European Journal of Oral Sciences最新文献

In long bones, glucocorticoids are known to cause a decrease in bone formation and an increase in bone resorption, leading to a condition of osteoporosis also during growth. However, there are few studies on the effect of glucocorticoids on alveolar bone. The aim of the present study was to evaluate the impact of chronic administration of glucocorticoids on osteocytes and the lacuno-canalicular system of maxillary and mandibular alveolar bone in growing rats. Male Wistar rats were administered intramuscular dexamethasone (7 mg/kg b.w.) or saline solution once a week for 5 weeks. After euthanasia, mandibles and upper maxillae were resected and processed for micro-computed tomography, histological, and immunohistochemical evaluation. Glucocorticoids induced morphological changes in the lacuno-canalicular system and apoptosis of alveolar bone osteocytes and had no effect on SOST/sclerostin osteocyte expression. Our findings suggest that the deleterious effect of glucocorticoids on alveolar bone is associated with osteocyte apoptosis and lacuno-canalicular system changes, whereas Wnt/β catenin pathway regulation in osteocytes appears to play no significant role in the pathogenesis of glucocorticoid-induced bone loss.

This systematic review evaluated the effects of coenzyme Q10 (CoQ10) in non-surgical periodontal treatment (PROSPERO: CRD42022311286). Five databases were screened (up to May/2024). It included only randomized controlled trials (RCTs) of subgingival scaling root planing (SRP) with or without adjunct use of the antioxidant CoQ10 in adults with periodontitis. The risk of bias and the certainty of the evidence were assessed. Meta-analyses were conducted to estimate mean differences for probing depth (PD) and clinical attachment level (CAL) between baseline and follow-up. Ten studies were included, of which four administered CoQ10 locally (topical [n = 1]; intra-pocket [n = 3] modalities), and six used oral supplementation. There was no significant effect of local use of CoQ10 on reduction of PD and gain in CAL. Daily oral supplementation (120 mg/day) with CoQ10 resulted in a greater mean reduction of PD by 0.41 mm (95% CI: 0.02–0.80) and a greater mean CAL gain by 0.52 mm (95% CI: 0.26–0.78) than seen in controls. Based on very low certainty of evidence, there was no significant effect of locally delivered Q10 gel on PD/CAL, but daily oral supplementation with CoQ10 resulted in better periodontal health after 12 weeks.

The periodontal ligament (PDL) is a connective tissue, and PDL cells have a potential to differentiate into cementoblasts, osteoblasts, and gingival fibroblasts. This study investigated whether transcription factor c-Myb could induce differentiation of PDL cells for periodontal regeneration. PDL cells were isolated from extracted teeth and cultured. c-Myb was transfected to PDL cells using replication-deficient adenoviral vector. Differentiation of the PDL cells was analyzed by immunoblot, alkaline phosphatase activity, Alizarin red stain, and immunofluorescence analysis. Cell viability on titanium surfaces was analyzed by crystal violet stain and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. PDL cells cultured in osteogenic medium showed increased production of osteogenic and cementogenic molecules. Moreover, c-Myb-transfected cells showed increased production of dentinogenic molecules, in addition to the osteogenic and cementogenic molecules, even in normal culture condition. c-Myb-transfected cells also exhibited increased autophagy and type I collagen production under nutrient deprivation. When grown on a titanium surface, c-Myb-transfected cells showed increased production of osteogenesis-, dentinogenesis-, and cementogenesis-related molecules and cell viability. Thus, these results suggest that c-Myb might play an essential role during periodontal regeneration by improving the differentiation of PDL cells, and c-Myb can be utilized for enhancing the attachment of PDL cells to dental implant surfaces.

The aims of this study were twofold: first, to investigate the surface roughness of different abutment materials prepared using various manufacturing methods; and, second, to evaluate colonization by Streptococcus mutans and Candida albicans according to abutment material and manufacturing method. Six material/manufacturing method combinations were investigated in this study, namely chromium–cobalt (Cr–Co) (prepared using casting, milling, and laser sintering) and titanium, zirconia, and anodized titanium (all prepared using milling); titanium (stock) abutments were used as the control group. Surface roughness of seven specimens from each group was evaluated using atomic force microscopy and scanning electron microscopy. Laser-sintered Cr–Co had the lowest values of R_a (mean ± SD = 4.8 ± 0.8  nm), R_q (mean ± SD = 7.0 ± 1.2 nm), and R_max (mean ± SD = 133.5 ± 31.7 nm), whereas milled zirconia had the highest values of R_a (mean ± SD = 112.9 ± 44.2 nm), R_q (mean ± SD = 142.8 ± 54.0 nm), and R_max (mean ± SD = 1,035.7 ± 350.4 nm). Three specimens from each group were infected with S. mutans and three with C. albicans, and microbial counts were evaluated after culture. Colonization of Streptococcus mutans was highest on milled zirconia (mean log₁₀ count ± SD = 5.87 ± 0.08) and lowest on milled Cr–Co (mean log₁₀ count ± SD = 4.04 ± 0.11). For C. albicans, colonization was highest on milled titanium stock (mean log₁₀ count ± SD = 6.62 ± 0.03) and lowest on milled anodized titanium (mean log₁₀ count ± SD = 6.13 ± 0.03). Differences in surface roughness and microbial colonization among groups can aid clinicians in selecting materials based on clinical relevance, considering their potential impact on outcomes.

There are no interventional studies on the oral health of immigrant children in Norway. This study aimed to assess the effectiveness of a culturally adapted oral health intervention for parental knowledge, attitude to oral hygiene, attitude to diet, and indulgence (permissiveness toward oral hygiene and sugar intake) concerning their children's oral health. A cluster randomized control trial, including immigrant parents of newborns, was conducted at seven health centers in Bergen (Norway) involving a 2-session one-to-one motivational interviews, toothbrushing demonstrations and pamphlets in the participants’ native languages at baseline and after 6 months. Mean differences in outcome scores were assessed and compared between intervention and control groups using generalized linear mixed model. In an intention-to-treat (ITT) analysis (n = 345), the estimated mean difference in parental attitude to diet and indulgence between the groups indicated more improvement in the control than in the intervention group in the adjusted models. For all outcomes within both groups, a slight positive change was observed in marginal mean scores from baseline to follow-up. The two culturally adapted intervention sessions had no impact on oral health-related knowledge, attitudes, and indulgence of immigrant parents. These findings suggest that more comprehensive interventions may be needed to improve parental knowledge and attitudes toward children's oral health.

Trial Registration number: ClinicalTrials.gov Identifier: NCT05758454

The main challenges to the widespread clinical application of three-dimensional (3D)-printed customized trays include cost and time limitations. This study examined how changing the internal structure of 3D-printed materials used for customized trays affects flexural strength (FS), flexural modulus (FM), manufacturing time, and material weight. Specimens (64 × 10 × 3.3 mm) were printed using a light-sensitive liquid resin. The internal structures of control specimens were completely filled, whereas the internal structures of test groups comprised vertical bars spaced 1 mm (Test 1) or 2 mm (Test 2) apart. Specimens were weighed and then subjected to a three-point bending test to evaluate their FS and FM. Data were analyzed using one-way ANOVA and Tukey's test, with Weibull analysis applied to FS values. Control specimens had the highest FS (106 ± 4 MPa), while Test 2 specimens demonstrated the highest FM (6101 ± 1407 MPa). No significant differences were found between Test 1 and Test 2 specimens in FS or FM. Test 2 specimens had the lowest mean weight (1440 ± 42 mg). Manufacturing times were 80 min for control and Test 1 specimens and 60 min for Test 2 specimens. Including spaces in the internal structure of 3D-printed custom tray material saves material and manufacturing time while maintaining mechanical properties.

Despite treatment advances, head and neck squamous cell carcinoma (HNSCC) still poses a significant global health challenge. Combination therapies have emerged as more effective strategies than traditional chemotherapy in clinical practice by improving tumor response rates and patient survival while minimizing treatment-related toxicity. This study investigates the anticancer effects of metformin and verteporfin (Yes-associated protein 1 [YAP1] inhibitor) alone or in combination in HNSCC using vitro and in vivo approaches. Quantitative RT-PCR analysis of HNSCC cell lines reveals upregulation of YAP1 and related genes in the Hippo signaling pathway. Cell viability assays demonstrate a beneficial synergistic effect between metformin and verteporfin in inhibiting HNSCC tumor growth. In male BALB/cAJcl-nu/nu mice harboring HNSCC tumor xenografts, intraperitoneal administration of metformin and verteporfin enhances the inhibitory effect on tumor growth and suppresses YAP1 nuclear translocation when compared to vehicle or monotherapies. Furthermore, combination of these drugs reduces tumor cell proliferation (marked by Ki-67) and inhibits phosphoS6 ribosomal protein, as observed through immunofluorescent and immunohistochemical (IHC) analyses. The in vivo study underscores the therapeutic potential of the dual targeting approach with metformin and verteporfin in treating HNSCC, with minimal toxicity.