protein, its association with varied metabolic and lifestyle diseases, its function in vesicular and lipid trafficking, and its binding partners. We outlined this protein as a promising and emerging therapeutic target to combat metabolic disorders like cardiovascular diseases, diabetes and rheumatoid arthritis. The review provides a comprehensive description of the current advancements in ARL15 research with a perspective that focused research will position this small GTPase as a viable target for the treatment of rheumatoid arthritis.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"137-153"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10286952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soluble Factors Associated with Denervation-induced Skeletal Muscle Atrophy. 与去神经支配诱导的骨骼肌萎缩相关的可溶性因子。
IF 2.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.2174/0113892037189827231018092036
Marianny Portal Rodríguez, Claudio Cabello-Verrugio

Skeletal muscle tissue has the critical function of mechanical support protecting the body. In addition, its functions are strongly influenced by the balanced synthesis and degradation processes of structural and regulatory proteins. The inhibition of protein synthesis and/or the activation of catabolism generally determines a pathological state or condition called muscle atrophy, a reduction in muscle mass that results in partial or total loss of function. It has been established that many pathophysiological conditions can cause a decrease in muscle mass. Skeletal muscle innervation involves stable and functional neural interactions with muscles via neuromuscular junctions and is essential for maintaining normal muscle structure and function. Loss of motor innervation induces rapid skeletal muscle fiber degeneration with activation of atrophy-related signaling and subsequent disassembly of sarcomeres, altering normal muscle function. After denervation, an inflammation stage is characterized by the increased expression of pro-inflammatory cytokines that determine muscle atrophy. In this review, we highlighted the impact of some soluble factors on the development of muscle atrophy by denervation.

骨骼肌组织具有保护人体的关键机械支撑功能。此外,其功能还受到结构蛋白和调节蛋白的平衡合成和降解过程的强烈影响。蛋白质合成的抑制和/或分解代谢的激活通常决定了一种称为肌肉萎缩的病理状态或状况,肌肉质量的减少导致部分或全部功能丧失。已经确定,许多病理生理条件可导致肌肉量减少。骨骼肌神经支配通过神经肌肉连接与肌肉进行稳定和功能性的神经相互作用,对维持正常的肌肉结构和功能至关重要。运动神经支配的丧失导致骨骼肌纤维快速变性,伴有萎缩相关信号的激活和随后的肌节分解,改变正常的肌肉功能。在去神经支配后,炎症阶段的特征是决定肌肉萎缩的促炎细胞因子的表达增加。在这篇综述中,我们强调了一些可溶性因子在去神经支配肌萎缩发展中的影响。
{"title":"Soluble Factors Associated with Denervation-induced Skeletal Muscle Atrophy.","authors":"Marianny Portal Rodríguez, Claudio Cabello-Verrugio","doi":"10.2174/0113892037189827231018092036","DOIUrl":"10.2174/0113892037189827231018092036","url":null,"abstract":"<p><p>Skeletal muscle tissue has the critical function of mechanical support protecting the body. In addition, its functions are strongly influenced by the balanced synthesis and degradation processes of structural and regulatory proteins. The inhibition of protein synthesis and/or the activation of catabolism generally determines a pathological state or condition called muscle atrophy, a reduction in muscle mass that results in partial or total loss of function. It has been established that many pathophysiological conditions can cause a decrease in muscle mass. Skeletal muscle innervation involves stable and functional neural interactions with muscles <i>via</i> neuromuscular junctions and is essential for maintaining normal muscle structure and function. Loss of motor innervation induces rapid skeletal muscle fiber degeneration with activation of atrophy-related signaling and subsequent disassembly of sarcomeres, altering normal muscle function. After denervation, an inflammation stage is characterized by the increased expression of pro-inflammatory cytokines that determine muscle atrophy. In this review, we highlighted the impact of some soluble factors on the development of muscle atrophy by denervation.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"189-199"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Current protein & peptide science
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1