Pub Date : 2022-12-15DOI: 10.1080/09540105.2022.2133090
Adam Wawrzenczyk, Emilia Rawicka, Katarzyna Napiórkowska-Baran, Ewa Alska, Z. Bartuzi
ABSTRACT The aim of this study was to identify allergenic cross-reacting allergens in patients with inhalation and food allergy using molecular diagnostics ISAC test. The study group consisted of 50 adult patients. The control group contained 20 healthy people. In the study group 92% patients were found to have the asIgE component responsible for cross-allergy between plant pollens and foods. The presence of asIgE was the most common for the Bet v 1 component. AsIgE was also detected for the component responsible for the presence of a true allergy to 26% patients. A statistically significant frequency of sensitization to 12 cross-reactive components was demonstrated, 10 of them belonged to the PR-10 proteins. An allergy profile of the studied population was created based on the ISAC results. The results of the study suggest that cross-reactions are the main cause of food allergy, and their frequency rises with increased sensitization to inhaled allergens.
{"title":"Cross-reactive aeroallergens – the main cause of food allergy","authors":"Adam Wawrzenczyk, Emilia Rawicka, Katarzyna Napiórkowska-Baran, Ewa Alska, Z. Bartuzi","doi":"10.1080/09540105.2022.2133090","DOIUrl":"https://doi.org/10.1080/09540105.2022.2133090","url":null,"abstract":"ABSTRACT The aim of this study was to identify allergenic cross-reacting allergens in patients with inhalation and food allergy using molecular diagnostics ISAC test. The study group consisted of 50 adult patients. The control group contained 20 healthy people. In the study group 92% patients were found to have the asIgE component responsible for cross-allergy between plant pollens and foods. The presence of asIgE was the most common for the Bet v 1 component. AsIgE was also detected for the component responsible for the presence of a true allergy to 26% patients. A statistically significant frequency of sensitization to 12 cross-reactive components was demonstrated, 10 of them belonged to the PR-10 proteins. An allergy profile of the studied population was created based on the ISAC results. The results of the study suggest that cross-reactions are the main cause of food allergy, and their frequency rises with increased sensitization to inhaled allergens.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"1 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41573684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-17DOI: 10.1080/09540105.2022.2144145
Peizhen Li, Xiuhua Xia, Jiannan Chen, Hang Yu, Yunfei Xie, Yahui Guo, Weirong Yao, H. Qian, Yuliang Cheng
ABSTRACT Gold-coated silver core–shell nanoparticles (Ag@Au NPs) have been widely used for SERS analysis due to plasmon resonance performances. However, it is a great challenge to fabricate Ag@Au core–shell structures with specified morphology. In this paper, the size and shape of Ag NPs (core) were precisely controlled by a seed-mediated approach, and then the gold shell was completely coated on under the strong reduction of ascorbic acid, resulting in Ag@Au NPs with highly regulated morphology. The obtained Ag@Au NPs achieved a similar or even better SERS enhancement factor (EF) (up to 5.7 × 107) compared to Ag NPs, but significantly higher stability. For the rapid SERS detection of nitrofurantoin metabolite AHD, a limit of detection (LOD) as low as 1 × 10−10 M and a wide linear range from 1 × 10−4 to 1 × 10−8 M were obtained. Furthermore, recoveries for crayfish samples were from 90.5% to 108%, indicating a good performance of the presented method in real sample detection.
{"title":"Morphology-regulated core–shell Ag@Au NPs for rapid SERS detection of 1-amino-hydantoin (AHD) in crayfish","authors":"Peizhen Li, Xiuhua Xia, Jiannan Chen, Hang Yu, Yunfei Xie, Yahui Guo, Weirong Yao, H. Qian, Yuliang Cheng","doi":"10.1080/09540105.2022.2144145","DOIUrl":"https://doi.org/10.1080/09540105.2022.2144145","url":null,"abstract":"ABSTRACT Gold-coated silver core–shell nanoparticles (Ag@Au NPs) have been widely used for SERS analysis due to plasmon resonance performances. However, it is a great challenge to fabricate Ag@Au core–shell structures with specified morphology. In this paper, the size and shape of Ag NPs (core) were precisely controlled by a seed-mediated approach, and then the gold shell was completely coated on under the strong reduction of ascorbic acid, resulting in Ag@Au NPs with highly regulated morphology. The obtained Ag@Au NPs achieved a similar or even better SERS enhancement factor (EF) (up to 5.7 × 107) compared to Ag NPs, but significantly higher stability. For the rapid SERS detection of nitrofurantoin metabolite AHD, a limit of detection (LOD) as low as 1 × 10−10 M and a wide linear range from 1 × 10−4 to 1 × 10−8 M were obtained. Furthermore, recoveries for crayfish samples were from 90.5% to 108%, indicating a good performance of the presented method in real sample detection.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"832 - 847"},"PeriodicalIF":3.0,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49101721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-30DOI: 10.1080/09540105.2022.2137472
Xiangxue Ning, Jing Qiu
ABSTRACT Amantadine is one of the most prescribed antiviral medications. Considering its abuse is life-threatening, monitoring amantadine residues is urgently needed. This study designed a dipstick assay based on lateral flow immunoassay (LFIA) platforms with an indicator range of 50–200 ng/mL. The dipstick consists of four coloured test lines that detect the concentration of amantadine semi-quantitatively in the range of 0–200 ng/mL. The amantadine concentration was categorised as 0–50, 50–100, 100–200, or > 200 ng/mL, depending on the response of the test lines. Furthermore, we measured the amantadine residues in commercial meat samples by this LFIA. The results were highly agreeable with those determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LFIA shows potential for routine monitoring of amantadine in meat products, and the results of this study provide insight into the development of new LFIA devices for use in agricultural industries.
{"title":"A semi-quantitative multi-range lateral flow immunoassay for amantadine residues in livestock and poultry products","authors":"Xiangxue Ning, Jing Qiu","doi":"10.1080/09540105.2022.2137472","DOIUrl":"https://doi.org/10.1080/09540105.2022.2137472","url":null,"abstract":"ABSTRACT Amantadine is one of the most prescribed antiviral medications. Considering its abuse is life-threatening, monitoring amantadine residues is urgently needed. This study designed a dipstick assay based on lateral flow immunoassay (LFIA) platforms with an indicator range of 50–200 ng/mL. The dipstick consists of four coloured test lines that detect the concentration of amantadine semi-quantitatively in the range of 0–200 ng/mL. The amantadine concentration was categorised as 0–50, 50–100, 100–200, or > 200 ng/mL, depending on the response of the test lines. Furthermore, we measured the amantadine residues in commercial meat samples by this LFIA. The results were highly agreeable with those determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LFIA shows potential for routine monitoring of amantadine in meat products, and the results of this study provide insight into the development of new LFIA devices for use in agricultural industries.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"817 - 831"},"PeriodicalIF":3.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47508030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-20DOI: 10.1080/09540105.2022.2134313
Jiaxin Chen, Manqing Sun, Xinmu Cui, Xuewu Zhang
ABSTRACT Compound K (CK) is the metabolite and final active ingredient of diol-type ginsenosides. In this study, we investigated the effect of CK on mitochondrial apoptosis in SMMC-7721 and BEL-7404 cells and the regulatory mechanism through in vitro and in vivo experiments. The results demonstrated that CK inhibited Hepatocellular carcinoma (HCC) cells proliferation and arrested the cells in G0/G1 phase. CK induces mitochondrial apoptosis in HCC cells and inhibited p-ERK expression. Bcl-2 associated transcription factor 1 (Bclaf1) was distributed in the nucleus and cytoplasm, and CK inhibited its expression. Treatment of a nude mouse xenograft model bearing SMMC-7721 cells with CK decreased the expression of Bclaf1, p-ERK, and Bcl-2 but increased that of Bax. In summary, ginsenoside CK downregulated Bclaf1 expression, inhibited the activation of the ERK pathway, and triggered mitochondrial apoptosis in HCC. These findings uncovered a potential therapeutic strategy leveraging the anti-tumor effects of CK against HCC.
{"title":"Ginsenoside compound K induces mitochondrial apoptosis in human hepatoma cells through Bclaf1-mediated modulation of ERK signaling","authors":"Jiaxin Chen, Manqing Sun, Xinmu Cui, Xuewu Zhang","doi":"10.1080/09540105.2022.2134313","DOIUrl":"https://doi.org/10.1080/09540105.2022.2134313","url":null,"abstract":"ABSTRACT Compound K (CK) is the metabolite and final active ingredient of diol-type ginsenosides. In this study, we investigated the effect of CK on mitochondrial apoptosis in SMMC-7721 and BEL-7404 cells and the regulatory mechanism through in vitro and in vivo experiments. The results demonstrated that CK inhibited Hepatocellular carcinoma (HCC) cells proliferation and arrested the cells in G0/G1 phase. CK induces mitochondrial apoptosis in HCC cells and inhibited p-ERK expression. Bcl-2 associated transcription factor 1 (Bclaf1) was distributed in the nucleus and cytoplasm, and CK inhibited its expression. Treatment of a nude mouse xenograft model bearing SMMC-7721 cells with CK decreased the expression of Bclaf1, p-ERK, and Bcl-2 but increased that of Bax. In summary, ginsenoside CK downregulated Bclaf1 expression, inhibited the activation of the ERK pathway, and triggered mitochondrial apoptosis in HCC. These findings uncovered a potential therapeutic strategy leveraging the anti-tumor effects of CK against HCC.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"799 - 816"},"PeriodicalIF":3.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43996347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-20DOI: 10.1080/09540105.2022.2130183
J. Čelakovská, R. Vankova, H. Skalská, J. Krejsek, C. Andrys
ABSTRACT This study aims to analyse the results of specific IgE to molecular components of PR 10 proteins, mould and yeast in atopic dermatitis (AD) patients, to find significant sets of molecular components in pairs and triplets in the relation to the severity of atopic dermatitis and the occurrence of asthma bronchiale and allergic rhinitis. The examination of the sensitization to molecular components with ALEX2 Allergy Explorer testing was performed. The level of specific IgE was recorded with Jittered box plots, the Kruskal–Wallis test was used to compare the level of specific IgE in different forms of AD. The relation between the results of specific IgE was evaluated with Coefficient of correlation (Kendall tau-b). The comparison of significant rules was performed with Fisher–Freeman–Halton exact test and with tests on proportions. Altogether 100 atopic dermatitis patients were examined. Molecular components Fra a 1 + 3, Mal d 1, Api g 1, Mala s 11 and Asp f 6 are recorded as a single molecular component with relation to the severe form of AD; these components are associated in pairs and triplets with molecular components Cor a 1.0401, Cor a 1.0103, Fag s 1, Alt a 1 and with the occurrence of allergic rhinitis. Results are presented in static and interactive tables and plots.
摘要本研究旨在分析特应性皮炎（AD）患者PR 10蛋白、霉菌和酵母分子组分的特异性IgE结果，以发现与特应性皮肤炎的严重程度、哮喘支气管哮喘和过敏性鼻炎的发生有关的成对和三重组分的重要分子组分。用ALEX2 Allergy Explorer测试对分子成分的致敏性进行检查。特异性IgE水平用吉布盒图记录，Kruskal–Wallis检验用于比较不同形式AD中的特异性IgE水平。用相关系数（Kendall tau-b）评估特异性IgE.结果之间的关系。显著规则的比较采用Fisher–Freeman–Halton精确检验和比例检验。共检查了100名特应性皮炎患者。分子组分Fra a 1 + 3、Mal d1、Api g1、Mala s11和Asp f6被记录为与严重形式的AD有关的单一分子组分；这些组分与分子组分Cor a 1.0401、Cor a 1.0103、Fag s 1、Alt a 1成对和三联体相关，并与过敏性鼻炎的发生相关。结果显示在静态和交互式表格和图表中。
{"title":"The role of PR 10 proteins and molecular components of moulds and yeast in atopic dermatitis patients","authors":"J. Čelakovská, R. Vankova, H. Skalská, J. Krejsek, C. Andrys","doi":"10.1080/09540105.2022.2130183","DOIUrl":"https://doi.org/10.1080/09540105.2022.2130183","url":null,"abstract":"ABSTRACT This study aims to analyse the results of specific IgE to molecular components of PR 10 proteins, mould and yeast in atopic dermatitis (AD) patients, to find significant sets of molecular components in pairs and triplets in the relation to the severity of atopic dermatitis and the occurrence of asthma bronchiale and allergic rhinitis. The examination of the sensitization to molecular components with ALEX2 Allergy Explorer testing was performed. The level of specific IgE was recorded with Jittered box plots, the Kruskal–Wallis test was used to compare the level of specific IgE in different forms of AD. The relation between the results of specific IgE was evaluated with Coefficient of correlation (Kendall tau-b). The comparison of significant rules was performed with Fisher–Freeman–Halton exact test and with tests on proportions. Altogether 100 atopic dermatitis patients were examined. Molecular components Fra a 1 + 3, Mal d 1, Api g 1, Mala s 11 and Asp f 6 are recorded as a single molecular component with relation to the severe form of AD; these components are associated in pairs and triplets with molecular components Cor a 1.0401, Cor a 1.0103, Fag s 1, Alt a 1 and with the occurrence of allergic rhinitis. Results are presented in static and interactive tables and plots.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"780 - 798"},"PeriodicalIF":3.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44073610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-10DOI: 10.1080/09540105.2022.2128070
Liu Song-xin, Li Zhi-man, Sha Zi-Jun, Xia Yun-shi, Zhao Li-juan, Ren Duo-duo, Sun Yin-shi
ABSTRACT To study the antioxidant and immune protection activity of velvet antler (VA) in cyclophosphamide-induced immunocompromised mice, BALB/c mice were intragastrically administered 100, 300, or 500 mg/(kg·d) VA for 30 days, and were intraabdominally injected with cyclophosphamide to create a hypoimmune murine model. Cellular immunity, macrophage phagocytic activity, natural killer (NK) cell activity, white blood cell count, splenic lymphocyte subsets and the mRNA expression levels of serum cytokines were analysed. We found that the IC50 for removing 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), OH, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals of VA were 0.476, 4.33, and 37.92 mg/mL, respectively. VA could enhance innate and adaptive immunity of mice by improving cellular immunity, macrophage phagocytic ability, and NK cell activity; promoting the production of IL-4, IFN-γ, TNF-α and other cytokines; increasing the content and ratio of lymphocyte subsets. These results indicate that VA has good antioxidant activity and can improve the immune function of cyclophosphamide-induced immunodeficient mice.
{"title":"Effect of velvet antler on the immune activity of cyclophosphamide-induced immunosuppressed mice","authors":"Liu Song-xin, Li Zhi-man, Sha Zi-Jun, Xia Yun-shi, Zhao Li-juan, Ren Duo-duo, Sun Yin-shi","doi":"10.1080/09540105.2022.2128070","DOIUrl":"https://doi.org/10.1080/09540105.2022.2128070","url":null,"abstract":"ABSTRACT To study the antioxidant and immune protection activity of velvet antler (VA) in cyclophosphamide-induced immunocompromised mice, BALB/c mice were intragastrically administered 100, 300, or 500 mg/(kg·d) VA for 30 days, and were intraabdominally injected with cyclophosphamide to create a hypoimmune murine model. Cellular immunity, macrophage phagocytic activity, natural killer (NK) cell activity, white blood cell count, splenic lymphocyte subsets and the mRNA expression levels of serum cytokines were analysed. We found that the IC50 for removing 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), OH, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals of VA were 0.476, 4.33, and 37.92 mg/mL, respectively. VA could enhance innate and adaptive immunity of mice by improving cellular immunity, macrophage phagocytic ability, and NK cell activity; promoting the production of IL-4, IFN-γ, TNF-α and other cytokines; increasing the content and ratio of lymphocyte subsets. These results indicate that VA has good antioxidant activity and can improve the immune function of cyclophosphamide-induced immunodeficient mice.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"768 - 779"},"PeriodicalIF":3.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45431297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive and quantitative detection of florfenicol (FF) in food samples was developed. Au nanocubers (AuNCs) with long wavelength absorption were synthesised, characterised and used as the substrate in SERS-ICA. Immunoprobe was prepared by linking Raman reporter and the antibody against FF on AuNCs. The SERS-ICA was completed in 15 min. By measuring the Raman intensities on T lines, quantitative detection of FF was achieved. The IC50 and limit of detection (LOD) of the assay for FF were 0.027 ng mL−1 and 0.12 pg mL−1, respectively. The cross-reactivity (CR) of the assay with thiamphenicol, chloramphenicol and other four drugs were examined. The recoveries of FF in spiked samples were 88.5% ∼ 102.4% with RSD of 2.3% ∼ 7.4%. It was proven that SERS-ICA was able to rapidly detect FF in food samples with high sensitivity, accuracy and precision.
摘要建立了一种基于表面增强拉曼散射（SERS）的免疫色谱法（ICA），用于食品样品中氟苯尼考（FF）的超灵敏定量检测。合成了具有长波长吸收的Au纳米立方体（AuNCs），对其进行了表征，并将其用作SERS-ICA的基底。通过在AuNCs上连接拉曼报告子和针对FF的抗体来制备免疫探针。SERS-ICA于15年完成 min。通过测量T线上的拉曼强度，实现了FF的定量检测。FF测定的IC50和检测限（LOD）为0.027 ng mL−1和0.12 pg mL−1。检测了该方法与氯霉素、硫酚等4种药物的交叉反应性（CR）。加标样品中FF的回收率为88.5%～102.4%，RSD为2.3%～7.4%。SERS-ICA能够快速检测食品样品中的FF，具有较高的灵敏度、准确性和精密度。
{"title":"A SERS-based immunochromatographic assay for ultrasensitive and quantitative detection of florfenicol using long wavelength absorption of Au nanocubes","authors":"Ying Liu, Ting Zhou, Penghui Zhou, Hanwen Liu, Jianguo Li, Anping Deng","doi":"10.1080/09540105.2022.2120852","DOIUrl":"https://doi.org/10.1080/09540105.2022.2120852","url":null,"abstract":"ABSTRACT An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive and quantitative detection of florfenicol (FF) in food samples was developed. Au nanocubers (AuNCs) with long wavelength absorption were synthesised, characterised and used as the substrate in SERS-ICA. Immunoprobe was prepared by linking Raman reporter and the antibody against FF on AuNCs. The SERS-ICA was completed in 15 min. By measuring the Raman intensities on T lines, quantitative detection of FF was achieved. The IC50 and limit of detection (LOD) of the assay for FF were 0.027 ng mL−1 and 0.12 pg mL−1, respectively. The cross-reactivity (CR) of the assay with thiamphenicol, chloramphenicol and other four drugs were examined. The recoveries of FF in spiked samples were 88.5% ∼ 102.4% with RSD of 2.3% ∼ 7.4%. It was proven that SERS-ICA was able to rapidly detect FF in food samples with high sensitivity, accuracy and precision.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"752 - 767"},"PeriodicalIF":3.0,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43131208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1080/09540105.2022.2120851
F. Liu, Chien-Li Chen, Chung-Hsiung Huang
ABSTRACT A growing number of people choose plant-based milk products to replace dairy for health reasons or veganism. Since fermentation is a promising method to improve the nutritional value of plant-based milk, this study aimed to establish the process of oat milk fermentation and to evaluate the effect of fermented oat milk (FOM) on modulating antigen-specific immune response in ovalbumin (OVA)-sensitized mice. The FOM was prepared by fermentation of oat milk with Streptococcus thermophilus, Lactobacillus bulgaricus and proteolytic L. rhamnosus. Addition of 1% glucose improved the process of fermentation. FOM was stable while stored at 4°C within seven days. Oral administration with FOM (0.4 and 2 g/kg body weight) did not alter the serum level of OVA-specific IgG and spleen index. However, FOM had comparable potency with commercial dairy yogurt to lower stimulation index, IL-2, IFN-γ and IL-4 production and to promote TGF-β and IL-10 secretion by OVA-stimulated splenocytes.
{"title":"Preparation of fermented oat milk and evaluation of its modulatory effect on antigen-specific immune responses in ovalbumin-sensitized mice","authors":"F. Liu, Chien-Li Chen, Chung-Hsiung Huang","doi":"10.1080/09540105.2022.2120851","DOIUrl":"https://doi.org/10.1080/09540105.2022.2120851","url":null,"abstract":"ABSTRACT A growing number of people choose plant-based milk products to replace dairy for health reasons or veganism. Since fermentation is a promising method to improve the nutritional value of plant-based milk, this study aimed to establish the process of oat milk fermentation and to evaluate the effect of fermented oat milk (FOM) on modulating antigen-specific immune response in ovalbumin (OVA)-sensitized mice. The FOM was prepared by fermentation of oat milk with Streptococcus thermophilus, Lactobacillus bulgaricus and proteolytic L. rhamnosus. Addition of 1% glucose improved the process of fermentation. FOM was stable while stored at 4°C within seven days. Oral administration with FOM (0.4 and 2 g/kg body weight) did not alter the serum level of OVA-specific IgG and spleen index. However, FOM had comparable potency with commercial dairy yogurt to lower stimulation index, IL-2, IFN-γ and IL-4 production and to promote TGF-β and IL-10 secretion by OVA-stimulated splenocytes.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"722 - 735"},"PeriodicalIF":3.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47508325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-20DOI: 10.1080/09540105.2022.2119942
Hongyan Zhang, Qiaoying Chang, Xingzhi Wang, Jian Li, Guoyu Qiu, F. Wu, Renyuan Zhu, M. Su
ABSTRACT A decoction is the most common form of Chinese herbal medicine dosing. An Angelica sinensis decoction was qualitatively and semi-quantitatively analyzed for 15 highly toxic pesticide residues by thermal desorption electrospray ionization mass spectrometry (TD-ESI/MS) with a triple quadrupole mass spectrometer in multiple reaction monitoring mode without pretreatment. The instrument response was optimal and sensitivity was highest with the following parameters: thermal desorption temperature, 300 °C; 0.1% formic acid aqueous solution-methanol (1:1,V:V) solvent; syringe pump flow rate, 150 μL/h; and sampler length, 5 cm. Good linearity was attained within the appropriate linear range with the detection limits ranging from 0.01–0.05 mg/L. The same positive sample was verified for QuEChERS pretreatment coupled with liquid chromatography tandem mass spectrometry, showing good agreement with TD-ESI/MS. The newly developed method was demonstrated to be a rapid, green, and efficient analytical method for screening 15 highly toxic pesticide residues in Angelica sinensis decoctions.
{"title":"Rapid screening of 15 highly toxic pesticide residues in Angelica sinensis decoctions by thermal desorption electrospray ionization mass spectrometry","authors":"Hongyan Zhang, Qiaoying Chang, Xingzhi Wang, Jian Li, Guoyu Qiu, F. Wu, Renyuan Zhu, M. Su","doi":"10.1080/09540105.2022.2119942","DOIUrl":"https://doi.org/10.1080/09540105.2022.2119942","url":null,"abstract":"ABSTRACT A decoction is the most common form of Chinese herbal medicine dosing. An Angelica sinensis decoction was qualitatively and semi-quantitatively analyzed for 15 highly toxic pesticide residues by thermal desorption electrospray ionization mass spectrometry (TD-ESI/MS) with a triple quadrupole mass spectrometer in multiple reaction monitoring mode without pretreatment. The instrument response was optimal and sensitivity was highest with the following parameters: thermal desorption temperature, 300 °C; 0.1% formic acid aqueous solution-methanol (1:1,V:V) solvent; syringe pump flow rate, 150 μL/h; and sampler length, 5 cm. Good linearity was attained within the appropriate linear range with the detection limits ranging from 0.01–0.05 mg/L. The same positive sample was verified for QuEChERS pretreatment coupled with liquid chromatography tandem mass spectrometry, showing good agreement with TD-ESI/MS. The newly developed method was demonstrated to be a rapid, green, and efficient analytical method for screening 15 highly toxic pesticide residues in Angelica sinensis decoctions.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"709 - 721"},"PeriodicalIF":3.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43523789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.1080/09540105.2022.2120853
Tong Zheng, Yugang Gao, Zhilong Zhang, Xinyue Li, P. Zang, Yan Zhao, Zhongmei He
ABSTRACT Skin cancer is a malignant tumour that can spread in the skin and is a serious threat to human health. Excessive ultraviolet radiation is one of the most common factors that leads to skin cancer. The purpose of this study was to evaluate the anti-skin tumour and anti-UVB damage effects of Armillaria mellea (Vahl) P. Kumm transformed Gastrodia elata Bl products (ATGP). Gastrodia elata Blume f.glauca S. Chows from Changbai Mountain and 4 Armillaria mellea (Vahl) P. Kumm strains were used as experimental materials. First, the optimum fungal strains of Armillaria mellea used to transform gastrodin were screened. Then, the MTT assay and scratch test proved that ATGP had anti-B16 melanoma cell activity in vitro. Furthermore, the anti-UVB damage effect of ATGP was proven by a UVB damage mouse skin model and HaCat photoaging model, and its mechanism was preliminarily discussed. GRAPHICAL ABSTRACT
{"title":"A study on the anti-skin tumor and anti-UVB damage effects of Gastrodia elata Bl. Products transformed by Armillaria mellea","authors":"Tong Zheng, Yugang Gao, Zhilong Zhang, Xinyue Li, P. Zang, Yan Zhao, Zhongmei He","doi":"10.1080/09540105.2022.2120853","DOIUrl":"https://doi.org/10.1080/09540105.2022.2120853","url":null,"abstract":"ABSTRACT Skin cancer is a malignant tumour that can spread in the skin and is a serious threat to human health. Excessive ultraviolet radiation is one of the most common factors that leads to skin cancer. The purpose of this study was to evaluate the anti-skin tumour and anti-UVB damage effects of Armillaria mellea (Vahl) P. Kumm transformed Gastrodia elata Bl products (ATGP). Gastrodia elata Blume f.glauca S. Chows from Changbai Mountain and 4 Armillaria mellea (Vahl) P. Kumm strains were used as experimental materials. First, the optimum fungal strains of Armillaria mellea used to transform gastrodin were screened. Then, the MTT assay and scratch test proved that ATGP had anti-B16 melanoma cell activity in vitro. Furthermore, the anti-UVB damage effect of ATGP was proven by a UVB damage mouse skin model and HaCat photoaging model, and its mechanism was preliminarily discussed. GRAPHICAL ABSTRACT","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"736 - 751"},"PeriodicalIF":3.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42575816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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