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Non-destructive acoustic-visual detection of oyster fatness 牡蛎脂肪无损声视觉检测
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-05 DOI: 10.1016/j.fbp.2025.12.007
Bin Chen , Laslo Tarjan , Gongming Wang , Yimin Ma , Feng Liu , Jiahao Yu , Huanhuan Feng , Xiaoshuan Zhang
Traditional oyster grading methods based solely on shell weight often fail to accurately reflect internal fattening levels, leading to inconsistent quality and reduced consumer trust. This study introduces a non-destructive detection method for evaluating the fattening degree of closed-shell oysters by integrating acoustic vibration analysis with visual morphological information. A multimodal sensing system was developed, combining mechanical tapping, acoustic signal acquisition, and dual-view imaging to simultaneously capture internal vibration responses and external shell features. Time-domain, frequency-domain, and time–frequency-domain acoustic features were extracted alongside geometric features from top and side views. These were standardized and fused using Z-score normalization and Principal Component Analysis (PCA) to form comprehensive feature vectors. A deep learning model based on CNN-attention-BiLSTM architecture was employed to classify oysters into four fattening levels. The proposed method achieved high classification accuracy and demonstrated strong robustness, outperforming traditional single-modality approaches. This work provides an effective and scalable solution for intelligent, non-invasive oyster quality grading and offers new insights into multimodal assessment of shellfish products.
传统的牡蛎分级方法仅基于壳重,往往不能准确反映内部脂肪水平,导致质量不一致,降低了消费者的信任度。介绍了一种将声振动分析与视觉形态信息相结合的闭壳牡蛎育肥程度无损检测方法。开发了一种多模态传感系统,结合机械敲击、声信号采集和双视图成像,同时捕获内部振动响应和外壳特征。时域、频域和时频域声学特征与顶部和侧面的几何特征一起被提取。使用Z-score归一化和主成分分析(PCA)对这些进行标准化和融合,形成综合特征向量。采用基于CNN-attention-BiLSTM架构的深度学习模型,将牡蛎分为4个肥胖水平。该方法具有较高的分类精度和较强的鲁棒性,优于传统的单模态方法。本研究为智能、无创的牡蛎质量分级提供了有效、可扩展的解决方案,并为贝类产品的多模式评估提供了新的见解。
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引用次数: 0
The effects of biofilm formation of Hafnia paralvei mediated by quorum sensing on the meat quality and spoilage of crucian carp (Carassius auratus) 群体感应介导的黑花鱼生物膜形成对鲫鱼肉品质和腐败的影响
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-04 DOI: 10.1016/j.fbp.2025.12.004
Wenfang Niu , Wenqing Yang , Yuyang Duan , Chao Gao , Zhe Chen , Yi Li
Hafnia paralvei, as an important foodborne pathogen with a strong ability to form biofilms, will cause food to spoil and deteriorate, thereby seriously threatening food safety. However, the mechanism of the quorum sensing (QS) system in the biofilm formation and spoilage process of H. paralvei has not been fully characterized. Here, we explored the positive regulatory effects of two types of QS system on H. paralvei Z11 biofilm formation, which further investigated their promoting effects on the spoilage of crucian carp. The results showed that overexpression of expI significantly increased the production of N-acyl-homoserine lactones (AHLs), and overexpression of luxS significantly increased the yield of autoinducer-2 (AI-2). Additionally, AHLs and AI-2-mediated QS promoted the synthesis of cyclic diguanylate monophosphate (c-di-GMP), thereby increasing the level of biofilm formation. The effects of biofilm formation mediated by AHLs and AI-2 on the meat quality and spoilage of crucian carp were determined, which found that the elevated biofilm formation significantly lowered the meat quality and promoted the spoilage potential of H. paralvei on crucian carp, simultaneously affecting the structure of meat microbiota and increasing the abundance of spoilage-associated bacteria. This research provides a theoretical foundation for further understanding the potential role of bacterial QS system mediated biofilm formation in food spoilage.
弓形虫是一种重要的食源性病原体,具有很强的生物膜形成能力,会导致食品变质变质,严重威胁食品安全。然而,群体感应(quorum sensing, QS)系统在疟原虫生物膜形成和腐败过程中的作用机制尚未得到充分表征。本研究探讨了两种QS系统对副毒弧菌Z11生物膜形成的正向调节作用,进一步探讨了它们对鲫鱼腐败的促进作用。结果表明,过表达expI可显著提高n -酰基-高丝氨酸内酯(AHLs)的产量,过表达luxS可显著提高自诱导因子2 (AI-2)的产量。此外,AHLs和ai -2介导的QS促进了环二胍酯单磷酸(c-di-GMP)的合成,从而提高了生物膜的形成水平。测定了AHLs和AI-2介导的生物膜形成对鲫鱼肉品质和腐败的影响,发现生物膜形成的升高显著降低了鲫鱼肉品质,提高了副弧菌对鲫鱼的腐败潜力,同时影响了肉质微生物群的结构,增加了腐败相关细菌的丰度。本研究为进一步认识细菌QS系统介导的生物膜形成在食品腐败中的潜在作用提供了理论基础。
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引用次数: 0
First insights into changing cleaning mechanisms based on experimental and numerical investigations 基于实验和数值研究的首次见解改变了清洗机制
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-03 DOI: 10.1016/j.fbp.2025.12.001
H. Köhler , C. Golla , C. Drechsel , F. Rüdiger
Cleaning is a crucial process step within food production, as it has a major impact on food safety and involves considerable resource consumption. There are various approaches to optimize cleaning processes, e.g., i) cleaning experiments on laboratory scale, ii) specific soil characterization methods on laboratory scale and iii) cleaning simulations. This paper shows for the first time how all three methods are combined to understand and model complex behavior during cleaning. To this end, removal of dried ketchup soil layers was studied using aqueous sodium hydroxide at various concentrations (0 – 2 wt%) and temperatures (20 – 55 °C) as cleaning fluid. In cleaning experiments, significantly shorter cleaning times are measured when cleaning is performed with hot 2 wt% sodium hydroxide solution (69 s) instead of pure water at room temperature (167 s). Counterintuitively, micromanipulation and rheological measurements of the soil layer show a solidification when in contact with sodium hydroxide as opposed to water. It is found that this is due to a change of the cleaning mechanism from pure adhesive detachment to combined cohesive separation of an interlayer, followed by adhesive detachment of the remaining soil. A recently developed cleaning model is extended to capture the ongoing phenomena. The model is able to predict the cleaning time correctly with errors smaller than 10 %.
清洁是食品生产过程中的一个关键步骤,因为它对食品安全有重大影响,并涉及相当大的资源消耗。有各种方法可以优化清洗过程,例如,i)实验室规模的清洗实验,ii)实验室规模的特定土壤表征方法和iii)清洗模拟。本文首次展示了如何将这三种方法结合起来理解和模拟清洁过程中的复杂行为。为此,采用不同浓度(0 - 2 wt%)和温度(20 - 55℃)的氢氧化钠水溶液作为清洗液,研究了干燥番茄酱土层的去除。在清洗实验中,用2 wt%的氢氧化钠热溶液(69 秒)代替室温下的纯水(167 秒)进行清洗时,清洗时间明显缩短。与直觉相反,对土层的微观操作和流变测量表明,当与氢氧化钠接触时,而不是与水接触时,土壤会凝固。研究发现,这是由于清洗机制发生了变化,从单纯的粘着分离到夹层的联合粘着分离,然后是剩余土壤的粘着分离。最近开发的清洁模型被扩展到捕捉正在进行的现象。该模型能够准确预测清洗时间,误差小于10 %。
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引用次数: 0
Co-pH altered modified goose liver protein with enhanced interfacial stabilization and its emulsion filling upon the goose MP gelation facilitation 共ph改变了改性鹅肝蛋白的界面稳定性,增强了其乳化填充对鹅肝蛋白凝胶化的促进作用
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-03 DOI: 10.1016/j.fbp.2025.12.003
Shiyue Tan , Limai Tang , Yangying Sun , Xiaoqun Zeng , Hongbing Yan , Yangyang Hu , Daodong Pan
This study aimed to explore the stability and functionality of emulsions stabilized by modified goose liver protein (MGLP) at different pH values (7.0–11.0) from an interfacial perspective. Results of interfacial pressure and shear elasticity showed that there was a pH dependent change of MGLP adsorption and associative interaction, with the values both reached highest (11.42 ± 0.22 mN/m and 16.30 ± 0.35 mN/m) at pH 8.0. Droplet characteristics revealed that MGLP at pH 8.0 with the mostly cross-linked interface layer displayed the lowest D [4,3] (6.61 ± 0.53 μm), flocculation (5.07 ± 0.09 %), creaming (16.20 ± 0.38 %) and turbiscan stability index (8.27 ± 0.16); and also the high stability of the emulsion over a range of environmental conditions: temperature < 60 °C; pH 7–10 and NaCl < 50 mmol/L. Low field-nuclear magnetic resonance and dynamic rheological analysis indicated that the intermolecular association and goose myofibrillar protein (MP) gelation were mostly strengthened at the MGLP emulsion8.0 to MP ratio of 3 %, giving the largest water holding capacity and storage modulus. To conclude, MGLP emulsion exhibited good interfacial stabilization through facile pH alteration of the protein, which showed the capacity in fortifying goose MP gelation through active filling effect.
本研究旨在从界面角度探讨改性鹅肝蛋白(MGLP)在不同pH值(7.0 ~ 11.0)下稳定乳剂的稳定性和功能。界面压力和剪切弹性结果表明,MGLP吸附和结合相互作用的pH值随pH值的变化而变化,pH值为8.0时达到最大值(11.42 ± 0.22 mN/m和16.30 ± 0.35 mN/m)。滴特征表明MGLP pH值8.0主要交联接口层显示最低的D[4 3](6.61 ± 0.53μm),絮凝(5.07 ±0.09  %),乳化(16.20 ±0.38  %)和turbiscan稳定指数(8.27 ±0.16 );以及乳液在一系列环境条件下的高稳定性:温度<; 60°C;pH 7-10和NaCl <; 50 mmol/L。低场核磁共振和动态流变学分析表明,当MGLP乳剂的分子量比为8.0 %时,分子间的结合和鹅肌纤维蛋白(MP)的凝胶化得到加强,具有最大的持水能力和储存模量。综上所述,MGLP乳状液通过改变蛋白的pH值表现出良好的界面稳定性,显示出通过活性填充效应强化鹅MP凝胶化的能力。
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引用次数: 0
Liquid jet impingement on solid walls: A review 液体射流对固体壁面的冲击研究进展
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-03 DOI: 10.1016/j.fbp.2025.12.002
Haichao Sun, Xikun Wang, Yalin Li, Xianming Tao
Liquid jet impingement on solid surfaces holds significant value in industrial cleaning applications, particularly within the food and bioproducts sector. This study systematically analyzes the physical mechanisms governing the jet impingement process, focusing on the liquid film formation dynamics and their correlation with cleaning performance. Key parameters controlling liquid film morphology are identified and classified into four primary categories: namely, gravitational effects, fluid transport properties (flow rate, viscosity, and density), interface effects (contact angle), and nozzle geometrical characteristics (shape and diameter). By synthesizing existing research findings, a unified framework is proposed for prediction models and advanced measurement techniques related to liquid film radial expansion and thickness distribution. Regarding jet impingement dynamics, models for predicting wall shear stress are analyzed, highlighting the specific roles of jet Reynolds number and nozzle-to-plate distance. A comparative analysis of experimental studies on jet impact force reveals a deficiency of systematic theoretical models. For non-planar surface configurations, this review consolidates experimental and computational findings regarding concave surface impingement, illustrating the pronounced influence of surface curvature radius on liquid film distribution. A notable research gap is identified for jet impingement on spherical concave surfaces. Finally, multiple experimental datasets for jet cleaning of soil layers are compiled, and the primary evaluation methodologies are compared in terms of their respective strengths and limitations. It is emphasized that a comprehensive framework for assessing jet cleaning performance should incorporate all pertinent physical parameters and operational conditions to achieve broader applicability.
液体射流对固体表面的冲击在工业清洁应用中具有重要的价值,特别是在食品和生物制品领域。本研究系统分析了射流冲击过程的物理机制,重点研究了液膜形成动力学及其与清洗性能的关系。确定了控制液膜形态的关键参数,并将其分为四大类:即引力效应、流体输运特性(流量、粘度和密度)、界面效应(接触角)和喷嘴几何特性(形状和直径)。在综合已有研究成果的基础上,提出了液膜径向膨胀和厚度分布预测模型和先进测量技术的统一框架。在射流冲击动力学方面,分析了预测壁面剪应力的模型,强调了射流雷诺数和喷嘴到板的距离的具体作用。通过对射流冲击力实验研究的对比分析,发现缺乏系统的理论模型。对于非平面表面构型,本文综合了关于凹表面撞击的实验和计算结果,说明了表面曲率半径对液膜分布的显著影响。射流对球面凹面的冲击是一个显著的研究空白。最后,编制了多个土层喷淋净化实验数据集,比较了几种主要评价方法的优缺点。强调评价喷气机清洁性能的综合框架应包括所有有关的物理参数和操作条件,以实现更广泛的适用性。
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引用次数: 0
Efficient fermentation of di-rhamnolipids-rich products by P. aeruginosa using ethanol as solely carbon source 铜绿假单胞菌以乙醇为唯一碳源高效发酵富含大鼠李糖脂的产物
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-02 DOI: 10.1016/j.fbp.2025.11.027
Xingyu Liu , Li Luo , Mengqian Zhao , Deyu Zhang , Bo Chen , Jun Liu , Xuwei Long
High raw material costs critically limit rhamnolipids' industrial competitiveness against chemical synthetic surfactants. To address this, we developed a new fermentation process using low-cost ethanol (including industrial waste) as the sole carbon source by Pseudomonas aeruginosa PAO1. Through fed-batch optimization, a record rhamnolipid titer of 20.74 g/L was achieved—fivefold higher than batch fermentation. Further implementing sequential fed-batch fermentation over 21 days yielded an unprecedented cumulative production of 117.78 g/L, corresponding to 0.234 g/L/h average productivity (117 % improvement over optimized fed-batch fermentation). Crucially, ethanol-derived products contained 85.6 % di-rhamnolipids—significantly higher than conventional systems—while exhibiting performance parity with soybean oil-sourced counterparts. This work establishes a scalable, cost-efficient platform for high-value biosurfactant production, reducing dependency on expensive substrates while enabling industrial waste valorization.
高昂的原材料成本严重限制了鼠李糖脂相对于化学合成表面活性剂的工业竞争力。为了解决这一问题,我们开发了一种新的发酵工艺,利用低成本乙醇(包括工业废料)作为铜绿假单胞菌PAO1的唯一碳源。通过分批发酵优化,鼠李糖脂滴度达到创纪录的20.74 g/L,比分批发酵提高了5倍。进一步实施连续分批发酵21天,获得了前所未有的累积产量117.78 g/L,相当于0.234 g/L/h的平均产量(比优化后的分批发酵提高117 %)。至关重要的是,乙醇衍生产品含有85.6% %的鼠李糖脂,显著高于传统系统,同时表现出与豆油衍生产品相同的性能。这项工作为高价值生物表面活性剂的生产建立了一个可扩展的、具有成本效益的平台,减少了对昂贵底物的依赖,同时实现了工业废物的增值。
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引用次数: 0
Understanding cleaning of molasses foulants: An industrial case study 了解糖蜜污垢的清洗:一个工业案例研究
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-02 DOI: 10.1016/j.fbp.2025.11.028
Natpawi Intha, Phanida Saikhwan
The cleaning of molasses foulants formed in the distillation column of an ethanol production plant was investigated to elucidate the cleaning mechanisms and identify more efficient operating conditions. The plant currently uses sodium hydroxide (NaOH) solutions (15–20 wt%) due to safety and corrosion concerns associated with strong acids. Swelling/dissolution studies of the foulants in NaOH were conducted by monitoring foulant mass change over time. Changes in foulant composition and morphology were then analysed using XRF, FT-IR, and SEM-EDS. Optimal NaOH concentrations were found to be 10 wt% at room temperature and 8 wt% at 80°C—both lower than the concentrations currently used in the investigated plant. Although higher temperature did not increase the overall extent of removal, it substantially reduced deposit mechanical strength. Cross-sectional SEM-EDS showed deeper NaOH penetration and removal of subsurface calcium sulphate (CaSO₄), explaining the weakened structure. Increasing mass transfer at room temperature by agitating the NaOH solution similarly enhanced the penetration and reduced deposit strength. While NaOH alone did not achieve complete removal, it effectively weakened the foulants, which may shorten subsequent mechanical cleaning time. Swelling and dissolution studies using citric acid (optimal concentration 3 wt%) were also performed to evaluate the potential of a weak acid cleaner. Citric was selected because its chelating ability. Although calcium, the major component of molasses foulants was removed by citric acid, lower cleaning efficiency (lower dissolution rate and extent of removal) than NaOH was observed. Overall, this study provides a mechanistic understanding of alkali cleaning of molasses foulants and demonstrates improved cleaning efficiency—greater removal, shorter times, and weaker residual deposits—using 10 wt% NaOH under flow conditions. The findings offer guidance for optimising alkali-based cleaning strategies for molasses foulants and other complex organic–mineral deposits.
对乙醇生产装置蒸馏塔中形成的糖蜜杂质的清洗进行了研究,阐明了清洗机理,并确定了更有效的操作条件。由于与强酸相关的安全性和腐蚀问题,该工厂目前使用氢氧化钠(NaOH)溶液(15-20 wt%)。通过监测污染物质量随时间的变化,进行了污染物在NaOH中的溶胀/溶解研究。然后使用XRF, FT-IR和SEM-EDS分析污染物组成和形态的变化。在室温下,NaOH的最佳浓度为10 wt%,在80°c时为8 wt%,这两个浓度都低于目前在所研究的植物中使用的浓度。虽然较高的温度并没有增加整体的去除程度,但却大大降低了镀层的机械强度。横截面SEM-EDS显示NaOH渗透更深,表面下硫酸钙(CaSO₄)被去除,解释了结构减弱的原因。在室温下通过搅拌NaOH溶液来增加传质,同样增强了渗透并降低了镀层强度。虽然NaOH本身不能完全去除,但它可以有效地削弱污垢,从而缩短后续的机械清洗时间。使用柠檬酸(最佳浓度3 wt%)进行溶胀和溶解研究,以评估弱酸清洁剂的潜力。选择柠檬是因为它的螯合能力。柠檬酸虽然可以去除糖蜜杂质的主要成分钙,但其去除效率(溶解速度和去除程度)低于氢氧化钠。总的来说,本研究提供了对糖蜜污垢的碱清洗机理的理解,并证明了在流动条件下使用10 wt%的NaOH可以提高清洗效率,去除效果更好,时间更短,残留沉积物更弱。该研究结果为优化碱基清洗糖蜜污垢和其他复杂有机矿床的策略提供了指导。
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引用次数: 0
Experimental and simulation study of heat and mass transfer during drying-shelling of Camellia oleifera fruit with changing diffusion pathways 不同扩散途径下油茶果实干燥脱壳传热传质的实验与模拟研究
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.fbp.2025.11.022
Pei Yang, Hanyu Fu, Penggeng Sun, Yanlong Bu, Jun Zhang, Deyong Yang
This study investigates the heat and mass transfer characteristics of the C. oleifera shell and the shelling mechanism during the drying-shelling process from the perspective of the biological characteristics of capsular fruits. Hot air drying experiments were conducted to explore the effect of the capsular structure on drying characteristics. A 3D drying model, based on the differing moisture diffusion pathways of the shell in the pre- and post-dehiscence stages, was developed to reveal the evolution of moisture and temperature distribution. The model was then validated against experimental data from physical dissections and thermal imaging. The results showed that prior to fruit dehiscence, moisture was lost primarily through the outer surface, and the process included a distinct accelerating drying period. After dehiscence, the moisture diffusion pathway gradually stabilized, and the process entered a falling-rate drying period. This difference in pathway influenced the shell's moisture diffusion, as the effective moisture diffusivity for unidirectional diffusion was greater than that for bidirectional diffusion. Throughout the drying-shelling process, the actual internal temperature of the shell remained below the drying temperature, and the non-uniformity of the surface temperature gradually decreased after the preheating stage. The simulation results were in good agreement with the experimental data. By predicting the hygrothermal distribution in the shells of capsular fruits, this study provides a better understanding of the drying-shelling mechanism under different conditions and offers a robust method for rationally selecting processing parameters.
本研究从荚果生物学特性的角度出发,研究了油橄榄果壳在干燥-脱壳过程中的传热传质特性及脱壳机理。通过热风干燥实验,探讨了胶囊结构对干燥特性的影响。基于壳在破裂前和破裂后不同的水分扩散路径,建立了三维干燥模型,揭示了壳的水分和温度分布的演变。然后根据物理解剖和热成像的实验数据验证该模型。结果表明,在果实开裂前,水分主要通过外表面流失,并有明显的加速干燥期。开裂后,水分扩散路径逐渐稳定,进入降速干燥期。这种路径的差异影响了壳的水分扩散,单向扩散的有效水分扩散率大于双向扩散。在整个干燥-脱壳过程中,壳的实际内部温度保持在干燥温度以下,表面温度的不均匀性在预热阶段后逐渐降低。仿真结果与实验数据吻合较好。通过对荚果壳内湿热分布的预测,可以更好地了解不同条件下的干燥脱壳机理,并为合理选择加工参数提供可靠的方法。
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引用次数: 0
Bioactive compounds in Argania spinosa oil: A promising approach for engineered skin and wound repair 棘豆油中的生物活性化合物:工程皮肤和伤口修复的一种有前途的方法
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.fbp.2025.11.014
Ahmed Boukeloua , Hamdi Bendif , Badis Aouzal , Nabila Souilah , Mustafa Abdullah Yilmaz , Mehmet Boga , Abdelghafar M. Abu-Elsaoud , Walid Elfalleh , Hamdi Temel , Fehmi Boufahja , Stefania Garzoli
Argania spinosa oil, extracted from the kernels of the Argan tree, has been traditionally valued for its therapeutic and healing properties. However, its potential as a bioengineered solution for wound healing and skin regeneration has not been comprehensively explored. This study bridges that gap by evaluating the chemical composition, biological activity, and safety profile of Argania spinosa oil for potential biomedical applications. The oil was extracted using ethanol and hexane and characterized by its physicochemical properties. Gas chromatography and spectrophotometric analyses revealed a high content of unsaturated fatty acids, primarily oleic acid (44.40 %) and linoleic acid (36.69 %), alongside significant levels of phytosterols (134.05 mg/100 g) and α-tocopherol (7.44 mg/100 g). The ethanolic extract showed strong antioxidant potential, with total phenolic and flavonoid contents of 67.47 μg/mg and 31.90 μg/mg, respectively. Biological assessments in rabbits demonstrated that wounds treated with the oil achieved 98.7 % closure by day 12, compared to 93.77 % in controls, confirming its efficacy in accelerating tissue repair. The healing effect is attributed to its bioactive compounds that enhance vascularization and tissue regeneration. Safety evaluations, including acute toxicity, ocular irritation, and dermal irritation tests, confirmed that the oil is non-toxic and non-irritating, with no mortality observed up to doses of 70 mL/kg (oral) and 6 mL/kg (intraperitoneal) in mice. Additionally, no adverse reactions were observed on rabbit skin or rectal mucosa. Key phenolic constituents, such as quinic acid (4.09 μg/g), coumarin (1.60 μg/g), and hesperidin (0.50 μg/g), were identified, contributing to its antioxidant and anti-inflammatory properties. The high polyunsaturated-to-saturated fatty acid ratio (3.10) further underscores its therapeutic and nutritional significance. This study establishes Argania spinosa oil as a promising, natural, and safe candidate for bioengineered skin applications. By addressing the gap in empirical data on its wound-healing mechanisms and safety, the research supports its use in developing sustainable, temporary, and permanent engineered skin substitutes for regenerative medicine. Future studies should focus on elucidating its molecular pathways, optimizing extraction techniques, and assessing long-term clinical safety.
Argania spinosa油,从Argan树的内核中提取,传统上因其治疗和愈合特性而受到重视。然而,它作为伤口愈合和皮肤再生的生物工程解决方案的潜力尚未得到全面的探索。本研究通过评估棘豆油的化学成分、生物活性和潜在生物医学应用的安全性来弥补这一差距。以乙醇和己烷为萃取剂,对其理化性质进行了表征。气相色谱和分光光度分析显示,不饱和脂肪酸含量高,主要是油酸(44.40 %)和亚油酸(36.69 %),同时植物甾醇(134.05 mg/100 g)和α-生育酚(7.44 mg/100 g)含量显著。乙醇提取物具有较强的抗氧化潜力,总酚含量为67.47 μg/mg,总黄酮含量为31.90 μg/mg。兔的生物学评估表明,与对照组的93.77 %相比,使用油治疗的伤口在第12天达到98.7 %的愈合,证实了其加速组织修复的功效。愈合效果归因于其生物活性化合物,增强血管和组织再生。安全性评估,包括急性毒性、眼部刺激和皮肤刺激试验,证实该油无毒无刺激,在小鼠中,高达70 mL/kg(口服)和6 mL/kg(腹腔)的剂量均未观察到死亡。对家兔皮肤和直肠粘膜均无不良反应。主要酚类成分为奎宁酸(4.09 μg)、香豆素(1.60 μg)和橙皮苷(0.50 μg),对其抗氧化和抗炎具有重要作用。多不饱和脂肪酸与饱和脂肪酸的高比率(3.10)进一步强调了它的治疗和营养意义。本研究确立了棘豆油作为一种有前途的、天然的、安全的生物工程皮肤应用候选者。通过解决其伤口愈合机制和安全性的经验数据差距,该研究支持其用于开发可持续的、临时的和永久的再生医学工程皮肤替代品。未来的研究应集中在阐明其分子途径,优化提取技术和评估长期临床安全性。
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引用次数: 0
Digestibility, nutritional quality and microbial safety of hybrid beef patties containing texturized pea protein 混合豌豆蛋白牛肉饼的消化率、营养品质及微生物安全性
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.fbp.2025.11.021
Joanna Tkaczewska , Michael Leeming , Xinyu Miao , Robyn Warner
This study assessed the digestibility, nutritional quality, and microbial safety of hybrid meat products combining texturized pea protein and beef, compared to pure beef. In vitro digestion showed slightly lower protein solubility in hybrid products (51.1 %) than in beef (59.2 %). However, proteomic analysis revealed that pea proteins influence the digestion of meat proteins, altering peptide release kinetics and challenging the assumption that hybrid products are less digestible. Microbial analysis showed no differences in bacterial counts, but yeast and mold growth were higher in hybrid products, potentially reducing shelf-life. These findings offer a novel perspective on hybrid protein digestion, demonstrating that plant proteins do not simply hinder digestibility but modify enzymatic breakdown. This study highlights the complexity of mixed-protein digestion and contributes to a deeper understanding of hybrid food systems.
与纯牛肉相比,本研究评估了纹理化豌豆蛋白与牛肉混合肉制品的消化率、营养品质和微生物安全性。体外消化表明,杂交产物的蛋白质溶解度(51.1 %)略低于牛肉(59.2 %)。然而,蛋白质组学分析显示,豌豆蛋白影响肉类蛋白的消化,改变肽释放动力学,挑战杂交产品不易消化的假设。微生物分析显示,细菌数量没有差异,但酵母和霉菌的生长在杂交产品中更高,可能会缩短保质期。这些发现为杂交蛋白消化提供了一个新的视角,表明植物蛋白不仅阻碍消化,而且改变酶分解。这项研究突出了混合蛋白质消化的复杂性,并有助于对混合食物系统的更深层次的理解。
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Food and Bioproducts Processing
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