Food and Bioproducts Processing最新文献_第10页

Structural diversity of monosaccharides dictates emulsification functionality: Role of casein-glycation conjugates fabricated via wet-heating reaction 单糖的结构多样性决定了乳化功能：通过湿加热反应制备的酪蛋白糖化偶联物的作用

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-11-07 DOI: 10.1016/j.fbp.2025.11.002

Lingfan Zhao , Xia Lu , Haowen Luo , Qing Wang , Qinyi Gao , Xianglin Zhang , Xingrun Wang , Qiqi Li , Wupeng Ge , Shuangshuang Wang

Monosaccharides bearing specific functional groups vary in their reactivity during wet-heating Maillard reaction（MR） conjugation with casein (CN), leading to conjugates with distinct physicochemical properties. In this study, CN was conjugated with D-tagatose (DT) or N-acetylglucosamine (GlcNAc) via a wet-heating MR to prepare two water-soluble conjugates: DT:CN (D:C) and GlcNAc:CN (G:C). Systematically investigating the characterize properties and structures of these conjugates and emulsion stabilization mechanisms. The conjugates achieved grafting degrees of 20.0 % (D:C) and 19.7 % (G:C) under optimized MR conditions, with SDS-PAGE and FT-IR confirming covalent macromolecular conjugation. Additionally, circular dichroism (CD) analysis revealed a remarkable enhancement in α-helix content to about 1.6 times that of native CN, accompanied by 6–10°C increases in denaturation temperature (T_d), demonstrating thermally stabilized architectures. Structural modifications induced significant physicochemical improvements: particle size reduces by nearly half, Zeta-potential significantly increases, and surface hydrophobicity reduced by 72.3 % (D:C) and 34.5 % (G:C). Scanning electron microscopy further revealed optimized film-forming capabilities in conjugates. As emulsifiers, the modified proteins reduced emulsion droplet sizes by over 50 % while increasing surface charges by 53.4 % (D:C) and 12.2 % (G:C), respectively, indicating superior emulsification performance. Thus, the information obtained in this study can enrich the theoretical framework of the interaction between proteins and different monosaccharides structures.

带有特定官能团的单糖在与酪蛋白（CN）的湿加热美拉德反应（MR）偶联过程中，其反应活性会发生变化，从而产生具有不同物理化学性质的偶联物。本研究通过湿加热MR将CN与D-塔格糖（DT）或n -乙酰氨基葡萄糖（GlcNAc）偶联，制备了DT:CN （D:C）和GlcNAc:CN （G:C）两种水溶性偶联物。系统地研究了这些共轭物的性质、结构和乳液稳定机理。在优化的MR条件下，共轭物的接枝度分别为20.0 % （D:C）和19.7 % (G:C)， SDS-PAGE和FT-IR证实了共价大分子的共轭。此外，圆二色性（CD）分析显示，α-螺旋含量显著增加，约为天然CN的1.6倍，同时变性温度（Td）升高6-10℃，表现出热稳定的结构。结构修饰导致了显著的物理化学改善：颗粒尺寸减小了近一半，zeta电位显著增加，表面疏水性降低了72.3 % （D:C）和34.5% % （G:C）。扫描电镜进一步揭示了优化的共轭物成膜能力。作为乳化剂，改性蛋白使乳滴尺寸减小了50% %以上，表面电荷分别增加了53.4 % （D:C）和12.2 % (G:C)，显示出优异的乳化性能。因此，本研究获得的信息可以丰富蛋白质与不同单糖结构相互作用的理论框架。

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引用次数: 0

Prediction of major pressure losses pumping non-Newtonian foods and beverages – The role of uncertainty propagation for power law fluids 非牛顿食品和饮料的主要压力损失预测。幂律流体的不确定度传播的作用

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-10-24 DOI: 10.1016/j.fbp.2025.10.017

Andreas Håkansson , Hanna Ekelund , Hanna Isendahl , Hannah Viklund , Helena Wijkander , Dragana Arlov

To predict major pressure losses from pumping shear thinning liquid foods and beverage, rheometer-measured flow behaviour (n) and flow consistency (K) indices are needed. These are always obtained with some measurement uncertainty. This contribution develops a tool that allows for quantitatively estimating how a given measurement uncertainty in the rheological parameters translate to prediction uncertainty in major pressure losses, and how much measurement uncertainty can be allowed while ensuing that the prediction uncertainty is within a given tolerance. Methodologically, the study combines the general uncertainty management framework, Monte Carlo simulations, and an artificial neural network regression to predict uncertainty propagation. Experimental data on a range of liquid foods and beverages are used. Results show that relative measurement uncertainties of ∼2–5 % in n and K results in a prediction uncertainty of less than 10 % in the major pressure losses. Moreover, uncertainty in n influences the prediction quality more severely than uncertainty in K, and prediction uncertainty is larger the closer n is to 1. This is the first contribution providing a quantitative relationship between measurement uncertainty and prediction uncertainty for this application. It provides measurement guidelines and helps identify the role of uncertainty propagation in predicting pressure losses when pumping complex liquid foods.

为了预测泵送剪切稀释液态食品和饮料的主要压力损失，需要流变仪测量的流动特性(n)和流动一致性(K)指标。这些都是有一定测量不确定度的。这一贡献开发了一种工具，可以定量地估计流变参数中给定的测量不确定度如何转化为主要压力损失的预测不确定度，以及在预测不确定度在给定公差范围内时允许的测量不确定度。在方法上，该研究结合了一般的不确定性管理框架、蒙特卡罗模拟和人工神经网络回归来预测不确定性的传播。使用了一系列液态食品和饮料的实验数据。结果表明，n和K的相对测量不确定度为~ 2-5 %，导致主要压力损失的预测不确定度小于10 %。而且，n的不确定性比K的不确定性对预测质量的影响更大，且n越接近1，预测的不确定性越大。这是为该应用提供测量不确定度和预测不确定度之间定量关系的第一个贡献。它提供了测量指南，并有助于确定不确定度传播在泵送复杂液体食品时预测压力损失的作用。

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引用次数: 0

Green pathways to seaweed protein: a comparative techno-economic appraisal of pH shifting, MAE, UAE, and EAE 海藻蛋白的绿色途径：pH变化，MAE， UAE和EAE的比较技术经济评价

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-10-30 DOI: 10.1016/j.fbp.2025.10.021

Michael Edgardo Pérez-Roa, Loveille Jun Gonzaga, Roberto Lavecchia, Antonio Zuorro

Seaweeds are emerging as sustainable protein sources, yet large-scale extraction remains limited by high costs, low yields, and energy-intensive processing. This study presents a techno-economic and simplified environmental assessment of four green extraction routes for seaweed-protein recovery: pH shifting, microwave-assisted extraction, ultrasound-assisted extraction, and enzyme-assisted extraction, along with a hybrid pH-shifting + ultrasound configuration. Process models were developed from peer-reviewed data and current market information for Ulva biomass. Capital and operating costs, profitability indicators, energy use, and carbon emissions were evaluated under different product prices, protein recoveries, and drying options. Microwave-assisted extraction achieved the highest recovery (50 %) and lowest carbon intensity (10.8 kg CO₂ per kg protein). Drying dominated both cost and energy demand, representing more than two-thirds of total investment and electricity use. Replacing freeze- with oven-drying improved financial performance but increased emissions, while higher protein recovery enhanced both economic and environmental outcomes. The hybrid pH–ultrasound route offered a balanced compromise between efficiency and simplicity. This comparative appraisal provides the first integrated assessment of economic and environmental trade-offs across green extraction pathways, offering strategic insights for scaling seaweed protein production sustainably.

Tweetable abstract

Microwave-assisted and hybrid pH-shifting + ultrasound methods achieve the best balance between cost, efficiency, and environmental impact for seaweed-protein extraction.

海藻正在成为可持续的蛋白质来源，但大规模提取仍然受到高成本、低产量和能源密集型加工的限制。本研究提出了四种绿色海藻蛋白提取途径的技术经济和简化环境评估：pH转移、微波辅助提取、超声辅助提取和酶辅助提取，以及pH转移+ 超声混合配置。过程模型是根据同行评审的数据和Ulva生物质的当前市场信息开发的。在不同的产品价格、蛋白质回收率和干燥选项下，评估了资本和运营成本、盈利指标、能源使用和碳排放。微波辅助提取的回收率最高（50 %），碳强度最低（每公斤蛋白质10.8 kg CO₂）。干燥在成本和能源需求方面占主导地位，占总投资和用电量的三分之二以上。用烘箱干燥法取代冷冻法改善了财务绩效，但增加了排放，而更高的蛋白质回收率提高了经济和环境效益。混合ph -超声路线提供了效率和简单性之间的平衡折衷。这一比较评估首次对绿色提取途径的经济和环境权衡进行了综合评估，为可持续地扩大海藻蛋白生产规模提供了战略见解。摘要微波辅助和混合ph位移+ 超声方法在海藻蛋白提取的成本、效率和环境影响之间取得了最佳平衡。

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引用次数: 0

Dielectric properties of black soldier fly larvae at 2.45 GHz, and their dependence with moisture content, temperature, or bulk density 黑兵蝇幼虫在2.45 GHz下的介电特性及其与含水量、温度和容重的关系

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-10-25 DOI: 10.1016/j.fbp.2025.10.013

J. Fayos-Fernández , R. Pérez-Campos , J. Monzó-Cabrera , M. Nowakowski , J. Majerska , A. Zimmer

As the global population continues to rise, the demand for traditional food sources, especially animal protein, intensifies, leading to increasing scarcity. The black soldier fly (BSF) larvae are an alternative high protein source (up to 44 %) which farming is environmentally sustainable. This contribution aims to increase the industrial processing efficiency by examining the feasibility of using microwave technology at the right larvae instar for its storage. Microwave processing offers advantages such as rapid water removal, nutritional preservation, and microbial reduction. This paper characterizes experimentally the dielectric constant (ε′) and loss factor (ε′′) of BSF larvae, and their dependency with temperature, moisture content (MC), and bulk density (BD), to enable optimized microwave drying systems. Results show that the permittivity varies significantly with the MC. The decrease of MC, the goal of any drying process, is linked to the BD reduction, and this physical parameter has even a stronger influence on the permittivity. Finally, the temperature increase affects the loss factor, causing an increase of the heating speed. Nevertheless, all these magnitudes combined keeps the larvae dielectric parameters within a range that provides insights for improving the microwave drying efficiency of processed BSF larvae at an industrial scale.

随着全球人口的持续增长，对传统食物来源，特别是动物蛋白的需求增加，导致日益稀缺。黑兵蝇（BSF）幼虫是另一种高蛋白来源（高达44% %），养殖在环境上是可持续的。本论文旨在通过研究微波技术在合适的幼虫龄期进行储存的可行性，提高工业加工效率。微波处理具有快速除水、营养保存和微生物减少等优点。为了优化微波干燥系统，本文通过实验研究BSF幼虫介电常数（ε′）和损耗因子（ε′）及其与温度、含水量（MC）和容重（BD）的关系。结果表明，介质介质的介电常数随介质介质密度的变化有显著的变化，任何干燥过程的目标都是降低介质介质密度，而介质介质密度对介电常数的影响更大。最后，温度的升高影响了损耗因子，导致加热速度的增加。然而，所有这些数值结合起来，使幼虫的介电参数保持在一个范围内，这为提高工业规模上加工过的BSF幼虫的微波干燥效率提供了见解。

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引用次数: 0

Macrocystis pyrifera in the circular economy: Advances in cultivation, processing, and industrial integration 循环经济中的大囊藻：栽培、加工与产业整合进展

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-09-29 DOI: 10.1016/j.fbp.2025.09.020

Mia Gaiero , Jasmin S. Yang , Neill J. Goosen , Juliana M.L.N. de Moura Bell

The escalating pressure on existing production systems due to an increasing world population and limited availability of arable land, compounded by increasing atmospheric carbon dioxide concentration, has prompted the search for sustainable sources of macro- and micronutrients for use in industrial applications. Macroalgae or seaweeds are macroscopic marine algae that have been traditionally consumed in Asian countries for centuries. Because of their composition, wide availability, and growth-associated environmental benefits (i.e., carbon uptake, support to fishery habitats, and reduced dependence on fresh water, arable land, and fertilizer needs), macroalgae have been highlighted as a potentially sustainable biomass feedstock for the production of a wide range of polysaccharides (i.e., laminarin, fucoidans, alginate), growth hormones, proteins, and phenolic compounds (phlorotannins) which could potentially be used in human nutrition, animal feed, production of bio-stimulants or fertilizers for plant growth, biotherapeutic, cosmetic, and food applications. However, full utilization of macroalgae’s potential relies on the development of bio-guided processing strategies that are able to not only maximize the extractability of the diverse compounds, but also preserve the functional and biological properties of the extracts. This review highlights the use of brown macroalgae, focusing on the giant kelp Macrocystis pyrifera when possible, as a feedstock to produce added-value compounds. The scope encompasses sea farming, harvesting systems, and downstream processing conditions, with emphasis on innovative attempts to develop structure/function-based extraction methods to produce components with desired properties.

由于世界人口的增加和可耕地的有限，加上大气中二氧化碳浓度的增加，对现有生产系统的压力越来越大，促使人们寻找可持续的宏量和微量营养素来源，以供工业应用。大藻或海藻是一种宏观的海洋藻类，在亚洲国家已经被传统地食用了几个世纪。由于它们的组成、广泛的可获得性和与生长相关的环境效益（即碳吸收、支持渔业生境、减少对淡水、耕地和肥料需求的依赖），大型藻类已被强调为一种潜在的可持续生物质原料，用于生产各种多糖（即层粘胶蛋白、岩藻酸酯、藻酸盐）、生长激素、蛋白质、酚类化合物（苯酞酸）可能用于人类营养、动物饲料、生产生物刺激剂或植物生长肥料、生物治疗、化妆品和食品应用。然而，充分利用巨藻的潜力依赖于生物导向加工策略的发展，这些策略不仅能够最大限度地提高各种化合物的可提取性，而且还能保持提取物的功能和生物学特性。本文重点介绍了大型褐藻的应用，并在可能的情况下重点介绍了巨型海带巨藻（Macrocystis pyrifera）作为生产高附加值化合物的原料。范围包括海洋养殖、收获系统和下游加工条件，重点是创新尝试开发基于结构/功能的提取方法，以生产具有所需性能的组件。

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引用次数: 0

Biodegradable cryogels based on pea starch as carriers of bioactive compounds from pecan (Carya illinoinensis) co-product: Synthesis, characterization, and prospecting for technological use 以豌豆淀粉为载体的山核桃副产物生物活性化合物的可生物降解冷冻材料：合成、表征及技术应用前景

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-10-15 DOI: 10.1016/j.fbp.2025.10.014

Eduarda Caetano Peixoto , Elder Pacheco da Cruz , Tatiane Jéssica Siebeneichler , Laura Martins Fonseca , Julia Eisenhardt de Mello , Roselia Maria Spanevello , Alvaro Renato Guerra Dias , Elessandra da Rosa Zavareze , Cesar Valmor Rombaldi

Pecan nut shells are typically discarded as waste. However, their valorization holds significant potential due to the presence of phenolic compounds and antioxidant properties. This study sought to enhance the value of this byproduct by developing cryogels derived from pea starch, enriched with pecan nut shell extract (PNSE). Develop a fully biodegradable material suitable for food packaging that is capable of incorporating bioactive compounds. The cryogels were prepared by gelatinizing the starch, followed by physical crosslinking through five cycles of freezing and thawing. PNSE was incorporated into the cryogels at 10 % and 20 % (w/w). PNSE was evaluated for its individual phenolic compounds and cytotoxicity. The cryogels were assessed for their morphology, density, porosity, water absorption, hardness, antioxidant activity, reuse potential, and biodegradability. PNSE contained phenolic compounds, with tannins and procyanidins identified as the major groups. These compounds are primarily responsible for the bioactivity of PNSE. The cryogels exhibited a macroporous and homogeneous morphology. All cryogels had a low density (0.058–0.078 g/cm³), high porosity (88–91 %), and high water absorption, reaching up to 1513 %. The incorporation of PNSE increased the hardness of the cryogels, thereby enhancing their structural integrity. Cryogels demonstrated reusability over 15 cycles, with 600 % absorption potential. All cryogels were biodegradable in soil within 15 days. These cryogels represent effective matrices with potential applications as water absorbers for fresh food products that release exudates, as well as carriers of antioxidant and bioactive compounds.

山核桃壳通常被当作废物丢弃。然而，由于酚类化合物和抗氧化性能的存在，它们的增值具有显著的潜力。本研究试图通过开发富含核桃壳提取物（PNSE）的豌豆淀粉衍生的冷冻产品来提高这一副产品的价值。开发一种完全可生物降解的材料，适合于食品包装，能够纳入生物活性化合物。通过将淀粉糊化，然后经过5次冷冻和解冻的物理交联，制备出冷冻材料。PNSE分别以10 %和20 % （w/w）加入到冷冻液中。评估了PNSE的单个酚类化合物和细胞毒性。研究人员评估了这些冷冻液的形态、密度、孔隙率、吸水性、硬度、抗氧化活性、再利用潜力和生物降解性。PNSE含有酚类化合物，以单宁和原花青素为主要基团。这些化合物是PNSE生物活性的主要原因。所制备的冷库具有均匀的大孔结构。所有冷冻液均具有低密度（0.058 ~ 0.078 g/cm³）、高孔隙率（88 ~ 91 %）和高吸水性（高达1513 %）的特点。PNSE的加入提高了低温材料的硬度，从而增强了其结构完整性。制冷机可重复使用超过15次循环，具有600 %的吸收潜力。所有冷冻剂在15天内均可在土壤中降解。这些冷藏箱代表了有效的基质，具有潜在的应用价值，可以作为新鲜食品中释放渗出物的吸水性材料，以及抗氧化剂和生物活性化合物的载体。

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引用次数: 0

Enhancing the antioxidant and barrier properties of carboxymethyl chitosan/polyvinyl alcohol films by incorporating pomelo seed extract nano-emulsion 柚籽提取物纳米乳液增强羧甲基壳聚糖/聚乙烯醇膜的抗氧化和屏障性能

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-09-13 DOI: 10.1016/j.fbp.2025.09.009

Zhiwu Huang , Xinrui Tang , Qiang Peng , Qianhua Zhang , Huaicheng Qin , Yongguang Bi , Juan Guo , Fansheng Kong , Haijie Jin , Canzhao Chen , Huiyan Xu

In this research, the oil and alcohol phases were extracted from pomelo seeds and used to prepare nano-emulsions with whey protein (WP). Four bioactive films were developed by incorporating varying concentrations of pomelo seed extract nano-emulsions (PSE-NE) into carboxymethyl chitosan (CMCS)/polyvinyl alcohol (PVA) matrices. The preparation process of the nano-emulsion was investigated, and its microstructure was characterized via transmission electron microscopy (TEM). The interactions between the nano-emulsion and the film matrix were investigated through characterization via scanning electron microscopy (SEM), fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). Moreover, the physical properties and bioactivity of the films, such as mechanical properties, antioxidant activity, water vapor transmission rate (WVTR), UV-Vis light-blocking performance, and biodegradability were studied. The results identified CPP-3 film as the optimal film formulation, exhibiting strong antioxidant capacity with DPPH and ABTS radical scavenging rates of 87.66 ± 0.40 % and 92.25 ± 0.95 %, respectively. It demonstrated a water vapor transmission rate of 6.74 ± 1.04 g/m²/h, an opacity coefficient of 2.96 mm⁻¹, and a biodegradation rate of 56.51 ± 0.48 % after 25 days of soil burial. These findings highlight the promise of biodegradable films based on PSE-NE for environmentally friendly food packaging applications, providing a new way for valorizing plant-derived waste such as pomelo seeds in eco-friendly packaging solutions.

本研究从柚籽中提取油相和醇相，制备乳清蛋白纳米乳液。将不同浓度的柚籽提取物纳米乳液（PSE-NE）加入羧甲基壳聚糖(CMCS)/聚乙烯醇（PVA）基质中，制备了四种生物活性膜。研究了纳米乳液的制备工艺，并用透射电镜（TEM）对其微观结构进行了表征。通过扫描电镜（SEM）、傅里叶变换红外光谱（FTIR）、x射线衍射（XRD）和热重分析（TGA）表征纳米乳液与膜基质之间的相互作用。此外，还研究了膜的物理性能和生物活性，如机械性能、抗氧化性能、水蒸气透过率（WVTR）、UV-Vis阻光性能和生物降解性。结果表明，CPP-3膜具有较强的抗氧化能力，对DPPH和ABTS自由基的清除率分别为87.66±0.40 %和92.25±0.95 %。土壤埋藏25 d后，其水蒸气透过率为6.74 ± 1.04 g/m2/h，不透明度系数为2.96 mm−1，生物降解率为56.51 ± 0.48 %。这些发现突出了基于PSE-NE的可生物降解薄膜在环保食品包装应用中的前景，为在环保包装解决方案中对柚子种子等植物来源的废物进行评估提供了一种新的方法。

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引用次数: 0

Proof-of-concept: Ultrafiltration as a pre-treatment for the anion exchange chromatography of RuBisCO from alfalfa 概念验证：超滤作为紫花苜蓿RuBisCO阴离子交换色谱的预处理

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-10-13 DOI: 10.1016/j.fbp.2025.10.011

Hartono Tanambell , Marianne Danielsen , Anders Hauer Møller , Trine Kastrup Dalsgaard

Alfalfa is gaining interest as an alternative protein source. The European Food Safety Authority (EFSA) approval of alfalfa protein concentrate as novel food states presence of metabolites as a barrier for higher amount of consumption. A promising method for protein isolation is anion exchange chromatography, which is scalable through expanded bed chromatography. In this study, ultrafiltration-buffer exchange (UBE) and ultrafiltration-diafiltration was applied as an upstream and downstream processing to anion exchange chromatography, respectively. The performance of these techniques was compared to that of lab scale dilution and centrifugal spin filters. The result showed that UBE shortened the chromatography duration by 50 % and resulted in a cleaner column. However, downstream desalination through ultrafiltration-diafiltration resulted in concentrate with lower protein content (60.41 ± 0.84 %), compared to that obtained from centrifugal spin filters (90.49 ± 1.34 %), suggesting the need to carry out extra diafiltration steps. Untargeted metabolomics revealed that high absorption at 214 nm in the anion exchange flowthrough fraction could be attributed to di- and tripeptides also present in the ultrafiltration permeates. Polyphenols present in ultrafiltration permeate were absent from both flowthrough and 225 mM fraction, suggesting that they might bind strongly to the column. Various carboxylic acids were present in the ultrafiltration permeate, chromatography flowthrough, and 225 mM NaCl fraction. Contaminants eluting at 225 mM NaCl with high absorbance at 214 nm were not successfully identified although their occurrence was lower in UBE samples. These results suggest that UBE is promising as pre-treatment for purification of RuBisCO from alfalfa using anion exchange chromatography.

苜蓿作为一种替代蛋白质来源正引起人们的兴趣。欧洲食品安全局（EFSA）批准苜蓿蛋白浓缩物作为新型食品，指出其代谢物的存在是大量食用的障碍。阴离子交换色谱法是一种很有前途的蛋白质分离方法，它可以通过扩展床色谱法进行扩展。在本研究中，超滤-缓冲交换（UBE）和超滤-滤滤分别作为阴离子交换色谱的上游和下游处理。将这些技术的性能与实验室规模稀释和离心自旋过滤器的性能进行了比较。结果表明，UBE使色谱时间缩短了50% %，色谱柱更干净。然而，与离心自旋过滤器获得的浓缩物（90.49 ± 1.34 %）相比，下游通过超滤-滤除得到的浓缩物蛋白质含量（60.41 ± 0.84 %）较低，这表明需要进行额外的滤除步骤。非靶向代谢组学显示，在214 nm处阴离子交换流过部分的高吸收可能归因于超滤渗透物中也存在的二肽和三肽。超滤渗透液中存在的多酚在流过和225 mM馏分中均不存在，表明它们可能与柱结合强烈。超滤渗透液、色谱流和225 mM NaCl馏分中存在不同的羧酸。在225 mM NaCl洗脱的214 nm高吸光度的污染物虽然在UBE样品中的发生率较低，但未能成功鉴定。这些结果表明，UBE有望作为阴离子交换色谱法纯化紫花苜蓿中RuBisCO的前处理。

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引用次数: 0

Extraction and industrial valorization of collagen, gelatin, and hydroxyapatite from freshwater fish scales: A review 从淡水鱼鳞中提取胶原蛋白、明胶和羟基磷灰石及其工业价值：综述

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-10-05 DOI: 10.1016/j.fbp.2025.10.004

Md. Amjad Patwary , Md. Yasin , Md. Shofiul Azam , Mohammad Afzal Hossain

The fish processing industry generates significant waste, including scales, bones, and skin, contributing to environmental challenges. Freshwater fish scales offer potential for extracting valuable biomaterials such as collagen, gelatin, and hydroxyapatite, which have diverse industrial applications. Recent research has focused on extracting type I collagen, gelatin, and hydroxyapatite from fish scales as suitable alternatives to animal-derived products, addressing health concerns and religious restrictions in many parts of the world. The review emphasizes established methods for extracting collagen, gelatin, and hydroxyapatite from freshwater fish scales, along with their functional properties and diverse industrial applications. This study addresses a gap in literature by exploring the economic potential of valorizing whole freshwater fish scales, while focusing on established techniques for extraction of key biomaterials such as collagen, gelatin, and hydroxyapatite and emphasizes their diverse applications in the biomedical, food, and packaging sectors. This review also addresses the safety regulations and future prospects for valorizing fish scales. Through comprehensive analysis and exploration, this research contributes to sustainable resource utilization in the fish processing industry.

鱼类加工业产生大量废物，包括鱼鳞、鱼骨和鱼皮，对环境构成挑战。淡水鱼鳞提供了提取有价值的生物材料的潜力，如胶原蛋白、明胶和羟基磷灰石，这些材料具有多种工业应用。最近的研究集中在从鱼鳞中提取I型胶原蛋白、明胶和羟基磷灰石，作为动物源性产品的合适替代品，以解决世界上许多地方的健康问题和宗教限制。综述了从淡水鱼鳞中提取胶原蛋白、明胶和羟基磷灰石的方法，以及它们的功能特性和多种工业应用。本研究通过探索整个淡水鱼鳞片的经济潜力来填补文献空白，同时重点关注胶原蛋白、明胶和羟基磷灰石等关键生物材料的提取技术，并强调其在生物医学、食品和包装领域的多种应用。本文还讨论了鱼鳞加工的安全法规和未来前景。通过综合分析和探索，本研究有助于鱼类加工业资源的可持续利用。

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引用次数: 0

Protein-based wall materials to microencapsulate fish oil as source of EPA and DHA 蛋白质为基础的壁材料微胶囊鱼油作为EPA和DHA的来源

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-01 Epub Date: 2025-11-17 DOI: 10.1016/j.fbp.2025.11.015

Francisco de-la-Haba, Abraham Pajuelo, Teresa Antequera, Javier Durán, Trinidad Pérez-Palacios

This study aimed to develop highly stable, efficient and bioaccessible microcapsules containing EPA and DHA by using fish oil and evaluating different protein-based wall materials of plant and animal origin, with or without enzymatic treatment. Pea protein, soy protein, calcium caseinate, and caseinate combined with transglutaminase were employed as wall materials. Emulsions were prepared and subsequently spray-dried to obtain microcapsule powders. Both plant- and animal-derived proteins significantly influenced most quality parameters of the microcapsules, whereas the effect of transglutaminase addition was less pronounced, mainly impacting microencapsulation efficiency. All wall materials provided stability to the fish oil microcapsules, although plant proteins induced noticeable color changes. Caseinate, particularly when combined with transglutaminase, achieved the highest encapsulation efficiency. Moreover, caseinate exhibited superior performance in terms of EPA and DHA bioaccessibility and flow properties, making it the most suitable wall material. Overall, the findings highlight calcium caseinate as the preferred choice for fish oil microencapsulation to produce effective delivery vehicles of EPA and DHA.

本研究以鱼油为原料，对植物和动物源性不同的蛋白基壁材进行酶处理或不进行酶处理，旨在开发出高度稳定、高效、生物可及的含有EPA和DHA的微胶囊。以豌豆蛋白、大豆蛋白、酪蛋白酸钙、酪蛋白酸钙联合谷氨酰胺转胺酶作为壁材。制备乳剂，然后喷雾干燥得到微胶囊粉末。植物源性蛋白和动物源性蛋白对微胶囊的大部分品质参数均有显著影响，而转谷氨酰胺酶的添加对微胶囊的影响不太明显，主要影响微胶囊的效率。尽管植物蛋白引起了明显的颜色变化，但所有的壁材都为鱼油微胶囊提供了稳定性。酪蛋白酸盐，特别是与谷氨酰胺转胺酶结合时，达到了最高的包封效率。酪蛋白酸盐在EPA和DHA的生物可及性和流动性能方面表现优异，是最合适的壁材。总的来说，研究结果强调酪蛋白酸钙是鱼油微胶囊的首选，可以产生有效的EPA和DHA递送载体。

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引用次数: 0