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Prospects for use of entomopathogenic fungi for the biocontrol of ixodid ticks (Ixodida: Ixodidae) 昆虫病原真菌在蜱虫生物防治中的应用前景(蜱虫目:蜱虫科)
Pub Date : 2023-06-01 DOI: 10.35411/2076-457x-2023-2-44-53
K. Krivonos, O. Eremina, V. V. Olifer
Data from 47 sources of scientific literature on the prospects for the use of biopesticides based on Metarhizium anisopliae, Beauveria bassiana and others species of entomopathogenic fungi to reduce the number of ixodid ticks Ixodes ricinus, I. scapularis, Dermacentor variabilis, D. reticulatus and Rhipicephalus sanguineus are presented. A review was made of data on the effectiveness of mycoacaricides used abroad against ixodid ticks, leading to a reduction in the number of ticks by 87–96?% after 3 weeks and by 53–74?% after 5 weeks after treatment. The use of the M. anisopliae F52 strain can become another tool for an integrated approach to the control of ticks in the residential landscape. An integrated method for controlling the number of ixodid ticks is presented, including the use of the entomopathogenic fungus M. anisopliae, the treatment of areas with acaricides, and the feeding of systemic insecticidal baits based on fipronil to rodents, which eliminate the larvae and nymphs of the tick when fed on a host. The experience of limited trials of preparative forms of mycoinsecticides that did not show high efficiency against the taiga tick Ixodes persulcatus in our country is described. Keywords: ixodid ticks, entomopathogenic fungi, Metarhizium, Beauveria, biopesticides.
从47篇文献资料中,综述了以金龟子绿僵菌、球孢白僵菌等昆虫病原真菌为基础的生物农药在减少蜱虫数量方面的应用前景,包括蓖麻蜱、镰形蜱、变皮蜱、网状蜱和血根蜱。对国外使用的杀真菌剂对伊蚊蜱的有效性数据进行了审查,导致蜱的数量减少了87-96 ?3周后和53-74岁?%,治疗后5周。利用绿僵菌F52菌株可以成为综合控制住宅景观中蜱虫的另一种工具。本文提出了一种控制蜱虫数量的综合方法,包括利用昆虫病原真菌金龟子菌,用杀螨剂处理区域,以及给啮齿动物喂食以氟虫腈为基础的全身杀虫诱饵,以消灭蜱虫的幼虫和若虫。本文叙述了在我国对针叶林蜱(taiiga tick persulcatus)没有显示出高效率的制备型真菌杀虫剂进行有限试验的经验。关键词:蜱虫,昆虫病原真菌,绿僵菌,白僵菌,生物农药
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引用次数: 0
Study on the efficacy of fipronil-based food baits for multiresistant german cockroach 氟虫腈食物饵对多重抗性德国蜚蠊的药效研究
Pub Date : 2023-06-01 DOI: 10.35411/2076-457x-2023-2-37-44
O. Eremina, V. V. Olifer, K. Krivonos
Insecticide food baits based on fipronil are widely represented in Russia. The effectiveness of six food baits containing fipronil and its mixtures with other active ingredients was evaluated against four laboratory strains of the German cockroach – insecticide-susceptible S-NIID and multi-resistant MAG, CR, and DM. A slowdown in the manifestation of symptoms of poisoning in cockroaches of resistant strains was revealed. The resistance indices calculated by LT50 and LT95 values were high: 5–12? for males and 3–13? for females, and 10–>21? for males and >14? for females, respectively. By day 21, 10?% of the DM males and 15–85?% of the females of all three resistant strains survived. Keywords: fipronil, German cockroach, resistance, food baits, phenylpyrazoles.
以氟虫腈为基础的杀虫剂食物诱饵在俄罗斯广泛使用。用含氟虫腈及其与其他有效成分混合的6种饵料,对4种实验室德国蜚蠊(对杀虫剂敏感的S-NIID和多重耐药的MAG、CR和DM)的中毒效果进行了评价。结果表明,耐药菌株的中毒症状表现有所减缓。由LT50和LT95值计算的抗性指数较高:5-12 ?男性和3-13岁?对于女性,10 - >21?男性和>14?分别为女性。第21天,第10天?%的DM男性和15-85 ?所有三种耐药菌株的雌性都存活了。关键词:氟虫腈,德国蜚蠊,抗药性,食物诱饵,苯吡唑。
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引用次数: 0
Use of automatic steam sterilizer for disinfection of immunobiological waste contaminated with pathogenic biological agents 应用自动蒸汽灭菌器对受致病性生物制剂污染的免疫生物废物进行消毒
Pub Date : 2023-06-01 DOI: 10.35411/2076-457x-2023-2-14-22
A. D. Belousov, M. V. Antonycheva, O. A. Volokh, Elena Gennadievna Abramova, Marina Vyacheslavovna Gordeeva, Mikhail Mikhailovich Chalbushev, A. K. Nikiforov
One of the ways to neutralize immunobiological production waste is their treatment in sterilizers with saturated water vapor under pressure. Currently, medical and pharmaceutical industries and laboratories use sterilizers with sterilization and disinfection programs preset by manufacturers. Decontamination modes are defined in Federal sanitary rules, norms and hygienic standards, SanPiN 3.3686-21, while the parameters of preliminary phases of the cycle must be tested experimentally and validated for specific conditions. Within the frames of this study, we have tested the modes of disinfection of an automatic steam sterilizer by the example of waste products from the production of cholera vaccine and anti-rabies immunoglobulin manufactured by the FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor. The wastes are used nutrient media, carcasses of laboratory animals (rabbits) and consumables. The chamber was loaded with «clean» objects in quantity and configuration, repeating the loading of material with pathogenic biological agents (PBA). The mode of inactivation inside the chamber and the objects was controlled by physical, chemical and biological methods. Based on the results of the experiments, corrective actions were taken to change the program and design of containers for autoclaving. It was concluded that the default disinfection programs set by manufacturers do not fully take into account specific conditions and need to be adjusted with further validation. It is necessary to develop standard operating procedures for personnel involved in the decontamination of pathogenic materials, in which should be stated the order of placement of objects and the means of control in the containers with PBA and the sterilizer chamber. Keywords: steam sterilizers, pathogenic biological agents, disinfection with saturated water vapor, phases of the steam disinfection cycle, validation.
其中一种中和免疫生物生产废物的方法是在高压下用饱和水蒸气灭菌器处理它们。目前,医疗和制药行业和实验室使用的消毒器是由制造商预置的灭菌和消毒程序。去污模式在联邦卫生规则、规范和卫生标准SanPiN 3.3686-21中有定义,而循环的初步阶段的参数必须经过实验测试并针对特定条件进行验证。在本研究的框架内,我们以FSSI俄罗斯Rospotrebnadzor的“微生物”抗鼠疫研究所生产的霍乱疫苗和抗狂犬病免疫球蛋白的废料为例,测试了自动蒸汽灭菌器的消毒模式。这些废物是用过的营养培养基、实验动物(兔子)的尸体和消耗品。该室在数量和结构上装载“清洁”物体,重复装载病原体生物制剂(PBA)的材料。通过物理、化学和生物三种方法控制实验室内和实验对象的灭活方式。根据实验结果,对高压灭菌容器的程序和设计进行了改进。结论是,生产企业设置的默认消毒方案没有充分考虑具体情况,需要进一步验证进行调整。有必要为参与病原材料净化的人员制定标准操作程序,其中应说明物品的放置顺序和在装有PBA的容器和灭菌室中的控制方法。关键词:蒸汽灭菌器,致病性生物制剂,饱和水蒸气消毒,蒸汽消毒循环阶段,验证
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引用次数: 0
Evaluation of nanoparticles as promising alternative antimicrobials 纳米颗粒作为有前途的替代抗菌剂的评价
Pub Date : 2023-06-01 DOI: 10.35411/2076-457x-2023-2-5-14
O. Zherebyateva, L. Aznabaeva, Diana Galimovna Ukubaeva, E. A. Mikhailova, Alexandr S. Pankov, O. Bolshakova, S. Sarantseva, V. Lebedev, Andrey Yuryevich Mironov
The search for new substances capable of inhibiting the growth of microorganisms is relevant. Therefore, an assessment was made of the effect of carbon nanoparticles on the growth and biofilm formation of microbial isolates from the surfaces of anthropogenic environmental objects. It has been established that carbon nanoparticles on the C60 + PVP + AC960 diamond platform are able to suppress the growth, reproduction and ability to biofilm formation of gram-positive and gram-negative prokaryotes, as well as fungi of the genus Candida and Aspergillus. Under the influence of C60+AC960 fullerenes, the biofilm-generation ability of Staphylococcus epidermidis, Bacillus cereus, Escherichia coli, and Candida albicans was significantly inhibited. These data provide a basis for further study of these compounds as a promising antimicrobial agent. Keywords: Antimicrobial action, carbon nanoparticles, biofilm formation, fullerenes, nanodiamonds.
寻找能够抑制微生物生长的新物质是相关的。因此,本文评价了碳纳米颗粒对人为环境物体表面微生物分离株生长和生物膜形成的影响。已经证实,C60 + PVP + AC960钻石平台上的碳纳米颗粒能够抑制革兰氏阳性和革兰氏阴性原核生物以及假丝酵母属真菌和曲霉属真菌的生长、繁殖和生物膜形成能力。在C60+AC960富勒烯的影响下,表皮葡萄球菌、蜡样芽孢杆菌、大肠杆菌和白色念珠菌的生物膜生成能力被显著抑制。这些数据为进一步研究这些化合物作为一种有前景的抗菌剂提供了基础。关键词:抗菌作用,纳米碳,生物膜形成,富勒烯,纳米金刚石。
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引用次数: 0
Direct and combined effects of ozone on biofilm Pseudomonas aeruginosa 臭氧对铜绿假单胞菌生物膜的直接及联合影响
Pub Date : 2023-03-01 DOI: 10.35411/2076-457x-2023-1-30-40
Anastasiya Alekseevna Ushakova, I. Karimov, S. D. Borisov, A. S. Pankov, A. G. Korneev
In modern medicine, the issue of combating biofilms, including various surfaces in medical facilities, is acute. Ozonation is an environmentally friendly and effective technology in the fight against microbial pollution, including biofilms. The article presents studies of the disinfecting properties of ozone and its combination with various disinfectants on planktonic and adhered (in the biofilm form) Pseudomonas aeruginosa cells. As a result, it was found that ozone is highly effective against various forms of bacterial life, and also requires longer exposure in the presence of organic pollution. When combined with hydrogen peroxide, it shows the greatest bactericidal effect not only on the destruction of the biofilm, but also on the viability of the cells themselves. Keywords: biofilm, ozone disinfection, destruction of biofilm.
在现代医学中,对抗生物膜的问题,包括医疗设施中的各种表面,是一个紧迫的问题。臭氧化是一种环境友好且有效的对抗微生物污染的技术,包括生物膜。本文研究了臭氧对浮游和粘附(以生物膜形式)的铜绿假单胞菌细胞的消毒性能及其与各种消毒剂的联合作用。因此,人们发现臭氧对各种形式的细菌生命都非常有效,而且在有机污染的情况下也需要更长时间的接触。当与过氧化氢结合时,它显示出最大的杀菌效果,不仅对生物膜的破坏,而且对细胞本身的生存能力。关键词:生物膜,臭氧消毒,生物膜破坏。
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引用次数: 0
Synthesis and study of a disinfectant based on diethanolamine 二乙醇胺类消毒剂的合成与研究
Pub Date : 2023-03-01 DOI: 10.35411/2076-457x-2023-1-56-62
V. Farzaliev, M. Abbasova, Oruc Garib Nabiev, N. A. Alieva, L. Safarova, G. M. Kulieva
The interaction of diethanolamine with formaldehyde at a ratio of initial reagents of 2?:?3, respectively, obtained a preparation that is an equimolar mixture of 3-(?-hydroxyethyl)-1,3-oxazolidine and its methylol derivative – 3-(?-hydroxyoxymethyl-ethyl)-1,3-oxazolidine. The paper presents the results of a study of the antimicrobial activity of the preparation against microorganisms – S. aureus, E. coli, as the most resistant species of bacteria that cause nosocomial infection. The tests were carried out by three methods: the test object method, as well as suspension and adsorption methods. The minimum effective concentration and exposure time of the preparation, which ensure the complete destruction of these microorganisms, have been established. The effect of organic substances and neutralizers on the antimicrobial activity of the preparation was studied. «Sulfonol» (a mixture of stereoisomers of the sodium salt of alkylbenzenesulfonic acid) at a concentration of 0,2?% and ten percent milk were used as neutralizers, and 10?% horse serum was used as protein. The concentration of the preparation and the exposure time required for the complete disinfection of dishes, glass and metlakh tiles infected with S. aureus and E. coli were determined. Disinfection of surfaces is achieved by treatment with a 3?% solution of the preparation during an exposure of 60 minutes, and dishes – with 3–5?% solutions for 45 minutes. It has been established by the thermoanalytical method of analysis that the preparation is stable at normal temperature and does not change its physicochemical properties during storage for 18 months or more. The preparation is recommended as a disinfectant for medical purposes. Keywords: 1,3-oxazolidine derivatives, 3-(?-hydroxyethyl)-1,3-oxazolidine, 3-(?-hydroxyoxymethylethyl)-1,3-oxazolidine, antimicrobial activity, bactericidal activity, nosocomial infection, bacteria, S. aureus, E. coli, disinfection, disinfectant.
二乙醇胺与甲醛在初始试剂比例为2∶3时的相互作用3分别获得了3-(?-羟乙基)-1,3-恶唑烷及其甲基衍生物- 3-(?-羟氧甲基乙基)-1,3-恶唑烷的等摩尔混合物的制剂。本文介绍了该制剂对微生物的抗菌活性的研究结果-金黄色葡萄球菌,大肠杆菌,作为引起医院感染的最耐药的细菌物种。试验采用试验对象法、悬浮法和吸附法三种方法进行。已经确定了制剂的最低有效浓度和暴露时间,以确保这些微生物的完全破坏。研究了有机物质和中和剂对该制剂抑菌活性的影响。磺胺醇(烷基苯磺酸钠盐的立体异构体混合物)浓度为0.2 ?%和10%的牛奶被用作中和剂,10%的牛奶被用作中和剂。蛋白质采用%马血清。测定了对感染金黄色葡萄球菌和大肠杆菌的餐具、玻璃和面砖进行彻底消毒所需的制剂浓度和暴露时间。表面消毒是用3?%溶液的制备过程中暴露60分钟,盘子- 3-5 ?%溶液浸泡45分钟。经热分析分析方法确定,该制剂在常温下稳定,在18个月以上的贮存期间,其理化性质不发生变化。该制剂推荐作为医疗用途的消毒剂。关键词:1,3-恶唑烷衍生物,3-(?-羟乙基)-1,3-恶唑烷,3-(?-羟基氧甲基乙基)-1,3-恶唑烷,抗菌活性,杀菌活性,医院感染,细菌,金黄色葡萄球菌,大肠杆菌,消毒,消毒剂
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引用次数: 0
Experimental evaluation of Yersinia pseudotuberculosis bacteria sensitivity to antimicrobial compositions based on copper nanoparticles in in vitro and in vivo experiments 假结核耶尔森菌对铜纳米颗粒抗菌组合物敏感性的体外和体内实验评价
Pub Date : 2023-03-01 DOI: 10.35411/2076-457x-2023-1-5-23
G. Frolov, Abdulla Abumalik Abdukadirov ugli, I. Lundovskikh, Anna Yuryevna Mironina, K. I. Gurin, I. Pogorelsky, Aleksandr Victorovich Mironin, K. E. Gavrilov
The paper presents the results of the antibacterial efficacy of disinfecting compositions based on copper nanoparticles and its oxides in antiseptic solutions of cetylpyridinium chloride and benzalkonium chloride against the causative agent of pseudotuberculosis Y. pseudotuberculosis of in vitro and in vivo experiments. Using the bacteriological method and the results of electron microscopy, high bactericidal activity of disinfecting compositions has been proven. Anti-yersiniosis activity and safety of both compositions were revealed in experimental pseudotuberculosis of conventional white mice. Simultaneous oral administration of the compositions under study to experimental animals limits the initiation and development of the infectious process and prevents the death of laboratory animals. Possible mechanisms of enhancing the biocidal activity of the tested disinfecting compositions are considered. Keywords: pseudotuberculosis, microorganisms, biocidal activity, antiseptic, nanoparticles, zeta potential.
本文介绍了铜纳米颗粒及其氧化物消毒组合物在氯化十六烷基吡啶和氯化苯扎氯铵防腐液中对致病菌假结核杆菌的体外和体内抑菌效果。利用细菌学方法和电子显微镜检查结果,证实了消毒组合物具有较高的杀菌活性。实验结果表明,两种组合物在常规小鼠假结核实验中抗耶尔森菌病的活性和安全性。将所研究的组合物同时口服给药给实验动物,限制了感染过程的开始和发展,并防止实验动物的死亡。考虑了提高所测消毒组合物的杀生物活性的可能机制。关键词:假结核,微生物,杀菌活性,防腐剂,纳米颗粒,zeta电位。
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引用次数: 0
Reversion of susceptibility to insecticides of male German cockroach of resistant strains during long-term cultivation under laboratory conditions 实验室条件下长期培养抗药株雄蜚蠊对杀虫剂敏感性的逆转
Pub Date : 2023-03-01 DOI: 10.35411/2076-457x-2023-1-41-46
O. Eremina, V. V. Olifer, T. A. Davlianidze
Under laboratory conditions, resistance to insecticides from various chemical classes was assessed on several strains of the German cockroach Blattella germanica obtained from populations collected at sites in Moscow, Kaluga, Sverdlovsk and Moscow regions. It has been established that when applied topically, German cockroaches of all strains are highly resistant to cypermethrin (RR = 133–>4000?), fipronil (RR = 14–61?), chlorpyrifos (RR = 15–40?), weakly tolerant to thiamethoxam and clothianidin (RR = 2–5?). After breeding in an insectarium without insecticide press for several generations, the resistance of M1 strain to cypermethrin decreased by 1.2 times, in U1, M9 and OBN strains resistance remained at the same high level, in U8 strain decreased by 20 times; the resistance to fipronil in M1, U1 and OBN strains decreased by 1.4, 1.7 and 5.0 times, respectively. The level of susceptibility to chlorpyrifos decreased by 1.2–2.2 times, depending on the strain. Tolerance of cockroaches to thiamethoxam and clothianidin remained at the same level. The mechanisms of resistance of the German cockroach to insecticides are discussed. Keywords: pyrethroids, neonicotinoids, organophosphorus compounds, phenylpyrazoles.
在实验室条件下,对从莫斯科、卡卢加、斯维尔德洛夫斯克和莫斯科地区采集的德国小蠊种群中获得的几种菌株对不同化学类杀虫剂的抗性进行了评估。结果表明,局部施用时,所有品系德国蜚蠊对氯氰菊酯(RR = 133 ~ >4000?)、氟虫腈(RR = 14 ~ 61?)、毒死蜱(RR = 15 ~ 40?)均有高抗性,对噻虫嗪和噻虫胺(RR = 2 ~ 5?)均有弱抗性。在无杀虫剂压制的食虫室中繁殖数代后,M1菌株对氯氰菊酯的抗性下降了1.2倍,U1、M9和OBN菌株抗性保持在较高水平,U8菌株抗性下降了20倍;M1、U1和OBN菌株对氟虫腈的耐药性分别下降1.4倍、1.7倍和5.0倍。根据菌株的不同,毒死蜱的敏感性降低了1.2-2.2倍。蟑螂对噻虫嗪和噻虫胺的耐受性基本保持不变。讨论了德国蜚蠊对杀虫剂产生抗药性的机理。关键词:拟除虫菊酯,新烟碱类,有机磷化合物,苯吡唑
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引用次数: 0
Experience in the practical use of a multifunctional disinfection station for the purpose of additional measures during fighter disinsection measures on the example of bed bugs 多功能消毒站的实际使用经验,以臭虫为例,在战斗消毒措施期间采取额外措施
Pub Date : 2023-03-01 DOI: 10.35411/2076-457x-2023-1-52-55
N. A. Vatolina, L. V. Lyalina, A. Datskevich, Lyubov Ivanovna Kabay, T. V. Osmirko
Today, disinfection institutions, having a small selection of insecticide active ingredients and a huge selection of mix insecticide preparations to combat bed bugs, face the problem of ensuring effective processing of objects of different categories. Keywords: preventive disinfection, fighter disinsection measures, disinsection, chamber disinfection (disinsection), bed bugs, tropical bed bugs, disinsection equipment.
如今,消毒机构在对付臭虫时,杀虫剂有效成分的选择很少,而混合杀虫剂制剂的选择却很多,它们面临着如何保证对不同类别的对象进行有效处理的问题。关键词:预防性消毒,歼灭措施,消毒,室内消毒(除虫),臭虫,热带臭虫,除虫设备。
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引用次数: 0
Safety of using disinfectants based on sodium hypochlorite in practice 次氯酸钠类消毒剂在实践中的安全性研究
Pub Date : 2023-03-01 DOI: 10.35411/2076-457x-2023-1-23-30
G. P. Pankratova, M. V. Bidevkina, Z. К. Shaikhutdinova
The parameters of toxicometry of disinfectants based on sodium hypochlorite obtained by chlorination of an aqueous solution of caustic soda (31 agents) and by electrochemical method – 10 anoliths were established. Based on the determination of the chlorine content in the air, recommendations are given for the safe use of these disinfectants: for surface treatment by wiping and disinfection of air and surfaces by aerosol spraying using a special device – an aerosol generator that provides dispersion of aerosol particles of 5–50 microns. A working solution of sodium hypochlorite with an active chlorine content of 0.015?% is recommended for use by wiping in the presence of people. Keywords: disinfectants, sodium hypochlorite, anolytes, toxicity, irritant effect, inhalation hazard, aerosol, laboratory animals.
建立了以次氯酸钠为基础的消毒剂(31种药剂)经苛性钠水溶液氯化处理后的毒理测定参数和电化学法- 10块石法的毒理测定参数。根据对空气中氯含量的测定,对这些消毒剂的安全使用提出了建议:对空气和表面进行表面处理,通过使用一种特殊装置(一种气溶胶发生器,可提供5-50微米的气溶胶颗粒分散)进行气溶胶喷洒,对空气和表面进行擦拭和消毒。次氯酸钠的工作溶液,有效氯含量为0.015?%建议在有人在场的情况下使用。关键词:消毒剂,次氯酸钠,解毒剂,毒性,刺激作用,吸入危害,气溶胶,实验动物。
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引用次数: 0
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Disinfection affairs
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