Food and Bioproducts Processing最新文献_第5页

Effect of pulsed light on enzyme inactivation, colour, firmness, surface morphology, and bioactive compounds in fresh and dried whole red chilies (Capsicum annuum var. longum) 脉冲光对新鲜和干红辣椒（Capsicum annuum var. longum）中酶失活、颜色、硬度、表面形态和生物活性化合物的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-10-11 DOI: 10.1016/j.fbp.2024.10.005

Kosana Pravallika, Snehasis Chakraborty

The effect of pulsed light on enzymatic activity and biochemical stability of red chillies was investigated. Balancing and reducing the a_w and moisture content to 0.6 a_w and 0.35 a_w is crucial to attain biochemical stability. Upon drying at 60 °C/5 h + 40 °C/2 h and 70 °C/11 h + 55 °C/4 h + 40 °C/2 h the enzymes inactivated to more than 30 %, respectively. Hence, to completely inactivate the enzymes, a technology has been introduced i.e., pulsed light treatment (PLT), which employed 0.53–2.59 J·cm⁻². The stability of red chillies was evaluated based on the quality attributes such as enzyme activity, total phenolics, flavonoids, antioxidant capacity, ascorbic acid, capsiacinoids, and carotenoids. After treatment at fluence of 2.59 J·cm⁻², there was >90 % inactivation of PPO and POD. Based on kinetic modelling, it is showed PLT better preserved the colour, texture, morphology, and bioactive compounds in 0.6 and 0.35 a_w than 0.85 a_w red chillies. Morphological studies determined the changes in red chillies after PLT. FTIR and GCMS analysis revealed the structural and compositional changes occurring after PLT. Therefore, drying combined with PL can be a potential alternative method to blanching along with maximizing the availability of bioactives.

研究了脉冲光对红辣椒酶活性和生化稳定性的影响。要达到生化稳定性，关键是要平衡并将 aw 和水分含量降低到 0.6 aw 和 0.35 aw。在 60 °C/5 h + 40 °C/2 h 和 70 °C/11 h + 55 °C/4 h + 40 °C/2 h 下干燥时，酶的失活程度分别超过 30%。因此，为了彻底灭活酶，引入了一种技术，即脉冲光处理（PLT），使用 0.53-2.59 J-cm-2。根据酶活性、总酚类、类黄酮、抗氧化能力、抗坏血酸、辣椒素和类胡萝卜素等质量属性，对红辣椒的稳定性进行了评估。在 2.59 J-cm-2 的荧光下处理后，PPO 和 POD 的失活率为 90%。根据动力学模型，与 0.85 aw 的红辣椒相比，0.6 aw 和 0.35 aw 的 PLT 能更好地保持红辣椒的颜色、质地、形态和生物活性化合物。形态学研究确定了 PLT 后红辣椒的变化。傅立叶变换红外光谱（FTIR）和气相色谱-质谱（GCMS）分析显示了 PLT 后结构和成分的变化。因此，干燥结合 PLT 是一种替代焯水的潜在方法，同时还能最大限度地提高生物活性物质的可用性。

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引用次数: 0

A combined-sweeping & complementary-relative-phase strategy to improve heating performance in a dual-port solid-state microwave system 改善双端口固态微波系统加热性能的扫频和互补相对相位组合策略

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-10-11 DOI: 10.1016/j.fbp.2024.10.007

Arjun Ghimire, Jiajia Chen

Dual-port solid-state microwave heating could address the single-port low microwave power issue and potentially result in better microwave heating performance. This study developed and evaluated a combined-sweeping & complementary-relative-phase-shifting heating strategy for dual-port microwave heating of foods. The combined-sweeping & complementary-relative-phase-shifting strategy first orderly swept the relative phases between two ports from 0° to 315° at an interval of 45° operated at a frequency of 2.45 GHz and a power level of 200 W for each port to determine relative phase-dependent thermal contributions. Then, relative phases that have complementary thermal contributions to the real-time heating results were determined and used in the heating process. The performance of microwaving a tray of 300 g gellan gel model food for 3 minutes was compared with that of the fixed-relative-phase and orderly-sweeping-relative-phase strategies. The results showed that the complementary strategy delivered significantly more uniform heating results (HUI = 0.27 ± 0.01) than the other strategies. Similar microwave power absorption and maximum and minimum temperatures were observed in the complementary and orderly sweeping heating strategies, which was better than the fixed heating strategy. Overall, the complementary strategy showed superior heating uniformity, followed by the orderly sweeping and then the fixed-relative-phase heating strategy. The complementary relative phase shifting strategy is promising to be incorporated into solid-state microwave systems for smart heating processes.

双端口固态微波加热可以解决单端口微波功率低的问题，并可能带来更好的微波加热性能。这项研究开发并评估了一种用于食品双端口微波加热的联合扫频& 互补相对移相加热策略。组合-扫描& 互补-相对相移策略首先有序地扫描两个端口之间的相对相位，从 0°到 315°，间隔 45°，工作频率为 2.45 GHz，每个端口的功率水平为 200 W，以确定与相对相位相关的热贡献。然后，确定了对实时加热结果具有互补热贡献的相对相位，并将其用于加热过程。将一盘 300 克的结冷胶模型食品微波 3 分钟的性能与固定相对相位策略和有序扫动相对相位策略进行了比较。结果表明，互补策略的加热均匀度（HUI = 0.27 ± 0.01）明显高于其他策略。在互补和有序扫描加热策略中，观察到了相似的微波功率吸收以及最高和最低温度，这优于固定加热策略。总的来说，互补策略的加热均匀性更好，其次是有序扫描策略，然后是固定相对移相加热策略。互补相对相移策略有望被纳入固态微波系统，用于智能加热过程。

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引用次数: 0

Beetroot ketchup as a stable carrier of potential probiotic Lacticaseibacillus rhamnosus K3 and Lactobacillus johnsonii K4: A study on sensory attributes, storage viability, and in vitro gastrointestinal survival 甜菜根番茄酱作为潜在益生菌鼠李糖乳杆菌 K3 和约翰逊乳杆菌 K4 的稳定载体：感官属性、储存活力和体外胃肠道存活率研究

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-10-10 DOI: 10.1016/j.fbp.2024.10.004

Kübra Küçükgöz , Koen Venema , Monika Trząskowska

This research aimed to assess the viability of Lacticaseibacillus rhamnosus K3 and Lactobacillus johnsonii K4 in beetroot ketchup during storage and simulated digestion to examine fermentation effects on sensory quality. The findings revealed that both strains maintained viability above 8 log₁₀ CFU/ml during storage, confirming their potential as probiotics. pH levels changed significantly over three-week storage period indicating fermentation's impact on shelf stability. The control sample maintained consistent pH level of 4.6, while pH of ketchup fermented with L. rhamnosus K3 decreased from 3.84 to 3.79, and ketchup fermented with L. johnsonii K4 decreased from 3.96 to 3.69. Sensory evaluations showed statistically significant differences in odor, texture, flavor, and overall quality between samples. Fermentation with L. johnsonii K4 improved sensory attributes, achieving the highest overall quality score with mean value of 7.31 out of 10, compared to 6.28 for the control and 6.23 for the L. rhamnosus K3 fermented ketchup. Survival rate of L. johnsonii K4 was 27 %, compared to 2.8 % for L. rhamnosus K3 in dynamically simulated gastrointestinal system TIM-1. Both fermented ketchups contained over 10⁹ CFU of viable cells. These results demonstrate that plant-based food products can effectively serve as carriers for potential probiotic strains, preserving their viability during storage and digestion, while enhancing sensory quality of food products.

这项研究旨在评估甜菜根番茄酱中的鼠李糖乳杆菌 K3 和约翰逊乳杆菌 K4 在储存和模拟消化期间的活力，以考察发酵对感官质量的影响。研究结果表明，这两种菌株在贮藏期间的存活率都保持在 8 log10 CFU/ml 以上，证实了它们作为益生菌的潜力。在三周的贮藏期内，pH 值发生了显著变化，表明发酵对货架稳定性产生了影响。对照样品的 pH 值始终保持在 4.6，而用鼠李糖 K3 发酵的番茄酱的 pH 值从 3.84 降至 3.79，用约翰逊酵母 K4 发酵的番茄酱的 pH 值从 3.96 降至 3.69。感官评估显示，不同样品在气味、质地、风味和总体质量方面存在显著的统计学差异。用约翰逊酵母 K4 发酵的番茄酱改善了感官属性，总体质量得分最高，平均值为 7.31（满分 10 分），而对照组为 6.28，鼠李糖 K3 发酵的番茄酱为 6.23。在动态模拟胃肠道系统 TIM-1 中，约翰逊酵母 K4 的存活率为 27%，鼠李糖 K3 的存活率为 2.8%。两种发酵番茄酱都含有超过 109 CFU 的存活细胞。这些结果表明，植物性食品可以有效地作为潜在益生菌株的载体，在储存和消化过程中保持其活力，同时提高食品的感官质量。

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引用次数: 0

Pilot-scale protein extraction of green seaweed (Ulva spp.) whole biomass and pulp – Investigating biochemical composition and protein digestibility in a rat trial 试点规模的绿海藻（莼菜属）全生物质和纸浆蛋白质提取--在大鼠试验中调查生化成分和蛋白质消化率

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-10-09 DOI: 10.1016/j.fbp.2024.10.003

Signe H. Nissen , Louise Juul , Lene Stødkilde , Annette Bruhn , Morten Ambye-Jensen , Trine K. Dalsgaard

Ulva, a green seaweed, is gaining interest for food applications due to its high content of protein and well-balanced amino acid composition. However, protein extraction is necessary to reduce the ash content and increase the protein digestibility. In this study, pilot scale protein extraction from Ulva was obtained by a mechanical fractionation, creating a pulp and a juice. A second extraction of protein from the pulp was obtain after a pH shift to 8.5 and the proteins from the two juices were acid precipitated with and without a clearing step (decanting of the resulting juice). Extraction of protein from the whole biomass resulted in a higher content of crude protein (CP) (31.8–37.6 %), essential amino acids (EAA) (42.4 %), and nitrogen digestibility (59.5–63.3 %) than from the pulp (26.1–29.5 % CP of DM, 40.1–40.3 % EAA, 47.4±3.6 % nitrogen digestibility). Including the clearing step increased the protein content but not the nitrogen digestibility (59.5±4.8 % with clearing vs 63.3±3.4 % without). None of the extractions induced cross-linked AA of concern. However, elements were concentrated, where concentrations of arsenic might limit the utilization in food. These results indicate that upscaling of the protein extraction from Ulva is promising, but further improvement regarding toxic elements and protein digestibility is necessary.

莼菜是一种绿色海藻，因其蛋白质含量高、氨基酸组成均衡而越来越多地被应用于食品领域。然而，要降低灰分含量并提高蛋白质的消化率，就必须进行蛋白质提取。在这项研究中，通过机械分馏从莼菜中提取蛋白质，形成了浆和汁。在将 pH 值调至 8.5 后，对果肉中的蛋白质进行第二次萃取，并对两种果汁中的蛋白质进行酸沉淀，包括清汁步骤和不清汁步骤（倾析所得果汁）。从整个生物质中提取蛋白质后，粗蛋白（CP）含量（31.8-37.6%）、必需氨基酸（EAA）含量（42.4%）和氮消化率（59.5-63.3%）均高于从果肉中提取的蛋白质（DM 的 CP 含量为 26.1-29.5%，EAA 含量为 40.1-40.3%，氮消化率为 47.4±3.6%）。清浆步骤提高了蛋白质含量，但没有提高氮消化率（清浆后为 59.5±4.8 %，未清浆为 63.3±3.4%）。所有萃取方法都不会引起值得关注的交联 AA。然而，元素被浓缩，其中砷的浓度可能会限制其在食物中的利用。这些结果表明，提高从石莼（Ulva）中提取蛋白质的水平是有希望的，但有必要进一步改善有毒元素和蛋白质消化率。

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引用次数: 0

Gelatin-crosslinked millard reaction products of sodium caseinate/ maltodextrin enhances the encapsulation efficiency and stability of krill oil microcapsules 明胶交联酪蛋白酸钠/麦芽糊精的米拉德反应产物可提高磷虾油微胶囊的封装效率和稳定性

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-10-02 DOI: 10.1016/j.fbp.2024.10.001

Yating Zhang , Kailiang Leng , Xixi Wang , Zipeng Zhang , Dong Su , Ling Zhao , Xiaofang Liu , Yuan Yu , Junkui Miao , Yueqin Yu

The Maillard reaction is an effective way to enhance the properties of microcapsule wall materials. This study investigated the optimal ratio and physicochemical properties of Maillard reaction products from sodium caseinate (CAS) and maltodextrin (MD), as well as their crosslinked derivatives with gelatin (GEL). The application of Maillard reaction products (CAMD) as wall materials in the preparation of krill oil microcapsules (KOMs), raised the encapsulation efficiency (EE) of KOMs to 94.5 % compared with the physical mixture. The further addition of gelatin raised the EE to 96.9 %. The gelatin crosslinked Maillard reaction products (CAMEL) also exhibited superior antioxidative properties, and as wall materials enhanced the storage stability of KOMs, whose surface oil was 16.98 % over six months, only 71.16 % of that of the physical mixture. In vitro simulated release of KOMs demonstrated that CAMEL could reduce the release of free fatty acids (FFA) by 14.66 % compared to physical mixtures. These findings suggest that microencapsulation with CAMEL provides enhanced protection for krill oil, demonstrating significant potential for the practical application in the scale-up production.

马氏反应是提高微胶囊壁材料性能的有效方法。本研究调查了酪蛋白酸钠（CAS）和麦芽糊精（MD）的马氏反应产物以及它们与明胶（GEL）的交联衍生物的最佳配比和理化性质。在磷虾油微胶囊（KOMs）的制备过程中，使用麦拉德反应产物（CAMD）作为壁材，与物理混合物相比，KOMs 的封装效率（EE）提高到 94.5%。进一步添加明胶后，EE 提高到 96.9%。明胶交联麦拉德反应产物（CAMEL）还具有优异的抗氧化性能，作为壁材增强了KOMs的储存稳定性，其表面油分在六个月内为16.98%，仅为物理混合物的71.16%。KOMs 的体外模拟释放表明，与物理混合物相比，CAMEL 可将游离脂肪酸（FFA）的释放量减少 14.66%。这些研究结果表明，CAMEL 微胶囊技术可加强对磷虾油的保护，在扩大生产规模的实际应用中具有巨大潜力。

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引用次数: 0

Determination of phenolics and proteins causing sediment formation in pomegranate juice using “Tannase+Lactonase” and “Protease” 使用 "单宁酶+乳酸菌酶 "和 "蛋白酶 "测定石榴汁中导致沉淀形成的酚类物质和蛋白质

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-10-02 DOI: 10.1016/j.fbp.2024.09.022

Meltem Türkyılmaz , Fatmagül Hamzaoğlu , Mehmet Özkan

In this study, pomegranate juice (PJ) was treated with “tannase+lactonase [human-derived (H-PON-1) or rabbit-derived (R-PON-1)]” or “protease [aspartic protease (AP) or cysteine protease (CP)].” After treatment, effects of tannins, proteins and amino acids on sediment formation were determined during storage at 20℃. Among proteins, prolamin showed the highest effect in PJ treated with “tannase+H-PON-1/R-PON-1” and CP. While amino acids did not affect sediment formation in “tannase+H-PON-1/R-PON-1” or AP treatments, only glycine increased sediment content in CP treatment. Removal of galloyl and/or HHDP groups from HHDP-galloyl-glucose-isomer, pedunculagin-2 and punigluconin by “tannase+H-PON-1/R-PON-1” was very effective in preventing sediment formation. Prolamin:galloyl glucose isomer ratio and HHDP-galloyl-hexoside-2 and HHDP-galloyl-glucuronide contents should be 23.244±1.162 and, 1.254±0.063 and 0.679±0.034 mg/L to prevent sediment, respectively. Since common parameters preventing sediment formation in both methods that target removal of tannins and/or proteins were punigluconin content and prolamin:punicalagin-2 ratio, these two parameters must also be considered in sediment-free PJ production.

在这项研究中，石榴汁（PJ）用 "单宁酸酶+乳酸菌酶[人源性（H-PON-1）或兔源性（R-PON-1）]"或 "蛋白酶[天冬氨酸蛋白酶（AP）或半胱氨酸蛋白酶（CP）]"处理。处理后，测定单宁酸、蛋白质和氨基酸在 20℃储存期间对沉淀物形成的影响。在蛋白质中，用 "单宁酶+H-PON-1/R-PON-1 "和 CP 处理的 PJ 中，丙烯胺的影响最大。氨基酸不影响 "单宁酶+H-PON-1/R-PON-1 "或 AP 处理中沉淀物的形成，而只有甘氨酸会增加 CP 处理中的沉淀物含量。用 "单宁酶+H-PON-1/R-PON-1 "去除 HHDP-galloyl-glucose-isomer、pedunculagin-2 和 punigluconin 中的五倍子酰基和/或 HHDP 基团对防止沉积物形成非常有效。前胡素：五倍子葡萄糖异构体比、HHDP-五倍子己糖苷-2 和 HHDP-五倍子葡萄糖醛酸含量应分别为 23.244±1.162、1.254±0.063 和 0.679±0.034 mg/L，才能防止泥沙形成。由于在这两种以去除单宁和/或蛋白质为目标的方法中，防止沉淀物形成的共同参数是punigluconin含量和prolamin:punicalagin-2比率，因此在无沉淀PJ生产中也必须考虑这两个参数。

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引用次数: 0

Isoflavone-rich extracts from okara using supercritical fluid extraction: Kinetic modeling and characterization 利用超临界流体萃取技术从秋葵中提取富含异黄酮的提取物：动力学建模与表征

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-28 DOI: 10.1016/j.fbp.2024.09.019

Elixabet Díaz-de-Cerio , Pedro Barea , Rodrigo Melgosa , Alba Ester Illera , María Teresa Sanz , Sagrario Beltrán

The lipid fraction and bioactive compounds from okara, a by-product of soybean processing, have been extracted by supercritical fluid extraction. The effect of operating parameters, pressure (20–40 MPa), temperature (40–80 ºC), and cosolvent usage (0–10 %wt. ethanol), on the extraction kinetics has been investigated, satisfactorily correlating the experimental data to the Sovová’s model. Extraction yield by SFE ranged from 0.1014 g extract/g insoluble solid (IS) at 20 MPa and 80 ºC to 0.1081 g extract/g IS at 30 MPa and 60 ºC (from 9.2 to 9.8 g extract/100 g okara, respectively), similar to Soxhlet extraction with n-hexane (9.2 ± 0.2 g extract/100 g). Pressure positively affected the initial extraction rate, which also increased with temperature except for the lowest pressure of 20 MPa, exhibiting a typical cross-over behavior. The use of ethanol as cosolvent promoted the extraction of phenolic compounds and isoflavones, and was less important in the extraction of tocopherols. Extraction yield using ethanol increased up to 0.1100 g extract/g IS at 40 MPa and 40 ºC. As expected, the higher content of bioactive compounds in the sc-CO₂ + ethanol extracts positively affected the antioxidant capacity, with the maximum found at 20 MPa, 40 ºC and 10 %wt. ethanol.

采用超临界流体萃取法萃取了大豆加工副产品黄卡拉中的脂质部分和生物活性化合物。研究了压力（20-40 兆帕）、温度（40-80 ºC）和共溶剂用量（0-10 % 重量百分比乙醇）等操作参数对萃取动力学的影响，实验数据与索沃娃模型的相关性令人满意。在20兆帕和80摄氏度时，SFE的萃取率为0.1014克萃取物/克不溶性固体（IS）；在30兆帕和60摄氏度时，萃取率为0.1081克萃取物/克不溶性固体（每100克黄原胶的萃取率分别为9.2克到9.8克），与使用正己烷的索氏萃取（9.2 ± 0.2克萃取物/100克）相似。压力对初始萃取率有积极影响，除了最低压力为 20 兆帕外，萃取率也随温度升高而增加，表现出典型的交叉行为。使用乙醇作为助溶剂可促进酚类化合物和异黄酮的萃取，但对生育酚的萃取影响较小。在 40 兆帕和 40 ºC 条件下，乙醇的萃取率最高可达 0.1100 克萃取物/克 IS。不出所料，sc-CO2 + 乙醇提取物中生物活性化合物含量较高，对抗氧化能力产生了积极影响，在 20 兆帕、40 ºC 和 10 %wt.

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引用次数: 0

Technical-economic assessment of 5-hydroxymethylfurfural production via catalytic hydrothermal synthesis from lignocellulosic forest residues 利用木质纤维素森林残留物进行催化水热合成生产 5-羟甲基糠醛的技术经济评估

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-27 DOI: 10.1016/j.fbp.2024.09.021

Tiago Guimarães, Ricardo de Carvalho Bittencourt, Ana Márcia Macedo Ladeira Carvalho, Sebastião Renato Valverde, Marcelo Moreira da Costa

The 5-hydroxymethylfurfural is a high-value chemical that can be used as a raw material for the synthesis of biofuels and bioplastics. The use of forest woody residues as a source of biomass reduces the costs and environmental impacts associated with the process. The objective of this study was to optimize the conditions of catalytic hydrothermal synthesis of 5-HMF from the residue of three biomasses, and to carry out a technical-economic assessment of the feasibility of the process on an industrial scale. The materials used were forest woody residues, distilled water, acetic acid (CH₃COOH), and niobium oxide (Nb2O5) as a reaction catalyst.The identification and quantification of the 5-HMF yield was monitored by high-performance liquid chromatography (HPLC). For the technical-economic assessment, the CAPCOST Estimation Software® was used, which allows estimating the capital and operation costs, as well as the financial indicators of the project. Under optimized conditions, the maximum yield of 5-HMF was 32.6 %, based on dry mass (bdm). The technical-economic assessment (TEA) indicated that the project is economically viable, considering a selling price of 5-HMF of 2.16 dollars/kg. The main financial indicators were CAPEX of 197.83 million dollars, OPEX of 99.78 million dollars, net present value (NPV) of 197.83 million dollars, internal rate of return (IRR) of 44 %, and the payback time of 2.25 years.

5- 羟甲基糠醛是一种高价值化学品，可用作合成生物燃料和生物塑料的原料。使用森林木质残渣作为生物质来源可降低该工艺的成本和对环境的影响。本研究旨在优化从三种生物质残渣中催化水热合成 5-HMF 的条件，并对该工艺在工业规模上的可行性进行技术经济评估。使用的材料为森林木质残留物、蒸馏水、乙酸（CH3COOH）和作为反应催化剂的氧化铌（Nb2O5）。在进行技术经济评估时，使用了 CAPCOST Estimation Software®，该软件可以估算资本和运营成本，以及项目的财务指标。在优化条件下，按干重（bdm）计算，5-HMF 的最高产量为 32.6%。技术经济评估（TEA）表明，考虑到 5-HMF 的销售价格为 2.16 美元/公斤，该项目在经济上是可行的。主要财务指标为：资本支出（CAPEX）1.9783 亿美元，运营支出（OPEX）9978 万美元，净现值（NPV）1.9783 亿美元，内部收益率（IRR）44%，投资回收期 2.25 年。

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引用次数: 0

Effect of production scale on the techno-economic viability and environmental life cycle analysis of lactic acid production in a sugarcane biorefinery 生产规模对甘蔗生物精炼厂乳酸生产的技术经济可行性和环境生命周期分析的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-27 DOI: 10.1016/j.fbp.2024.09.020

Mensah S. Brobbey, Janus P. Louw, Jeanne Louw, Johann F. Görgens

Biorefineries are vital for advancing circular economy and reducing the effects of products fossil fuels derived products on the environment. However, biorefineries often operate at smaller production scales than fossil refineries due to limited feedstock availability, which may be addressed by centralised processing with multiple feedstock sources. Lactic acid (LA) has several industrial applications and is a platform chemical used to produce products like acrylic acid and propylene glycol. The economic and environmental performances of an integrated biorefinery at different production scales, through different levels of feedstock centralization, were investigated, to determine the optimal scale for sugarcane-based LA production. Decreasing values for the minimum selling price (MSP; 1312–849 US$.t⁻¹) and increasing internal rates of return (IRRs; 31–64 %) were observed with increasing conversion scales of sugarcane A-molasses. The MSPs decrease from 90 to 450 kt_LA.y⁻¹ with small improvements in profitability beyond 450 kt_LA.y⁻¹, as confirmed by stochastic financial uncertainty analysis. In the environmental assessment, a linear increase was observed across all impact categories, mainly due to the added fuel consumption for feedstock transportation. LA production had a GWP100 range of 0.87–0.95 kg CO₂-eq.kg_LA⁻¹ and an abiotic depletion potential of 12–13 MJ.kg_LA⁻¹ which increased as the scale increased. In the ozone depletion category emissions of 9.96×10⁻⁸-1.13×10⁻⁷ kg CFC-11 eq.kg_LA⁻¹ comparable to other studies available in literature. Similarly, emission ranges of 1.11–1.17, 0.63–0.66, and 1581–1641 kg 1,4-DB eq.kg_LA⁻¹ were obtained in the human toxicity, freshwater and marine aquatic ecotoxicity categories as the scale increased. Environmentally the smallest scale at which transportation of feedstock was avoided (i.e. 90 kt_LA.y⁻¹) is preferred as opposed to 450 kt_LA.y⁻¹ for economic performance.

生物精炼厂对于推进循环经济和减少化石燃料衍生产品对环境的影响至关重要。然而，由于原料供应有限，生物精炼厂的生产规模往往小于化石精炼厂，这可以通过多种原料来源的集中加工来解决。乳酸（LA）有多种工业用途，是一种用于生产丙烯酸和丙二醇等产品的平台化学品。通过不同程度的原料集中化，研究了不同生产规模的综合生物精炼厂的经济和环境性能，以确定以甘蔗为原料的 LA 生产的最佳规模。随着甘蔗 A-蔗糖转化规模的扩大，最低销售价格（MSP；1312-849 美元/吨-1）不断降低，内部收益率（IRR；31-64%）不断提高。随机财务不确定性分析证实，从 90 ktLA.y-1 到 450 ktLA.y-1，最大生产潜力下降，超过 450 ktLA.y-1 后盈利能力略有提高。在环境评估中，所有影响类别都呈线性增长，这主要是由于原料运输增加了燃料消耗。LA 生产的 GWP100 范围为 0.87-0.95 kg CO2-eq.kgLA-1，非生物消耗潜能值为 12-13 MJ.kgLA-1，随着规模的扩大而增加。臭氧消耗类的排放量为 9.96×10-8-1.13×10-7 kg CFC-11eq.kgLA-1，与文献中的其他研究结果相当。同样，随着规模的增加，在人类毒性、淡水和海洋水生生态毒性类别中，排放范围分别为 1.11-1.17、0.63-0.66 和 1581-1641 kg 1,4-DB eq.kgLA-1。从环境角度看，避免原料运输的最小规模（即 90 ktLA.y-1）比 450 ktLA.y-1 更符合经济效益。

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引用次数: 0

Impact of alkaline solution, carbohydrase and biomass:solvent on extraction efficiency, and protein and amino acid profiles of Palmaria palmata protein extracts 碱性溶液、碳水化合物酶和生物质：溶剂对棕榈蛋白提取物的提取效率以及蛋白质和氨基酸谱的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2024-09-26 DOI: 10.1016/j.fbp.2024.09.017

Anthony Temitope Idowu , Miryam Amigo-Benavent , Richard.J. FitzGerald

The impact of using different alkaline solutions, carbohydrases and changing the biomass to solvent volume ratio (biomass:solvent) on protein content, protein and biomass yield recovery in protein enriched extracts from Palmaria palmata (red seaweed) was studied. Protein extraction with alkaline solutions, i.e., NaOH, KOH, Ca(OH)₂ and Na₂CO₃ (at 0.12 M) along with (and without) carbohydrases B, BD and L (containing xylanase, a mixture of xylanase and cellulase, and a combination of cellulase, xylanase and polygalacturonase activities, respectively) was assessed. Principal component analysis was used to assess correlations (if any) between the different alkaline solvents, carbohydrases and changing biomass:solvent on extraction efficiency. The highest protein content (56.64 ± 0.43 % (w/w)) was obtained using KOH assisted extraction with carbohydrase BD. The highest protein and biomass yield recoveries (70.65 ± 1.38 and 21.99 ± 0.43 % (w/w), respectively) were obtained on extraction using NaOH assisted with carbohydrases BD. Electrophoretic, amino acid profile and amino acid score analyses displayed differences depending on the alkaline solvent used for protein extraction. Appropriate selection of the alkaline solvent, carbohydrase and biomass:volume combination during protein extraction can contribute to the production of compositionally enhanced high-quality protein concentrates from P. palmata.

研究了使用不同的碱性溶液、碳水化合物酶以及改变生物质与溶剂的体积比（生物质：溶剂）对棕榈叶（红藻）蛋白质富集提取物中蛋白质含量、蛋白质和生物质产量回收率的影响。评估了使用碱性溶液，即 NaOH、KOH、Ca(OH)2 和 Na2CO3（0.12 M）以及（或不使用）碳水化合物酶 B、BD 和 L（分别含有木聚糖酶、木聚糖酶和纤维素酶的混合物以及纤维素酶、木聚糖酶和聚半乳糖醛酸酶活性的组合）提取蛋白质的情况。采用主成分分析法评估了不同碱性溶剂、碳水化合物酶和生物质：溶剂变化对提取效率的相关性（如有）。使用 KOH 辅助萃取和碳水化合物酶 BD，蛋白质含量最高（56.64 ± 0.43 %（w/w））。在使用 NaOH 和碳水化合物酶 BD 辅助提取时，蛋白质和生物量的回收率最高（分别为 70.65 ± 1.38 % 和 21.99 ± 0.43 %（重量比））。电泳分析、氨基酸谱分析和氨基酸评分分析因提取蛋白质所用的碱性溶剂不同而存在差异。在蛋白质提取过程中适当选择碱性溶剂、碳水化合物酶和生物量：体积组合，有助于从棕榈属植物中生产出成分更优的优质浓缩蛋白质。

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引用次数: 0