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Stability-oriented extraction of polyphenols from Salvia officinalis: Assessing the effect of pH in batch and continuous systems 以稳定性为导向提取鼠尾草中多酚:评价pH值在间歇和连续体系中的影响
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-11-07 DOI: 10.1016/j.fbp.2025.11.006
Lorenzo De Paola , Daniela De Cata , Alice Bertino , Chiara Guarnerio , Luigi Nataloni , Vincenzo Piemonte , Leone Mazzeo
Polyphenols, known for their antioxidant and anti-inflammatory properties, are widely used in functional foods, cosmetics, and pharmaceuticals. However, their chemical stability is often compromised due to auto-oxidation and enzymatic degradation. Acidic extraction environments have been shown to reduce degradation, yet their effect on extraction performance has not been systematically investigated. This study investigates the impact of pH and temperature on the extraction efficiency of polyphenols from Salvia officinalis, comparing batch and continuous (fixed-bed column) systems using distilled water, acidified water (pH 2.5), and a 50 % v/v ethanol–water mixture. Batch tests demonstrated that acidification of water did not significantly affect extraction yields compared to distilled water: at 25 °C, the maximum extractable polyphenols (qtot) were 125.66 ± 2.82 mg/g and 124.09 ± 1.17 mg/g for distilled and acidified water, respectively; at 70 °C, values were 136.33 ± 3.01 mg/g and 131.83 ± 1.90 mg/g. The hydroalcoholic benchmark achieved higher yields (147.70 ± 3.26 mg/g at 25 °C and 155.52 ± 3.58 mg/g at 70 °C), about 20 % greater than purely aqueous solvents. Column tests confirmed these findings, with estimated qtot values in good agreement with batch data and Freundlich isotherm models accurately fitting the continuous system behavior. Confidence intervals for parameter estimates were consistently below 15 %, indicating high reliability. Overall, the results demonstrate that while acidic conditions may help preserve polyphenol stability, they do not significantly affect extraction efficiency. These findings provide novel experimental data and a robust modeling approach for designing stability-oriented, scalable, and environmentally sustainable polyphenol extraction processes.
多酚以其抗氧化和抗炎特性而闻名,被广泛用于功能性食品、化妆品和药品中。然而,由于自氧化和酶降解,它们的化学稳定性经常受到损害。酸性萃取环境已被证明可以减少降解,但其对萃取性能的影响尚未得到系统的研究。本研究考察了pH和温度对丹参中多酚提取效率的影响,比较了蒸馏水、酸化水(pH 2.5)和50 % v/v乙醇-水混合物的间歇和连续(固定床柱)系统。批量试验表明,与蒸馏水相比,酸化水对提取率没有显著影响:在25°C下,蒸馏水和酸化水的最大可提取多酚(qtot)分别为125.66±2.82 mg/g和124.09±1.17 mg/g;70℃时分别为136.33±3.01 mg/g和131.83±1.90 mg/g。在25°C和70°C条件下,氢乙醇的收率分别为147.70±3.26 mg/g和155.52±3.58 mg/g,比纯水溶剂的收率高20%左右。柱测试证实了这些发现,估计的qtot值与批数据和Freundlich等温线模型非常吻合,准确地拟合了连续系统的行为。参数估计的置信区间始终低于15%,表明高可靠性。总的来说,结果表明,虽然酸性条件可能有助于保持多酚的稳定性,但它们对提取效率没有显著影响。这些发现为设计面向稳定性、可扩展和环境可持续的多酚提取工艺提供了新颖的实验数据和强大的建模方法。
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引用次数: 0
Deep eutectic solvents as green media for the extraction of hop cone antioxidants for food applications: Process optimization through interpolation method 深共晶溶剂作为绿色介质用于食品中啤酒花果抗氧化剂的提取:通过插值法优化工艺
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-11-07 DOI: 10.1016/j.fbp.2025.11.004
Victoria Vorobyova , Margarita Skiba , Maksym Dudka , Oleksii Kosohin , Georgii Vasyliev
A deep eutectic solvent (DES) composed of L-proline and glycerol was synthesized and characterized to evaluate its potential as a green extractant. Key physicochemical properties, including pH, surface tension, polarity, and conductivity, were measured. Quantum chemical calculations predicted favorable interactions between the components, supported by FTIR and proton nuclear magnetic resonance (¹H NMR) spectroscopy, which confirmed the formation of new hydrogen bonds. The DES was used to extract natural compounds from hop cones (Humulus lupulus L.) and compared with conventional solvents such as isopropanol and water. For the first time, the extraction efficiency of the L-proline–glycerol DES was evaluated across five hop varieties: "Clone 18–11t", "Glow", "Bitter", "Aromatic", and "October". An interpolation-based optimization method was used to reduce experimental workload and enhance resource efficiency. The algorithm was implemented in Java using IntelliJ IDEA Ultimate, with Apache Commons and Apache POI for mathematical computations, and JavaFX for graphical visualization, providing a robust and flexible framework for smooth data reconstruction. The effect of solvent on the content of phenolic compounds and flavonoids, as well as the antioxidant activity of hop extracts, was studied. Antioxidant activity was assessed via electrochemical and spectrophotometric techniques, revealing that both hop variety and solvent system significantly influenced antioxidant capacity. Gas chromatography–mass spectrometry showed distinct profiles of bitter acids and aromatic compounds across varieties. Notably, DES extracts provided superior preservation of antioxidant phenolic compounds compared to ethanol. The environmental sustainability of the extraction methods was assessed using the Analytical GREEnness Metric Approach (AGREE), confirming the DES system as a greener alternative. These findings demonstrate the viability of DES as an eco-friendly and effective solvent for extracting bioactive compounds from plant materials.
合成了一种由l -脯氨酸和甘油组成的深度共熔溶剂(DES),并对其进行了表征,以评价其作为绿色萃取剂的潜力。测量了关键的物理化学性质,包括pH值、表面张力、极性和电导率。量子化学计算预测了组分之间有利的相互作用,并得到FTIR和质子核磁共振(¹H NMR)光谱的支持,证实了新氢键的形成。采用DES提取啤酒花球果中的天然化合物,并与异丙醇、水等常规溶剂进行比较。首次对“克隆18-11t”、“辉光”、“苦”、“芳香”和“十月”5个啤酒花品种的l -脯氨酸-甘油DES提取效率进行了评价。采用基于插值的优化方法减少实验工作量,提高资源效率。该算法使用IntelliJ IDEA Ultimate在Java中实现,数学计算使用Apache Commons和Apache POI,图形可视化使用JavaFX,为平滑的数据重构提供了一个健壮灵活的框架。研究了溶剂对啤酒花提取物中酚类化合物和黄酮类化合物含量及抗氧化活性的影响。通过电化学和分光光度法测定了啤酒花的抗氧化能力,发现啤酒花品种和溶剂体系对其抗氧化能力有显著影响。气相色谱-质谱分析表明,不同品种的苦味酸和芳香族化合物具有不同的特征。值得注意的是,与乙醇相比,DES提取物提供了更好的抗氧化酚类化合物保存。使用分析绿色度量方法(AGREE)对提取方法的环境可持续性进行了评估,确认DES系统是一种更环保的替代方案。这些发现证明了DES作为一种生态友好的有效溶剂从植物材料中提取生物活性化合物的可行性。
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引用次数: 0
Effect of exogenous protein on 3D printing and gelling properties of low-salt sliver carp surimi (Hypophthalmichthys molitrix) 外源蛋白对低盐鲢鱼鱼糜3D打印及胶凝性能的影响
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-11-05 DOI: 10.1016/j.fbp.2025.11.001
Jinsong Wu , Yaxing Yang , Feng Li , Hu Shi , Fanbin Kong , Juming Tang , Yang Jiao
Silver carp surimi exhibits weak gel properties, and traditional reliance on high-salt formulations to improve structural stability limits its application in low-salt foods due to associated health risks. 3D printing technology offers a novel approach for the precise shaping and structural reconstruction of low-salt surimi. This study innovatively introduced three structural modifiers—soy protein isolate (SPI), wheat gluten (WG), and whey protein isolate (WPI)—to construct a low-salt (1 % NaCl) surimi composite system, enhancing its printability and gel-forming capability. Results showed that the addition level of exogenous proteins had significant dose-dependent effects on the water-holding capacity (WHC), gel strength, and rheological properties of the surimi. Within the addition range of 4–6 %, all three exogenous proteins effectively improved the printing performance and gel quality of the surimi. Among them, the addition of 4 % SPI yielded the optimal results, significantly increasing gel strength by 4.1 % and WHC by 1.2 %, while conferring the best 3D printability, characterized by excellent extrusion consistency and structural stability. WG addition resulted in a rough and porous microstructure. WPI provided the least improvement under equivalent addition levels. Furthermore, excessive addition (≥8 %) induced protein over-aggregation and excessively high network cross-linking density, which conversely reduced WHC and caused printing filament fracture. This study systematically elucidates the mechanism by which exogenous proteins improve the 3D printing performance of low-sodium surimi by modulating the composite gel network structure. It provides theoretical support and formulation optimization strategies for developing low-sodium, high-quality surimi products for 3D printing applications.
鲢鱼鱼糜具有弱凝胶特性,传统上依赖高盐配方来提高结构稳定性,由于相关的健康风险,限制了其在低盐食品中的应用。3D打印技术为低盐鱼糜的精确成型和结构重建提供了一种新的方法。本研究创新性地引入大豆分离蛋白(SPI)、小麦面筋(WG)和乳清分离蛋白(WPI)三种结构改性剂,构建了低盐(1 % NaCl)鱼糜复合体系,提高了其可打印性和成胶性。结果表明,外源蛋白的添加水平对鱼糜的持水能力、凝胶强度和流变性能有显著的剂量依赖性。在4 ~ 6% %的添加范围内,3种外源蛋白均能有效提高鱼糜的打印性能和凝胶质量。其中,添加4 % SPI的效果最佳,凝胶强度提高了4.1 %,WHC提高了1.2 %,同时具有最佳的3D打印性能,具有优异的挤出一致性和结构稳定性。WG的加入导致了粗糙和多孔的微观结构。在同等添加水平下,WPI的改善最小。此外,过量添加(≥8 %)会导致蛋白质过度聚集和网络交联密度过高,反过来会降低WHC,导致打印长丝断裂。本研究系统阐明了外源蛋白通过调节复合凝胶网络结构提高低钠鱼米3D打印性能的机制。为开发低钠、高品质的3D打印鱼糜产品提供理论支持和配方优化策略。
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引用次数: 0
Experimental and numerical investigation into the cleaning of particulate soils based on swelling and adhesion behavior 基于膨胀与黏附特性的颗粒土净化实验与数值研究
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-11-03 DOI: 10.1016/j.fbp.2025.10.023
O. Barteld, S. Kricke, J.-P. Majschak, H. Köhler
Cleaning simulations are a tool for optimizing cleaning processes. They are based on models that take into account the soil and its interaction with the cleaning fluid. For pure particulate soils, the dominant cleaning mechanism is adhesive detachment. The fluid uptake and the resulting swelling of the particles have a decisive influence on their adhesive strength to the substrate, which consequently affects the cleaning process.
A particulate soil was characterized with respect to its geometric dimensions before and during swelling in water. The height increase was used to parameterize a three-dimensional finite element model (FEM). Subsequently, the FEM simulation delivered the water content at the soil-substrate-interface. Micromanipulation tests were conducted to determine the adhesive strength. A correlation between the latter two was implemented in a cleaning simulation to predict cleaning time, taking into account the variability of particle properties.
The presented approach offers a framework for extending cleaning simulations to particulate soils. However, validation through cleaning experiments is needed to verify its robustness and general validity.
清洗模拟是优化清洗过程的工具。它们基于考虑了土壤及其与清洗液相互作用的模型。对于纯颗粒土壤,主要的清洁机制是粘着剂的分离。流体的吸收和由此产生的颗粒膨胀对它们与基材的粘合强度有决定性的影响,从而影响清洗过程。颗粒土在水中膨胀前和膨胀过程中的几何尺寸特征。采用高度增量对三维有限元模型进行参数化。随后,有限元模拟给出了土-基质界面处的含水量。显微操作试验确定了粘接强度。考虑到颗粒性质的可变性,在清洗模拟中实现了后两者之间的相关性,以预测清洗时间。所提出的方法为将清洁模拟扩展到颗粒土壤提供了一个框架。然而,需要通过清洗实验来验证其鲁棒性和一般有效性。
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引用次数: 0
Green pathways to seaweed protein: a comparative techno-economic appraisal of pH shifting, MAE, UAE, and EAE 海藻蛋白的绿色途径:pH变化,MAE, UAE和EAE的比较技术经济评价
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-30 DOI: 10.1016/j.fbp.2025.10.021
Michael Edgardo Pérez-Roa, Loveille Jun Gonzaga, Roberto Lavecchia, Antonio Zuorro
Seaweeds are emerging as sustainable protein sources, yet large-scale extraction remains limited by high costs, low yields, and energy-intensive processing. This study presents a techno-economic and simplified environmental assessment of four green extraction routes for seaweed-protein recovery: pH shifting, microwave-assisted extraction, ultrasound-assisted extraction, and enzyme-assisted extraction, along with a hybrid pH-shifting + ultrasound configuration. Process models were developed from peer-reviewed data and current market information for Ulva biomass. Capital and operating costs, profitability indicators, energy use, and carbon emissions were evaluated under different product prices, protein recoveries, and drying options. Microwave-assisted extraction achieved the highest recovery (50 %) and lowest carbon intensity (10.8 kg CO₂ per kg protein). Drying dominated both cost and energy demand, representing more than two-thirds of total investment and electricity use. Replacing freeze- with oven-drying improved financial performance but increased emissions, while higher protein recovery enhanced both economic and environmental outcomes. The hybrid pH–ultrasound route offered a balanced compromise between efficiency and simplicity. This comparative appraisal provides the first integrated assessment of economic and environmental trade-offs across green extraction pathways, offering strategic insights for scaling seaweed protein production sustainably.

Tweetable abstract

Microwave-assisted and hybrid pH-shifting + ultrasound methods achieve the best balance between cost, efficiency, and environmental impact for seaweed-protein extraction.
海藻正在成为可持续的蛋白质来源,但大规模提取仍然受到高成本、低产量和能源密集型加工的限制。本研究提出了四种绿色海藻蛋白提取途径的技术经济和简化环境评估:pH转移、微波辅助提取、超声辅助提取和酶辅助提取,以及pH转移+ 超声混合配置。过程模型是根据同行评审的数据和Ulva生物质的当前市场信息开发的。在不同的产品价格、蛋白质回收率和干燥选项下,评估了资本和运营成本、盈利指标、能源使用和碳排放。微波辅助提取的回收率最高(50 %),碳强度最低(每公斤蛋白质10.8 kg CO₂)。干燥在成本和能源需求方面占主导地位,占总投资和用电量的三分之二以上。用烘箱干燥法取代冷冻法改善了财务绩效,但增加了排放,而更高的蛋白质回收率提高了经济和环境效益。混合ph -超声路线提供了效率和简单性之间的平衡折衷。这一比较评估首次对绿色提取途径的经济和环境权衡进行了综合评估,为可持续地扩大海藻蛋白生产规模提供了战略见解。摘要微波辅助和混合ph位移+ 超声方法在海藻蛋白提取的成本、效率和环境影响之间取得了最佳平衡。
{"title":"Green pathways to seaweed protein: a comparative techno-economic appraisal of pH shifting, MAE, UAE, and EAE","authors":"Michael Edgardo Pérez-Roa,&nbsp;Loveille Jun Gonzaga,&nbsp;Roberto Lavecchia,&nbsp;Antonio Zuorro","doi":"10.1016/j.fbp.2025.10.021","DOIUrl":"10.1016/j.fbp.2025.10.021","url":null,"abstract":"<div><div>Seaweeds are emerging as sustainable protein sources, yet large-scale extraction remains limited by high costs, low yields, and energy-intensive processing. This study presents a techno-economic and simplified environmental assessment of four green extraction routes for seaweed-protein recovery: pH shifting, microwave-assisted extraction, ultrasound-assisted extraction, and enzyme-assisted extraction, along with a hybrid pH-shifting + ultrasound configuration. Process models were developed from peer-reviewed data and current market information for <em>Ulva</em> biomass. Capital and operating costs, profitability indicators, energy use, and carbon emissions were evaluated under different product prices, protein recoveries, and drying options. Microwave-assisted extraction achieved the highest recovery (50 %) and lowest carbon intensity (10.8 kg CO₂ per kg protein). Drying dominated both cost and energy demand, representing more than two-thirds of total investment and electricity use. Replacing freeze- with oven-drying improved financial performance but increased emissions, while higher protein recovery enhanced both economic and environmental outcomes. The hybrid pH–ultrasound route offered a balanced compromise between efficiency and simplicity. This comparative appraisal provides the first integrated assessment of economic and environmental trade-offs across green extraction pathways, offering strategic insights for scaling seaweed protein production sustainably.</div></div><div><h3>Tweetable abstract</h3><div>Microwave-assisted and hybrid pH-shifting + ultrasound methods achieve the best balance between cost, efficiency, and environmental impact for seaweed-protein extraction.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 542-557"},"PeriodicalIF":3.4,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-stage ionic and microbiological purification process for producing drinking water from Aegean Sea water 用爱琴海海水生产饮用水的单级离子和微生物净化工艺
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-30 DOI: 10.1016/j.fbp.2025.10.019
Jülide Erkmen , Meltem Kizilca Çoruh , Rıdvan Yakut
This study presents a process developed for the simultaneous removal of both microbiological and ionic contaminants from Aegean Sea water to produce potable and domestic water. Electrodialysis was performed in continuous mode during the study. A cell design containing anion exchange, cation exchange, and bipolar membranes was used Three key operational parameters—temperature, electrical potential, and flow rate—were systematically varied in the experimental design. In these parameters, microbiological analyses such as Total Coliform, Enterococcus, Cl. Perfringens, Total viable count (TVC) at 22 °C and Total viable count at 36 °C were examined in the Aegean Sea water at certain time intervals. At the same time, changes in the concentrations of Ca+ 2, K+, Mg+2,Cl- and Na+ were examined. As a result, it was determined that the mass transfer rate for ions increased with increasing potential difference, flow rate and temperature and that there were no living organisms left after the first 10 min. Under the conditions of 12.5 V, 0.5 L/min, and 30 °C, the seawater was analyzed and treatment efficiencies of 98–99 % for Na⁺ and Cl⁻ ions and, 58–72 % treatment efficiencies for Mg+ and Ca+ ions were reached at 540th minute. The potential difference was found to be the most influential parameter affecting the process efficiency. The treated water met WHO international standards for both ionic composition and microbiological safety. The study is promising in terms of the separation of water containing both bacteria and ions in a single process without requiring additional processing in the face of future water scarcity.
本研究提出了一种同时去除爱琴海海水中的微生物和离子污染物以生产饮用水和生活用水的工艺。在研究过程中以连续模式进行电渗析。采用阴离子交换、阳离子交换和双极膜的电池设计,系统地改变了三个关键操作参数——温度、电位和流速。在这些参数中,微生物学分析如总大肠菌群、肠球菌、Cl。在一定时间间隔内检测爱琴海海水中产气荚膜菌、22°C时总活菌数(TVC)和36°C时总活菌数。同时检测了Ca+ 2、K+、Mg+2、Cl-和Na+浓度的变化。结果表明,离子的传质速率随着电位差、流速和温度的增加而增加,并且在前10 min min后没有生物残留。在12.5 V、0.5 L/min、30℃条件下,对海水进行分析,在540 min时,Na +和Cl⁻的处理效率为98 ~ 99 %,Mg+和Ca+的处理效率为58 ~ 72 %。发现电位差是影响工艺效率最大的参数。处理后的水符合世卫组织离子组成和微生物安全的国际标准。这项研究很有希望在一个过程中分离含有细菌和离子的水,而不需要额外的处理,以应对未来的水资源短缺。
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引用次数: 0
Upcycling bovine bone waste into hybrid collagen scaffolds for 3D bioprinting application and bone regeneration 将牛骨废物升级为混合胶原蛋白支架,用于3D生物打印应用和骨再生
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-28 DOI: 10.1016/j.fbp.2025.10.020
Mihriban Aydoğan-Gemici , Beyza Çay , Saeid Chobdar Rahim , Zehra Betül Ahi
Nowadays, with the increasing population, bovine bone waste obtained from the growing food industry has been evaluated and an environmentally sustainable hybrid bone graft material has been developed. Bioinks for 3D bioprinting applications were prepared by combining native collagen extracted from demineralized bovine bones with inorganic components such as hydroxyapatite, β-tricalcium phosphate and bioactive glass. Rheological tests as a result of the characterization of bioinks revealed that printability and thermal stability increased in mineral-added groups. In vitro bio-compatibility analyses of grid-structured scaffolds produced with extrusion-based 3D printing were performed with human bone marrow-derived mesenchymal stem cells; cell viability was evaluated with MTT and the cell counting kit-8 tests performed on days 1, 4 and 7. In addition, cell morphology was examined with phalloidin/4′,6-Diamidine-2′-phenylindole dihydrochloride staining. All mineral-added collagen scaffolds supported cell adhesion and proliferation, and cell viability and cytoskeletal organization were determined to be higher in groups containing hydroxyapatite and β-tricalcium phosphate. Transforming animal-derived waste into high value-added biomaterials is an innovative approach that can be applied in tissue engineering and supports environmental sustainability. The developed bioinks are promising candidates in 3D bioprinting technology to produce personalized bone grafts.
如今,随着人口的不断增长,人们对日益增长的食品工业中获得的牛骨废物进行了评估,并开发了一种环境可持续的杂交植骨材料。3D生物打印应用的生物墨水是将从脱矿牛骨中提取的天然胶原蛋白与无机成分(如羟基磷灰石、β-磷酸三钙和生物活性玻璃)结合在一起制备的。生物墨水特性的流变学测试结果表明,添加矿物质的基团增加了印刷性和热稳定性。以人骨髓间充质干细胞为材料,对挤压3D打印制备的网格结构支架进行体外生物相容性分析;在第1、4和7天用MTT和细胞计数试剂盒-8检测评估细胞活力。用phalloidin/4′,6-Diamidine-2′-phenylindole dihydrochloride染色法观察细胞形态。所有添加矿物质的胶原支架均支持细胞粘附和增殖,并且羟基磷灰石和β-磷酸三钙组的细胞活力和细胞骨架组织更高。将动物来源的废物转化为高附加值的生物材料是一种创新的方法,可以应用于组织工程并支持环境的可持续性。所开发的生物墨水是3D生物打印技术中生产个性化骨移植物的有希望的候选者。
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引用次数: 0
Control strategies of polar and non-polar phytosterol derivatives in the chemical synthesis process of phytosterol esters 植物甾醇酯化学合成过程中极性和非极性植物甾醇衍生物的控制策略
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-28 DOI: 10.1016/j.fbp.2025.10.022
Jie Mi , Si Chen , Qidong Xue , Peng Zhang , Zhengzhang Wu , Ruijie Liu , Ming Chang
Phytosterol esters (PE) is a useful dietary supplement. However, oxidation and dehydration reactions during chemical synthesis would reduce product quality and harm human health. In order to improve the quality of PE, firstly, 13 commercially PE were collected (9 chemical synthesis, 4 enzymatic synthesis). The results showed that polar phytosterol derivatives (POPs) in 2 products and non-polar phytosterol derivatives (DS) in 6 products exceeded 1000.00 mg/kg. The dominant POPs were 7β-OH, 7-keto, 5β,6β-epoxy, and 24,25-epoxy, and 3,5-sterene was the main structure in DS. The accumulation of POPs and DS reduced the quality of PE. Then, it was found that oxygen significantly affected 2,4,6-sterene and POPs (P < 0.01). Reaction time, temperature and fatty acids unsaturation had significantly affected esterification rate and dehydration rate (P < 0.01). At last, the optimal synthesis process was researched using the Box-Behnken design with an esterification rate of 91.87 % and a dehydration rate of 0.72 %.
植物甾醇酯(PE)是一种有用的膳食补充剂。然而,化学合成过程中的氧化和脱水反应会降低产品质量,危害人体健康。为了提高聚乙烯的质量,首先收集了13种市售聚乙烯(化学合成9种,酶促合成4种)。结果表明,2种产品的极性植物甾醇衍生物(pop)和6种产品的非极性植物甾醇衍生物(DS)含量均超过1000.00 mg/kg。持久性有机污染物主要为7β-OH、7-酮、5β、6β-环氧和24,25-环氧,其中3,5-甾烯为主要结构。POPs和DS的积累降低了PE的质量。然后,发现氧对2,4,6-甾烯和持久性有机污染物的影响显著(P <; 0.01)。反应时间、温度和脂肪酸不饱和度对酯化率和脱水率有显著影响(P <; 0.01)。最后,采用Box-Behnken设计优化合成工艺,酯化率为91.87 %,脱水率为0.72 %。
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引用次数: 0
YOLO-based classification algorithm for irregular and occluded Bonnet bellflower roots (Codonopsis lanceolata): Optimizing thermal processing through image extraction, clustering, and CFD simulation 基于yolo的风头草不规则和闭塞根(Codonopsis lanceolata)分类算法:通过图像提取、聚类和CFD模拟优化热处理
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-25 DOI: 10.1016/j.fbp.2025.10.018
Timilehin Martins Oyinloye , Won Byong Yoon
This study proposes a novel system for optimizing the steaming process of Bonnet balloon flower (Codonopsis lanceolata) roots by integrating YOLO-based object detection, image analysis, K-means clustering, and computational fluid dynamics (CFD) simulations. It focuses on size classification for determining optimal thermal processing conditions, with YOLO-based object detection offering an innovative approach to accurately, non-destructively sorting irregularly shaped roots into distinct classes. Roots were categorized into three classes (0, 1, and 2) based on morphology, with average lengths of 297.64 ± 31.34, 152.67 ± 29.87, and 189.17 ± 23.45 mm, and thicknesses of 37.73 ± 2.24, 28.89 ± 2.37, and 29.67 ± 2.77 mm, respectively. YOLOv12 achieved high accuracy, with Intersection over Union (IoU) values of 0.999 (well-arranged) and 0.953 (occluded), even under complex conditions. Classified roots, particularly in classes 1 and 2, exhibited lower internal crack rates (47.36 ± 2.59 % and 32.87 ± 2.45 %) and more uniform reductions in total dissolved solids (TDS) compared to non-classified roots. Steaming for 40 min at 100°C ensured microbial safety, while smaller roots (class 1 and 2) reached thermal equilibrium faster (45.78–51.22 min) due to efficient heat transfer. The system’s integration of YOLO-based classification presents a major advancement in optimizing thermal processing of irregularly shaped plant roots by enabling rapid sorting and improving product consistency.
本研究提出了一种基于yolo的目标检测、图像分析、K-means聚类和计算流体动力学(CFD)模拟相结合的球囊花(Codonopsis lanceolata)根蒸过程优化系统。它专注于尺寸分类,以确定最佳的热处理条件,基于yolo的物体检测提供了一种创新的方法,可以准确、非破坏性地将不规则形状的根分类为不同的类别。根被分为三个类(0,1,2)基于形态学,平均长度为297.64 ± 31.34,152.67 ± 29.87,和189.17 ±23.45  mm,不同厚度的37.73 ± 2.24,28.89 ± 2.37,和29.67 ±2.77  mm,分别。即使在复杂条件下,YOLOv12也取得了很高的精度,在排列良好的情况下,IoU值为0.999,在遮挡的情况下,IoU值为0.953。分类根系,特别是分类1和分类2的根系,与未分类根系相比,表现出更低的内部裂纹率(47.36 ± 2.59 %和32.87 ± 2.45 %)和更均匀的总溶解固形物(TDS)减少。在100°C下蒸40 min确保微生物安全,而较小的根(1类和2类)由于有效的传热,更快达到热平衡(45.78-51.22 min)。该系统集成了基于yolo的分类,通过实现快速分类和提高产品一致性,在优化不规则形状植物根系的热处理方面取得了重大进展。
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引用次数: 0
Dielectric properties of black soldier fly larvae at 2.45 GHz, and their dependence with moisture content, temperature, or bulk density 黑兵蝇幼虫在2.45 GHz下的介电特性及其与含水量、温度和容重的关系
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-25 DOI: 10.1016/j.fbp.2025.10.013
J. Fayos-Fernández , R. Pérez-Campos , J. Monzó-Cabrera , M. Nowakowski , J. Majerska , A. Zimmer
As the global population continues to rise, the demand for traditional food sources, especially animal protein, intensifies, leading to increasing scarcity. The black soldier fly (BSF) larvae are an alternative high protein source (up to 44 %) which farming is environmentally sustainable. This contribution aims to increase the industrial processing efficiency by examining the feasibility of using microwave technology at the right larvae instar for its storage. Microwave processing offers advantages such as rapid water removal, nutritional preservation, and microbial reduction. This paper characterizes experimentally the dielectric constant (ε′) and loss factor (ε′′) of BSF larvae, and their dependency with temperature, moisture content (MC), and bulk density (BD), to enable optimized microwave drying systems. Results show that the permittivity varies significantly with the MC. The decrease of MC, the goal of any drying process, is linked to the BD reduction, and this physical parameter has even a stronger influence on the permittivity. Finally, the temperature increase affects the loss factor, causing an increase of the heating speed. Nevertheless, all these magnitudes combined keeps the larvae dielectric parameters within a range that provides insights for improving the microwave drying efficiency of processed BSF larvae at an industrial scale.
随着全球人口的持续增长,对传统食物来源,特别是动物蛋白的需求增加,导致日益稀缺。黑兵蝇(BSF)幼虫是另一种高蛋白来源(高达44% %),养殖在环境上是可持续的。本论文旨在通过研究微波技术在合适的幼虫龄期进行储存的可行性,提高工业加工效率。微波处理具有快速除水、营养保存和微生物减少等优点。为了优化微波干燥系统,本文通过实验研究BSF幼虫介电常数(ε′)和损耗因子(ε′)及其与温度、含水量(MC)和容重(BD)的关系。结果表明,介质介质的介电常数随介质介质密度的变化有显著的变化,任何干燥过程的目标都是降低介质介质密度,而介质介质密度对介电常数的影响更大。最后,温度的升高影响了损耗因子,导致加热速度的增加。然而,所有这些数值结合起来,使幼虫的介电参数保持在一个范围内,这为提高工业规模上加工过的BSF幼虫的微波干燥效率提供了见解。
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Food and Bioproducts Processing
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