首页 > 最新文献

Developmental cell最新文献

英文 中文
Gene module reconstruction identifies cellular differentiation processes and the regulatory logic of specialized secretion in zebrafish 基因模块重构确定了斑马鱼的细胞分化过程和特化分泌的调控逻辑
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-25 DOI: 10.1016/j.devcel.2024.10.015
Yiqun Wang, Jialin Liu, Lucia Y. Du, Jannik L. Wyss, Jeffrey A. Farrell, Alexander F. Schier
During differentiation, cells become structurally and functionally specialized, but comprehensive views of the underlying remodeling processes are elusive. Here, we leverage single-cell RNA sequencing (scRNA-seq) developmental trajectories to reconstruct differentiation using two secretory tissues as models—the zebrafish notochord and hatching gland. First, we integrated expression and functional similarities to identify gene modules, revealing dozens of modules representing known and newly associated differentiation processes and their dynamics. Second, we focused on the unfolded protein response (UPR) transducer module to study how general versus cell-type-specific secretory functions are regulated. Profiling loss- and gain-of-function embryos identified that the UPR transcription factors creb3l1, creb3l2, and xbp1 are master regulators of a general secretion program. creb3l1/creb3l2 additionally activate an extracellular matrix secretion program, while xbp1 partners with bhlha15 to activate a gland-like secretion program. Our study presents module identification via multi-source integration for reconstructing differentiation (MIMIR) and illustrates how transcription factors confer general and specialized cellular functions.
在分化过程中,细胞会在结构上和功能上变得特化,但人们对其背后的重塑过程却缺乏全面的了解。在这里,我们利用单细胞 RNA 测序(scRNA-seq)发育轨迹,以两种分泌组织--斑马鱼脊索和孵化腺--为模型重建分化。首先,我们整合了表达和功能相似性来识别基因模块,发现了数十个代表已知和新相关分化过程及其动态的模块。其次,我们重点研究了未折叠蛋白反应(UPR)转导模块,以研究一般分泌功能与细胞类型特异性分泌功能是如何调控的。对功能缺失和功能增益胚胎的剖析发现,UPR转录因子creb3l1、creb3l2和xbp1是一般分泌程序的主调控因子,creb3l1/creb3l2还能激活细胞外基质分泌程序,而xbp1则与bhlha15合作激活腺体样分泌程序。我们的研究通过多源整合重构分化(MIMIR)进行了模块识别,并说明了转录因子如何赋予细胞一般和特化功能。
{"title":"Gene module reconstruction identifies cellular differentiation processes and the regulatory logic of specialized secretion in zebrafish","authors":"Yiqun Wang, Jialin Liu, Lucia Y. Du, Jannik L. Wyss, Jeffrey A. Farrell, Alexander F. Schier","doi":"10.1016/j.devcel.2024.10.015","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.015","url":null,"abstract":"During differentiation, cells become structurally and functionally specialized, but comprehensive views of the underlying remodeling processes are elusive. Here, we leverage single-cell RNA sequencing (scRNA-seq) developmental trajectories to reconstruct differentiation using two secretory tissues as models—the zebrafish notochord and hatching gland. First, we integrated expression and functional similarities to identify gene modules, revealing dozens of modules representing known and newly associated differentiation processes and their dynamics. Second, we focused on the unfolded protein response (UPR) transducer module to study how general versus cell-type-specific secretory functions are regulated. Profiling loss- and gain-of-function embryos identified that the UPR transcription factors <em>creb3l1</em>, <em>creb3l2</em>, and <em>xbp1</em> are master regulators of a general secretion program. <em>creb3l1/creb3l2</em> additionally activate an extracellular matrix secretion program, while <em>xbp1</em> partners with <em>bhlha15</em> to activate a gland-like secretion program. Our study presents module identification via multi-source integration for reconstructing differentiation (MIMIR) and illustrates how transcription factors confer general and specialized cellular functions.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"19 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142713102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of an inducible DNA barcoding system to understand lineage changes in Arabidopsis regeneration 开发可诱导的 DNA 条形码系统,了解拟南芥再生过程中的品系变化
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-25 DOI: 10.1016/j.devcel.2024.10.023
Xinyue Lu, Qiyan Zhang, Zejia Wang, Xuanzhi Cheng, Huiru Yan, Shuyi Cai, Huawei Zhang, Qikun Liu
Plants demonstrate a high degree of developmental plasticity, capable of regenerating entire individuals from detached somatic tissues—a regenerative phenomenon rarely observed in metazoa. Consequently, elucidating the lineage relationship between somatic founder cells and descendant cells in regenerated plant organs has long been a pursuit. In this study, we developed and optimized both DNA barcode- and multi-fluorescence-based cell-lineage tracing toolsets, employing an inducible method to mark individual cells in Arabidopsis donor somatic tissues at the onset of regeneration. Utilizing these complementary methods, we scrutinized cell identities at the single-cell level and presented compelling evidence that all cells in the regenerated Arabidopsis plants, irrespective of their organ types, originated from a single progenitor cell in the donor somatic tissue. Our discovery suggests a single-cell passage directing the transition from multicellular donor tissue to regenerated plants, thereby creating opportunities for cell-cell competition during plant regeneration—a strategy for maximizing survival.
植物具有高度的发育可塑性,能够从分离的体细胞组织再生出完整的个体--这种再生现象在中生代动物中很少见。因此,阐明再生植物器官中体细胞创始细胞和后代细胞之间的世系关系一直是人们追求的目标。在本研究中,我们开发并优化了基于 DNA 条形码和多重荧光的细胞系追踪工具集,采用诱导方法在再生开始时标记拟南芥供体体细胞组织中的单个细胞。利用这些互补方法,我们在单细胞水平上仔细研究了细胞特性,并提出了令人信服的证据,证明再生拟南芥植株中的所有细胞,无论其器官类型如何,都源自供体体细胞中的单个祖细胞。我们的发现表明,单细胞通道引导着从多细胞供体组织到再生植物的过渡,从而为植物再生过程中的细胞竞争创造了机会--这是一种最大限度提高存活率的策略。
{"title":"Development of an inducible DNA barcoding system to understand lineage changes in Arabidopsis regeneration","authors":"Xinyue Lu, Qiyan Zhang, Zejia Wang, Xuanzhi Cheng, Huiru Yan, Shuyi Cai, Huawei Zhang, Qikun Liu","doi":"10.1016/j.devcel.2024.10.023","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.023","url":null,"abstract":"Plants demonstrate a high degree of developmental plasticity, capable of regenerating entire individuals from detached somatic tissues—a regenerative phenomenon rarely observed in metazoa. Consequently, elucidating the lineage relationship between somatic founder cells and descendant cells in regenerated plant organs has long been a pursuit. In this study, we developed and optimized both DNA barcode- and multi-fluorescence-based cell-lineage tracing toolsets, employing an inducible method to mark individual cells in <em>Arabidopsis</em> donor somatic tissues at the onset of regeneration. Utilizing these complementary methods, we scrutinized cell identities at the single-cell level and presented compelling evidence that all cells in the regenerated <em>Arabidopsis</em> plants, irrespective of their organ types, originated from a single progenitor cell in the donor somatic tissue. Our discovery suggests a single-cell passage directing the transition from multicellular donor tissue to regenerated plants, thereby creating opportunities for cell-cell competition during plant regeneration—a strategy for maximizing survival.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"8 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FZD5 controls intestinal crypt homeostasis and colonic Wnt surrogate agonist response FZD5控制肠隐窝稳态和结肠Wnt替代激动剂反应
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-22 DOI: 10.1016/j.devcel.2024.10.022
Qinghui Mu, Andrew Ha, Antonio J.M. Santos, Yuan-Hung Lo, Vincent van Unen, Yi Miao, Madeline Tomaske, Veronica K. Guzman, Samira Alwahabi, Jenny J. Yuan, Lu Deng, Linheng Li, K. Christopher Garcia, Calvin J. Kuo
The rapidly regenerating intestinal epithelium requires crypt intestinal stem cells (ISCs). Wnt/β-catenin signaling maintains crypt homeostasis and Lgr5+ ISCs, and WNT ligands bind Frizzled receptors (FZD1–10). Identifying specific FZD(s) essential for intestinal homeostasis has been elusive; however, bioengineered antagonists blocking Wnt binding to FZD5 and FZD8 deplete the gut epithelium in vivo, highlighting potential roles. Here, an epithelial-specific Fzd5 knockout (KO) elicited lethal pan-intestinal crypt and villus loss, whereas an Lgr5+ ISC-specific Fzd5 KO depleted Lgr5+ ISCs via premature differentiation and repressed Wnt target genes. Fzd5-null phenotypes were rescued by constitutive β-catenin activation in vivo and in both mouse and human enteroids. KO of Fzd5, not Fzd8, in enteroids ablated responsiveness to dual-specificity FZD5/FZD8-selective Wnt surrogate agonists, which ameliorated DSS-induced colitis in wild-type and Fzd8 KO mice. Overall, FZD5 is a dominant and essential regulator of crypt homeostasis, Lgr5+ ISCs, and intestinal response to Wnt surrogate agonists, with implications for therapeutic mucosal repair.
快速再生的肠上皮需要隐窝肠干细胞(ISC)。Wnt/β-catenin信号维持隐窝稳态和Lgr5+ ISC,WNT配体与Frizzled受体(FZD1-10)结合。然而,阻断 Wnt 与 FZD5 和 FZD8 结合的生物工程拮抗剂会使体内肠道上皮细胞衰竭,从而突显出其潜在的作用。在这里,上皮特异性Fzd5基因敲除(KO)引起了致命的泛肠道隐窝和绒毛缺失,而Lgr5+ ISC特异性Fzd5基因敲除则通过过早分化和抑制Wnt靶基因耗竭了Lgr5+ ISC。Fzd5缺失的表型在体内以及小鼠和人类肠道中都能通过组成型β-catenin激活得到挽救。在肠道中 KO Fzd5(而非 Fzd8)会减弱对 FZD5/FZD8 选择性 Wnt 替代激动剂的反应,从而改善野生型和 Fzd8 KO 小鼠 DSS 诱导的结肠炎。总之,FZD5是隐窝稳态、Lgr5+ ISC和肠道对Wnt替代激动剂反应的主要和基本调节因子,对治疗性粘膜修复具有重要意义。
{"title":"FZD5 controls intestinal crypt homeostasis and colonic Wnt surrogate agonist response","authors":"Qinghui Mu, Andrew Ha, Antonio J.M. Santos, Yuan-Hung Lo, Vincent van Unen, Yi Miao, Madeline Tomaske, Veronica K. Guzman, Samira Alwahabi, Jenny J. Yuan, Lu Deng, Linheng Li, K. Christopher Garcia, Calvin J. Kuo","doi":"10.1016/j.devcel.2024.10.022","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.022","url":null,"abstract":"The rapidly regenerating intestinal epithelium requires crypt intestinal stem cells (ISCs). Wnt/β-catenin signaling maintains crypt homeostasis and Lgr5+ ISCs, and WNT ligands bind Frizzled receptors (FZD1–10). Identifying specific FZD(s) essential for intestinal homeostasis has been elusive; however, bioengineered antagonists blocking Wnt binding to FZD5 and FZD8 deplete the gut epithelium <em>in vivo</em>, highlighting potential roles. Here, an epithelial-specific <em>Fzd5</em> knockout (KO) elicited lethal pan-intestinal crypt and villus loss, whereas an Lgr5+ ISC-specific <em>Fzd5</em> KO depleted Lgr5+ ISCs via premature differentiation and repressed Wnt target genes. Fzd5-null phenotypes were rescued by constitutive β-catenin activation <em>in vivo</em> and in both mouse and human enteroids. KO of <em>Fzd5</em>, not <em>Fzd8</em>, in enteroids ablated responsiveness to dual-specificity FZD5/FZD8-selective Wnt surrogate agonists, which ameliorated DSS-induced colitis in wild-type and <em>Fzd8</em> KO mice. Overall, FZD5 is a dominant and essential regulator of crypt homeostasis, Lgr5+ ISCs, and intestinal response to Wnt surrogate agonists, with implications for therapeutic mucosal repair.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"23 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Frizzled5 controls murine intestinal epithelial cell plasticity through organization of chromatin accessibility Frizzled5通过组织染色质可及性控制小鼠肠上皮细胞的可塑性
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-22 DOI: 10.1016/j.devcel.2024.10.021
Lu Deng, Xi C. He, Shiyuan Chen, Ning Zhang, Fengyan Deng, Allison Scott, Yanfeng He, Dai Tsuchiya, Sarah E. Smith, Michael Epp, Seth Malloy, Fang Liu, Mark Hembree, Qinghui Mu, Jeffrey S. Haug, Ermanno Malagola, Huzaifa Hassan, Kaitlyn Petentler, Rhonda Egidy, Lucinda Maddera, Linheng Li
The homeostasis of the intestinal epithelium relies on intricate yet insufficiently understood mechanisms of intestinal epithelial plasticity. Here, we elucidate the pivotal role of Frizzled5 (Fzd5), a Wnt pathway receptor, as a determinant of murine intestinal epithelial cell fate. Deletion of Fzd5 in Lgr5+ intestinal stem cells (ISCs) impairs their self-renewal, whereas its deletion in Krt19+ cells disrupts lineage generation, without affecting crypt integrity in either case. However, a broader deletion of Fzd5 across the epithelium leads to substantial crypt deterioration. Integrated analysis of single-cell RNA sequencing (scRNA-seq) and single-cell ATAC-seq (scATAC-seq) identifies that Fzd5 governs chromatin accessibility, orchestrating the regulation of stem- and lineage-related gene expression mainly in ISCs and progenitor cells. In summary, our findings provide insights into the regulatory role of Fzd5 in governing intestinal epithelial plasticity.
肠上皮细胞的稳态依赖于错综复杂的肠上皮细胞可塑性机制,但人们对这种机制的了解还不够。在这里,我们阐明了Wnt通路受体Frizzled5(Fzd5)作为小鼠肠上皮细胞命运决定因素的关键作用。在Lgr5+肠干细胞(ISCs)中缺失Fzd5会影响它们的自我更新,而在Krt19+细胞中缺失Fzd5则会破坏细胞系的生成,但两种情况都不会影响隐窝的完整性。然而,在上皮细胞中更广泛地缺失 Fzd5 会导致隐窝严重恶化。单细胞RNA测序(scRNA-seq)和单细胞ATAC-seq(scATAC-seq)的综合分析表明,Fzd5控制染色质的可及性,主要在ISC和祖细胞中协调调控干系和系谱相关基因的表达。总之,我们的研究结果为了解 Fzd5 在肠上皮可塑性中的调控作用提供了深入的见解。
{"title":"Frizzled5 controls murine intestinal epithelial cell plasticity through organization of chromatin accessibility","authors":"Lu Deng, Xi C. He, Shiyuan Chen, Ning Zhang, Fengyan Deng, Allison Scott, Yanfeng He, Dai Tsuchiya, Sarah E. Smith, Michael Epp, Seth Malloy, Fang Liu, Mark Hembree, Qinghui Mu, Jeffrey S. Haug, Ermanno Malagola, Huzaifa Hassan, Kaitlyn Petentler, Rhonda Egidy, Lucinda Maddera, Linheng Li","doi":"10.1016/j.devcel.2024.10.021","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.021","url":null,"abstract":"The homeostasis of the intestinal epithelium relies on intricate yet insufficiently understood mechanisms of intestinal epithelial plasticity. Here, we elucidate the pivotal role of Frizzled5 (Fzd5), a Wnt pathway receptor, as a determinant of murine intestinal epithelial cell fate. Deletion of Fzd5 in Lgr5<sup>+</sup> intestinal stem cells (ISCs) impairs their self-renewal, whereas its deletion in Krt19<sup>+</sup> cells disrupts lineage generation, without affecting crypt integrity in either case. However, a broader deletion of Fzd5 across the epithelium leads to substantial crypt deterioration. Integrated analysis of single-cell RNA sequencing (scRNA-seq) and single-cell ATAC-seq (scATAC-seq) identifies that Fzd5 governs chromatin accessibility, orchestrating the regulation of stem- and lineage-related gene expression mainly in ISCs and progenitor cells. In summary, our findings provide insights into the regulatory role of Fzd5 in governing intestinal epithelial plasticity.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"254 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
tRNA-m1A methylation controls the infection of Magnaporthe oryzae by supporting ergosterol biosynthesis. tRNA-m1A 甲基化通过支持麦角甾醇的生物合成来控制木格氏球菌的感染。
IF 10.7 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-18 Epub Date: 2024-08-26 DOI: 10.1016/j.devcel.2024.08.002
Rongrong He, Ziwei Lv, Yinan Li, Shuchao Ren, Jiaqi Cao, Jun Zhu, Xinrong Zhang, Huimin Wu, Lihao Wan, Ji Tang, Shutong Xu, Xiao-Lin Chen, Zhipeng Zhou

Ergosterols are essential components of fungal plasma membranes. Inhibitors targeting ergosterol biosynthesis (ERG) genes are critical for controlling fungal pathogens, including Magnaporthe oryzae, the fungus that causes rice blast. However, the translational mechanisms governing ERG gene expression remain largely unexplored. Here, we show that the Trm6/Trm61 complex catalyzes dynamic N1-methyladenosine at position 58 (m1A58) in 51 transfer RNAs (tRNAs) of M. oryzae, significantly influencing translation at both the initiation and elongation stages. Notably, tRNA m1A58 promotes elongation speed at most cognate codons mainly by enhancing eEF1-tRNA binding rather than affecting tRNA abundance or charging. The absence of m1A58 leads to substantial decreases in the translation of ERG genes, ergosterol production, and, consequently, fungal virulence. Simultaneously targeting the Trm6/Trm61 complex and the ergosterol biosynthesis pathway markedly improves rice blast control. Our findings demonstrate an important role of m1A58-mediated translational regulation in ergosterol production and fungal infection, offering a potential strategy for fungicide development.

麦角甾醇是真菌质膜的重要组成部分。针对麦角甾醇生物合成(ERG)基因的抑制剂对于控制真菌病原体(包括导致稻瘟病的真菌 Magnaporthe oryzae)至关重要。然而,ERG 基因表达的翻译机制在很大程度上仍未得到探索。在这里,我们发现,Trm6/Trm61 复合物能催化 M. oryzae 51 种转运 RNA(tRNA)中位于 58 位的 N1-甲基腺苷(m1A58)的动态变化,从而显著影响起始和延伸阶段的翻译。值得注意的是,tRNA m1A58 主要通过增强 eEF1 与 tRNA 的结合而不是影响 tRNA 的丰度或充电来促进大多数同源密码子的延伸速度。m1A58 的缺失会导致 ERG 基因的翻译和麦角甾醇的产生大幅减少,进而影响真菌的毒力。同时靶向 Trm6/Trm61 复合物和麦角甾醇生物合成途径可显著改善稻瘟病的防治效果。我们的研究结果证明了 m1A58 介导的翻译调控在麦角甾醇生产和真菌感染中的重要作用,为杀真菌剂的开发提供了一种潜在的策略。
{"title":"tRNA-m<sup>1</sup>A methylation controls the infection of Magnaporthe oryzae by supporting ergosterol biosynthesis.","authors":"Rongrong He, Ziwei Lv, Yinan Li, Shuchao Ren, Jiaqi Cao, Jun Zhu, Xinrong Zhang, Huimin Wu, Lihao Wan, Ji Tang, Shutong Xu, Xiao-Lin Chen, Zhipeng Zhou","doi":"10.1016/j.devcel.2024.08.002","DOIUrl":"10.1016/j.devcel.2024.08.002","url":null,"abstract":"<p><p>Ergosterols are essential components of fungal plasma membranes. Inhibitors targeting ergosterol biosynthesis (ERG) genes are critical for controlling fungal pathogens, including Magnaporthe oryzae, the fungus that causes rice blast. However, the translational mechanisms governing ERG gene expression remain largely unexplored. Here, we show that the Trm6/Trm61 complex catalyzes dynamic N<sup>1</sup>-methyladenosine at position 58 (m<sup>1</sup>A58) in 51 transfer RNAs (tRNAs) of M. oryzae, significantly influencing translation at both the initiation and elongation stages. Notably, tRNA m<sup>1</sup>A58 promotes elongation speed at most cognate codons mainly by enhancing eEF1-tRNA binding rather than affecting tRNA abundance or charging. The absence of m<sup>1</sup>A58 leads to substantial decreases in the translation of ERG genes, ergosterol production, and, consequently, fungal virulence. Simultaneously targeting the Trm6/Trm61 complex and the ergosterol biosynthesis pathway markedly improves rice blast control. Our findings demonstrate an important role of m<sup>1</sup>A58-mediated translational regulation in ergosterol production and fungal infection, offering a potential strategy for fungicide development.</p>","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"2931-2946.e7"},"PeriodicalIF":10.7,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway. 细胞周期通过激活augmin途径控制拟南芥的纺锤体结构。
IF 10.7 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-18 Epub Date: 2024-08-26 DOI: 10.1016/j.devcel.2024.08.001
Mariana Romeiro Motta, François Nédélec, Helen Saville, Elke Woelken, Claire Jacquerie, Martine Pastuglia, Sara Christina Stolze, Eveline Van De Slijke, Lev Böttger, Katia Belcram, Hirofumi Nakagami, Geert De Jaeger, David Bouchez, Arp Schnittger

To ensure an even segregation of chromosomes during somatic cell division, eukaryotes rely on mitotic spindles. Here, we measured prime characteristics of the Arabidopsis mitotic spindle and built a three-dimensional dynamic model using Cytosim. We identified the cell-cycle regulator CYCLIN-DEPENDENT KINASE B1 (CDKB1) together with its cyclin partner CYCB3;1 as key regulators of spindle morphology in Arabidopsis. We found that the augmin component ENDOSPERM DEFECTIVE1 (EDE1) is a substrate of the CDKB1;1-CYCB3;1 complex. A non-phosphorylatable mutant rescue of ede1 resembled the spindle phenotypes of cycb3;1 and cdkb1 mutants and the protein associated less efficiently with spindle microtubules. Accordingly, reducing the level of augmin in simulations recapitulated the phenotypes observed in the mutants. Our findings emphasize the importance of cell-cycle-dependent phospho-control of the mitotic spindle in plant cells and support the validity of our model as a framework for the exploration of mechanisms controlling the organization of the eukaryotic spindle.

为了确保体细胞分裂过程中染色体的均匀分离,真核生物依赖于有丝分裂纺锤体。在这里,我们测量了拟南芥有丝分裂纺锤体的主要特征,并利用 Cytosim 建立了一个三维动态模型。我们发现细胞周期调节因子 CYCLIN-DEPENDENT KINASE B1 (CDKB1) 及其细胞周期蛋白伙伴 CYCB3;1 是拟南芥纺锤体形态的关键调节因子。我们发现,增强子成分ENDOSPERM DEFECTIVE1(EDE1)是CDKB1;1-CYCB3;1复合物的底物。ede1的非磷酸化突变体与cycb3;1和CDKB1突变体的纺锤体表型相似,而且该蛋白与纺锤体微管的结合效率较低。因此,在模拟中降低蛟龙蛋白的水平可以重现突变体中观察到的表型。我们的发现强调了植物细胞中依赖细胞周期的有丝分裂纺锤体磷酸化控制的重要性,并支持我们的模型作为探索真核生物纺锤体组织控制机制框架的有效性。
{"title":"The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway.","authors":"Mariana Romeiro Motta, François Nédélec, Helen Saville, Elke Woelken, Claire Jacquerie, Martine Pastuglia, Sara Christina Stolze, Eveline Van De Slijke, Lev Böttger, Katia Belcram, Hirofumi Nakagami, Geert De Jaeger, David Bouchez, Arp Schnittger","doi":"10.1016/j.devcel.2024.08.001","DOIUrl":"10.1016/j.devcel.2024.08.001","url":null,"abstract":"<p><p>To ensure an even segregation of chromosomes during somatic cell division, eukaryotes rely on mitotic spindles. Here, we measured prime characteristics of the Arabidopsis mitotic spindle and built a three-dimensional dynamic model using Cytosim. We identified the cell-cycle regulator CYCLIN-DEPENDENT KINASE B1 (CDKB1) together with its cyclin partner CYCB3;1 as key regulators of spindle morphology in Arabidopsis. We found that the augmin component ENDOSPERM DEFECTIVE1 (EDE1) is a substrate of the CDKB1;1-CYCB3;1 complex. A non-phosphorylatable mutant rescue of ede1 resembled the spindle phenotypes of cycb3;1 and cdkb1 mutants and the protein associated less efficiently with spindle microtubules. Accordingly, reducing the level of augmin in simulations recapitulated the phenotypes observed in the mutants. Our findings emphasize the importance of cell-cycle-dependent phospho-control of the mitotic spindle in plant cells and support the validity of our model as a framework for the exploration of mechanisms controlling the organization of the eukaryotic spindle.</p>","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"2947-2961.e9"},"PeriodicalIF":10.7,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lysosomal catabolic activity promotes the exit of murine totipotent 2-cell state by silencing early-embryonic retrotransposons 溶酶体分解代谢活动通过沉默早期胚胎逆转录载体促进小鼠脱离全能2细胞状态
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-18 DOI: 10.1016/j.devcel.2024.10.018
Hao Wu, Lanrui Cao, Xinpeng Wen, Jiawei Fan, Yuan Wang, Heyong Hu, Shuyan Ji, Yinli Zhang, Cunqi Ye, Wei Xie, Jin Zhang, Haoxing Xu, Xudong Fu
During mouse preimplantation development, a subset of retrotransposons/genes are transiently expressed in the totipotent 2-cell (2C) embryos. These 2C transcripts rapidly shut down their expression beyond the 2C stage of embryos, promoting the embryo to exit from the 2C stage. However, the mechanisms regulating this shutdown remain unclear. Here, we identified that lysosomal catabolism played a role in the exit of the totipotent 2C state. Our results showed that the activation of embryonic lysosomal catabolism promoted the embryo to exit from the 2C stage and suppressed 2C transcript expression. Mechanistically, our results indicated that lysosomal catabolism suppressed 2C transcripts through replenishing cellular amino-acid levels, thereby inactivating transcriptional factors TFE3/TFEB and abolishing their transcriptional activation of 2C retrotransposons, MERVL (murine endogenous retrovirus-L)/MT2_Mm. Collectively, our study identified that lysosomal activity modulated the transcriptomic landscape and development in mouse embryos and identified an unanticipated layer of transcriptional control on early-embryonic retrotransposons from lysosomal signaling.
在小鼠胚胎植入前的发育过程中,一部分反转座子/基因在全能的 2 细胞(2C)胚胎中短暂表达。这些 2C 转录本在胚胎 2C 阶段之后迅速关闭表达,促使胚胎退出 2C 阶段。然而,这种关闭的调控机制仍不清楚。在这里,我们发现溶酶体分解代谢在退出全能 2C 状态中发挥了作用。我们的结果表明,胚胎溶酶体分解代谢的激活会促进胚胎退出 2C 阶段,并抑制 2C 转录物的表达。从机理上讲,我们的研究结果表明溶酶体分解代谢通过补充细胞氨基酸水平抑制了2C转录本,从而使转录因子TFE3/TFEB失活,取消了它们对2C逆转录病毒MERVL(鼠内源性逆转录病毒-L)/MT2_Mm的转录激活。总之,我们的研究发现溶酶体活动调节了小鼠胚胎的转录组格局和发育,并发现了溶酶体信号转导对早期胚胎逆转录转座子的转录控制的一个意想不到的层次。
{"title":"Lysosomal catabolic activity promotes the exit of murine totipotent 2-cell state by silencing early-embryonic retrotransposons","authors":"Hao Wu, Lanrui Cao, Xinpeng Wen, Jiawei Fan, Yuan Wang, Heyong Hu, Shuyan Ji, Yinli Zhang, Cunqi Ye, Wei Xie, Jin Zhang, Haoxing Xu, Xudong Fu","doi":"10.1016/j.devcel.2024.10.018","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.018","url":null,"abstract":"During mouse preimplantation development, a subset of retrotransposons/genes are transiently expressed in the totipotent 2-cell (2C) embryos. These 2C transcripts rapidly shut down their expression beyond the 2C stage of embryos, promoting the embryo to exit from the 2C stage. However, the mechanisms regulating this shutdown remain unclear. Here, we identified that lysosomal catabolism played a role in the exit of the totipotent 2C state. Our results showed that the activation of embryonic lysosomal catabolism promoted the embryo to exit from the 2C stage and suppressed 2C transcript expression. Mechanistically, our results indicated that lysosomal catabolism suppressed 2C transcripts through replenishing cellular amino-acid levels, thereby inactivating transcriptional factors TFE3/TFEB and abolishing their transcriptional activation of 2C retrotransposons, MERVL (murine endogenous retrovirus-L)/MT2_Mm. Collectively, our study identified that lysosomal activity modulated the transcriptomic landscape and development in mouse embryos and identified an unanticipated layer of transcriptional control on early-embryonic retrotransposons from lysosomal signaling.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"34 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of a non-canonical planar cell polarity pathway triggered by light in the developing mouse retina 在发育中的小鼠视网膜中发现由光引发的非经典平面细胞极性通路
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-18 DOI: 10.1016/j.devcel.2024.10.012
Michael Housset, Dominic Filion, Nelson Cortes, Hojatollah Vali, Craig A. Mandato, Christian Casanova, Michel Cayouette
The coordinated spatial arrangement of organelles within a tissue plane, known as planar cell polarity (PCP), is critical for organ development and function. Gradients of morphogens and their receptors typically set-up PCP, but whether non-molecular cues, akin to phototropism in plants, also play a part remains unknown. Here, we report that basal bodies of newborn photoreceptor cells in the mouse retina are positioned centrally on the apical surface but then move laterally during the first postnatal week, generating cell-intrinsic asymmetry in the retinal plane. After 1 week, when the eyes open, basal bodies of cone cilia, but not rods, become coordinated across the plane to face the center of the retina. We further show that light is essential for cone PCP, triggering a cascade in which cone transducin interacts with the G-protein-signaling modulator protein 2 (GPSM2) to establish PCP. This work identifies a non-canonical PCP pathway initiated by light.
细胞器在组织平面内的协调空间排列,即平面细胞极性(PCP),对于器官的发育和功能至关重要。形态诱导体及其受体的梯度通常会设置平面细胞极性,但非分子线索(类似于植物中的向光性)是否也在其中起作用仍是未知数。在这里,我们报告了小鼠视网膜中新生感光细胞的基底体在顶端表面居中定位,但在出生后第一周内向侧方移动,从而在视网膜平面上产生细胞内在不对称。1 周后,当眼睛睁开时,视锥纤毛的基底体(而不是视杆细胞)会协调地穿过视网膜平面,面向视网膜中心。我们进一步发现,光对视锥纤毛虫的协调性至关重要,它触发了一个级联,在这个级联中,视锥转导蛋白与 G 蛋白信号调节蛋白 2(GPSM2)相互作用,从而建立起协调性。这项研究发现了一条由光启动的非经典 PCP 通路。
{"title":"Identification of a non-canonical planar cell polarity pathway triggered by light in the developing mouse retina","authors":"Michael Housset, Dominic Filion, Nelson Cortes, Hojatollah Vali, Craig A. Mandato, Christian Casanova, Michel Cayouette","doi":"10.1016/j.devcel.2024.10.012","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.012","url":null,"abstract":"The coordinated spatial arrangement of organelles within a tissue plane, known as planar cell polarity (PCP), is critical for organ development and function. Gradients of morphogens and their receptors typically set-up PCP, but whether non-molecular cues, akin to phototropism in plants, also play a part remains unknown. Here, we report that basal bodies of newborn photoreceptor cells in the mouse retina are positioned centrally on the apical surface but then move laterally during the first postnatal week, generating cell-intrinsic asymmetry in the retinal plane. After 1 week, when the eyes open, basal bodies of cone cilia, but not rods, become coordinated across the plane to face the center of the retina. We further show that light is essential for cone PCP, triggering a cascade in which cone transducin interacts with the G-protein-signaling modulator protein 2 (GPSM2) to establish PCP. This work identifies a non-canonical PCP pathway initiated by light.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"99 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Initiation and maintenance of the pluripotent epiblast in pre-implantation human development is independent of NODAL signaling 植入前人类发育过程中多能上胚层的启动和维持与 NODAL 信号无关
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-18 DOI: 10.1016/j.devcel.2024.10.020
A. Sophie Brumm, Afshan McCarthy, Claudia Gerri, Todd Fallesen, Laura Woods, Riley McMahon, Athanasios Papathanasiou, Kay Elder, Phil Snell, Leila Christie, Patricia Garcia, Valerie Shaikly, Mohamed Taranissi, Paul Serhal, Rabi A. Odia, Mina Vasilic, Anna Osnato, Peter J. Rugg-Gunn, Ludovic Vallier, Caroline S. Hill, Kathy K. Niakan
The human blastocyst contains the pluripotent epiblast from which human embryonic stem cells (hESCs) can be derived. ACTIVIN/NODAL signaling maintains expression of the transcription factor NANOG and in vitro propagation of hESCs. It is unknown whether this reflects a functional requirement for epiblast development in human embryos. Here, we characterized NODAL signaling activity during pre-implantation human development. We showed that NANOG is an early molecular marker restricted to the nascent human pluripotent epiblast and was initiated prior to the onset of NODAL signaling. We further demonstrated that expression of pluripotency-associated transcription factors NANOG, SOX2, OCT4, and KLF17 were maintained in the epiblast in the absence of NODAL signaling activity. Genome-wide transcriptional analysis showed that NODAL signaling inhibition did not decrease NANOG transcription or impact the wider pluripotency-associated gene regulatory network. These data suggest differences in the signaling requirements regulating pluripotency in the pre-implantation human epiblast compared with existing hESC culture.
人类胚泡含有多能上胚层,人类胚胎干细胞(hESCs)可从上胚层中获得。ACTIVIN/NODAL信号维持转录因子NANOG的表达和hESCs的体外繁殖。目前还不清楚这是否反映了人类胚胎上胚层发育的功能要求。在这里,我们描述了人类胚胎植入前发育过程中 NODAL 信号活动的特征。我们发现,NANOG 是限制在新生人类多能上胚层的早期分子标记,并且在 NODAL 信号开始之前就已经启动。我们进一步证明,在没有 NODAL 信号活动的情况下,多能性相关转录因子 NANOG、SOX2、OCT4 和 KLF17 的表达在上胚层中得以维持。全基因组转录分析表明,NODAL 信号抑制不会降低 NANOG 的转录,也不会影响更广泛的多能性相关基因调控网络。这些数据表明,与现有的 hESC 培养相比,植入前人类上胚层中调节多能性的信号要求有所不同。
{"title":"Initiation and maintenance of the pluripotent epiblast in pre-implantation human development is independent of NODAL signaling","authors":"A. Sophie Brumm, Afshan McCarthy, Claudia Gerri, Todd Fallesen, Laura Woods, Riley McMahon, Athanasios Papathanasiou, Kay Elder, Phil Snell, Leila Christie, Patricia Garcia, Valerie Shaikly, Mohamed Taranissi, Paul Serhal, Rabi A. Odia, Mina Vasilic, Anna Osnato, Peter J. Rugg-Gunn, Ludovic Vallier, Caroline S. Hill, Kathy K. Niakan","doi":"10.1016/j.devcel.2024.10.020","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.020","url":null,"abstract":"The human blastocyst contains the pluripotent epiblast from which human embryonic stem cells (hESCs) can be derived. ACTIVIN/NODAL signaling maintains expression of the transcription factor <em>NANOG</em> and <em>in vitro</em> propagation of hESCs. It is unknown whether this reflects a functional requirement for epiblast development in human embryos. Here, we characterized NODAL signaling activity during pre-implantation human development. We showed that NANOG is an early molecular marker restricted to the nascent human pluripotent epiblast and was initiated prior to the onset of NODAL signaling. We further demonstrated that expression of pluripotency-associated transcription factors NANOG, SOX2, OCT4, and KLF17 were maintained in the epiblast in the absence of NODAL signaling activity. Genome-wide transcriptional analysis showed that NODAL signaling inhibition did not decrease <em>NANOG</em> transcription or impact the wider pluripotency-associated gene regulatory network. These data suggest differences in the signaling requirements regulating pluripotency in the pre-implantation human epiblast compared with existing hESC culture.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"76 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biased cell adhesion organizes the Drosophila visual motion integration circuit 偏向细胞粘附组织果蝇视觉运动整合回路
IF 11.8 1区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-15 DOI: 10.1016/j.devcel.2024.10.019
Yannick Carrier, Laura Quintana Rio, Nadia Formicola, Vicente de Sousa-Xavier, Maha Tabet, Yu-Chieh David Chen, Aicha Haji Ali, Maëva Wislez, Lisa Orts, Alexander Borst, Filipe Pinto-Teixeira
Layer-specific brain computations depend on neurons synapsing with specific partners in distinct laminae. In the Drosophila lobula plate, axons of the four subtypes of T4 and T5 visual motion direction-selective neurons segregate into four layers, where they synapse with distinct subsets of postsynaptic neurons. Here, we identify a layer-specific expression of different receptor-ligand pairs of the Beat and Side families of cell adhesion molecules between T4/T5s and their postsynaptic partners. Developmental genetic analysis demonstrate that Beat/Side-mediated interactions are required to restrict innervation of T4/T5 axons and the dendrites of their partners to a single layer. We show that Beat/Side interactions are not required for synaptogenesis. Instead, they contribute to synaptic specificity by biasing cellular adjacency, causing neurons to segregate in discrete layers, restricting partner availability before synaptogenesis. We propose that the emergence of synaptic specificity relies on a competitive dynamic among postsynaptic partners with shared Beat/Side expression to adhere with T4/T5s.
特定层的大脑计算依赖于神经元与不同层中特定伙伴的突触。在果蝇小叶板中,四种亚型的 T4 和 T5 视觉运动方向选择性神经元的轴突分离成四层,它们与不同的突触后神经元亚群发生突触。在这里,我们确定了在 T4/T5 与它们的突触后伙伴之间,细胞粘附分子 Beat 和 Side 家族的不同受体配体对的特定层表达。发育遗传分析表明,Beat/Side介导的相互作用是将T4/T5轴突及其伙伴树突的神经支配限制在单层所必需的。我们的研究表明,Beat/Side 相互作用并非突触发生所必需。相反,它们通过偏移细胞邻接性来促进突触特异性,使神经元分离在离散层中,在突触发生前限制伙伴的可用性。我们提出,突触特异性的出现依赖于具有共同 Beat/Side 表达的突触后伙伴之间的竞争动态,以粘附 T4/T5。
{"title":"Biased cell adhesion organizes the Drosophila visual motion integration circuit","authors":"Yannick Carrier, Laura Quintana Rio, Nadia Formicola, Vicente de Sousa-Xavier, Maha Tabet, Yu-Chieh David Chen, Aicha Haji Ali, Maëva Wislez, Lisa Orts, Alexander Borst, Filipe Pinto-Teixeira","doi":"10.1016/j.devcel.2024.10.019","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.019","url":null,"abstract":"Layer-specific brain computations depend on neurons synapsing with specific partners in distinct laminae. In the <em>Drosophila</em> lobula plate, axons of the four subtypes of T4 and T5 visual motion direction-selective neurons segregate into four layers, where they synapse with distinct subsets of postsynaptic neurons. Here, we identify a layer-specific expression of different receptor-ligand pairs of the Beat and Side families of cell adhesion molecules between T4/T5s and their postsynaptic partners. Developmental genetic analysis demonstrate that Beat/Side-mediated interactions are required to restrict innervation of T4/T5 axons and the dendrites of their partners to a single layer. We show that Beat/Side interactions are not required for synaptogenesis. Instead, they contribute to synaptic specificity by biasing cellular adjacency, causing neurons to segregate in discrete layers, restricting partner availability before synaptogenesis. We propose that the emergence of synaptic specificity relies on a competitive dynamic among postsynaptic partners with shared Beat/Side expression to adhere with T4/T5s.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"1 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Developmental cell
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1