Agustin Wulan Suci Dharmayanti, Erfin Ramadana Pratama, B. Kusumawardani, Z. Hamzah, S. Suhartini, T. Indriana, Dwi Warna Aju Fatmawati
Stress is a condition that burdens a person and affects homeostasis resulting in health problems, including dental and oral health. Stress can be caused by physical, chemical, or psychological stressors. Stressors cause an increase in blood glucose levels due to an increase in the stress hormone cortisol. This study aims to determine the effect of physical-psychological and psychological stress induced by electrical foot shock on blood glucose levels in rats. This research is an experimental laboratory study with a post-test control group design using white male rats as animal stress models. Animal models were divided into six groups, namely physical-psychological stress for 7 days (PPS1), 14 days (PPS2), and 28 days (PPS3); and psychological stress for 7 days (PS1), 14 days (PS2), and 28 days (PS3). The animal models were placed in the experimental box with electrical foot shock. The physical-psychological stress treatment was to flow an electric current of 48 V, 0.5 Hz, 2 mA for 30 minutes daily. In the psychological stress treatment, the animal models were placed without an electric shock, but the animal models were placed together, given an electric shock stressor separated by transparent plastic with a thickness of 5 cm and foam at the bottom of the cage (insulator). The results showed that although there was no effect of electrical foot shock on blood glucose levels in rats experiencing physical-psychological stress for 7, 14, and 28 exposures (p0.05), electrical foot shock affected blood glucose levels in rats with psychological stress (p0.05). It can be concluded that physical-psychological and psychological stress induced by electrical foot shock affected blood glucose levels.
压力是一种给人带来负担并影响体内平衡的状况，导致健康问题，包括牙齿和口腔健康。压力可以由物理、化学或心理压力源引起。由于压力荷尔蒙皮质醇的增加，压力源会导致血糖水平升高。本研究旨在探讨足电诱发的生理-心理和心理应激对大鼠血糖水平的影响。本研究是一项实验实验室研究，采用测试后对照组设计，以白种雄性大鼠为动物应激模型。动物模型分为6组，即生理-心理应激7 d (PPS1)、14 d (PPS2)和28 d (PPS3);心理应激7 d (PS1)、14 d (PS2)、28 d (PS3)。将动物模型置于足部电击实验箱中。生理-心理应激处理为48 V, 0.5 Hz, 2 mA电流，每天30分钟。在心理应激治疗中，动物模型不电击放置，但将动物模型放在一起，在笼底用厚度为5 cm的透明塑料和泡沫(绝缘体)隔开电击应激源。结果显示，虽然足电对生理-心理应激大鼠的血糖水平没有影响(p0.05)，但足电对心理应激大鼠的血糖水平有影响(p0.05)。由此可见，足电诱发的生理-心理和心理应激对血糖水平有影响。
{"title":"The Effect of Physical-Psychological and Psychological Stress on Blood Glucose Levels (In vivo Study)","authors":"Agustin Wulan Suci Dharmayanti, Erfin Ramadana Pratama, B. Kusumawardani, Z. Hamzah, S. Suhartini, T. Indriana, Dwi Warna Aju Fatmawati","doi":"10.18196/di.v12i1.13945","DOIUrl":"https://doi.org/10.18196/di.v12i1.13945","url":null,"abstract":"Stress is a condition that burdens a person and affects homeostasis resulting in health problems, including dental and oral health. Stress can be caused by physical, chemical, or psychological stressors. Stressors cause an increase in blood glucose levels due to an increase in the stress hormone cortisol. This study aims to determine the effect of physical-psychological and psychological stress induced by electrical foot shock on blood glucose levels in rats. This research is an experimental laboratory study with a post-test control group design using white male rats as animal stress models. Animal models were divided into six groups, namely physical-psychological stress for 7 days (PPS1), 14 days (PPS2), and 28 days (PPS3); and psychological stress for 7 days (PS1), 14 days (PS2), and 28 days (PS3). The animal models were placed in the experimental box with electrical foot shock. The physical-psychological stress treatment was to flow an electric current of 48 V, 0.5 Hz, 2 mA for 30 minutes daily. In the psychological stress treatment, the animal models were placed without an electric shock, but the animal models were placed together, given an electric shock stressor separated by transparent plastic with a thickness of 5 cm and foam at the bottom of the cage (insulator). The results showed that although there was no effect of electrical foot shock on blood glucose levels in rats experiencing physical-psychological stress for 7, 14, and 28 exposures (p0.05), electrical foot shock affected blood glucose levels in rats with psychological stress (p0.05). It can be concluded that physical-psychological and psychological stress induced by electrical foot shock affected blood glucose levels.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86030156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Rizal, Widyasri Prananingrum, Dian W. Damaiyanti
The prevalence of dental caries in Indonesia is still high. One of the dental caries treatments is direct restoration. Biomaterial, often used as a restorative material, is glass ionomer cement which has a silica content of 35%. Silica is found in Penaeus monodon shells which have been a waste of production. This research aims to study the characteristic of Penaeus monodon shell powder extracts as candidates for dental materials restoration. The research sample used was Penaeus monodon shell powder extract which was then carried out by a depigmentation process by mixing acetone 1:10 (w/v) ≤ 20 hours. Samples were tested with the XRD test (x-ray diffraction). SEM assessment was performed to evaluate the morphology of the particle. Data were analyzed using Mann-Whitney. The result demonstrated a significant silica content in Penaeus monodon shell powder extract before and after the depigmentation process with a p-value of 0,00 (p0,05). The depigmentation process can decrease silica content in Penaeus monodon shell powder extract. There was a different comparison of silica content in Penaeus monodon shell powder extract before and after depigmentation as a candidate for dental materials restoration.
{"title":"Penaeus monodon Shell Powder Extract as A Candidate for Dental Material Restoration","authors":"M. Rizal, Widyasri Prananingrum, Dian W. Damaiyanti","doi":"10.18196/di.v12i1.16299","DOIUrl":"https://doi.org/10.18196/di.v12i1.16299","url":null,"abstract":"The prevalence of dental caries in Indonesia is still high. One of the dental caries treatments is direct restoration. Biomaterial, often used as a restorative material, is glass ionomer cement which has a silica content of 35%. Silica is found in Penaeus monodon shells which have been a waste of production. This research aims to study the characteristic of Penaeus monodon shell powder extracts as candidates for dental materials restoration. The research sample used was Penaeus monodon shell powder extract which was then carried out by a depigmentation process by mixing acetone 1:10 (w/v) ≤ 20 hours. Samples were tested with the XRD test (x-ray diffraction). SEM assessment was performed to evaluate the morphology of the particle. Data were analyzed using Mann-Whitney. The result demonstrated a significant silica content in Penaeus monodon shell powder extract before and after the depigmentation process with a p-value of 0,00 (p0,05). The depigmentation process can decrease silica content in Penaeus monodon shell powder extract. There was a different comparison of silica content in Penaeus monodon shell powder extract before and after depigmentation as a candidate for dental materials restoration.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86973689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suryono Suryono, Muhammad Reza Pahlevi, Nisaul Afifah, Prayitno Prayitno
Gingivitis adalah peradangan gingiva yang disebabkan oleh akumulasi plak subgingiva. Plak akan meningkatkan aktivitas sel fagosit dan mediator inflamasi. Infiltrasi sel inflamasi terutama neutrofil polimorfonuklear akan menyebabkan inflamasi yang dapat diamati secara klinis. Indeks gingiva menunjukkan derajat inflamasi pada gingivitis. Penurunan indeks gingiva dapat disimpulkan sebagai penyembuhan peradangan. Pengobatan gingivitis adalah scaling, root planning, dan terapi obat anti inflamasi non steroid (NSAID). Terapi menggunakan NSAID merupakan upaya untuk memodulasi respon host pada inflamasi. Biji pepaya dapat digunakan untuk memodulasi respon inang karena mengandung flavonoid, saponin, dan polifenol yang memiliki efek antiinflamasi, antibakteri dan antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak biji pepaya terhadap derajat inflamasi pada gingivitis tikus wistar. Tiga puluh ekor tikus wistar dibagi menjadi 5 kelompok yaitu kontrol negatif, kontrol positif, dan tiga kelompok ekstrak biji pepaya dengan konsentrasi 0,2%, 2% dan 20%. Gingivitis diinduksi dengan menempatkan ligatur sutera retentif plak pada gigi seri mandibula dan Aggregatibacter actinomycetemcomitans inokulasi sulkus gingiva. Ekstrak diterapkan dua kali sehari. Tikus didekapitasi pada hari kedua dan kelima kemudian dibuat preparat histologi fragmen gingiva. Perbedaan yang signifikan diamati pada indeks gingiva antara kelompok perlakuan dan kontrol negatif. Penelitian ini juga menemukan penurunan indeks gingiva setelah perawatan dengan ekstrak biji pepaya. Kemudian disimpulkan bahwa ekstrak biji pepaya dapat menghambat inflamasi dan menurunkan derajat inflamasi pada gingivitis tikus wistar.
{"title":"Pengaruh ekstrak biji pepaya (Carica papaya Linn) terhadap tingkat inflamasi gingivitis (studi in vivo pada Rattus norvegicus)","authors":"Suryono Suryono, Muhammad Reza Pahlevi, Nisaul Afifah, Prayitno Prayitno","doi":"10.22146/mkgk.83698","DOIUrl":"https://doi.org/10.22146/mkgk.83698","url":null,"abstract":"Gingivitis adalah peradangan gingiva yang disebabkan oleh akumulasi plak subgingiva. Plak akan meningkatkan aktivitas sel fagosit dan mediator inflamasi. Infiltrasi sel inflamasi terutama neutrofil polimorfonuklear akan menyebabkan inflamasi yang dapat diamati secara klinis. Indeks gingiva menunjukkan derajat inflamasi pada gingivitis. Penurunan indeks gingiva dapat disimpulkan sebagai penyembuhan peradangan. Pengobatan gingivitis adalah scaling, root planning, dan terapi obat anti inflamasi non steroid (NSAID). Terapi menggunakan NSAID merupakan upaya untuk memodulasi respon host pada inflamasi. Biji pepaya dapat digunakan untuk memodulasi respon inang karena mengandung flavonoid, saponin, dan polifenol yang memiliki efek antiinflamasi, antibakteri dan antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak biji pepaya terhadap derajat inflamasi pada gingivitis tikus wistar. Tiga puluh ekor tikus wistar dibagi menjadi 5 kelompok yaitu kontrol negatif, kontrol positif, dan tiga kelompok ekstrak biji pepaya dengan konsentrasi 0,2%, 2% dan 20%. Gingivitis diinduksi dengan menempatkan ligatur sutera retentif plak pada gigi seri mandibula dan Aggregatibacter actinomycetemcomitans inokulasi sulkus gingiva. Ekstrak diterapkan dua kali sehari. Tikus didekapitasi pada hari kedua dan kelima kemudian dibuat preparat histologi fragmen gingiva. Perbedaan yang signifikan diamati pada indeks gingiva antara kelompok perlakuan dan kontrol negatif. Penelitian ini juga menemukan penurunan indeks gingiva setelah perawatan dengan ekstrak biji pepaya. Kemudian disimpulkan bahwa ekstrak biji pepaya dapat menghambat inflamasi dan menurunkan derajat inflamasi pada gingivitis tikus wistar.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135648684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.20473/j.djmkg.v56.i2.p122-126
Hilda Fitria Lubis, Hanifa Natarisya
Background: Beta titanium orthodontic wire is known to have good corrosion resistance but is weak in acidic environments, which advance the corrosion rate. One natural inhibitor that can be used to decrease corrosion is kiwi peel extract, which has a high antioxidant level. Purpose: This study aims to examine the ability of the extract to decrease the corrosion rate and microstructural changes of beta titanium at an acidic pH (pH 5). Methods: The samples used were beta titanium with a diameter of 0.016 x 0.022 in and a length of 6 cm. A total of 28 samples (n=28) were divided into four groups—a control group immersed in pH 5 artificial saliva and three treatment groups immersed in kiwi peel extract at concentrations of 400, 500, and 600 ppm (n=7), respectively. The samples were immersed for seven days at 37oC in an incubator. The corrosion rate was tested using the weight-loss method and microstructure change was analyzed using a scanning electron microscope (SEM). Results: One-way ANOVA showed that there are significant differences in corrosion rates between beta titanium immersed in artificial saliva and beta titanium immersed in kiwi peel extract with p=0.01 (p<0.05). SEM analysis results showed that the group with the least surface changes was the one immersed in 400 ppm of kiwi peel extract. Conclusion: Weight-loss and SEM methods show similar results. Kiwi peel extract proved to decrease the corrosion rate and changes in the microstructure of the wire most effectively at a concentration of 400 ppm.
研究背景:已知β钛正畸丝具有良好的耐腐蚀性，但在酸性环境中较弱，从而加快了腐蚀速度。一种可以用来减少腐蚀的天然抑制剂是猕猴桃皮提取物，它具有很高的抗氧化水平。目的:考察提取液在酸性pH值(pH 5)下降低β钛腐蚀速率和微观结构变化的能力。方法:采用直径为0.016 x 0.022 in，长度为6 cm的β钛样品。28个样本(n=28)被分为4组，对照组浸泡在pH为5的人工唾液中，3个处理组浸泡在浓度为400、500和600 ppm的猕猴桃皮提取物中(n=7)。样品在37℃的培养箱中浸泡7天。用失重法测试腐蚀速率，用扫描电镜(SEM)分析微观组织变化。结果:单因素方差分析显示，人工唾液浸泡β钛与猕猴桃皮浸膏浸泡β钛的腐蚀速率有显著差异，p=0.01 (p<0.05)。扫描电镜分析结果表明，浸在400ppm的猕猴桃皮提取物中，其表面变化最小。结论:失重法和扫描电镜法结果相似。事实证明，400ppm浓度的猕猴桃皮提取物能最有效地降低金属丝的腐蚀速率和微观结构的变化。
{"title":"Changes in the corrosion rate and microstructure of beta titanium wire using kiwi peel extract","authors":"Hilda Fitria Lubis, Hanifa Natarisya","doi":"10.20473/j.djmkg.v56.i2.p122-126","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p122-126","url":null,"abstract":"Background: Beta titanium orthodontic wire is known to have good corrosion resistance but is weak in acidic environments, which advance the corrosion rate. One natural inhibitor that can be used to decrease corrosion is kiwi peel extract, which has a high antioxidant level. Purpose: This study aims to examine the ability of the extract to decrease the corrosion rate and microstructural changes of beta titanium at an acidic pH (pH 5). Methods: The samples used were beta titanium with a diameter of 0.016 x 0.022 in and a length of 6 cm. A total of 28 samples (n=28) were divided into four groups—a control group immersed in pH 5 artificial saliva and three treatment groups immersed in kiwi peel extract at concentrations of 400, 500, and 600 ppm (n=7), respectively. The samples were immersed for seven days at 37oC in an incubator. The corrosion rate was tested using the weight-loss method and microstructure change was analyzed using a scanning electron microscope (SEM). Results: One-way ANOVA showed that there are significant differences in corrosion rates between beta titanium immersed in artificial saliva and beta titanium immersed in kiwi peel extract with p=0.01 (p<0.05). SEM analysis results showed that the group with the least surface changes was the one immersed in 400 ppm of kiwi peel extract. Conclusion: Weight-loss and SEM methods show similar results. Kiwi peel extract proved to decrease the corrosion rate and changes in the microstructure of the wire most effectively at a concentration of 400 ppm.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"280 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72531517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.20473/j.djmkg.v56.i2.p132-138
N. Zubaidah, Sukaton, S. Kunarti, Meidi Kurnia Ariani, Dawailatur Rahman Setiady, D. M. Lashari
Background: The persistence of microorganisms in the root canal system is one of the leading causes of root canal treatment failure. Biofilms of putative pathogens hidden inside dentin tubules and other root canal ramifications may limit current disinfection protocols. Photodynamic therapy (PDT) with a wavelength of 628 nm can be used as an antimicrobial strategy that uses low-power laser energy to activate a non-toxic photosensitizer to produce singlet oxygen with the ability to kill microorganisms in root canals. Fusobacterium nucleatum was used because this bacterium is one of the bacteria involved in root canal infection. Purpose: The aim of this study was to compare the bactericidal efficacy of sodium hypochlorite (NaOCl) 2.5%, PDT, and a combination of PDT and NaOCl 2.5% against Fusobacterium nucleatum. Methods: Mature biofilm Fusobacterium nucleatum was divided into four groups according to the protocol of decontamination: K1 (negative control – biofilm), K2 (NaOCl 2.5%), K3 (PDT), and K4 (NaOCl 2.5% + PDT). Biofilm degradation was observed using optical density (OD) at 570 nm using a microplate reader. A reactive oxygen species quantity check was carried out using a nitroblue tetrazolium test, and OD observation was done with a microplate reader at 540 nm. Results: Group 4 (NaOCl 2.5% + PDT) showed more biofilm bacteria elimination than the other groups. Conclusion: A combination of PDT and NaOCl 2.5% can be considered an effective protocol for the elimination of Fusobacterium nucleatum. There is a potentiation relationship between NaOCl 2.5% and PDT FotoSan. Biofilm degradation occurs because of the effect of antibacterial NaOCl 2.5% and the irradiation effect of the Toluidine blue O photosensitizer.
{"title":"Degradation of Fusobacterium nucleatum biofilm and quantity of reactive oxygen species due to a combination of photodynamic therapy and 2.5% sodium hypochlorite","authors":"N. Zubaidah, Sukaton, S. Kunarti, Meidi Kurnia Ariani, Dawailatur Rahman Setiady, D. M. Lashari","doi":"10.20473/j.djmkg.v56.i2.p132-138","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p132-138","url":null,"abstract":"Background: The persistence of microorganisms in the root canal system is one of the leading causes of root canal treatment failure. Biofilms of putative pathogens hidden inside dentin tubules and other root canal ramifications may limit current disinfection protocols. Photodynamic therapy (PDT) with a wavelength of 628 nm can be used as an antimicrobial strategy that uses low-power laser energy to activate a non-toxic photosensitizer to produce singlet oxygen with the ability to kill microorganisms in root canals. Fusobacterium nucleatum was used because this bacterium is one of the bacteria involved in root canal infection. Purpose: The aim of this study was to compare the bactericidal efficacy of sodium hypochlorite (NaOCl) 2.5%, PDT, and a combination of PDT and NaOCl 2.5% against Fusobacterium nucleatum. Methods: Mature biofilm Fusobacterium nucleatum was divided into four groups according to the protocol of decontamination: K1 (negative control – biofilm), K2 (NaOCl 2.5%), K3 (PDT), and K4 (NaOCl 2.5% + PDT). Biofilm degradation was observed using optical density (OD) at 570 nm using a microplate reader. A reactive oxygen species quantity check was carried out using a nitroblue tetrazolium test, and OD observation was done with a microplate reader at 540 nm. Results: Group 4 (NaOCl 2.5% + PDT) showed more biofilm bacteria elimination than the other groups. Conclusion: A combination of PDT and NaOCl 2.5% can be considered an effective protocol for the elimination of Fusobacterium nucleatum. There is a potentiation relationship between NaOCl 2.5% and PDT FotoSan. Biofilm degradation occurs because of the effect of antibacterial NaOCl 2.5% and the irradiation effect of the Toluidine blue O photosensitizer.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85133809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.20473/j.djmkg.v56.i2.p80-86
Widiya Ulfa, Rosa Amalia, A. Santoso
Background: Tooth loss leads to decreased masticatory function, which affects nutritional intake. Tooth loss accompanied by poor nutritional status affects quality of life both physically and psychosocially. Purpose: This study was conducted to identify the relationship between number of teeth lost due to nutritional status and quality of life. Methods: A study conducted at Gulai Bancah Primary Health Care in Bukittinggi. Participants were recruited using a non-probability purposive sampling technique. 178 pre-elderlies aged between 45 and 59 years took part in this study. Tooth loss was reported in the dental examination format while nutritional status and quality of life pertaining to dental and oral health were determined using the Mini Nutritional Assessment (MNA) and the Geriatric Oral Health Assessment Index (GOHAI). Pearson correlation and path analysis were utilized to analyze the data. Results: The Pearson correlation test indicated a moderate correlation between tooth loss and nutritional status (r = -0.549) and a strong correlation between the number tooth lost and quality of life (r = -0.742). Pathway analysis confirmed that the direct effect value (beta coefficient = 0.552) was greater than the indirect effect value (beta coefficient = 0.189). The findings indicated that, indirectly, the amount of tooth loss mediated with nutritional status had no significant relationship to quality of life. Conclusion: An increase in tooth loss leads to pre-elderly poor nutritional status and quality of life. However, nutritional status is not an intermediate factor between the number of teeth lost and pre-elderly quality of life.
{"title":"The relationship between tooth loss and pre-elderly nutritional status and quality of life","authors":"Widiya Ulfa, Rosa Amalia, A. Santoso","doi":"10.20473/j.djmkg.v56.i2.p80-86","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p80-86","url":null,"abstract":"Background: Tooth loss leads to decreased masticatory function, which affects nutritional intake. Tooth loss accompanied by poor nutritional status affects quality of life both physically and psychosocially. Purpose: This study was conducted to identify the relationship between number of teeth lost due to nutritional status and quality of life. Methods: A study conducted at Gulai Bancah Primary Health Care in Bukittinggi. Participants were recruited using a non-probability purposive sampling technique. 178 pre-elderlies aged between 45 and 59 years took part in this study. Tooth loss was reported in the dental examination format while nutritional status and quality of life pertaining to dental and oral health were determined using the Mini Nutritional Assessment (MNA) and the Geriatric Oral Health Assessment Index (GOHAI). Pearson correlation and path analysis were utilized to analyze the data. Results: The Pearson correlation test indicated a moderate correlation between tooth loss and nutritional status (r = -0.549) and a strong correlation between the number tooth lost and quality of life (r = -0.742). Pathway analysis confirmed that the direct effect value (beta coefficient = 0.552) was greater than the indirect effect value (beta coefficient = 0.189). The findings indicated that, indirectly, the amount of tooth loss mediated with nutritional status had no significant relationship to quality of life. Conclusion: An increase in tooth loss leads to pre-elderly poor nutritional status and quality of life. However, nutritional status is not an intermediate factor between the number of teeth lost and pre-elderly quality of life.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78655072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.20473/j.djmkg.v56.i2.p87-91
B. F. Prakoeswa, Arofi Kurniawan, An’nisaa Chusida, B. N. Rizky, Anugerah I'zaaz Darmawan, Andini Kamilia Nur Aisyah, Aspalilah Alias
Background: The Demirjian method is used in assessing the stages of growth and development of teeth to calculate a person’s estimated age. In 1973, Demirjian identified the eight stages of tooth growth and development and their respective criteria. Purpose: To analyze the validity of Demirjian’s method for estimating dental age among children aged 6–17 years old in Surabaya, Indonesia. Methods: From August–October 2020, 162 panoramic radiographs of patients aged 6–17 years were taken at the radiology department of Airlangga Dental Hospital. Data analysis was conducted using SPSS software for different tests, including a paired t test. Results: Using the Demirjian method, there was no significant dissimilarity between chronological age (CA) and estimated dental age (EDA) in the male group. However, a significant dissimilarity was found between CA and EDA in the female group. Conclusion: Demirjian’s method can be used as a tool for estimating the dental age of males age 6-17 years old in Surabaya.
{"title":"Using the Demirjian method for estimating the dental age of children in Surabaya, Indonesia","authors":"B. F. Prakoeswa, Arofi Kurniawan, An’nisaa Chusida, B. N. Rizky, Anugerah I'zaaz Darmawan, Andini Kamilia Nur Aisyah, Aspalilah Alias","doi":"10.20473/j.djmkg.v56.i2.p87-91","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p87-91","url":null,"abstract":"Background: The Demirjian method is used in assessing the stages of growth and development of teeth to calculate a person’s estimated age. In 1973, Demirjian identified the eight stages of tooth growth and development and their respective criteria. Purpose: To analyze the validity of Demirjian’s method for estimating dental age among children aged 6–17 years old in Surabaya, Indonesia. Methods: From August–October 2020, 162 panoramic radiographs of patients aged 6–17 years were taken at the radiology department of Airlangga Dental Hospital. Data analysis was conducted using SPSS software for different tests, including a paired t test. Results: Using the Demirjian method, there was no significant dissimilarity between chronological age (CA) and estimated dental age (EDA) in the male group. However, a significant dissimilarity was found between CA and EDA in the female group. Conclusion: Demirjian’s method can be used as a tool for estimating the dental age of males age 6-17 years old in Surabaya.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79997541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.20473/j.djmkg.v56.i2.p104-108
Michael Josef Kridanto Kamadjaja, Sherman Salim, Wiwik Herawati Waluyo, T. Noor
Background: Bone resorption due to tooth extraction leads to unpredictable bone volume for future prosthetics. Crab shells were promoted as a solution to prevent bone resorption, along with an effort to reduce biological waste. Purpose: This study aimed to analyze the expression of bone sialoprotein (BSP) and dentine matrix protein-1 (DMP1) in the wound healing process in tooth-extraction sockets after applying a crab shell-derived hydroxyapatite scaffold. Methods: The subjects (28 Cavia cobaya) were divided into control and treatment groups. The control group was left untreated, while the treatment group received a hydroxyapatite scaffold of Portunus pelagicus shell in the tooth socket. The expression of BSP and DMP1 was determined by immunohistochemical staining on days 7 and 14. One-way analysis of variance and Tukey’s honest significance difference test were used to find the groups with the most significant difference. Results: The highest mean expression of BSP and DMP1 was in the day 14 treatment group, while the lowest was in the day 7 control group. Conclusion: Administering hydroxyapatite scaffold derived from the Portunus pelagicus shell to the post-extraction sockets increased the expression of both BSP and DMP1.
{"title":"Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation","authors":"Michael Josef Kridanto Kamadjaja, Sherman Salim, Wiwik Herawati Waluyo, T. Noor","doi":"10.20473/j.djmkg.v56.i2.p104-108","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p104-108","url":null,"abstract":"Background: Bone resorption due to tooth extraction leads to unpredictable bone volume for future prosthetics. Crab shells were promoted as a solution to prevent bone resorption, along with an effort to reduce biological waste. Purpose: This study aimed to analyze the expression of bone sialoprotein (BSP) and dentine matrix protein-1 (DMP1) in the wound healing process in tooth-extraction sockets after applying a crab shell-derived hydroxyapatite scaffold. Methods: The subjects (28 Cavia cobaya) were divided into control and treatment groups. The control group was left untreated, while the treatment group received a hydroxyapatite scaffold of Portunus pelagicus shell in the tooth socket. The expression of BSP and DMP1 was determined by immunohistochemical staining on days 7 and 14. One-way analysis of variance and Tukey’s honest significance difference test were used to find the groups with the most significant difference. Results: The highest mean expression of BSP and DMP1 was in the day 14 treatment group, while the lowest was in the day 7 control group. Conclusion: Administering hydroxyapatite scaffold derived from the Portunus pelagicus shell to the post-extraction sockets increased the expression of both BSP and DMP1.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73504916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.20473/j.djmkg.v56.i2.p92-97
Martina Amalia, Priscillia Sekar Yosuana, Iqlima Salsabila binti Mohammad, Filya Suri Risky Nababan, Zulkarnain, Pitu Wulandari, A. Nasution, Armia Syahputra
Background: Andaliman (Zanthoxylum achantopodium DC) is an endemic plant that is found in the province of Sumatera Utara, Indonesia. It contains secondary metabolites, such as alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids/steroids, which can potentially be used as a mouthwash. Streptococcus sanguinis and Staphylococcus aureus are the primary colonizing bacteria in plaque formation. Bacterial plaque is known to be the main cause of periodontal disease but can be controlled mechanically and chemically using mouthwash. Purpose: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of andaliman extract mouthwash (2%, 4%, 8%) against Streptococcus sanguinis ATCC®10556™ and Staphylococcus aureus ATCC® 25923™. Methods: This is a laboratory study with a post-test control-only design. The sample consists of andaliman extract mouthwash (2%, 4%, 8%), a positive control (chlorhexidine gluconate 0.2%), and a negative control (mouthwash formulation without andaliman extract) with three repetitions for each group. Data were analyzed with the one-way ANOVA test and post hoc LSD test. Results: The andaliman extract mouthwash with concentrations of 2%, 4%, and 8% significantly reduced the number of Streptococcus sanguinis and Staphylococcus aureus colonies (p<0.05), and there was a significant difference in the andaliman extract mouthwash with concentrations of 2%, 4%, and 8% compared to the negative control. Conclusion: Andaliman extract mouthwash with a concentration of 8% was more effective in inhibiting Streptococcus sanguinis growth than Staphylococcus aureus. The MIC values for both bacteria were 2%, but the study could not determine the MBC value.
{"title":"Inhibitory test of andaliman (Zanthoxylum achantopodium DC) extract mouthwash against dental plaque bacteria","authors":"Martina Amalia, Priscillia Sekar Yosuana, Iqlima Salsabila binti Mohammad, Filya Suri Risky Nababan, Zulkarnain, Pitu Wulandari, A. Nasution, Armia Syahputra","doi":"10.20473/j.djmkg.v56.i2.p92-97","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p92-97","url":null,"abstract":"Background: Andaliman (Zanthoxylum achantopodium DC) is an endemic plant that is found in the province of Sumatera Utara, Indonesia. It contains secondary metabolites, such as alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids/steroids, which can potentially be used as a mouthwash. Streptococcus sanguinis and Staphylococcus aureus are the primary colonizing bacteria in plaque formation. Bacterial plaque is known to be the main cause of periodontal disease but can be controlled mechanically and chemically using mouthwash. Purpose: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of andaliman extract mouthwash (2%, 4%, 8%) against Streptococcus sanguinis ATCC®10556™ and Staphylococcus aureus ATCC® 25923™. Methods: This is a laboratory study with a post-test control-only design. The sample consists of andaliman extract mouthwash (2%, 4%, 8%), a positive control (chlorhexidine gluconate 0.2%), and a negative control (mouthwash formulation without andaliman extract) with three repetitions for each group. Data were analyzed with the one-way ANOVA test and post hoc LSD test. Results: The andaliman extract mouthwash with concentrations of 2%, 4%, and 8% significantly reduced the number of Streptococcus sanguinis and Staphylococcus aureus colonies (p<0.05), and there was a significant difference in the andaliman extract mouthwash with concentrations of 2%, 4%, and 8% compared to the negative control. Conclusion: Andaliman extract mouthwash with a concentration of 8% was more effective in inhibiting Streptococcus sanguinis growth than Staphylococcus aureus. The MIC values for both bacteria were 2%, but the study could not determine the MBC value.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90842213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.20473/j.djmkg.v56.i2.p98-103
S. Suhartono, C. Soraya, Putri Shabira
Background: Enterococcus faecalis commonly infects root canals by forming a biofilm. Extracts from neem leaves (Azadirachta indica A. Juss) have been shown to have antibacterial properties, indicating their potential in preventing or treating biofilm formation caused by bacteria. Purpose: This study aims to investigate the phytochemical compounds present in neem leaves (Azadirachta indica A. Juss) and establish the concentration of ethanol-based neem leaf extract that can effectively inhibit the in vitro growth of Enterococcus faecalis biofilm. Methods: This study employed the maceration technique for extraction, gas chromatography mass spectroscopy for the analysis of plant chemicals, and a microtiter plate assay for measuring biofilm formation with treatment concentrations of 6.25%, 12.5%, 25%, 50%, and 75%, with a positive control of 0.2% chlorhexidine. Results: A phytochemical analysis revealed that the ethanol extract of neem leaves contained 22 different metabolites, mainly terpenoids and fatty acids. The extract demonstrated antibiofilm activity only at a concentration of 12.5% with an average biofilm inhibition of 36.85%. However, lower concentrations of 6.25%, 25%, 50%, and 75% had the opposite effect, promoting biofilm formation in Enterococcus faecalis. Conclusion: Phytochemical metabolite contained in the ethanolic extracts of neem leaves might contribute a promising agent in treating a biofilm-mediated root canal infection of Enterococcus faecalis.
背景:粪肠球菌通常通过形成生物膜感染根管。印楝叶提取物(印楝叶)已被证明具有抗菌特性，表明其在预防或治疗细菌引起的生物膜形成方面的潜力。目的:研究印楝叶(Azadirachta indica A. Juss)中的植物化学成分，建立能有效抑制粪肠球菌生物膜体外生长的乙醇基印楝叶提取物的浓度。方法:采用浸渍法提取，气相色谱质谱法分析植物化学成分，微滴板法测定生物膜形成，处理浓度分别为6.25%、12.5%、25%、50%和75%，阳性对照为0.2%氯己定。结果:经植物化学分析，印楝叶乙醇提取物含有22种不同的代谢产物，主要是萜类和脂肪酸。该提取物仅在浓度为12.5%时表现出抗膜活性，平均生物膜抑制率为36.85%。然而，6.25%、25%、50%和75%的较低浓度会产生相反的效果，促进粪肠球菌生物膜的形成。结论:印楝叶乙醇提取物中含有的植物化学代谢物可能是治疗生物膜介导的粪肠球菌根管感染的一种有前景的药物。
{"title":"Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro","authors":"S. Suhartono, C. Soraya, Putri Shabira","doi":"10.20473/j.djmkg.v56.i2.p98-103","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p98-103","url":null,"abstract":"Background: Enterococcus faecalis commonly infects root canals by forming a biofilm. Extracts from neem leaves (Azadirachta indica A. Juss) have been shown to have antibacterial properties, indicating their potential in preventing or treating biofilm formation caused by bacteria. Purpose: This study aims to investigate the phytochemical compounds present in neem leaves (Azadirachta indica A. Juss) and establish the concentration of ethanol-based neem leaf extract that can effectively inhibit the in vitro growth of Enterococcus faecalis biofilm. Methods: This study employed the maceration technique for extraction, gas chromatography mass spectroscopy for the analysis of plant chemicals, and a microtiter plate assay for measuring biofilm formation with treatment concentrations of 6.25%, 12.5%, 25%, 50%, and 75%, with a positive control of 0.2% chlorhexidine. Results: A phytochemical analysis revealed that the ethanol extract of neem leaves contained 22 different metabolites, mainly terpenoids and fatty acids. The extract demonstrated antibiofilm activity only at a concentration of 12.5% with an average biofilm inhibition of 36.85%. However, lower concentrations of 6.25%, 25%, 50%, and 75% had the opposite effect, promoting biofilm formation in Enterococcus faecalis. Conclusion: Phytochemical metabolite contained in the ethanolic extracts of neem leaves might contribute a promising agent in treating a biofilm-mediated root canal infection of Enterococcus faecalis.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80359656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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