Rabeb Lassoued, M. Abderrabba, J. Mejri, Wafa Najar Benahmed, Aymen Benhmed, Mounir Ferhi
The present research work examined and compared the morphological, physicochemical and functional characteristics extracted from two oak varietiesgrowing inTunisia. Among the findings, it was observed that Quercus ilex's starch granules had the the hightest value of lipid and phosphor content. When observed under scanning electron microscopy, both Quercus ilex and Quercus coccifera starch granules exhibited various shapes such as ovoid, spherical, elliptical and irregular, with diameters ranging from 4 to 25 µm. As for the X-ray diffraction patterns, acorn starch from both varieties displayed an A-type pattern. Comparing the properties, Quercus coccifera acorn starch exhibited higher values for the light transmittance values, the solubility, the swelling power and the gelatinization temperatures than Quercus ilex acorn starch. However, the transition enthalpy of Quercus coccifera acorn starch was the lowest among the studied parameters. These results indicate that these properties are significantly (p<0.05) different depending on the oak variety. Thus, the starches of Quercus ilex and Quercus coccifera could play different roles in various industrial applications. Keywords: acorn, functional properties starch,Quercus,physicochemical properties
本研究工作考察并比较了从生长在突尼斯的两个橡树品种中提取的形态、理化和功能特征。研究结果表明,槲树淀粉颗粒中的脂质和磷含量最高。在扫描电子显微镜下观察,柞树和柞树淀粉颗粒呈现出各种形状,如卵圆形、球形、椭圆形和不规则形,直径在 4 至 25 微米之间。至于 X 射线衍射图样,两个品种的橡子淀粉都显示出 A 型图案。比较两种淀粉的特性,柞树橡子淀粉的透光率、溶解度、膨胀力和糊化温度均高于栎树橡子淀粉。然而,在所研究的参数中,柞树橡子淀粉的转化焓最低。这些结果表明,这些特性因橡树品种的不同而有显著差异(p<0.05)。因此,栎类和柞树的淀粉在各种工业应用中可以发挥不同的作用。 关键词:橡子;功能特性淀粉;柞树;理化特性
{"title":"Physicochemical, morphological and functional characteristics of starch isolated from Quercus ilex and Quercus coccifera","authors":"Rabeb Lassoued, M. Abderrabba, J. Mejri, Wafa Najar Benahmed, Aymen Benhmed, Mounir Ferhi","doi":"10.9755/ejfa.2023.3185","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3185","url":null,"abstract":"The present research work examined and compared the morphological, physicochemical and functional characteristics extracted from two oak varietiesgrowing inTunisia. Among the findings, it was observed that Quercus ilex's starch granules had the the hightest value of lipid and phosphor content. When observed under scanning electron microscopy, both Quercus ilex and Quercus coccifera starch granules exhibited various shapes such as ovoid, spherical, elliptical and irregular, with diameters ranging from 4 to 25 µm. As for the X-ray diffraction patterns, acorn starch from both varieties displayed an A-type pattern. Comparing the properties, Quercus coccifera acorn starch exhibited higher values for the light transmittance values, the solubility, the swelling power and the gelatinization temperatures than Quercus ilex acorn starch. However, the transition enthalpy of Quercus coccifera acorn starch was the lowest among the studied parameters. These results indicate that these properties are significantly (p<0.05) different depending on the oak variety. Thus, the starches of Quercus ilex and Quercus coccifera could play different roles in various industrial applications. Keywords: acorn, functional properties starch,Quercus,physicochemical properties","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md. Afjal Ahmad, Pravin Prakash, J. P. Srivastava, K. Dujeshwer, Anil K. Singh, H. S. Jatav
A laboratory experimentation was done in the Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University to evaluate elevated night temperature effect in wheat (Triticum aestivum L.) to see the response in some antioxidant and biochemical parameters in two wheat cultivars viz. HUW-234 (comparatively tolerant to heat stress) and sonalika (comparatively susceptible to heat stress). A significance difference was recorded in the parameters pertaining to antioxidant system includes catalase, SOD and APX activity were found to increase substantially when the plants kept under stress conditions (250C/ 240C day and night temperature) than the plants kept under controlled condition (250C/150C day and night temperature). The percent increase in the antioxidant enzyme activity was found to be more in the tolerant genotype (HUW-234) than in the susceptible genotype (Sonalika). There was 23.52 % increase was reported in the catalase activity by tolerant genotype when kept under stress conditions than those kept under control. Significant reductions were reported in most of the photosynthetic and membrane parameters viz., chlorophyll ‘a’, chlorophyll ‘b’ and membrane thermostability index. Similar percent reduction was reported with respect to biochemical parameters (total protein content, starch content and soluble sugar content) in the susceptible variety kept under high night temperature regime. Based on the pooled data of two-year experimentation it can be concluded that variety HUW-234 was found to be more tolerant than variety sonalika to HNT stress. Key Words: High night temperature, Wheat, Catalase activity, Anti-oxidant system.
{"title":"Physiological and Biochemical Effect of Elevated Night Temperature Stress on Bread Wheat (Triticum aestivum L.)","authors":"Md. Afjal Ahmad, Pravin Prakash, J. P. Srivastava, K. Dujeshwer, Anil K. Singh, H. S. Jatav","doi":"10.9755/ejfa.2023.3186","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3186","url":null,"abstract":"A laboratory experimentation was done in the Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University to evaluate elevated night temperature effect in wheat (Triticum aestivum L.) to see the response in some antioxidant and biochemical parameters in two wheat cultivars viz. HUW-234 (comparatively tolerant to heat stress) and sonalika (comparatively susceptible to heat stress). A significance difference was recorded in the parameters pertaining to antioxidant system includes catalase, SOD and APX activity were found to increase substantially when the plants kept under stress conditions (250C/ 240C day and night temperature) than the plants kept under controlled condition (250C/150C day and night temperature). The percent increase in the antioxidant enzyme activity was found to be more in the tolerant genotype (HUW-234) than in the susceptible genotype (Sonalika). There was 23.52 % increase was reported in the catalase activity by tolerant genotype when kept under stress conditions than those kept under control. Significant reductions were reported in most of the photosynthetic and membrane parameters viz., chlorophyll ‘a’, chlorophyll ‘b’ and membrane thermostability index. Similar percent reduction was reported with respect to biochemical parameters (total protein content, starch content and soluble sugar content) in the susceptible variety kept under high night temperature regime. Based on the pooled data of two-year experimentation it can be concluded that variety HUW-234 was found to be more tolerant than variety sonalika to HNT stress. Key Words: High night temperature, Wheat, Catalase activity, Anti-oxidant system.","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María E. Jimenez, Helen E. Berrus, Joselyn D. Segarra, Oscar L. Peralta, Mercy P. Pólit, Lisbeth C. Campuzano, Raisa A. Carpio, María P. Fondevila, María C. Villacres
Objective. To determine whether Lentinula edodes (L edodes) cultured on eucalyptus chips in the Ecuadorian highland generates antioxidant metabolites and anti-inflammatory effects. Methods. Total phenolic content was determined by the Folin-Ciocalteu reaction, the in vitro antioxidant activity of L edodes extracts was evaluated by the DPPH method and the in vivo anti-inflammatory activity was studied in mice. The chemical composition was studied by phytochemical screening and gas chromatography/mass spectrometry (GC/MS). Results. Total phenols were higher in extracts with increasingly high-water content. The antioxidant activity was robust and significantly strong in these extracts, suggesting the active metabolites are water-soluble. The anti-inflammation activity was significant in aqueous extracts only. Phytochemical screening indicated an overall similar composition to the literature reported earlier. GC/MS detected galactitol, trehalose, xylitol, phosphoric acid and octadecanoic acid among the most abundant metabolites. Conclusions. Cultivation on eucalyptus biomass at the Ecuadorian highlands retains the overall chemical composition, the phenolic content, antioxidant levels, and the in vivo anti-inflammatory activity of L edodes. The 11.3% content of trehalose observed is interesting for its capacity to control cellular stress damage. Key Words: inflammation, antioxidants, phenols, shiitake, Lentinula, phytotherapeutics
{"title":"Phenolic content and antioxidant activity in Lentinula edodes grown on eucalyptus biomass","authors":"María E. Jimenez, Helen E. Berrus, Joselyn D. Segarra, Oscar L. Peralta, Mercy P. Pólit, Lisbeth C. Campuzano, Raisa A. Carpio, María P. Fondevila, María C. Villacres","doi":"10.9755/ejfa.2023.3184","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3184","url":null,"abstract":"Objective. To determine whether Lentinula edodes (L edodes) cultured on eucalyptus chips in the Ecuadorian highland generates antioxidant metabolites and anti-inflammatory effects. Methods. Total phenolic content was determined by the Folin-Ciocalteu reaction, the in vitro antioxidant activity of L edodes extracts was evaluated by the DPPH method and the in vivo anti-inflammatory activity was studied in mice. The chemical composition was studied by phytochemical screening and gas chromatography/mass spectrometry (GC/MS). Results. Total phenols were higher in extracts with increasingly high-water content. The antioxidant activity was robust and significantly strong in these extracts, suggesting the active metabolites are water-soluble. The anti-inflammation activity was significant in aqueous extracts only. Phytochemical screening indicated an overall similar composition to the literature reported earlier. GC/MS detected galactitol, trehalose, xylitol, phosphoric acid and octadecanoic acid among the most abundant metabolites. Conclusions. Cultivation on eucalyptus biomass at the Ecuadorian highlands retains the overall chemical composition, the phenolic content, antioxidant levels, and the in vivo anti-inflammatory activity of L edodes. The 11.3% content of trehalose observed is interesting for its capacity to control cellular stress damage. Key Words: inflammation, antioxidants, phenols, shiitake, Lentinula, phytotherapeutics","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pickled Chinese cabbage traditional fermented vegetables is popular in China, especially the northeast, owing to their special flavors. Spontaneous fermented Chinese cabbage usually has rich and mellow flavor because of abundant microorganism in the fermentation broth. In our study, LAB were screened from spontaneous Chinese cabbage and identified including morphology, physiology and biochemistry, and molecular levels. 11 of these were used as a single inocula to ferment Chinese cabbage, and acid contents, soluble sugar contents, Vc contents, cellulose contents and nitrite contents of these pickled Chinese cabbage were measured. Overall, three superior strains mixture(X1, M2, and D2) were inoculated to improve the fermentation characteristics of pickled Chinese cabbage. Furthermore, pickled Chinese cabbage with the mixture had the highest acid and Vc contents, the lowest nitrite content, and medium fiber content. The sensory quality mixed inocula was also the best compared with products prepared with single strain and spontaneous fermentation. Keywords: Lactobacillus plantarum; Leuconostoc mesenteroides; pickled Chinese cabbage, mixed LAB
{"title":"Effects of adding isolated Lactobacillus plantarum, Leuconostoc mesenteroides, and their mixtures on the quality indices of pickled Chinese cabbage","authors":"Yan Liu, Meng Yang, Dalong Li, Yaowei Zhang","doi":"10.9755/ejfa.2023.3183","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3183","url":null,"abstract":"Pickled Chinese cabbage traditional fermented vegetables is popular in China, especially the northeast, owing to their special flavors. Spontaneous fermented Chinese cabbage usually has rich and mellow flavor because of abundant microorganism in the fermentation broth. In our study, LAB were screened from spontaneous Chinese cabbage and identified including morphology, physiology and biochemistry, and molecular levels. 11 of these were used as a single inocula to ferment Chinese cabbage, and acid contents, soluble sugar contents, Vc contents, cellulose contents and nitrite contents of these pickled Chinese cabbage were measured. Overall, three superior strains mixture(X1, M2, and D2) were inoculated to improve the fermentation characteristics of pickled Chinese cabbage. Furthermore, pickled Chinese cabbage with the mixture had the highest acid and Vc contents, the lowest nitrite content, and medium fiber content. The sensory quality mixed inocula was also the best compared with products prepared with single strain and spontaneous fermentation. Keywords: Lactobacillus plantarum; Leuconostoc mesenteroides; pickled Chinese cabbage, mixed LAB","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139183032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ina Ramírez Miranda, David Betancur Ancona, Yolanda Moguel Ordóñez
The Melipona beecheii honey has bioactive compounds with high antioxidant activity that influence its colour. This research evaluated the influence of phenolic compounds and flavonoids on the colour and antioxidant activity of M. beecheii honeys. Forty-three honey samples from different site located in the low deciduous forest of Yucatan, Mexico, were extracted during the harvest and post-harvest seasons (2020 – 2021). The Pfund method was used to measure colour, Folin-Ciocalteu method for phenols measurement, aluminum chloride method for flavonoids determination, and ABTS and DPPH IC50 assay to determine antioxidants. The predominant colour of the honey was extra light amber and light amber at both seasons. Phenols ranged from 780 to 1317 mg/kg GAE; flavonoids from 36 to 55 mg/kg CE and antioxidant were 675 to 1161 µmol/kg TEAC by the ABTS and 361.8 to 173.0 mg/mL by the DPPH IC50. Phenols and flavonoids were correlated with colour and radical scavenging activity, indicating the influence of these compounds with the darker colour and higher antioxidant activity of honey. The statistically significant difference (P<0.05) between seasons could be associated with the botanical origin with great diversity of melliferous flora present in the low deciduous forest of the Yucatan Peninsula. Keywords: Antioxidant; colour; flavonoids; honey; Melipona beecheii.
{"title":"Polyphenol galangin induces the ROS and ER stress-mediated intrinsic and extrinsic apoptotic pathways towards human colon carcinoma HCT-116 cells","authors":"Ina Ramírez Miranda, David Betancur Ancona, Yolanda Moguel Ordóñez","doi":"10.9755/ejfa.2023.3192","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3192","url":null,"abstract":"The Melipona beecheii honey has bioactive compounds with high antioxidant activity that influence its colour. This research evaluated the influence of phenolic compounds and flavonoids on the colour and antioxidant activity of M. beecheii honeys. Forty-three honey samples from different site located in the low deciduous forest of Yucatan, Mexico, were extracted during the harvest and post-harvest seasons (2020 – 2021). The Pfund method was used to measure colour, Folin-Ciocalteu method for phenols measurement, aluminum chloride method for flavonoids determination, and ABTS and DPPH IC50 assay to determine antioxidants. The predominant colour of the honey was extra light amber and light amber at both seasons. Phenols ranged from 780 to 1317 mg/kg GAE; flavonoids from 36 to 55 mg/kg CE and antioxidant were 675 to 1161 µmol/kg TEAC by the ABTS and 361.8 to 173.0 mg/mL by the DPPH IC50. Phenols and flavonoids were correlated with colour and radical scavenging activity, indicating the influence of these compounds with the darker colour and higher antioxidant activity of honey. The statistically significant difference (P<0.05) between seasons could be associated with the botanical origin with great diversity of melliferous flora present in the low deciduous forest of the Yucatan Peninsula. Keywords: Antioxidant; colour; flavonoids; honey; Melipona beecheii.","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Setiyoningrum, G. Priadi, F. Afiati, D. Desnilasari, R. Kumalasari, R. Ekafitri
Fermentation factors in the making of modified plantain flour must be optimized to obtain optimum conditions that produce the desired characteristics of flour/starch. In this research, we used mix-cultured lactic acid bacteria (LAB) and yeast for plantain fermentation. Response Surface Methodology (RSM) was used to explain the quantitative relationship between input and response variables. The design used is Combine D-optimal. The quantitative independent variables (X) selected were A = culture concentration (lower limit 2%; upper limit 6%) and B = fermentation time (lower limit 8 hours; upper limit 24 hours). Descriptive variables were C = type of plantain (C1 = “tanduk” plantain; C2 = “nangka” plantain) and D = type of culture (D1 = SC; D2 = LAB1 + LAB2; D3 = LAB1 + LAB3; D4 = LAB2 + LAB3; D5 = LAB1 + LAB1 + SC; D6 = LAB1 + LAB3 + SC; D7 = LAB2 + LAB3 + SC). Parameters of flour characteristic analyzed or response (Y) were carboxyl number (Y1), total acid (Y2), swelling number (Y3), pH (Y4), paste clarity (Y5), gelatinization properties: peak (Y6); trough 1 (Y7); breakdown (Y8); final viscosity (Y9); setback(Y10); peak time (Y11); pasting temperature (Y12), water content (Y13), color : *L (Y14); *a(Y15); *b (Y16) and c (Y17). The result showed that a combination of 6% culture concentration and 12.62 hours of fermentation time with a desirability value of 0.66 was the optimum condition with carboxyl value 0.15, total acid 0.47, swelling rate 13.49, peak time 5397.02, breakdown 2259.22, final viscosity 4125.22, setback 98.99, and dL 65.59. Keywords: modification flour, optimum condition, fermentation
{"title":"Optimizing the making of modified plantain flour by the mixed-culture of lactic acid bacteria and yeast","authors":"F. Setiyoningrum, G. Priadi, F. Afiati, D. Desnilasari, R. Kumalasari, R. Ekafitri","doi":"10.9755/ejfa.2023.3181","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3181","url":null,"abstract":"Fermentation factors in the making of modified plantain flour must be optimized to obtain optimum conditions that produce the desired characteristics of flour/starch. In this research, we used mix-cultured lactic acid bacteria (LAB) and yeast for plantain fermentation. Response Surface Methodology (RSM) was used to explain the quantitative relationship between input and response variables. The design used is Combine D-optimal. The quantitative independent variables (X) selected were A = culture concentration (lower limit 2%; upper limit 6%) and B = fermentation time (lower limit 8 hours; upper limit 24 hours). Descriptive variables were C = type of plantain (C1 = “tanduk” plantain; C2 = “nangka” plantain) and D = type of culture (D1 = SC; D2 = LAB1 + LAB2; D3 = LAB1 + LAB3; D4 = LAB2 + LAB3; D5 = LAB1 + LAB1 + SC; D6 = LAB1 + LAB3 + SC; D7 = LAB2 + LAB3 + SC). Parameters of flour characteristic analyzed or response (Y) were carboxyl number (Y1), total acid (Y2), swelling number (Y3), pH (Y4), paste clarity (Y5), gelatinization properties: peak (Y6); trough 1 (Y7); breakdown (Y8); final viscosity (Y9); setback(Y10); peak time (Y11); pasting temperature (Y12), water content (Y13), color : *L (Y14); *a(Y15); *b (Y16) and c (Y17). The result showed that a combination of 6% culture concentration and 12.62 hours of fermentation time with a desirability value of 0.66 was the optimum condition with carboxyl value 0.15, total acid 0.47, swelling rate 13.49, peak time 5397.02, breakdown 2259.22, final viscosity 4125.22, setback 98.99, and dL 65.59. Keywords: modification flour, optimum condition, fermentation","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139183128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saleem Kaseemsaheb Nadaf, Jamal N. Al-Sabahi, A. H. Al Lawati, Almandhar Almamari, Fatima A. Al-Kindi, Abdulaziz Al Maawali, H. Al-Ruqaishi, Ahmed S. Al-Ghafri, Amina Al-Farsi, Nadiya Abubakar Al Saady
Plants have been used directly or indirectly as medicines for over 5000 years as a source of antibiotics, antineoplastics, analgesics, and cardio-protective, among others. Approximately 70–90% of the population in developing countries continue to use ancient medicines based on plant extracts for treatment. Recently, the isolation and identification of biologically active compounds and molecules from nature have led to the discovery of new therapeutics, prompting the improvement of the health and pharmaceutical sectors. Phytochemicals revolve around the pharmaceutical industry's research and development (R&D) sector as a source of new molecules leading to the development of new novel drugs. Given the above, the present investigation addresses the differential nature of wild lavender (Lavandula subnuda Benth) plants of diverse locations in Oman with respect to their chemical composition of the essential oil in addition to morphological characters and chlorophyll contents of leaves to explore the possibility of isolating its prime chemical compounds on a commercial scale in perfumery industry. There are no previous studies so far who have reported on essential oil recovery and chemical composition exclusively in respect of Lavandula subnuda. Composite samples of ten randomly selected plants were collected from wadi habitats of four diversified locations. Edaphic features of managed sites were recorded, and their soil chemical contents were determined following instructions using S1 Titan/Tracer 5/CTX equipment of Bruker developed based on energy dispersive X-ray fluorescence (EDXRF). Morphological traits were measured using a ruler, and chlorophyll contents were recorded using the atLEAF CHL Plus chlorophyll meter as atLEAF values. The essential oil was extracted using ETHO X's advanced microwave extraction system and analyzed for chemical compounds using GC-MS analysis on Shimadzu GC-2010 Plus gas chromatograph. The experimental data were analyzed statistically, wherever required, by applying basic statistics of the EXCEL -16 version. The results indicated that in general, the Lavandula subnuda plants of locations of high altitudes had higher expressivity in all the morphological traits and chlorophyll contents than those of low altitudes. The essential oil recovery, however, was found to be higher from the plant samples of lower altitudes (0.70 to 0.79% (w/w)) than from those of higher mountains/ altitudes (0.15 to 0.18% (w/w)). The pattern of values of commonly occurring chemical contents of essential oils was different. Each essential oil's top ten chemical compounds contributed about 80% of the total in four locations. Of the ten top chemical compounds, eight compounds, namely D-Germacrene (42.67%) from Wadi Al-Khod, Estragole (32%) and Linalool (23.89%) from Wadi Halban), trans-Borneol (23.46%) and 4-Terpineol (18.73%) from Wadi Najd Al-Waqba and Kessane (18.60%), beta-cis-Caryophyllene (13.68%) and beta-Elemene (10.618%) from Wadi Al-Hayul, w
{"title":"Differential Expression of Oman’s Wild Lavender, Lavandula subnuda for Chemical Composition towards Medicinal and Aromatic Application","authors":"Saleem Kaseemsaheb Nadaf, Jamal N. Al-Sabahi, A. H. Al Lawati, Almandhar Almamari, Fatima A. Al-Kindi, Abdulaziz Al Maawali, H. Al-Ruqaishi, Ahmed S. Al-Ghafri, Amina Al-Farsi, Nadiya Abubakar Al Saady","doi":"10.9755/ejfa.2023.3140","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3140","url":null,"abstract":"Plants have been used directly or indirectly as medicines for over 5000 years as a source of antibiotics, antineoplastics, analgesics, and cardio-protective, among others. Approximately 70–90% of the population in developing countries continue to use ancient medicines based on plant extracts for treatment. Recently, the isolation and identification of biologically active compounds and molecules from nature have led to the discovery of new therapeutics, prompting the improvement of the health and pharmaceutical sectors. Phytochemicals revolve around the pharmaceutical industry's research and development (R&D) sector as a source of new molecules leading to the development of new novel drugs. Given the above, the present investigation addresses the differential nature of wild lavender (Lavandula subnuda Benth) plants of diverse locations in Oman with respect to their chemical composition of the essential oil in addition to morphological characters and chlorophyll contents of leaves to explore the possibility of isolating its prime chemical compounds on a commercial scale in perfumery industry. There are no previous studies so far who have reported on essential oil recovery and chemical composition exclusively in respect of Lavandula subnuda. Composite samples of ten randomly selected plants were collected from wadi habitats of four diversified locations. Edaphic features of managed sites were recorded, and their soil chemical contents were determined following instructions using S1 Titan/Tracer 5/CTX equipment of Bruker developed based on energy dispersive X-ray fluorescence (EDXRF). Morphological traits were measured using a ruler, and chlorophyll contents were recorded using the atLEAF CHL Plus chlorophyll meter as atLEAF values. The essential oil was extracted using ETHO X's advanced microwave extraction system and analyzed for chemical compounds using GC-MS analysis on Shimadzu GC-2010 Plus gas chromatograph. The experimental data were analyzed statistically, wherever required, by applying basic statistics of the EXCEL -16 version. The results indicated that in general, the Lavandula subnuda plants of locations of high altitudes had higher expressivity in all the morphological traits and chlorophyll contents than those of low altitudes. The essential oil recovery, however, was found to be higher from the plant samples of lower altitudes (0.70 to 0.79% (w/w)) than from those of higher mountains/ altitudes (0.15 to 0.18% (w/w)). The pattern of values of commonly occurring chemical contents of essential oils was different. Each essential oil's top ten chemical compounds contributed about 80% of the total in four locations. Of the ten top chemical compounds, eight compounds, namely D-Germacrene (42.67%) from Wadi Al-Khod, Estragole (32%) and Linalool (23.89%) from Wadi Halban), trans-Borneol (23.46%) and 4-Terpineol (18.73%) from Wadi Najd Al-Waqba and Kessane (18.60%), beta-cis-Caryophyllene (13.68%) and beta-Elemene (10.618%) from Wadi Al-Hayul, w","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139269477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maxim S. Oshchepkov, A. Kalistratova, Leonid V. Kovalenko, Мaria S. Ivanova, M. Tsvetikova, N. Bystrova, Konstantin A. Kochetkov
The synthetic cytokinins belonging to the class of bifunctional carbamates and oxamates were tested for a biological activity in a wide concentration range of 10-2 M − 10-6 M along with chlorocholine chloride taken as reference. The compounds have a pronounced growth-regulatory activity for wheat seeds (Triticum aestivum L) at the optimal concentration of 10-5 M, at which the germination potential Gp and the germination rate Gr of seeds, as well as resistance to water stress, determined by RWC values reach their maximum values. It was found that the technique of spraying wheat seeds with solutions of the compounds used is more preferable than soaking. Key words: wheat, plant growth regulators, carbamates, oxamates, water stress
对属于双功能氨基甲酸酯类和草氨酸类的合成细胞分裂素进行了生物活性测试,测试浓度范围为 10-2 M - 10-6 M,并以氯化氯胆碱作为参照物。在 10-5 M 的最佳浓度下,这些化合物对小麦种子(Triticum aestivum L)具有明显的生长调节活性,在此浓度下,种子的发芽势 Gp 和发芽率 Gr 以及对水分胁迫的抗性(由 RWC 值决定)均达到最大值。研究发现,用所使用化合物的溶液喷洒小麦种子的技术比浸泡更为可取。 关键词:小麦、植物生长调节剂、氨基甲酸酯、草铵膦、水分胁迫
{"title":"Evaluation of potential and rate of the germination of wheat seeds (Triticum aestivum L) treated with bifunctional growth regulators under water stress","authors":"Maxim S. Oshchepkov, A. Kalistratova, Leonid V. Kovalenko, Мaria S. Ivanova, M. Tsvetikova, N. Bystrova, Konstantin A. Kochetkov","doi":"10.9755/ejfa.2023.3177","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3177","url":null,"abstract":"The synthetic cytokinins belonging to the class of bifunctional carbamates and oxamates were tested for a biological activity in a wide concentration range of 10-2 M − 10-6 M along with chlorocholine chloride taken as reference. The compounds have a pronounced growth-regulatory activity for wheat seeds (Triticum aestivum L) at the optimal concentration of 10-5 M, at which the germination potential Gp and the germination rate Gr of seeds, as well as resistance to water stress, determined by RWC values reach their maximum values. It was found that the technique of spraying wheat seeds with solutions of the compounds used is more preferable than soaking. Key words: wheat, plant growth regulators, carbamates, oxamates, water stress","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139278220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objectives of this study were to optimize the medium and culture conditions using a strong γ-aminobutyric acid (GABA) producer as a reference lactic acid bacterial strain, to screen and identify GABA-producing lactic acid bacterial isolates from kimchi, and to determine their extracellular GABA-producing abilities. Thin-layer chromatography was used to screen GABA-producing bacterial isolates and high-performance liquid chromatography was used to evaluate the bacterial GABA production abilities. Species-specific polymerase chain reaction analyses were used to identify GABA-producing bacterial isolates. The optimal medium and culture conditions were found to be the modified Man–Rogosa–Sharpe (MRS) broth (with an initial pH of 6.5) containing 4% sucrose, 5% glutamate, and 1% yeast extract at 37°C for 5 days. After incubation under the optimized culture conditions, 217 kimchi bacterial isolates were screened to evaluate their respective GABA-producing abilities. Screening the 217 kimchi bacterial isolates identified 24 GABA-producing lactic acid bacterial isolates (11%): Lactobacillus plantarum (17), Lactobacillus brevis (six), and Leuconostoc mesenteroides (one), indicating that only a small proportion of the strains produce GABA in the culture broth. The extracellular GABA-producing abilities of the bacterial strains identified in this study varied even within the same species, ranging from 5.8 to 101.7 mM among the 17 GABA-producing L. plantarum isolates and from 8.5 to 88.6 mM among the six GABA-producing L. brevis isolates. In summary, three species of the 24 kimchi GABA-producing bacterial isolates were identified, including one rare species (L. mesenteroides) and the two most dominant species (L. brevis and L. plantarum). Keywords: Lactobacillus brevis; Lactobacillus plantarum; Leuconostoc mesenteroides; Optimization; γ-aminobutyric acid (GABA)
{"title":"Optimization of culture conditions for and assessment of kimchi-originated lactic acid bacterial isolates toward their extracellular GABA-producing ability","authors":"Jin-Sung Lee, Keun-Sung Kim","doi":"10.9755/ejfa.2023.3158","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3158","url":null,"abstract":"The objectives of this study were to optimize the medium and culture conditions using a strong γ-aminobutyric acid (GABA) producer as a reference lactic acid bacterial strain, to screen and identify GABA-producing lactic acid bacterial isolates from kimchi, and to determine their extracellular GABA-producing abilities. Thin-layer chromatography was used to screen GABA-producing bacterial isolates and high-performance liquid chromatography was used to evaluate the bacterial GABA production abilities. Species-specific polymerase chain reaction analyses were used to identify GABA-producing bacterial isolates. The optimal medium and culture conditions were found to be the modified Man–Rogosa–Sharpe (MRS) broth (with an initial pH of 6.5) containing 4% sucrose, 5% glutamate, and 1% yeast extract at 37°C for 5 days. After incubation under the optimized culture conditions, 217 kimchi bacterial isolates were screened to evaluate their respective GABA-producing abilities. Screening the 217 kimchi bacterial isolates identified 24 GABA-producing lactic acid bacterial isolates (11%): Lactobacillus plantarum (17), Lactobacillus brevis (six), and Leuconostoc mesenteroides (one), indicating that only a small proportion of the strains produce GABA in the culture broth. The extracellular GABA-producing abilities of the bacterial strains identified in this study varied even within the same species, ranging from 5.8 to 101.7 mM among the 17 GABA-producing L. plantarum isolates and from 8.5 to 88.6 mM among the six GABA-producing L. brevis isolates. In summary, three species of the 24 kimchi GABA-producing bacterial isolates were identified, including one rare species (L. mesenteroides) and the two most dominant species (L. brevis and L. plantarum). Keywords: Lactobacillus brevis; Lactobacillus plantarum; Leuconostoc mesenteroides; Optimization; γ-aminobutyric acid (GABA)","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alfredo Olarte Paredes, Adaia Ramírez Contreras, Areli Salgado Delgado, Edgar García Hernández, René Salgado Delgado, T. Lara, Juan Bosco Hernández Zaragoza
Packaging is the main source of polluting plastic waste. Out of the total volume of plastic waste worldwide, the majority corresponds to food packaging. Biodegradable films have been used in a large number of applications across industrial sectors due to their property versatility and environmental factors. There is a growing interest in the search for packaging materials from renewable sources that prove to be functional for food preservation. Among the polymers used, starch has garnered the most attention due to its abundance in nature and biodegradable quality; it is also renewable and low-cost. A methodology was established to increase the shelf life of perishable fruits (blackberry) from 7 to 14 days by adding starch, chitosan, and roselle extract. These biopolymers synergistically contribute by providing support, antibacterial, and antioxidant properties, respectively. The use of chitosan and roselle extract were key to the achievement of this work’s goals, as proven by FT-IR, TGA, SEM, contact angle, and microbiological analyses. The present work focuses on the use of three elements that have not been studied together in biofilm design to preserve fruits.
{"title":"Obtention and characterization of chitosan/starch/roselle films for the preservation of perishable fruits","authors":"Alfredo Olarte Paredes, Adaia Ramírez Contreras, Areli Salgado Delgado, Edgar García Hernández, René Salgado Delgado, T. Lara, Juan Bosco Hernández Zaragoza","doi":"10.9755/ejfa.2023.3157","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3157","url":null,"abstract":"Packaging is the main source of polluting plastic waste. Out of the total volume of plastic waste worldwide, the majority corresponds to food packaging. Biodegradable films have been used in a large number of applications across industrial sectors due to their property versatility and environmental factors. There is a growing interest in the search for packaging materials from renewable sources that prove to be functional for food preservation. Among the polymers used, starch has garnered the most attention due to its abundance in nature and biodegradable quality; it is also renewable and low-cost. A methodology was established to increase the shelf life of perishable fruits (blackberry) from 7 to 14 days by adding starch, chitosan, and roselle extract. These biopolymers synergistically contribute by providing support, antibacterial, and antioxidant properties, respectively. The use of chitosan and roselle extract were key to the achievement of this work’s goals, as proven by FT-IR, TGA, SEM, contact angle, and microbiological analyses. The present work focuses on the use of three elements that have not been studied together in biofilm design to preserve fruits.","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}