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RSM-optimization of microwave-assisted extraction of R. laevigata polysaccharides with bioactivities RSM 优化微波辅助提取具有生物活性的 R. laevigata 多糖的方法
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-12 DOI: 10.9755/ejfa.2023.3159
Pinyi Gao, Xin Shen, Yongdan Guo, Mei Jin, Dandan Yue, Danqi Li, Xuegui Liu, Changfeng Liu
Rosa laevigata Michx. is an edible traditional Chinese medicinal herb that possesses several health benefits. This study optimized the microwave-assisted extraction conditions of polysaccharides from Rosa laevigata fruits and investigated the antimicrobial potency and the antioxidant activity of Rosa laevigata polysaccharides. The microwave power, extraction time, the number of extraction, and the solvent-to-solid ratio were established using a three-factor, three-level Box-Behnken Design (BBD) and Response Surface Methodology (RSM) using screening experiments. Maximum polysaccharides yields were obtained when extraction was done for 18 min at a microwave power of 516 W and using a liquid-solid ratio of 54 mL/g. The polysaccharides yield was 20.37 % under the optimized conditions (predictive value for 20.40 %). This study also determined the antioxidant activity and the antibacterial potency of Rosa laevigata fruits to enhance their functional characterization and medicinal use. The antioxidant experiment showed that when the concentration was 5.0 mg/mL, polysaccharides had the strongest scavenging ability to DPPH free radicals and ABTS free radicals, and the scavenging rate was 83.06 % and 99.38 % respectively. Furthermore, the antibacterial experiment showed that the polysaccharides exhibited prominent inhibition activities, the MIC against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) at 3.13 mg/mL and 1.56 mg/mL, respectively. Keywords: Rosa laevigata Michx.; polysaccharides; microwave-assisted extraction; antibacterial activity; antioxidant activity
蔷薇是一种可食用的传统中药材,具有多种保健功效。本研究优化了微波辅助提取蔷薇果多糖的条件,并考察了蔷薇果多糖的抗菌效力和抗氧化活性。通过筛选实验,采用三因素三水平箱式贝肯设计(BBD)和响应面方法(RSM)确定了微波功率、萃取时间、萃取次数和溶剂与固体的比例。在微波功率为 516 W、液固比为 54 mL/g 的条件下萃取 18 分钟,多糖产量最大。在优化条件下,多糖产量为 20.37%(预测值为 20.40%)。本研究还测定了蔷薇果的抗氧化活性和抗菌效力,以提高其功能特性和药用价值。抗氧化实验表明,当浓度为 5.0 mg/mL 时,多糖对 DPPH 自由基和 ABTS 自由基的清除能力最强,清除率分别为 83.06 % 和 99.38 %。此外,抗菌实验表明,多糖具有显著的抑菌活性,对金黄色葡萄球菌(S. aureus)和大肠杆菌(E. coli)的抑菌浓度分别为3.13 mg/mL和1.56 mg/mL。 关键词:蔷薇蔷薇多糖;微波辅助萃取;抗菌活性;抗氧化活性
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引用次数: 0
A study of the effects of green stem syndrome on some quality parameters in soybean and the possibility of early detection 青茎综合征对大豆品质参数的影响及早期检测的可行性研究
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-12 DOI: 10.9755/ejfa.2023.3161
Yasemin Vurarak, Pinar Cubukcu, Ahmet Korhan Sahar, Celile Aylin Oluk
Green stem syndrome is one of the major problems encountered in soybean production in the world because it makes harvesting with a combine harvester difficult. Although the prevalence of the green stem syndrome Turkey is unknown, in recent years it has started to be observed frequently. Leaf color characters in the growing stages of some soybean varieties have been determined according to varieties in this study. Color changes in the leaves from V3 to R8 phase were monitored using L *, a *, b * color scale. Possibility of detecting changes in leaf color before the R8 stage was studied. Some quality parameters have been evaluated in seed samples obtained from plants with and without symptoms in the R8 stage. It was determined that the germination rate of the seeds obtained from the plants with the syndrome decreased by 61.4% on average compared to those from healthy plants. Furthermore, compared to non-symptomatic seeds, symptomatic seeds were larger, had a lower fat ratio, lower palmitic and linoleic fatty acid values, and higher oleic fatty acid values. At this study was determined that the most significant difference was manifested in terms of stem moisture values during germination and harvesting. In addition, detection of green stem syndrome can be used b* color value as a marker. The hypothesis of the study is that the syndrome can be diagnosed at early stage by following color values in the soybean leaves. In the future studies the color of the leaf can also be a parameter available for the machine learning models. Keywords: Harvest stage, Glycine max (L.), green stem syndrome, leaf color, machine learning
绿茎综合征是世界大豆生产中遇到的主要问题之一,因为它使联合收割机的收获变得困难。虽然土耳其绿茎综合征的患病率尚不清楚,但近年来已开始频繁观察到。本研究根据品种测定了部分大豆品种生长阶段的叶色特征。采用L *、a *、b *色阶对V3 ~ R8期叶片的颜色变化进行监测。研究了在R8期前检测叶片颜色变化的可能性。对R8期有症状和无症状植物的种子样品进行了一些质量参数评价。结果表明,与健康植株相比,该综合征植株的种子发芽率平均下降61.4%。此外,与无症状种子相比,有症状种子体积较大,脂肪比较低,棕榈酸和亚油酸值较低,油酸值较高。在本研究中,最显著的差异表现在发芽和收获期间的茎水分值。另外,检测青茎综合征可用b*色值作为标记。本研究的假设是,通过观察大豆叶片的颜色值,可以在早期诊断出该综合征。在未来的研究中,叶子的颜色也可以作为机器学习模型的一个参数。 关键词:收获期,甘氨酸max (L.),绿茎综合征,叶色,机器学习
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 Keywords: Harvest stage, Glycine max (L.), green stem syndrome, leaf color, machine learning
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引用次数: 0
Amino acid and biochemical composition of Banded Carpet Shell (Polititapes rhomboides, Pennant, 1777), collected from the Çardak Lagoon, Turkey 土耳其Çardak泻湖带状地毯壳(Polititapes rhomboides, Pennant, 1777)的氨基酸和生化组成
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-12 DOI: 10.9755/ejfa.2023.3156
Pervin Vural
Between January and December 2019, banded carpet shell (Polititapes rhomboides, Pennant 1777) were gathered from Çardak Lagoon (Çanakkale Strait). The length value was found to range from 32.92 to 36.37 mm, weight between 7.90 and 11.75 g, condition index from 4.44 to 8.70, and meat yield was found to be ranging from 11.95% to 24.95%. Negative allometry was determined in the W/L relationship of P. rhomboides. The main meat component of P. rhomboides was moisture (54.70-65.57%) and protein (52.89-62.10%) with the other components being carbohydrate (10.95-21.30%), lipid (12.79-17.18%) and ash (7.69-19.93%). The correlation between moisture and carbohydrate was positive, whereas the correlation between moisture and protein was negative (p<0.05). The present study not only informs us on composition but also recommends consumption of the species since it is easier to market and consume. Arginine amino acid was found to be the highest in quantity, which was followed by glutamic acid, serine, methionine, alanine, and aspartic acid. Keywords: Length-weight relationship, Meat yield, Protein, Lipid, Çanakkale Strait.
2019年1月至12月期间,从Çardak泻湖(Çanakkale海峡)收集了带状地毯贝壳(Polititapes rhomboides, Pennant 1777)。体长值为32.92 ~ 36.37 mm,体重值为7.90 ~ 11.75 g,状态指数为4.44 ~ 8.70,产肉率为11.95% ~ 24.95%。菱形蕨的W/L呈负异速生长关系。肉质成分以水分(54.70 ~ 65.57%)和蛋白质(52.89 ~ 62.10%)为主,其余分别为碳水化合物(10.95 ~ 21.30%)、脂肪(12.79 ~ 17.18%)和灰分(7.69 ~ 19.93%)。水分与碳水化合物呈显著正相关,与蛋白质呈显著负相关(p < 0.05)。目前的研究不仅告诉我们成分,而且还建议消费该物种,因为它更容易销售和消费。精氨酸含量最高,其次是谷氨酸、丝氨酸、蛋氨酸、丙氨酸和天冬氨酸。& # x0D;关键词:长权关系,肉产量,蛋白质,脂肪,Çanakkale海峡。
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引用次数: 0
Phenological, morphological and genetic characterization of local grapevine (Vitis labrusca L.) genotypes grown in the Black Sea Region in Northern Turkey 土耳其北部黑海地区本地葡萄(Vitis labrusca L.)基因型物候、形态和遗传特征
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-12 DOI: 10.9755/ejfa.2023.3160
Birsen Çakır Aydemir, Kezban Yazıcı, Burcu Göksu
Vitis labrusca has become widely naturalized in the Black Sea region of Northern Turkey. The aim of this work was to evaluate the morphological, phenological, and genetic characteristics of V. labrusca accessions grown in the Black Sea region during the last three growing seasons. Local genotypes were described according to the Organisation Internationale de la Vigne et du Vin (OIV) ampelographic descriptor, including shoot length (cm), mature leaf size, bunch length and width, berry length and width, berry weight, number of berries, Total Soluble Solids (TTS) and titratable acidity. Additionally, phenological data, including bud burst, flowering, veraison and harvest date, were recorded. The accessions were characterized at the molecular level, and genetic relationships were assessed by means of Inter Simple Sequence Repeat (ISSR) markers. Using 6 ISSR primers, seventy-seven bands were obtained, of which 69 were polymorphic with a mean polymorphic rate of 88.68%. These ISSR primers produced polymorphism information content (PIC) values ranging from 0.48 to 0.5. The genetic similarity ranged from 0.08 to 0.83 among the genotypes. According to the dendrogram based on the ISSR analysis, Accessions 8 and 5 were genetically related, with a coefficient of similarity of 0.83, while Accession 3 was the most distantly related genotype, with a coefficient of similarity of 0.08. These results demonstrated that ISSR markers can be used for genetic diversity analysis among V. labrusca genotypes. Our results also described characteristics of new V. labrusca genotypes that could be valuable for future Marker-Assisted Selection (MAS) and grapevine breeding. Key words: Diversity, foxy species, Inter-Simple Sequence Repeat markers (ISSRs), Breeding, polymorphism
在土耳其北部的黑海地区,拉布鲁斯卡葡萄已被广泛归化。本研究的目的是评价黑海地区近三个生长季节生长的紫花苜蓿的形态、物候和遗传特征。根据国际葡萄和葡萄组织(OIV)的地形描述描述,描述了当地的基因型,包括茎长(cm)、成熟叶大小、束长和宽度、浆果长和宽度、浆果重量、浆果数量、总可溶性固形物(TTS)和可滴定酸度。此外,还记录了物候数据,包括发芽、开花、品种和收获日期。利用ISSR (Inter Simple Sequence Repeat)标记对所选材料进行了分子水平的鉴定和亲缘关系分析。6条ISSR引物共获得77条条带,其中多态性69条,平均多态性率为88.68%。这些ISSR引物产生的多态性信息含量(PIC)值在0.48 ~ 0.5之间。基因型间遗传相似性为0.08 ~ 0.83。基于ISSR分析的树状图显示,8号和5号基因型亲缘关系较好,相似系数为0.83,3号基因型亲缘关系最远,相似系数为0.08。结果表明,ISSR标记可用于油菜基因型间的遗传多样性分析。我们的研究结果还描述了新的labrusca基因型的特征,这些特征可能对未来的标记辅助选择(MAS)和葡萄育种有价值。关键词:多样性,狐种,ISSRs,育种,多态性
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引用次数: 0
Preliminary study of Nicuatole, a traditional endemic food based on Zea mays from the Central Valleys Region, Oaxaca, Mexico 墨西哥瓦哈卡中央山谷地区以玉米为原料的传统地方食物Nicuatole的初步研究
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-11 DOI: 10.9755/ejfa.2023.3155
Morales-Sánchez José Luis, Hernández-Bautista Emilio, Sánchez-Medina Marco Antonio, Pérez-Santiago Alma Dolores, Matías-Pérez Diana, García-Montalvo Iván Antonio
The present investigation evaluated the pre-Hispanic food called "nicuatole" based on corn, from the Central Valleys region of the state of Oaxaca, Mexico. The objective of the present work was to present preliminary results of the physicochemical and proximate properties of the pre-Hispanic food locally known as "nicuatole". The sample was prepared based on the traditional endemic recipe of the population of San Agustín Yatareni, Oaxaca, Mexico. To determine the physicochemical composition, samples of 10 g and 3 g were used for the tests (pH and density) and nutritional tests (moisture, proteins, lipids, minerals, and carbohydrates) based on the methods described by the Association of Official Analytical Chemistry (AOAC). As results, it was obtained that it has a pH of 6.18 and a density of 1.0812 g*mL-1. The main results of the proximal analysis and chemical composition of "nicuatole" were 2.1325% protein; 1.54% ash; 66.3738% humidity content; 0.7563% lipids; 9.6271% carbohydrates; 13.1731% fiber. The pre-Hispanic food known as "nicuatole" can be considered a healthy alternative for the Mexican population. Keywords: Pre-hispanic food; Nicuatole; Zea mays; Mexico; Dessert
目前的调查评估了一种前西班牙食物,名为“nicuatole”,以玉米为基础,来自墨西哥瓦哈卡州的中央山谷地区。本工作的目的是提出前西班牙食物的物理化学和近似性质的初步结果,当地称为“nicuatole”。该样品是根据墨西哥瓦哈卡州San Agustín Yatareni人口的传统地方性配方制备的。为了确定理化成分,根据官方分析化学协会(AOAC)描述的方法,使用10 g和3 g样品进行测试(pH值和密度)和营养测试(水分、蛋白质、脂质、矿物质和碳水化合物)。结果表明,其pH为6.18,密度为1.0812 g*mL-1。“nicuatole”的主要近端分析结果和化学成分为2.1325%的蛋白质;1.54%灰;66.3738%湿度含量;0.7563%的脂质;碳水化合物9.6271%;13.1731%的纤维。被称为“nicuatole”的前西班牙食物可以被认为是墨西哥人的健康替代品。关键词:前西班牙食物;Nicuatole;玉米;墨西哥;甜点# x0D;
{"title":"Preliminary study of Nicuatole, a traditional endemic food based on Zea mays from the Central Valleys Region, Oaxaca, Mexico","authors":"Morales-Sánchez José Luis, Hernández-Bautista Emilio, Sánchez-Medina Marco Antonio, Pérez-Santiago Alma Dolores, Matías-Pérez Diana, García-Montalvo Iván Antonio","doi":"10.9755/ejfa.2023.3155","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3155","url":null,"abstract":"The present investigation evaluated the pre-Hispanic food called \"nicuatole\" based on corn, from the Central Valleys region of the state of Oaxaca, Mexico. The objective of the present work was to present preliminary results of the physicochemical and proximate properties of the pre-Hispanic food locally known as \"nicuatole\". The sample was prepared based on the traditional endemic recipe of the population of San Agustín Yatareni, Oaxaca, Mexico. To determine the physicochemical composition, samples of 10 g and 3 g were used for the tests (pH and density) and nutritional tests (moisture, proteins, lipids, minerals, and carbohydrates) based on the methods described by the Association of Official Analytical Chemistry (AOAC). As results, it was obtained that it has a pH of 6.18 and a density of 1.0812 g*mL-1. The main results of the proximal analysis and chemical composition of \"nicuatole\" were 2.1325% protein; 1.54% ash; 66.3738% humidity content; 0.7563% lipids; 9.6271% carbohydrates; 13.1731% fiber. The pre-Hispanic food known as \"nicuatole\" can be considered a healthy alternative for the Mexican population.&#x0D; Keywords: Pre-hispanic food; Nicuatole; Zea mays; Mexico; Dessert&#x0D;","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136025469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The selection of intra-inter specific Cucurbita rootstocks for grafted melon seedlings 瓜种内种间嫁接砧木的选择
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-10 DOI: 10.9755/ejfa.2023.3152
Dilek KANDEMİR
This study was conducted to select promising Turkish local Cucurbita rootstocks among intraspecific and interspecific hybrids for grafted melon seedling based on hybrid success rate, hypocotyl traits and graft compatibility. Rooting potentials, root system architecture and plant growth parameters of the rootstocks grafted with melon were also evaluated. Two lines of C. maxima (male parent) and eleven lines of C. moschata (female parent) at S5 generation were used. In addition, six winter squash lines were used as male parent and six pumpkin lines were used as female parent in intraspecific hybridization program. Intraspecific hybrid combinations had quite high number of seeds per fruit as compared to interspecific hybrid combinations. The highest number of seeds per fruit was obtained from HMO2 × OMO2 (359) and OMO5 × HMO8 (314) hybrid combinations. Hypocotyl lengths of all hybrid cucurbit rootstock combinations ranged from 32.96 mm (RS17) to 73.35 mm (RS8) and hypocotyl thicknesses ranged from 2.71 mm (RS2) to 3.55 mm (RS8). Graft success rates varied between 96.6 – 100% in winter squash and pumpkin intraspecific hybrids and between 87.1 – 100% in interspecific hybrids. Significant differences were seen in root architecture parameters (root length, root volume, mean root diameter, root surface area and root dry matter weight) of intraspecific and interspecies hybrid Cucurbita lines. The findings of the study indicated that the intraspecific and interspecific local cucurbit rootstock candidates developed within the rootstock breeding program showed promise as viable options for commercial use as rootstocks for grafted melon seedlings. Research about the effects of selected intraspecific and interspecific hybrid Cucurbita rootstock candidates on earliness, fruit quality and yield parameters of grafted melon will continue. Keywords: Cucurbita maxima; Cucurbita moschata; Rootstock breeding; Root system architecture; Melon
本研究根据杂交成功率、下胚轴性状和嫁接相容性,从种内和种间杂交中选择有前途的土耳其地方瓜砧木嫁接瓜苗。并对嫁接甜瓜的砧木的生根势、根系构型和植株生长参数进行了评价。在S5代选用了2个大花花(父本)系和11个花花花(母本)系。另外,用6个冬瓜品系作父本,6个南瓜品系作母本进行种内杂交。种内杂交组合比种间杂交组合具有更高的单果种子数。单果种子数以HMO2 × OMO2(359颗)和OMO5 × HMO8(314颗)组合最高。所有杂交瓜根组合的下胚轴长度为32.96 mm (RS17) ~ 73.35 mm (RS8),下胚轴厚度为2.71 mm (RS2) ~ 3.55 mm (RS8)。冬瓜和南瓜种内杂交嫁接成功率在96.6 ~ 100%之间,种间杂交嫁接成功率在87.1 ~ 100%之间。种内和种间杂交瓜系的根构型参数(根长、根体积、平均根径、根表面积和根干物质重)差异显著。研究结果表明,在砧木育种计划中培育的种内和种间的葫芦砧木候选品种有望作为嫁接甜瓜幼苗的商业砧木。今后将继续研究选育的种内和种间杂交瓜砧木候选品种对嫁接甜瓜的早熟性、果实品质和产量参数的影响。 关键词:大葫芦;Cucurbita moschata;根茎繁殖;根系结构;瓜# x0D;
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引用次数: 0
Effect of 1-methylcyclopropene on jackfruit with marketing simulation at 8°C 1-甲基环丙烯对8℃市场模拟菠萝蜜的影响
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-09 DOI: 10.9755/ejfa.2023.3150
David Antonio Morelos-Flores, Martina Alejandra Chacón-López, Efigenia Montalvo-González, María de Lourdes García-Magaña, Yolanda Nolasco-González, Luis Martín Hernández-Fuentes, Héctor González-Hernández
Jackfruit is a tropical fruit and competes with traditional crops such as mango, banana, and pineapple with regard to the quantity exported from the state of Nayarit, Mexico. Because jackfruit is a climacteric fruit with a high respiration rate and ethylene production, it has a limited market due to its short shelf life. The effect of the application of 1-methylcyclopropene (1-MCP) post-harvest to extend the shelf life of jackfruit was studied. Physiologically mature fruits were used and 1-MCP was applied at concentrations of 300, 600, and 1000 nL·L−1; control fruit without treatment was also evaluated. The fruits were stored for 5 days at 8°C and then were stored at 25°C to simulate the commercialization of the fruit. An absolute control group stored permanently at 25°C was used. The following analyses were carried out: physiologic and physicochemical factors, antioxidant capacity, vitamin C, total carotenoids, total soluble phenols, and sensory evaluation of the fruits when ripe. The climacteric peak for the absolute controls and fruit treated with 1-MCP (300, 600, and 1000 nL·L−1) occurred on days 3, 14, 17, and 17 respectively, prolonging the climacteric peak by an average of 13 days. The maximum rates of ethylene production were reported on days 3, 15, 17, and 17 in the same order, prolonging this by an average of 13 days. The treatment with 1-MCP (600 nL·L−1) extended the shelf life of jackfruit by 9 days compared with the absolute control, preserving the physicochemical, phytochemical, and sensory characteristics for up to 17 days of storage, confirming that the application of 1-MCP post-harvest is effective, providing the opportunity to increase the export destinations of Mexican jackfruit. Keywords: Jackfruit; 1-Methylcyclopropene; Marketing conditions; Physiologic and physicochemical parameters; Total soluble phenols; Antioxidant capacity
菠萝蜜是一种热带水果,在从墨西哥纳亚里特州出口的数量上与芒果、香蕉和菠萝等传统作物竞争。由于菠萝蜜是一种具有高呼吸速率和乙烯产量的更年期水果,由于其保质期短,市场有限。研究了菠萝蜜采后施用1-甲基环丙烯(1-MCP)对延长菠萝蜜保质期的影响。选用生理成熟的果实,分别施用300、600和1000 nL·L−1浓度的1- mcp;对未经处理的对照果实也进行了评价。将水果在8°C下保存5天,然后在25°C下保存,模拟水果的商业化。25℃永久保存的绝对对照组。对果实的生理、理化因素、抗氧化能力、维生素C、总类胡萝卜素、总可溶性酚以及果实成熟后的感官评价进行了分析。绝对对照和1- mcp(300、600和1000 nL·L−1)处理的果实分别在第3天、第14天、第17天和第17天达到更年期高峰,平均延长了13天。乙烯产率最高的时间依次为第3、15、17和17天,平均延长了13天。与绝对对照相比,1- mcp (600 nL·L−1)处理将菠萝蜜的保质期延长了9天,保留了菠萝蜜的物理化学、植物化学和感官特征长达17天的储存时间,证实了1- mcp在收获后的应用是有效的,为增加墨西哥菠萝蜜的出口目的地提供了机会。 关键词:菠萝蜜;1-Methylcyclopropene;营销环境;生理、理化参数;总可溶性酚;抗氧化能力
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引用次数: 0
Microwave-assisted puffing of Dendrobium officinale for higher extraction rate of polysaccharides 微波辅助膨化法提高铁皮石斛多糖提取率
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-05 DOI: 10.9755/ejfa.2023.3148
Yanhong Li, Zuoqiang Wang, Tingting Li, Xishan Bai, Kai Tian, Xiangzhong Huang
In order to increase the extraction rate of Dendrobium officinale polysaccharides, microwave puffing was used to process its fresh stems and the processing parameters were optimized. Response surface methodology was used to analyze the effects of sample length (1–5cm), moisture content (15%–25%), microwave power (550–790 W), and microwave processing time (20–40 s) on the expansion ratio. The results showed that the optimized expansion ratio of 371.7% was achieved at the following conditions, i.e., sample length of 3.5 cm, moisture content of 23%, microwave power of 706 W, and processing time of 35 s. It was found that polysaccharides were extracted more readily from puffed D. officinale than from non-puffed D. officinale by 41.8%. Moreover, the bioactivities of polysaccharides from puffing D. officinale and non-puffing D. officinale were evaluated and compared in lipid peroxidation inhibition and anti-hyperglycemic assays. Keywords: Dendrobium officinale; Microwave puffing; Polysaccharides; Lipid peroxidation; Anti-hyperglycemic activity
为了提高铁皮石斛多糖的提取率,采用微波膨化法对铁皮石斛鲜茎进行加工,并对工艺参数进行了优化。采用响应面法分析试样长度(1 ~ 5cm)、含水率(15% ~ 25%)、微波功率(550 ~ 790w)、微波处理时间(20 ~ 40s)对膨胀比的影响。结果表明,在样品长度为3.5 cm、含水率为23%、微波功率为706 W、处理时间为35 s的条件下,膨胀率为371.7%。结果表明,膨化铁皮石斛多糖提取率比未膨化铁皮石斛高41.8%。此外,我们还比较了膨化和非膨化铁皮水仙多糖在抑制脂质过氧化和抗高血糖方面的活性。 关键词:铁皮石斛;微波膨化;多糖;脂质过氧化作用;Anti-hyperglycemic活动
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引用次数: 0
Wheat plant response to zinc enrichment: results from a big plot assay 小麦植株对锌富集的反应:一个大型小区试验的结果
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-05 DOI: 10.9755/ejfa.2023.3149
Sara Rodrigo, Fernando J. Lidon, Fernando H. Reboredo, M. Manuela Silva, María M. Simões, A. Rita Costa
One third of the global population suffers Zn deficiency, which directly affects their health and the health-bill of all world countries. In this study, the application of Zn sulphate at the latest developing stages (anthesis and milk-dough stages) of bread wheat in Mediterranean conditions were tested for grain and leaves enrichment capacity and antioxidant activities prompting. Variety effects were found to be significant to success in enriching leaves and grains with Zn. While Almansor and Roxo varieties increased more than 50% and close to 40% respectively their amount in grain Zn, no significant differences were found for Paiva variety with or without Zn treatment. Regarding the leaves, Zn amount increases of 110, 230 and 300 ppm of Zn were figured out in Almansor, Roxo and Paiva varieties respectively. Antioxidant compounds in leaves showed to be higher when zinc treatment was applied in Almansor variety. Quality traits of the grain wheat were barely affected by the increase of the Zn amount; Roxo variety grains increased the protein and the dry gluten concentration, while Almansor grains were heavier and Almansor flour showed higher tenacity. It can be concluded that Zn enrichment in wheat plants caused variation in both grain and leaves mineral profile, and antioxidants in leaves. Keywords: Antioxidants, Rainfed conditions, RXF, Winter cereals, Zinc
全球三分之一的人口患有缺锌症,这直接影响到人们的健康和世界各国的卫生账单。研究了在地中海条件下面包小麦发育后期(开花期和制乳期)施用硫酸锌对籽粒和叶片的富集能力和抗氧化活性的促进作用。品种效应对叶片和籽粒锌的富集有显著影响。Almansor和Roxo的籽粒锌含量分别增加了50%以上和接近40%,而Paiva的籽粒锌含量在加锌和不加锌的情况下差异不显著。在叶片方面,Almansor、Roxo和Paiva分别增加了110、230和300 ppm的Zn。锌处理对苜蓿叶片中抗氧化物质含量的影响较大。锌用量的增加对籽粒小麦品质性状影响不大;Roxo籽粒提高了蛋白质含量和干面筋浓度,Almansor籽粒较重,Almansor面粉韧性较高。由此可见,锌的富集引起了小麦籽粒和叶片矿物组分的变化,以及叶片抗氧化剂的变化。关键词:抗氧化剂,旱作条件,RXF,冬季谷物,锌
{"title":"Wheat plant response to zinc enrichment: results from a big plot assay","authors":"Sara Rodrigo, Fernando J. Lidon, Fernando H. Reboredo, M. Manuela Silva, María M. Simões, A. Rita Costa","doi":"10.9755/ejfa.2023.3149","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3149","url":null,"abstract":"One third of the global population suffers Zn deficiency, which directly affects their health and the health-bill of all world countries. In this study, the application of Zn sulphate at the latest developing stages (anthesis and milk-dough stages) of bread wheat in Mediterranean conditions were tested for grain and leaves enrichment capacity and antioxidant activities prompting. Variety effects were found to be significant to success in enriching leaves and grains with Zn. While Almansor and Roxo varieties increased more than 50% and close to 40% respectively their amount in grain Zn, no significant differences were found for Paiva variety with or without Zn treatment. Regarding the leaves, Zn amount increases of 110, 230 and 300 ppm of Zn were figured out in Almansor, Roxo and Paiva varieties respectively. Antioxidant compounds in leaves showed to be higher when zinc treatment was applied in Almansor variety. Quality traits of the grain wheat were barely affected by the increase of the Zn amount; Roxo variety grains increased the protein and the dry gluten concentration, while Almansor grains were heavier and Almansor flour showed higher tenacity. It can be concluded that Zn enrichment in wheat plants caused variation in both grain and leaves mineral profile, and antioxidants in leaves.&#x0D; Keywords: Antioxidants, Rainfed conditions, RXF, Winter cereals, Zinc","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135368849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fungal inhibition by lactic acid bacteria (LAB) is modulated in vitro by cocoa fermentation-related conditions: towards a biocontrol of fungi in processing cocoa 乳酸菌(LAB)对真菌的抑制作用在体外由可可发酵相关条件调节:在可可加工过程中对真菌的生物防治
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-01 DOI: 10.9755/ejfa.2023.3146
Honoré Ouattara, Wilfried Yao, Hadja Ouattara, Karou Germain, Niamke Sebastien
Fungal growth impair strongly the quality of processed cocoa, leading to low crops value for farmers and manufacturers. The antifungal activity of lactic acid bacteria (LAB) from fermenting cocoa was analyzed and the conditions of fungal inhibition were evaluated. Fungi were isolated from stored fermented and dried cocoa beans on sabouraud plate medium. The results showed that, LAB strongly inhibit fungi isolated from fermented cocoa beans notably Mucors and Aspergilli, with Lactobacillus plantarum inducing the stronger inhibition whereas Leuconostoc mesenteroides produced a weaker inhibition. Acids production assayed by HPLC was found to be not related to antifungal activity, since LAB strains producing strong antifungal activity were not necessarly the best acid producers. Maximum fungal inhibition occured at 35 °C in Lactobacillus plantarum and Leuconostoc mesenteroides but also at 30 °C in Lactobacllus casei. Likewise, a narrow acidic pH range (3.0-4.0) allowed full expression of fungal inhibtion in LAB analyzed that decreased progressively toward pH 7.0 and failed at alkalin pH. However, sucrose even at high content (8%) was found to have no effect on antifungal activity of LAB, but its bioconversion product glucose and fructose decreased this activity when concentrations were set at 6 et 2 %, respectively. All together, the results suggest that, LAB may effectively exert fungal inhibition in a narrow timing of cocoa fermentation, with pulp contained sugars as metabolic regulators of this inhibition. These results may contribute to a better management of LAB as starter culture for an efficient inhibition of fungal growth and prevention of cocoa contamination from mycotoxins. Key Words : Antifungal activity ; lactic acid bacteria ; cocoa fermentation ; inhibition variation; pH and temperature ; sugars content.
真菌的生长严重损害了加工可可的质量,导致农民和制造商的作物价值降低。对可可发酵过程中乳酸菌的抑菌活性进行了分析,并对抑菌条件进行了评价。在sabouraud平板培养基上,从发酵和干燥的可可豆中分离真菌。结果表明,乳酸菌对发酵可可豆中分离的真菌有较强的抑制作用,其中对植物乳杆菌的抑制作用较强,对肠系膜Leuconostoc的抑制作用较弱。高效液相色谱法测定的产酸量与抗真菌活性无关,因为抗真菌活性强的乳酸菌不一定是最好的产酸菌。植物乳杆菌和肠系膜白色乳杆菌在35°C时抑菌效果最好,而干酪乳杆菌在30°C时抑菌效果最好。同样,在较窄的酸性pH范围内(3.0-4.0),真菌抑制作用可以在LAB中充分表达,当pH值接近7.0时,真菌抑制作用逐渐降低,而在碱性pH下则失效。然而,即使在高含量的蔗糖(8%)中,也发现蔗糖对LAB的抗真菌活性没有影响,但当其生物转化产物葡萄糖和果糖的浓度分别设定为6%和2%时,这种活性也会降低。综上所述,结果表明,乳酸菌可能在可可发酵的一个狭窄时间内有效地发挥真菌抑制作用,果肉中含有糖作为这种抑制的代谢调节剂。这些结果可能有助于更好地管理乳酸菌作为发酵剂,以有效抑制真菌生长和防止真菌毒素污染可可。 关键词:抗真菌活性;乳酸菌;可可发酵;抑制变异;pH和温度;糖含量。
{"title":"Fungal inhibition by lactic acid bacteria (LAB) is modulated in vitro by cocoa fermentation-related conditions: towards a biocontrol of fungi in processing cocoa","authors":"Honoré Ouattara, Wilfried Yao, Hadja Ouattara, Karou Germain, Niamke Sebastien","doi":"10.9755/ejfa.2023.3146","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3146","url":null,"abstract":"Fungal growth impair strongly the quality of processed cocoa, leading to low crops value for farmers and manufacturers. The antifungal activity of lactic acid bacteria (LAB) from fermenting cocoa was analyzed and the conditions of fungal inhibition were evaluated. Fungi were isolated from stored fermented and dried cocoa beans on sabouraud plate medium. The results showed that, LAB strongly inhibit fungi isolated from fermented cocoa beans notably Mucors and Aspergilli, with Lactobacillus plantarum inducing the stronger inhibition whereas Leuconostoc mesenteroides produced a weaker inhibition. Acids production assayed by HPLC was found to be not related to antifungal activity, since LAB strains producing strong antifungal activity were not necessarly the best acid producers. Maximum fungal inhibition occured at 35 °C in Lactobacillus plantarum and Leuconostoc mesenteroides but also at 30 °C in Lactobacllus casei. Likewise, a narrow acidic pH range (3.0-4.0) allowed full expression of fungal inhibtion in LAB analyzed that decreased progressively toward pH 7.0 and failed at alkalin pH. However, sucrose even at high content (8%) was found to have no effect on antifungal activity of LAB, but its bioconversion product glucose and fructose decreased this activity when concentrations were set at 6 et 2 %, respectively. All together, the results suggest that, LAB may effectively exert fungal inhibition in a narrow timing of cocoa fermentation, with pulp contained sugars as metabolic regulators of this inhibition. These results may contribute to a better management of LAB as starter culture for an efficient inhibition of fungal growth and prevention of cocoa contamination from mycotoxins.&#x0D; Key Words : Antifungal activity ; lactic acid bacteria ; cocoa fermentation ; inhibition variation; pH and temperature ; sugars content.","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Emirates Journal of Food and Agriculture
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