This study aimed to prepare soybean protein isolate hydrolysate-Schisandra chinensis polysaccharide complex (SPH-SCP) to obtain a series of Pickering emulsions for the delivery of quercetin (QUE). Fourier transform infrared spectroscopy, fluorescence spectroscopy and dynamic interfacial tension analysis confirmed that SCP and SPH form complexes through hydrogen bonding, hydrophobic interactions, and electrostatic interactions, leading to alterations in the structure of SPH and a reduction in interfacial tension. Compared with emulsion stabilized by SPH, the QUE-loaded Pickering emulsion stabilized by SPH-SCP (SPH-SCP/QUE) exhibited smaller particles (517.13 ± 1.62 nm), higher absolute values of zeta potential (−41.82 ± 1.13 mV), and improved apparent viscosities, modulus, encapsulation efficiency (91.65 ± 0.25 %), loading ability (89.93 ± 0.15 %) and stability. These properties collectively reduced the release of free fatty acids (5.09 ± 0.14 %) and QUE (17.85 ± 3.13 %), thereby enhancing the bioaccessibility of QUE. In addition, the prepared SPH-0.1 %SCP/QUE emulsion exhibited strong antibacterial activity against Staphylococcus aureus. Intake of sugar and protein production assays revealed that SPH-0.1 %SCP/QUE Pickering emulsion inhibited the carbohydrate metabolism of Staphylococcus aureus by binding pyruvate kinase. Ultraviolet spectroscopy, Fourier transform infrared spectroscopy, fluorescence spectroscopy, isothermal titration calorimetry, molecular docking and molecular dynamics simulations assays confirmed that there were strong binding affinity and interaction between QUE and pyruvate kinase. In conclusion, the Pickering emulsions fabricated in this study displayed robust QUE encapsulation capability and potent antibacterial activity. These findings demonstrate the considerable potential of emulsions for applications such as the encapsulation of bioactive compounds, intestinal health, and food preservation.
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