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Environmental carbon tetrachloride exposure disrupts the liver structure and metabolic detoxification function in mice via p38MAPK/NF-κB/NLRP3 pathway 环境四氯化碳暴露通过p38MAPK/NF-κB/NLRP3途径破坏小鼠肝脏结构和代谢解毒功能
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-04-11 DOI: 10.1080/09540105.2022.2060192
Yuanyuan Wei, Danyang Ma, Yimeng Fan, Chen Gao, Qingtao Wang, Yanmeng Yuan, Yan-nan Zhang, J. Han, Zhihui Hao
ABSTRACT We investigated the effects of carbon tetrachloride (CCl4) exposure on liver detoxification. We confirmed that 0.2%-0.8% CCl4 decreased the liver carboxylesterase (CarE), lactate dehydrogenase (LDH) activity and total protein (TP) levels, while 0.2% and 0.8% CCl4 increased the liver acetylcholine esterase (AChE) activity (P< 0.05). It was observed0.4%-0.8% CCl4 increased serum glutathione S-transferase (GST) and catalase (CAT) activity, 0.1%-0.8% CCl4 increased the level of malondialdehyde (MDA), and 0.2% CCl4 increased the level of total superoxide dismutase (T-SOD). The expression of nod-like receptor protein 3 (NLRP3), interleukin-1β (IL-1β), and tumour necrosis factor-α (TNF-α) were significantly elevated in 0.2%-0.8% CCl4 exposure (P< 0.05). The expression of p38MAPK, gasdermin D (GSDMD), and nuclear factor kappa-B kinase (IKK) was decreased in 0.1%-0.8% CCl4 exposure, while the apoptosis was not statistically different in all groups. This indicates that 0.1% CCl4 exposure could damage the liver structure and detoxification function via p38MAPK/NF-κB/NLRP3 pathway. GRAPHICAL ABSTRACT
摘要:我们研究了四氯化碳(CCl4)暴露对肝脏解毒的影响。结果表明,0.2% ~ 0.8% CCl4降低了肝脏羧酸酯酶(CarE)、乳酸脱氢酶(LDH)活性和总蛋白(TP)水平,0.2%和0.8% CCl4提高了肝脏乙酰胆碱酯酶(AChE)活性(P< 0.05)。0.4% ~ 0.8% CCl4可提高血清谷胱甘肽s转移酶(GST)和过氧化氢酶(CAT)活性,0.1% ~ 0.8% CCl4可提高丙二醛(MDA)水平,0.2% CCl4可提高总超氧化物歧化酶(T-SOD)水平。暴露于0.2% ~ 0.8% CCl4时,淋巴结样受体蛋白3 (NLRP3)、白细胞介素-1β (IL-1β)、肿瘤坏死因子-α (TNF-α)的表达显著升高(P< 0.05)。在0.1% ~ 0.8% CCl4暴露时,p38MAPK、气皮蛋白D (GSDMD)和核因子κ b激酶(IKK)的表达降低,而凋亡在各组间无统计学差异。这表明0.1%的CCl4暴露可通过p38MAPK/NF-κB/NLRP3通路破坏肝脏结构和解毒功能。图形抽象
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引用次数: 0
Monoclonal antibody-based immunoassay for the specific quantification of licochalcone A: an active chalcone in licorice 甘草查尔酮A:甘草中的一种活性查尔酮
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-04-04 DOI: 10.1080/09540105.2022.2053948
S. Krittanai, Piriyakorn Pichetpongtorn, S. Sakamoto, W. Putalun
ABSTRACT Licochalcone A (LicoA), which is found in the root of Chinese licorice (Glycyrrhiza inflata Batalin), has been reported as an effective anti-microbial and anti-inflammation and was approved for the treatment of rosacea and acne in clinical therapeutic. To develop a monoclonal antibody against LicoA, a mannich reaction hapten conjugate was used for immunization, followed by the hybridoma technique. Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of LicoA was developed using a constructed antibody. The assay validation results were highly specific for the target compound, but minimally cross-reactive with the structure-related substances. After optimal conditions, the detection limit was 4.32 ng/mL and the quantification limit was 6.84–107.21 ng/mL. As a result, the developed ELISA was applied to determine the concentration of LicoA in raw licorice and marketed samples. This assay will aid in the quality control of licorice and derived products, as it can be used to identify plant species and determine bioactive markers.
甘草查尔酮A (LicoA)是中国甘草(Glycyrrhiza inflata Batalin)根中发现的一种有效的抗微生物和抗炎症物质,已被批准用于治疗酒渣鼻和痤疮的临床治疗。采用曼尼希反应半抗原偶联物进行免疫,然后采用杂交瘤技术制备抗LicoA单克隆抗体。建立了酶联免疫吸附法(ELISA),利用构建的抗体定量测定LicoA。实验验证结果对目标化合物具有高度特异性,但与结构相关物质的交叉反应最小。优化条件后,检测限为4.32 ng/mL,定量限为6.84 ~ 107.21 ng/mL。结果表明,所建立的酶联免疫吸附法可用于测定生甘草和市售甘草样品中LicoA的浓度。该试验将有助于甘草及其衍生产品的质量控制,因为它可用于识别植物物种和确定生物活性标记物。
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引用次数: 6
Biotargets for mediation of arsenic–induced coronary heart disease by calycosin 毛蕊异黄酮介导砷诱导冠心病的生物靶点
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-03-31 DOI: 10.1080/09540105.2022.2053947
Hongyuan Xu, Lixiu Qin, Litao Nie, Lin Li, Peng Guo, Yizhao Chen, Chuan Huang, M. Su, Bin Yang
ABSTRACT Arsenic (As), an environmental pollutant, is a highly poisonous metalloid. Accumulated evidence has shown the association between As exposure and elevated risk of the development of coronary heart disease (CHD). Calycosin, a beneficial flavonoid, has demonstrated cardioprotective activities, including those against CHD, in preclinical studies. The anti-As-related CHD activity and mechanism of calycosin have not yet been elucidated. Here, we aimed to determine the core biotargets and molecular mechanisms of calycosin against As-interrelated CHD via integrated bioinformatic analysis, including network pharmacology and molecular docking. The network pharmacology data demonstrated 41 intersection genes of calycosin against As-related CHD, prior to the identification of 15 core targets. Additional in silico investigation indicated that mitogen-activated protein kinase-3 (MAPK3), epidermal growth factor receptor (EGFR), and interleukin-6 (IL6) were the primary pharmacological targets of calycosin for the treatment of As-related CHD. In addition, the therapeutic effects can be realized via cardioprotection-associated signaling pathways for reducing As-induced myocardial toxicity and impairment and boosting CHD functional reparation. In summary, calycosin mediates potent pharmacological effects in As-related CHD therapy functioning via multiple targets and multiple pathways. The results may eventually aid in promoting future clinical application after experimental verification.
砷(As)是一种剧毒类金属,是一种环境污染物。积累的证据表明砷暴露与冠心病(CHD)发病风险升高之间存在关联。毛蕊异黄酮是一种有益的类黄酮,在临床前研究中已证明具有保护心脏的活性,包括抗冠心病的活性。毛蕊异黄酮的抗as相关性冠心病活性及作用机制尚未阐明。本研究旨在通过网络药理学和分子对接等综合生物信息学分析,确定毛蕊异黄酮治疗as相关冠心病的核心生物靶点和分子机制。网络药理学数据显示,毛蕊异黄酮抗as相关性冠心病的交叉基因有41个,鉴定出15个核心靶点。另外,计算机研究表明,丝裂原活化蛋白激酶-3 (MAPK3)、表皮生长因子受体(EGFR)和白细胞介素-6 (IL6)是毛蕊异黄酮治疗as相关性冠心病的主要药理靶点。此外,治疗效果可以通过心脏保护相关的信号通路来实现,减少砷诱导的心肌毒性和损伤,促进冠心病功能修复。综上所述,毛蕊异黄酮通过多靶点和多途径介导了与as相关的冠心病治疗的有效药理作用。经过实验验证,最终有助于促进未来的临床应用。
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引用次数: 0
Aflatoxin B1 variations in animal feeds along the supply chain in Tanzania and its possible reduction by heat treatment 坦桑尼亚供应链动物饲料中黄曲霉毒素B1的变异及其通过热处理可能减少
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-03-04 DOI: 10.1080/09540105.2022.2045908
Anjelina W. Mwakosya, S. Limbu, N. Majaliwa, Xiaobo Zou, Jiyong Shi, O. Kibazohi
ABSTRACT This study evaluated the presence of aflatoxin B1 in five different animal feeds collected from manufacturers, suppliers and consumers and its possible reduction by heating at 100°C for 180 min. A total of 160 animal feed samples were collected and analyzed by using lateral flow immunoassay method. The results revealed that all animal feeds analyzed were positive for aflatoxin B1 with 91% samples containing high concentrations ranging from 24.00 to 76.23 ng/g above the international allowable standard for animal feeds (20 ng/g). Maize bran (76 ng/g) and sunflower cake (63 ng/g) had higher aflatoxin B1 concentrations, correlating with higher moisture content. Upon heating the feeds, aflatoxin B1 was reduced to a concentration ranging from 2.24 to 9.78 ng/g (<20 ng/g). Our study suggests high potential health problems to animals and humans from aflatoxins requiring proper heating and frequent monitoring of the animal feeds for aflatoxin B1.
摘要本研究评估了从制造商、供应商和消费者处收集的五种不同动物饲料中黄曲霉毒素B1的存在,以及在100°C下加热180 min共采集160份动物饲料样品,采用侧流免疫分析法进行分析。结果显示,所有分析的动物饲料都对黄曲霉毒素B1呈阳性,91%的样本含有24.00至76.23的高浓度黄曲霉毒素 ng/g高于动物饲料的国际允许标准(20 ng/g)。玉米糠(76 ng/g)和向日葵蛋糕(63 ng/g)具有较高的黄曲霉毒素B1浓度,与较高的水分含量相关。在加热饲料时,黄曲霉毒素B1的浓度降低到2.24至9.78 纳克/克(<20 ng/g)。我们的研究表明,黄曲霉毒素对动物和人类有很高的潜在健康问题,需要对动物饲料进行适当的加热和频繁的黄曲霉毒素B1监测。
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引用次数: 3
Effects of long-term dietary supplementation of fermented wheat bran on immune performance and inflammatory response in laying hens 长期饲粮中添加发酵麦麸对蛋鸡免疫性能和炎症反应的影响
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-02-21 DOI: 10.1080/09540105.2021.2025346
Yu Wang, Beibei He, Kuanbo Liu, Jingjing Shi, Aike Li, Junlin Cheng, Yuanyuan Wei, Shuangshuang Guo, Yongwei Wang, B. Ding
ABSTRACT This study was conducted to investigate the effects of solid-state fermented wheat bran on immune performance and inflammatory response in laying hens. A total of 225 18-week-old Hy-Line brown-egg laying hens were randomly divided into 3 groups with 5 replicates per group and 15 hens per replicate. Laying hens were fed a basal diet (corn-soybean meal diet) supplemented with 0 (control group), 10% wheat bran and 10% fermented wheat bran, respectively. The results showed: (1) Compared to wheat bran group, the contents of crude protein, trichloroacetic acid-soluble protein, dietary fibre (DF), mannan and total polyphenols in wheat bran were increased by solid state fermentation. (2) Compared to the control group, fermented wheat bran increased the levels of serum biochemical parameters, reproductive hormones, immunoglobulins and anti-inflammatory factors. Therefore, long-term dietary supplementation of 10% fermented wheat bran plays an important role in improving the immune performance of laying hens.
摘要本试验旨在研究固态发酵麦麸对蛋鸡免疫性能和炎症反应的影响。选取18周龄海兰褐蛋蛋鸡225只,随机分为3组,每组5个重复,每个重复15只鸡。蛋鸡饲喂在基础饲粮(玉米-豆粕型饲粮)中分别添加0(对照组)、10%麦麸和10%发酵麦麸的试验饲粮。结果表明:(1)与麦麸组相比,固态发酵提高了麦麸中粗蛋白质、三氯乙酸可溶性蛋白、膳食纤维、甘露聚糖和总多酚含量。(2)与对照组相比,发酵麦麸提高了血清生化指标、生殖激素、免疫球蛋白和抗炎因子水平。因此,长期饲粮中添加10%发酵麦麸对提高蛋鸡免疫性能具有重要作用。
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引用次数: 4
The effect of the multi-strain probiotic preparation EM Bokashi® on selected parameters of the cellular immune response in pigs 多菌种益生菌制剂EM博卡希®对猪细胞免疫应答部分参数的影响
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-02-17 DOI: 10.1080/09540105.2021.2006611
Ł. Jarosz, A. Ciszewski, A. Marek, M. Hejdysz, S. Nowaczewski, Z. Grądzki, K. Michalak, M. Kwiecień, A. Rysiak
ABSTRACT The aim of the study was to assess the effect of effective microorganisms on selected parameters of the cellular immune response in the colostrum and milk of sows and in the serum of suckling piglets. A total of 60 female crossbred sows and 60 female piglets were used in the experiment. The material for analysis comprised colostrum and milk collected from sows and blood samples from piglets. Immune response parameters were determined by flow cytometry and ELISA kits. The results showed high expression of CD4-CD25-FoxP3, CD4, and CD25 in the colostrum and milk of sows and of CD4, CD8 in piglet blood. The use of EM Bokashi® as a feed supplement for sows during colostrogenesis stimulates cellular immune mechanisms, expressed by an increase in the Treg cell population in the colostrum and milk, which contributes to effective elimination of microorganisms in the first period of piglets’ life.
摘要本研究的目的是评估有效微生物对母猪初乳、乳汁和乳猪血清中细胞免疫反应选定参数的影响。试验选用60头杂交母猪和60头仔猪。用于分析的材料包括从母猪身上采集的初乳和牛奶以及从仔猪身上提取的血液样本。通过流式细胞术和ELISA试剂盒测定免疫反应参数。结果显示,CD4-CD25-FoxP3、CD4和CD25在母猪的初乳和乳汁中高表达,CD4、CD8在仔猪血液中高表达。EM Bokashi®作为母猪在造瘘过程中的饲料补充剂,可以刺激细胞免疫机制,通过增加初乳和乳汁中的Treg细胞群来表达,这有助于在仔猪生命的第一阶段有效消除微生物。
{"title":"The effect of the multi-strain probiotic preparation EM Bokashi® on selected parameters of the cellular immune response in pigs","authors":"Ł. Jarosz, A. Ciszewski, A. Marek, M. Hejdysz, S. Nowaczewski, Z. Grądzki, K. Michalak, M. Kwiecień, A. Rysiak","doi":"10.1080/09540105.2021.2006611","DOIUrl":"https://doi.org/10.1080/09540105.2021.2006611","url":null,"abstract":"ABSTRACT The aim of the study was to assess the effect of effective microorganisms on selected parameters of the cellular immune response in the colostrum and milk of sows and in the serum of suckling piglets. A total of 60 female crossbred sows and 60 female piglets were used in the experiment. The material for analysis comprised colostrum and milk collected from sows and blood samples from piglets. Immune response parameters were determined by flow cytometry and ELISA kits. The results showed high expression of CD4-CD25-FoxP3, CD4, and CD25 in the colostrum and milk of sows and of CD4, CD8 in piglet blood. The use of EM Bokashi® as a feed supplement for sows during colostrogenesis stimulates cellular immune mechanisms, expressed by an increase in the Treg cell population in the colostrum and milk, which contributes to effective elimination of microorganisms in the first period of piglets’ life.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46351898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monoclonal antibodies against the newly identified allergen β-enolase from common carp (Cyprinus carpio) 鲤鱼过敏原β-烯醇化酶单克隆抗体
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-02-14 DOI: 10.1080/09540105.2022.2028741
Aistė Sližienė, M. Pleckaityte, Mindaugas Zaveckas, K. Juškaitė, Vytautas Rudokas, G. Žvirblis, A. Zvirbliene
ABSTRACT β-enolase is a heat-labile fish allergen identified in different fish species. In this study, β-enolase gene was cloned from common carp muscle tissue and the target protein was expressed as a fusion with maltose binding protein (MBP-Eno) in E. coli. Recombinant MBP-Eno was reactive with blood serum specimens of fish-allergic patients, confirming that β-enolase is a newly identified allergen of common carp. Two monoclonal antibodies (MAbs) against recombinant β-enolase were generated that showed cross-reactivity with β-enolases of other organisms. Both antibodies recognized β-enolases from other fish species extracts, while MAb 6E4 showed a broad reactivity with pork, chicken, yeast, and E. coli samples. Epitope mapping using MBP-Eno variants revealed that β-enolase region comprising amino acids 623–698 presumably includes MAb 6E4 epitope. The generated broadly reactive MAb 6E4 directed against a conserved epitope of β-enolases may represent a valuable tool for the characterization of allergen extracts and fish allergy diagnostics.
摘要β-烯醇化酶是一种在不同鱼类中发现的对热不稳定的鱼类过敏原。本研究从鲤鱼肌肉组织中克隆了β-烯醇化酶基因,并将其与麦芽糖结合蛋白(MBP-Eno)融合表达于大肠杆菌中。重组MBP-Eno与鱼类过敏患者的血清标本反应,证实β-烯醇化酶是一种新发现的常见鲤鱼过敏原。产生了两种针对重组β-烯醇化酶的单克隆抗体(MAbs),它们与其他生物体的β-烯醇酶表现出交叉反应性。两种抗体都能识别其他鱼类提取物中的β-烯醇化酶,而MAb6E4对猪肉、鸡肉、酵母和大肠杆菌样品表现出广泛的反应性。使用MBP-Eno变体的表位定位显示,包含氨基酸623-698的β-烯醇化酶区域可能包括MAb6E4表位。所产生的针对β-烯醇化酶保守表位的广泛反应性MAb6E4可能是表征过敏原提取物和鱼类过敏诊断的有价值的工具。
{"title":"Monoclonal antibodies against the newly identified allergen β-enolase from common carp (Cyprinus carpio)","authors":"Aistė Sližienė, M. Pleckaityte, Mindaugas Zaveckas, K. Juškaitė, Vytautas Rudokas, G. Žvirblis, A. Zvirbliene","doi":"10.1080/09540105.2022.2028741","DOIUrl":"https://doi.org/10.1080/09540105.2022.2028741","url":null,"abstract":"ABSTRACT β-enolase is a heat-labile fish allergen identified in different fish species. In this study, β-enolase gene was cloned from common carp muscle tissue and the target protein was expressed as a fusion with maltose binding protein (MBP-Eno) in E. coli. Recombinant MBP-Eno was reactive with blood serum specimens of fish-allergic patients, confirming that β-enolase is a newly identified allergen of common carp. Two monoclonal antibodies (MAbs) against recombinant β-enolase were generated that showed cross-reactivity with β-enolases of other organisms. Both antibodies recognized β-enolases from other fish species extracts, while MAb 6E4 showed a broad reactivity with pork, chicken, yeast, and E. coli samples. Epitope mapping using MBP-Eno variants revealed that β-enolase region comprising amino acids 623–698 presumably includes MAb 6E4 epitope. The generated broadly reactive MAb 6E4 directed against a conserved epitope of β-enolases may represent a valuable tool for the characterization of allergen extracts and fish allergy diagnostics.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42599947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Kadsura japonica fruits exert immunostimulatory and anti-obesity activity in RAW264.7 and 3T3-L1 cells 桂皮对RAW264.7和3T3-L1细胞具有免疫刺激和抗肥胖活性
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-02-04 DOI: 10.1080/09540105.2021.2024151
Na Gyeong Geum, Ho-Jun Son, Julia Yu, J. Yeo, Min Yeong Choi, Jae Won Lee, J. Baek, H. Eo, G. Park, J. Jeong
ABSTRACT Under the COVID-19 pandemic, interest in immune enhancement and anti-obesity is increasing. Thus, in this study, we investigated whether Kadsura japonica fruits (KJF) exhibits immunostimulatory activity and anti-obesity activity. KJF increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. Inhibition of TLR2 and TLR4 blocked KJF-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of MAPK and PI3 K/AKT signaling pathway reduced KJF-mediated production of immunostimulatory factors, and the activation of MAPK and PI3 K/AKT signaling pathway by KJF suppressed the inhibition of TLR2/4. KJF attenuated the lipid accumulation and the protein expression such as CEBPα, PPARγ, perilipin-1, adiponectin, and FABP4 related to the lipid accumulation in 3T3-L1 cells. In addition, KJF inhibited excessive proliferation of 3T3-L1 cells and protein expressions such as β-catenin and cyclin D1 related to cell growth. These findings indicate that KJF may have immunostimulatory activity and anti-obesity activity.
摘要在新冠肺炎大流行的背景下,人们对增强免疫和抗肥胖的兴趣正在增加。因此,在本研究中,我们研究了南五味子(KJF)是否具有免疫刺激活性和抗肥胖活性。KJF增加了RAW264.7细胞中免疫刺激因子的产生和吞噬作用。TLR2和TLR4的抑制阻断了KJF介导的RAW264.7细胞中免疫刺激因子的产生。此外,MAPK和PI3K/AKT信号通路的抑制降低了KJF介导的免疫刺激因子的产生,而KJF对MAPK和pi3K/AKT信号通路的激活抑制了TLR2/4的抑制。KJF减弱了3T3-L1细胞中的脂质积聚和与脂质积聚相关的蛋白质表达,如CEBPα、PPARγ、周脂素-1、脂联素和FABP4。此外,KJF抑制3T3-L1细胞的过度增殖以及与细胞生长相关的蛋白表达,如β-连环蛋白和细胞周期蛋白D1。这些发现表明KJF可能具有免疫刺激活性和抗肥胖活性。
{"title":"Kadsura japonica fruits exert immunostimulatory and anti-obesity activity in RAW264.7 and 3T3-L1 cells","authors":"Na Gyeong Geum, Ho-Jun Son, Julia Yu, J. Yeo, Min Yeong Choi, Jae Won Lee, J. Baek, H. Eo, G. Park, J. Jeong","doi":"10.1080/09540105.2021.2024151","DOIUrl":"https://doi.org/10.1080/09540105.2021.2024151","url":null,"abstract":"ABSTRACT Under the COVID-19 pandemic, interest in immune enhancement and anti-obesity is increasing. Thus, in this study, we investigated whether Kadsura japonica fruits (KJF) exhibits immunostimulatory activity and anti-obesity activity. KJF increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. Inhibition of TLR2 and TLR4 blocked KJF-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of MAPK and PI3 K/AKT signaling pathway reduced KJF-mediated production of immunostimulatory factors, and the activation of MAPK and PI3 K/AKT signaling pathway by KJF suppressed the inhibition of TLR2/4. KJF attenuated the lipid accumulation and the protein expression such as CEBPα, PPARγ, perilipin-1, adiponectin, and FABP4 related to the lipid accumulation in 3T3-L1 cells. In addition, KJF inhibited excessive proliferation of 3T3-L1 cells and protein expressions such as β-catenin and cyclin D1 related to cell growth. These findings indicate that KJF may have immunostimulatory activity and anti-obesity activity.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42754091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Development of a specific monoclonal antibody-based icELISA for detection of arecoline in traditional Chinese medicines and fresh areca nuts 基于单克隆抗体的icELISA检测中药和新鲜槟榔果中槟榔碱的建立
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-02-04 DOI: 10.1080/09540105.2021.2025347
Yunhe Wang, Mengying Ding, Hudong Ma, Jiao Wu, Hongwei Zhao, Yinglang Wan
ABSTRACT Arecoline, the dominant alkaloid existing in areca nuts, is an addictive substance and classified as a Group 2B potential human carcinogen. Currently, the detection of arecoline is mostly dependent on chromatography-based approaches, which are time-consuming and expensive. We used arecaidine as a hapten to produce a highly specific monoclonal antibody (mAb) against arecoline. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed using the mAb-A5H12, to detect arecoline in traditional Chinese medicines and fresh areca nuts. The icELISA indicated that the half maximum inhibition concentration (IC50) for arecoline was 67.9 ng/mL, with a working range of 10.1–502.6 ng/mL and a limit of detection (LOD) of 3.6 ng/mL. High-performance liquid chromatographic (HPLC) confirmed the accuracy and the working range of icELISA, suggesting that the icELISA approach based on the arecoline specific antibody could be a widely applicable and easy operation method in detection of arecoline in foods and medicines.
槟榔碱是存在于槟榔果中的主要生物碱,是一种成瘾性物质,被列为2B类潜在人类致癌物。目前,槟榔碱的检测主要依赖于基于色谱的方法,这种方法耗时且昂贵。我们用槟榔碱作为半抗原制备了一种高特异性的槟榔碱单克隆抗体(mAb)。采用单克隆抗体- a5h12建立了一种间接竞争酶联免疫吸附试验(icELISA),用于检测中药和新鲜槟榔果中的槟榔碱。icELISA结果表明,槟榔碱的半数最大抑制浓度(IC50)为67.9 ng/mL,工作范围为10.1 ~ 502.6 ng/mL,检出限(LOD)为3.6 ng/mL。高效液相色谱(HPLC)证实了icELISA方法的准确性和工作范围,提示基于槟榔碱特异性抗体的icELISA方法在食品和药品中槟榔碱的检测中具有广泛的适用性和简便的操作方法。
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引用次数: 4
Luteolin attenuates PM2.5-induced inflammatory responses by augmenting HO-1 and JAK-STAT expression in murine alveolar macrophages 木犀草素通过增加小鼠肺泡巨噬细胞HO-1和JAK-STAT的表达来减弱pm2.5诱导的炎症反应
IF 3 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2022-02-04 DOI: 10.1080/09540105.2021.2022605
Wen-Che Hsieh, C. Lai, H. Lin, D. Tu, T. Shen, Yi-Ju Lee, M. Hsieh, Ching-Chung Chen, Hsin-Hsuan Han, Yuan-Yen Chang
ABSTRACT To explore the involved mechanisms and possible treatments of ambient PM2.5 exposure-induced lung inflammation, this work studied the activity of luteolin, a natural flavonoid which widely presents in many plant species, in murine alveolar macrophage MH S cells exposed to PM2.5. Results showed PM2.5 induced an inflammatory response, as evidenced by significantly increased TNF-α, IL-6, MCP-1 and Rantes levels. and induced iNOS, COX-2, and NF-κB protein expressions in MH-S cells. Moreover, luteolin pre-treatment reduced JAK2 and STAT1 but not STAT3 protein expressions in PM2.5-stimulated MH-S cells. Performing JAK2 inhibitor AG490 further showed reduced TNF-α and IL-6 productions as well as iNOS, COX-2, and NF-κB protein expressions. In addition, although PM2.5 exposure could elevate HO-1 expression basically, luteolin pre-treatment and AG490 administration further significantly enhanced HO-1 expression additionally. Collectively, these results revealed that luteolin inhibits inflammation through suppressing JAK2/STAT1/NF-κB pathway and enhancing HO-1 expression in PM2.5-challenged alveolar macrophage MH-S cells.
摘要为了探讨环境PM2.5暴露诱导肺部炎症的相关机制和可能的治疗方法,本工作研究了木犀草素(一种广泛存在于多种植物中的天然类黄酮)在暴露于PM2.5的小鼠肺泡巨噬细胞MHS细胞中的活性。结果显示,PM2.5诱导了炎症反应,TNF-α、IL-6、MCP-1和Rantes水平显著升高。并诱导MH-S细胞中iNOS、COX-2和NF-κB蛋白的表达。此外,木犀草素预处理降低了PM2.5刺激的MH-S细胞中JAK2和STAT1蛋白的表达,但没有降低STAT3蛋白的表达。执行JAK2抑制剂AG490进一步显示TNF-α和IL-6的产生以及iNOS、COX-2和NF-κB蛋白的表达减少。此外,尽管PM2.5暴露基本上可以提高HO-1的表达,但木犀草素预处理和AG490给药进一步显著提高了HO-1的表现。总之,这些结果表明木犀草素通过抑制JAK2/STAT1/NF-κB通路和增强PM2.5激发的肺泡巨噬细胞MH-S细胞中HO-1的表达来抑制炎症。
{"title":"Luteolin attenuates PM2.5-induced inflammatory responses by augmenting HO-1 and JAK-STAT expression in murine alveolar macrophages","authors":"Wen-Che Hsieh, C. Lai, H. Lin, D. Tu, T. Shen, Yi-Ju Lee, M. Hsieh, Ching-Chung Chen, Hsin-Hsuan Han, Yuan-Yen Chang","doi":"10.1080/09540105.2021.2022605","DOIUrl":"https://doi.org/10.1080/09540105.2021.2022605","url":null,"abstract":"ABSTRACT\u0000 To explore the involved mechanisms and possible treatments of ambient PM2.5 exposure-induced lung inflammation, this work studied the activity of luteolin, a natural flavonoid which widely presents in many plant species, in murine alveolar macrophage MH S cells exposed to PM2.5. Results showed PM2.5 induced an inflammatory response, as evidenced by significantly increased TNF-α, IL-6, MCP-1 and Rantes levels. and induced iNOS, COX-2, and NF-κB protein expressions in MH-S cells. Moreover, luteolin pre-treatment reduced JAK2 and STAT1 but not STAT3 protein expressions in PM2.5-stimulated MH-S cells. Performing JAK2 inhibitor AG490 further showed reduced TNF-α and IL-6 productions as well as iNOS, COX-2, and NF-κB protein expressions. In addition, although PM2.5 exposure could elevate HO-1 expression basically, luteolin pre-treatment and AG490 administration further significantly enhanced HO-1 expression additionally. Collectively, these results revealed that luteolin inhibits inflammation through suppressing JAK2/STAT1/NF-κB pathway and enhancing HO-1 expression in PM2.5-challenged alveolar macrophage MH-S cells.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47796852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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Food and Agricultural Immunology
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