Food and Bioproducts Processing最新文献_第4页

Isoflavone-rich extracts from okara using supercritical fluid extraction: Kinetic modeling and characterization 利用超临界流体萃取技术从秋葵中提取富含异黄酮的提取物：动力学建模与表征

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-28 DOI: 10.1016/j.fbp.2024.09.019

Elixabet Díaz-de-Cerio , Pedro Barea , Rodrigo Melgosa , Alba Ester Illera , María Teresa Sanz , Sagrario Beltrán

The lipid fraction and bioactive compounds from okara, a by-product of soybean processing, have been extracted by supercritical fluid extraction. The effect of operating parameters, pressure (20–40 MPa), temperature (40–80 ºC), and cosolvent usage (0–10 %wt. ethanol), on the extraction kinetics has been investigated, satisfactorily correlating the experimental data to the Sovová’s model. Extraction yield by SFE ranged from 0.1014 g extract/g insoluble solid (IS) at 20 MPa and 80 ºC to 0.1081 g extract/g IS at 30 MPa and 60 ºC (from 9.2 to 9.8 g extract/100 g okara, respectively), similar to Soxhlet extraction with n-hexane (9.2 ± 0.2 g extract/100 g). Pressure positively affected the initial extraction rate, which also increased with temperature except for the lowest pressure of 20 MPa, exhibiting a typical cross-over behavior. The use of ethanol as cosolvent promoted the extraction of phenolic compounds and isoflavones, and was less important in the extraction of tocopherols. Extraction yield using ethanol increased up to 0.1100 g extract/g IS at 40 MPa and 40 ºC. As expected, the higher content of bioactive compounds in the sc-CO₂ + ethanol extracts positively affected the antioxidant capacity, with the maximum found at 20 MPa, 40 ºC and 10 %wt. ethanol.

采用超临界流体萃取法萃取了大豆加工副产品黄卡拉中的脂质部分和生物活性化合物。研究了压力（20-40 兆帕）、温度（40-80 ºC）和共溶剂用量（0-10 % 重量百分比乙醇）等操作参数对萃取动力学的影响，实验数据与索沃娃模型的相关性令人满意。在20兆帕和80摄氏度时，SFE的萃取率为0.1014克萃取物/克不溶性固体（IS）；在30兆帕和60摄氏度时，萃取率为0.1081克萃取物/克不溶性固体（每100克黄原胶的萃取率分别为9.2克到9.8克），与使用正己烷的索氏萃取（9.2 ± 0.2克萃取物/100克）相似。压力对初始萃取率有积极影响，除了最低压力为 20 兆帕外，萃取率也随温度升高而增加，表现出典型的交叉行为。使用乙醇作为助溶剂可促进酚类化合物和异黄酮的萃取，但对生育酚的萃取影响较小。在 40 兆帕和 40 ºC 条件下，乙醇的萃取率最高可达 0.1100 克萃取物/克 IS。不出所料，sc-CO2 + 乙醇提取物中生物活性化合物含量较高，对抗氧化能力产生了积极影响，在 20 兆帕、40 ºC 和 10 %wt.

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引用次数: 0

Technical-economic assessment of 5-hydroxymethylfurfural production via catalytic hydrothermal synthesis from lignocellulosic forest residues 利用木质纤维素森林残留物进行催化水热合成生产 5-羟甲基糠醛的技术经济评估

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-27 DOI: 10.1016/j.fbp.2024.09.021

Tiago Guimarães, Ricardo de Carvalho Bittencourt, Ana Márcia Macedo Ladeira Carvalho, Sebastião Renato Valverde, Marcelo Moreira da Costa

The 5-hydroxymethylfurfural is a high-value chemical that can be used as a raw material for the synthesis of biofuels and bioplastics. The use of forest woody residues as a source of biomass reduces the costs and environmental impacts associated with the process. The objective of this study was to optimize the conditions of catalytic hydrothermal synthesis of 5-HMF from the residue of three biomasses, and to carry out a technical-economic assessment of the feasibility of the process on an industrial scale. The materials used were forest woody residues, distilled water, acetic acid (CH₃COOH), and niobium oxide (Nb2O5) as a reaction catalyst.The identification and quantification of the 5-HMF yield was monitored by high-performance liquid chromatography (HPLC). For the technical-economic assessment, the CAPCOST Estimation Software® was used, which allows estimating the capital and operation costs, as well as the financial indicators of the project. Under optimized conditions, the maximum yield of 5-HMF was 32.6 %, based on dry mass (bdm). The technical-economic assessment (TEA) indicated that the project is economically viable, considering a selling price of 5-HMF of 2.16 dollars/kg. The main financial indicators were CAPEX of 197.83 million dollars, OPEX of 99.78 million dollars, net present value (NPV) of 197.83 million dollars, internal rate of return (IRR) of 44 %, and the payback time of 2.25 years.

5- 羟甲基糠醛是一种高价值化学品，可用作合成生物燃料和生物塑料的原料。使用森林木质残渣作为生物质来源可降低该工艺的成本和对环境的影响。本研究旨在优化从三种生物质残渣中催化水热合成 5-HMF 的条件，并对该工艺在工业规模上的可行性进行技术经济评估。使用的材料为森林木质残留物、蒸馏水、乙酸（CH3COOH）和作为反应催化剂的氧化铌（Nb2O5）。在进行技术经济评估时，使用了 CAPCOST Estimation Software®，该软件可以估算资本和运营成本，以及项目的财务指标。在优化条件下，按干重（bdm）计算，5-HMF 的最高产量为 32.6%。技术经济评估（TEA）表明，考虑到 5-HMF 的销售价格为 2.16 美元/公斤，该项目在经济上是可行的。主要财务指标为：资本支出（CAPEX）1.9783 亿美元，运营支出（OPEX）9978 万美元，净现值（NPV）1.9783 亿美元，内部收益率（IRR）44%，投资回收期 2.25 年。

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引用次数: 0

Effect of production scale on the techno-economic viability and environmental life cycle analysis of lactic acid production in a sugarcane biorefinery 生产规模对甘蔗生物精炼厂乳酸生产的技术经济可行性和环境生命周期分析的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-27 DOI: 10.1016/j.fbp.2024.09.020

Mensah S. Brobbey, Janus P. Louw, Jeanne Louw, Johann F. Görgens

Biorefineries are vital for advancing circular economy and reducing the effects of products fossil fuels derived products on the environment. However, biorefineries often operate at smaller production scales than fossil refineries due to limited feedstock availability, which may be addressed by centralised processing with multiple feedstock sources. Lactic acid (LA) has several industrial applications and is a platform chemical used to produce products like acrylic acid and propylene glycol. The economic and environmental performances of an integrated biorefinery at different production scales, through different levels of feedstock centralization, were investigated, to determine the optimal scale for sugarcane-based LA production. Decreasing values for the minimum selling price (MSP; 1312–849 US$.t⁻¹) and increasing internal rates of return (IRRs; 31–64 %) were observed with increasing conversion scales of sugarcane A-molasses. The MSPs decrease from 90 to 450 kt_LA.y⁻¹ with small improvements in profitability beyond 450 kt_LA.y⁻¹, as confirmed by stochastic financial uncertainty analysis. In the environmental assessment, a linear increase was observed across all impact categories, mainly due to the added fuel consumption for feedstock transportation. LA production had a GWP100 range of 0.87–0.95 kg CO₂-eq.kg_LA⁻¹ and an abiotic depletion potential of 12–13 MJ.kg_LA⁻¹ which increased as the scale increased. In the ozone depletion category emissions of 9.96×10⁻⁸-1.13×10⁻⁷ kg CFC-11 eq.kg_LA⁻¹ comparable to other studies available in literature. Similarly, emission ranges of 1.11–1.17, 0.63–0.66, and 1581–1641 kg 1,4-DB eq.kg_LA⁻¹ were obtained in the human toxicity, freshwater and marine aquatic ecotoxicity categories as the scale increased. Environmentally the smallest scale at which transportation of feedstock was avoided (i.e. 90 kt_LA.y⁻¹) is preferred as opposed to 450 kt_LA.y⁻¹ for economic performance.

生物精炼厂对于推进循环经济和减少化石燃料衍生产品对环境的影响至关重要。然而，由于原料供应有限，生物精炼厂的生产规模往往小于化石精炼厂，这可以通过多种原料来源的集中加工来解决。乳酸（LA）有多种工业用途，是一种用于生产丙烯酸和丙二醇等产品的平台化学品。通过不同程度的原料集中化，研究了不同生产规模的综合生物精炼厂的经济和环境性能，以确定以甘蔗为原料的 LA 生产的最佳规模。随着甘蔗 A-蔗糖转化规模的扩大，最低销售价格（MSP；1312-849 美元/吨-1）不断降低，内部收益率（IRR；31-64%）不断提高。随机财务不确定性分析证实，从 90 ktLA.y-1 到 450 ktLA.y-1，最大生产潜力下降，超过 450 ktLA.y-1 后盈利能力略有提高。在环境评估中，所有影响类别都呈线性增长，这主要是由于原料运输增加了燃料消耗。LA 生产的 GWP100 范围为 0.87-0.95 kg CO2-eq.kgLA-1，非生物消耗潜能值为 12-13 MJ.kgLA-1，随着规模的扩大而增加。臭氧消耗类的排放量为 9.96×10-8-1.13×10-7 kg CFC-11eq.kgLA-1，与文献中的其他研究结果相当。同样，随着规模的增加，在人类毒性、淡水和海洋水生生态毒性类别中，排放范围分别为 1.11-1.17、0.63-0.66 和 1581-1641 kg 1,4-DB eq.kgLA-1。从环境角度看，避免原料运输的最小规模（即 90 ktLA.y-1）比 450 ktLA.y-1 更符合经济效益。

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引用次数: 0

Impact of alkaline solution, carbohydrase and biomass:solvent on extraction efficiency, and protein and amino acid profiles of Palmaria palmata protein extracts 碱性溶液、碳水化合物酶和生物质：溶剂对棕榈蛋白提取物的提取效率以及蛋白质和氨基酸谱的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-26 DOI: 10.1016/j.fbp.2024.09.017

Anthony Temitope Idowu , Miryam Amigo-Benavent , Richard.J. FitzGerald

The impact of using different alkaline solutions, carbohydrases and changing the biomass to solvent volume ratio (biomass:solvent) on protein content, protein and biomass yield recovery in protein enriched extracts from Palmaria palmata (red seaweed) was studied. Protein extraction with alkaline solutions, i.e., NaOH, KOH, Ca(OH)₂ and Na₂CO₃ (at 0.12 M) along with (and without) carbohydrases B, BD and L (containing xylanase, a mixture of xylanase and cellulase, and a combination of cellulase, xylanase and polygalacturonase activities, respectively) was assessed. Principal component analysis was used to assess correlations (if any) between the different alkaline solvents, carbohydrases and changing biomass:solvent on extraction efficiency. The highest protein content (56.64 ± 0.43 % (w/w)) was obtained using KOH assisted extraction with carbohydrase BD. The highest protein and biomass yield recoveries (70.65 ± 1.38 and 21.99 ± 0.43 % (w/w), respectively) were obtained on extraction using NaOH assisted with carbohydrases BD. Electrophoretic, amino acid profile and amino acid score analyses displayed differences depending on the alkaline solvent used for protein extraction. Appropriate selection of the alkaline solvent, carbohydrase and biomass:volume combination during protein extraction can contribute to the production of compositionally enhanced high-quality protein concentrates from P. palmata.

研究了使用不同的碱性溶液、碳水化合物酶以及改变生物质与溶剂的体积比（生物质：溶剂）对棕榈叶（红藻）蛋白质富集提取物中蛋白质含量、蛋白质和生物质产量回收率的影响。评估了使用碱性溶液，即 NaOH、KOH、Ca(OH)2 和 Na2CO3（0.12 M）以及（或不使用）碳水化合物酶 B、BD 和 L（分别含有木聚糖酶、木聚糖酶和纤维素酶的混合物以及纤维素酶、木聚糖酶和聚半乳糖醛酸酶活性的组合）提取蛋白质的情况。采用主成分分析法评估了不同碱性溶剂、碳水化合物酶和生物质：溶剂变化对提取效率的相关性（如有）。使用 KOH 辅助萃取和碳水化合物酶 BD，蛋白质含量最高（56.64 ± 0.43 %（w/w））。在使用 NaOH 和碳水化合物酶 BD 辅助提取时，蛋白质和生物量的回收率最高（分别为 70.65 ± 1.38 % 和 21.99 ± 0.43 %（重量比））。电泳分析、氨基酸谱分析和氨基酸评分分析因提取蛋白质所用的碱性溶剂不同而存在差异。在蛋白质提取过程中适当选择碱性溶剂、碳水化合物酶和生物量：体积组合，有助于从棕榈属植物中生产出成分更优的优质浓缩蛋白质。

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引用次数: 0

In-pack radio frequency heating of liquid whole egg: Effect of agitation on heating rate and uniformity 包装内射频加热液态全蛋：搅拌对加热速度和均匀性的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-26 DOI: 10.1016/j.fbp.2024.09.018

Mahir Cin, T. Koray Palazoğlu

In-pack radio frequency (RF) heating of liquid whole egg (LWE) was performed using a 27.12 MHz parallel-plate radio frequency (RF) system. To do this, 600 mL of LWE was subjected to RF heating in a RF-transparent plastic bag (140 mm×180 mm). Experiments were conducted at two different electrode gap settings (98 and 103 mm) with and without orbital movement in the horizontal plane. The effect of agitation on heating rate and uniformity was investigated for varying rpm’s (120 and 140 rpm) for a constant orbit diameter (35 mm). An orbital shaker was specially designed and coupled with the RF system for this purpose. Fiber optic probes were used to continuously measure the temperature of LWE at four different internal locations. Temperature uniformity index (TUI) was calculated to evaluate the uniformity of heating. Coagulation of LWE around the headspace was inevitable when the package was stationary during the treatment. Agitation brought about by orbital movement prevented the coagulation. Drastic improvement in heating rate and uniformity was observed upon orbital movement of LWE at all conditions.

使用 27.12 MHz 的平行板射频（RF）系统对液态全蛋（LWE）进行了包装内射频（RF）加热。为此，在一个射频透明塑料袋（140 毫米×180 毫米）中对 600 毫升液态全蛋液进行射频加热。实验在两种不同的电极间隙设置（98 毫米和 103 毫米）下进行，在水平面内有轨道运动和无轨道运动。在轨道直径（35 毫米）不变的情况下，研究了不同转速（120 和 140 转/分钟）下搅拌对加热速率和均匀性的影响。为此专门设计了一个轨道振动器，并与射频系统相连。光纤探头用于连续测量 LWE 四个不同内部位置的温度。通过计算温度均匀性指数（TUI）来评估加热的均匀性。在处理过程中，当包装静止不动时，LWE 不可避免地会在顶空周围凝结。轨道运动带来的搅拌防止了凝结。在所有条件下，LWE 的轨道运动都能显著提高加热速度和均匀性。

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引用次数: 0

Characterization and in vitro bioaccessability of optimized chia oil-Capsul-sodium alginate microparticles obtained by 3 nozzle spray-drying 通过 3 个喷嘴喷雾干燥获得的优化奇异果油-胶囊-海藻酸钠微颗粒的特性和体外生物可接受性

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-26 DOI: 10.1016/j.fbp.2024.09.012

Rudy Alvarez , Paz Robert , Alejandra Quintriqueo , Felipe Oyarzún-Ampuero , Alan Mackie , Amelia Torcello-Gómez

The influence sodium alginate (SA) as an outer layer agent on bioaccessibility and matrix food release of purified chia oil (PCO) microencapsulated was investigated. PCO microparticles with Capsul were elaborated and optimized by response surface methodology (RSM), and then as an encapsulating agent and SA as an outer layer (PCO-Capsul/SA) were designed by mini spray-drying with 2 fluid nozzle (2-N) and 3 fluid nozzle (3-N). The optimal conditions obtained for PCO-Capsul system were: dryer inlet temperature of 114 °C and PCO:Capsul relation of 1:5.42. After the addition of SA out layer, PCO-Capsul/SA microparticles were subjected to in vitro static gastrointestinal digestion. PCO-Capsul/SA occurred mainly in the intestinal phase, showing the suitability of SA as an intestine-site release polymer. However, when PCO-Capsul/SA was incorporated into a yoghurt (Y), microparticles showed a significantly lower PCO matrix food release and bioaccessibility after in vitro digestion than PCO-Capsul-Y microparticles, due to their interaction between SA and Y. SA spray-dried by 3-N showed great potential for vehiculation of omega-3 rich oils in the future incorporation and develop of functional foods.

Tweetable abstract

This research shows the role of the sodium alginate incorporation by 3 nozzle spray dryer when the design of an intestine-delivery food ingredient is intended.

研究了海藻酸钠（SA）作为外层剂对纯化奇异果油（PCO）微胶囊的生物可及性和基质食物释放的影响。采用响应面法（RSM）对含有 Capsul 的 PCO 微胶囊进行了阐述和优化，然后通过 2 流体喷嘴（2-N）和 3 流体喷嘴（3-N）的微型喷雾干燥法设计了作为封装剂和作为外层剂的 SA（PCO-Capsul/SA）。PCO-Capsul 系统的最佳条件是：干燥器入口温度为 114 °C，PCO:Capsul 的比例为 1:5.42。在加入 SA 外层后，PCO-Capsul/SA 微粒被置于体外静态胃肠消化。PCO-Capsul/SA 主要出现在肠道阶段，这表明 SA 适合作为肠道释放聚合物。然而，当 PCO-Capsul/SA 加入酸奶（Y）中时，由于 SA 和 Y 之间的相互作用，微颗粒在体外消化后显示出的 PCO 基质食物释放和生物可及性明显低于 PCO-Capsul-Y 微颗粒。经 3-N 喷雾干燥的海藻酸钠在富含欧米加-3 的油脂的载体化方面具有巨大潜力，可用于未来功能食品的添加和开发。

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引用次数: 0

CcCHIL, a type IV chalcone isomerase that can improve (2S)-naringenin production in Saccharomyces cerevisiae CcCHIL 是一种 IV 型查尔酮异构酶，可提高酿酒酵母中 (2S) 柚皮苷的产量

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-22 DOI: 10.1016/j.fbp.2024.09.016

Sisheng Zhang , Jianmu Su , Tsan-Yu Chiu , Jintao Fang , Xiangxiu Liang , Zhuoyuan He , Shancen Zhao , Hong Wu

(2S)-Naringenin, a crucial precursor in plant flavonoid synthesis, holds significance in diverse biological processes and potential therapeutic applications for human diseases. The economically valuable Citrus reticulata 'Chachi' citrus cultivar, renowned for its flavonoid-rich peel, was subjected to integrated transcriptomic and metabolomic analysis in this study to reveal patterns of flavonoid accumulation. The analysis revealed a strong correlation between the expression pattern of the gene encoding type IV chalcone isomerase (chalcone isomerase like, CHIL), CcCHIL, and the accumulation of flavonoids in C. reticulata 'Chachi' peel. Previous studies have demonstrated that type IV chalcone isomerase can enhance flavonoid accumulation in citrus and improve the catalytic efficiency of chalcone synthase in vitro, thereby increasing the titer of (2S)-naringenin. In this study, we constructed a genetically engineered yeast strain capable of de novo synthesis of (2S)-naringenin. We found that CcCHIL can increase (2S)-naringenin production in engineered yeast by 13.60 %. Subsequently, we conducted experiments using CHILs from various species and found that GmCHIL from Glycine max can increase (2S)-naringenin production in engineered yeast by 45.35 %. Sequence alignment, molecular docking predictions and site-directed mutagenesis showed that Asn151 is one of the key sites for CHIL improving (2S)-naringenin production. Our study unveiled CcCHIL's crucial role in flavonoid biosynthesis in C. reticulata 'Chachi' peel and paving the way for future synthesis of intricate and economically valuable flavonoids.

(2S)-柚皮苷是植物类黄酮合成过程中的一种重要前体物，在多种生物过程中具有重要意义，对人类疾病具有潜在的治疗作用。本研究对以果皮富含类黄酮而闻名的具有经济价值的柑橘栽培品种Citrus reticulata 'Chachi'进行了转录组和代谢组的综合分析，以揭示类黄酮的积累模式。分析结果表明，编码 IV 型查尔酮异构酶（类似查尔酮异构酶，CHIL）的基因 CcCHIL 的表达模式与 C. reticulata 'Chachi' 果皮中黄酮类化合物的积累之间存在很强的相关性。以前的研究表明，IV型查尔酮异构酶能增强柑橘中黄酮类化合物的积累，提高查尔酮合成酶在体外的催化效率，从而提高（2S）-柚皮苷的滴度。在这项研究中，我们构建了一种能够从头合成（2S）-柚皮苷的基因工程酵母菌株。我们发现，CcCHIL 可使工程酵母菌的(2S)-柚皮苷产量增加 13.60%。随后，我们利用不同物种的 CHIL 进行了实验，发现来自 Glycine max 的 GmCHIL 能使工程酵母的 (2S)- 柚皮苷产量提高 45.35%。序列比对、分子对接预测和定点突变表明，Asn151是CHIL提高(2S)-柚皮苷产量的关键位点之一。我们的研究揭示了 CcCHIL 在 C. reticulata 'Chachi' 果皮类黄酮生物合成中的关键作用，为将来合成复杂且具有经济价值的类黄酮铺平了道路。

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引用次数: 0

Fabrication and characterization of novel biodegradable films based on tomato seed mucilage and gelatin plasticized with polyol mixtures 基于番茄籽粘液和明胶与多元醇混合物塑化的新型生物可降解薄膜的制作和特性分析

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-21 DOI: 10.1016/j.fbp.2024.09.015

Samira Davoudi, Mohsen Zandi, Ali Ganjloo

This research evaluates the potential of tomato seed mucilage (TSM) –gelatin (Ge) in producing edible films. Edible films were made using various ratios of TSM to Ge (1:0, 0.33:0.66, 0.66:0.33, and 0:1). Moreover, the effects of including three types of plasticizers, namely, glycerol (Gly), sorbitol (Sor), and polyethylene glycol (PEG) were investigated. The findings indicated that adding Ge did not affect the thickness of the films (p>0.05). However, it significantly increased the tensile strength (TS) and Young's modulus while reducing the elongation at break (EB) (p˂0.05). A higher TSM ratio significantly increased total color difference (ΔE) and yellowness index while decreasing film lightness and whiteness index (WI) (p˂0.05). The contact angle significantly decreased with the TSM ratio increases and Ge decreases (p˂0.05). Moreover, this led to higher opacity, solubility, moisture content, oxygen permeability, water vapor permeability (WVP), swelling, and moisture absorption (p˂0.05). Adding a plasticizer had no effect on the films' opacity, contact angle, and thickness. The Gly-containing film featured the highest EB, solubility, moisture content, swelling, WVP, oxygen permeability, and moisture absorption. The Sor-containing film had the highest TS. TSM concentration was directly correlated with increased film heterogeneity and surface roughness. X-ray diffraction peaks became sharper by increasing TSM concentration and including PEG, while Gly inclusion produced ordered broad peaks.

本研究评估了番茄种子粘液（TSM）-明胶（Ge）在生产可食用薄膜方面的潜力。使用不同比例的 TSM 和 Ge（1:0、0.33:0.66、0.66:0.33 和 0:1）制作了可食用薄膜。此外，还研究了加入甘油（Gly）、山梨醇（Sor）和聚乙二醇（PEG）这三种增塑剂的效果。结果表明，添加 Ge 不会影响薄膜的厚度（p>0.05）。但是，它能明显提高拉伸强度（TS）和杨氏模量，同时降低断裂伸长率（EB）（p˂0.05）。较高的 TSM 比率会明显增加总色差 (ΔE) 和黄度指数，同时降低薄膜亮度和白度指数 (WI)（p˂0.05）。接触角随着 TSM 比率的增加和 Ge 的减少而明显减小（p˂0.05）。此外，这还会导致不透明度、溶解性、含水量、氧气渗透性、水蒸气渗透性（WVP）、膨胀性和吸湿性增加（p˂0.05）。添加增塑剂对薄膜的不透明度、接触角和厚度没有影响。含 Gly 的薄膜具有最高的 EB、溶解性、含水量、膨胀性、WVP、透氧性和吸湿性。含 Sor 的薄膜具有最高的 TS。TSM 浓度与薄膜异质性和表面粗糙度的增加直接相关。随着 TSM 浓度的增加和 PEG 的加入，X 射线衍射峰变得更尖锐，而 Gly 的加入则产生了有序的宽峰。

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引用次数: 0

Investigation of near infrared and Raman fibre optic process sensors for protein determination in milk protein concentrate 用于测定浓缩牛奶蛋白中蛋白质的近红外和拉曼光纤过程传感器的研究

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-19 DOI: 10.1016/j.fbp.2024.09.013

Yuanyuan Pu , Ming Zhao , Colm O’Donnell , Norah O’Shea

This study investigated the potential of two fibre optic process sensors based on near infrared (NIR) or Raman spectroscopic technology for protein measurement in milk protein concentrate (MPC). Partial least squares (PLS) models were developed using NIR, Raman, and fusion of NIR and Raman spectra data. Calibration models developed were optimized by selecting different spectral pre-treatment methods and spectral regions. Overall, the three optimal models (NIR, Raman, fused NIR and Raman) yielded R²p values >0.9 and RMSEP values in the range of 0.168–0.185 %. The optimised fusion model outperformed all Raman models and had a similar protein prediction accuracy (R²p = 0.911 and RMSEP = 0.178 %) compared to the optimised NIR model (R²p = 0.917 and RMSEP =0.168 %). Results of the study demonstrated that both NIR and Raman process probes can be used as process analytical technology (PAT) tools for inline protein measurements of MPC post membrane filtration.

本研究调查了基于近红外（NIR）或拉曼光谱技术的两种光纤过程传感器在牛奶浓缩蛋白（MPC）蛋白质测量中的潜力。利用近红外光谱、拉曼光谱以及近红外光谱和拉曼光谱数据的融合建立了偏最小二乘法（PLS）模型。通过选择不同的光谱预处理方法和光谱区域，对所开发的校准模型进行了优化。总体而言，三个最佳模型（近红外光谱、拉曼光谱、融合近红外光谱和拉曼光谱）的 R2p 值为 0.9，RMSEP 值在 0.168-0.185 % 之间。与优化的近红外模型（R2p = 0.917，RMSEP = 0.168 %）相比，优化的融合模型优于所有拉曼模型，蛋白质预测准确率（R2p = 0.911，RMSEP = 0.178 %）与之相近。研究结果表明，近红外和拉曼过程探针可作为过程分析技术 (PAT) 工具，用于膜过滤后 MPC 的在线蛋白质测量。

引用次数: 0

Shell structure impacts Camellia oleifera fruit splitting during dehydration 脱水过程中果壳结构对油茶果实分裂的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-19 DOI: 10.1016/j.fbp.2024.09.006

Pei Yang, Hanyu Fu, Penggeng Sun, Liuyang Ren, Zhaohui Zheng, Jingshen Xu, Lanlan Lv, Qing Liang, Deyong Yang

This study aims to enhance the processing efficiency of Camellia oleifera fruit by exploring the potential relationship between the splitting characteristics during the dehydration process and the structure of the C. oleifera shell. The analysis focused on understanding the structural changes, moisture transfer, and shrinkage strain of the C. oleifera shell during the shelling processes. The results indicate that the essence of shelling C. oleifera fruit is the uneven shrinkage strain of the shell after dehydration, with the uneven shrinkage of the mesocarp being the primary driving force for the separation of seeds from the shell. Because the cuticle prevents moisture transfer and epidermal cells limit shell shrinkage, the exocarp can restrict the efficiency of the shell shrinkage strain. By damaging the exocarp structure, the shelling efficiency of C. oleifera fruit can be increased by more than 50 % under both natural drying and hot air drying. Moreover, under hot air drying, the shelling efficiency of C. oleifera fruit with a damaged exocarp at a low temperature (55 ℃) is 12.5 % higher than that of undamaged fruit at a high temperature (75 ℃). This study provides new insights into improving the shelling efficiency of C. oleifera fruit.

本研究旨在通过探索脱水过程中的劈裂特征与油茶果壳结构之间的潜在关系，提高油茶果的加工效率。分析的重点是了解脱壳过程中油茶果壳的结构变化、水分转移和收缩应变。结果表明，油橄榄果实脱壳的本质是脱水后果壳的不均匀收缩应变，中果皮的不均匀收缩是种子与果壳分离的主要动力。由于角质层阻止水分传递，表皮细胞限制果壳收缩，因此外果皮会限制果壳收缩应变的效率。通过破坏外果皮结构，油橄榄果实的脱壳效率在自然干燥和热风干燥条件下都能提高 50% 以上。此外，在热风干燥条件下，外果皮受损的油橄榄果实在低温（55 ℃）下的脱壳效率比未受损果实在高温（75 ℃）下的脱壳效率高 12.5%。这项研究为提高油橄榄果实的脱壳效率提供了新的见解。

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