Food and Bioproducts Processing最新文献_第5页

CFD-investigation of suspension-based pigs in chocolate processing plants 巧克力加工厂悬浮型猪的cfd调查

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-19 DOI: 10.1016/j.fbp.2025.12.016

Christian Golla , Tobias Hanisch , Christiane Drechsel , Susann Zahn , Marc Mauermann

In chocolate processing plants, cleaning with water-based cleaning agents is not possible since when in contact with water, sugar and milk powder within the chocolate agglomerate which causes blocking of the plants. Common elastomere-based cleaning pigs pose the risk of contaminating through abrasion and have specific design requirements. Research is now focusing on using highly concentrated sugar-in-oil suspensions as flexible cleaning pigs. The rheology of these suspensions is complex, allowing for various compositions. This study presents a simulative pre-design of these pigs using the volume-of-fluid method, targeting challenging components like L-bow pipes and continuous expansions. Simulations systematically varied the rheological properties of the pig-suspension in terms of the Bingham number (

1 0^{- 3}

–

1 0^{2}

) and the Reynolds number (

1 0^{- 4}

–

1 0^{1}

). The massflows investigated were

90, 180, 360 kg/h

. Strong shear-thinning behavior emerged as crucial for creating cohesive pigs with effective cleaning capabilities. No correlation with yield stress was found when the pig was flowable, although values in range

2 – 2000 Pa

were investigated. The tested parameter combinations did not achieve satisfactory contour adaptivity for pigs during continuous expansion. The resulting residual soil layers on the walls and the proportion of soil in the outlet mass flow were up to

50 %

lower than in the reference rinsing process.

在巧克力加工厂，不可能使用水基清洗剂进行清洁，因为它会与巧克力团块中的水、糖和奶粉接触，从而导致工厂堵塞。常见的弹性体清洁清管器存在磨损污染的风险，并且有特定的设计要求。目前的研究重点是使用高度浓缩的油中糖悬浮液作为灵活的清洁猪。这些悬浮液的流变性是复杂的，允许各种成分。本研究采用流体体积法对这些清管器进行了模拟预设计，主要针对l型管和连续膨胀等具有挑战性的部件。模拟系统地改变了猪悬液在宾汉姆数（10−3-102）和雷诺数（10−4-101）方面的流变特性。所研究的质量流量分别为90,180,360kg/h。强大的剪切减薄行为对于创造具有有效清洁能力的粘性猪至关重要。尽管在2-2000Pa范围内进行了研究，但在猪可流动时，没有发现屈服应力与屈服应力的相关性。试验参数组合对猪在连续膨胀过程中的轮廓自适应效果不理想。所得的壁面残留土层和出口质量流中土壤的比例比参考冲洗过程降低了50%。

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引用次数: 0

Establishment and parameter calibration of hazelnut crushing modeling based on DEM 基于DEM的榛子破碎模型的建立及参数标定

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-16 DOI: 10.1016/j.fbp.2025.12.013

Chun Wang , Hailin Kui , Jindai Liu , Zizheng Hu , Xiangyu Liu

In the hazelnut shelling process, issues such as low shelling efficiency and high kernel breakage rate occur due to inappropriate mechanical structure design, working parameter settings, and difficulties in observing the dynamic response characteristics of hazelnuts. Discrete Element Method (DEM) simulation can effectively address these challenges, with simulation model accuracy being crucial. This study established a hollow hazelnut model based on the virtual-physical surface conversion method, Bonding V2 and Meta-Particle model. Contact and bonding parameters of hazelnuts were calibrated through repose tests and uniaxial compression tests using the steepest climbing test and Box-Behnken test. Results showed that when restitution coefficient between hazelnuts was 0.249, static friction coefficient was 0.346, and rolling friction coefficient was 0.018, the relative error of repose angle was only 2.52 %. When normal stiffness per unit area was 4.013 × 10¹² N/m³, shear stiffness per unit area was 4.144 × 10¹² N/m³, normal strength was 2.402 × 10⁹ Pa, shear strength was 4.353 × 10⁸ Pa, and bonded disk scale was 0.869, the relative error of fracture force was 1.48 % and stiffness relative error was 1.27 %. The established model and calibrated parameters accurately reflected the contact and mechanical properties of hazelnuts, providing theoretical support for structural optimization of hazelnut shelling machinery.

在榛子脱壳过程中，由于机械结构设计不合理、工作参数设置不合理、难以观察到榛子的动态响应特性等原因，存在脱壳效率低、果仁破碎率高等问题。离散元法（DEM）仿真可以有效地解决这些挑战，仿真模型的准确性至关重要。本研究基于虚拟-物理表面转换方法、Bonding V2和Meta-Particle模型建立了空心榛子模型。采用最陡爬坡试验和Box-Behnken试验，通过静息试验和单轴压缩试验标定榛子的接触参数和粘结参数。结果表明，当榛子间恢复系数为0.249，静摩擦系数为0.346，滚动摩擦系数为0.018时，休止角的相对误差仅为2.52 %。当正常的单位面积上的刚度是4.013 × 1012 N / m3,单位面积上的剪切刚度是4.144 × 1012 N / m3,正常强度为2.402 × 109 Pa,剪切强度为4.353 × 108 Pa和保税磁盘规模为0.869,断裂力的相对误差为1.48 %和刚度相对误差为1.27 %。所建立的模型和标定参数准确反映了榛子的接触和力学性能，为榛子脱壳机械的结构优化提供了理论支持。

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引用次数: 0

Enzymatic dispersion of pseudomonad biofilms grown at psychrotrophic temperature 在冷养温度下生长的假单胞菌生物膜的酶分散

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-13 DOI: 10.1016/j.fbp.2025.12.015

Srinithi Muthuraman, Jon Palmer, Steve Flint

Pseudomonads are robust biofilm formers in psychrotrophic temperatures, which can cause spoilage in dairy, poultry, and meat processing. This study screened eleven isolates for the biofilm-forming ability using the Congo Red Assay (CRA) and the crystal violet assay. Two isolates, 3SM and 20SM, showed significantly higher EPS production, cellulose synthesis and cell count at 4ºC and were selected for the enzymatic dispersion. Mature biofilms formed on the stainless-steel surface for 72 h at 4°C were treated with laboratory enzymes (Proteinase-K, Cellulase, and DNase I) and commercial enzymes (formulated cleaners, EnduroZyme, DualZyme, and TriZyme). Compared to laboratory enzymes, commercial enzymes were efficient in dispersing the biofilms (EnduroZyme- 62 %, DualZyme- 42 %, and TriZyme-32 % of biofilm removal), which was confirmed by cell counts, crystal violet assay, and microscopic observations. However, none of the treatments resulted in complete biofilm dispersion. These findings highlight the resilience of psychrotrophic pseudomonad biofilms and underscore the need for improved enzymatic strategies tailored for cold-chain environments.

假单胞菌在嗜冷性温度下是健壮的生物膜形成者，可导致乳制品、家禽和肉类加工中的腐败。本研究利用刚果红法（CRA）和结晶紫法对11株菌株进行了生物膜形成能力的筛选。在4ºC条件下，3SM和20SM两株菌株的EPS产量、纤维素合成和细胞计数均显著提高，并被选择用于酶解分散。用实验室酶（蛋白酶- k、纤维素酶和dna酶I）和商业酶（配方清洁剂、EnduroZyme、DualZyme和TriZyme）处理在不锈钢表面形成72 h的成熟生物膜。与实验室酶相比，商业酶对生物膜的分散效率更高（EnduroZyme- 62% %,DualZyme- 42% %，trizyme - 32% %的生物膜去除率），这是通过细胞数量、结晶紫实验和显微镜观察证实的。然而，没有一种处理能使生物膜完全分散。这些发现强调了精神营养假单胞菌生物膜的弹性，并强调了为冷链环境量身定制改进酶策略的必要性。

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引用次数: 0

Innovative cleaning system with movable cavitation nozzle based on the hunting behaviour of the pistol shrimp 创新的清洁系统，可移动的空化喷嘴，基于手枪虾的狩猎行为

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-12 DOI: 10.1016/j.fbp.2025.12.012

R. Murcek , E. Fuchs , J.F. Mauer , M. Finster , M. Mauermann

Submersion-based cleaning methods such as ultrasonic cleaning and pressure flooding are frequently used in industrial cleaning processes. Nevertheless, these approaches exhibit limitations in effectively cleaning difficult-to-reach areas, including holes and grooves, necessitating enhanced methods to improve cleaning performance in such regions. A fluid injector based on the bionic model of the pistol shrimp was developed to generate cavitation jets and it was used for industry-related experimental studies. For this purpose, spot and surface cleaning tests were carried out for flat and complex geometries with a food test model soil. In particular, the influence of the nozzle distance to the surface, the variation of the pulsation parameters and the influence of swelling on cleaning were taken into account. The results were compared with conventional ultrasonic cleaning, whereby it was shown that better cleaning and higher efficiency can be achieved by using immerged cavitation jets. A significant cleaning effect on the surface was measurable up to a nozzle distance of 20 mm. Furthermore, the cleaning time for flat test plates (100 mm×100 mm) could be reduced by more than half compared to conventional ultrasonic cleaning. Nevertheless, it also showed that the new system is only able to clean complex geometries such as drill holes to a limited extent.

超声波清洗和压力驱油等淹没式清洗方法是工业清洗过程中经常使用的方法。然而，这些方法在有效清洁难以到达的区域（包括孔和凹槽）方面表现出局限性，需要改进方法来提高这些区域的清洁性能。基于手枪虾的仿生模型，研制了一种能够产生空化射流的流体喷射器，并将其用于工业相关的实验研究。为此，用食品试验模型土壤对平面和复杂几何形状进行了斑点和表面清洁试验。特别考虑了喷嘴与表面距离的影响、脉动参数的变化以及膨胀对清洗的影响。结果表明，与常规超声清洗相比，浸入式空化射流清洗效果更好，清洗效率更高。可测量到喷嘴距离为20 mm的表面上的显著清洁效果。此外，与传统的超声波清洗相比，平板测试板（100 mm×100 mm）的清洗时间可以减少一半以上。然而，它也表明，新系统只能在有限的范围内清洁复杂的几何形状，如钻孔。

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引用次数: 0

Uncovering the environmental and economic feasibility of an integral tomato-based biorefinery for obtaining pectin and seed oil 揭示一体化番茄生物精炼厂获取果胶和籽油的环境和经济可行性

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-11 DOI: 10.1016/j.fbp.2025.12.011

Adrián Agraso-Otero , Ricardo Rebolledo-Leiva , Sara González-García

The combination of life cycle assessment and techno-economic analysis enables the evaluation of a biorefinery’s viability from both environmental and economic perspectives. This is particularly relevant for valorising food industry by-products within the framework of a circular economy. This study focuses on the integral valorisation of tomato pomace by separating seeds and skins to obtain oil and pectin, respectively. For each valorisation route, two technologies were assessed: cold pressing and supercritical CO₂ extraction for seeds, and acid extraction and ultrasound-assisted extraction for peels. Of these, cold pressing and acid extraction were identified as the best options in terms of environmental performance and yield for seeds and for peels, respectively. The corresponding emissions were 33.6 kg CO₂ eq and 0.08 kg SO₂ eq per kg of oil, and 44.8 kg CO₂ eq and 0.12 kg SO₂ eq per kg of pectin, for global warming and terrestrial acidification respectively. These impacts could be significantly reduced by modifying the heat source, identified as the system’s main hotspot. Economically, the biorefinery with the best environmental performance was not profitable under current conditions. However, sensitivity analysis showed that improving oil yield by a factor of 2.6 through technological adjustments could lead to economic viability. These findings provide valuable insights for stakeholders and policymakers seeking sustainable solutions for food waste management, supporting both environmental responsibility and techno-economic feasibility.

生命周期评估和技术经济分析相结合，可以从环境和经济两方面对生物精炼厂的可行性进行评估。这对于在循环经济框架内对食品工业副产品进行估价尤其重要。本研究的重点是通过分离番茄渣的种子和皮，分别获得油和果胶的整体增值。对于每种增值路线，评估了两种技术：用于种子的冷压和超临界CO 2提取，以及用于果皮的酸提取和超声波辅助提取。其中，从环境性能和种子和果皮的产量来看，冷压和酸提取分别被确定为最佳选择。对于全球变暖和陆地酸化，对应的排放量分别为每公斤石油33.6 kg CO₂eq和0.08 kg SO₂eq，以及每公斤果胶44.8 kg CO₂eq和0.12 kg SO₂eq。这些影响可以通过改变被确定为系统主要热点的热源来显著减少。从经济角度看，环境性能最好的生物精炼厂在目前条件下是不盈利的。然而，敏感性分析表明，通过技术调整将石油产量提高2.6倍可以实现经济可行性。这些发现为寻求食物垃圾管理的可持续解决方案的利益相关者和决策者提供了宝贵的见解，支持环境责任和技术经济可行性。

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引用次数: 0

Solid-liquid phenolic compound transfer and kinetic modeling during cachaça maturation using tropical woods 利用热带木材制备的仙人掌成熟过程中固-液酚类化合物转移及动力学模型

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-11 DOI: 10.1016/j.fbp.2025.12.010

Jackson de Souza Moreira , Ana Beatriz da Costa Pereira Silva , Normando Ribeiro-Filho , Flávio Luiz Honorato da Silva , Líbia de Sousa Conrado

The maturation of spirits involves complex interactions between wood composition, chemical extraction, and sensory development. This study investigated the transfer of phenolic compounds during cachaça ageing with Brazilian tropical woods (amburana, bálsamo, and castanheira), employing kinetic modelling to elucidate diffusion mechanisms, optimise wood utilisation, and enhance antioxidant and sensory properties. Woods were characterised for lignocellulosic composition following TAPPI standards, and cachaça maturation was conducted under first use, second use, and continuous 70-day regimes at 4 g of wooden chips/L. Total phenolic content, antioxidant activity (DPPH, ABTS, FRAP), and colour (CCU) were measured. Phenolic extraction kinetics were fitted to Lagergren, Peleg, intraparticle diffusion, and parabolic models. Amburana promoted rapid phenolic migration and high antioxidant activity, bálsamo yielded higher total phenolics but lower DPPH responses, and castanheira contributed moderate phenolic release and colour development. Continuous maturation enhanced functional and visual properties, while wood re-use reduced efficiency. Kinetic modelling confirmed a two-step extraction and validated the pseudo-first-order Lagergren model. These findings demonstrate the potential of tropical woods as sustainable oak alternatives for premium cachaça production.

烈酒的成熟涉及木材成分、化学提取和感官发育之间复杂的相互作用。本研究研究了巴西热带木材（amburana， bálsamo和castanheira）在cachaa老化过程中酚类化合物的转移，采用动力学模型来阐明扩散机制，优化木材利用，增强抗氧化和感官性能。按照TAPPI标准对木材的木质纤维素成分进行表征，在4 g木屑/L的条件下，在第一次使用、第二次使用和连续70天的条件下进行cacha成熟。测定总酚含量、抗氧化活性（DPPH、ABTS、FRAP）和颜色（CCU）。苯酚萃取动力学符合Lagergren， Peleg，颗粒内扩散和抛物模型。Amburana促进了酚类物质的快速迁移和高抗氧化活性，bálsamo产生了较高的总酚类物质，但较低的DPPH响应，castanheira促进了适度的酚类物质释放和显色。连续熟化增强了木材的功能和视觉特性，而木材的重复使用降低了效率。动力学模型证实了两步提取，并验证了伪一阶Lagergren模型。这些发现证明了热带木材作为优质cachaa生产的可持续橡木替代品的潜力。

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引用次数: 0

Comparative study of hydrodistillation and microwave-assisted technique for dill seed (Anethum graveolens) essential oil extraction: Process kinetics, yield, composition, and sustainability 加氢蒸馏和微波辅助提取莳萝籽精油的比较研究：过程动力学、产率、组成和可持续性

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-10 DOI: 10.1016/j.fbp.2025.12.009

Danica Božović , Nevena Gladikostić , Bojana Ikonić , Nemanja Teslić , Aleksandra Sknepnek , Milica Mirković , Sanja Vojvodić , Zoran Zeković , Predrag Putnik , Danijela Bursać Kovačević , Branimir Pavlić

This study compares hydrodistillation (HD) and microwave-assisted hydrodistillation (MWHD) for extracting essential oil (EO) from dill seeds (Anethum graveolens), targeting applications in food and pharmaceutical industries. Both methods were conducted at varying power levels, analyzing EO yield, antioxidant and antimicrobial activity, and environmental impact. Microwave-assisted hydrodistillation at 600 W achieved the highest EO yield (3.23 %) with faster extraction kinetics, whereas hydrodistillation exhibited lower CO₂ emissions, emphasizing its environmental sustainability. The EO from both methods was rich in terpenes, with limonene and carvone as primary components. MWHD at 800 W exhibited the strongest antioxidant activity in ABTS and DPPH assays, and both techniques showed effective antimicrobial properties, especially against Gram-negative bacteria and yeast. Kinetic modeling revealed that models including diffusion and washing phases best fit the extraction data (R² > 0.99). These findings support MWHD as a viable alternative for EO extraction, with optimization potential for industrial scale-up.

本研究比较了加氢蒸馏法（HD）和微波辅助加氢蒸馏法（MWHD）从莳萝种子（Anethum graveolens）中提取精油（EO）的方法，以期在食品和制药工业中得到应用。两种方法都在不同的功率水平下进行，分析了EO的产率、抗氧化和抗菌活性以及对环境的影响。微波辅助加氢蒸馏法在600 W下获得了最高的EO收率（3.23 %），萃取动力学更快，而加氢蒸馏法的CO₂排放量更低，强调了其环境可持续性。两种方法得到的精油均含有丰富的萜烯，主要成分为柠檬烯和香芹酮。800 W的MWHD在ABTS和DPPH实验中表现出最强的抗氧化活性，两种技术都表现出有效的抗菌性能，特别是对革兰氏阴性菌和酵母。动力学模型表明，包括扩散相和洗涤相的模型最适合提取数据（R²> 0.99）。这些发现支持MWHD作为EO提取的可行替代方案，具有工业规模优化潜力。

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引用次数: 0

Improving betacyanins stability by sustainable application of xanthan gum, CMC and citric acid in fermented red dragon fruit drink: Long-term storage, bio-accessibility and antioxidant activity 黄原胶、CMC和柠檬酸持续应用于红龙果发酵饮料中，提高红龙果苷的稳定性：长期储存、生物可及性和抗氧化活性

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-09 DOI: 10.1016/j.fbp.2025.12.008

Teck Wei Lim , Renee Lay Hong Lim , Liew Phing Pui , Chin Ping Tan , Chun Wai Ho

Red dragon fruit (Hylocereus polyrhizus) with high betacyanins content is a potential commercial fruit that can be developed into a functional drink. The development of a functional product not only requires innovation but also needs to consider the sustainability of the raw materials and techniques employed. The present study aimed to employ mild and sustainable approaches via the incorporation of xanthan gum (XG) and carboxymethyl cellulose (CMC) hydrocolloid mixture solution and citric acid (CA) at optimum concentrations to modify the matrix of fermented red dragon fruit drink (FRDFD) to produce Improved-FRDFD-dH₂O with improved long-term storage stability, bio-accessibility and antioxidant activity of the betacyanins. Results revealed that 0.4 % XG and 0.5 % CMC hydrocolloid mixture solution was able to synergistically cooperate with 0.2 % CA for providing the best food matrix environment for the maximum betacyanins stability (<10 % degradation) throughout the 8-week storage period at 25°C. The simulated digestion study showed that the incorporation of 0.4 % XG and 0.5 % CMC hydrocolloid mixture solution and 0.2 % CA successfully improved the bio-accessibility (>80 % retained) and preserved the high antioxidant activities of betanin throughout the digestive process (up to 94.18 % free radicals inhibited).

红火龙果（Hylocereus polyrhizus）具有较高的甜菜菁苷含量，是一种具有开发功能饮料潜力的商品水果。功能性产品的开发不仅需要创新，还需要考虑所采用的原材料和技术的可持续性。本研究旨在采用温和和可持续的方法，将黄原胶（XG）和羧甲基纤维素（CMC）水胶体混合溶液和柠檬酸（CA）以最佳浓度对发酵红龙果饮料（FRDFD）的基质进行修饰，生产出具有较好长期储存稳定性、生物可及性和抗氧化活性的改良型红龙果饮料（FRDFD）。结果表明，0.4 % XG和0.5 % CMC的水胶体混合溶液能与0.2 % CA协同作用，为甜菜菁苷在25°C条件下8周的最大稳定性（降解率为10 %）提供了最佳的食品基质环境。模拟消化研究表明，加入0.4 % XG和0.5 % CMC的水胶体混合溶液和0.2 % CA，成功地提高了甜菜素的生物可及性（保留80 %），并在整个消化过程中保持了甜菜素的高抗氧化活性（高达94.18 %的自由基抑制）。

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引用次数: 0

Corrigendum to “Incorporation of zein nanofibers produced by needle-less electrospinning within the casted gelatin film for improvement of its physical properties” [Food Bioprod. Process. 122 (2020) 193–204] “在铸造明胶薄膜中加入无针静电纺丝生产的玉米蛋白纳米纤维以改善其物理性能”的勘误表[食品生物制品]。Process. 122 (2020) 193-204]

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-09 DOI: 10.1016/j.fbp.2025.12.005

Mehri Karim, Milad Fathi, Sabihe Soleimanian-Zad

引用次数: 0

Survival of Staphylococcus aureus, Listeria monocytogenes, and Salmonella spp. in traditional Tomas cheese during the ripening at different temperatures 传统托马斯奶酪中金黄色葡萄球菌、单核增生李斯特菌和沙门氏菌在不同温度成熟过程中的存活

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing

Pub Date : 2025-12-06 DOI: 10.1016/j.fbp.2025.12.006

Muhsin Mutlu , Gokhan Kursad Incili , Mehmet Calicioglu

This study aimed to investigate the viability of Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp. in Tomas cheese produced by traditional methods and ripened at 4 °C and 10 °C. Fresh Tomas cheese samples were inoculated with a cocktail of Staphylococcus aureus, Listeria monocytogenes, and Salmonella spp. (approximately 4.0–5.0 log₁₀ CFU/g) and then pressed into plastic jars and stored at 4 °C and 10 °C. Chemical and microbiological analyses of the cheese were performed on days 0, 7, 15, 30, 45, 60, 90, and 120. At the end of the ripening period, the mean reductions in pathogen counts in the groups stored at 4 °C and 10 °C were 4.54 and 3.15 log₁₀ CFU/g for Salmonella spp. (p < 0.001), 1.59 and 1.75 log₁₀ CFU/g for Staphylococcus aureus, and 2.92 and 3.06 log₁₀ CFU/g for Listeria monocytogenes, respectively. The total viable counts (TVC), psychrotrophic bacteria, Lactobacillus spp., Lactococcus spp., Enterococcus spp., proteolytic bacteria, lipolytic bacteria, mold and yeast counts remained within the 4–7 log₁₀ CFU/g range with only slight fluctuations during the ripening period, and no significant differences were detected between the groups at the two storage temperatures. As a result, although Staphylococcus aureus, Salmonella spp., and Listeria monocytogenes counts decreased at two both ripening temperatures (4 °C and 10 °C) by the end of the ripening period, the data suggest that these pathogenic microorganisms may still pose a potential food safety risk to public health in Tomas cheese.

本研究旨在研究4℃和10℃成熟的thomas奶酪中单核细胞增生李斯特菌、金黄色葡萄球菌和沙门氏菌的生存能力。用金黄色葡萄球菌、单核增生李斯特菌和沙门氏菌混合菌（约4.0-5.0 log10 CFU/g）接种新鲜Tomas奶酪样品，压入塑料罐中，分别于4℃和10℃保存。在第0、7、15、30、45、60、90和120天对奶酪进行化学和微生物分析。成熟期结束时，4°C和10°C贮藏组的病原菌计数平均减少量分别为沙门氏菌4.54和3.15 log10 CFU/g (p <； 0.001)，金黄色葡萄球菌1.59和1.75 log10 CFU/g，单核增生李斯特菌2.92和3.06 log10 CFU/g。总活菌数（TVC）、嗜冷菌数、乳杆菌数、乳球菌数、肠球菌数、水解蛋白菌数、溶脂菌数、霉菌数和酵母菌数均保持在4-7 log10 CFU/g范围内，在成熟期只有轻微波动，两种贮藏温度下各组间无显著差异。因此，尽管在成熟期结束时，在4°C和10°C两个成熟温度下，金黄色葡萄球菌、沙门氏菌和单核细胞增生李斯特菌的数量都有所减少，但数据表明，这些致病微生物仍可能对Tomas奶酪的公众健康构成潜在的食品安全风险。

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引用次数: 0