Polyphenols, known for their antioxidant and anti-inflammatory properties, are widely used in functional foods, cosmetics, and pharmaceuticals. However, their chemical stability is often compromised due to auto-oxidation and enzymatic degradation. Acidic extraction environments have been shown to reduce degradation, yet their effect on extraction performance has not been systematically investigated. This study investigates the impact of pH and temperature on the extraction efficiency of polyphenols from Salvia officinalis, comparing batch and continuous (fixed-bed column) systems using distilled water, acidified water (pH 2.5), and a 50 % v/v ethanol–water mixture. Batch tests demonstrated that acidification of water did not significantly affect extraction yields compared to distilled water: at 25 °C, the maximum extractable polyphenols () were 125.66 ± 2.82 mg/g and 124.09 ± 1.17 mg/g for distilled and acidified water, respectively; at 70 °C, values were 136.33 ± 3.01 mg/g and 131.83 ± 1.90 mg/g. The hydroalcoholic benchmark achieved higher yields (147.70 ± 3.26 mg/g at 25 °C and 155.52 ± 3.58 mg/g at 70 °C), about 20 % greater than purely aqueous solvents. Column tests confirmed these findings, with estimated values in good agreement with batch data and Freundlich isotherm models accurately fitting the continuous system behavior. Confidence intervals for parameter estimates were consistently below 15 %, indicating high reliability. Overall, the results demonstrate that while acidic conditions may help preserve polyphenol stability, they do not significantly affect extraction efficiency. These findings provide novel experimental data and a robust modeling approach for designing stability-oriented, scalable, and environmentally sustainable polyphenol extraction processes.
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