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Macroscopic study of the collection of human fetuses from Granada University 从格拉纳达大学收集的人类胎儿的宏观研究
Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-11-01 DOI: 10.52083/nfxy2293
Kevin Doello, Cristina Mesas, Francisco Quiñonero, Gloria Perazzoli, Consolación Melguizo, Miguel Guirao, Jose Prados
The anatomical collections of fetuses are a valuable element of study and research in universities. At the University of Granada, the Department of Human Anatomy and Embryology has a collection of 283 fetuses. The purpose of this article has been to carry out an exhaustive study on these fetuses to present data related to the malformations they presented. Regarding the results, it is worth noting the presence of a higher frequency of aborted fetuses between 4.5 and 6 months of development and the presence of macroscopically evident malformations in 56% of them. In conclusion, this study assesses the usefulness for teaching and research of this anatomical material and the results provide data of interest on fetal development and the etiology of spontaneous abortions.
胎儿的解剖收藏是大学学习和研究的重要组成部分。在格拉纳达大学,人体解剖与胚胎学系收集了283个胎儿。本文的目的是对这些胎儿进行详尽的研究,以提供与他们所呈现的畸形有关的数据。关于结果,值得注意的是,在发育4.5至6个月的胎儿中存在较高的流产频率,其中56%存在明显的宏观畸形。总之,本研究评估了该解剖材料对教学和研究的有用性,并为胎儿发育和自然流产的病因学提供了有趣的数据。
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引用次数: 0
Is there a change in ischiofemoral space in lateral position in comparison to supine 与仰卧位相比,侧位坐骨股骨间隙是否有变化
Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-11-01 DOI: 10.52083/oaqd8529
H. Abdolmohammadpour Bonab, D. Treytak, C. Azzopardi, Karthikeyan. P. Iyengar, Rajesh Botchu
Ischiofemoral impingement (IFI) is caused due to impingement of soft tissues between the ischium and the lesser trochanter of the femur, resulting in pain and discomfort. IFI can be caused due to anatomical abnormality, previous trauma and surgery. The quadratus femoris muscle is usually affected in these cases, which can become oedematous and show fatty atrophy. Management of IFI include analgesics, physiotherapy, image-guided intervention and surgery. This study aims to provide an overview of IFI, evaluate the ischiofemoral space in a cohort of ten patients, and suggest the safest position for image-guided interventions in patients with IFI. The ischiofemoral space and distance between the sciatic nerve and the medial part of the femoral neck were measured in supine and lateral positions on 10 consecutive patients by two fellowship-trained musculoskeletal radiologists on Computed Tomography (CT) axial sequence. There were 10 patients in the study (3 female and 7 males), with an average age of 29 years (11-70 years). A statistically significant increase in the ischiofemoral space and the distance between the sciatic nerve and the medial part of the femoral neck was found in the lateral position compared to the supine position. There was good intra- and inter-observer reliability, with a kappa value of 0.8. The increase in the ischiofemoral interval in the lateral position suggests that it is relatively safer to perform image-guided interventions and injections in the ischiofemoral interval in the lateral position with a potential reduction in the risk of incidental sciatic nerve injury.
坐骨股骨撞击(ischiofemounal impingement, IFI)是由于坐骨与股骨小粗隆之间的软组织受到撞击而引起的,导致疼痛和不适。IFI可由解剖异常、既往创伤及手术所致。在这些病例中,股方肌通常受到影响,可能会水肿并表现为脂肪萎缩。IFI的治疗包括止痛、物理治疗、图像引导干预和手术。本研究旨在概述IFI,评估10例患者的坐骨股骨空间,并建议IFI患者进行图像引导干预的最安全位置。连续10例患者采用仰卧位和侧卧位测量坐骨股骨间隙和坐骨神经与股骨颈内侧之间的距离,由两名训练有素的肌肉骨骼放射科医生使用计算机断层扫描(CT)轴向序列测量。本研究共纳入10例患者,其中女性3例,男性7例,平均年龄29岁(11-70岁)。与仰卧位相比,侧卧位坐骨股骨间隙和坐骨神经与股骨颈内侧之间的距离有统计学意义的增加。有良好的观察者内部和观察者之间的信度,kappa值为0.8。侧位坐骨股间段的增加表明,在侧位坐骨股间段进行图像引导干预和注射相对更安全,并有可能降低坐骨神经意外损伤的风险。
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引用次数: 0
Comparative assessment of the morphology and antigenicity of human osteochondral units using formalin and coagulant fixatives 使用福尔马林和凝固固定剂对人骨软骨单位形态和抗原性的比较评估
Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-11-01 DOI: 10.52083/cvzf3774
Ganesh Parasuraman, Elizabeth Vinod, Abel Livingston, Solomon Sathishkumar, Deepak Vinod Francis
Fixation is crucial for preserving tissue integrity during processing, and the most commonly used cross-linking fixative in immunohistochemistry is neutral buffered formalin, which requires antigen retrieval as a crucial step. The successful use of newer coagulant fixatives like methacarn and EMA to preserve isotopes and eliminate the need for antigen retrieval has been reported recently, but their role in decalcified osteochondral unit samples remains unknown. Limited information on the use of coagulant fixatives as formalin substitutes makes it important to comparatively evaluate their effects on osteochondral samples and the impact of antigen retrieval on different days. Osteochondral units from a patient with osteoarthritis who underwent total knee-replacement surgery were fixed with three studied fixatives (Formalin, Methacarn, EMA) and divided into four portions, for different time periods (Day 1, 3, 7, 10). Sections were decalcified, stained with Safranin O, Alcian Blue, Toluidine Blue, PicroSirius Red, Hematoxylin and Eosin, and immunohistochemical analysis of Collagen type II and type X with and without antigen retrieval was conducted. Formalin showed better hematoxylin uptake than coagulant-based fixatives, while all fixatives preserved tissue morphology without necrosis or cellular degeneration with comparable staining quality. Methacarn and EMA-fixed tissues showed higher uptake of collagen type II compared to formalin-fixed tissues, with collagen type II uptake occurring only in the cartilage region and collagen type X uptake occurring only in the bone region. The study highlights the effectiveness of methacarn and EMA as efficient alternatives to formalin, preserving tissue morphology and antigen specificity.
固定对于在加工过程中保持组织完整性至关重要,免疫组织化学中最常用的交联固定剂是中性缓冲福尔马林,这需要抗原回收作为关键步骤。最近有报道成功地使用了新型凝血固定剂,如methacarn和EMA来保存同位素并消除抗原提取的需要,但它们在脱钙骨软骨单位样本中的作用尚不清楚。关于使用凝固固定剂作为福尔马林替代品的信息有限,因此比较评估其对骨软骨样品的影响以及不同日期抗原检索的影响非常重要。对接受全膝关节置换术的骨关节炎患者的骨软骨单位使用三种所研究的固定剂(福尔马林、美沙卡恩、EMA)进行固定,并在不同时间段(第1、3、7、10天)分为四部分。切片脱钙,用红花红O、阿利新蓝、甲苯胺蓝、小天狼星红、苏木精和伊红染色,对II型和X型胶原进行免疫组化分析,并进行抗原回收和不进行抗原回收。福尔马林比基于凝固剂的固定液表现出更好的苏木精吸收,而所有固定液都保留了组织形态,没有坏死或细胞变性,染色质量相当。与福尔马林固定组织相比,Methacarn和ema固定组织显示出更高的II型胶原摄取,II型胶原摄取仅发生在软骨区域,X型胶原摄取仅发生在骨区域。该研究强调了甲沙康和EMA作为福尔马林的有效替代品的有效性,保留了组织形态和抗原特异性。
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引用次数: 0
Parahiatal hernia: A rare type of hernia and the answer to an anatomical challenge 裂孔旁疝:一种罕见类型的疝和答案解剖的挑战
Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-11-01 DOI: 10.52083/yjge5299
Fátima Senra Lorenzana, Asunción Acosta Mérida, Noman Zafar, Alberto Martínez Isla
Parahiatal hernias are rare. They are difficult to diagnose preoperatively, as the clinical symptoms may be similar to hiatal and paraoesophageal hernias. Here, we report two cases of parahiatal hernia that were preoperatively diagnosed and successfully repaired laparoscopically; using the particular anatomic characteristics of this hernia, we also review the controversial oesophageal hiatal anatomy, as the surgical community often refers to the left bundle of the right crus as the left crus. There is no consensus on the indication or surgical technique to repair them. The first case is a 59-year-old woman with non-specific abdominal symptoms, in whom the preoperative gastroscopy and computed tomography (CT) raised the suspicion for parahiatal hernia. The second case is a 68-year-old woman who presented to the emergency department with abdominal distention and nausea, but no vomiting. Preoperative CT raised the suspicion of an incarcerated parahiatal hernia. Both patients underwent laparoscopic repair of the parahiatal hernia and a Toupet fundoplication. They had an uneventful postoperative course. After more than 4 years of follow-up, they are both asymptomatic. Parahiatal hernias are a rare form of diaphragmatic hernia that occur through a diaphragmatic defect lateral to an anatomically normal oesophageal hiatus, with herniation of contents between the left portion of the right crus and the left crus. Up to five different anatomical variations have been described. The knowledge of these anatomical variations has an impact on the type of surgical repair that will need to be performed if a parahiatal hernia is found.
腹旁疝是罕见的。由于临床症状可能与食管裂孔疝和食管旁疝相似,故术前诊断困难。在此,我们报告两例经术前诊断并经腹腔镜成功修复的食管旁疝;利用这种疝气的特殊解剖特征,我们也回顾了有争议的食管裂孔解剖,因为外科界经常将右小腿的左束称为左小腿。对于修复它们的适应证或手术技术尚无共识。第一例患者为59岁女性,腹部无特异性症状,术前胃镜检查和CT检查怀疑为裂孔旁疝。第二个病例是一名68岁的妇女,她以腹胀和恶心就诊于急诊科,但没有呕吐。术前CT提示怀疑嵌顿性腹旁疝。两例患者均行腹腔镜下食管旁疝修补术和Toupet手术。他们的术后过程平安无事。经过4年多的随访,两人均无症状。裂孔旁疝是一种罕见的膈疝,发生在解剖上正常的食管裂孔外侧的膈缺损,右小腿和左小腿之间的内容物突出。多达五种不同的解剖变异已被描述。如果发现腹旁疝,对这些解剖变异的了解会影响手术修复的类型。
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引用次数: 0
The behavior of adrenal progenitor stem cells in response to chronic stress and recovery 肾上腺祖干细胞对慢性应激和恢复的反应
Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-11-01 DOI: 10.52083/imov9344
Nermeen H.A. Lashine, Amany Shams, Shaima M. Almasry, Huda El-Tahry, Marwa Abd El-kader
Stressors affect the differentiation of stem cells in the hypothalamic-pituitary-adrenal axis (HPA). This study was designed to ensure the presence of progenitor cells in the adult adrenal gland and to evaluate their behavior under chronic stress and after recovery. Also, to assess their ability to recruit new glial and chromaffin cells. Three groups of adult male rats (6 rats each): control, chronic-stress (rats were placed individually in stainless restrainers 2h/day for six days), recovered (rats were housed in an enriched circumference for seven days after the same stress modality). Both chronic-stress and recovered groups showed increased adrenal glands weight and cortisol levels with vacuolation, hemorrhage, and edema in the cortex and medulla. The chronic-stress group illustrated a significant increase in the chromaffin reaction, which was reduced in the recovered group. Evaluation of the immunohistochemical results revealed a significant decrease in the Nestin and GFAP (glial fibrillary acidic protein) reactions, but an increase in the chromogranin-A reaction in the chronic-stress group. The recovered group demonstrated a significant increase in the Nestin and GFAP and a reduction in the chromogranin-A immunohistochemical reactions. These results indicate the differentiation of the progenitor (Nestin expressing) stem cells into chromaffin (chromogranin-A expressing) cells under stress conditions for stress adaptation. Conversely, under normal conditions, the differentiation moved toward the glial cells.
应激源影响下丘脑-垂体-肾上腺轴(HPA)干细胞的分化。本研究旨在确保成人肾上腺中祖细胞的存在,并评估它们在慢性应激和恢复后的行为。同时,评估它们招募新神经胶质细胞和染色质细胞的能力。三组成年雄性大鼠(每组6只):对照组、慢性应激组(将大鼠单独置于不锈钢约束器中,每天2小时,连续6天)、康复组(在相同的应激模式下,将大鼠置于富集周长7天)。慢性应激组和康复组均显示肾上腺重量和皮质醇水平增加,皮质和髓质出现空泡、出血和水肿。慢性应激组的染色质反应明显增加,而恢复组的染色质反应减少。免疫组化评价结果显示,慢性应激组Nestin和GFAP(胶质纤维酸性蛋白)反应显著降低,但嗜铬粒蛋白a反应增加。恢复组Nestin和GFAP显著升高,嗜铬粒蛋白a免疫组化反应明显降低。这些结果表明,在应激条件下,祖细胞(表达巢蛋白)干细胞分化为染色质(表达嗜铬粒蛋白- a)细胞以适应应激。相反,在正常情况下,分化向胶质细胞转移。
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引用次数: 0
Morphometric analysis of cavitas glenoidalis with multidedector CT 关节腔多探头CT形态计量学分析
Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-11-01 DOI: 10.52083/mrfp5453
Ali Keleş, Mehmet T. Yilmaz, Orhan Özbek, Duygu A. Saygin, Muzaffer Şeker
The glenoid cavity is a sliced egg-shaped joint surface located on the lateral margin of the scapula to form the shoulder joint. Recognition of variations in shape and dimensions of the glenoid cavity is important for a better comprehension of joint-associated diseases, especially in total shoulder arthroplasty procedures. The aim of this study was to perform morphometric measurements on the glenoid cavity. Glenoid cavities of 391 individuals (197 males [50.4%], 194 females [49.6%]) were reviewed by using Multi-detector Computed Tomography. The maximum length and maximum width of glenoid cavities, as well as the width, depth and circumference at the notch level were measured, and the index value was calculated. The glenoid cavity shapes were typed as pear, inverted comma and oval type. Furthermore, the metric values that provide the best differentiation between genders were identified through ROC analysis. The pear glenoid cavity type was detected in 53.2%, inverted comma type was detected in 28.4%, and oval type was detected in 18.4% of cases. In all of our morphometric measurements, male values were higher than female values, and there was significant difference between them. Results of ROC analysis revealed significant measurements for the maximum length and maximum width measurements of the glenoid for gender determination. Morphometric information of the glenoid cavity can be useful in order to increase clinical success in case of Bankart lesion, rotator cuff disease, and osteochondral defect. Recognition of different shapes and dimensions of the glenoid cavity is essential for the design of the glenoid component, especially for total shoulder arthroplasty procedure. We believe that the data obtained in our study would be useful for prosthesis designers and orthopaedic surgeons.
肩胛盂是位于肩胛骨外侧缘形成肩关节的一个切开的蛋形关节面。识别关节盂形状和尺寸的变化对于更好地理解关节相关疾病非常重要,特别是在全肩关节置换术中。本研究的目的是对关节盂进行形态测量。本文对391例患者(男性197例[50.4%],女性194例[49.6%])进行了多探测器计算机断层扫描。测量关节盂的最大长度和最大宽度,以及关节盂缺口处的宽度、深度和周长,并计算指标值。关节盂形状分为梨型、倒逗号型和椭圆形。此外,通过ROC分析确定了提供最佳性别区分的度量值。梨盂型占53.2%,逗号型占28.4%,椭圆形型占18.4%。在我们所有的形态计量测量中,男性值高于女性值,两者之间存在显著差异。ROC分析结果显示,肩关节的最大长度和最大宽度测量对于性别的确定具有重要意义。在Bankart病变、肩袖疾病和骨软骨缺损的病例中,肩胛盂的形态测量信息对于提高临床成功率是有用的。识别不同形状和尺寸的盂内腔是必不可少的盂内假体的设计,特别是在全肩关节置换术。我们相信在我们的研究中获得的数据将是有用的假体设计者和矫形外科医生。
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引用次数: 0
Prevalence of Morton’s toe and assessment of the associated risk factors: a cross-sectional study 莫顿趾的患病率和相关危险因素的评估:一项横断面研究
Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-11-01 DOI: 10.52083/zojg4094
Bhagath K. Potu, Bader M.M. Saleem, Amer Almarabheh
Morton’s toe results in an uneven distribution of pressure across the feet. Awareness about Morton’s toe and its associated risk factors is clinically important. Although studies have reported the prevalence of Morton’s toe, not many studies appear in literature discussing its impact on the associated risk factors such as hammertoe, bunion deformity, overpronation and plantar fasciitis, respectively. Hence, we have undertaken this study to analyze the prevalence of Morton’s toe in the Gulf region to assess its impact on the foot. A cross-sectional descriptive epidemiological study was carried out between November 2021 and December 2022 on the feet of 100 individuals (male=50; female=50) with ages ranging from 17 to 75 years. The study included the Bahraini & non-Bahraini residents. Individuals with severe foot injuries and deformities resulting from surgery were excluded. A total of 56 Morton’s toe cases (male=18; female=38) were noted in the study sample. The overall prevalence of Morton’s toe was 28% and majority of the participants with Morton’s toe were females (67.9%). Of 56 Morton’s toe cases, the majority were seen in the age group from 30 to 40 years, particularly in females. Our findings clearly showed a significant association between Morton’s toe and hammertoe (p=0.044), bunion and usage of high heels (p<0.001), respectively. However, the association between Morton’s toe and other risk factors such as plantar fasciitis and overpronation of the foot were found to be statistically nonsignificant (p>0.05). Our study provides baseline data for the first time on the prevalence of Morton’s toe and associated risk factors.
莫顿的脚趾导致压力在脚上的不均匀分布。对莫顿趾及其相关危险因素的认识在临床上具有重要意义。尽管有研究报道了莫顿趾的患病率,但文献中讨论其对槌状趾、拇外翻畸形、过旋和足底筋膜炎等相关危险因素影响的研究并不多。因此,我们进行了这项研究来分析莫顿趾在海湾地区的患病率,以评估其对足部的影响。在2021年11月至2022年12月期间,对100人的脚进行了横断面描述性流行病学研究(男性=50;女性=50),年龄从17岁到75岁不等。这项研究包括巴林和;non-Bahraini居民。有严重足部损伤和手术导致的畸形的个体被排除在外。莫顿氏趾56例(男18例;女性=38)被记录在研究样本中。莫顿趾的总体患病率为28%,其中女性占67.9%。在56例莫顿趾病例中,大多数发生在30至40岁年龄组,尤其是女性。我们的研究结果清楚地表明,莫顿趾和锤状趾(p=0.044)、拇外翻和使用高跟鞋(p= 0.001)之间分别存在显著的关联。然而,莫顿趾与足底筋膜炎、足过内旋等其他危险因素的相关性无统计学意义(p>0.05)。我们的研究首次提供了关于莫顿趾患病率和相关危险因素的基线数据。
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引用次数: 0
A cluster of dysmorphologies in a male human body: The value of anatomical variants in health sciences student training 男性人体畸形群:解剖学变异在健康科学学生训练中的价值
IF 0.3 Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-09-01 DOI: 10.52083/czaa2387
Pieter-Jan Bosman, Surasha M. Surandernath, Baron Quinton, Daniel Ziqubu, Muhammad Khan, Faatimah Asmal, B. Kramer
The human body is known to contain many variations in its normal structure which, while of interest to teachers of anatomy, may be vexing to health sciences students when compared to the description of “normal” anatomy in their textbooks. However, these variations, and even dysmorphologies, pose interesting and sometimes challenging learning experiences to students during dissection of the body. Such an instance occurred for undergraduate medical students in the School of Anatomical Sciences, University of the Witwatersrand, South Africa, when three unrelated dysmorphologies were discovered while undertaking a full dissection of a donor’s body. An aberrant right subclavian artery was found in the thoracic cavity and two further dysmorphologies, a supernumerary kidney and accessory indentations on the diaphragmatic surface of the liver presented on dissection of the abdominal cavity. The aberrant right subclavian conformed with previous descriptions of the anomaly. However, the supernumerary kidney lacked a ureter, was lobulated and contained large blood-filled spaces, with histological evidence of urinary tubules in the intervening connective tissue. The accessory hepatic indentations varied in depth, with the deeper one forming a fissure and the less deep indentation, a sulcus. While the described dysmorphologies vary in their incidence, the occurrence of a cluster of three within one body provided a significant opportunity for the students to review the normal anatomy, and especially the complex development of the structures, as well as the clinical significance of each.
众所周知,人体在其正常结构中包含许多变化,尽管解剖学教师对此感兴趣,但与教科书中对“正常”解剖学的描述相比,健康科学专业的学生可能会感到烦恼。然而,这些变异,甚至畸形,在解剖身体的过程中,给学生带来了有趣的,有时是具有挑战性的学习经历。这样的例子发生在南非威特沃特斯兰德大学解剖科学学院的医科本科生身上,当时在对捐赠者的身体进行全面解剖时发现了三个不相关的畸形。在胸腔中发现了一条异常的右锁骨下动脉,在腹腔解剖中发现了两个进一步的畸形,一个多余的肾脏和肝脏横膈膜表面的附属凹陷。右锁骨下异常符合先前对异常的描述。然而,多余肾脏缺少输尿管,呈分叶状,含有大的充满血液的间隙,组织学证据表明在中间结缔组织中有尿小管。副肝凹陷深度不同,深的形成裂隙,浅的形成沟。虽然所描述的畸形的发生率各不相同,但在一个身体中出现三种畸形的集群为学生提供了一个重要的机会来回顾正常解剖,特别是结构的复杂发展,以及每种畸形的临床意义。
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引用次数: 0
Lycopene attenuates oxidative stress, apoptosis, and biochemical fluctuations induced by bisphenol A in the kidney of rats 番茄红素可减轻双酚A引起的大鼠肾脏氧化应激、细胞凋亡和生化波动
IF 0.3 Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-09-01 DOI: 10.52083/fgzo9858
Mohammed Alorini
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引用次数: 0
Role of mesenchymal stem cells and taurine in chronic pancreatitis in adult albino rats 间充质干细胞和牛磺酸在成年白化大鼠慢性胰腺炎中的作用
IF 0.3 Q4 ANATOMY & MORPHOLOGY Pub Date : 2023-09-01 DOI: 10.52083/jklg9096
Nahla S. Saad, Ghada S. El-dien Abdelkader, Noha A.H. Salem, M. H. Mohammed Ali, M. El-Fark
Chronic pancreatitis (CP) is an inflammatory disease of the pancreas that leads to pancreatic fibrosis. The current treatment of the disease is not efficient or adequate. Therefore, more efficient interventions are required to diminish the substantial burden of the disease. The present study aimed to assess the potential therapeutic value of bone marrow-derived mesenchymal stem cells (BMSCs) and/or taurine supplementation in CP-induced, using intraperitoneal injection of L-arginine. Forty-five rats were randomly divided into five groups (9 rats each): 1) control group, 2) CP group, 3) CP+BMSCs, 4) CP+Taurine, and 5) CP+BMSCs+Taurine. At the end of the experimental period, the pancreatic tissues were collected, weighed, and prepared for light, electron, and immunohistochemical (α-SMA) microscopic examination. The CP group showed destruction of the pancreatic tissues including fatty degeneration, minimal zymogen granules, and focal degranulation of the rER. Some of the islets degenerated with intense immunoreactivity of α-SMA in the stroma. The groups treated with BMSCs or taurine alone showed improvement of the pancreatic architecture with the presence of some cytoplasmic vacuolation, fewer zymogen granules than the control group, and minimal inflammatory cell infiltrate. The CP+BMSCs+Taurine group showed apparently normal architecture. The combined therapy of both BMSCs and taurine could ameliorate CP progression by suppressing inflammation and fibrosis.
慢性胰腺炎(CP)是胰腺的一种炎症性疾病,可导致胰腺纤维化。目前对这种疾病的治疗既不有效也不充分。因此,需要采取更有效的干预措施,以减轻该病的沉重负担。本研究旨在通过腹腔注射l -精氨酸,评估骨髓间充质干细胞(BMSCs)和/或补充牛磺酸对cp诱导的潜在治疗价值。将45只大鼠随机分为5组(每组9只):1)对照组,2)CP组,3)CP+BMSCs, 4) CP+牛磺酸,5)CP+BMSCs+牛磺酸。实验结束时,收集胰腺组织,称重,进行光镜、电镜和免疫组化(α-SMA)显微镜检查。CP组胰腺组织破坏,包括脂肪变性,酶原颗粒极少,内质网局灶性脱粒。部分胰岛变性,间质α-SMA免疫反应性强。单独用骨髓间充质干细胞或牛磺酸处理的组胰腺结构得到改善,出现了一些细胞质空泡化,酶原颗粒比对照组少,炎症细胞浸润最小。CP+BMSCs+牛磺酸组结构明显正常。BMSCs和牛磺酸联合治疗可以通过抑制炎症和纤维化来改善CP的进展。
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引用次数: 0
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European journal of anatomy
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