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Analysis of Tissue Repair of a New Cement Based on Calcium and Strontium Aluminates: A Triple-Blinded, Randomized, Controlled Trial in an Animal Model. 基于钙和锶铝酸盐的新型水泥的组织修复分析:动物模型中的三盲随机对照试验。
Q1 Dentistry Pub Date : 2024-10-01 Epub Date: 2024-05-22 DOI: 10.1055/s-0044-1786875
Elizandra Silva da Penha, Nonato Amorim de Farias Filho, Luanna Abílio Diniz Melquíades de Medeiros, Rosana Araújo Rosendo, Marco Antônio Dias da Silva, Willams Teles Barbosa, Raúl García-Carrodeguas, Miguel Angel Rodríguez, Eliseu Aldrighi Münchow, Rogério Lacerda-Santos, Marcus Vinícius Lia Fook

Objective:  The focus of this triple-blind randomized study was to evaluate the biocompatibility of a new root canal filling sealer (RCFS) based on tristrontium aluminate and dodecacalcium hepta-aluminate in living tissue.

Material and methods:  Forty-five Wistar rats (Rattus norvegicus) were divided into three groups: control (polyethylene), sealer (Bio-C Sealer, Londrina, PR, Brazil), and experimental (tristrontium aluminate and dodecacalcium hepta-aluminate). The tissues were analyzed under an optical microscope to assess different cellular events at different time intervals (7, 15, and 30 days).

Statistical analysis:  Data were analyzed using the Kruskal-Wallis and Dunn (p < 0.05) tests.

Results:  In the initial period, a moderate inflammatory infiltrate was observed, similar between the endodontic cements groups (p = 0.725). The intensity of the infiltrate decreased with time, with no significant difference among the groups (p > 0.05). The number of young fibroblasts was elevated in all groups evaluated at 7 days. The experimental group showed the highest number of cells at all time intervals, but the difference with the sealer group at 7 (p = 0.001) and 15 days (p = 0.002) and the control group at 30 days was not significant (p = 0.001). Regarding tissue repair events, the amount of collagen fibers increased over the experimental intervals, with no significant difference between the sealer and control groups (p > 0.05).

Conclusion:  The experimental RCFS based on calcium and strontium aluminates proved to be biocompatible for use in close contact with periapical tissue, inducing a low inflammatory reaction and favoring rapid tissue repair.

研究目的这项三盲随机研究的重点是评估一种基于铝酸三锶和十二钙七铝酸盐的新型根管充填封闭剂(RCFS)在活组织中的生物相容性:将 45 只 Wistar 大鼠(Rattus norvegicus)分为三组:对照组(聚乙烯)、封闭剂组(Bio-C Sealer,巴西隆德里纳)和实验组(铝酸三锶和十二钙七铝)。在光学显微镜下对组织进行分析,以评估不同时间间隔(7 天、15 天和 30 天)的不同细胞事件:数据分析采用 Kruskal-Wallis 和 Dunn(P 结果:在初期,观察到中度炎症浸润,牙髓水泥组之间相似(p = 0.725)。随着时间的推移,浸润的强度有所下降,但各组间无明显差异(p > 0.05)。在 7 天的评估中,所有组的幼成纤维细胞数量都有所增加。实验组在所有时间间隔内细胞数量最多,但与封闭剂组 7 天(p = 0.001)和 15 天(p = 0.002)以及对照组 30 天(p = 0.001)的差异不显著(p = 0.001)。在组织修复事件方面,胶原纤维的数量在实验期间有所增加,但封闭剂组和对照组之间没有显著差异(p > 0.05):实验证明,基于铝酸钙和铝酸锶的 RCFS 与根尖周组织密切接触时具有良好的生物相容性,诱发的炎症反应较低,有利于组织的快速修复。
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引用次数: 0
Interprofessional Education in Saudi Arabia: A Call to Action. 沙特阿拉伯的跨专业教育:行动呼吁书。
Q1 Dentistry Pub Date : 2024-10-01 Epub Date: 2024-07-16 DOI: 10.1055/s-0044-1788321
Ahmed Yaseen Alqutaibi, Rawda Awad, Mohamed M Rahhal, Muhammad S Zafar
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引用次数: 0
Impact of Periodontal Diseases on Oral Health-Related Quality of Life: A Study with a Condition-Specific Questionnaire in Croatian Population. 牙周病对口腔健康相关生活质量的影响:在克罗地亚人口中开展的一项针对具体病症的问卷调查研究。
Q1 Dentistry Pub Date : 2024-10-01 Epub Date: 2024-08-05 DOI: 10.1055/s-0044-1785534
Aleksandar Pupovac, Davor Kuiš, Ivana Mišković, Jelena Prpić

Objectives:  The aim of this study was to translate and validate the condition-specific Oral Health Impact Profile (OHIP) in the Croatian cultural context and assess the impact of periodontal diseases and nonsurgical periodontal therapy on quality of life (QoL).

Materials and methods:  A cross-sectional study was carried out on 150 individuals: 50 periodontally healthy, 50 with gingivitis, and 50 with periodontitis who self-administrated the OHIP. The participants' age ranged between 18 and 71 years, with the median age of 45 (34-57) years. Forty-seven percent of the participants were females. The validity and reliability of the Croatian OHIP version were tested. The impact of gingivitis and periodontitis on QoL was assessed. Changes in QoL induced by nonsurgical periodontal therapy in 20 patients with periodontitis were analyzed.

Statistical analysis:  Categorical data were presented by absolute and relative frequencies. The normality of the distribution of continuous variables was tested by the Shapiro-Wilk test. Continuous data were described by the median and the limits of the interquartile range (IQR). Differences in continuous variables between two independent groups were tested with the Mann-Whitney U test, and between three groups with the Kruskal-Wallis test (post hoc Conover). The Wilcoxon signed-rank test was used to examine the differences in the total score before and after therapy. All p values were two-sided. The level of significance was set at alpha of 0.05.

Results:  The analysis detected a single-factor structure that explained for the 56.9% of the variance. Cronbach's alpha value was 0.937, which indicated an excellent internal consistency. Overall OHIP score reported a strong correlation with the subjective estimate of periodontal problems (Rho = 0.92; p < 0.001). Test-retest reliability was high (r = 0.984; p < 0.001). The periodontitis group had the highest OHIP score (28 [23-34]), followed by the gingivitis group (14 [12-20]) and the periodontally healthy group (9 [5-11]; p < 0.001). Nonsurgical periodontal therapy significantly improved the QoL in those with periodontitis (p < 0.001).

Conclusion:  The condition-specific Croatian version of the OHIP instrument can be considered adequate to measure the impact of periodontal diseases on oral health-related QoL. Periodontal diseases, especially periodontitis, have a negative effect on the patient's QoL. Nonsurgical periodontal treatment can improve patients' QoL.

研究目的本研究的目的是在克罗地亚文化背景下翻译和验证特定条件下的口腔健康影响档案(OHIP),并评估牙周疾病和非手术牙周治疗对生活质量(QoL)的影响:对 150 人进行了横断面研究:材料:对 150 人进行了横断面研究:50 名牙周健康者、50 名牙龈炎患者和 50 名牙周炎患者,他们自行填写了 OHIP。参与者的年龄在 18 至 71 岁之间,年龄中位数为 45(34-57)岁。47%的参与者为女性。对克罗地亚 OHIP 版本的有效性和可靠性进行了测试。评估了牙龈炎和牙周炎对 QoL 的影响。统计分析:分类数据以绝对频率和相对频率表示。连续变量的分布正态性通过 Shapiro-Wilk 检验。连续数据用中位数和四分位数间距(IQR)表示。两个独立组间连续变量的差异用 Mann-Whitney U 检验,三个组间连续变量的差异用 Kruskal-Wallis 检验(事后 Conover)。Wilcoxon 符号秩检验用于检验治疗前后总分的差异。所有 p 值均为双侧。显著性水平设定为 0.05:分析结果显示,单因素结构解释了 56.9% 的方差。Cronbach's alpha 值为 0.937,显示出良好的内部一致性。OHIP 总分与牙周问题的主观估计值有很强的相关性(Rho = 0.92;p r = 0.984;p p p 结论:针对具体情况的克罗地亚版 OHIP 工具可用于衡量牙周疾病对口腔健康相关生活质量的影响。牙周疾病,尤其是牙周炎,对患者的 QoL 有负面影响。非手术牙周治疗可以改善患者的 QoL。
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引用次数: 0
Comparison of the Shear Bond Strength Using Primers with Different Application Numbers on Dental Zirconia. 在牙科氧化锆上使用不同涂敷次数的底漆的剪切粘接强度比较
Q1 Dentistry Pub Date : 2024-10-01 Epub Date: 2024-07-16 DOI: 10.1055/s-0043-1777821
Suphakit Opatragoon, Awiruth Klaisiri, Tool Sriamporn, Niyom Thamrongananskul

Objective:  This study examined the effect of the number of phosphate-containing primer applications on the shear bond strength (SBS) of zirconia to resin cement.

Materials and methods:  315 square specimens (10 × 10 × 4 mm3) were manufactured from Cercon ht presintered zirconia blocks. Alumina particles were used to sandblast zirconia specimens. These specimens were randomly divided into six primer-based groups: No primer application (NP), CLEARFIL CERAMIC PRIMER (C), PANAVIA V5 Tooth Primer (T), M&C PRIMER (MC), Monobond N (MN), and Z-PRIME plus (Z), and then separated into application number (1-4) groups (excluding NP). Each specimen was bonded with resin cement. The SBS was measured using a universal testing machine. The debonded surface was examined with a stereomicroscope.

Statistical analysis:  The SBSs were analyzed using two-way analysis of variance.

Results:  Applying the primer twice exhibited the highest SBSs in each group, with significant differences in the T, MN, and Z groups. However, the SBS in the MC group was significantly lower on the second application. One-hundred percent adhesive failure was observed in all groups.

Conclusion:  Within the limitations of this study, prior to cementation, the sandblasted zirconia surface should be applied twice with a phosphate-containing primer other than MC to maximize the SBS at the zirconia-resin cement interface.

目的:本研究探讨了使用含磷底漆的次数对氧化锆与树脂水泥的剪切结合强度(SBS)的影响:材料与方法:用 Cercon ht 预烧结氧化锆块制作了 315 个正方形试样(10 × 10 × 4 mm3)。氧化铝颗粒用于喷砂氧化锆试样。这些试样被随机分为六组:无底漆组 (NP)、CLEARFIL 陶瓷底漆组 (C)、PANAVIA V5 牙齿底漆组 (T)、M&C 底漆组 (MC)、Monobond N 底漆组 (MN) 和 Z-PRIME plus 底漆组 (Z),然后再分成 1-4 组(不包括 NP)。每个试样都用树脂水泥粘接。使用万能试验机测量 SBS。用体视显微镜检查脱粘表面:统计分析:采用双因素方差分析法分析 SBS:结果:在各组中,涂抹两次底漆的 SBS 值最高,T、MN 和 Z 组差异显著。然而,MC 组的 SBS 在第二次涂抹时明显降低。所有组别都出现了 100% 的粘合失败:在本研究的限制条件下,在骨水泥粘结前,喷砂氧化锆表面应涂抹两次除MC以外的含磷酸盐底漆,以最大限度地提高氧化锆-树脂骨水泥界面的SBS。
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引用次数: 0
The Potential of QP3VH-Chitosan Peptide as Biomimetic Remineralization in Early Dental Caries Treatment: An In Vitro Study. QP3VH-壳聚糖肽在早期龋齿治疗中的仿生再矿化潜力:体外研究
Q1 Dentistry Pub Date : 2024-10-01 Epub Date: 2024-05-17 DOI: 10.1055/s-0044-1782189
Prima Agusmawanti, Diatri Nari Ratih, Nunuk Purwanti, Tri Joko Raharjo

Objectives:  The development of remineralization biomimetics using organic peptide molecules is expected to resemble the hydroxyapatite (HA) mineralization process in tooth enamel. The development of an amelogenin derivative peptide combined with antimicrobial peptide was designed, resulting in QP3VH. This combination then was mixed with chitosan as a carrier. This study aimed to evaluate the biomimetic efficacy of QP3VH as a remineralizing agent combined with chitosan.

Materials and methods:  Fifty deciduous mandibular incisor enamel samples were used in this study. The artificial enamel lesions were created on a buccal surface and were randomly assigned to five groups of 10 each according to the remineralizing agent used: QP3VH, NaF, QP3VH + NaF, QP3VH + CS (QP3VH + chitosan), and saline distilled water (SDW). Each group was performed pH cycling for seven days. Enamel surface morphology and evaluation of mineral content Ca/P (calcium and phosphate) using scanning electron microscopy and energy dispersive X-ray analysis. The assessment was carried out, after demineralization, and after application with remineralization agents.

Statistical analysis:  Data were analyzed using a one-way analysis of variance followed by least significance difference post-hoc test. The paired t-test was utilized to compare the demineralization and remineralization results. The significance level used was 95%.

Results:  The remineralized group exhibited a significant increase in calcium and phosphate content on the enamel surface (p <0.05), and QP3VH + CS produced the maximum Ca/P mass percent after remineralization.

Conclusion:  Combining QP3VH with chitosan produces greatest remineralization than QP3VH, QP3VH + NaF, Naf, and SDW; therefore, QP3VH peptide has potential as a remineralizing agent, in the future.

目标:利用有机肽分子开发再矿化生物仿生学有望类似于牙釉质中的羟基磷灰石(HA)矿化过程。我们设计开发了一种结合了抗菌肽的amelogenin 衍生物肽,这就是 QP3VH。然后将这一组合与壳聚糖混合作为载体。本研究旨在评估 QP3VH 作为再矿化剂与壳聚糖结合的生物仿生功效:本研究使用了 50 个下颌切牙牙釉质样本。在颊面制作人工釉质病损,根据所使用的再矿化剂随机分配到五组,每组 10 个:QP3VH、NaF、QP3VH + NaF、QP3VH + CS(QP3VH + 壳聚糖)和生理盐水蒸馏水(SDW)。每组都进行了为期七天的 pH 循环。使用扫描电子显微镜和能量色散 X 射线分析评估珐琅质表面形态和矿物质含量 Ca/P(钙和磷酸盐)。评估在脱矿化后和使用再矿化剂后进行:数据分析采用单因素方差分析,然后进行显著性差异最小的事后检验。采用配对 t 检验比较脱矿和再矿化的结果。显著性水平为 95%:结果:再矿化组牙釉质表面的钙和磷酸盐含量显著增加(p 结论:再矿化组牙釉质表面的钙和磷酸盐含量显著增加:与 QP3VH、QP3VH + NaF、Naf 和 SDW 相比,将 QP3VH 与壳聚糖结合可产生最大的再矿化效果;因此,QP3VH 肽将来有可能成为一种再矿化剂。
{"title":"The Potential of QP3VH-Chitosan Peptide as Biomimetic Remineralization in Early Dental Caries Treatment: An In Vitro Study.","authors":"Prima Agusmawanti, Diatri Nari Ratih, Nunuk Purwanti, Tri Joko Raharjo","doi":"10.1055/s-0044-1782189","DOIUrl":"10.1055/s-0044-1782189","url":null,"abstract":"<p><strong>Objectives: </strong> The development of remineralization biomimetics using organic peptide molecules is expected to resemble the hydroxyapatite (HA) mineralization process in tooth enamel. The development of an amelogenin derivative peptide combined with antimicrobial peptide was designed, resulting in QP3VH. This combination then was mixed with chitosan as a carrier. This study aimed to evaluate the biomimetic efficacy of QP3VH as a remineralizing agent combined with chitosan.</p><p><strong>Materials and methods: </strong> Fifty deciduous mandibular incisor enamel samples were used in this study. The artificial enamel lesions were created on a buccal surface and were randomly assigned to five groups of 10 each according to the remineralizing agent used: QP3VH, NaF, QP3VH + NaF, QP3VH + CS (QP3VH + chitosan), and saline distilled water (SDW). Each group was performed pH cycling for seven days. Enamel surface morphology and evaluation of mineral content Ca/P (calcium and phosphate) using scanning electron microscopy and energy dispersive X-ray analysis. The assessment was carried out, after demineralization, and after application with remineralization agents.</p><p><strong>Statistical analysis: </strong> Data were analyzed using a one-way analysis of variance followed by least significance difference post-hoc test. The paired <i>t</i>-test was utilized to compare the demineralization and remineralization results. The significance level used was 95%.</p><p><strong>Results: </strong> The remineralized group exhibited a significant increase in calcium and phosphate content on the enamel surface (<i>p</i> <0.05), and QP3VH + CS produced the maximum Ca/P mass percent after remineralization.</p><p><strong>Conclusion: </strong> Combining QP3VH with chitosan produces greatest remineralization than QP3VH, QP3VH + NaF, Naf, and SDW; therefore, QP3VH peptide has potential as a remineralizing agent, in the future.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":"1149-1156"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11479723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Capsaicin on Migration and Alkaline Phosphatase Activity of Dental Pulp Cells. 辣椒素对牙髓细胞迁移和碱性磷酸酶活性的影响
Q1 Dentistry Pub Date : 2024-10-01 Epub Date: 2024-05-02 DOI: 10.1055/s-0044-1782191
Kittipot Khonglim, Boontharika Chuenjitkuntaworn, Yukihiko Tamura, Pornpoj Fuangtharnthip

Objectives:  Dental pulp, a specialized mesenchymal tissue within teeth, is pivotal in dental health and tissue repair. Capsaicin, the primary pungent component of chili peppers, is known for its diverse pharmacological properties. While capsaicin's effects on various cell types have been studied, its impact on dental pulp cells remains relatively unexplored. This study investigated the influence of pure capsaicin extract on dental pulp cell behavior, focusing on cell viability, proliferation, migration, and alkaline phosphatase (ALP) activity.

Materials and methods:  Capsaicin solution was prepared and diluted to various concentrations (1 nM, 0.01 µM, 0.1 µM, 1 µM, 10 µM, and 100 µM), then was tested on rat dental pulp cells (RPC-C2A). Cell viability and proliferation were assessed using the MTT assay. Boyden chamber tests and wound healing were used for evaluating cell migration. The activity of ALP was determined to show cell function during dental pulp repair.

Statistical analysis:  The data were analyzed using a one-way analysis of variance or an independent-sample Kruskal-Wallis, followed by multiple comparison tests.

Results:  Capsaicin of 100 µM exhibited cytotoxicity, whereas those with lower concentrations stimulated cell proliferation. Wound healing assays revealed increased cell migration, particularly when cultured with 1 nM capsaicin (p = 0.002). Boyden chamber assays demonstrated enhanced cell invasion without statistical significance. ALP activity of dental pulp cells increased significantly at 1 nM (p < 0.001) and 1 µM (p = 0.021) capsaicin concentrations, indicating potential dentinogenesis and pulp repair.

Conclusion:  Capsaicin of lower concentrations, less than 10 µM, is likely to promote proliferation, migration, and ALP activity of dental pulp cells. Our findings offer potential applications for capsaicin as a medication for dental pulp repair.

目的:牙髓是牙齿内一种特殊的间充质组织,在牙齿健康和组织修复方面起着关键作用。辣椒素是辣椒的主要辛辣成分,具有多种药理特性。虽然人们已经研究了辣椒素对各种细胞类型的影响,但其对牙髓细胞的影响仍相对较少。本研究调查了纯辣椒素提取物对牙髓细胞行为的影响,重点关注细胞活力、增殖、迁移和碱性磷酸酶(ALP)活性:制备辣椒素溶液并稀释至不同浓度(1 nM、0.01 µM、0.1 µM、1 µM、10 µM和100 µM),然后在大鼠牙髓细胞(RPC-C2A)上进行测试。细胞活力和增殖采用 MTT 法进行评估。波登室试验和伤口愈合用于评估细胞迁移。测定 ALP 的活性以显示牙髓修复过程中的细胞功能:数据采用单因素方差分析或独立样本 Kruskal-Wallis 分析,然后进行多重比较试验:结果:100 µM的辣椒素具有细胞毒性,而较低浓度的辣椒素能刺激细胞增殖。伤口愈合试验显示细胞迁移增加,尤其是用 1 nM 的辣椒素培养时(p = 0.002)。波登室试验显示细胞侵袭能力增强,但无统计学意义。牙髓细胞的 ALP 活性在 1 nM(p = 0.021)的辣椒素浓度下显著增加,表明潜在的牙本质生成和牙髓修复:结论:低于 10 µM 的低浓度辣椒素可能会促进牙髓细胞的增殖、迁移和 ALP 活性。我们的研究结果为辣椒素作为牙髓修复药物提供了潜在的应用前景。
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引用次数: 0
Analysis of Stress Distribution and Displacement Based on the Miniscrew Positions of the Palatal Slope Bone-borne Expander: A Finite Element Study. 基于腭坡骨性扩张器微型螺钉位置的应力分布和位移分析:有限元研究。
Q1 Dentistry Pub Date : 2024-10-01 Epub Date: 2024-03-31 DOI: 10.1055/s-0043-1777823
Chakree Leeisaramas, Nattapon Chantarapanich, Samroeng Inglam, Kanlaya Insee

Objectives:  This study aimed to investigate the stress distribution pattern of the palatal slope bone-borne expander on the maxillary area according to a different anteroposterior position of anchored miniscrews using finite element analysis.

Materials and methods:  Nasomaxillary stereolithography files with three different anteroposterior anchored miniscrew positions of the palatal slope bone-borne expander were determined as model A, B, and C. Each model consists of four supported miniscrews. Model A: two anterior miniscrews were located between the maxillary canine and the first premolar, and two posteriors between the second premolar and the first molar. Model B: two anteriors were between the lateral incisor and the canine, and two posteriors were the same as in model A. Model C: two anteriors were the same as in model A, and two posteriors were distal to the first molar. One turn of expander screws was applied. Maximum principal stress, equivalent elastic strain, equivalent von Mises stress, and transverse displacement were evaluated.

Results:  The maximum principal stress was mostly found at the bone-miniscrew interface. Model A exhibited an intersecting area of stress between the supported miniscrews. The highest value of principal stress was in model B, while model C showed a uniform distribution pattern. The elastic strain pattern was similar to the principal stress in all models. The highest value of equivalent von Mises stress was located on the expander screw. The largest amount of transverse displacement of teeth was in model A, while model C exhibited a more consistent transverse displacement than other models. Vertical displacement of posterior teeth was also noticed.

Conclusion:  Based on the result, it revealed that the various anteroposterior miniscrew placements of the palatal slope bone-borne expander had various patterns of stress distribution and resulted in various outcomes. It may be inferred that model A's miniscrew location was advantageous for obtaining expansion quantities, but model C's miniscrew position was advantageous for maintaining consistent biomechanics.

研究目的本研究旨在利用有限元分析方法,研究腭坡骨性扩张器在不同的锚定微型螺钉前胸位置下,上颌区域的应力分布模式:确定了腭斜骨性扩张器的三种不同前胸锚定微型螺钉位置的鼻颌立体光刻文件为模型 A、B 和 C,每个模型由四个支撑微型螺钉组成。模型 A:两个前方迷你螺钉位于上颌犬齿和第一前磨牙之间,两个后方迷你螺钉位于第二前磨牙和第一臼齿之间。模型 B:两颗前牙位于侧切牙和犬齿之间,两颗后牙与模型 A 相同。模型 C:两颗前牙与模型 A 相同,两颗后牙位于第一磨牙远端。使用一圈扩张螺钉。对最大主应力、等效弹性应变、等效 von Mises 应力和横向位移进行了评估:结果:最大主应力主要出现在骨-微型螺钉界面。模型 A 在支撑的微型螺钉之间出现了应力交叉区。主应力的最高值出现在模型 B 中,而模型 C 则呈现出均匀分布的模式。所有模型的弹性应变模式都与主应力相似。等效 von Mises 应力的最高值位于胀紧螺钉上。模型 A 的牙齿横向位移量最大,而模型 C 的横向位移量比其他模型更稳定。后牙的垂直位移也被注意到:根据结果显示,腭侧斜坡骨性扩张器的各种前后微型螺钉位置具有不同的应力分布模式,并导致不同的结果。可以推断,模型 A 的微型螺钉位置有利于获得扩张量,而模型 C 的微型螺钉位置则有利于保持生物力学的一致性。
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引用次数: 0
Anticancer Potential of Quercetin on Oral Squamous Cell Carcinoma: A Scoping Review and Molecular Docking. 槲皮素对口腔鳞状细胞癌的抗癌潜力:范围综述和分子对接。
Q1 Dentistry Pub Date : 2024-09-30 DOI: 10.1055/s-0044-1789016
Dhona Afriza, Utmi Arma, Raefany Faslah, Wastuti Hidayati Suriyah

Oral squamous cell carcinoma (OSCC) is a type of cancer that has a low survival rate and high recurrence and metastasis rates. To date, there is still no effective treatment for OSCC. Various types of cancer, including OSCC, have reported quercetin to act as an anticancer agent, but there is no clear research data on how it may affect OSCC. To determine the anticancer potential of quercetin in OSCC, we conducted a scoping review, and to determine the interaction of quercetin with one of the proteins that plays a role in carcinogenesis, namely, BCL-2, we conducted molecular docking. The scoping review process was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. The scoping review was searched by collecting articles related to the research topic in Google Scholar, PubMed, ScienceDirect, Cochrane, and EBSCOhost databases. All of the literature records found during the search were imported into the Mendeley software to remove duplication. Nine studies were generated after the titles and abstracts were reviewed according to the inclusion and exclusion criteria. After the full-text screening, no studies were excluded, leaving nine publications determined to be eligible for inclusion in the scoping review. Quercetin showed effects on inhibiting cancer invasion, migration, proliferation, and many protein expressions, as well as increasing cell apoptosis. Molecular docking was done for quercetin and BCl-2 protein. Doxorubicin was utilized as a comparison ligand. The in silico study was utilized using AutoDock Vina, AutoDock Tools 1.5.6, Biovia Discovery Studio 2021, and PyMol. Molecular docking indicated quercetin has a strong binding affinity with BCl-2 protein (ΔG -7.2 kcal/mol). Both scoping review and molecular docking revealed that quercetin is a promising candidate for anticancer agent.

口腔鳞状细胞癌(OSCC)是一种生存率低、复发和转移率高的癌症。迄今为止,OSCC 仍然没有有效的治疗方法。据报道,槲皮素可作为一种抗癌剂作用于包括 OSCC 在内的多种癌症,但关于槲皮素如何影响 OSCC,目前尚无明确的研究数据。为了确定槲皮素在OSCC中的抗癌潜力,我们进行了一次范围审查,为了确定槲皮素与一种在致癌过程中起作用的蛋白质(即BCL-2)之间的相互作用,我们进行了分子对接。范围界定综述过程是根据《系统综述和元分析的首选报告项目》(Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews)进行的。通过在谷歌学术、PubMed、ScienceDirect、Cochrane 和 EBSCOhost 数据库中收集与研究主题相关的文章,对范围界定综述进行了检索。搜索过程中发现的所有文献记录都被导入到 Mendeley 软件中,以去除重复内容。根据纳入和排除标准对标题和摘要进行审查后,产生了 9 篇研究报告。经过全文筛选,没有研究被排除在外,因此确定有九篇出版物符合纳入范围界定审查的条件。槲皮素具有抑制癌症侵袭、迁移、增殖和多种蛋白质表达以及增加细胞凋亡的作用。槲皮素与 BCl-2 蛋白进行了分子对接。多柔比星被用作对比配体。硅学研究使用了 AutoDock Vina、AutoDock Tools 1.5.6、Biovia Discovery Studio 2021 和 PyMol。分子对接表明,槲皮素与 BCl-2 蛋白具有很强的结合亲和力(ΔG -7.2 kcal/mol)。范围综述和分子对接均表明,槲皮素是一种很有希望的候选抗癌剂。
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引用次数: 0
Variability in Positions and Factors Contributing to Surgical Difficulty of Impacted Third Molars. 影响第三磨牙手术难度的位置变化和因素。
Q1 Dentistry Pub Date : 2024-09-27 DOI: 10.1055/s-0044-1788796
Endang Sjamsudin, Anggun Rafisa, Nuroh Najmi

Objectives:  This study aimed to provide valuable insights into the variability of third molar positions and factors influencing their surgical time and technique.

Materials and methods:  This cross-sectional study included a total of 48 eligible participants, aged 18 to 45 years, diagnosed with impacted teeth, and who had undergone surgery. Exclusion criteria comprised the absence of the second molar, the presence of systemic diseases, a history of radiation therapy, and pregnancy or lactation. Participants completed a questionnaire covering demographic data, physical metrics, and information on systemic conditions and disorders. Preoperative assessments included vital sign measurements. Panoramic imaging was employed to evaluate the third molar distance to the occlusal plane, degrees of angulation, eruption space, and distance to the alveolar inferior canal. Surgical difficulty in this study was measured by two outcomes: surgical time and technique.

Statistical analysis:  To assess differences in impacted third molar positions among groups, Independent samples t-test and One-way analysis of variance were used for normally distributed data without outliers; otherwise, the Mann-Whitney U test and Kruskal-Wallis H test were utilized. The Spearman's rank correlation was utilized to explore relationships between vital signs, impacted third molar positions, surgical time, and surgical technique.

Results:  There were no significant differences in third molar positions between age and body mass index groups. Significant differences in distance to the occlusal plane were observed between third molars in quadrants 2 and 3 (p = 0.002) and quadrants 2 and 4 (p = 0.005). A significant difference in eruption space was found between sexes (p = 0.016). A significant negative correlation was discovered between surgical time and respiration rate per minute (p = 0.028).

Conclusion:  This study found that males have greater third molar eruption space than females, and maxillary third molars have a greater distance to the occlusal plane compared with mandibular third molars. The importance of vital signs as contributing factors to surgical difficulty is highlighted, emphasizing their relevance in clinical practice.

研究目的本研究旨在就第三磨牙位置的变化以及影响其手术时间和技术的因素提供有价值的见解:这项横断面研究共纳入了 48 名符合条件的参与者,他们的年龄在 18 至 45 岁之间,被诊断患有阻生牙并接受过手术。排除标准包括没有第二磨牙、患有全身性疾病、有放射治疗史、怀孕或哺乳期。受试者填写了一份调查问卷,内容包括人口统计学数据、身体指标以及全身状况和疾病信息。术前评估包括生命体征测量。全景成像用于评估第三磨牙与咬合面的距离、成角程度、萌出空间以及与牙槽下管的距离。本研究中的手术难度通过两个结果来衡量:手术时间和技术:为评估各组间第三磨牙撞击位置的差异,对无异常值的正态分布数据采用独立样本 t 检验和单因素方差分析,否则采用 Mann-Whitney U 检验和 Kruskal-Wallis H 检验。利用斯皮尔曼等级相关性来探讨生命体征、受影响的第三磨牙位置、手术时间和手术技术之间的关系:结果:不同年龄组和体重指数组的第三磨牙位置无明显差异。第二象限和第三象限(P = 0.002)以及第二象限和第四象限(P = 0.005)的第三磨牙与咬合平面的距离存在显著差异。性别间的萌出空间存在明显差异(p = 0.016)。手术时间与每分钟呼吸频率呈明显负相关(p = 0.028):本研究发现,男性的第三磨牙萌出间隙大于女性,上颌第三磨牙与咬合面的距离大于下颌第三磨牙。生命体征是导致手术困难的重要因素,强调了生命体征在临床实践中的重要性。
{"title":"Variability in Positions and Factors Contributing to Surgical Difficulty of Impacted Third Molars.","authors":"Endang Sjamsudin, Anggun Rafisa, Nuroh Najmi","doi":"10.1055/s-0044-1788796","DOIUrl":"https://doi.org/10.1055/s-0044-1788796","url":null,"abstract":"<p><strong>Objectives: </strong> This study aimed to provide valuable insights into the variability of third molar positions and factors influencing their surgical time and technique.</p><p><strong>Materials and methods: </strong> This cross-sectional study included a total of 48 eligible participants, aged 18 to 45 years, diagnosed with impacted teeth, and who had undergone surgery. Exclusion criteria comprised the absence of the second molar, the presence of systemic diseases, a history of radiation therapy, and pregnancy or lactation. Participants completed a questionnaire covering demographic data, physical metrics, and information on systemic conditions and disorders. Preoperative assessments included vital sign measurements. Panoramic imaging was employed to evaluate the third molar distance to the occlusal plane, degrees of angulation, eruption space, and distance to the alveolar inferior canal. Surgical difficulty in this study was measured by two outcomes: surgical time and technique.</p><p><strong>Statistical analysis: </strong> To assess differences in impacted third molar positions among groups, Independent samples <i>t</i>-test and One-way analysis of variance were used for normally distributed data without outliers; otherwise, the Mann-Whitney U test and Kruskal-Wallis H test were utilized. The Spearman's rank correlation was utilized to explore relationships between vital signs, impacted third molar positions, surgical time, and surgical technique.</p><p><strong>Results: </strong> There were no significant differences in third molar positions between age and body mass index groups. Significant differences in distance to the occlusal plane were observed between third molars in quadrants 2 and 3 (<i>p</i> = 0.002) and quadrants 2 and 4 (<i>p</i> = 0.005). A significant difference in eruption space was found between sexes (<i>p</i> = 0.016). A significant negative correlation was discovered between surgical time and respiration rate per minute (<i>p</i> = 0.028).</p><p><strong>Conclusion: </strong> This study found that males have greater third molar eruption space than females, and maxillary third molars have a greater distance to the occlusal plane compared with mandibular third molars. The importance of vital signs as contributing factors to surgical difficulty is highlighted, emphasizing their relevance in clinical practice.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and Characterization of Key Genes Associated with Amelogenesis. 与髓鞘形成有关的关键基因的鉴定和特征描述。
Q1 Dentistry Pub Date : 2024-09-19 DOI: 10.1055/s-0044-1787958
Tahsinul Haque, Fatema Akhter, Nourelhoda Alim, Abdullah Nabhan, Fawzia Al Kahtani, Abdullah Mohammed Sambawa

Objectives:  The identification of key genes associated with amelogenesis would be helpful in finding solutions to genetic disorders in oral biology. The study aimed to use in silico analysis to identify the key genes involved in tooth development associated with preameloblasts (PABs) and secretory ameloblasts (SABs).

Material and methods:  The data was subjected to quality analysis and uniform manifold approximation and projection analysis. To examine the distribution of the genes and identify important upregulated loci, a p-value histogram, a quantile plot, a mean difference and mean-variance plot, and a volcano plot were generated. Finally, protein-protein interaction and gene enrichment analyses were performed to determine the ontology, relevant biological processes, and molecular functions of selected genes.

Results:  A total of 157 genes were found to be significant in the PAB versus SAB comparison. HIST1H31 revealed strong interaction with HIST1H2BM, and EXO1, ASPM, SPC25, and TTK showed strong interactions with one other. The STRING database revealed that NCAPG, CENPU, NUSAP1, HIST1H2BM, and HIST1H31 are involved in biological processes. NCAPG, CENPU, SPC25, ETV5, TTK, ETV1, FAM9A, NUSAP1, HIST1H2BM, and HIST1H31 are involved in cellular components.

Conclusion:  The TTK, NUSAP1, CENPU, NCAPG, FAM9A, ASPM, SPC25, and HIST1H31 genes demonstrate functions in cell division. These genes might play a role in ameloblast development. These results will be useful in developing new methods to stimulate ameloblast development, which is essential for tooth regeneration and tissue engineering. However, more research is required to validate the functions of these genes and the genes with which they interact. A wide variety of genetic, epigenetic, and exogenous signaling factors regulate these genes and pathways throughout development and differentiation, cell fate, and behavior.

目的:确定与釉质形成相关的关键基因将有助于找到解决口腔生物学遗传疾病的方法。本研究旨在利用硅学分析鉴定与成釉细胞(PABs)和分泌性成釉细胞(SABs)相关的牙齿发育的关键基因:对数据进行了质量分析、统一流形近似和投影分析。为了研究基因的分布并确定重要的上调位点,生成了 p 值直方图、量子图、均值差和均值方差图以及火山图。最后,还进行了蛋白质-蛋白质相互作用和基因富集分析,以确定所选基因的本体、相关生物学过程和分子功能:结果:共发现 157 个基因在 PAB 与 SAB 比较中具有显著性。HIST1H31显示与HIST1H2BM有很强的相互作用,EXO1、ASPM、SPC25和TTK显示彼此有很强的相互作用。STRING 数据库显示,NCAPG、CENPU、NUSAP1、HIST1H2BM 和 HIST1H31 参与了生物过程。NCAPG、CENPU、SPC25、ETV5、TTK、ETV1、FAM9A、NUSAP1、HIST1H2BM 和 HIST1H31 参与了细胞成分:结论:TTK、NUSAP1、CENPU、NCAPG、FAM9A、ASPM、SPC25 和 HIST1H31 基因具有细胞分裂功能。这些基因可能在成髓细胞的发育过程中发挥作用。这些结果将有助于开发刺激牙釉质母细胞发育的新方法,而牙釉质母细胞发育对牙齿再生和组织工程至关重要。然而,还需要更多的研究来验证这些基因的功能及其相互作用的基因。在整个发育和分化、细胞命运和行为过程中,有各种各样的遗传、表观遗传和外源信号因子对这些基因和通路进行调控。
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引用次数: 0
期刊
European Journal of Dentistry
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