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Quality Characteristics and Ultra Structural Changes of Restructured Buffalo Meat Slices with Flaxseed Flour as Binder: A Novel Value Added Technology 以亚麻籽粉为粘结剂的水牛肉片的品质特性及超微结构变化——一种新的增值技术
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3035
G. V. Bhaskar Reddy, K. Viswa natha Reddy, A. P
In India, spent buffaloes are the main contributors of meat, which is tough and low priced and poor demand in the market. In this context, a new technological intervention is needed for restructuring of meat to enhance value-added meat production. Accordingly, an investigation was carried out to analyze the efficacy of different levels of flaxseed flour (C, T1, T2, and T3) as a novel binder for making of Restructured Buffalo Meat Slices (RBMS). The results revealed that T3 (RBMS added with 6% flaxseed flour) demonstrated significantly (P<0.01) higher cooking yield, water-holding capacity (WHC), batter stability and lower diameter shrinkage than control and RBMS incorporated with 2 % and 4 % flax seed flour. None of the treatments had significant influence on pH, total protein extractability, collagen content and solubility of RBMS. Moreover, T3 revealed significantly (P<0.01) higher moisture, total protein, total fat and total ash content than RBMS formulated with other levels of flax seed flour. Conversely, significantly lower in hardness, springiness, chewiness and higher in cohesiveness than other treatments. However, T3 influenced the colour scores, though at different degrees. Ultra structural images of cooked samples displayed the protein matrix between myofibrils which indicates gel network of flaxseed gum and proteins, subsequently caused more cohesiveness. This implies that the flaxseed flour acted as excellent novel binder for binding meat pieces. T3 has proven significantly greater sensory scores than other treatments. Thus, it is clearly evident that T3 has improved most of the quality attributes of buffalo meat and can be recommended as a novel value added technology for meat industry.Keywords: Buffalo meat; Flaxseed flour; Quality characteristics; Restructuring; Ultra structure
在印度,用过的水牛是肉类的主要来源,肉类质地坚硬,价格低廉,市场需求量大。在这种情况下,需要对肉类结构进行新的技术干预,以提高肉类的附加值生产。因此,进行了一项研究,以分析不同水平的亚麻籽粉(C、T1、T2和T3)作为一种新型粘合剂用于制备重组水牛肉片(RBMS)的功效。结果表明,T3(添加6%亚麻籽粉的RBMS)比对照和添加2%和4%亚麻籽粉(RBMS)显著(P<0.01)提高了蒸煮产量、持水性(WHC)、面糊稳定性和较低的缩径率。所有处理均未对RBMS的pH值、总蛋白提取率、胶原蛋白含量和溶解度产生显著影响。此外,T3显著(P<0.01)高于用其他水平的亚麻籽粉配制的RBMS的水分、总蛋白、总脂肪和总灰分。相反,与其他处理相比,硬度、弹性、耐嚼性显著降低,内聚性更高。然而,T3对颜色分数有不同程度的影响。烹饪样品的超结构图像显示了肌原纤维之间的蛋白质基质,这表明亚麻籽胶和蛋白质的凝胶网络,随后引起了更多的内聚性。这意味着亚麻籽面粉是一种很好的新型肉块粘合剂。T3的感觉评分明显高于其他治疗。因此,很明显,T3改善了水牛肉的大部分质量属性,可以推荐作为肉类工业的一种新的增值技术。关键词:水牛肉;亚麻籽面粉;质量特性;重组;超结构
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引用次数: 0
Simultaneous green synthesis of Magnetite-Nanoparticles MNPs using microalgae Spirulina sp. for antibacterial activity 利用具有抗菌活性的微藻螺旋藻同时绿色合成磁性纳米颗粒MNPs
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3033
Sewgil Saaduldeen Anwer
 Biosynthesize of Magnetic Nanoparticles MNPs is the environmentally friendly synthesis of nanoparticles that can be used as an alternative to commercially available antibiotics. The present study aimed to determine the ability of biosynthesized magnetic nanoparticles of Spirulina sp. for antibacterial activity. Microalgae isolated from the Gomaspan river cultured on BG11 medium and, is identified using morphology and molecular method and the optimum growth rate of microalgae studied, the biomass used to synthesize of MNPs then was characterized by a visible color change and Scanning electron microscope SEM, FTIR with XRD. Antimicrobial activity of Spirulina sp. and biosynthesize of MNPs. studied using different extracts (ethanol, methanol and Diethyl ether) against growth of Salmonella Typhi, Streptococcus pyogenes, Escherichia coli and pseudomonas aerogenes by disc diffusion and Minimum inhibitory concentration methods. The antibacterial activity from microalgae Spirulina sp. and biosynthesized MNPs from Spirulina sp. showed to inhibit growth of bacteria with both methods and the higher inhibition zone showed as (30-37mm). The minimum inhibition concentration showed with ethanol extract (125-500 µg/l). The current study is first report an eco-friendly and convenient method for the synthesis of MNPs using Microalgae Spirulina sp. extracts. This biosynthetic process might be useful pharmaceuticals, and medicine treatment of pathogenic bacteria.Keywords: Nanoparticle, Magnetic, Spirulina, Antibacterial, solvent extract
磁性纳米颗粒MNPs的生物合成是一种对环境友好的纳米颗粒合成,可作为市售抗生素的替代品。本研究旨在测定螺旋藻生物合成的磁性纳米颗粒的抗菌活性。从Gomaspan河中分离出的微藻在BG11培养基上培养,利用形态学和分子生物学方法对微藻进行鉴定,并研究了微藻的最佳生长速率,然后用可见颜色变化和扫描电子显微镜SEM、FTIR和XRD对用于合成MNP的生物量进行了表征。螺旋藻的抗菌活性及MNPs的生物合成。采用纸片扩散法和最小抑菌浓度法研究了不同提取物(乙醇、甲醇和乙醚)对伤寒沙门氏菌、化脓性链球菌、大肠杆菌和产气假单胞菌生长的抑制作用。微藻Spirulina sp.和螺旋藻生物合成的MNPs的抗菌活性均显示出抑制细菌生长的作用,较高的抑制区为(30-37mm)。乙醇提取物的最低抑制浓度(125-500µg/l)。本研究首次报道了一种利用螺旋藻提取物合成MNPs的环保、方便的方法。这种生物合成过程可能是有用的药物,以及治疗病原菌的药物。关键词:纳米粒子,磁性,螺旋藻,抗菌,溶剂提取物
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引用次数: 0
A New Isoflavone from Lomariopsis guineensis (Underw.) Alston 金合欢中一种新的异黄酮
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3034
N. Al-Tannak, Abdullah Al Ali, E. Santali, C. J. Clements, A. I. Gray, J. Igoli, Jamal Abdul, Kuwait Nasser St
Aim/background: Lomariopsis guineensis (Underw.) Alston is an epiphytic climbing fern. It is widely distributed in Africa where it is also used in traditional medicine and as food. There are no previous reports of any constituents of the plant, hence this study to isolate any phytoconstituents. Method: The ethyl acetate extract of the leaves was subjected to column chromatography and isolated constituents were characterized using nuclear magnetic resonance and mass spectrometry. Results: Three compounds were isolated and identified as cycloartenol, pheophytin A and a new isoflavone (5, 7-dihydroxy-4′ methoxy-6,8-dimethylisoflavone). Conclusion: Three phytochemicals including a new isoflavone are reported from the plant for the first time.Keywords: Isoflavone; Lomariopsis guineensis; pheophytin A; cycloartenol; antitrypanosomal assay
目的/背景:几内亚Lomariopsis guineensis (Underw.)是一种附生攀缘蕨类植物。它广泛分布在非洲,在那里它也被用于传统药物和食物。该植物的化学成分未见文献报道,因此本研究旨在分离该植物的化学成分。方法:采用柱层析法提取乙酸乙酯提取物,并采用核磁共振和质谱法对分离成分进行表征。结果:分离得到3个化合物,鉴定为环蒿醇、叶绿素A和一个新的异黄酮(5,7 -二羟基-4′甲氧基-6,8-二甲基异黄酮)。结论:首次从该植物中分离到3种植物化学物质,其中包括1种新的异黄酮。关键词:大豆异黄酮;Lomariopsis guineensis;脱镁叶绿素;cycloartenol;antitrypanosomal化验
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引用次数: 0
Effect of fungicide treatment and nitrogen fertilisation on the yield of two breeding types of winter oilseed rape cultivars 杀菌剂处理和施氮对两种冬油菜品种产量的影响
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3011
M. Wójtowicz, ,. A. Wójtowicz, E. Jajor, M. Korbas
The effect of three fungicide treatment programmes and the level of spring nitrogen fertilisation on the seed yield of two types ofcultivars of Brassica napus L. sown at two different seeding rates was studied in a field experiment. The subject of the study was anopen-pollinated cultivar ‘Casoar’ and a restored hybrid cultivar ‘Visby’. Three plant protection programmes, two levels of spring nitrogenfertilisation (160 and 220 kg N⋅ha1), and two different seeding rates for each cultivar (‘Visby’—50 and 70 seeds⋅⋅m2; ‘Casoar’—60 and 80 seeds⋅m2) were included. The most intensive protection programme comprised three fungicide treatments: first in autumn at the six-leaves-unfolded stage—BBCH 16, second in spring at the stem elongation stage—BBCH 33, and third at the full flowering stage—BBCH 65. One of two less intensive programmes of plant protection included fungicide application in autumn at the sixleaves-unfolded stage—BBCH 16 and at the full flowering stage—BBCH 65, while the second included fungicide application in spring at the stem elongation stage—BBCH 33 and at the full flowering stage—BBCH 65. The effectiveness of the protection programmes and nitrogen fertilisation was influenced by the intensity of abiotic stress factors. The average yield from the plots protected against pathogens was significantly higher than that from the untreated plots. The increase of nitrogen fertilisation from 160 to 220 kg⋅ha1 also caused a significant increase of average seed yield. The yield of cultivar ‘Visby’ was higher and less dependent on the seeding rate compared to cultivar ‘Casoar’.Keywords: Winter oilseed rape; Cultivars; Disease control; Nitrogen fertilisation; Seeding rate; Sclerotinia sclerotiorum;           Leptosphaeria spp.; Alternaria spp. Botrytis cinerea.
通过田间试验,研究了三种杀菌剂处理方案和春季氮肥水平对两种不同播种率下甘蓝型油菜两个变种种子产量的影响。这项研究的对象是一个授粉的按蚊品种“Casoar”和一个恢复的杂交品种“Visby”。包括三个植物保护计划,两个春季氮肥水平(160和220 kg N·ha1),以及每个品种的两种不同播种率(“Visby”——50和70种子·m2;“Casoar”——60和80种子·m2)。最密集的保护方案包括三种杀菌剂处理:第一种是在秋季六叶展开期BBCH 16,第二种是在春季茎伸长期BBCH 33,第三种是在全花期BBCH 65。两个强度较小的植物保护方案之一包括在秋季六叶展开期施用杀菌剂(BBCH 16)和在盛花期施用杀菌素(BBCH 65),而第二个方案包括在春季茎伸长期施用杀菌药(BBCH 33)和在全花期施用杀真菌剂(BBCH65)。保护方案和氮肥施用的有效性受到非生物胁迫因素强度的影响。受病原体保护的地块的平均产量显著高于未经处理的地块。施氮量从160公斤增加到220公斤,也显著提高了平均种子产量。与品种“Casoar”相比,品种“Visby”的产量更高,对播种率的依赖性更小。关键词:冬油菜;栽培品种;疾病控制;氮肥;播种率;核盘菌;纤球菌属。;链格孢。灰葡萄孢。
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引用次数: 0
Optimization of cotton irrigation management for different climatic conditions using the CROPGRO-Cotton model 应用CROPGRO棉花模型优化不同气候条件下的棉花灌溉管理
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3029
Junfeng Gao, Bao-Ping Zhou, Zi-Ya Tan
To study the feasibility of CROPGRO_cotton model in the optimization of cotton irrigation management under different climatic conditions, the empirical values corresponding to 25%, 50% and 75% of the empirical frequencies of precipitation were obtained by ranking the frequency fitness line of precipitation data during 20 years of cotton fertility at the experimental site, and then the years corresponding to three empirical frequencies were selected as typical years: 2015 (abundant water year), 2001 (flat water year), and 2006 (dry water year). By combining cotton fertility stages, irrigation frequency and irrigation amount, 21 irrigation regimes (T1~T21) were identified and simulated using the calibrated DSSAT model for cotton irrigation regimes under the three precipitation year types, and the results showed that under the best combination of irrigation usage, yield and water utilization, 2015 (abundant water year), 2001 (flat water year), and T20 irrigation management should be selected in 2006 (dry water year).Under the three typical years, the effect of temperature change on cotton yield and water utilization was investigated, and it was found that the increase of temperature would reduce cotton yield and water utilization, but a reasonable irrigation management would reduce the negative effect of climate change on cotton yield and water utilization,According to the simulation results under different situations, T20 irrigation management can minimize the yield variation range under temperature change, and has a high water utilization rate, which has good applicability.Keywords: Cotton; DSSAT model; Irrigation management; Typical years; Applicability
为了研究CROPGRO_cotton模型在不同气候条件下优化棉花灌溉管理的可行性,通过对试验点棉花生育20年降水数据的频率适应度线进行排序,得到了对应于25%、50%和75%的经验降水频率的经验值,然后选取三个经验频率对应的年份作为典型年份:2015年(丰水年)、2001年(平水年)和2006年(枯水年)。通过结合棉花肥力阶段、灌溉频率和灌溉量,使用校准的DSSAT模型对三种降水年型下的21种灌溉方式(T1~T21)进行了识别和模拟,结果表明,在灌溉利用、产量和水分利用的最佳组合下,2015年(丰水年),2001年(平水年)和T20灌溉管理应选择在2006年(枯水年)。在这三个典型年份下,研究了温度变化对棉花产量和水分利用的影响,发现温度升高会降低棉花产量和水利用率,但合理的灌溉管理可以减少气候变化对棉花产量和水分利用的负面影响。根据不同情况下的模拟结果,T20灌溉管理可以最大限度地缩小温度变化下的产量变化范围,具有较高的水分利用率,具有良好的适用性。关键词:棉花;DSSAT模型;灌溉管理;典型年份;适用性
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引用次数: 0
Impact of pre-sowing red laser irradiation of corn seeds on quality and quantity of harvest yield 播前红色激光照射玉米种子对收获产量质、量的影响
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3025
Rivera-Talamantes Carlos Francisco de Jesus, Gonzalez-Lopez Gabriela, Michtchenko Alexandre
As the population increases, more people need to be fed. In order to find a physical method that influence the corn production, this work is dedicated to evaluating the impact of pre-sowing red laser irradiation of corn (Zea mays L.) seeds on harvest yield. The aim was to analyze the influence of red laser radiation on corn seeds, on the quantity and quality of yield, on large-scale and open field production. It is hypothesized that at least one red laser irradiation treatment could improve corn crop yield. In radiation, a red laser diode at 660 nm with a power of 100 mW was used, two radiation densities were used (D1: 2 mW cm-2 and D2: 4 mW cm-2), applied during 4 exposure times (T1: 15, T2: 30, T3: 60 and T4: 120 s) and a control group without treatment (C). A random arrangement was used, in a 2 x 4 factorial scheme, totaling 8 treatments and control, with four replications. The data were subjected to an ANOVA and the means were compared with the Tukey test (HSD; p ≤ 0.05). The D2-T4 treatment produced the most significant impact concerning the control, improving yield by 19±1% (3 t ha-1), cob length 18±1%, cob diameter 22±3%, weight (16±2%) and corn kernels size (14±2%). We also find negative effects on yield, D2-T1 decreased crop yield around 16±1% (2.24 t ha-1). The results show a way to include this technique as a technique that can to increase or decrease corn yield.Keywords: Cob length; Maize; open field; Thousand kernel weight.
随着人口的增加,需要养活的人也越来越多。为了找到一种影响玉米产量的物理方法,本工作致力于评估播前红色激光照射玉米(Zea mays L.)种子对收获产量的影响。分析了红色激光辐射对玉米种子的影响、对产量数量和品质的影响、对大规模大田生产和露天大田生产的影响。假设至少有一种红色激光照射处理可以提高玉米作物产量。在辐射方面,使用功率为100 mW的660 nm红色激光二极管,使用两种辐射密度(D1: 2 mW cm-2和D2: 4 mW cm-2),在4个暴露时间(T1: 15、T2: 30、T3: 60和T4: 120 s)和未处理的对照组(C)中施加辐照。采用随机排列,按2 × 4因子方案,共8个处理和对照,4个重复。对数据进行方差分析,并用Tukey检验(HSD;P≤0.05)。D2-T4处理对对照的影响最为显著,产量提高19±1% (3 t ha-1),穗轴长提高18±1%,穗轴直径提高22±3%,玉米重提高16±2%,籽粒大小提高14±2%。我们还发现了对产量的负面影响,D2-T1使作物产量下降约16±1% (2.24 t ha-1)。结果显示了一种将该技术作为一种可以增加或减少玉米产量的技术的方法。关键词:棒材长度;玉米;开放的领域;千粒重。
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引用次数: 0
Weed management with Triafamone herbicide in transplanted rice ecosystem Triafamone除草剂在水稻移植生态系统中的杂草管理
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3027
P. Arthanari
Field experiments were conducted during Rabi 2018-19 (September to January) and Kharif 2019 (May to September). Medium duration rice cv. CO(R) 51 was used as a test variety at Tamil Nadu Agricultural University, Coimbatore, India to study the effect of Triafamone on broad spectrum weed control in transplanted rice. Triafamone was applied at two times. First set of treatments (30, 40,50 and 100 g/ha) applied two to three leaf stage of weeds and second set treatments imposed (30, 40,50 and 100 g/ha) at three days after transplanting of the rice crop. These treatments were compared with Pyrazosulfuron ethy 10% WP @ 15 g/ha, Pretilachlor 5% EC @ 750 g/ha, hand weeding @ 20 and 40 days after transplanting and unweeded control. The major grasses were Echinochloa crus-galli (L.) and E. colonum (L.) while the sedge weed included Cyperus nutans (L.). Among the broad leaved weeds Eclipta alba (L.) and Bergia ammanioides were the dominant species. In both the seasons of the study, lesser population of grass was recorded with the pre and early post emergence application of triafamone herbicide 40 g a.i. ha-1 (1.41 and 1.41 per m-2 at 28 and 42 DAA for both the seasons respectively) and it was closely followed by application of triafamone herbicide 50 g a.i. ha-1 and application of PE Pretilachlor at 0.75 kg a.i. ha-1 and EPOE Pyrazosulfuron ethyl at 15 g a.i. ha-1. The same trend was observed for broad leaved weeds and their weed dry weights. Application of triafamone herbicide at 40 g a.i. ha-1 as pre emergence and early post emergence application recorded highest weed control efficiency of 100, 97.7 and 99.6 % and 97.8% at 42 DAA during both years of experimentation and also resulted in higher grain yield (8052 and 8182 kg ha-1 during rabi 2018-19 and 9018, 9175 kg ha-1 during Kharif 2019). Further, it is noticed that, there was no residual toxicity of herbicide to the succeeding crops. Hence, application of Triafamone 40 g a.i. ha-1 either pre or early post emergence herbicide has higher productivity and no residues in transplanted rice ecosystem. Key words: Triafamone, efficacy, weeds and transplanted rice ecosystem
野外实验在2018-19年拉比(9月至1月)和2019年哈里夫(5月至9月)期间进行。以印度哥印拜陀泰米尔纳德邦农业大学的中效水稻品种CO(R)51为试验品种,研究了三唑酮对移植水稻广谱除草的影响。Triafamone分两次使用。第一组处理(30、40、50和100克/公顷)施用二至三叶杂草期,第二组处理在水稻作物移植后三天施用(30、40,50和100克每公顷)。将这些处理与吡嘧磺隆乙醇10%可湿性粉剂15g/ha、预甲草胺5%EC 750g/ha、人工除草20d和40d以及未播种对照进行比较。主要杂草为鹅掌楸(Echinochloa crus galli,L.)和大肠杆菌(E.colonum,L.),莎草为莎草(Cyperus nutans,L.)。在研究的两个季节中,在羽化前和羽化后早期施用40 g a.i.ha-1(两个季节在28和42 DAA时分别为1.41和1.41/m-2),草的数量较少,紧随其后的是施用50 g a.i.ha-1和0.75 kg a.i.ha-1的PE预甲草胺和15 g a.i.a-1的EPOE吡嘧磺隆乙基。对于阔叶杂草及其杂草干重也观察到了相同的趋势。在两年的试验中,以40g a.i.ha-1作为出苗前和出苗后早期施用三唑酮除草剂,在42DAA时的杂草控制效率分别为100%、97.7%、99.6%和97.8%,还带来了更高的粮食产量(2018-19年拉比期间为8052和8182 kg ha-1,2019年哈里夫期间为90189175 kg ha-1)。此外,值得注意的是,除草剂对后续作物没有残留毒性。因此,在出苗前或出苗后早期施用Triafamone 40g a.i.ha-1除草剂具有较高的生产力,并且在移植水稻生态系统中没有残留。关键词:三唑酮、药效、杂草与水稻移植生态系统
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引用次数: 0
Correlations of flavonoids content and antioxidant activity in bee honey from Bosnia and Herzegovina 波斯尼亚和黑塞哥维那蜂蜜中黄酮类化合物含量与抗氧化活性的相关性
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3009
I. Tahirovića, H. Kurtagić, N. Smječanin
In this study, it was performed identification and quantification of flavonoids (apigenin, chrysin, hesperetin, kaempferol, luteolin, naringenin, and quercetin) and flavonoid glycosides (rutin and vitexin) in total 49 samples of five different honey types from Bosnia and Herzegovina: meadow honey (MH, 22 samples), forest (FH, 10), acacia (AH, 7), chestnut (CH, 5), and heather honey (HH, 5). Additionally, evaluation of correlations between FC and total hydrophilic antioxidant score (antioxidant activity against both: ROO· + OH·) in supernatants (s) and in bulk (noncentrifuged) solution (b) of these honey types was performed. Moreover, correlations between flavonoids content (FC) and previously reported antioxidant activity against both peroxyl and hydroxyl free radicals (AC(ROO·) and AC(OH·)) for the same honey samples was examined. High performance liquid chromatography with photodiode array detector (HPLC-DAD) and isocratic elution mode was used as method of analysis. Flavonoids were extracted by solid phase extraction (SPE). The average contents of three flavonoids (chrysin, naringenin, and luteolin) in MH were statistically higher than in AH (p**<0.01). Also, the average content of naringenin in FH was statistically higher than in CH (p*<0.05). We observed a high (positive) linear correlation between FC and AC(ROO·) in s of four honey types (FH, AH, HH, CH) (R2=0.920). If we correlate FC and AC(ROO·)s of three honey types (FH, AH, HH), linearity is very high (R2=0.968), and for FH, AH, CH linearity is complete. The correlation between FC and AC(ROO·) in b of the same honey types is similar, but lower. The correlation does not exist between FC and AC(OH·) neither in s nor in b of five or four honey types, but for FC to both (AC(OH·)s and AC(OH·)b) of three honey types (FH, AH, CH), linearity is moderate (R2=0.732 and R2=0.696, respectively).Keywords: antioxidant activity, correlations, flavonoids, honey, HPLC-DAD.
在本研究中,对波斯尼亚和黑塞哥维那五种不同蜂蜜类型的总共49个样品中的黄酮类化合物(芹菜素、白杨素、橙皮素、山奈酚、木犀草素、柚皮素和槲皮素)和黄酮苷类化合物(芦丁和vitexin)进行了鉴定和定量:草地蜂蜜(MH,22个样品)、森林蜂蜜(FH,10)、阿拉伯树胶(AH,7)、栗子蜂蜜(CH,5)和石南蜂蜜(HH,5)。此外,还评估了这些蜂蜜类型的上清液和散装(非离心)溶液(b)中FC和总亲水性抗氧化得分(对两者的抗氧化活性:ROO·+OH·)之间的相关性。此外,还检测了相同蜂蜜样品中黄酮类化合物含量(FC)与先前报道的对过氧自由基和羟基自由基的抗氧化活性(AC(ROO·)和AC(OH·))之间的相关性。采用高效液相色谱-光电二极管阵列检测器(HPLC-DAD)和等度洗脱模式作为分析方法。采用固相萃取法提取黄酮类化合物。MH中三种黄酮类化合物(白杨素、柚皮素和木犀草素)的平均含量均高于AH(p<0.01),FH中柚皮素的平均含量在统计学上高于CH(p*<0.05)。我们观察到四种蜂蜜类型(FH,AH,HH,CH)的FC和AC(ROO·)之间存在高(正)线性相关性(R2=0.920)。如果我们将三种蜂蜜类型的FC和a C(ROO)s相关,则线性非常高(R2=0.968),并且FH、AH、CH的线性是完全的。相同蜜型的b中FC和AC(ROO·)的相关性相似,但较低。FC和AC(OH·)在五种或四种蜂蜜类型的s和b中均不存在相关性,但三种蜂蜜类型(FH、AH、CH)的FC与AC(OH.)s和AC(OH.)b均呈中等线性(R2=0.732和R2=0.696)。关键词:抗氧化活性,相关性,黄酮类化合物,蜂蜜,HPLC-DAD。
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引用次数: 0
Bagasse Yield and Quality Traits of Silage Made From Juice-Extracted Sweet Sorghum 甜高粱榨汁青贮的甘蔗渣产量及品质性状研究
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3017
C. Yucel, F. Bilgin, İlker İnal
This study was conducted to investigate possible use of juice-extracted sweet sorghum stalks (bagasse) as a quality roughage source through silage making. A total of 21 different sweet sorghum (Sorghum bicolor var. saccharatum (L.) Mohlenbr.) genotypes supplied from different sources were used as the primary material of the study. Field experiments were conducted in a randomized blocks design with 4 replications on the experimental fields of Eastern Mediterranean Agricultural Research Institute under 2nd crop conditions during the growing seasons of 2016 and 2017 years. Plants were harvested for bioethanol production at milk-dough stage of panicle grains. Plant leaves and panicles were stripped and remaining stalks were extracted through squeezing. Juice-extracted stalks (bagasse) were ensilaged for 60 days and quality traits were analyzed at the end of silage period. As the average of two years, bagasse yields varied between 42.6-113.9 t ha-1, silage DM yields varied between 11.6-40.0 t ha-1. In addition, crude protein (CP) content, acid detergent lignin (ADL), neutral detergent fiber (NDF) and acid detergent fiber (ADF) values varied between 29.79 - 50.84 g/kg DM, between 49.3 - 91.4 g/kg DM, between 525.1-694.8 g/kg DM and between 351.2-486.8 g/kg DM, respectively. It was concluded based on present findings that silages made from juice-extracted stalks of sweet sorghum grown under 2nd crop conditions of Cukurova region could be used as quality roughage source for livestock.Keywords: Bagasse yield; Genotype; Silage quality; Sweet sorghum
本研究旨在探讨甘蔗渣在青贮过程中作为优质粗料来源的可能性。共有21种不同的甜高粱(sorghum bicolor var. saccharatum, L.)从不同来源提供的Mohlenbr.)基因型作为研究的主要材料。田间试验采用随机区组设计,4个重复,于2016年和2017年第二季在东地中海农业研究所实验田进行。在穗粒的乳面团期收获植株用于生产生物乙醇。剥去植物叶片和圆锥花序,挤压提取剩余的茎。将榨汁秸秆(甘蔗渣)青贮60 d,并在青贮期末对品质性状进行分析。平均两年,甘蔗渣产量变化在42.6 ~ 113.9 t hm -1之间,青贮DM产量变化在11.6 ~ 40.0 t hm -1之间。粗蛋白质(CP)含量、酸性洗涤木质素(ADL)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)值分别在29.79 ~ 50.84 g/kg DM、49.3 ~ 91.4 g/kg DM、525.1 ~ 694.8 g/kg DM和351.2 ~ 486.8 g/kg DM之间变化。综上所述,库库罗娃地区二季条件下甜高粱榨汁秸秆青贮可作为家畜优质粗饲料来源。关键词:甘蔗渣产率;基因型;青贮饲料质量;甜高粱
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引用次数: 0
Phenolic screening and anticancer potential of various wild savory extracts 各种野生香料提取物的酚类筛选及其抗癌潜力
IF 1.1 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-11 DOI: 10.9755/ejfa.2023.v35.i4.3049
E. N. Şi̇mşek Sezer
Cancer is one of the leading causes of death in our age. In addition to the treatments used in cancer, plants are frequently used in complementary or alternative approaches. Plants of the genus Satureja are used world wide for various purposes, primarily as complementary therapies. For this purpose, in this study, the antioxidant potential and phenolic content of the Satureja cuneifolia plant, which is frequently consumed as a spice and tea among the public, and its cytotoxic and apoptosis-inducing effects on cancer cells were tried to be revealed. The cytotoxic effect of the extracts prepared with three different solvents (methanol, ethanol and water) was determined by the MTT test in colorectal cancer (DLD1) and promyelocytic leukaemia cell lines (HL60). Then the expression levels of five apoptotic gene regions (apaf-1, bax, bcl2, card4, casp3 and tp53) were evaluated with Real-Time PCR. The antioxidant potential was determined via the DPPH test, and HPLC was used to screen for phenolic substances. As a result, it was determined that the extracts have cytotoxic effects and have a variable but positive effect on pro-apoptotic gene expressions. When the antioxidant potential was evaluated, it was determined that the methanolic extract had a more radical scavenging effect (IC50: 105.02±0.034 µg/ml). In conclusion, the cytotoxic and apoptosis-inducing potentials of the extracts obtained from the S. cuneifolia plant were revealed for the first time upon DLD1 and HL60 cell lines in this study. This study is a pioneer for future studies in cancer therapy.Keywords: Apoptosis; qRT-PCR; Spice; Turkey; Wild savory
癌症是我们这个时代死亡的主要原因之一。除了用于癌症的治疗外,植物还经常以互补或替代的方式使用。Satureja属植物在世界各地被用于各种目的,主要是作为补充疗法。为此,在本研究中,试图揭示楔形萨图雷植物的抗氧化潜力和酚类含量,该植物在公众中经常被用作香料和茶,以及其对癌症细胞的细胞毒性和凋亡诱导作用。通过MTT试验测定了用三种不同溶剂(甲醇、乙醇和水)制备的提取物对大肠癌细胞株(DLD1)和早幼粒细胞白血病细胞株(HL60)的细胞毒性作用。然后用实时PCR检测5个凋亡基因区(apaf-1、bax、bcl2、card4、casp3和tp53)的表达水平。通过DPPH测试测定抗氧化潜力,并使用HPLC筛选酚类物质。结果,确定提取物具有细胞毒性作用,并且对促凋亡基因表达具有可变但积极的作用。当评估抗氧化潜力时,确定甲醇提取物具有更大的自由基清除作用(IC50:105.02±0.034µg/ml)。总之,在本研究中,首次揭示了从楔叶植物中获得的提取物对DLD1和HL60细胞系的细胞毒性和诱导细胞凋亡的潜力。这项研究是癌症治疗未来研究的先驱。关键词:细胞凋亡;qRT-PCR;香料土耳其野生咸味
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引用次数: 0
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Emirates Journal of Food and Agriculture
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