Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-40-48
T. Solomay, E. I. Isayeva, E. N. Vetrova, A. I. Chernyshova, T. A. Semenenko, Lyudmila Grigoryevna Panteleyeva
The widespread spread of infection caused by the Epstein-Barr virus (EBV), the lack of registered vaccines, the possibility of implementing a transmission mechanism in medical organizations, determine disinfection as the main method of preventing this disease. The aim is to evaluate the effectiveness of chemical disinfection agents approved for use in the Russian Federation in relation to EBV-infected cell culture with quantitative DNA determination by polymerase chain reaction. Working solutions of complex disinfectants used for the treatment of medical devices were introduced into samples of EBV-infected B-cell culture in the concentration and exposure specified in the instructions (0.1 %; 60 min.). Then, the quantitative determination of the EBV DNA was performed. Chlorine-containing disinfectants, combined preparations based on aldehydes and guanidines per hour, as well as guanidines per hour and triamines, provided complete destruction of genetic material, while the combination of H with triamines led only to a decrease in the concentration of EBV DNA from 5.0 · 106 copies/ml to 2.4 · 106 copies/ml (by 2.1 times), which may not be enough to fully ensure biological safety when processing medical devices (sensors of ultrasound diagnostics devices, etc.), which are not subject to sterilization. The inability to determine the viability of EBV after treatment with disinfectants due to the initial presence of the virus in the B-cell culture makes the molecular biological method the only acceptable one at this stage and requires the search for new models – cell cultures intact to EBV or biological objects (laboratory animals). Keywords: Epstein-Barr virus, disinfection, quaternary ammonium compounds, triamines, aldehydes, guanidines, active chlorine releasing agents.
{"title":"Evaluation of the effectiveness of chemical disinfection agents against the Epstein-Barr virus","authors":"T. Solomay, E. I. Isayeva, E. N. Vetrova, A. I. Chernyshova, T. A. Semenenko, Lyudmila Grigoryevna Panteleyeva","doi":"10.35411/2076-457x-2021-3-40-48","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-40-48","url":null,"abstract":"The widespread spread of infection caused by the Epstein-Barr virus (EBV), the lack of registered vaccines, the possibility of implementing a transmission mechanism in medical organizations, determine disinfection as the main method of preventing this disease. The aim is to evaluate the effectiveness of chemical disinfection agents approved for use in the Russian Federation in relation to EBV-infected cell culture with quantitative DNA determination by polymerase chain reaction. Working solutions of complex disinfectants used for the treatment of medical devices were introduced into samples of EBV-infected B-cell culture in the concentration and exposure specified in the instructions (0.1 %; 60 min.). Then, the quantitative determination of the EBV DNA was performed. Chlorine-containing disinfectants, combined preparations based on aldehydes and guanidines per hour, as well as guanidines per hour and triamines, provided complete destruction of genetic material, while the combination of H with triamines led only to a decrease in the concentration of EBV DNA from 5.0 · 106 copies/ml to 2.4 · 106 copies/ml (by 2.1 times), which may not be enough to fully ensure biological safety when processing medical devices (sensors of ultrasound diagnostics devices, etc.), which are not subject to sterilization. The inability to determine the viability of EBV after treatment with disinfectants due to the initial presence of the virus in the B-cell culture makes the molecular biological method the only acceptable one at this stage and requires the search for new models – cell cultures intact to EBV or biological objects (laboratory animals). Keywords: Epstein-Barr virus, disinfection, quaternary ammonium compounds, triamines, aldehydes, guanidines, active chlorine releasing agents.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81978166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-49-54
T. A. Davlianidze, O. Eremina, V. V. Olifer
The effectiveness of insecticidal baits based on chlorfenapyr and hydramethylnone against colonies of the Pharaoh ant has been studied. Sugar baits containing 0.1–1.0 % chlorfenapyr are ineffective because after 4 weeks of the experiment, the viability of the colonies was not changed. Sugar baits based on 1 % hydramethylnon effectively destroyed workers, the effect on queens was weak, and depletion of the colony was achieved. Commercially produced baits effectively and completely destroy ant colonies both in the absence and in the presence of alternative food. Keywords: Pharaoh ant, baits, chlorfenapyr, hydramethylnon.
{"title":"Efficacy of chlorfenapyr- and hydramethylnon-based baits on laboratory colonies of the pharaoh ant","authors":"T. A. Davlianidze, O. Eremina, V. V. Olifer","doi":"10.35411/2076-457x-2021-3-49-54","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-49-54","url":null,"abstract":"The effectiveness of insecticidal baits based on chlorfenapyr and hydramethylnone against colonies of the Pharaoh ant has been studied. Sugar baits containing 0.1–1.0 % chlorfenapyr are ineffective because after 4 weeks of the experiment, the viability of the colonies was not changed. Sugar baits based on 1 % hydramethylnon effectively destroyed workers, the effect on queens was weak, and depletion of the colony was achieved. Commercially produced baits effectively and completely destroy ant colonies both in the absence and in the presence of alternative food. Keywords: Pharaoh ant, baits, chlorfenapyr, hydramethylnon.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88735858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-16-28
G. Frolov, N. V. Zavyalova, I. Lundovskikh, M. R. Shabalina, I. P. Pogorelskiy, K. I. Gurin, A. V. Mironin
The results are presented of a comparative study of the sporicidal activity of alcohol sols of nanoparticles of titanium, copper, zinc and tantalum metals in the course of their interaction with a rehydrated lyophilized culture of spores of the vaccine strain Bacillus anthracis STI-1 with the loss of the ability of spores to germinate in nutrient media under optimal conditions using bacteriology and electronic microscopy. The most pronounced sporicidal effect is observed when titanium alcohol sol is exposed to spores. At the same time, spores of B. anthracis STI-1 in titanium alcohol sol are affected by ethyl alcohol, which causes dehydration and denaturation of the protein, the antiseptic cetylpyridinium chloride as a surfactant, which is also a strong electrophilic agent that destroys the exosporium and spore shells, and titanium nanoparticles, which, due to the high surface energy when exposed to spores, cause their pronounced massive adhesion. In addition, titanium nanoparticles, the zeta (? -) value of the potential of which is -44.5 mV, cause a stable energy state of the dispersed system of alcohol sol, which acts as a biocatalyst for intracellular enzymes, causing the hydrolysis of polymer structures of the spore shells and cortex, as well as destroyed nucleoid – an area of spores containing structured genetic material. Spores that do not have a nucleoid in electron microscopy acquire the form of "shadows". As a result of the action of titanium alcohol sol, the activation of germination and the formation of the so-called "metabolic" spores, from which the vegetative cells of B. anthracis STI-1 should have been formed, are prevented. The reason for the high sporicidal efficiency of the alcohol sol of titanium nanoparticles is associated both with the structure and properties and with the technology of synthesis of titanium nanoparticles in the low-temperature plasma zone with the formation, due to the excess of the surfactant cetylpyridinium chloride, of a two-layer shell around the metal nanoparticles, which leads to their "conservation" until the moment of contact with spores and a sharp slowdown in their oxidation in the aquatic environment. The destruction of the formed two-layer shell of metal nanoparticles begins with contact with spores with the simultaneous onset of the formation of titanium ions from the phase of nanoparticles, which actively destroy chemically the shells of the spores. Keywords: microorganisms, Bacillus anthracis STI-1, spores, metal nanoparticles, zeta potential, disinfectant compositions, sporicidal activity.
{"title":"Possible mechanisms of the destruction of spores of the vaccine strain Bacillus anthracis STI-1 under the influence of alcohol sols of metal nanoparticles","authors":"G. Frolov, N. V. Zavyalova, I. Lundovskikh, M. R. Shabalina, I. P. Pogorelskiy, K. I. Gurin, A. V. Mironin","doi":"10.35411/2076-457x-2021-3-16-28","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-16-28","url":null,"abstract":"The results are presented of a comparative study of the sporicidal activity of alcohol sols of nanoparticles of titanium, copper, zinc and tantalum metals in the course of their interaction with a rehydrated lyophilized culture of spores of the vaccine strain Bacillus anthracis STI-1 with the loss of the ability of spores to germinate in nutrient media under optimal conditions using bacteriology and electronic microscopy. The most pronounced sporicidal effect is observed when titanium alcohol sol is exposed to spores. At the same time, spores of B. anthracis STI-1 in titanium alcohol sol are affected by ethyl alcohol, which causes dehydration and denaturation of the protein, the antiseptic cetylpyridinium chloride as a surfactant, which is also a strong electrophilic agent that destroys the exosporium and spore shells, and titanium nanoparticles, which, due to the high surface energy when exposed to spores, cause their pronounced massive adhesion. In addition, titanium nanoparticles, the zeta (? -) value of the potential of which is -44.5 mV, cause a stable energy state of the dispersed system of alcohol sol, which acts as a biocatalyst for intracellular enzymes, causing the hydrolysis of polymer structures of the spore shells and cortex, as well as destroyed nucleoid – an area of spores containing structured genetic material. Spores that do not have a nucleoid in electron microscopy acquire the form of \"shadows\". As a result of the action of titanium alcohol sol, the activation of germination and the formation of the so-called \"metabolic\" spores, from which the vegetative cells of B. anthracis STI-1 should have been formed, are prevented. The reason for the high sporicidal efficiency of the alcohol sol of titanium nanoparticles is associated both with the structure and properties and with the technology of synthesis of titanium nanoparticles in the low-temperature plasma zone with the formation, due to the excess of the surfactant cetylpyridinium chloride, of a two-layer shell around the metal nanoparticles, which leads to their \"conservation\" until the moment of contact with spores and a sharp slowdown in their oxidation in the aquatic environment. The destruction of the formed two-layer shell of metal nanoparticles begins with contact with spores with the simultaneous onset of the formation of titanium ions from the phase of nanoparticles, which actively destroy chemically the shells of the spores. Keywords: microorganisms, Bacillus anthracis STI-1, spores, metal nanoparticles, zeta potential, disinfectant compositions, sporicidal activity.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90713688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-55-62
T. A. Davlianidze, O. Eremina, V. V. Olifer
The insecticidal properties of proinsecticides fipronil, indoxacarb, and methomyl were studied against the colonies of Pharaoh ant. It was found that sugar food baits based on fipronil or indoxacarb are effective against colonies of Pharaoh ant in the concentration ranges 0.001–0.1 % and 0.1–1.0 %, respectively. Baits based on 1 % methomyl are also highly effective. In the presence of alternative food, the mortality of workers and queens was reduced. The food attractiveness of ant baits should be increased by introducing honey, peanut butter, and other attractants. Keywords: proinsecticides, fipronil, indoxacarb, methomyl, baits, Pharaoh ant.
{"title":"Study of the efficiency of baits based on proinsecticides in relation to susceptible laboratory culture of pharaoh ants","authors":"T. A. Davlianidze, O. Eremina, V. V. Olifer","doi":"10.35411/2076-457x-2021-3-55-62","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-55-62","url":null,"abstract":"The insecticidal properties of proinsecticides fipronil, indoxacarb, and methomyl were studied against the colonies of Pharaoh ant. It was found that sugar food baits based on fipronil or indoxacarb are effective against colonies of Pharaoh ant in the concentration ranges 0.001–0.1 % and 0.1–1.0 %, respectively. Baits based on 1 % methomyl are also highly effective. In the presence of alternative food, the mortality of workers and queens was reduced. The food attractiveness of ant baits should be increased by introducing honey, peanut butter, and other attractants. Keywords: proinsecticides, fipronil, indoxacarb, methomyl, baits, Pharaoh ant.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85276872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-28-35
L. Panteleeva
More than 40 different species of the family Coronaviridae are known. The greatest epidemiological significance was acquired by SARS-CoV-2, the causative agent of a new coronavirus infection-COVID-19. It can persist on the surfaces of various objects from several hours to several days. It is sensitive to disinfectants from different chemical groups: chlorine-, oxygen-active, cationic surfactants, alcohols, aldehydes, and iodine-containing agents. Keywords: coronaviruses, survival rate, disinfectants, virucidal activity, virus sensitivity.
{"title":"Sensitivity of coronaviruses to disinfectants according to the results of foreign and domestic studies","authors":"L. Panteleeva","doi":"10.35411/2076-457x-2021-3-28-35","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-28-35","url":null,"abstract":"More than 40 different species of the family Coronaviridae are known. The greatest epidemiological significance was acquired by SARS-CoV-2, the causative agent of a new coronavirus infection-COVID-19. It can persist on the surfaces of various objects from several hours to several days. It is sensitive to disinfectants from different chemical groups: chlorine-, oxygen-active, cationic surfactants, alcohols, aldehydes, and iodine-containing agents. Keywords: coronaviruses, survival rate, disinfectants, virucidal activity, virus sensitivity.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82326949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-69-73
Tatyana Ivanovna Avdyukhina, T. A. Semenova, Andrey Anatolievich Androsov, T. V. Gololobova, T. Suranova
The article highlights in detail the features of the periodic accreditation of specialists in 2021, gives detailed recommendations to doctors on the formation of an educational trajectory and on the preparation of documents for accreditation. The authors present the rules of operation of the Federal Accreditation Center, listed regulatory legal documents. Keywords: primary accreditation of specialists, professional development programs, portfolio, Department of Parasitology and Disinfection.
{"title":"Features of periodic accreditation of specialists in the specialties «Disinfection», «Parasitology» and other specialties in 2021","authors":"Tatyana Ivanovna Avdyukhina, T. A. Semenova, Andrey Anatolievich Androsov, T. V. Gololobova, T. Suranova","doi":"10.35411/2076-457x-2021-3-69-73","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-69-73","url":null,"abstract":"The article highlights in detail the features of the periodic accreditation of specialists in 2021, gives detailed recommendations to doctors on the formation of an educational trajectory and on the preparation of documents for accreditation. The authors present the rules of operation of the Federal Accreditation Center, listed regulatory legal documents. Keywords: primary accreditation of specialists, professional development programs, portfolio, Department of Parasitology and Disinfection.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72942331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-63-68
O. Eremina, V. V. Olifer
When eating poisoned baits based on 0.1–1.0 % pyriproxyfen, within 6 weeks killed up to 50 % of German cockroaches of the S-NIID strain, and 12 % of cockroaches of the OBN strain. The resulting oothecae turned out to be defective and the second generation of cockroaches did not hatch. Morphological changes consisted were in the deformation of the wings (rudimentary, curved, twisted) and in the inability of the larvae to transform into adults. In experiments with an alternative food, similar results were obtained, females formed more oothecae, but the emergence of new generation larvae was not recorded. The consumption of pyriproxyfen-based baits by Pharaoh ant led to the elimination of colonies. A significant decrease in brood number was recorded at 3–6 weeks, and complete death of brood was observed at 6–8 weeks in all treated colonies. The absence of brood can be explained by both mortality as a result of developmental disorders and the cessation of egg laying by queens. Keywords: insect growth regulators, German cockroach, Pharaoh ant, pyriproxifen, hydroprene.
{"title":"Effect of insect growth regulators on two german cockroach strains and pharaoh ants","authors":"O. Eremina, V. V. Olifer","doi":"10.35411/2076-457x-2021-3-63-68","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-63-68","url":null,"abstract":"When eating poisoned baits based on 0.1–1.0 % pyriproxyfen, within 6 weeks killed up to 50 % of German cockroaches of the S-NIID strain, and 12 % of cockroaches of the OBN strain. The resulting oothecae turned out to be defective and the second generation of cockroaches did not hatch. Morphological changes consisted were in the deformation of the wings (rudimentary, curved, twisted) and in the inability of the larvae to transform into adults. In experiments with an alternative food, similar results were obtained, females formed more oothecae, but the emergence of new generation larvae was not recorded. The consumption of pyriproxyfen-based baits by Pharaoh ant led to the elimination of colonies. A significant decrease in brood number was recorded at 3–6 weeks, and complete death of brood was observed at 6–8 weeks in all treated colonies. The absence of brood can be explained by both mortality as a result of developmental disorders and the cessation of egg laying by queens. Keywords: insect growth regulators, German cockroach, Pharaoh ant, pyriproxifen, hydroprene.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82567641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.35411/2076-457x-2021-3-5-15
I. Krylova, R. R. Zhdanov, Chetina Oksana Aleksandrovna
The article presents the results of a qualitative and quantitative analysis of the microbiome of places where dogs of service breeds are kept, obtained under the conditions of rotation of medicines for an active substance. In the period from 2017 to 2021, dynamic observations were made to assess the effectiveness of preventive disinfection measures carried out in the cynological town. The object of the study was the micro-and mycoflora of more than 20 dog enclosures in two versions: before disinfection and after mechanical treatment and fine-drip irrigation with preparations recommended for disinfection of veterinary surveillance facilities. Using differential diagnostic nutrient media, representatives of different groups of microorganisms were isolated from the surfaces of inventory, poilushki, the floor and walls of the walking enclosures. A qualitative analysis of biodiversity revealed that microbial isolates are conditionally pathogenic microorganisms and can be potentially dangerous infectious agents. The results showed that disinfection measures were not sufficiently effective against the selected groups of microorganisms. Using elective nutrient media, cultures of yeast-like and mold-like micromycetes were isolated from the surfaces of walking enclosures. The analysis of the data showed that both decompositions, claimed to be effective against mold micromycetes, do not withstand the contaminating load. The method of small-drop irrigation used for processing the walking of the kennels of the cynological town turned out to be low-effective in relation to the isolated microbial and fungal isolates. Within the framework of compliance with the sanitary and hygienic regime, an algorithm for organizing preventive disinfection measures in the cynological unit has been developed, based on the well-coordinated interaction of support services: medical, logistics, sanitary and veterinary. The research conducted jointly with representatives of the veterinary and sanitary service, with cadets of the cynological faculty, is of great practical importance for the formation of the professional competence of future officers – specialists-cynologists, heads of cynological units. The developed recommendations make it possible to adjust the algorithm of sanitary and hygienic measures in the cynological town and are of great importance in solving health-saving issues and maintaining the number of dogs of service breeds. Keywords: preventive disinfection; sanitary-indicative microorganisms; micromycetes; health care of dogs; cynologists.
{"title":"Evaluation of the effectiveness of preventive disinfection measures based on a comprehensive analysis of the microbiome of service dogs‘ places of detentionКоррозионное действие растворов N,N-бис(3-аминопропил)додециламина и их смесей с алкилдиметилбензиламмоний хлоридом","authors":"I. Krylova, R. R. Zhdanov, Chetina Oksana Aleksandrovna","doi":"10.35411/2076-457x-2021-3-5-15","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-3-5-15","url":null,"abstract":"The article presents the results of a qualitative and quantitative analysis of the microbiome of places where dogs of service breeds are kept, obtained under the conditions of rotation of medicines for an active substance. In the period from 2017 to 2021, dynamic observations were made to assess the effectiveness of preventive disinfection measures carried out in the cynological town. The object of the study was the micro-and mycoflora of more than 20 dog enclosures in two versions: before disinfection and after mechanical treatment and fine-drip irrigation with preparations recommended for disinfection of veterinary surveillance facilities. Using differential diagnostic nutrient media, representatives of different groups of microorganisms were isolated from the surfaces of inventory, poilushki, the floor and walls of the walking enclosures. A qualitative analysis of biodiversity revealed that microbial isolates are conditionally pathogenic microorganisms and can be potentially dangerous infectious agents. The results showed that disinfection measures were not sufficiently effective against the selected groups of microorganisms. Using elective nutrient media, cultures of yeast-like and mold-like micromycetes were isolated from the surfaces of walking enclosures. The analysis of the data showed that both decompositions, claimed to be effective against mold micromycetes, do not withstand the contaminating load. The method of small-drop irrigation used for processing the walking of the kennels of the cynological town turned out to be low-effective in relation to the isolated microbial and fungal isolates. Within the framework of compliance with the sanitary and hygienic regime, an algorithm for organizing preventive disinfection measures in the cynological unit has been developed, based on the well-coordinated interaction of support services: medical, logistics, sanitary and veterinary. The research conducted jointly with representatives of the veterinary and sanitary service, with cadets of the cynological faculty, is of great practical importance for the formation of the professional competence of future officers – specialists-cynologists, heads of cynological units. The developed recommendations make it possible to adjust the algorithm of sanitary and hygienic measures in the cynological town and are of great importance in solving health-saving issues and maintaining the number of dogs of service breeds. Keywords: preventive disinfection; sanitary-indicative microorganisms; micromycetes; health care of dogs; cynologists.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79671078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-01DOI: 10.35411/2076-457x-2021-2-44-50
P. A. Mochalkin, A. P. Mochalkin, E. G. Stepanov, L. A. Farvazova, N. Popov, Svetlana Nikolaevna Nemtsova
It was established that 17008 HFRS cases were reported in 53 (out of 54) administrative districts and 12 cities (out of 14) of the Republic of Bashkortostan in 2009-2018. The most intense epidemiological situation (a long-term incidence rate of 48,5 per 100 thousand of the population) was observed within the boundaries of the forest-steppe landscape zone, where. more than 86 % of all cases of infection are recorded of the Republic of Bashkortostan in 2009-2018. The share of infections among the urban population was 64.0 %, the rural one – 36.0 %. In the long-term aspect, according to the conditions of HFRS acquisition, short-term visits to the forest area prevailed – up to 80 %, in household settings – up to 14 % of all cases of the disease. Seasonal increases in the incidence of HFRS take place in May-June – up to 15 % and in September-December – over 46 % of the annual incidence. The presence of synchronicity in the dynamics of HFRS morbidity in rural and urban populations within the boundaries of the administrative district was detected. It has been substantiated that in order to reduce the level of HFRS morbidity among the rural and urban population, it is necessary to provide for an increase in the volume and frequency of preventive (anti-epidemic) measures in the vicinity of rural settlements, regional centers, urban-type settlements and large cities located near forests, within the boundaries of the entire area of the administrative district.
{"title":"Features of the dynamics of hemorrhagic fever with renal syndrome incidence in the Republic of Bashkortostan","authors":"P. A. Mochalkin, A. P. Mochalkin, E. G. Stepanov, L. A. Farvazova, N. Popov, Svetlana Nikolaevna Nemtsova","doi":"10.35411/2076-457x-2021-2-44-50","DOIUrl":"https://doi.org/10.35411/2076-457x-2021-2-44-50","url":null,"abstract":"It was established that 17008 HFRS cases were reported in 53 (out of 54) administrative districts and 12 cities (out of 14) of the Republic of Bashkortostan in 2009-2018. The most intense epidemiological situation (a long-term incidence rate of 48,5 per 100 thousand of the population) was observed within the boundaries of the forest-steppe landscape zone, where. more than 86 % of all cases of infection are recorded of the Republic of Bashkortostan in 2009-2018. The share of infections among the urban population was 64.0 %, the rural one – 36.0 %. In the long-term aspect, according to the conditions of HFRS acquisition, short-term visits to the forest area prevailed – up to 80 %, in household settings – up to 14 % of all cases of the disease. Seasonal increases in the incidence of HFRS take place in May-June – up to 15 % and in September-December – over 46 % of the annual incidence. The presence of synchronicity in the dynamics of HFRS morbidity in rural and urban populations within the boundaries of the administrative district was detected. It has been substantiated that in order to reduce the level of HFRS morbidity among the rural and urban population, it is necessary to provide for an increase in the volume and frequency of preventive (anti-epidemic) measures in the vicinity of rural settlements, regional centers, urban-type settlements and large cities located near forests, within the boundaries of the entire area of the administrative district.","PeriodicalId":11317,"journal":{"name":"Disinfection affairs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80504970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-01DOI: 10.35411/2076-457x-2021-2-22-29
O. M. Germant, Natalya Igorevna Shashina
With the aim of unifying and simplifying the assessment of categories of the effectiveness of the repellents funds audited 473 studies 245 samples of repellent remedies on the basis of DEET in various formulations, made in 1995–2019 employees of the Scientific Research Disinfесtology Institute in laboratory and in field conditions. The experiments were conducted in parallel with the standards of the corresponding or expected categories of effectiveness of repellent remedies. The concordance of the results of standard laboratory experiments on a model object Ae. aegypti mosquitoes and the results obtained from testing repellent agents against natural populations of flying blood-sucking insects and taiga ticks is proved. The effectiveness of repellent remedies based on other repellent substances, as well as the effectiveness against other species, should continue to be investigated in full-scale conditions for individuals of natural populations. These remedies should be included in the system of categories of effectiveness of repellent products.
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