Heat-cured polymethyl methacrylate is (PMMA) is the most common denture base material and some mechanical properties such as flexural strength and impact resistance etc. may lead to fail, besides its excellent properties. Polypropylene (PP) hernia mesh is a commercially available medical textile used in surgical repair of different types of hernia. It was aimed to investigate the effect of two different knitting patterns of PP meshes on the flexural properties of the PMMA denture base resin when they are used as reinforcement and compare with glass fiber mesh reinforced and no mesh used resins. Knitting patterns of the PP mesh structures were hexagonal honeycomb pattern and square pattern and these were used as single or double layer as reinforcement. 6 groups were established in the study: control group with no mesh and 5 reinforced groups with meshes (glass fiber mesh, single layer honeycomb patterned PP mesh, double layer honeycomb patterned PP mesh, single layer square patterned PP mesh, double layer square patterned PP mesh). A total of 60 samples with the dimensions of 65x10x3 mm, n=10 in each group were fabricated. The flexural strength, maximum deformation, and flexural modulus were determined by three-point bending test. Fracture surfaces were evaluated by scanning electron microscopy. The obtained data were statistically analyzed by two-way ANOVA test with Bonferroni corrections. The single layer square patterned PP mesh group exhibited the highest (76.67 ± 7.64 MPa), and the control group showed the lowest (63.49 ± 7.18 MPa) flexural strength values. The single layer glass fiber mesh group showed the highest (7.13 ± 0.55 mm) and the control group showed the lowest (4.72 ± 0.81 mm) maximum deformation values. The single layer glass fiber mesh group exhibited the highest (2131.87 ± 205.76 MPa), and the control group exhibited the lowest (1582.26 ± 98.63 MPa) flexural modulus values. Significant increase in flexural strength was observed in all polypropylene mesh-reinforced groups compared to the control group except double layer honeycomb patterned PP mesh group (p < 0.05). Using PP fiber mesh for reinforcement provide a very favorable aesthetic view and PP fiber mesh is concluded to be a promising material for reinforcement of heat-cured PMMA denture base resins.
{"title":"Effect of Knitting Pattern of PP Mesh on the Flexural Properties of Heat-cured PMMA Denture Base Resin","authors":"YERLİYURT, Kaan\u0000 , EĞRİ, Sinan\u0000 ","doi":"10.17776/csj.1184249","DOIUrl":"https://doi.org/10.17776/csj.1184249","url":null,"abstract":"Heat-cured polymethyl methacrylate is (PMMA) is the most common denture base material and some mechanical properties such as flexural strength and impact resistance etc. may lead to fail, besides its excellent properties. Polypropylene (PP) hernia mesh is a commercially available medical textile used in surgical repair of different types of hernia. It was aimed to investigate the effect of two different knitting patterns of PP meshes on the flexural properties of the PMMA denture base resin when they are used as reinforcement and compare with glass fiber mesh reinforced and no mesh used resins. Knitting patterns of the PP mesh structures were hexagonal honeycomb pattern and square pattern and these were used as single or double layer as reinforcement. 6 groups were established in the study: control group with no mesh and 5 reinforced groups with meshes (glass fiber mesh, single layer honeycomb patterned PP mesh, double layer honeycomb patterned PP mesh, single layer square patterned PP mesh, double layer square patterned PP mesh). A total of 60 samples with the dimensions of 65x10x3 mm, n=10 in each group were fabricated. The flexural strength, maximum deformation, and flexural modulus were determined by three-point bending test. Fracture surfaces were evaluated by scanning electron microscopy. The obtained data were statistically analyzed by two-way ANOVA test with Bonferroni corrections. The single layer square patterned PP mesh group exhibited the highest (76.67 ± 7.64 MPa), and the control group showed the lowest (63.49 ± 7.18 MPa) flexural strength values. The single layer glass fiber mesh group showed the highest (7.13 ± 0.55 mm) and the control group showed the lowest (4.72 ± 0.81 mm) maximum deformation values. The single layer glass fiber mesh group exhibited the highest (2131.87 ± 205.76 MPa), and the control group exhibited the lowest (1582.26 ± 98.63 MPa) flexural modulus values. Significant increase in flexural strength was observed in all polypropylene mesh-reinforced groups compared to the control group except double layer honeycomb patterned PP mesh group (p &lt; 0.05). Using PP fiber mesh for reinforcement provide a very favorable aesthetic view and PP fiber mesh is concluded to be a promising material for reinforcement of heat-cured PMMA denture base resins.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135133137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study, the electroanalytical behaviour of clopidogrel was investigated by cyclic voltammetry method. The procedure was based on clopidogrel being electrochemically oxidized at a platinum electrode in nonaqueous solutions. At 1.93 V, the oxidation peak was noted. It was discovered that clopidogrel's oxidation was diffusion-controlled. Additionally, a quick and easy SWV approach was developed and validated in this work to determine clopidogrel in pharmaceutical preparations. At concentrations between 5 and 50 µg/mL, the calibration curve was linear. The precision was given by relative standard deviation and was less than 2.73%. Accuracy was given with relative error and did not exceed 3.89%. In pharmaceutical preparations, clopidogrel had an average recovery of 100.1%. Under the chosen experimental conditions, no interference was found. The suggested method is extremely accurate and precise. Therefore, the method is applicable to the measurement of clopidogrel in pharmaceutical formulations.
{"title":"Electrochemical Study of Clopidogrel and its Determination in Pharmaceutical Preparations Using Square Wave Voltammetry","authors":"YILMAZ, Bilal\u0000 , YILMAZ, Semih\u0000 ","doi":"10.17776/csj.1282586","DOIUrl":"https://doi.org/10.17776/csj.1282586","url":null,"abstract":"In the present study, the electroanalytical behaviour of clopidogrel was investigated by cyclic voltammetry method. The procedure was based on clopidogrel being electrochemically oxidized at a platinum electrode in nonaqueous solutions. At 1.93 V, the oxidation peak was noted. It was discovered that clopidogrel's oxidation was diffusion-controlled. Additionally, a quick and easy SWV approach was developed and validated in this work to determine clopidogrel in pharmaceutical preparations. At concentrations between 5 and 50 µg/mL, the calibration curve was linear. The precision was given by relative standard deviation and was less than 2.73%. Accuracy was given with relative error and did not exceed 3.89%. In pharmaceutical preparations, clopidogrel had an average recovery of 100.1%. Under the chosen experimental conditions, no interference was found. The suggested method is extremely accurate and precise. Therefore, the method is applicable to the measurement of clopidogrel in pharmaceutical formulations.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135133257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this paper, we construct a generalisation of Ostrowski’s type inequalities with the help of new identity. By using this identity, we construct further results for ģ^'∈L^1 [c ̇,d ̆ ],ģ^'∈L^2 [c ̇,d ̆ ],ģ^''∈L^2 [c ̇,d ̆ ]. To prove our main and related results, we utilized some famous inequalities such as Gruss-inequality, Diaz-Mıtcaf’s inequality and Cauchy’s inequality. To prove our main results, we used a new multistep kernel (9-step linear kernel). Some related results are also discussed. In the end, we apply our results to numerical integration also.
{"title":"Construction of New Ostrowski’s Type Inequalities By Using Multistep Linear Kernel","authors":"QAYYUM, Yasır\u0000 , ALİ, Haider\u0000 , RASOOL, Faiz\u0000 , QAYYUM, Ather\u0000 ","doi":"10.17776/csj.1145020","DOIUrl":"https://doi.org/10.17776/csj.1145020","url":null,"abstract":"In this paper, we construct a generalisation of Ostrowski’s type inequalities with the help of new identity. By using this identity, we construct further results for ģ^'∈L^1 [c ̇,d ̆ ],ģ^'∈L^2 [c ̇,d ̆ ],ģ^''∈L^2 [c ̇,d ̆ ]. To prove our main and related results, we utilized some famous inequalities such as Gruss-inequality, Diaz-Mıtcaf’s inequality and Cauchy’s inequality. To prove our main results, we used a new multistep kernel (9-step linear kernel). Some related results are also discussed. In the end, we apply our results to numerical integration also.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135133265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this manuscript, the maximum likelihood estimators and Bayes estimators for the parameters of the modified Frechet–exponential distribution. Because the Bayes estimators cannot be obtained in closed forms, the approximate Bayes estimators are computed using the idea of Lindley’s approximation method under squared-error loss function. Then, the approximate Bayes estimates are compared with the maximum likelihood estimates in terms of mean square error and bias values using Monte Carlo simulation. Finally, real data sets belonging to COVID-19 death cases in Europe and China to are used to demonstrate the emprical results belonging to the approximate Bayes estimates, the maximum likelihood estimates.
{"title":"Bayesian Analysis for the Modified Frechet–Exponential Distribution with Covid-19 Application","authors":"AKDAM, Neriman\u0000 ","doi":"10.17776/csj.1320712","DOIUrl":"https://doi.org/10.17776/csj.1320712","url":null,"abstract":"In this manuscript, the maximum likelihood estimators and Bayes estimators for the parameters of the modified Frechet–exponential distribution. Because the Bayes estimators cannot be obtained in closed forms, the approximate Bayes estimators are computed using the idea of Lindley’s approximation method under squared-error loss function. Then, the approximate Bayes estimates are compared with the maximum likelihood estimates in terms of mean square error and bias values using Monte Carlo simulation. Finally, real data sets belonging to COVID-19 death cases in Europe and China to are used to demonstrate the emprical results belonging to the approximate Bayes estimates, the maximum likelihood estimates.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135133273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The linear structure of the Lorentz-Minkowski plane is almost the same as Euclidean plane. But, there is one different aspect. These planes have different distance functions. So, it can be interesting to study the Lorentz analogues of topics that include the distance concept in the Euclidean plane. Thus, in this study, we show that the relationship between Euclidean and Lorentz distances is given depending on the slope of the line segment. Following, we investigate Lorentz analogues of Thales’ theorem, Angle Bisector theorems, Menelaus’ theorem and Ceva’s theorem.
{"title":"On Directed Length Ratios in the Lorentz-Minkowski Plane","authors":"Abdulaziz Açikgöz","doi":"10.17776/csj.1133780","DOIUrl":"https://doi.org/10.17776/csj.1133780","url":null,"abstract":"The linear structure of the Lorentz-Minkowski plane is almost the same as Euclidean plane. But, there is one different aspect. These planes have different distance functions. So, it can be interesting to study the Lorentz analogues of topics that include the distance concept in the Euclidean plane. Thus, in this study, we show that the relationship between Euclidean and Lorentz distances is given depending on the slope of the line segment. Following, we investigate Lorentz analogues of Thales’ theorem, Angle Bisector theorems, Menelaus’ theorem and Ceva’s theorem.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this paper, we introduce a parametric extension of a certain family of summation-integral type operators on the interval [0,∞). Firstly, we obtain test functions and central moments. Secondly, we investigate weighted approximation properties for these operators and estimate the rate of convergence. Then, we give a pointwise approximation for the Peetre K-functional and functions of the Lipschitz class. Moreover, we demonstrate Voronovskaja type theorem for the operators. Finally, the convergence properties of operators to some functions are illustrated by graphics.
{"title":"Parametric Extension of a Certain Family of Summation-Integral Type Operators","authors":"Nadire Fulda Odabaşi, I. Yüksel","doi":"10.17776/csj.1173496","DOIUrl":"https://doi.org/10.17776/csj.1173496","url":null,"abstract":"In this paper, we introduce a parametric extension of a certain family of summation-integral type operators on the interval [0,∞). Firstly, we obtain test functions and central moments. Secondly, we investigate weighted approximation properties for these operators and estimate the rate of convergence. Then, we give a pointwise approximation for the Peetre K-functional and functions of the Lipschitz class. Moreover, we demonstrate Voronovskaja type theorem for the operators. Finally, the convergence properties of operators to some functions are illustrated by graphics.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45106899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, it was aimed to develop a nano drug system that can be used in passive targeting in pancreatic cancer treatment. Hydroxyapatite nanocrystals (n-HAP) produced by hydrothermal process and graphene oxide (GO) produced by hummers method were used to increase the carrier capacity of the nano drug system and to activate the drug release kinetics and drug loading capacity. Analyses performed for nanocomposite drug carrier systems; FT-IR, XRD, TGA, BET analysis, Zeta potential, TEM and SEM. Paclitaxel (PTX), a chemotherapeutic drug used in the treatment of pancreatic cancer, was loaded into HAP nanocrystals (PTX- loaded n-HAP) and its activity on pancreatic cancer cells was investigated. When PTX was 1 and 2 mg, Encapsulation Efficiency (EE) and Drug Loading Content (LC) were 79.17-72.24% and 80.01-80.27%, respectively, for H-n-HAP crystal structure only, while EE and LC were 88.57-81.57% and 90.84-110.57%, respectively, when H-n-HAP crystal structure was loaded with 1 and 2 mg PTX together with GO. Here, it was observed PTX release profiles are according to the Hixson model. According to Fick's law, release profile was observed with values of k=1.89, n=0.21, SSD=0.04, R2=0.997, FIC=2.03, SD=0.004. In cell culture studies, as GO nanomaterials were loaded into H-n-HAP nanocrystal structure, the effect of PTX drug on pancreatic cancer increased and the viability of cancer cells decreased. It can be concluded that H-n-HAP/GO/PTX nanocomposite structure kills more pancreatic cancer cells with synergistic effect.
{"title":"Design, Characterization and in vitro Simulations of nano-HAP/GO Composite Drug Delivery System Produced by Hydrothermal Methods Loaded with Paclitaxel","authors":"F. Çiftçi","doi":"10.17776/csj.1258573","DOIUrl":"https://doi.org/10.17776/csj.1258573","url":null,"abstract":"In this study, it was aimed to develop a nano drug system that can be used in passive targeting in pancreatic cancer treatment. Hydroxyapatite nanocrystals (n-HAP) produced by hydrothermal process and graphene oxide (GO) produced by hummers method were used to increase the carrier capacity of the nano drug system and to activate the drug release kinetics and drug loading capacity. Analyses performed for nanocomposite drug carrier systems; FT-IR, XRD, TGA, BET analysis, Zeta potential, TEM and SEM. Paclitaxel (PTX), a chemotherapeutic drug used in the treatment of pancreatic cancer, was loaded into HAP nanocrystals (PTX- loaded n-HAP) and its activity on pancreatic cancer cells was investigated. When PTX was 1 and 2 mg, Encapsulation Efficiency (EE) and Drug Loading Content (LC) were 79.17-72.24% and 80.01-80.27%, respectively, for H-n-HAP crystal structure only, while EE and LC were 88.57-81.57% and 90.84-110.57%, respectively, when H-n-HAP crystal structure was loaded with 1 and 2 mg PTX together with GO. Here, it was observed PTX release profiles are according to the Hixson model. According to Fick's law, release profile was observed with values of k=1.89, n=0.21, SSD=0.04, R2=0.997, FIC=2.03, SD=0.004. In cell culture studies, as GO nanomaterials were loaded into H-n-HAP nanocrystal structure, the effect of PTX drug on pancreatic cancer increased and the viability of cancer cells decreased. It can be concluded that H-n-HAP/GO/PTX nanocomposite structure kills more pancreatic cancer cells with synergistic effect.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43096258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we first investigate the intersection of two different ruled surfaces in R^3 for two different tangential spherical indicatrix curves on DS^2 using the E. Study mapping. The conditions for the intersection of these ruled surfaces in R^3 are expressed by theorems with bivariate functions. Secondly, considering two different principal normal spherical indicatrix curves on DS^2, we examine the intersection of two different ruled surfaces in R^3 by using E. Study mapping. Similarly, the conditions for the intersection of these ruled surfaces in R^3 are indicated by theorems with bivariate functions. Thirdly, using E. Study mapping, we explore the intersection of two different ruled surfaces in R^3 by considering two different binormal spherical indicatrix curves on DS^2. Likewise, the conditions for the intersection of these ruled surfaces in R^3 are denoted by theorems with bivariate functions. Fourthly, considering two different pole spherical indicatrix curves on DS^2, we study the intersection of two different ruled surfaces in R^3 by using E. Study mapping. In the same way, the conditions for the intersection of these ruled surfaces in R^3 are specified by theorems with bivariate functions. Finally, we provide some examples that support the main results.
{"title":"The Intersection of Two Ruled Surfaces Corresponding to Spherical Indicatrix Curves on the Unit Dual Sphere","authors":"Yunus Öztemi̇r, M. Çalişkan","doi":"10.17776/csj.1197746","DOIUrl":"https://doi.org/10.17776/csj.1197746","url":null,"abstract":"In this study, we first investigate the intersection of two different ruled surfaces in R^3 for two different tangential spherical indicatrix curves on DS^2 using the E. Study mapping. The conditions for the intersection of these ruled surfaces in R^3 are expressed by theorems with bivariate functions. Secondly, considering two different principal normal spherical indicatrix curves on DS^2, we examine the intersection of two different ruled surfaces in R^3 by using E. Study mapping. Similarly, the conditions for the intersection of these ruled surfaces in R^3 are indicated by theorems with bivariate functions. Thirdly, using E. Study mapping, we explore the intersection of two different ruled surfaces in R^3 by considering two different binormal spherical indicatrix curves on DS^2. Likewise, the conditions for the intersection of these ruled surfaces in R^3 are denoted by theorems with bivariate functions. Fourthly, considering two different pole spherical indicatrix curves on DS^2, we study the intersection of two different ruled surfaces in R^3 by using E. Study mapping. In the same way, the conditions for the intersection of these ruled surfaces in R^3 are specified by theorems with bivariate functions. Finally, we provide some examples that support the main results.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49112016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In five steps, new compounds 5a, 5b of thiadiazole-hydrazone derivatives were synthesized. Various spectral methods, such as 1H NMR, 13C NMR, and elemental analyses, were used to clarify the structures of the compounds. Three cancer cell lines (MCF7, MDA, and HT-29) and one healthy cell line (L929) were tested for the cytotoxicity activity of synthetic compounds, as well as their inhibitory action against carbonic anhydrase I and II isoenzymes (hCA I and hCA II). Among them, the compound 5b exhibited remarkable CA inhibitory activities compared to a standard inhibitor with IC50 values at of 27 µM for hCA I and 33,46 µM for hCA II. The compounds have been found to be ineffective against cancer cell lines. Furthermore, the compounds were found to be non-toxic to the healthy cell line.
{"title":"Synthesis, Anticancer Activity and Carbonic Anhydrase Inhibitory Activity of new Thiadiazole-hydrazone Derivatives","authors":"H. E. Bostancı, U. Acar Çevik","doi":"10.17776/csj.1225497","DOIUrl":"https://doi.org/10.17776/csj.1225497","url":null,"abstract":"In five steps, new compounds 5a, 5b of thiadiazole-hydrazone derivatives were synthesized. Various spectral methods, such as 1H NMR, 13C NMR, and elemental analyses, were used to clarify the structures of the compounds. Three cancer cell lines (MCF7, MDA, and HT-29) and one healthy cell line (L929) were tested for the cytotoxicity activity of synthetic compounds, as well as their inhibitory action against carbonic anhydrase I and II isoenzymes (hCA I and hCA II). Among them, the compound 5b exhibited remarkable CA inhibitory activities compared to a standard inhibitor with IC50 values at of 27 µM for hCA I and 33,46 µM for hCA II. The compounds have been found to be ineffective against cancer cell lines. Furthermore, the compounds were found to be non-toxic to the healthy cell line.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46561287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, colchicine's cytotoxic effects on SNU-1 cells were examined, and a probable mechanism behind its cytotoxicity was revealed. According to the results of the study, colchicine displayed considerable cytotoxicity with an IC50 value of 14.81ng/ml when it was administered to the cells for 24 hours at different doses ranging from 5 to 100ng/ml. Furthermore, according to mechanistic studies, usege of colchicine significantly increased both early and late apoptotic cells in flow cytometry experiments. The late apoptotic cell population percentage in the control group (5.14 ± 1.27%) dramatically increased to 22.83 ± 1.38% in 14.81ng/ml colchicine treated cells. The early apoptotic cell population percentage in the control group (2.00 ± 1.12%) increased to 6.57 ± 2.35% in 14.81ng/ml colchicine treated cells. ELISA method was used to evaluate how colchicine affects the expression of pro- and anti-apoptotic proteins in SNU-1 cells. Colchicine treatment increased pro-apoptotic Bax and cleaved caspase 3 activities, while anti-apoptotic BCL-2 levels decreased. It is concluded that colchicine increases apoptosis in SNU-1 cells, which leads to an overall increase in cell death. Colchicine's promise as an anticancer drug to treat stomach cancer, however, needs additional research to be determined.
{"title":"Investigation of The Antiproliferative Effect of Colchicine on SNU-1 Gastric Cancer Cells","authors":"Fatih Yulak","doi":"10.17776/csj.1256954","DOIUrl":"https://doi.org/10.17776/csj.1256954","url":null,"abstract":"In this study, colchicine's cytotoxic effects on SNU-1 cells were examined, and a probable mechanism behind its cytotoxicity was revealed. According to the results of the study, colchicine displayed considerable cytotoxicity with an IC50 value of 14.81ng/ml when it was administered to the cells for 24 hours at different doses ranging from 5 to 100ng/ml. Furthermore, according to mechanistic studies, usege of colchicine significantly increased both early and late apoptotic cells in flow cytometry experiments. The late apoptotic cell population percentage in the control group (5.14 ± 1.27%) dramatically increased to 22.83 ± 1.38% in 14.81ng/ml colchicine treated cells. The early apoptotic cell population percentage in the control group (2.00 ± 1.12%) increased to 6.57 ± 2.35% in 14.81ng/ml colchicine treated cells. ELISA method was used to evaluate how colchicine affects the expression of pro- and anti-apoptotic proteins in SNU-1 cells. Colchicine treatment increased pro-apoptotic Bax and cleaved caspase 3 activities, while anti-apoptotic BCL-2 levels decreased. It is concluded that colchicine increases apoptosis in SNU-1 cells, which leads to an overall increase in cell death. Colchicine's promise as an anticancer drug to treat stomach cancer, however, needs additional research to be determined.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42199147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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