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Evaluation of the early effects of the first-dose administration of the Sinovac vaccine on the retina, choroid, and optic disc using optical coherence tomography (OCT) and OCT-angiography. 使用光学相干断层扫描(OCT)和 OCT 血管造影术评估第一剂 Sinovac 疫苗对视网膜、脉络膜和视盘的早期影响。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-09 DOI: 10.1080/15569527.2024.2408683
Yelda Yildiz Tasci, Mehmet Icoz, Sule Gokcek Gurturk Icoz, Ozge Saritas, Mucella Arikan Yorgun, Yasin Toklu

Aim: To determine the effects of the first-dose administration of the Sinovac vaccine on the retina, choroid, and optic disc in healthy participants.

Methods: This prospective design study was conducted with 27 healthy healthcare workers who received the first dose of Sinovac vaccine and 25 healthy controls who were not vaccinated. In the vaccinated group, ophthalmological examinations and measurements were performed before vaccination and one week and one month after vaccination. Subfoveal, nasal, and temporal choroidal thicknesses (CTs), retinal nerve fiber layer (RNFL) thickness, and macular thickness (MT) were determined using spectral domain-optical coherence tomography at all visits. Superficial, deep, and peripapillary radial capillary plexus (superficial capillary plexus, deep capillary plexus (DCP), and radial peripapillary capillary, respectively), choriocapillaris vascular density, and foveal avascular zone parameters were measured on optical coherence tomography-angiography (OCT-A).

Results: No significant difference was detected between the two groups in terms of the parameters measured by OCT and OCT-A (p > 0.05 for all). The CT values measured in all quadrants were significantly higher at the first week after vaccination (p < 0.05 for all), and they returned to their pre-vaccination values at the first month post-vaccination measurement (p > 0.05 for all). Concerning the RNFL and MT values, there was no significant difference between the pre-vaccination and post-vaccination first-week measurements (p > 0.05 for all), but a statistically significant increase was detected in the post-vaccination first-month MT and RNFL measurements (p < 0.05 for all). Only the decreases in the foveal DCP and choriocapillaris vascular density values were significant at the first week after vaccination (p < 0.05 for all).

Conclusion: The early changes detected after vaccination in this study suggest the possibility that autoimmune, vascular, and inflammatory diseases may simultaneously emerge in the early post-vaccination period or may be triggered after vaccination, or that the vaccine may unmask these diseases.

目的:确定首剂接种 Sinovac 疫苗对健康参与者视网膜、脉络膜和视盘的影响:这项前瞻性设计研究的对象是接种第一剂 Sinovac 疫苗的 27 名健康医护人员和未接种疫苗的 25 名健康对照者。接种组在接种前、接种后一周和一个月分别进行了眼科检查和测量。在所有检查中都使用光谱域光学相干断层扫描测定了眼底、鼻腔和颞部脉络膜厚度(CT)、视网膜神经纤维层厚度(RNFL)和黄斑厚度(MT)。光学相干断层扫描-血管造影(OCT-A)测量了浅层、深层和桡侧毛细血管丛(分别为浅层毛细血管丛、深层毛细血管丛(DCP)和桡侧毛细血管丛)、绒毛膜血管密度和眼窝无血管区参数:结果:两组的 OCT 和 OCT-A 测量参数无明显差异(均 p > 0.05)。接种疫苗后第一周,所有象限的 CT 值均明显升高(P 均 > 0.05)。至于 RNFL 和 MT 值,接种前和接种后第一周的测量值之间没有明显差异(均 p > 0.05),但在接种后第一个月的 MT 和 RNFL 测量值中发现了统计学意义上的显著增加(p p 结论):本研究在接种疫苗后发现的早期变化表明,自身免疫性、血管性和炎症性疾病可能在接种疫苗后的早期同时出现,或在接种疫苗后诱发,或疫苗可能掩盖这些疾病。
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引用次数: 0
Choroidal structural changes in inactive thyroid eye disease: a cross-sectional analysis of the choroidal vascularity index. 非活动性甲状腺眼病的脉络膜结构变化:脉络膜血管指数的横断面分析。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-14 DOI: 10.1080/15569527.2024.2412138
Serhat Ermiş, Ece Özal, Fatma Savur, Murat Karapapak

Objective: In this study, we aimed to compare choroidal vascular changes using the Choroidal Vascularity Index (CVI) between patients with inactive Thyroid Eye Disease (iTED) and healthy individuals, and to assess the relationship between CVI and choroidal thickness (CT), smoking history, and clinical parameters.

Methods: In this cross-sectional observational study, the eyes of 30 patients aged 18 to 45 with iTED were compared with the randomly selected eyes of 35 age and gender-matched healthy control individuals. Optical coherence tomography (OCT) scans were taken from all participants. The images were binarized using the ImageJ software, and the total choroidal area (TCA) and luminal area (LA) were measured. The ratio of the LA to the TCA was used to calculate the CVI. The relationships between these measurement parameters and clinical activity score (CAS), exophthalmometry, smoking status, and other clinical parameters were examined.

Results: In the iTED group, CT and CVI values were significantly higher compared to the control group (p < 0.001, p = 0.029). No significant effect of smoking on choroidal parameters was detected in the iTED group. Additionally, there was no statistical correlation between choroidal parameters and either exophthalmometry or CAS (p > 0.05). In the multivariate regression analysis, it was determined that CT showed a significant relationship with thyroid stimulating immunoglobulin (TSI) (p = 0.003).

Conclusions: This study found that CT and CVI were increased in the eyes of patients with iTED compared to healthy controls. The history of smoking did not appear to have any effect on CT and CVI in iTED patients.

研究目的本研究旨在使用脉络膜血管指数(CVI)比较非活动性甲状腺眼病(iTED)患者与健康人之间的脉络膜血管变化,并评估CVI与脉络膜厚度(CT)、吸烟史和临床参数之间的关系:在这项横断面观察性研究中,30 名 18 至 45 岁的 iTED 患者的眼睛与随机选取的 35 名年龄和性别匹配的健康对照者的眼睛进行了比较。所有参与者都进行了光学相干断层扫描(OCT)。使用 ImageJ 软件对图像进行二值化处理,并测量脉络膜总面积(TCA)和管腔面积(LA)。LA 与 TCA 之比用于计算 CVI。研究了这些测量参数与临床活动评分(CAS)、眼外伤测量、吸烟状况和其他临床参数之间的关系:在 iTED 组中,CT 和 CVI 值明显高于对照组(P 0.05)。在多变量回归分析中,CT 与促甲状腺免疫球蛋白(TSI)有显著关系(P = 0.003):本研究发现,与健康对照组相比,iTED 患者眼部的 CT 和 CVI 均有所增加。吸烟史似乎对 iTED 患者的 CT 和 CVI 没有任何影响。
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引用次数: 0
Independent risk factor of drug eruption in immune checkpoint inhibitors treated liver cancer patients: high systemic immune-inflammation index. 接受免疫检查点抑制剂治疗的肝癌患者发生药物爆发的独立风险因素:高全身免疫炎症指数。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-08-24 DOI: 10.1080/15569527.2024.2387597
Hong Yiyong, Huang Ying, Li Xiaodie, Zhu Lin, Zheng Yue, Gong Zijian

Purpose: The clinical application of immune checkpoint inhibitors (ICIs) has significantly improved the prognosis of liver cancer patients. However, drug eruption associated with ICI monotherapy or combination therapy not only impacts the quality of life and treatment progress of liver cancer patients but also poses a potential threat to their lives. The study aims to investigate the risk factors of drug eruption in liver cancer patients undergoing ICIs in real-world settings.

Methods: We retrospectively collected data from liver cancer patients who underwent ICI therapies at the Third Affiliated Hospital of Sun Yat-sen University between 2021 and 2022. A propensity score matching (PSM) method was employed to match 31 liver cancer patients with ICI-related drug eruption (drug eruption group) to 228 liver cancer patients without immune-related adverse reactions (control group) in a 1:2 ratio, creating two groups of patients with comparable baseline characteristics. Subsequently, logistic regression analysis was then conducted to analyze the clinical risk factors associated with drug eruption caused by ICIs.

Results: Before PSM, there were statistically significant differences between the drug eruption group (31 cases) and the control group (228 cases) in two variables: Child-Pugh liver function classification and presence of vascular invasion (both p < 0.05). However, after PSM, no statistically significant differences were found in the clinical variables between the drug eruption group (28 cases) and the control group (52 cases). Univariate analysis revealed significantly higher levels of aspartate amino-transferase, alanine aminotransferase, glutamyl transpeptidase, and systemic immune-inflammation index (SII) and a significantly lower rate of liver cancer resection surgery before immunotherapy in liver cancer patients with drug eruption compared to the control group (p < 0.05). Multivariate analysis indicated that an elevated SII level before immunotherapy was significantly associated with the occurrence of drug eruption in liver cancer patients treated with ICIs (p < 0.05). The predictive performance of SII before immunotherapy in liver cancer patients for ICI-related drug eruption yielded an area under the receiver operator characteristic curve of 0.852, with a critical value of 749.189. Sensitivity and specificity were determined as 85.7% and 75%, respectively (p < 0.05).

Conclusions: Elevated systemic immune-inflammation index is identified as a risk factor for drug eruption occurrence in liver cancer patients treated with ICI therapies.

目的:免疫检查点抑制剂(ICIs)的临床应用大大改善了肝癌患者的预后。然而,与 ICI 单药或联合治疗相关的药物喷发不仅影响肝癌患者的生活质量和治疗进展,还对其生命构成潜在威胁。本研究旨在调查在真实世界环境中接受 ICIs 治疗的肝癌患者发生药物过敏的风险因素:我们回顾性地收集了2021年至2022年间在中山大学附属第三医院接受ICI治疗的肝癌患者的数据。采用倾向得分匹配法(PSM),将31例发生ICI相关药物不良反应的肝癌患者(药物不良反应组)与228例未发生免疫相关不良反应的肝癌患者(对照组)按1:2的比例进行匹配,形成两组基线特征具有可比性的患者。然后,进行逻辑回归分析,分析与 ICIs 引起药物过敏相关的临床风险因素:在 PSM 之前,药物糜烂组(31 例)与对照组(228 例)在两个变量上存在显著统计学差异:Child-Pugh肝功能分级和是否存在血管侵犯(均为p p p p 结论:药物糜烂组(31例)与对照组(228例)在 PSM前在两个变量上存在统计学差异:全身免疫炎症指数升高是接受 ICI 治疗的肝癌患者发生药物性肝癌的危险因素。
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引用次数: 0
The effects of electronic cigarette smoking on retinal nerve fiber layer, ganglion cell layer, inner plexiform layer, and choroidal thickness. 吸电子烟对视网膜神经纤维层、神经节细胞层、内丛状层和脉络膜厚度的影响。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-05 DOI: 10.1080/15569527.2024.2423265
Dharshini Balasubaramaniam, Yi Wen Lim, Shamala Retnasabapathy, Fazilawati A Qamarruddin, Sujaya Singh

Purpose: To compare the thickness of the retinal nerve fibre layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), and choroid in healthy electronic cigarette smokers and non-smokers using spectral domain optical coherence tomography (SD-OCT).

Material and method: 25 healthy electronic cigarette smokers and 25 age- and gender-matched healthy non-smokers were included in the study. RNFL, GCL, IPL and choroidal thickness were measured by SD-OCT using an automated programme. After normality tests, an independent sample t-test was used to analyse the differences in RNFL, GCL, IPL, and choroidal thickness values between the groups.

Results: The mean age of electronic cigarette smokers and non-smokers was 33.68 and 33.64 years, respectively. The mean smoking history was 6.6 years (range 5-8 years). Most of the participants smoked 2-5 ml of e-liquid per day (52%), while 36% smoked more than 5 ml and 12% smoked less than 2 ml per day. The mean intraocular pressure in the electronic cigarette smoker group was 15.0 mmHg, while the non-smoker group was 15.32 mmHg. The mean axial length in the electronic cigarette smoker group and non-smoker group was 23.36 and 23.63 mm, respectively. No significant difference was observed regarding RNFL, GCL, IPL or choroidal thickness between both groups.

Conclusion: The thickness of the RNFL, GCL, IPL, and choroid was found to be similar in both the healthy electronic cigarette smokers and non-smokers groups.

目的:使用光谱域光学相干断层扫描(SD-OCT)比较健康电子烟吸烟者和非吸烟者的视网膜神经纤维层(RNFL)、神经节细胞层(GCL)、内丛状层(IPL)和脉络膜的厚度。使用自动程序通过 SD-OCT 测量 RNFL、GCL、IPL 和脉络膜厚度。经过正态性检验后,采用独立样本 t 检验分析各组间 RNFL、GCL、IPL 和脉络膜厚度值的差异:电子烟吸烟者和非吸烟者的平均年龄分别为 33.68 岁和 33.64 岁。平均吸烟史为 6.6 年(5-8 年不等)。大多数参与者(52%)每天吸 2-5 毫升电子烟液,36% 吸 5 毫升以上,12% 吸 2 毫升以下。电子烟吸食者组的平均眼压为 15.0 毫米汞柱,而非吸食者组为 15.32 毫米汞柱。电子烟吸烟者组和非吸烟者组的平均眼轴长度分别为 23.36 毫米和 23.63 毫米。两组的 RNFL、GCL、IPL 或脉络膜厚度均无明显差异:结论:健康的电子烟吸烟者组和非吸烟者组的 RNFL、GCL、IPL 和脉络膜厚度相似。
{"title":"The effects of electronic cigarette smoking on retinal nerve fiber layer, ganglion cell layer, inner plexiform layer, and choroidal thickness.","authors":"Dharshini Balasubaramaniam, Yi Wen Lim, Shamala Retnasabapathy, Fazilawati A Qamarruddin, Sujaya Singh","doi":"10.1080/15569527.2024.2423265","DOIUrl":"10.1080/15569527.2024.2423265","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the thickness of the retinal nerve fibre layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), and choroid in healthy electronic cigarette smokers and non-smokers using spectral domain optical coherence tomography (SD-OCT).</p><p><strong>Material and method: </strong>25 healthy electronic cigarette smokers and 25 age- and gender-matched healthy non-smokers were included in the study. RNFL, GCL, IPL and choroidal thickness were measured by SD-OCT using an automated programme. After normality tests, an independent sample <i>t</i>-test was used to analyse the differences in RNFL, GCL, IPL, and choroidal thickness values between the groups.</p><p><strong>Results: </strong>The mean age of electronic cigarette smokers and non-smokers was 33.68 and 33.64 years, respectively. The mean smoking history was 6.6 years (range 5-8 years). Most of the participants smoked 2-5 ml of e-liquid per day (52%), while 36% smoked more than 5 ml and 12% smoked less than 2 ml per day. The mean intraocular pressure in the electronic cigarette smoker group was 15.0 mmHg, while the non-smoker group was 15.32 mmHg. The mean axial length in the electronic cigarette smoker group and non-smoker group was 23.36 and 23.63 mm, respectively. No significant difference was observed regarding RNFL, GCL, IPL or choroidal thickness between both groups.</p><p><strong>Conclusion: </strong>The thickness of the RNFL, GCL, IPL, and choroid was found to be similar in both the healthy electronic cigarette smokers and non-smokers groups.</p>","PeriodicalId":11023,"journal":{"name":"Cutaneous and Ocular Toxicology","volume":" ","pages":"390-395"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective cytotoxicity of standardised n-hexane extract of black soldier flies' larvae on cancerous skin cells mitochondria isolated from rat model of melanoma. 标准化正己烷提取物对从黑色素瘤大鼠模型中分离出的皮肤癌细胞线粒体的选择性细胞毒性。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-08-14 DOI: 10.1080/15569527.2024.2389193
Yalda Arast, Mahya Sabbaghi, Farzane Kamranfar, Fatemeh Heidari, Seyed Mojtaba Fazli Nejad, Tahereh Hosseinabadi, Jalal Pourahmad

Introduction: Melanoma is known as a highly lethal cancer. In melanoma cells, apoptosis signalling which relies heavily on the acute activity of mitochondria and reactive oxygen species (ROS) formation is suppressed. Our previous studies on natural compounds on melanoma suggested that mitochondria are a potential target for the melanoma treatment by selective cytotoxic effect of them. The black soldier fly is an important environmental protectant insect that based on recent studies induces apoptosis in liver and colorectal carcinoma cells through the activation of caspase 3, 8, and 9 and ultimately inhibits the growth of cancer cells.

Purpose: This study was designed to evaluate the selective apoptotic effect of the n-hexane BSFL extract (BSFLE) on skin mitochondria.

Materials and methods: The mitochondria isolated from melanoma cells were treated with various concentrations (1500, 3000, and 6000 µg/ml) of n-hexane BSFLE Then MTT viability assay, ROS determination, Mitochondrial Membrane Potential (MMP), mitochondrial swelling, cytochrome c release determination, and % apoptosis were performed.

Results: MTT assay showed that different concentrations of n-hexane BSFLE significantly (P < 0.05) decreased the SDH activity in cancerous skin mitochondria with the IC50. The ROS production and mitochondrial swelling results also showed that all concentrations of BSFL extracts significantly increased. MMP decline and the release of cytochrome c in cancer groups mitochondria. BSFLE increased apoptosis on melanoma cells.

Discussion and conclusion: It is suggested that n-hexane BSFLE compounds selectively induce a cascade of proapoptotic events that are probably defective in cancer cells. Most of these compounds target the mitochondrial transient pore caused by disruption of the mitochondrial respiratory chain. These events lead to disruption of the temporary permeability of mitochondria, swelling of mitochondria and finally the formation of apoptosome in the cytosol.

简介众所周知,黑色素瘤是一种致死率极高的癌症。黑色素瘤细胞的凋亡信号主要依赖线粒体的急性活动和活性氧(ROS)的形成。我们之前关于天然化合物对黑色素瘤的研究表明,线粒体是治疗黑色素瘤的潜在靶点,它们具有选择性细胞毒性作用。目的:本研究旨在评估正己烷 BSFL 提取物(BSFLE)对皮肤线粒体的选择性凋亡作用。材料与方法:用不同浓度(1500、3000 和 6000 µg/ml)的正己烷 BSFLE 处理从黑色素瘤细胞中分离出的线粒体,然后进行 MTT 活力测定、ROS 测定、线粒体膜电位(MMP)、线粒体肿胀、细胞色素 c 释放测定和细胞凋亡率测定:MTT 检测显示,不同浓度的正己烷 BSFLE 对癌细胞的凋亡有显著影响(P 讨论和结论:这表明正己烷 BSFLE 化合物可选择性地诱导一连串的促凋亡事件,而这些事件在癌细胞中可能是有缺陷的。这些化合物大多以线粒体瞬时孔为目标,而线粒体瞬时孔是由线粒体呼吸链的破坏引起的。这些事件导致线粒体的暂时通透性被破坏,线粒体膨胀,最后在细胞质中形成凋亡小体。
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引用次数: 0
Correction. 更正。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-19 DOI: 10.1080/15569527.2024.2428898
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引用次数: 0
Effect of adalimumab treatment on inflammatory and hematological parameters in patients with Hidradenitis suppurativa. 阿达木单抗治疗对化脓性扁平湿疹患者炎症和血液指标的影响
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-08-11 DOI: 10.1080/15569527.2024.2387595
Kadir Kaya, Özlem Gevher

Objective: Hidradenitis suppurativa (HS), a chronic inflammatory disease that typically manifests after puberty, is characterised by painful nodules, abscesses, draining sinus tracts, and scars in areas rich in apocrine glands such as the axillary and inguinal regions. In recent years, blood-based biomarkers such as the Neutrophil/Lymphocyte Ratio (NLR), Platelet/Lymphocyte Ratio (PLR), Monocyte/Lymphocyte Ratio (MLR), Mean Platelet Volume (MPV), Systemic Immune-Inflammation Index (SII) and Pan-Immune-Inflammation Value (PIV) have been used as significant indicators of systemic inflammation. While there are few studies evaluating these biomarkers in HS, the response of these markers to treatment has only been assessed in one study to date. Our study aims to investigate the effect of adalimumab treatment on blood-based systemic inflammation biomarkers in HS, where inflammation plays a significant role.

Methods: The study included 42 adult patients who received adalimumab treatment at our dermatology and venereology clinic between January 2020 and January 2023. Medical records for complete blood count results of the patients were retrospectively reviewed. All systemic inflammation-based biomarkers were calculated from the absolute values of the complete blood count. The SII was calculated with the following formula: (neutrophil count × platelet count/lymphocyte count). The PIV was calculated as follows: (neutrophil count × platelet count × monocyte count/lymphocyte count). Values before the treatment and at the 12th week of treatment were compared.

Results: When the changes in the inflammatory parameters of the patients were examined, it was found that NLR (2.13 ± 0.87 vs 2.26 ± 1.12), PLR (111.01 ± 39.89 vs 99.43 ± 35.34), MLR (0.27 ± 0.11 vs 0.28 ± 0.12), MPV (9.59 ± 0.71 vs 9.70 ± 0.79), SII (680.79 ± 330.18 vs 687.89 ± 442.66), and PIV (552.02 ± 330.71 vs 605.05 ± 415.96) values did not change statistically significantly after treatment (p > 0.05). While there was a significant decrease in platelet count compared to before treatment, no statistically significant difference was found in the other evaluated blood cells.

Conclusion: Adalimumab treatment has not had a significant effect on systemic inflammation markers in HS, an inflammatory disease. More studies are needed to evaluate the effect of adalimumab on these markers in HS.

目的:化脓性扁平湿疹(HS)是一种慢性炎症性疾病,通常在青春期后发病,其特征是在腋窝和腹股沟等分泌腺丰富的部位出现疼痛性结节、脓肿、引流窦道和疤痕。近年来,中性粒细胞/淋巴细胞比值(NLR)、血小板/淋巴细胞比值(PLR)、单核细胞/淋巴细胞比值(MLR)、平均血小板体积(MPV)、全身免疫炎症指数(SII)和泛免疫炎症值(PIV)等基于血液的生物标志物已被用作全身炎症的重要指标。虽然评估 HS 中这些生物标志物的研究不多,但迄今为止只有一项研究评估了这些标志物对治疗的反应。我们的研究旨在探讨阿达木单抗治疗对HS血液系统炎症生物标志物的影响,因为炎症在HS中起着重要作用:研究纳入了2020年1月至2023年1月期间在我院皮肤病与性病诊所接受阿达木单抗治疗的42名成年患者。对患者的全血细胞计数结果的医疗记录进行了回顾性审查。所有以全身炎症为基础的生物标志物都是根据全血细胞计数的绝对值计算得出的。SII 用以下公式计算:(中性粒细胞计数 x 血小板计数/淋巴细胞计数)。PIV 的计算公式如下(中性粒细胞计数 x 血小板计数 x 单核细胞计数/淋巴细胞计数)。比较治疗前和治疗第 12 周时的数值:检查患者炎症参数的变化时发现,NLR(2,13 ± 0,87 vs 2,26 ± 1,12)、PLR(111,01 ± 39,89 vs 99,43 ± 35,34)、MLR(0,27 ± 0,11 vs 0,28 ± 0、12)、MPV(9,59 ± 0,71 vs 9,70 ± 0,79)、SII(680,79 ± 330,18 vs 687,89 ± 442,66)和 PIV(552,02 ± 330,71 vs 605,05 ± 415,96)值在治疗后没有显著的统计学变化(P > 0.05).与治疗前相比,血小板数量明显减少,但其他被评估的血细胞在统计学上没有明显差异:结论:阿达木单抗治疗对HS这种炎症性疾病的全身炎症指标没有明显影响。结论:阿达木单抗治疗对HS这种炎症性疾病的全身炎症指标没有明显影响,还需要更多研究来评估阿达木单抗对HS这些指标的影响。
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引用次数: 0
Correction. 修正。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-12-02 DOI: 10.1080/15569527.2024.2428043
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引用次数: 0
Intravitreal chlorhexidine for sterilizing the vitreous cavity in an animal model of bacterial endophthalmitis. 玻璃体内氯己定对细菌性眼内炎动物模型玻璃体腔的消毒作用。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-09-18 DOI: 10.1080/15569527.2024.2402410
Ahmet M Hondur, Qun Zeng, Yucel Ucgul, Idil Arioz, Elif Ayca Sahin, Gamze Gizem Erayman, Ayse B Tekinay, Kayhan Caglar, Nalan Akyurek, Tongalp H Tezel

Purpose: To evaluate the efficacy and safety of intravitreal chlorhexidine (CHX) for sterilising the vitreous cavity in bacterial endophthalmitis.

Methods: For in-vitro experiments, full-thickness retina explants were harvested from freshly enucleated pig eyes. Six-millimeter circular sensory retina patches were then incubated in varying concentrations of CHX (0.625-800 µg/mL) for 24 hours. Retinal cell viability was determined at the end of the incubation period with a live-dead assay. The bactericidal effects of the tested CHX concentrations were determined using a quantitative suspension test on Staphylococcus epidermidis. The safety of CHX was also tested by injecting varying doses of CHX (50-400 µg/mL) into the vitreous cavity of albino rabbits followed by flash electroretinography (ERG) and light microscopy. The bactericidal effect of the non-toxic CHX doses was determined using the rabbit model of endophthalmitis created by injecting 3000 CFU/0.1 mL of Staphylococcus epidermidis.

Results: In vitro concentrations of CHX greater than 6.25 µgr/mL exerted a bactericidal effect, while concentrations of CHX less than 200 µg/mL did not impair retinal cell viability. Intravitreal concentrations of CHX between 20-100 µg/mL were adequate to sterilise the infected rabbit vitreous cavity in the animal model. No significant functional or anatomical deleterious effect was observed with ERG or light microscopy.

Conclusion: CHX can sterilise the vitreous cavity in an animal model of bacterial endophthalmitis without impairing retinal cell viability. Our results encourage further research for clinical use of chlorhexidine in treatment of bacterial endophthalmitis.

目的:评价玻璃体内注射氯己定(CHX)对细菌性眼内炎玻璃体腔消毒的疗效和安全性。方法:采用新鲜去核猪眼全层视网膜外植体进行体外实验。6毫米圆形感觉视网膜斑块在不同浓度的CHX(0.625-800µg/mL)中孵育24小时。视网膜细胞活力在孵育期结束时用活死法测定。采用定量悬液法测定CHX浓度对表皮葡萄球菌的杀菌效果。在白化兔玻璃体腔内注射不同剂量的CHX(50-400µg/mL),并采用闪烁视网膜电图(ERG)和光镜观察CHX的安全性。采用表皮葡萄球菌(Staphylococcus epidermidis) 3000 CFU/0.1 mL致兔眼内炎模型,观察无毒剂量CHX的杀菌效果。结果:体外浓度大于6.25µg/mL的CHX具有杀菌作用,而浓度小于200µg/mL的CHX不影响视网膜细胞活力。玻璃体内CHX浓度在20-100µg/mL之间足以对动物模型中感染的兔玻璃体腔进行消毒。在ERG或光镜下未观察到明显的功能或解剖上的有害影响。结论:CHX能对细菌性眼内炎动物模型玻璃体腔进行消毒,但不影响视网膜细胞活力。我们的结果鼓励进一步研究氯己定治疗细菌性眼内炎的临床应用。
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引用次数: 0
Research on the damage and wound repair of cornea by GO and ZnO. 研究 GO 和氧化锌对角膜的损伤和伤口修复。
IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-12-01 Epub Date: 2024-08-12 DOI: 10.1080/15569527.2024.2387592
Hanzhu Zhao, Aiping Song, Liyan Wang, Xiaolu Hou, Dongmei Cui, Xiaotong Sun, Lingzhi Niu, Lin Jin, Haoyuan An, Wei Li

Objective: The widespread use of nanoparticles in recent years has increased the risk of ocular exposure. zinc oxide (ZnO) is widely used in the field of cosmetics because of its unique chemical properties. The application of graphene oxide (GO) as an emerging nanomaterial in the field of eye drops is also gradually emerging. Currently, research on ZnO and GO eye exposure mainly focuses on application or toxicity to optic nerve cells. There's less study on corneal wound healing effects. and the previous research hasn't compared ZnO and GO corneal toxicity.

Methods: We systematically established a complete chain study of in vitro and in vivo experiments and mouse corneal injury model, and comprehensively evaluated the ocular safety and toxicity of ZnO and GO.

Results: We found that 50 ug/mL GO and 0.5 ug/mL ZnO can reduce human corneal epithelial cells (HCEpiC) viability in a concentration-dependent manner. Short-term repeated exposure to ZnO can cause sterile inflammation of the cornea with concentration-dependence, while GO have not been significantly altered. 50 ug/mL ZnO could significantly delay the healing of corneal wounds, while GO did not change wound healing.

Conclusion: The toxic effect of ZnO is higher than that of GO. Inflammatory signal transduction, oxidative stress and apopnano zitosis are involved in the ocular toxicity injury process of nanoparticles. Research can provide a judgement basis for people's eye health and eye protection risk control.

目的:氧化锌(ZnO)因其独特的化学特性被广泛应用于化妆品领域。氧化石墨烯(GO)作为一种新兴的纳米材料,在滴眼液领域的应用也逐渐兴起。目前,有关氧化锌和 GO 暴露于眼睛的研究主要集中在对视神经细胞的应用或毒性方面。方法:我们系统地建立了一个完整的研究链条,研究了氧化锌和氧化亚铜对角膜伤口愈合的影响:方法:我们系统地建立了一个完整的体内外实验链研究和小鼠角膜损伤模型,全面评价了氧化锌和 GO 的眼部安全性和毒性:结果:我们发现,50微克/毫升的GO和0.5微克/毫升的氧化锌能以浓度依赖的方式降低人角膜上皮细胞(HCEpiC)的活力。短期反复接触氧化锌可导致角膜无菌性炎症,且与浓度有关,而 GO 则没有明显改变。50 微克/毫升的氧化锌可明显延迟角膜伤口的愈合,而 GO 则不会改变伤口的愈合:结论:ZnO 的毒性效应高于 GO。结论:ZnO 的毒性作用高于 GO,炎症信号转导、氧化应激和凋亡参与了纳米颗粒的眼毒性损伤过程。相关研究可为人们的眼健康和眼保护风险控制提供判断依据。
{"title":"Research on the damage and wound repair of cornea by GO and ZnO.","authors":"Hanzhu Zhao, Aiping Song, Liyan Wang, Xiaolu Hou, Dongmei Cui, Xiaotong Sun, Lingzhi Niu, Lin Jin, Haoyuan An, Wei Li","doi":"10.1080/15569527.2024.2387592","DOIUrl":"10.1080/15569527.2024.2387592","url":null,"abstract":"<p><strong>Objective: </strong>The widespread use of nanoparticles in recent years has increased the risk of ocular exposure. zinc oxide (ZnO) is widely used in the field of cosmetics because of its unique chemical properties. The application of graphene oxide (GO) as an emerging nanomaterial in the field of eye drops is also gradually emerging. Currently, research on ZnO and GO eye exposure mainly focuses on application or toxicity to optic nerve cells. There's less study on corneal wound healing effects. and the previous research hasn't compared ZnO and GO corneal toxicity.</p><p><strong>Methods: </strong>We systematically established a complete chain study of in vitro and in vivo experiments and mouse corneal injury model, and comprehensively evaluated the ocular safety and toxicity of ZnO and GO.</p><p><strong>Results: </strong>We found that 50 ug/mL GO and 0.5 ug/mL ZnO can reduce human corneal epithelial cells (HCEpiC) viability in a concentration-dependent manner. Short-term repeated exposure to ZnO can cause sterile inflammation of the cornea with concentration-dependence, while GO have not been significantly altered. 50 ug/mL ZnO could significantly delay the healing of corneal wounds, while GO did not change wound healing.</p><p><strong>Conclusion: </strong>The toxic effect of ZnO is higher than that of GO. Inflammatory signal transduction, oxidative stress and apopnano zitosis are involved in the ocular toxicity injury process of nanoparticles. Research can provide a judgement basis for people's eye health and eye protection risk control.</p>","PeriodicalId":11023,"journal":{"name":"Cutaneous and Ocular Toxicology","volume":" ","pages":"237-252"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Cutaneous and Ocular Toxicology
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