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Preparation and characterization of quercetin-loaded antibacterial Pickering emulsion stabilized by soy protein isolate hydrolysate-Schisandra Chinensis polysaccharide 大豆分离蛋白水解物-五味子多糖稳定槲皮素抗菌酸洗乳的制备与表征
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-14 DOI: 10.1016/j.fbp.2026.01.007
Jingwen Bai, Bin Yu, Chi Wei, Haodong Xu, Huifang Cao, Yu Yang
This study aimed to prepare soybean protein isolate hydrolysate-Schisandra chinensis polysaccharide complex (SPH-SCP) to obtain a series of Pickering emulsions for the delivery of quercetin (QUE). Fourier transform infrared spectroscopy, fluorescence spectroscopy and dynamic interfacial tension analysis confirmed that SCP and SPH form complexes through hydrogen bonding, hydrophobic interactions, and electrostatic interactions, leading to alterations in the structure of SPH and a reduction in interfacial tension. Compared with emulsion stabilized by SPH, the QUE-loaded Pickering emulsion stabilized by SPH-SCP (SPH-SCP/QUE) exhibited smaller particles (517.13 ± 1.62 nm), higher absolute values of zeta potential (−41.82 ± 1.13 mV), and improved apparent viscosities, modulus, encapsulation efficiency (91.65 ± 0.25 %), loading ability (89.93 ± 0.15 %) and stability. These properties collectively reduced the release of free fatty acids (5.09 ± 0.14 %) and QUE (17.85 ± 3.13 %), thereby enhancing the bioaccessibility of QUE. In addition, the prepared SPH-0.1 %SCP/QUE emulsion exhibited strong antibacterial activity against Staphylococcus aureus. Intake of sugar and protein production assays revealed that SPH-0.1 %SCP/QUE Pickering emulsion inhibited the carbohydrate metabolism of Staphylococcus aureus by binding pyruvate kinase. Ultraviolet spectroscopy, Fourier transform infrared spectroscopy, fluorescence spectroscopy, isothermal titration calorimetry, molecular docking and molecular dynamics simulations assays confirmed that there were strong binding affinity and interaction between QUE and pyruvate kinase. In conclusion, the Pickering emulsions fabricated in this study displayed robust QUE encapsulation capability and potent antibacterial activity. These findings demonstrate the considerable potential of emulsions for applications such as the encapsulation of bioactive compounds, intestinal health, and food preservation.
本研究旨在制备大豆分离水解蛋白-五味子多糖复合物(SPH-SCP),以获得一系列用于递送槲皮素(QUE)的皮克林乳剂。傅里叶变换红外光谱、荧光光谱和动态界面张力分析证实SCP与SPH通过氢键、疏水相互作用和静电相互作用形成配合物,导致SPH结构发生改变,界面张力降低。与乳液稳定,相比,QUE-loaded皮克林乳液稳定的SPH-SCP (SPH-SCP /是)表现出更小的微粒(517.13 ±1.62  海里),更高的绝对值电动电势(−41.82 ±1.13  mV),和改进的表观粘度,模量、封装效率(91.65 ±0.25  %),装载能力(89.93 ±0.15  %)和稳定性。这些特性共同减少了游离脂肪酸(5.09 ± 0.14 %)和QUE(17.85 ± 3.13 %)的释放,从而提高了QUE的生物可及性。此外,制备的SPH-0.1 %SCP/QUE乳剂对金黄色葡萄球菌具有较强的抗菌活性。糖的摄入和蛋白质的产生实验表明,SPH-0.1 %SCP/QUE Pickering乳剂通过结合丙酮酸激酶抑制金黄色葡萄球菌的碳水化合物代谢。紫外光谱、傅里叶变换红外光谱、荧光光谱、等温滴定量热法、分子对接和分子动力学模拟分析证实了QUE与丙酮酸激酶之间存在较强的结合亲和力和相互作用。总之,本研究制备的皮克林乳剂具有强大的QUE包封能力和有效的抗菌活性。这些发现表明,乳剂在生物活性化合物的包封、肠道健康和食品保存等方面具有相当大的应用潜力。
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引用次数: 0
Developing anthocyanin-loaded aerogels from purple sweet potatoes using supercritical carbon dioxide drying 超临界二氧化碳干燥法制备紫红薯花青素气凝胶
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-02-03 DOI: 10.1016/j.fbp.2026.02.002
Gabriel Laquete De Barros , Arda Tuhanioglu , Sumanjot Kaur , Márcia Vizzotto , Cesar Valmor Rombaldi , Leonardo Nora , Ali Ubeyitogullari
This study developed bioactive-rich aerogels from purple sweet potato powder (PSPP) and corn starch (CS) via supercritical carbon dioxide (SC-CO2) drying. The aerogels were prepared at varying PSPP concentrations (0 %, 25 %, 50 %, 75 %, and 100 %) with a complementary mixture of CS. Additionally, the purple sweet potato residue (PSPR) obtained after SC-CO2 extraction (30 MPa, 35 °C, and 20 % cosolvent concentration) was evaluated for its aerogel-forming capacity. Once aerogels were developed, anthocyanins were loaded in all samples (PSPP and PSPR), then compared in terms of total phenolic content (TPC), anthocyanin content (ANT), and antioxidant activities (DPPH, ABTS, FRAP, and ORAC). The 50 % PSPP and 50 % CS mixture loaded with anthocyanin extract (PSPP50-CS50-ANT) aerogel showed the highest ANT (1403 ± 10 mg C3G/100 g) and TPC (2314 ± 12 mg GAE/100 g). The developed aerogels showed a surface area of 129.4 m²/g. These findings reveal complementary functional behaviors across formulations and highlight the potential of purple sweet potato-based aerogels as eco-friendly carriers for natural colorants and nutraceutical ingredients.
以紫薯粉(PSPP)和玉米淀粉(CS)为原料,采用超临界二氧化碳(SC-CO2)干燥法制备了富含生物活性的气凝胶。以不同浓度的PSPP(0 %,25 %,50 %,75 %和100 %)与CS的互补混合物制备气凝胶。此外,还对SC-CO2萃取(30 MPa, 35°C, 20 %共溶剂浓度)后得到的紫薯渣(PSPR)进行了气凝胶形成能力的评价。气凝胶制备完成后,在所有样品(PSPP和PSPR)中加载花青素,然后比较总酚含量(TPC)、花青素含量(ANT)和抗氧化活性(DPPH、ABTS、FRAP和ORAC)。50 % PSPP和50 % CS混合物富含花青素提取(PSPP50-CS50-ANT)气凝胶显示最高的蚂蚁(1403 ± C3G 10毫克/ 100 g)和TPC(2314 ± 100 GAE 毫克/克)。制备的气凝胶的表面积为129.4 m²/g。这些发现揭示了不同配方的互补功能行为,并突出了紫红薯气凝胶作为天然着色剂和营养成分的环保载体的潜力。
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引用次数: 0
Advancements, challenges, and opportunities of active bio-composite films for food packaging and preservation: A comprehensive review 食品包装和保鲜用活性生物复合膜的进展、挑战和机遇:综述
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-02-12 DOI: 10.1016/j.fbp.2026.02.008
Taame Berhanu Teklemedhin, Yanzhu Guo, Haiming Li
Food packaging widely uses conventional plastics due to their outstanding mechanical performance, water resistance, and barrier properties. Approximately 300 million metric tons of petroleum-based plastics are produced annually, with more than 60 % of the total plastic used in the food packaging sector. However, the toxicity and non-biodegradability of conventional plastics lead to serious environmental and health problems that require solutions. Therefore, researchers are developing sustainable alternatives in the form of active bio-composite films that are synthesized from renewable and biodegradable resources. This review article discusses the latest developments in active bio-composite films made from different natural materials like polysaccharides, proteins, and biodegradable synthetic polymers, which include active ingredients (like plant extracts and metal nanoparticles) for food packaging and preservation. In addition, smart and intelligent bio-composite films for food packaging applications are highlighted; the limitations of active bio-composite films are discussed, and suggestions for future research and innovation are provided. The incorporation of nanomaterials such as nanocellulose and metallic nanoparticles can improve the synergistic mechanical and barrier properties of bio-composite films. In addition, the addition of active agents in the film-forming mixture can also improve the antimicrobial and antioxidant properties of the films. Thus, active bio-composite films represent an alternative for packaging food that shows promise in sustainability. Given that these films are biodegradable and fit into the environment, biodiversity, human health, and the circular economy.
由于传统塑料具有优异的机械性能、耐水性和阻隔性,因此食品包装广泛使用传统塑料。每年大约生产3亿吨石油基塑料,其中60%以上的塑料用于食品包装行业 。然而,传统塑料的毒性和不可生物降解性导致了需要解决的严重环境和健康问题。因此,研究人员正在开发以可再生和可生物降解资源合成的活性生物复合膜形式的可持续替代品。本文综述了以多糖、蛋白质和可生物降解的合成聚合物等不同天然材料为原料,利用植物提取物和金属纳米颗粒等有效成分制备食品包装和保鲜用活性生物复合膜的最新进展。此外,还重点介绍了用于食品包装的智能生物复合薄膜;讨论了活性生物复合膜的局限性,并对今后的研究和创新提出了建议。纳米纤维素和金属纳米颗粒等纳米材料的掺入可以提高生物复合膜的协同力学和屏障性能。此外,在成膜混合物中加入活性剂也可以提高膜的抗菌和抗氧化性能。因此,活性生物复合薄膜代表了一种包装食品的替代方案,显示出可持续发展的前景。鉴于这些薄膜是可生物降解的,适合环境、生物多样性、人类健康和循环经济。
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引用次数: 0
Continuous fibers with meat-like fibrous morphology based on ionically cross-linked SA/CG/SPI: A novel delivery material for efficient encapsulation and release of oral Lactobacillus plantarum 基于离子交联SA/CG/SPI的具有肉状纤维形态的连续纤维:一种有效封装和释放口服植物乳杆菌的新型递送材料
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-16 DOI: 10.1016/j.fbp.2026.01.010
Xinbin Ji , Zheng Li , Yulan Wang, Lei Han, Fucheng Guan, Jing Guo, Xin Zhang
To mitigate probiotic inactivation and low colonization during oral delivery, continuous fibers with meat-like fibrous morphology were fabricated from a sodium alginate/karaya gum/soy protein isolate (SA/CG/SPI) matrix to gently encapsulate Lactobacillus plantarum and enable controlled release. Network density was tuned by wet spinning followed by Ca²⁺-mediated crosslinking. The effects of two calcium sources—calcium chloride (CaCl2) and calcium gluconate—on microstructure, mechanical/thermal stability, and encapsulation-release behavior were systematically evaluated. The results indicate that CaCl2, which dissociates rapidly, forms a denser crosslinking gradient with smaller pore sizes, effectively balancing physical barrier properties and thermal protection. This leads to a stable, sustained release in a simulated in vitro digestive environment (PBS). By contrast, the slower dissociation of calcium gluconate generated a looser network with inferior overall performance. These differences were corroborated by morphological/spectroscopic analyses and mechanical testing. After comprehensive optimization, the best comprehensive performance was achieved under 2 % CaCl2 conditions: the tensile strength was approximately 139 kPa, the elongation at break was approximately 59.3 %, the encapsulation rate was up to approximately 85.4 %, and the cumulative release amount was approximately 7.99 log (CFU·g−1) in PBS within 8 h, with a stable release trend after about 5 h. This strategy offers a scalable materials-process framework for probiotic functional foods and oral delivery systems.
为了减轻口服给药过程中益生菌的失活和低定植,用海藻酸钠/karaya gum/大豆分离蛋白(SA/CG/SPI)基质制备了具有肉样纤维形态的连续纤维,以轻柔地包裹植物乳杆菌并使其可控释放。通过湿纺丝调整网络密度,然后用Ca 2 +介导交联。系统评价了氯化钙(CaCl2)和葡萄糖酸钙两种钙源对其微观结构、机械/热稳定性和包封释放行为的影响。结果表明,CaCl2快速解离,形成更密集的交联梯度和更小的孔径,有效地平衡了物理屏障性能和热防护。这导致在模拟体外消化环境(PBS)中稳定,持续释放。相比之下,葡萄糖酸钙较慢的解离产生较松散的网络,整体性能较差。这些差异被形态学/光谱分析和力学测试证实。综合优化后,在2 % CaCl2条件下获得最佳的综合性能:抗拉强度约为139 kPa,断裂伸长率约为59.3 %,包封率约为85.4 %,在PBS中8 h内的累积释放量约为7.99 log (CFU·g−1),约5 h后释放趋于稳定。该策略为益生菌功能食品和口服给药系统提供了可扩展的材料-工艺框架。
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引用次数: 0
Xanthan gum production from sugarcane bagasse and its potential for biodegradable food packaging films 甘蔗渣生产黄原胶及其在生物可降解食品包装膜方面的潜力
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-13 DOI: 10.1016/j.fbp.2026.01.008
Maria Rita Meyer Ferraz da Costa , Enio Nazaré de Oliveira Junior , Marali Vilela Dias , Lisbeth Garbrecht Thygesen , Gustavo Henrique Denzin Tonoli , Anand Ramesh Sanadi , Mario Guimarães Junior
Growing environmental concerns over plastic pollution have intensified the search for sustainable alternatives in food packaging. However, the high cost of biopolymers remains a barrier to large-scale adoption. This study addresses this gap by investigating the use of sugarcane bagasse, an abundant agro-industrial residue in Minas Gerais (Brazil), as a low-cost substrate for xanthan gum (XG) production. The objective was to evaluate the physicochemical properties of lab-produced XG and compare them to commercial XG, assessing their suitability for incorporation into biodegradable packaging films. In this sense, the developed gum was characterized for apparent and intrinsic viscosities, molecular weight, chemical/functional groups (FTIR), thermal properties (TGA and DSC), and elemental analysis (C, H, O, N and S). Fermentation of sugarcane bagasse extract (6 % w/v) with Xanthomonas campestris CCT 5268 at 28 °C for 120 h yielded 2.07 g/L of gum. The biopolymer exhibited pseudoplastic behavior, with an apparent viscosity of 135 mPa·s at 25° C and 25 s⁻¹ . Thermal analysis revealed degradation temperatures of 290º C (lab-produced) and 300º C (commercial), indicating comparable stability. The molecular weight of the lab-produced gum was 1238 kDa, surpassing the commercial sample (1018 kDa). FTIR and elemental analyses confirmed structural similarity between both samples, with minor differences attributed to the fermentation substrate. These findings demonstrate the feasibility of valorizing agro-industrial waste for the production of functional biopolymers, supporting the development of cost-effective and sustainable packaging materials.
对塑料污染日益增长的环境担忧,促使人们加大了对可持续食品包装替代品的寻找力度。然而,生物聚合物的高成本仍然是大规模采用的障碍。本研究通过研究甘蔗渣作为黄原胶(XG)生产的低成本底物的使用,解决了这一差距。甘蔗渣是巴西米纳斯吉拉斯州的一种丰富的农业工业残留物。目的是评估实验室生产的XG的物理化学性质,并将其与商业XG进行比较,评估其纳入可生物降解包装膜的适用性。从这个意义上说,开发的胶的表观和特性粘度,分子量,化学/官能团(FTIR),热性能(TGA和DSC)和元素分析(C, H, O, N和S)进行了表征。甘蔗渣提取物(6 % w/v)与油菜黄单胞菌CCT 5268在28℃下发酵120 h,产胶量为2.07 g/L。该生物聚合物表现出假塑性行为,在25°C和25 s的表观粘度为135 mPa·s⁻¹ 。热分析显示降解温度为290ºC(实验室生产)和300ºC(商业),表明相当的稳定性。实验室生产的口香糖分子量为1238 kDa,超过了市售样品(1018 kDa)。FTIR和元素分析证实了两种样品的结构相似,发酵底物的差异较小。这些发现证明了利用农业工业废弃物生产功能性生物聚合物的可行性,支持开发具有成本效益和可持续性的包装材料。
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引用次数: 0
Multi-objective optimization of solid-liquid stirred tank with fractal impeller and baffle for food processing 食品加工分形叶轮挡板固液搅拌槽的多目标优化
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-08 DOI: 10.1016/j.fbp.2026.01.002
Deyin Gu, Tao Peng, Changshu Li, Li Wen, Yinghua Song, Haifeng Gong
This work applied fractal theory to the structure design of the impeller and baffle in the solid-liquid stirred reactors, proposing the coupled use of the fractal-structure impeller and fractal-structure baffle to improve the suspension quality of solid particles. Meanwhile, an optimization framework consisting of one-hot encoding, BP neural network, NSGA-II, and TOPSIS decision making was constructed to achieve an optimal balance among the solid suspension degree, local solid holdup distribution, and power consumption. The findings showed that the optimal case increased the solid suspension degree by 1.41 %, decreased the power consumption by 10.37 %, increased local solid holdup Ch1 (r/R = 0.8, z/H = 0.9) by 19.50 %, and decreased local solid holdup Ch5 (r/R = 0.8, z/H = 0.1) by 2.32 % compared to the base case. This indicated that the optimal case could effectively reduce the stirring power consumption, decrease the axial concentration gradient of solid particles, and enhance the solid suspension degree. In addition, the numerical simulation results further confirmed that the optimal case could increase the fluid circulation velocity, reduce the low-velocity stagnant zone, enhance the turbulent kinetic energy, mitigate the deposition of solid particles, and improve the mixing quality of solid-liquid two-phase compared with the base case. This study provides a valuable structural optimization approach for enhancing the performance of solid-liquid stirred reactors.
本文将分形理论应用于固液搅拌反应器中叶轮和挡板的结构设计,提出了分形结构叶轮和分形结构挡板的耦合使用,以提高固体颗粒的悬浮质量。同时,构建了由单热编码、BP神经网络、NSGA-II和TOPSIS决策组成的优化框架,以实现固体悬浮度、局部固体含率分布和功耗之间的最优平衡。结果表明:与基本情况相比,优化后的悬浮度提高了1.41 %,能耗降低了10.37 %,局部固含率Ch1 (r/ r = 0.8, z/H = 0.9)提高了19.50 %,局部固含率Ch5 (r/ r = 0.8, z/H = 0.1)降低了2.32 %。结果表明,该优化方案能够有效降低搅拌功率消耗,减小固体颗粒轴向浓度梯度,提高固体悬浮度。此外,数值模拟结果进一步证实,与基本情况相比,最优情况可以增加流体循环速度,减少低速停滞区,增强湍流动能,减轻固体颗粒沉积,改善固液两相混合质量。该研究为提高固液搅拌反应器的性能提供了一种有价值的结构优化方法。
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引用次数: 0
From herbs to high-performance adsorbents: Chemical activation with orthophosphoric acid as a method for obtaining tailored activated biocarbons for simultaneous removal of ionic polymers from two-component systems 从草药到高性能吸附剂:用正磷酸进行化学活化,以获得定制的活性活性炭,同时从双组分体系中去除离子聚合物
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fbp.2026.01.014
Marlena Groszek , Małgorzata Wiśniewska , Dariusz Sternik , Piotr Nowicki
Herbal residues, specifically mint and lemon balm stems, were successfully converted into carbonaceous adsorbents via an eco-friendly method involving chemical activation with H₃PO₄. This low-temperature and straightforward transformation of waste biomass yielded biocarbons that were subjected to detailed physicochemical analysis, including evaluation of their pore structure, surface chemistry, electrokinetic properties, and thermal stability. The main aim of this study was to investigate the adsorption performance of these materials toward ionic polymers with different chemical characteristics – the anionic poly(acrylic acid) and the cationic polyethyleneimine – as well as to assess their effectiveness in the simultaneous removal of structurally diverse organic pollutants from aqueous solutions.
The resulting activated biocarbons were characterised by a moderately developed surface area (950–1146 m²/g), a strongly mesoporous structure (with an average pore diameter of 4.63–5.12 nm), and surface functionalities enriched with both acidic and basic groups, with a total content ranging from 0.794 to 0.987 mmol/g. This unique combination of textural and chemical properties makes them highly effective for capturing ionic polymer contaminants from aqueous systems. The maximum adsorption capacities reached 430.3 mg/g for poly(acrylic acid) and 495.9 mg/g for polyethyleneimine, which is the highest amount of these compounds adsorbed on carbonaceous materials reported in the literature so far.
It was further observed that the presence of one polymer reduced the adsorption efficiency of the other. Regeneration experiments indicated that polyethyleneimine could be removed with water only to a limited extent (17.4–20.5 %), while NaOH proved to be the most suitable desorbing agent for poly(acrylic acid) (64.5–71.8 %). Overall, the study demonstrates that activated biocarbons derived from herbal waste, with carefully engineered physicochemical properties, provide an effective and reusable solution for eliminating various ionic polymer contaminants from wastewater.
草药残留物,特别是薄荷和柠檬香草茎,通过一种环保的方法,用H₃PO₄化学活化,成功地转化为碳质吸附剂。这种对废弃生物质的低温直接转化产生了生物碳,并对其进行了详细的物理化学分析,包括对其孔隙结构、表面化学、电动特性和热稳定性的评估。本研究的主要目的是研究这些材料对具有不同化学特性的离子聚合物(阴离子聚丙烯酸和阳离子聚乙烯亚胺)的吸附性能,并评估它们同时去除水溶液中结构不同的有机污染物的有效性。所得活性炭具有中等发育的比表面积(950 ~ 1146 m²/g)、强介孔结构(平均孔径为4.63 ~ 5.12 nm)和富含酸性和碱性基团的表面官能团(总含量为0.794 ~ 0.987 mmol/g)。这种独特的结构和化学性质的组合使它们非常有效地从水系统中捕获离子聚合物污染物。对聚丙烯酸和聚亚胺的最大吸附量分别达到430.3 mg/g和495.9 mg/g,是目前文献报道的这两种化合物在含碳材料上的最高吸附量。进一步观察到,一种聚合物的存在降低了另一种聚合物的吸附效率。再生实验表明,水对聚乙烯亚胺的去除率有限(17.4 ~ 20.5 %),而NaOH是最适合聚丙烯酸的解吸剂(64.5 ~ 71.8 %)。总体而言,该研究表明,从草药废弃物中提取的活性炭具有精心设计的物理化学特性,可为消除废水中的各种离子聚合物污染物提供有效且可重复使用的解决方案。
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引用次数: 0
New insights into the effects of sugars and cultivars on the mass transfer, quality, and health properties of osmotically dehydrated peach 糖和品种对渗透脱水桃传质、品质和健康特性影响的新认识
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-23 DOI: 10.1016/j.fbp.2026.01.015
Lara Salvañal , Julieta Gabilondo , Graciela Beatriz Corbino , Claudio O. Budde , María Valeria Lara
The effects of three osmotic agents (glucose, sorbitol and sucrose) and four cultivars of peach were analysed in osmotically dehydrated fruit slices followed by convective air drying (OD+D). A comprehensive analysis of mass transfer parameters, and organoleptic, nutritional and nutraceutical properties was conducted, which included, for the first time, mineral composition and antimicrobial and enzymatic inhibitory activities in peach OD+D slices. Osmotic pretreatment improved the inhibition of protease, α-amylase and α-amyloglucosidase activities and the contents of tannins, flavonoids, phenolics and calcium in the OD+D treatment compared with those in the fresh fruit. Thus, OD+D adds value to a natural product, rendering peach slices that can be used in industry or consumed as snacks, with a sugar content equivalent to or lower than that of other fruits. Moreover, higher antimicrobial capacity and metabolite and antioxidant capacity retention were observed in OD+D than in exclusively hot-air-dried fruits. Clustering analysis of the OD+D slices displayed that the samples were grouped on the basis of the cultivar but not by the osmotic agents used for pretreatment, which is in line with the results of the statistical analysis, which revealed that the color; antioxidant capacity; ascorbic acid, carotenoid, phenolic, flavonoid, and protein contents; inhibition of α-amylase and protease activity; and bacterial growth of the OD+D samples were independent of the osmotic agents used. The results obtained demonstrate that the choice of cultivar is a key determinant of the properties of the resulting dehydrated peach and reveal new benefits of osmotic pretreatment prior to convective-drying.
分析了3种渗透剂(葡萄糖、山梨醇和蔗糖)和4个品种的桃果片在对流空气干燥(OD+D)后的渗透脱水效果。本文首次对桃OD+D切片的传质参数、感官、营养和营养保健性质进行了综合分析,其中包括矿物质组成、抗菌活性和酶抑制活性。与鲜果相比,渗透预处理提高了OD+D处理对蛋白酶、α-淀粉酶和α-淀粉葡糖苷酶活性的抑制作用以及单宁、黄酮类、酚类物质和钙的含量。因此,OD+D增加了天然产品的价值,使桃子片可以用于工业或作为零食食用,其含糖量相当于或低于其他水果。此外,OD+D处理的抗微生物能力、代谢物和抗氧化能力均高于纯热风干果。对OD+D切片的聚类分析表明,样品是按品种分组的,而不是按预处理所用渗透剂分组的,这与统计分析的结果一致,显示颜色;抗氧化能力;抗坏血酸、类胡萝卜素、酚类、类黄酮和蛋白质含量;α-淀粉酶和蛋白酶活性的抑制;OD+D样品的细菌生长与所使用的渗透剂无关。结果表明,品种的选择是水蜜桃脱水特性的关键决定因素,并揭示了对流干燥前渗透预处理的新益处。
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引用次数: 0
Valorization of Amazonian biodiversity: Comparative analysis of conventional and ultrasonic techniques for preservation of bioactive compounds in nectar made from nance (Byrsonima crassifolia) fruit 亚马逊生物多样性的增值:传统和超声波技术保存南南树果实花蜜中生物活性化合物的比较分析
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-23 DOI: 10.1016/j.fbp.2026.01.006
Francisca das Chagas do Amaral Souza , Leonardo Saders Moura , Jaime Paiva Lopes Aguiar , Midori Nakamura Marques
This study investigated the impact of the use of ultrasound on the processing of the nectar made from nance (B. crassifolia) fruit, which is rich in bioactive compounds, and compared pasteurization techniques (70, 80 and 90 °C) with emerging technologies assisted by ultrasound (40, 50 and 60 °C). The objective was to evaluate the preservation of quality, the inactivation of enzymes (polyphenoloxidase - PPO and peroxidase - POD) and the bioaccessibility of its compounds. The physicochemical and microbiological characteristics and the concentrations of the bioactive compounds (anthocyanins, flavonoids, antioxidants, phenolic compounds and β-carotene) were tested. The results indicate that thermosonication, at moderate temperatures, preserved the essential physicochemical properties of the nectar, maintaining soluble solids, pH and titratable acidity. Pasteurization at 90 °C was more effective for inactivating PPO, while thermosonication at 50 °C showed special POD inactivation. Turbidity values remained within the recommended range for nectar quality under both treatments. It is concluded that, when choosing between the techniques, one should consider the thermal stability of the target enzymes, seeking a balance between enzyme inactivation and the preservation of product quality. Thermosonication is, however, a viable alternative for the processing of nectar made from the fruit of B. crassifolia and preserves its essential properties.
本研究研究了超声波对富含生物活性化合物的南楠果花蜜加工的影响,并比较了巴氏灭菌技术(70、80和90°C)和超声辅助下的新兴技术(40、50和60°C)。目的是评估其质量的保存,酶(多酚氧化酶- PPO和过氧化物酶- POD)的失活及其化合物的生物可及性。测定了花青素、黄酮类化合物、抗氧化剂、酚类化合物和β-胡萝卜素等生物活性物质的理化、微生物学特性和浓度。结果表明,在适当的温度下,热超声保存了花蜜的基本物理化学性质,保持了可溶性固形物、pH和可滴定酸度。90°C的巴氏灭菌对PPO失活更有效,而50°C的热超声对POD失活更有效。在两种处理下,浊度值都保持在甘露质量的推荐范围内。综上所述,在选择两种技术时,应考虑目标酶的热稳定性,在酶失活和保持产品质量之间寻求平衡。然而,热超声处理是一种可行的替代方法,可以处理由金合欢果实制成的花蜜,并保留其基本特性。
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引用次数: 0
Mitigation of gold nanoparticle fouling in continuous microreactors 连续微反应器中金纳米颗粒污染的缓解
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.fbp.2026.01.021
Joseph Carver , YM John Chew , Semali Perera , Sooyeon Moon , Sourav Chatterjee
Gold nanoparticles (Au-NPs) are gaining interest in food processing, with applications ranging from antimicrobial food packaging to sensing and detection of foodborne pathogens. However, scaling their synthesis in a continuous flow system remains a formidable challenge, not only requiring precise control over particle size, process stability and high throughput, but also facing the persistent problem of surface fouling. In flow-based systems, this fouling typically originates at the microreactor inlet, where gold nuclei deposit on channel walls, triggering uncontrolled particle growth and, in extreme cases, complete blockage. In this study, Au-NPs were synthesized via citrate reduction of tetrachloroauric acid in a segmented flow microreactor, with antifouling strategies explored through chemical surface modification. Coating reactor walls with silica nanoparticles and subsequent silanization created a superhydrophobic interface, which showed some success in mitigating fouling. However, the trade-off between fouling prevention and product control remained evident. Importantly, we also show that fouled reactors can be effectively cleaned with aqua regia, a practical approach to prolong continuous operation beyond single use. These findings highlight surface fouling as a critical barrier in scalable Au-NP synthesis and provide insights into the broader challenges of optimising nanoparticle production in continuous flow.
金纳米颗粒(Au-NPs)在食品加工中越来越受到关注,其应用范围从抗菌食品包装到食源性病原体的传感和检测。然而,在连续流系统中扩展它们的合成仍然是一个巨大的挑战,不仅需要精确控制粒度,工艺稳定性和高通量,而且还面临持续存在的表面结垢问题。在基于流动的系统中,这种污染通常起源于微反应器入口,金核沉积在通道壁上,引发不受控制的颗粒生长,在极端情况下,完全堵塞。本研究采用四氯金酸柠檬酸还原法在分段流微反应器中合成Au-NPs,并通过化学表面改性探索防污策略。在反应器壁上涂覆二氧化硅纳米颗粒和随后的硅烷化形成了超疏水界面,这在一定程度上成功地减轻了污染。然而,污垢预防和产品控制之间的权衡仍然很明显。重要的是,我们还表明,污染的反应器可以用王水有效地清洗,这是一种延长单次使用后连续运行的实用方法。这些发现强调了表面污垢是可扩展的Au-NP合成的关键障碍,并为优化连续流中纳米颗粒生产的更广泛挑战提供了见解。
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引用次数: 0
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Food and Bioproducts Processing
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