Eksperimentalna meditsina i morfologiia最新文献

Experimental studies were carried out on white male sexual mature healthy rats with induced alloxan diabetes, to clarify the role of dopaminergic system in the regulation of functions of the insular apparatus of the pancreas. The experiments were performed by the following drugs: bromrcripine, levodopa, a combination of revodopa plus carbidopa and haloperidol. Parameters of carbohydrate metabolism were determined in the course of acute and chronic experiments. The results sustained the hypothesis for participation of dopaminergic system in the regulation of production and secretion of insulin and respectively in the control of carbohydrate metabolism. Independent studies were carried out on white rats with alloxan diabetes after combined administration of dopaminergic pharmacological drugs and sulphanylureic preparation (SUP)-glybenclimide. The mechanisms of interaction after the combined administration of the investigated drugs under the conditions of experimental diabetes mellitus were clarified. The obtained results supplement data, in support of the hypothesis for the role of dopamine as a neuromediator in the regulation of the insular apparatus of the pancreas.

Liposomal systems were prepared for oral usage of narcotic analgesics morphine and phentanyl. The systems were examined for homogeneity in respect to seize size and form of liposomal structures by a diffraction-laser method and electron microscopy. The liposomal drug forms were studied on biological experiment as the change in the body temperature of rats was described. It was established that in rats oral usage of liposomal systems with morphine respectively with phentanyl caused significantly better effect in comparison with active substances, not taken in liposomes.

The change of ceruloplasmin level of blood serum of guinea-pigs was studied on the 12th h after irradiation with gamma-rays from 60Co, using doses of 1-10 Gy, power of the dose of 0.68 Gy/min. The enzyme level was raised by the increase of the irradiation dose as it was reduced by the dose of 10 Gy in comparison with the control animals. This was probably due to inactivation of the molecules of ceruloplasmin by radicals and peroxides, obtained by this hyperlethal dose. Sera proteins showed a change in the increase of the dose: a reduction of albumins and an increase in beta- and gamma-globulins. Alpha-2-globulins, with which ceruloplasmin moved electrophoretically at low doses, showed slowly a slow increase in comparison with the control value, but at high doses-phase globulins. Oxidase activity of ceruloplasmin was determined by 01 todianisidine, but sera proteins-by agarose electrophoresis. The level of s ceruloplasmin in animals, irradiated by LD50 = 4.1 Gy, showed chronological change-an increase at early periods, maximum on the 12th h and a reduction at late periods close to the normal range. The level of serum copper followed the change in the enzyme level by analogy.

The results from morphological studies on liver, lung, heart, spleen, intestine, brain, skin, testes (ovaries), obtained in the end of the first and third month of animals, treated with pharmapentoxiphyline (PP), are described. The experiments were carried out on 312 white rats (male and female), divided into 2 series: I series--oral usage of the preparation in doses of 100, 300 and 6 mg/kg, II series--intraperitoneal administration in doses of 30, 75 and 150 mg/kg. It was established that PP, administered for a period of 3 months orally in a dose of 100 mg/kg and intraperitoneally in a dose of 30 mg/kg did not induced changes in the structure of all examined organs. Slight dystrophic changes in liver, lung and spleen occurred in the end of the third month under the influence of an oral dose of 300 mg/kg and an intraperitoneal dose of 75 mg/kg. The obtained results are discussed in connection with the pharmacological action of drug-peripheral vasodilatator.

Results from the study on acute, subacute and chronic toxicity of the therapeutic preparation pharmapentoxiphyline (PP), produced by Pharmachem, are described. The experiments were carried out on 312 male and female white rats. According to the limiting index of LD50 with, Oral dose of (1220 mg/kg of body mass for male rats and 1050 mg/kg for female rats) and intraperitoneal PP dose of PP (230 mg/kg for female rats and 235 mg/kg for female rats) it was included into IV class of slightly toxic compounds. Doses of 100, 300 and 600 mg/kg/oral administration) and 30, 75 and 150 mg/kg (intraperitoneal administration) were used in subacute and chronic experiments. Integral, hematological and biochemical methods and parameters were applied for evaluation of the toxic effect. There was no hemato-, hepato- and nephrotoxicity after oral and intraperitoneal administration of PP in doses of 10 and 30 mg/kg for a period of 3 months. Some recommendations, which should be taken into consideration during continuous treatment with PP, are given on the basis of a complex evaluation of the results from the hematological parameters.

The results from artificial pneumothorax in 25 rabbits with intrapleural administration of Uropolin (10 rabbits) as well as of 10% solution of NACl (7 rabbits) were described. Thoracotomy was made on 7th, 14th and 12 21st day and the parietal, diaphragmatic and visceral pleura with underlaying tissues were examined. Proliferation of the mesothelium [correction of mesother] and growth of the connective tissue, which were more manifested in rabbits treated with Uropolin, were established histologically. The authors think that pathohistological examinations confirm the clinical observed favourable effect of intrapleural administration of Uropolin for obliteration of the pleural cavity.

A cytofluorimetric method for determination of the reactivity of antimyeloma MkAt 225,28S with myeloma cells and blood elements is described in the present article. The obtained results show that the antibody possesses high specific activity and does not bind with normal human lymphocytes, monocytes, granulocytes, thrombocytes and erythrocytes in the examined dilutions. The method is suitable for control of cross reactivity with blood cells of MkAt preparations for usage in subjects.

The author investigated the changes in the morphology of germinative and Sertoli's cells during prespermatogenesis of the rat. This problems were studied by morphometric investigations in 20 rats of Wistar strain, divided into age groups: newborns, 5, 10 and 15 postnatal days. The morphofunctional characteristic of these two types of cellular populations in immature rats showed existence of parallelism in the proliferation of these cells, the level of organization and interrelationships between them, which was of substantial significance for creation and further maintenance of an optimal microenvironment, needed for differentiation of sex cells.